Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0004340

Physical Location & Seq

Genomic Coordinates Forward (+)

14528624 .. 14537964

Gene Structure 9341 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0089200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3471 bp

ATGGATCCCAACGAGACCGCACAGCAGCTTCTTGCCCTTCATGCTAGAATTGTGGGAGACATGGAGTCAGAGACTATGGTCTGTACCACACATCAACGTGTTGAAGAAGAGCCACAGTACACACATATTGACCAGAATGAATTCTCACAGAGAAGGCACAAGAAGCAGGAGGAGGAGGAGATTGTCATATGTGAATGCAAGTATGATGAAAAGGATCCTGAAAGTGCATGTGGAGAAAGGTGCTTGAATGTGTTGACCAGCACGGAATGCACTCCTGGATATTGCCCTTGTGGTGTCTATTGTAAGAATCAGAGATTTCAGAAATGTGAATATGCCAAGACAAAATTGTTTAAAACAGAAGGCCGTGGTTGGGGTCTTTTAGCCAATGAGAACATTAAGGCAGGGCAGTTTATTATTGAATACTGTGGAGAAGTGATATCATGGAAGGAAGCAAAGAAAAGATCTCATGCTTATGAAGTTGAAGGTATCAGGGATGCATTTATTATTTCTCTCAATGCCTCTGAGTCTATTGATGCCACTGTGAAGGGAAGCCTAGCGAGATTTATAAATCATTCTTGCCAACCAAATTGTGAGACAAGAAAGTGGAATGTGCTTGGGGAAATTAGGGTTGGAATATTTGCAAAACAAGATATATTAGCTGGAACTGAGTTGGCATATGACTACAATTTTGAATGGTATGGGGGTGTGAAGGTTCGCTGCCTCTGTGGTGCACCCAGCTGTTCGGGATTTCTTGGGGCAAAATCTCGTGGTTTTCAGGGTGATACTTCTAGTCGAGGTAGTGGGGTTGGGTCTGATACTGCAATGGATTCCACGACTCAATCTAGTACTCATGAGGCAAACACTTCTACTCGAGGTAGTGAGGTCGACTCAGAATGTGTAATGGATTTCACAAGTGAATTTACTACTTATGAGATTTTCAAGAGTAGAGAATCCTTGATGCTGTGGGCTCGTGAGGCCGGGAAACGAAACGGGTTTGTCGTTGTGACTGTACGATCTGATTTGGGTGGTAAAAGTTTGAAACCAAGAGTTACATTAGGGTGTGAGCGTGGTGGAAAATTTAAGGTTCACAAAGAAAGATGTGATAAAGGTGGGAAGCAAAGATGTGGGACTGGCAGCAAGAAGTGTGGCTGTCCTTTCACATTAAAGGGAGAGAAGTTAACAACTGGTGATGGATGGATGCTAAAGGTAGTATGTGGAGTGCACAATCATGCCTTAACCGAGTCTCTTGAAGGGCACTCATATGCAGGGAGGTTGTCTGAGGAGGAGACTTCACTCTTGATAGACTTATCAAAGAGTTTAGTACGACCCAAAGAAATCTTGACCAAATTGAAGCAAAGGGATTCACTCAATGTTACAACTATAAGAACTATCTACAATGCACGCCAGAGGCAAAGAGTTAAAGAGAAAGCAGGGTGGTCTCAAATGGAACAACTTTTATGCAAGTTGAATGAGCATAATTATCTTGAGTGGCACAGGAGTTGCCCTATTTCAGGGAGTGTGCTGGACTTGTTTTGGGCACACCCTGTGAGCTTGGAATTGTTACGTGTATTTCCAACCATCTTGATGATGGATTGCACTTTTAAAACCAATAGGTATCGTTATCCTCTCCTAGAGGTTGTAGGTGTGACATCTACTAATATGACATTCTCAGTTGCATTTGTATTTTTGGATTCTGAAAAAGAAGATAATTATGTGTGGGCCTTGAATAGATTACGTGATGTGTTGGATGGGTGTGCTATGCCCAATGTTATTGTCACAGACAAGGATTTGGAATTGATGAGAGCCGTTGAGATGGTATTTCCTTCAGCTAGACATATGCTGTGCAAATGGCATATCAATAAGAATGTGTTGGCAAAATGCAGAAATCTGTTTGAGTCCAAGGAAAAGTGGGATAGGTTTATTATGAAGTGGAATGTGCTTGTGACGTCACCTACTGAAGAAGACTACATACAAGAGCTAGCAGTGCTACAGAGTGAATTTAGTAATTACACAGAGGTCTTGGACTATGTTACAAACACTTGGTTGAATCCTTTCAAGGATCGATTTGTGTCGGCCTGGACAGACGTAATCATGCATTTTGGTAATGTGGCATCAAACAGGGCGGAGAGTGCACATTCTAAGCTTAAGAGGCAACTTGGTTTGAGTCAGGGGAATTTTGAGGGTTCTTTTGAGAAGATACACGCTCTACTTGAGTTACAACACACTGACATTAAGGCATCTTTCGAGAAATGTTTGACTGTTGTACGACATAGCTCCTTGCCTTTAGAATTCAAACATCTAAGAGGAGTAGTTTCTACTTTTGCATTGGACAAGGTATTATCTCAGTTTAAGTTAGCCACCTCAATTGGAATTGATGATTTTCATTGTGGTTGTGTCATACGTCGCACCCATGGTTTACCTTGTGCCCATGAAATTGCCAAATATAAGAGGGATTGTCAACCAATTCCGCTCGATTCTCTTGACCCCCATTGGAGGAAACTTCATATGTCACCCTCTTCTGATCCTATTAATGATAATATGGATGTTAATGAATTTCCTGAGTTGGAGTTGCTTCAACAGTACTTTTTAAAGACAAGTGCTGATAATCGGTTGATGCTGCGGAAAAGAGTGAGAGAACTTGTACCATCCCTAGCAACTCTTATTGAACCAGGGGTGAAGTCCCGAACACGAGGTCAATTGAAGGAGAGAATTGATATGCCCATTCAGAAAAACACATCTGTGTTTGAGTTAATACAATCTGCATCTGTGGATAGTCATTCACCTAGTGTGACTGGTGGTACAATGGATGTTTTGAAAAAATCAACACATAAGCAGACAGGGCCGATGAAGGAGAAGGTTTACCGGACACTCTCAGCAAAAGTTGTGTCATATATCGATTCATTTCCAGTGGGATTGATGCCTTACATAGACCAAGTCAAGGATGTATGTGATGATGGTAACTGTGGGTTTAGAGCGGTCGCTGATTTAGTAGGATTGGGCGAGAACGCATACTTGCAAGTGAGAAATGACCTACTGACTGAATTGTCTTCCTATTCTGCACATTATAGTGAGCTATACGGCAGCGCAGAGAAGGTTCGTGACTTGGCACAATCACTTTCATTCTTCAATGAGGGTAGAGCGCCATTTGACAGATGCATGACAATGCCTGACATGGGTCATCTCATAGCATCATGCTATAAGATTGTACTCTTCCACTTTTCAGCTACTCAATGCCTTACCTTCCTACCTCTTCGATCAACACCTGTTCCTTTGCCAATGCGTAGACATATTGCTATTGGTCTTGTTAATGATTCCCATTATGTTGAGGTATTCTTAAAGCCAGGACATCCTGTACCACCCATTGAGTCTAATTGGATCAGATATCATACACAAGCTGCCCAAGGGTGGGATACTATATATACTGATTGTATTGCACACTTCCAGTTGCTTGCCCGCTCGGATAACATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005783 GO:0005789 GO:0005819 GO:0005856 GO:0006996 GO:0006997 GO:0007049 GO:0007059 GO:0007127 GO:0008104 GO:0008150 GO:0008360 GO:0009524 GO:0009987 GO:0012505 GO:0015630 GO:0016020 GO:0016043 GO:0022402 GO:0022414 GO:0022603 GO:0022604 GO:0022607 GO:0031967 GO:0031975 GO:0031984 GO:0032878 GO:0033036 GO:0033365 GO:0034397 GO:0034398 GO:0034504 GO:0034613 GO:0042175 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043495 GO:0043621 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044430 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044821 GO:0045132 GO:0045141 GO:0045143 GO:0048285 GO:0050000 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051179 GO:0051234 GO:0051259 GO:0051260 GO:0051276 GO:0051291 GO:0051303 GO:0051321 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0061982 GO:0065003 GO:0065007 GO:0065008 GO:0070192 GO:0070197 GO:0070727 GO:0071840 GO:0090220 GO:0090435 GO:0097240 GO:0098813 GO:0098827 GO:0140013 GO:1903046 GO:2000769

