Rroxscaffold_7G00190510

peroxisomal (S)-2-hydroxy-acid oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002723
Physical Location & Seq
Forward (+)
30653217 .. 30655426
2210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00190510.1

Sequence Viewer

Length: 681 bp
ATGGATCACATAACCAATGTTTCTGAGTATGAAGCAATTGCAAAGAAGAATTTGCCGAAGATGCTTTACGACTACTATGCATCCGGTGCAGAGGACCAGTGGACTCTTAAGGAGAATCTACTTGCCTTTTCCAGGATTTTGTTTCGACCTCGAATTCTTATTGATGTAAGCACAATAGACTTGAGCACAACTATTTTGAGCTTCAAAGTTTCAATGCCCATCATGATTGCTCCTACAGGCATGCAGAAGATGGCTCACCCTGAAGGAGAGTGTGCAACCGCAAGAGCAGCATCTGCAGCTGGCACAATTATGACACTGTCCACTATGGCTAATTCCAGCATTGAAGAAGTTGCCTCAACAGGACCTGGTATTTGCTTGTTCCAACTCTATGTGTTGAAAGACAGGAATCTGGTTACACAGCTTGTCAGAAGGGCTGAGAGGGCTGGTTACAAGGCAATAGTCCTCACTGCTGACACTCCGAGGCTCGGGCGCAGGGAGGCTGATATCAAGAACAGGTTTACTATGTCACCAAGTTTGACATTGAAAAACTTCGAAAATTTGGATATTGGAAAGATTGATGAGACCAATGGTTCTGGATTAGCATCATATGTTGCTGGACTATTTGACCGCTCTCTCAGCTGGAAGCAAGGTTATGCATACAAGCTGGAGCTGCAGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00630 ko01100 ko01110 ko01120 ko01130 ko01200 ko04146 map00630 map01100 map01110 map01120 map01130 map01200 map04146
Pfam Domains
Protein Families
FMN_dh

Protein Analysis

226

Amino Acids

25.09

Weight (kDa)

7.71

Isoelectric Point (pI)

