Rroxscaffold_5G00385800

AMP deaminase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001592
Physical Location & Seq
Reverse (-)
64786438 .. 64792663
6226 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00385800.1

Sequence Viewer

Length: 1860 bp
ATGGGGAGGAATGACCCCTCTCTTGCAAGAATTCTTTCACCGTTAAGTGCACCAATGAATGAGTCAATAACCAAAGAGGATGAAGAAGTACACAGGATGATTCGAGATTGCTTGGATCTGCGTAAGAAGTACCTTTACCGGGAAATTGTTGTTCCATGGAGGGTAGCCAGGCCAGACTCATCAGAGAAGAAAAGCAACCCATATCATTTTGAACCTGTTAAAGCATCAGCTCACTGCTTTAGGATGGAGGATGGTGTCATACATGTTTATGCAAGTGAAAATGACTCTGAAGAGATCTACCCTGTTGCAAGTGCAACAACATTTTTCACTGATCTCCATTATCTTCTTAAAGTTGTGTCTATCGGAAATGTTCGCTCTGTATGCCATCATAGGTTAAGATTTCTTGACGAGAAATACCGTGTACATCATTTACTAAATGAGGGGGAGGAGTTTTTAGCTCAGAAGGGTGCACCCCACCGTGATTTTTACAATGTTAGAAAAGTTGATACACATGTGCATCACTCTGCTTGCATGAACCAGAAGCATCTTCTGAACTTCATCAAATCAAAACTGAAAAAAGAACCTGATGAGGTTGTCATATTCAGAGATGGAAAATATCTTACACTTAAGGAAGTTTTTAAGAGTTTGGACTTGACAGGGTATGATCTGAATGTTGACTTATTGGATGTTCATGCGGATAAGACTACTTTTCATCGATTTGACAAGTTCAACCTAAAGTATAATCCATGTGGACAGAGCAGACTCAGAGAGATATTCTTAAAGCAAGACAATCTTATCCAAGGACGATTTCTAGCAGAAGTAACAAAGGAAGTTTTGTCAGATCTTGAAGCCATGAAATACCAGATGGCAGAGTACAGAATTTCAGTTTATGGAAGAAAACAAAGTGAGTGGGATCAACTGGCTAGTTGGTTTGTTAACAATGACATTTATAGCGAGAATGTAGTATGGTTAATACAGTTACCACGGTTATACAACATCTACAAGAAGATGGGCATTGTTACCTCTTTCCAAAACATTTTAGATAATGTGTTCATTCCACTCTTTGAAGTCACAGTGGATCCAAATTCACATCCTCAATTACATTTGTTCCTGAAGCAGGTGGTGGGTTTTGACGTTGTAGATGATGAAAGTAGACCAGAAAGGCGTCCAACAAAACACATGCCAACACCATCTGAATGGACCAATGAATTCAATCCTGCATATTCTTATTATGCTTATTATTGCTATGCAAACTTGTACACTCTTAATAAGCTTCGTGAGTCAAAAGGAATGACTACAATAAAATTCCGGCCTCACTGTGGAGAGGCGGGTGATGTTGAACATTTGGCTGCGGGATTTCTTGTATGCCATAATATCTCACATGGGATCAATCTCCGGTATTCTCCAGTTCTGCAATATCTGTATTATCTGGGGCAGGTGGGACTGCTTATGTCACCCTTGAGCAATAATTCTCTTTTCTTAGATTACAAGAAAAACCCGTTTCCTGTATTCTTTCAACGCGGGCTAAATGTATCGCTTTCAAGCGATGATCCTTTGCTAATTCATTTGACAAAAGAAGCACTGGTGGAAGAATATAGTGTTGCAGCACAGGTATGGAAGCTCAGTGCTTGTGACCTCTGTGAGATAGCTAGAAATTCTGTCTATCATTCTGGATTTTCACATGTAGCAAAGGCACATTGGCTTGGTAGCAAGTATTTCTTGAGAGGCCCTAAAGGAAATGATATACAAAAGACGAATGTTCCCAATTTGAGGATTGCCTTTCGAGAGACGACCTGGAAGGAGGAGATGCAGTATATCTATTCAGGAAAAGCTGAGTTTTCAGAAGAGCTTGATCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko01100 ko01110 ko01130 map00230 map01100 map01110 map01130
Pfam Domains
Protein Families
A_deaminase

Protein Analysis

619

Amino Acids

72.1

Weight (kDa)

6.97

Isoelectric Point (pI)