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sad1_UNC

Protein Analysis

1156

Amino Acids

130.94

Weight (kDa)

7.18

Isoelectric Point (pI)

42.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AWS	PF17907	72 - 109	3.9e-14	AWS domain
SET	PF00856	123 - 229	5.6e-25	SET domain
ZSWIM1-3_RNaseH-like	PF21056	519 - 612	9.8e-12	Zinc finger SWIM domain-containing protein 1/3, RNaseH-like domain
MULE	PF10551	528 - 622	2.6e-26	MULE transposase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004340)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10730 AT5G04990
fragaria_vesca	FvH4_7g04870
malus_domestica	MD02G1266700.v1.1 MD07G1053000.v1.1
prunus_persica	Prupe.2G056900_v2.0.a1
pyrus_communis	pycom02g22820 pycom07g04100
rosa_chinensis	RchiOBHm_Chr1g0324281 RchiOBHm_Chr1g0330551
rosa_laevigata	RLG00000029806 RLG00000030209
rosa_multiflora	Rmu_co8045364.1_g000001 Rmu_co8316659.1_g000001 Rmu_co8464795.1_g000001 Rmu_sc0001850.1_g000044 Rmu_sc0002365.1_g000026 Rmu_sc0007290.1_g000001 Rmu_sc0037448.1_g000001
rosa_roxburghii	Rroxscaffold_159G00432660 Rroxscaffold_4G00319920
rosa_rugosa	Rorug01G0089200 Rorug02G0533700
rosa_samantha	Rh1AG060500 Rh1AG107300 Rh1BG086100 Rh1CG103200 Rh1DG067200 Rh1DG112900
rosa_wichuraiana	Rw1G005160 Rw1G008530 Rw1G008910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	566
AasI	GACNNNNNNGTC	1 cut(s)	3043
AatII	GACGTC	1 cut(s)	1949
AccBSI	CCGCTC	3 cut(s)	2470, 2975, 3456
AccI	GTMKAC	2 cut(s)	885, 3283
AciI	CCGC	6 cut(s)	18, 2123, 2468, 2620, 2975, 3454
AclWI	GGATC	6 cut(s)	12, 209, 222, 2067, 2516, 3383
AcsI	RAATTY	7 cut(s)	140, 917, 1076, 1997, 2173, 2288, 2552
AcuI	CTGAAG	2 cut(s)	1809, 1977
AcyI	GRCGYC	1 cut(s)	1946
AdeI	CACNNNGTG	2 cut(s)	1546, 2786
AfiI	CCNNNNNNNGG	7 cut(s)	370, 1511, 2493, 2938, 3173, 3405, 3406
AflII	CTTAAG	1 cut(s)	2144
AflIII	ACRYGT	2 cut(s)	97, 1564
AhdI	GACNNNNNGTC	1 cut(s)	64
AjnI	CCWGG	4 cut(s)	274, 2075, 2668, 3340
AjuI	GAANNNNNNNTTGG	2 cut(s)	612, 644
AleI	CACNNNNGTG	1 cut(s)	2738
Alw21I	GWGCWC	3 cut(s)	733, 1224, 2134
Alw26I	GTCTC	7 cut(s)	8, 51, 65, 587, 1248, 1280, 1443
Alw44I	GTGCAC	3 cut(s)	729, 1220, 2130
AlwI	GGATC	6 cut(s)	12, 209, 222, 2067, 2516, 3383
Ama87I	CYCGRG	1 cut(s)	870
AoxI	GGCC	5 cut(s)	361, 975, 1719, 2073, 2840
ApaLI	GTGCAC	3 cut(s)	729, 1220, 2130
ApeKI	GCWGC	6 cut(s)	25, 717, 1134, 2617, 3081, 3395
ApoI	RAATTY	7 cut(s)	140, 917, 1076, 1997, 2173, 2288, 2552
ArsI	GACNNNNNNTTYG	6 cut(s)	671, 703, 2677, 2709, 2870, 2902
AseI	ATTAAT	1 cut(s)	2529
Asp700I	GAANNNNTTC	2 cut(s)	140, 2051