38.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMN_dh PF01070 14 - 216 2.9e-64 FMN-dependent dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 630
AciI CCGC 2 cut(s) 279, 628
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 3 cut(s) 49, 153, 556
AcuI CTGAAG 1 cut(s) 282
AfiI CCNNNNNNNGG 2 cut(s) 132, 485
AflII CTTAAG 1 cut(s) 107
AgsI TTSAA 5 cut(s) 205, 213, 344, 397, 544
AjnI CCWGG 2 cut(s) 131, 364
AluBI AGCT 6 cut(s) 201, 299, 421, 639, 664, 670
AluI AGCT 6 cut(s) 201, 299, 421, 639, 664, 670
Alw21I GWGCWC 1 cut(s) 188
Alw26I GTCTC 1 cut(s) 575
AlwI GGATC 1 cut(s) 12
AlwNI CAGNNNCTG 2 cut(s) 293, 365
Ama87I CYCGRG 1 cut(s) 485
ApeKI GCWGC 3 cut(s) 287, 296, 670
ApoI RAATTY 3 cut(s) 49, 153, 556
ArsI GACNNNNNNTTYG 2 cut(s) 354, 386
Asp700I GAANNNNTTC 1 cut(s) 548
AspLEI GCGC 1 cut(s) 492
AspS9I GGNCC 2 cut(s) 94, 362
AsuHPI GGTGA 2 cut(s) 248, 519
AsuII TTCGAA 1 cut(s) 552
AvaI CYCGRG 1 cut(s) 485
AvaII GGWCC 2 cut(s) 94, 362
BarI GAAGNNNNNNTAC 2 cut(s) 50, 82
Bbv12I GWGCWC 1 cut(s) 188
BbvI GCAGC 3 cut(s) 299, 308, 657
BccI CCATC 2 cut(s) 227, 244
BcgI CGANNNNNNTGC 2 cut(s) 59, 93
BciT130I CCWGG 2 cut(s) 133, 366
BcoDI GTCTC 1 cut(s) 575
BfmI CTRYAG 3 cut(s) 234, 294, 671
BfrI CTTAAG 1 cut(s) 107
BisI GCNGC 3 cut(s) 288, 297, 671
BlsI GCNGC 3 cut(s) 289, 298, 672
Bme1390I CCNGG 2 cut(s) 133, 366
Bme18I GGWCC 2 cut(s) 94, 362
BmeT110I CYCGRG 1 cut(s) 485
BmgT120I GGNCC 2 cut(s) 94, 362
BmrFI CCNGG 2 cut(s) 133, 366
BmsI GCATC 4 cut(s) 51, 89, 299, 611
Bpu14I TTCGAA 1 cut(s) 552
BpuEI CTTGAG 1 cut(s) 202
BsaBI GATNNNNATC 1 cut(s) 601
BsaI GGTCTC 1 cut(s) 575
BsaJI CCNNGG 1 cut(s) 479
BsaWI WCCGGW 1 cut(s) 83
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 485
Bse1I ACTGG 1 cut(s) 97
Bse8I GATNNNNATC 1 cut(s) 601
BseBI CCWGG 2 cut(s) 133, 366
BseDI CCNNGG 1 cut(s) 479
BseGI GGATG 1 cut(s) 80
BseJI GATNNNNATC 1 cut(s) 601
BseLI CCNNNNNNNGG 2 cut(s) 132, 485
BseMII CTCAG 3 cut(s) 15, 426, 649
BseNI ACTGG 1 cut(s) 97
BseXI GCAGC 3 cut(s) 299, 308, 657
BsgI GTGCAG 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 188
BsiHKCI CYCGRG 1 cut(s) 485
BsiSI CCGG 1 cut(s) 84
BslI CCNNNNNNNGG 2 cut(s) 132, 485
BsmAI GTCTC 1 cut(s) 575
Bso31I GGTCTC 1 cut(s) 575
BsoBI CYCGRG 1 cut(s) 485
Bsp119I TTCGAA 1 cut(s) 552
Bsp1286I GDGCHC 1 cut(s) 188
Bsp143I GATC 1 cut(s) 4
BspACI CCGC 2 cut(s) 279, 628
BspCNI CTCAG 3 cut(s) 16, 427, 648
BspHI TCATGA 1 cut(s) 222
BspMAI CTGCAG 2 cut(s) 298, 675
BspPI GGATC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 552
BspTI CTTAAG 1 cut(s) 107