38.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP_deaminase PF19326 26 - 606 1.3e-287 AMP deaminase
A_deaminase PF00962 434 - 560 4.7e-09 Adenosine deaminase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1108, 1426
Acc36I ACCTGC 2 cut(s) 1108, 1426
AccI GTMKAC 1 cut(s) 1153
AccII CGCG 1 cut(s) 1521
AciI CCGC 4 cut(s) 695, 1328, 1352, 1521
AclWI GGATC 7 cut(s) 123, 921, 1073, 1086, 1394, 1544, 1847
AcsI RAATTY 6 cut(s) 30, 879, 1084, 1208, 1304, 1654
AcuI CTGAAG 2 cut(s) 309, 1133
AcyI GRCGYC 1 cut(s) 1165
AfaI GTAC 5 cut(s) 90, 131, 423, 875, 1259
AfiI CCNNNNNNNGG 4 cut(s) 139, 1117, 1319, 1770
AflII CTTAAG 1 cut(s) 626
AflIII ACRYGT 3 cut(s) 262, 511, 1681
AgsI TTSAA 8 cut(s) 212, 730, 848, 1067, 1213, 1340, 1517, 1542
AjnI CCWGG 2 cut(s) 167, 1793
AjuI GAANNNNNNNTTGG 2 cut(s) 792, 824
AluBI AGCT 7 cut(s) 230, 458, 1273, 1621, 1649, 1832, 1849
AluI AGCT 7 cut(s) 230, 458, 1273, 1621, 1649, 1832, 1849
Alw21I GWGCWC 2 cut(s) 52, 472
Alw26I GTCTC 1 cut(s) 1781
Alw44I GTGCAC 2 cut(s) 48, 468
AlwI GGATC 7 cut(s) 123, 921, 1073, 1086, 1394, 1544, 1847
AoxI GGCC 3 cut(s) 170, 1310, 1726
ApaLI GTGCAC 2 cut(s) 48, 468
ApeKI GCWGC 2 cut(s) 1349, 1604
ApoI RAATTY 6 cut(s) 30, 879, 1084, 1208, 1304, 1654
Asp700I GAANNNNTTC 1 cut(s) 34
AspS9I GGNCC 2 cut(s) 1200, 1727
AsuC2I CCSGG 1 cut(s) 140
AsuHPI GGTGA 3 cut(s) 30, 1343, 1446
AvaII GGWCC 1 cut(s) 1200
BaeGI GKGCMC 2 cut(s) 52, 472
BaeI ACNNNNGTAYC 2 cut(s) 498, 531
BamHI GGATCC 1 cut(s) 1078
BarI GAAGNNNNNNTAC 2 cut(s) 119, 151
Bbv12I GWGCWC 2 cut(s) 52, 472
BbvI GCAGC 2 cut(s) 1336, 1616
BccI CCATC 7 cut(s) 238, 245, 393, 602, 859, 1003, 1198
BciT130I CCWGG 2 cut(s) 169, 1795
BcnI CCSGG 1 cut(s) 140
BcoDI GTCTC 1 cut(s) 1781
BfaI CTAG 3 cut(s) 812, 924, 1650
BfrI CTTAAG 1 cut(s) 626
BfuAI ACCTGC 2 cut(s) 1108, 1426
BglII AGATCT 2 cut(s) 294, 841
BisI GCNGC 2 cut(s) 1350, 1605
BlsI GCNGC 2 cut(s) 1351, 1606
Bme1390I CCNGG 3 cut(s) 140, 169, 1795
Bme18I GGWCC 1 cut(s) 1200
BmgT120I GGNCC 2 cut(s) 1200, 1727
BmiI GGNNCC 1 cut(s) 1080
BmrFI CCNGG 3 cut(s) 140, 169, 1795
BmsI GCATC 4 cut(s) 233, 526, 553, 1797
BpmI CTGGAG 1 cut(s) 1389
BpuEI CTTGAG 2 cut(s) 1480, 1741
BpuMI CCSGG 1 cut(s) 140
Bsa29I ATCGAT 1 cut(s) 715
BsaHI GRCGYC 1 cut(s) 1165
BsaJI CCNNGG 3 cut(s) 155, 799, 983
BsaWI WCCGGW 1 cut(s) 1395
BsaXI ACNNNNNCTCC 2 cut(s) 1796, 1826