AspLEI	GCGC	2 cut(s)	3086, 3142
AspS9I	GGNCC	2 cut(s)	1719, 2840
AsuC2I	CCSGG	1 cut(s)	979
AsuHPI	GGTGA	6 cut(s)	791, 1199, 1941, 2502, 2686, 2772
AsuNHI	GCTAGC	1 cut(s)	1978
AvaI	CYCGRG	1 cut(s)	870
BaeGI	GKGCMC	6 cut(s)	733, 1224, 1257, 1540, 2134, 2428
BamHI	GGATCC	2 cut(s)	4, 214
BanII	GRGCYC	1 cut(s)	970
BarI	GAAGNNNNNNTAC	2 cut(s)	1953, 1985
BauI	CACGAG	3 cut(s)	765, 969, 2688
BbsI	GAAGAC	2 cut(s)	1968, 3039
Bbv12I	GWGCWC	3 cut(s)	733, 1224, 2134
BbvI	GCAGC	6 cut(s)	37, 704, 1146, 2604, 3093, 3382
BccI	CCATC	8 cut(s)	1184, 1188, 1582, 1586, 1742, 1807, 2653, 2948
BceAI	ACGGC	3 cut(s)	348, 1790, 3094
BciT130I	CCWGG	4 cut(s)	276, 2077, 2670, 3342
BciVI	GTATCC	1 cut(s)	3403
BcnI	CCSGG	1 cut(s)	979
BcoDI	GTCTC	7 cut(s)	8, 51, 65, 587, 1248, 1280, 1443
BfaI	CTAG	9 cut(s)	45, 554, 789, 843, 1631, 1830, 1979, 2651, 2784
BfmI	CTRYAG	1 cut(s)	1988
BfoI	RGCGCY	1 cut(s)	3143
BfrI	CTTAAG	1 cut(s)	2144
BfuI	GTATCC	1 cut(s)	3403
BglII	AGATCT	1 cut(s)	461
BisI	GCNGC	6 cut(s)	26, 718, 1135, 2618, 3082, 3396
BlsI	GCNGC	6 cut(s)	27, 719, 1136, 2619, 3083, 3397
BmcAI	AGTACT	2 cut(s)	847, 2582
Bme1390I	CCNGG	5 cut(s)	276, 979, 2077, 2670, 3342
BmeRI	GACNNNNNGTC	1 cut(s)	64
BmeT110I	CYCGRG	1 cut(s)	870
BmgT120I	GGNCC	2 cut(s)	1719, 2840
BmiI	GGNNCC	2 cut(s)	6, 216
BmrFI	CCNGG	5 cut(s)	276, 979, 2077, 2670, 3342
BmtI	GCTAGC	1 cut(s)	1982
BoxI	GACNNNNGTC	2 cut(s)	77, 3174
BpiI	GAAGAC	2 cut(s)	1968, 3039
BplI	GAGNNNNNCTC	2 cut(s)	872, 904
BpuEI	CTTGAG	2 cut(s)	1505, 2231
BpuMI	CCSGG	1 cut(s)	979
Bsa29I	ATCGAT	2 cut(s)	2062, 2895
BsaAI	YACGTR	2 cut(s)	1565, 1736
BsaHI	GRCGYC	1 cut(s)	1946
BsaI	GGTCTC	2 cut(s)	8, 1443
BsaJI	CCNNGG	5 cut(s)	364, 1899, 2410, 2669, 3400
BsaWI	WCCGGW	1 cut(s)	2862
Bsc4I	CCNNNNNNNGG	7 cut(s)	370, 1511, 2493, 2938, 3173, 3405, 3406
Bse1I	ACTGG	5 cut(s)	1135, 1189, 2797, 2906, 3442
Bse3DI	GCAATG	1 cut(s)	828
BseBI	CCWGG	4 cut(s)	276, 2077, 2670, 3342
BseCI	ATCGAT	2 cut(s)	2062, 2895
BseDI	CCNNGG	5 cut(s)	364, 1899, 2410, 2669, 3400
BseGI	GGATG	9 cut(s)	499, 1199, 1203, 1753, 2548, 2645, 2812, 2947, 3346
BseLI	CCNNNNNNNGG	7 cut(s)	370, 1511, 2493, 2938, 3173, 3405, 3406
BseMI	GCAATG	1 cut(s)	828
BseMII	CTCAG	8 cut(s)	513, 657, 903, 1269, 1683, 2357, 2550, 2886
BseNI	ACTGG	5 cut(s)	1135, 1189, 2797, 2906, 3442
BseRI	GAGGAG	6 cut(s)	185, 188, 191, 1295, 1298, 2319
BseSI	GKGCMC	6 cut(s)	733, 1224, 1257, 1540, 2134, 2428
BseXI	GCAGC	6 cut(s)	37, 704, 1146, 2604, 3093, 3382
BseYI	CCCAGC	1 cut(s)	734
BsgI	GTGCAG	1 cut(s)	3042
Bsh1285I	CGRYCG	1 cut(s)	2979