BspTNI GGTCTC 1 cut(s) 575
BsrBI CCGCTC 1 cut(s) 630
BsrI ACTGG 1 cut(s) 97
BssECI CCNNGG 1 cut(s) 479
BssMI GATC 1 cut(s) 4
Bst2UI CCWGG 2 cut(s) 133, 366
Bst4CI ACNGT 1 cut(s) 318
BstAFI CTTAAG 1 cut(s) 107
BstAPI GCANNNNNTGC 2 cut(s) 86, 293
BstBI TTCGAA 1 cut(s) 552
BstC8I GCNNGC 2 cut(s) 242, 301
BstDEI CTNAG 3 cut(s) 24, 435, 635
BstENI CCTNNNNNAGG 1 cut(s) 130
BstF5I GGATG 1 cut(s) 80
BstHHI GCGC 1 cut(s) 492
BstKTI GATC 1 cut(s) 7
BstMAI GTCTC 1 cut(s) 575
BstMBI GATC 1 cut(s) 4
BstMWI GCNNNNNNNGC 8 cut(s) 61, 86, 287, 293, 296, 440, 636, 670
BstNI CCWGG 2 cut(s) 133, 366
BstNSI RCATGY 1 cut(s) 244
BstSCI CCNGG 2 cut(s) 131, 364
BstSFI CTRYAG 3 cut(s) 234, 294, 671
BstV1I GCAGC 3 cut(s) 299, 308, 657
BtsCI GGATG 1 cut(s) 80
BtsI GCAGTG 1 cut(s) 465
BtsIMutI CAGTG 3 cut(s) 104, 314, 465
Cac8I GCNNGC 2 cut(s) 242, 301
CaiI CAGNNNCTG 2 cut(s) 293, 365
CciI TCATGA 1 cut(s) 222
CfoI GCGC 1 cut(s) 492
Cfr13I GGNCC 2 cut(s) 94, 362
CsiI ACCWGGT 1 cut(s) 364
CviAII CATG 2 cut(s) 223, 241
DdeI CTNAG 3 cut(s) 24, 435, 635
DpnI GATC 1 cut(s) 6
DpnII GATC 1 cut(s) 4
Eco31I GGTCTC 1 cut(s) 575
Eco32I GATATC 1 cut(s) 505
Eco47I GGWCC 2 cut(s) 94, 362
Eco57I CTGAAG 1 cut(s) 282
Eco88I CYCGRG 1 cut(s) 485
EcoNI CCTNNNNNAGG 1 cut(s) 130
EcoO109I RGGNCCY 1 cut(s) 362
EcoRI GAATTC 1 cut(s) 153
EcoRII CCWGG 2 cut(s) 131, 364
EcoRV GATATC 1 cut(s) 505
EcoT22I ATGCAT 2 cut(s) 82, 658
FaeI CATG 2 cut(s) 226, 244
FatI CATG 2 cut(s) 222, 240
FauNDI CATATG 1 cut(s) 607
Fnu4HI GCNGC 3 cut(s) 288, 297, 671
FokI GGATG 1 cut(s) 67
Fsp4HI GCNGC 3 cut(s) 288, 297, 671
GlaI GCGC 1 cut(s) 491
GluI GCNGC 3 cut(s) 288, 297, 671
HapII CCGG 1 cut(s) 84
HhaI GCGC 1 cut(s) 492
Hin1II CATG 2 cut(s) 226, 244
Hin6I GCGC 1 cut(s) 490
HinP1I GCGC 1 cut(s) 490
HinfI GANTC 3 cut(s) 103, 115, 406
HpaII CCGG 1 cut(s) 84
HphI GGTGA 2 cut(s) 248, 519
Hpy166II GTNNAC 3 cut(s) 102, 321, 519
Hpy188I TCNGA 3 cut(s) 25, 428, 480
Hpy188III TCNNGA 3 cut(s) 223, 508, 594
Hpy8I GTNNAC 3 cut(s) 102, 321, 519
HpyAV CCTTC 2 cut(s) 257, 423
HpyCH4III ACNGT 1 cut(s) 318
HpyCH4V TGCA 8 cut(s) 41, 80, 89, 244, 275, 296, 656, 673
HpyF10VI GCNNNNNNNGC 8 cut(s) 61, 86, 287, 293, 296, 440, 636, 670
HpyF3I CTNAG 3 cut(s) 24, 435, 635
Hsp92II CATG 2 cut(s) 226, 244
HspAI GCGC 1 cut(s) 490
Kzo9I GATC 1 cut(s) 4
LmnI GCTCC 2 cut(s) 235, 667
Lsp1109I GCAGC 3 cut(s) 299, 308, 657
LweI GCATC 4 cut(s) 51, 89, 299, 611
MabI ACCWGGT 1 cut(s) 364
MaeIII GTNAC 3 cut(s) 412, 446, 525
MalI GATC 1 cut(s) 6
MbiI CCGCTC 1 cut(s) 630
MboI GATC 1 cut(s) 4
MboII GAAGA 4 cut(s) 58, 70, 259, 356