Bsc4I CCNNNNNNNGG 4 cut(s) 139, 1117, 1319, 1770
Bse1I ACTGG 3 cut(s) 924, 1406, 1587
BseBI CCWGG 2 cut(s) 169, 1795
BseCI ATCGAT 1 cut(s) 715
BseDI CCNNGG 3 cut(s) 155, 799, 983
BseGI GGATG 6 cut(s) 85, 102, 249, 256, 691, 1090
BseLI CCNNNNNNNGG 4 cut(s) 139, 1117, 1319, 1770
BseMII CTCAG 4 cut(s) 473, 778, 1636, 1824
BseNI ACTGG 3 cut(s) 924, 1406, 1587
BseRI GAGGAG 2 cut(s) 461, 1817
BseSI GKGCMC 2 cut(s) 52, 472
BseXI GCAGC 2 cut(s) 1336, 1616
Bsh1236I CGCG 1 cut(s) 1521
BshFI GGCC 3 cut(s) 172, 1312, 1728
BshVI ATCGAT 1 cut(s) 715
BsiHKAI GWGCWC 2 cut(s) 52, 472
BsiSI CCGG 3 cut(s) 139, 1309, 1396
BslFI GGGAC 1 cut(s) 1455
BslI CCNNNNNNNGG 4 cut(s) 139, 1117, 1319, 1770
BsmAI GTCTC 1 cut(s) 1781
BsmBI CGTCTC 1 cut(s) 1781
BsmFI GGGAC 1 cut(s) 1455
BsnI GGCC 3 cut(s) 172, 1312, 1728
Bsp1286I GDGCHC 2 cut(s) 52, 472
Bsp1407I TGTACA 2 cut(s) 421, 1257
Bsp19I CCATGG 1 cut(s) 155
BspACI CCGC 4 cut(s) 695, 1328, 1352, 1521
BspANI GGCC 3 cut(s) 172, 1312, 1728
BspCNI CTCAG 4 cut(s) 472, 777, 1635, 1825
BspDI ATCGAT 1 cut(s) 715
BspFNI CGCG 1 cut(s) 1521
BspLI GGNNCC 1 cut(s) 1080
BspMI ACCTGC 2 cut(s) 1108, 1426
BspPI GGATC 7 cut(s) 123, 921, 1073, 1086, 1394, 1544, 1847
BspQI GCTCTTC 1 cut(s) 1839
BspTI CTTAAG 1 cut(s) 626
BsrGI TGTACA 2 cut(s) 421, 1257
BsrI ACTGG 3 cut(s) 924, 1406, 1587
BssECI CCNNGG 3 cut(s) 155, 799, 983
BssNI GRCGYC 1 cut(s) 1165
BssT1I CCWWGG 2 cut(s) 155, 799
Bst2UI CCWGG 2 cut(s) 169, 1795
Bst4CI ACNGT 7 cut(s) 42, 419, 479, 978, 987, 1075, 1319
Bst6I CTCTTC 2 cut(s) 285, 1839
BstACI GRCGYC 1 cut(s) 1165
BstAFI CTTAAG 1 cut(s) 626
BstAUI TGTACA 2 cut(s) 421, 1257
BstC8I GCNNGC 2 cut(s) 529, 1523
BstDEI CTNAG 5 cut(s) 459, 764, 1480, 1622, 1833
BstDSI CCRYGG 2 cut(s) 155, 983
BstENI CCTNNNNNAGG 1 cut(s) 1115
BstF5I GGATG 6 cut(s) 85, 102, 249, 256, 691, 1090
BstFNI CGCG 1 cut(s) 1521
BstMAI GTCTC 1 cut(s) 1781
BstMWI GCNNNNNNNGC 1 cut(s) 381
BstNI CCWGG 2 cut(s) 169, 1795
BstNSI RCATGY 4 cut(s) 266, 515, 1183, 1685
BstSCI CCNGG 3 cut(s) 138, 167, 1793
BstSLI GKGCMC 2 cut(s) 52, 472
BstUI CGCG 1 cut(s) 1521
BstV1I GCAGC 2 cut(s) 1336, 1616
BstX2I RGATCY 4 cut(s) 115, 294, 841, 1078
BstXI CCANNNNNNTGG 1 cut(s) 1197
BstYI RGATCY 4 cut(s) 115, 294, 841, 1078
Bsu15I ATCGAT 1 cut(s) 715
BsuRI GGCC 3 cut(s) 172, 1312, 1728
BsuTUI ATCGAT 1 cut(s) 715
BtgI CCRYGG 2 cut(s) 155, 983
BtgZI GCGATG 1 cut(s) 1560