BshFI	GGCC	5 cut(s)	363, 977, 1721, 2075, 2842
BshVI	ATCGAT	2 cut(s)	2062, 2895
BsiEI	CGRYCG	1 cut(s)	2979
BsiHKAI	GWGCWC	3 cut(s)	733, 1224, 2134
BsiHKCI	CYCGRG	1 cut(s)	870
BsiSI	CCGG	2 cut(s)	978, 2863
BslFI	GGGAC	2 cut(s)	1141, 2665
BslI	CCNNNNNNNGG	7 cut(s)	370, 1511, 2493, 2938, 3173, 3405, 3406
BsmAI	GTCTC	7 cut(s)	8, 51, 65, 587, 1248, 1280, 1443
BsmFI	GGGAC	2 cut(s)	1141, 2665
BsmI	GAATGC	2 cut(s)	200, 272
BsnI	GGCC	5 cut(s)	363, 977, 1721, 2075, 2842
Bso31I	GGTCTC	2 cut(s)	8, 1443
BsoBI	CYCGRG	1 cut(s)	870
Bsp1286I	GDGCHC	7 cut(s)	733, 970, 1224, 1257, 1540, 2134, 2428
Bsp143I	GATC	8 cut(s)	4, 214, 461, 1013, 2059, 2521, 3254, 3375
Bsp19I	CCATGG	1 cut(s)	2410
BspACI	CCGC	6 cut(s)	18, 2123, 2468, 2620, 2975, 3454
BspANI	GGCC	5 cut(s)	363, 977, 1721, 2075, 2842
BspCNI	CTCAG	8 cut(s)	514, 658, 902, 1270, 1682, 2356, 2551, 2885
BspDI	ATCGAT	2 cut(s)	2062, 2895
BspHI	TCATGA	1 cut(s)	850
BspLI	GGNNCC	2 cut(s)	6, 216
BspOI	GCTAGC	1 cut(s)	1982
BspPI	GGATC	6 cut(s)	12, 209, 222, 2067, 2516, 3383
BspQI	GCTCTTC	1 cut(s)	102
BspTI	CTTAAG	1 cut(s)	2144
BspTNI	GGTCTC	2 cut(s)	8, 1443
BsrBI	CCGCTC	3 cut(s)	2470, 2975, 3456
BsrDI	GCAATG	1 cut(s)	828
BsrI	ACTGG	5 cut(s)	1135, 1189, 2797, 2906, 3442
BssECI	CCNNGG	5 cut(s)	364, 1899, 2410, 2669, 3400
BssMI	GATC	8 cut(s)	4, 214, 461, 1013, 2059, 2521, 3254, 3375
BssNI	GRCGYC	1 cut(s)	1946
BssSI	CACGAG	3 cut(s)	765, 969, 2688
BssT1I	CCWWGG	3 cut(s)	1899, 2410, 3400
Bst2BI	CACGAG	3 cut(s)	765, 969, 2688
Bst2UI	CCWGG	4 cut(s)	276, 2077, 2670, 3342
Bst4CI	ACNGT	7 cut(s)	117, 425, 541, 1009, 2260, 2580, 2963
Bst6I	CTCTTC	4 cut(s)	102, 2521, 3215, 3255
BstACI	GRCGYC	1 cut(s)	1946
BstAFI	CTTAAG	1 cut(s)	2144
BstAPI	GCANNNNNTGC	1 cut(s)	267
BstBAI	YACGTR	2 cut(s)	1565, 1736
BstC8I	GCNNGC	4 cut(s)	1402, 1980, 3450, 3454
BstDSI	CCRYGG	2 cut(s)	364, 2410
BstENI	CCTNNNNNAGG	1 cut(s)	1509
BstF5I	GGATG	9 cut(s)	499, 1199, 1203, 1753, 2548, 2645, 2812, 2947, 3346
BstH2I	RGCGCY	1 cut(s)	3143
BstHHI	GCGC	2 cut(s)	3086, 3142
BstKTI	GATC	8 cut(s)	7, 217, 464, 1016, 2062, 2524, 3257, 3378
BstMAI	GTCTC	7 cut(s)	8, 51, 65, 587, 1248, 1280, 1443
BstMBI	GATC	8 cut(s)	4, 214, 461, 1013, 2059, 2521, 3254, 3375
BstMCI	CGRYCG	1 cut(s)	2979
BstMWI	GCNNNNNNNGC	8 cut(s)	41, 163, 267, 974, 1984, 2129, 2149, 2839
BstNI	CCWGG	4 cut(s)	276, 2077, 2670, 3342
BstNSI	RCATGY	1 cut(s)	231
BstPAI	GACNNNNGTC	2 cut(s)	77, 3174
BstSCI	CCNGG	5 cut(s)	274, 977, 2075, 2668, 3340
BstSFI	CTRYAG	1 cut(s)	1988
BstSLI	GKGCMC	6 cut(s)	733, 1224, 1257, 1540, 2134, 2428
