Rroxscaffold_6G00430510

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0011566
Physical Location & Seq
Forward (+)
50637922 .. 50638308
387 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00430510.1

Sequence Viewer

Length: 387 bp
ATGGAGTGCGGGGGATCCGTAGGCAAATGTCGACGGATGCTCGCGGTGTGCGCGGGGGCTCGGCGAGTGTGTGCGGGGTCGTCGGCGGGGGCAGGCACACGTCTCGGCAGGTGCGAGTTGGGCTTCTCGGTAGATGTCGGCAGCACTCGGCATGTCTCGGCAGGAAGCATGCGGGGCTCTAGGCGGATGCTTGCGGGGGCGTCGACGAGAGGCTCGACGGCACTTGATAGATCTCGGCAGGGGCGTGCGGGGACCTCGGCGAGTGCCGGTCCGATTTTTGGACGGCCGGTTCGGGGCTTTCCGGCCGGTTCCGGTGGTTCCACCGCCGGTTCTGGGTTCCTTGAGGCTAAGGCTTCAATATGTGGGGCGGTGGATGTTGGAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

128

Amino Acids

12.56

Weight (kDa)

11.38

Isoelectric Point (pI)

32.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 99
Acc36I ACCTGC 1 cut(s) 99
AccI GTMKAC 2 cut(s) 31, 203
AccII CGCG 2 cut(s) 44, 53
AclWI GGATC 2 cut(s) 9, 22
AcoI YGGCCR 2 cut(s) 284, 303
AcsI RAATTY 1 cut(s) 381
AcyI GRCGYC 1 cut(s) 200
AfiI CCNNNNNNNGG 3 cut(s) 278, 293, 333
AflIII ACRYGT 1 cut(s) 98
AgsI TTSAA 1 cut(s) 357
AjiI CACGTC 1 cut(s) 101
AloI GAACNNNNNNTCC 2 cut(s) 273, 305
Alw26I GTCTC 2 cut(s) 107, 160
AlwI GGATC 2 cut(s) 9, 22
AoxI GGCC 2 cut(s) 284, 303
ApeKI GCWGC 1 cut(s) 141
ApoI RAATTY 1 cut(s) 381
ArsI GACNNNNNNTTYG 2 cut(s) 273, 305
AspLEI GCGC 1 cut(s) 53
AspS9I GGNCC 2 cut(s) 252, 269
AvaII GGWCC 2 cut(s) 252, 269
BamHI GGATCC 1 cut(s) 14
BanII GRGCYC 2 cut(s) 61, 179
BbvI GCAGC 1 cut(s) 153
BceAI ACGGC 2 cut(s) 234, 299
BcgI CGANNNNNNTGC 2 cut(s) 85, 119
BcoDI GTCTC 2 cut(s) 107, 160
BfaI CTAG 1 cut(s) 180
BfuAI ACCTGC 1 cut(s) 99
BglII AGATCT 1 cut(s) 230
BisI GCNGC 1 cut(s) 142
BlsI GCNGC 1 cut(s) 143
Bme18I GGWCC 2 cut(s) 252, 269
BmgBI CACGTC 1 cut(s) 101
BmgT120I GGNCC 2 cut(s) 252, 269
BmiI GGNNCC 5 cut(s) 16, 253, 310, 319, 338
BmsI GCATC 2 cut(s) 27, 177
Bpu10I CCTNAGC 1 cut(s) 348
BpuEI CTTGAG 1 cut(s) 362
BsaHI GRCGYC 1 cut(s) 200
BsaJI CCNNGG 1 cut(s) 255
BsaWI WCCGGW 1 cut(s) 311
Bsc4I CCNNNNNNNGG 3 cut(s) 278, 293, 333
Bse118I RCCGGY 4 cut(s) 266, 286, 305, 326
BseDI CCNNGG 1 cut(s) 255
BseGI GGATG 3 cut(s) 42, 192, 379
BseLI CCNNNNNNNGG 3 cut(s) 278, 293, 333
BseX3I CGGCCG 2 cut(s) 284, 303
BseXI GCAGC 1 cut(s) 153
Bsh1236I CGCG 2 cut(s) 44, 53
Bsh1285I CGRYCG 2 cut(s) 287, 306
BshFI GGCC 2 cut(s) 286, 305
BsiEI CGRYCG 2 cut(s) 287, 306