BtsCI GGATG 6 cut(s) 85, 102, 249, 256, 691, 1090
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 6 cut(s) 232, 327, 1080, 1315, 1580, 1630
BveI ACCTGC 2 cut(s) 1108, 1426
Cac8I GCNNGC 2 cut(s) 529, 1523
Cfr13I GGNCC 2 cut(s) 1200, 1727
ClaI ATCGAT 1 cut(s) 715
CseI GACGC 1 cut(s) 1154
Csp6I GTAC 5 cut(s) 89, 130, 422, 874, 1258
CspCI CAANNNNNGTGG 2 cut(s) 890, 925
CviQI GTAC 5 cut(s) 89, 130, 422, 874, 1258
DdeI CTNAG 5 cut(s) 459, 764, 1480, 1622, 1833
Eam1104I CTCTTC 2 cut(s) 285, 1839
EarI CTCTTC 2 cut(s) 285, 1839
Eco130I CCWWGG 2 cut(s) 155, 799
Eco47I GGWCC 1 cut(s) 1200
Eco57I CTGAAG 2 cut(s) 309, 1133
EcoNI CCTNNNNNAGG 1 cut(s) 1115
EcoO109I RGGNCCY 1 cut(s) 1727
EcoRI GAATTC 2 cut(s) 30, 1208
EcoRII CCWGG 2 cut(s) 167, 1793
EcoT14I CCWWGG 2 cut(s) 155, 799
ErhI CCWWGG 2 cut(s) 155, 799
Esp3I CGTCTC 1 cut(s) 1781
FalI AAGNNNNNCTT 4 cut(s) 777, 809, 1703, 1735
FaqI GGGAC 1 cut(s) 1455
FauI CCCGC 3 cut(s) 1321, 1345, 1514
FblI GTMKAC 1 cut(s) 1153
Fnu4HI GCNGC 2 cut(s) 1350, 1605
FokI GGATG 6 cut(s) 92, 109, 256, 263, 698, 1077
Fsp4HI GCNGC 2 cut(s) 1350, 1605
FspBI CTAG 3 cut(s) 812, 924, 1650
GluI GCNGC 2 cut(s) 1350, 1605
GsuI CTGGAG 1 cut(s) 1389
HaeIII GGCC 3 cut(s) 172, 1312, 1728
HapII CCGG 3 cut(s) 139, 1309, 1396
HgaI GACGC 1 cut(s) 1154
Hin1I GRCGYC 1 cut(s) 1165
HincII GTYRAC 2 cut(s) 676, 937
HindII GTYRAC 2 cut(s) 676, 937
HindIII AAGCTT 1 cut(s) 1271
HinfI GANTC 6 cut(s) 62, 100, 176, 284, 762, 1280
HpaI GTTAAC 1 cut(s) 937
HpaII CCGG 3 cut(s) 139, 1309, 1396
HphI GGTGA 3 cut(s) 30, 1343, 1446
Hpy166II GTNNAC 9 cut(s) 50, 91, 422, 470, 676, 752, 937, 1154, 1260
Hpy188III TCNNGA 9 cut(s) 104, 404, 845, 1111, 1277, 1671, 1720, 1784, 1824
Hpy8I GTNNAC 9 cut(s) 50, 91, 422, 470, 676, 752, 937, 1154, 1260
HpyAV CCTTC 2 cut(s) 457, 1792
HpyCH4III ACNGT 7 cut(s) 42, 419, 479, 978, 987, 1075, 1319
HpyCH4IV ACGT 1 cut(s) 1134
HpyF10VI GCNNNNNNNGC 1 cut(s) 381
HpyF3I CTNAG 5 cut(s) 459, 764, 1480, 1622, 1833
HpySE526I ACGT 1 cut(s) 1134
Hsp92I GRCGYC 1 cut(s) 1165
KspAI GTTAAC 1 cut(s) 937
LguI GCTCTTC 1 cut(s) 1839
Lsp1109I GCAGC 2 cut(s) 1336, 1616
LweI GCATC 4 cut(s) 233, 526, 553, 1797
MaeI CTAG 3 cut(s) 812, 924, 1650
MaeII ACGT 1 cut(s) 1134
MaeIII GTNAC 6 cut(s) 820, 978, 1018, 1069, 1452, 1631
MboII GAAGA 9 cut(s) 95, 199, 302, 335, 539, 906, 1018, 1601, 1856
MflI RGATCY 4 cut(s) 115, 294, 841, 1078