BstV1I	GCAGC	6 cut(s)	37, 704, 1146, 2604, 3093, 3382
BstV2I	GAAGAC	2 cut(s)	1968, 3039
BstX2I	RGATCY	3 cut(s)	4, 214, 461
BstYI	RGATCY	3 cut(s)	4, 214, 461
Bsu15I	ATCGAT	2 cut(s)	2062, 2895
BsuI	GTATCC	1 cut(s)	3403
BsuRI	GGCC	5 cut(s)	363, 977, 1721, 2075, 2842
BsuTUI	ATCGAT	2 cut(s)	2062, 2895
BtgI	CCRYGG	2 cut(s)	364, 2410
BtsCI	GGATG	9 cut(s)	499, 1199, 1203, 1753, 2548, 2645, 2812, 2947, 3346
BtsI	GCAGTG	1 cut(s)	1989
BtsIMutI	CAGTG	4 cut(s)	537, 1989, 2223, 2913
Cac8I	GCNNGC	4 cut(s)	1402, 1980, 3450, 3454
CciI	TCATGA	1 cut(s)	850
CfoI	GCGC	2 cut(s)	3086, 3142
Cfr13I	GGNCC	2 cut(s)	1719, 2840
ClaI	ATCGAT	2 cut(s)	2062, 2895
CspCI	CAANNNNNGTGG	4 cut(s)	1126, 1161, 2347, 2382
DpnI	GATC	8 cut(s)	6, 216, 463, 1015, 2061, 2523, 3256, 3377
DpnII	GATC	8 cut(s)	4, 214, 461, 1013, 2059, 2521, 3254, 3375
DraI	TTTAAA	3 cut(s)	352, 1603, 2589
DraIII	CACNNNGTG	2 cut(s)	1546, 2786
DrdI	GACNNNNNNGTC	1 cut(s)	3043
DriI	GACNNNNNGTC	1 cut(s)	64
DseDI	GACNNNNNNGTC	1 cut(s)	3043
Eam1104I	CTCTTC	4 cut(s)	102, 2521, 3215, 3255
Eam1105I	GACNNNNNGTC	1 cut(s)	64
EarI	CTCTTC	4 cut(s)	102, 2521, 3215, 3255
EciI	GGCGGA	1 cut(s)	2138
Eco130I	CCWWGG	3 cut(s)	1899, 2410, 3400
Eco24I	GRGCYC	1 cut(s)	970
Eco31I	GGTCTC	2 cut(s)	8, 1443
Eco32I	GATATC	2 cut(s)	438, 3383
Eco57I	CTGAAG	2 cut(s)	1809, 1977
Eco88I	CYCGRG	1 cut(s)	870
EcoNI	CCTNNNNNAGG	1 cut(s)	1509
EcoRI	GAATTC	2 cut(s)	140, 2288
EcoRII	CCWGG	4 cut(s)	274, 2075, 2668, 3340
EcoRV	GATATC	2 cut(s)	438, 3383
EcoT14I	CCWWGG	3 cut(s)	1899, 2410, 3400
EcoT22I	ATGCAT	3 cut(s)	499, 2097, 3158
EcoT38I	GRGCYC	1 cut(s)	970
ErhI	CCWWGG	3 cut(s)	1899, 2410, 3400
FaqI	GGGAC	2 cut(s)	1141, 2665
FauI	CCCGC	1 cut(s)	3461
FauNDI	CATATG	5 cut(s)	188, 676, 1261, 1836, 2505
FblI	GTMKAC	2 cut(s)	885, 3283
Fnu4HI	GCNGC	6 cut(s)	26, 718, 1135, 2618, 3082, 3396
FokI	GGATG	9 cut(s)	506, 1206, 1210, 1760, 2555, 2632, 2819, 2954, 3333
FriOI	GRGCYC	1 cut(s)	970
Fsp4HI	GCNGC	6 cut(s)	26, 718, 1135, 2618, 3082, 3396
FspBI	CTAG	9 cut(s)	45, 554, 789, 843, 1631, 1830, 1979, 2651, 2784
GlaI	GCGC	2 cut(s)	3085, 3141
GluI	GCNGC	6 cut(s)	26, 718, 1135, 2618, 3082, 3396
GsaI	CCCAGC	1 cut(s)	738
HaeII	RGCGCY	1 cut(s)	3143
HaeIII	GGCC	5 cut(s)	363, 977, 1721, 2075, 2842
HapII	CCGG	2 cut(s)	978, 2863
HhaI	GCGC	2 cut(s)	3086, 3142
Hin1I	GRCGYC	1 cut(s)	1946
Hin6I	GCGC	2 cut(s)	3084, 3140
HinP1I	GCGC	2 cut(s)	3084, 3140
HincII	GTYRAC	4 cut(s)	255, 886, 1179, 2459
HindII	GTYRAC	4 cut(s)	255, 886, 1179, 2459
HindIII	AAGCTT	1 cut(s)	2141
HpaI	GTTAAC	1 cut(s)	1179