MfeI CAATTG 1 cut(s) 36
MhlI GDGCHC 1 cut(s) 188
MluCI AATT 6 cut(s) 36, 49, 153, 306, 331, 556
MlyI GAGTC 1 cut(s) 97
MmeI TCCRAC 1 cut(s) 406
MnlI CCTC 7 cut(s) 85, 159, 364, 432, 473, 474, 490
Mph1103I ATGCAT 2 cut(s) 82, 658
MroXI GAANNNNTTC 1 cut(s) 548
MseI TTAA 1 cut(s) 108
MslI CAYNNNNRTG 1 cut(s) 308
MspA1I CMGCKG 2 cut(s) 299, 639
MspCI CTTAAG 1 cut(s) 107
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 2 cut(s) 133, 366
MunI CAATTG 1 cut(s) 36
MvaI CCWGG 2 cut(s) 133, 366
MwoI GCNNNNNNNGC 8 cut(s) 61, 86, 287, 293, 296, 440, 636, 670
NdeI CATATG 1 cut(s) 607
NdeII GATC 1 cut(s) 4
NlaIII CATG 2 cut(s) 226, 244
NmuCI GTSAC 1 cut(s) 525
NsiI ATGCAT 2 cut(s) 82, 658
NspI RCATGY 1 cut(s) 244
NspV TTCGAA 1 cut(s) 552
PaeI GCATGC 1 cut(s) 244
PagI TCATGA 1 cut(s) 222
PdmI GAANNNNTTC 1 cut(s) 548
PfeI GAWTC 2 cut(s) 115, 406
PflFI GACNNNGTC 1 cut(s) 316
PfoI TCCNGGA 1 cut(s) 131
PkrI GCNGC 3 cut(s) 289, 298, 672
PleI GAGTC 1 cut(s) 97
PpsI GAGTC 1 cut(s) 97
PpuMI RGGWCCY 1 cut(s) 362
Psp5II RGGWCCY 1 cut(s) 362
Psp6I CCWGG 2 cut(s) 131, 364
PspGI CCWGG 2 cut(s) 131, 364
PspPI GGNCC 2 cut(s) 94, 362
PspPPI RGGWCCY 1 cut(s) 362
PstI CTGCAG 2 cut(s) 298, 675
PstNI CAGNNNCTG 2 cut(s) 293, 365
PsyI GACNNNGTC 1 cut(s) 316
PvuII CAGCTG 2 cut(s) 299, 639
RseI CAYNNNNRTG 1 cut(s) 308
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 3 cut(s) 288, 297, 671
Sau3AI GATC 1 cut(s) 4
Sau96I GGNCC 2 cut(s) 94, 362
SchI GAGTC 1 cut(s) 97
ScrFI CCNGG 2 cut(s) 133, 366
SduI GDGCHC 1 cut(s) 188
SexAI ACCWGGT 1 cut(s) 364
SfaNI GCATC 4 cut(s) 51, 89, 299, 611
SfcI CTRYAG 3 cut(s) 234, 294, 671
SfuI TTCGAA 1 cut(s) 552
SinI GGWCC 2 cut(s) 94, 362
SmiMI CAYNNNNRTG 1 cut(s) 308
SmlI CTYRAG 2 cut(s) 107, 181
SmoI CTYRAG 2 cut(s) 107, 181
SphI GCATGC 1 cut(s) 244
Sse9I AATT 6 cut(s) 36, 49, 153, 306, 331, 556
SsiI CCGC 2 cut(s) 279, 628
StyD4I CCNGG 2 cut(s) 131, 364
TaaI ACNGT 1 cut(s) 318
TaqI TCGA 3 cut(s) 145, 151, 552
TasI AATT 6 cut(s) 36, 49, 153, 306, 331, 556
TfiI GAWTC 2 cut(s) 115, 406
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TscAI CASTG 3 cut(s) 104, 321, 472
TseFI GTSAC 1 cut(s) 525
TseI GCWGC 3 cut(s) 287, 296, 670
Tsp45I GTSAC 1 cut(s) 525
TspDTI ATGAA 1 cut(s) 45
TspRI CASTG 3 cut(s) 104, 321, 472
Tth111I GACNNNGTC 1 cut(s) 316
Vha464I CTTAAG 1 cut(s) 107
VpaK11BI GGWCC 2 cut(s) 94, 362
XagI CCTNNNNNAGG 1 cut(s) 130
XapI RAATTY 3 cut(s) 49, 153, 556
XceI RCATGY 1 cut(s) 244
XmnI GAANNNNTTC 1 cut(s) 548
Zsp2I ATGCAT 2 cut(s) 82, 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.