MhlI GDGCHC 2 cut(s) 52, 472
MlyI GAGTC 5 cut(s) 71, 170, 278, 756, 1289
MmeI TCCRAC 1 cut(s) 1193
MroXI GAANNNNTTC 1 cut(s) 34
MslI CAYNNNNRTG 3 cut(s) 267, 1195, 1612
MspCI CTTAAG 1 cut(s) 626
MspI CCGG 3 cut(s) 139, 1309, 1396
MspR9I CCNGG 3 cut(s) 140, 169, 1795
MvaI CCWGG 2 cut(s) 169, 1795
MvnI CGCG 1 cut(s) 1521
MwoI GCNNNNNNNGC 1 cut(s) 381
NciI CCSGG 1 cut(s) 140
NcoI CCATGG 1 cut(s) 155
NlaIV GGNNCC 1 cut(s) 1080
NmuCI GTSAC 3 cut(s) 1069, 1452, 1631
NspI RCATGY 4 cut(s) 266, 515, 1183, 1685
PaqCI CACCTGC 2 cut(s) 1108, 1426
PciI ACATGT 3 cut(s) 262, 511, 1681
PciSI GCTCTTC 1 cut(s) 1839
PdmI GAANNNNTTC 1 cut(s) 34
PfeI GAWTC 1 cut(s) 100
PkrI GCNGC 2 cut(s) 1351, 1606
PleI GAGTC 5 cut(s) 70, 170, 278, 756, 1288
PpsI GAGTC 5 cut(s) 70, 170, 278, 756, 1288
PscI ACATGT 3 cut(s) 262, 511, 1681
Psp6I CCWGG 2 cut(s) 167, 1793
PspGI CCWGG 2 cut(s) 167, 1793
PspN4I GGNNCC 1 cut(s) 1080
PspPI GGNCC 2 cut(s) 1200, 1727
PsuI RGATCY 4 cut(s) 115, 294, 841, 1078
RsaI GTAC 5 cut(s) 90, 131, 423, 875, 1259
RsaNI GTAC 5 cut(s) 89, 130, 422, 874, 1258
RseI CAYNNNNRTG 3 cut(s) 267, 1195, 1612
SapI GCTCTTC 1 cut(s) 1839
SatI GCNGC 2 cut(s) 1350, 1605
Sau96I GGNCC 2 cut(s) 1200, 1727
SchI GAGTC 5 cut(s) 71, 170, 278, 756, 1289
ScrFI CCNGG 3 cut(s) 140, 169, 1795
SduI GDGCHC 2 cut(s) 52, 472
SfaNI GCATC 4 cut(s) 233, 526, 553, 1797
SinI GGWCC 1 cut(s) 1200
SmiMI CAYNNNNRTG 3 cut(s) 267, 1195, 1612
SmlI CTYRAG 3 cut(s) 626, 1459, 1720
SmoI CTYRAG 3 cut(s) 626, 1459, 1720
SsiI CCGC 4 cut(s) 695, 1328, 1352, 1521
SspMI CTAG 3 cut(s) 812, 924, 1650
StyD4I CCNGG 3 cut(s) 138, 167, 1793
StyI CCWWGG 2 cut(s) 155, 799
TaaI ACNGT 7 cut(s) 42, 419, 479, 978, 987, 1075, 1319
TaiI ACGT 1 cut(s) 1137
TaqI TCGA 3 cut(s) 103, 715, 1783
TatI WGTACW 4 cut(s) 88, 421, 873, 1257
TfiI GAWTC 1 cut(s) 100
TscAI CASTG 6 cut(s) 239, 334, 1080, 1322, 1587, 1630
TseFI GTSAC 3 cut(s) 1069, 1452, 1631
TseI GCWGC 2 cut(s) 1349, 1604
Tsp45I GTSAC 3 cut(s) 1069, 1452, 1631
TspRI CASTG 6 cut(s) 239, 334, 1080, 1322, 1587, 1630
Vha464I CTTAAG 1 cut(s) 626
VneI GTGCAC 2 cut(s) 48, 468
VpaK11BI GGWCC 1 cut(s) 1200
XagI CCTNNNNNAGG 1 cut(s) 1115
XapI RAATTY 6 cut(s) 30, 879, 1084, 1208, 1304, 1654
XceI RCATGY 4 cut(s) 266, 515, 1183, 1685
XmiI GTMKAC 1 cut(s) 1153
XmnI GAANNNNTTC 1 cut(s) 34
XspI CTAG 3 cut(s) 812, 924, 1650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.