HpaII	CCGG	2 cut(s)	978, 2863
HphI	GGTGA	6 cut(s)	791, 1199, 1941, 2502, 2686, 2772
Hpy99I	CGWCG	1 cut(s)	2406
HpyCH4III	ACNGT	7 cut(s)	117, 425, 541, 1009, 2260, 2580, 2963
HpyCH4IV	ACGT	6 cut(s)	97, 1564, 1735, 1946, 2085, 2401
HpyF10VI	GCNNNNNNNGC	8 cut(s)	41, 163, 267, 974, 1984, 2129, 2149, 2839
HpySE526I	ACGT	6 cut(s)	97, 1564, 1735, 1946, 2085, 2401
Hsp92I	GRCGYC	1 cut(s)	1946
HspAI	GCGC	2 cut(s)	3084, 3140
KspAI	GTTAAC	1 cut(s)	1179
Kzo9I	GATC	8 cut(s)	4, 214, 461, 1013, 2059, 2521, 3254, 3375
LguI	GCTCTTC	1 cut(s)	102
LmnI	GCTCC	1 cut(s)	2279
Lsp1109I	GCAGC	6 cut(s)	37, 704, 1146, 2604, 3093, 3382
MaeI	CTAG	9 cut(s)	45, 554, 789, 843, 1631, 1830, 1979, 2651, 2784
MaeII	ACGT	6 cut(s)	97, 1564, 1735, 1946, 2085, 2401
MalI	GATC	8 cut(s)	6, 216, 463, 1015, 2061, 2523, 3256, 3377
MbiI	CCGCTC	3 cut(s)	2470, 2975, 3456
MboI	GATC	8 cut(s)	4, 214, 461, 1013, 2059, 2521, 3254, 3375
MfeI	CAATTG	2 cut(s)	2364, 2696
MflI	RGATCY	3 cut(s)	4, 214, 461
MhlI	GDGCHC	7 cut(s)	733, 970, 1224, 1257, 1540, 2134, 2428
MlyI	GAGTC	8 cut(s)	74, 533, 829, 881, 1250, 1904, 2173, 3374
MmeI	TCCRAC	4 cut(s)	610, 1598, 1725, 2544
Mph1103I	ATGCAT	3 cut(s)	499, 2097, 3158
MroXI	GAANNNNTTC	2 cut(s)	140, 2051
MslI	CAYNNNNRTG	9 cut(s)	96, 471, 1057, 1227, 1260, 1583, 2738, 3066, 3161
MspA1I	CMGCKG	1 cut(s)	738
MspCI	CTTAAG	1 cut(s)	2144
MspI	CCGG	2 cut(s)	978, 2863
MspR9I	CCNGG	5 cut(s)	276, 979, 2077, 2670, 3342
MunI	CAATTG	2 cut(s)	2364, 2696
Mva1269I	GAATGC	2 cut(s)	200, 272
MvaI	CCWGG	4 cut(s)	276, 2077, 2670, 3342
MwoI	GCNNNNNNNGC	8 cut(s)	41, 163, 267, 974, 1984, 2129, 2149, 2839
NciI	CCSGG	1 cut(s)	979
NcoI	CCATGG	1 cut(s)	2410
NdeI	CATATG	5 cut(s)	188, 676, 1261, 1836, 2505
NdeII	GATC	8 cut(s)	4, 214, 461, 1013, 2059, 2521, 3254, 3375
NheI	GCTAGC	1 cut(s)	1978
NlaIV	GGNNCC	2 cut(s)	6, 216
NmuCI	GTSAC	8 cut(s)	1003, 1645, 1774, 1942, 1947, 2508, 2788, 3098
NsiI	ATGCAT	3 cut(s)	499, 2097, 3158
NspI	RCATGY	1 cut(s)	231
OliI	CACNNNNGTG	1 cut(s)	2738
PaeR7I	CTCGAG	1 cut(s)	870
PagI	TCATGA	1 cut(s)	850
PciSI	GCTCTTC	1 cut(s)	102
PcsI	WCGNNNNNNNCGW	1 cut(s)	996
PctI	GAATGC	2 cut(s)	200, 272
PdmI	GAANNNNTTC	2 cut(s)	140, 2051
PfeI	GAWTC	9 cut(s)	307, 827, 950, 1361, 1691, 2047, 2474, 2897, 3311
PflFI	GACNNNGTC	1 cut(s)	2933
PfoI	TCCNGGA	1 cut(s)	274
PkrI	GCNGC	6 cut(s)	27, 719, 1136, 2619, 3083, 3397
PleI	GAGTC	8 cut(s)	73, 532, 829, 881, 1249, 1903, 2172, 3373
PpsI	GAGTC	8 cut(s)	73, 532, 829, 881, 1249, 1903, 2172, 3373
Ppu21I	YACGTR	2 cut(s)	1565, 1736
PshAI	GACNNNNGTC	2 cut(s)	77, 3174