BsiSI CCGG 6 cut(s) 267, 287, 302, 306, 312, 327
BslFI GGGAC 1 cut(s) 265
BslI CCNNNNNNNGG 3 cut(s) 278, 293, 333
BsmAI GTCTC 2 cut(s) 107, 160
BsmBI CGTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 265
BsnI GGCC 2 cut(s) 286, 305
Bsp1286I GDGCHC 2 cut(s) 61, 179
Bsp143I GATC 2 cut(s) 14, 230
BspANI GGCC 2 cut(s) 286, 305
BspFNI CGCG 2 cut(s) 44, 53
BspLI GGNNCC 5 cut(s) 16, 253, 310, 319, 338
BspMI ACCTGC 1 cut(s) 99
BspPI GGATC 2 cut(s) 9, 22
BsrFI RCCGGY 4 cut(s) 266, 286, 305, 326
BssAI RCCGGY 4 cut(s) 266, 286, 305, 326
BssECI CCNNGG 1 cut(s) 255
BssMI GATC 2 cut(s) 14, 230
BssNI GRCGYC 1 cut(s) 200
BstACI GRCGYC 1 cut(s) 200
BstC8I GCNNGC 5 cut(s) 42, 94, 170, 192, 246
BstDEI CTNAG 1 cut(s) 348
BstF5I GGATG 3 cut(s) 42, 192, 379
BstFNI CGCG 2 cut(s) 44, 53
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 2 cut(s) 17, 233
BstMAI GTCTC 2 cut(s) 107, 160
BstMBI GATC 2 cut(s) 14, 230
BstMCI CGRYCG 2 cut(s) 287, 306
BstMWI GCNNNNNNNGC 3 cut(s) 50, 120, 174
BstNSI RCATGY 2 cut(s) 155, 172
BstUI CGCG 2 cut(s) 44, 53
BstV1I GCAGC 1 cut(s) 153
BstX2I RGATCY 2 cut(s) 14, 230
BstYI RGATCY 2 cut(s) 14, 230
BstZI CGGCCG 2 cut(s) 284, 303
BsuRI GGCC 2 cut(s) 286, 305
BtrI CACGTC 1 cut(s) 101
BtsCI GGATG 3 cut(s) 42, 192, 379
BveI ACCTGC 1 cut(s) 99
Cac8I GCNNGC 5 cut(s) 42, 94, 170, 192, 246
CfoI GCGC 1 cut(s) 53
Cfr10I RCCGGY 4 cut(s) 266, 286, 305, 326
Cfr13I GGNCC 2 cut(s) 252, 269
CpoI CGGWCCG 1 cut(s) 269
CseI GACGC 1 cut(s) 189
CspI CGGWCCG 1 cut(s) 269
CviAII CATG 2 cut(s) 152, 169
CviJI RGCY 9 cut(s) 59, 123, 177, 213, 286, 297, 305, 347, 353
CviKI_1 RGCY 9 cut(s) 59, 123, 177, 213, 286, 297, 305, 347, 353
DdeI CTNAG 1 cut(s) 348
DpnI GATC 2 cut(s) 16, 232
DpnII GATC 2 cut(s) 14, 230
EaeI YGGCCR 2 cut(s) 284, 303
EagI CGGCCG 2 cut(s) 284, 303
EciI GGCGGA 1 cut(s) 199
EclXI CGGCCG 2 cut(s) 284, 303
Eco24I GRGCYC 2 cut(s) 61, 179
Eco47I GGWCC 2 cut(s) 252, 269
Eco52I CGGCCG 2 cut(s) 284, 303
EcoO109I RGGNCCY 1 cut(s) 252
EcoT38I GRGCYC 2 cut(s) 61, 179
Esp3I CGTCTC 1 cut(s) 107
FaeI CATG 2 cut(s) 155, 172
FaiI YATR 3 cut(s) 153, 170, 361
FaqI GGGAC 1 cut(s) 265
FatI CATG 2 cut(s) 151, 168
FauI CCCGC 7 cut(s) 2, 46, 67, 79, 165, 187, 241
FblI GTMKAC 2 cut(s) 31, 203
Fnu4HI GCNGC 1 cut(s) 142
FokI GGATG 2 cut(s) 49, 199
FriOI GRGCYC 2 cut(s) 61, 179
Fsp4HI GCNGC 1 cut(s) 142
FspBI CTAG 1 cut(s) 180
GlaI GCGC 1 cut(s) 52
GluI GCNGC 1 cut(s) 142