PshBI	ATTAAT	1 cut(s)	2529
PsiI	TTATAA	1 cut(s)	566
Psp6I	CCWGG	4 cut(s)	274, 2075, 2668, 3340
PspFI	CCCAGC	1 cut(s)	734
PspGI	CCWGG	4 cut(s)	274, 2075, 2668, 3340
PspN4I	GGNNCC	2 cut(s)	6, 216
PspPI	GGNCC	2 cut(s)	1719, 2840
PspXI	VCTCGAGB	1 cut(s)	870
PsuI	RGATCY	3 cut(s)	4, 214, 461
PsyI	GACNNNGTC	1 cut(s)	2933
PvuII	CAGCTG	1 cut(s)	738
RseI	CAYNNNNRTG	9 cut(s)	96, 471, 1057, 1227, 1260, 1583, 2738, 3066, 3161
SalI	GTCGAC	1 cut(s)	884
SapI	GCTCTTC	1 cut(s)	102
SatI	GCNGC	6 cut(s)	26, 718, 1135, 2618, 3082, 3396
Sau3AI	GATC	8 cut(s)	4, 214, 461, 1013, 2059, 2521, 3254, 3375
Sau96I	GGNCC	2 cut(s)	1719, 2840
ScaI	AGTACT	2 cut(s)	847, 2582
SchI	GAGTC	8 cut(s)	74, 533, 829, 881, 1250, 1904, 2173, 3374
ScrFI	CCNGG	5 cut(s)	276, 979, 2077, 2670, 3342
SduI	GDGCHC	7 cut(s)	733, 970, 1224, 1257, 1540, 2134, 2428
SfcI	CTRYAG	1 cut(s)	1988
Sfr274I	CTCGAG	1 cut(s)	870
SlaI	CTCGAG	1 cut(s)	870
SmiMI	CAYNNNNRTG	9 cut(s)	96, 471, 1057, 1227, 1260, 1583, 2738, 3066, 3161
SmlI	CTYRAG	4 cut(s)	870, 1484, 2144, 2210
SmoI	CTYRAG	4 cut(s)	870, 1484, 2144, 2210
SsiI	CCGC	6 cut(s)	18, 2123, 2468, 2620, 2975, 3454
SspI	AATATT	1 cut(s)	636
SspMI	CTAG	9 cut(s)	45, 554, 789, 843, 1631, 1830, 1979, 2651, 2784
StyD4I	CCNGG	5 cut(s)	274, 977, 2075, 2668, 3340
StyI	CCWWGG	3 cut(s)	1899, 2410, 3400
TaaI	ACNGT	7 cut(s)	117, 425, 541, 1009, 2260, 2580, 2963
TaiI	ACGT	6 cut(s)	100, 1567, 1738, 1949, 2088, 2404
TaqI	TCGA	8 cut(s)	793, 871, 885, 2062, 2244, 2472, 2895, 3253
TatI	WGTACW	4 cut(s)	117, 845, 2580, 3205
TfiI	GAWTC	9 cut(s)	307, 827, 950, 1361, 1691, 2047, 2474, 2897, 3311
TscAI	CASTG	4 cut(s)	544, 1989, 2230, 2913
TseFI	GTSAC	8 cut(s)	1003, 1645, 1774, 1942, 1947, 2508, 2788, 3098
TseI	GCWGC	6 cut(s)	25, 717, 1134, 2617, 3081, 3395
Tsp45I	GTSAC	8 cut(s)	1003, 1645, 1774, 1942, 1947, 2508, 2788, 3098
TspGWI	ACGGA	1 cut(s)	278
TspRI	CASTG	4 cut(s)	544, 1989, 2230, 2913
Tth111I	GACNNNGTC	1 cut(s)	2933
Vha464I	CTTAAG	1 cut(s)	2144
VneI	GTGCAC	3 cut(s)	729, 1220, 2130
VspI	ATTAAT	1 cut(s)	2529
XagI	CCTNNNNNAGG	1 cut(s)	1509
XapI	RAATTY	7 cut(s)	140, 917, 1076, 1997, 2173, 2288, 2552
XceI	RCATGY	1 cut(s)	231
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1585
XhoI	CTCGAG	1 cut(s)	870
XmiI	GTMKAC	2 cut(s)	885, 3283
XmnI	GAANNNNTTC	2 cut(s)	140, 2051
XspI	CTAG	9 cut(s)	45, 554, 789, 843, 1631, 1830, 1979, 2651, 2784
ZraI	GACGTC	1 cut(s)	1947
ZrmI	AGTACT	2 cut(s)	847, 2582
Zsp2I	ATGCAT	3 cut(s)	499, 2097, 3158

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.