HaeIII GGCC 2 cut(s) 286, 305
HapII CCGG 6 cut(s) 267, 287, 302, 306, 312, 327
HgaI GACGC 1 cut(s) 189
HhaI GCGC 1 cut(s) 53
Hin1I GRCGYC 1 cut(s) 200
Hin1II CATG 2 cut(s) 155, 172
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HincII GTYRAC 2 cut(s) 32, 204
HindII GTYRAC 2 cut(s) 32, 204
HpaII CCGG 6 cut(s) 267, 287, 302, 306, 312, 327
Hpy166II GTNNAC 2 cut(s) 32, 204
Hpy188I TCNGA 1 cut(s) 273
Hpy8I GTNNAC 2 cut(s) 32, 204
Hpy99I CGWCG 5 cut(s) 36, 85, 205, 208, 220
HpyCH4IV ACGT 1 cut(s) 100
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 120, 174
HpyF3I CTNAG 1 cut(s) 348
HpySE526I ACGT 1 cut(s) 100
Hsp92I GRCGYC 1 cut(s) 200
Hsp92II CATG 2 cut(s) 155, 172
HspAI GCGC 1 cut(s) 51
Kzo9I GATC 2 cut(s) 14, 230
Lsp1109I GCAGC 1 cut(s) 153
LweI GCATC 2 cut(s) 27, 177
MaeI CTAG 1 cut(s) 180
MaeII ACGT 1 cut(s) 100
MalI GATC 2 cut(s) 16, 232
MboI GATC 2 cut(s) 14, 230
MflI RGATCY 2 cut(s) 14, 230
MhlI GDGCHC 2 cut(s) 61, 179
MluCI AATT 1 cut(s) 381
MmeI TCCRAC 1 cut(s) 358
MnlI CCTC 3 cut(s) 203, 265, 337
MspI CCGG 6 cut(s) 267, 287, 302, 306, 312, 327
MvnI CGCG 2 cut(s) 44, 53
MwoI GCNNNNNNNGC 3 cut(s) 50, 120, 174
NdeII GATC 2 cut(s) 14, 230
NlaIII CATG 2 cut(s) 155, 172
NlaIV GGNNCC 5 cut(s) 16, 253, 310, 319, 338
NmeAIII GCCGAG 6 cut(s) 40, 84, 127, 137, 214, 236
NspI RCATGY 2 cut(s) 155, 172
PaeI GCATGC 1 cut(s) 172
PaqCI CACCTGC 1 cut(s) 99
PcsI WCGNNNNNNNCGW 3 cut(s) 111, 212, 241
PkrI GCNGC 1 cut(s) 143
PpuMI RGGWCCY 1 cut(s) 252
Psp5II RGGWCCY 1 cut(s) 252
PspN4I GGNNCC 5 cut(s) 16, 253, 310, 319, 338
PspPI GGNCC 2 cut(s) 252, 269
PspPPI RGGWCCY 1 cut(s) 252
PsuI RGATCY 2 cut(s) 14, 230
Rsr2I CGGWCCG 1 cut(s) 269
RsrII CGGWCCG 1 cut(s) 269
SalI GTCGAC 2 cut(s) 30, 202
SatI GCNGC 1 cut(s) 142
Sau3AI GATC 2 cut(s) 14, 230
Sau96I GGNCC 2 cut(s) 252, 269
SduI GDGCHC 2 cut(s) 61, 179
SetI ASST 3 cut(s) 103, 113, 257
SfaNI GCATC 2 cut(s) 27, 177
SgrDI CGTCGACG 1 cut(s) 202
SinI GGWCC 2 cut(s) 252, 269
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
SphI GCATGC 1 cut(s) 172
Sse9I AATT 1 cut(s) 381
SspMI CTAG 1 cut(s) 180
TaiI ACGT 1 cut(s) 103
TaqI TCGA 3 cut(s) 31, 203, 215
TasI AATT 1 cut(s) 381
TseI GCWGC 1 cut(s) 141
TspGWI ACGGA 2 cut(s) 7, 49
VpaK11BI GGWCC 2 cut(s) 252, 269
XapI RAATTY 1 cut(s) 381
XceI RCATGY 2 cut(s) 155, 172
XmiI GTMKAC 2 cut(s) 31, 203
XspI CTAG 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.