Rorug01G0122600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0005542
Physical Location & Seq
Reverse (-)
21207089 .. 21207649
561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0122600.1

Sequence Viewer

Length: 561 bp
ATGGGCAATCTCCTAGCATCTACGTCCTCTGGTGATCCTTATCATGGTCTCTGGAAGAAGGTTTCGAACACAAAAGTTCCTGGCAAGGTTAAAATATGTATATGGAGGGTTGCAAACAATATTCTACCTACTCGTGAAAGCCTAAGTCACAAGGGATATTCAGGCTCGTTGCATTTCTTGCTATGCAATGAAACCTGGGAAACAACCAGCCATGTTTTTTGTCACTGTTCTGTTGCCCAAGAAACCATTGCAATTGCTAATATTCTGATCACAGATTGCTTACCTTGCAGCTTCAAAGATTGGTTGATACTTCAGAGCCAACATCTTACTCGAGAGAATTGGGAGCGACTTATTATGCTTCTATGGGCCTTGTGGAAGAACAGAAATGGTAAGTTATGGAATGAAATAAACAAGAGTGCACATGAGATCGTTGTTGCTATTGCTACATGGTATCAACAGTTCTTGCAAGCACAACAAAGGCCAACACAAGACGAAGCAGCTCATCCAGGTAAATATTGGACTTCACCAGTAACACAACAGCTAGCTCAAAATCATTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

21.32

Weight (kDa)

8.7

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 17 - 81 8.6e-13 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 29
AcuI CTGAAG 1 cut(s) 296
AfiI CCNNNNNNNGG 1 cut(s) 44
AgsI TTSAA 1 cut(s) 295
AjnI CCWGG 3 cut(s) 79, 194, 505
AluBI AGCT 4 cut(s) 291, 500, 541, 545
AluI AGCT 4 cut(s) 291, 500, 541, 545
Alw21I GWGCWC 1 cut(s) 421
Alw26I GTCTC 1 cut(s) 53
Alw44I GTGCAC 1 cut(s) 417
AlwI GGATC 1 cut(s) 29
Ama87I CYCGRG 1 cut(s) 330
AoxI GGCC 2 cut(s) 366, 479
ApaLI GTGCAC 1 cut(s) 417
ApeKI GCWGC 2 cut(s) 288, 497
AspS9I GGNCC 1 cut(s) 366
AsuHPI GGTGA 2 cut(s) 44, 516
AsuII TTCGAA 1 cut(s) 65
AsuNHI GCTAGC 1 cut(s) 541
AvaI CYCGRG 1 cut(s) 330
BaeGI GKGCMC 1 cut(s) 421
BauI CACGAG 1 cut(s) 132
Bbv12I GWGCWC 1 cut(s) 421
BbvI GCAGC 2 cut(s) 300, 509
BciT130I CCWGG 3 cut(s) 81, 196, 507
BclI TGATCA 1 cut(s) 267
BcoDI GTCTC 1 cut(s) 53
BfaI CTAG 2 cut(s) 14, 542
BisI GCNGC 2 cut(s) 289, 498
BlsI GCNGC 2 cut(s) 290, 499
Bme1390I CCNGG 3 cut(s) 81, 196, 507
BmeT110I CYCGRG 1 cut(s) 330
BmgT120I GGNCC 1 cut(s) 366
BmrFI CCNGG 3 cut(s) 81, 196, 507
BmsI GCATC 1 cut(s) 26
BmtI GCTAGC 1 cut(s) 545
Bpu14I TTCGAA 1 cut(s) 65
BsaBI GATNNNNATC 1 cut(s) 39
BsaI GGTCTC 1 cut(s) 53
BsaJI CCNNGG 1 cut(s) 195
Bsc4I CCNNNNNNNGG 1 cut(s) 44
Bse1I ACTGG 1 cut(s) 527
Bse3DI GCAATG 2 cut(s) 193, 246
Bse8I GATNNNNATC 1 cut(s) 39
BseBI CCWGG 3 cut(s) 81, 196, 507
BseDI CCNNGG 1 cut(s) 195
BseGI GGATG 1 cut(s) 502
BseJI GATNNNNATC 1 cut(s) 39
BseLI CCNNNNNNNGG 1 cut(s) 44
BseMI GCAATG 2 cut(s) 193, 246
BseNI ACTGG 1 cut(s) 527
BseSI GKGCMC 1 cut(s) 421
BseXI GCAGC 2 cut(s) 300, 509
BshFI GGCC 2 cut(s) 368, 481
BsiHKAI GWGCWC 1 cut(s) 421
BsiHKCI CYCGRG 1 cut(s) 330
BslI CCNNNNNNNGG 1 cut(s) 44
BsmAI GTCTC 1 cut(s) 53
BsnI GGCC 2 cut(s) 368, 481
Bso31I GGTCTC 1 cut(s) 53
BsoBI CYCGRG 1 cut(s) 330
Bsp119I TTCGAA 1 cut(s) 65
Bsp1286I GDGCHC 1 cut(s) 421
Bsp143I GATC 3 cut(s) 34, 267, 426
BspANI GGCC 2 cut(s) 368, 481
BspOI GCTAGC 1 cut(s) 545
BspPI GGATC 1 cut(s) 29
BspT104I TTCGAA 1 cut(s) 65
BspTNI GGTCTC 1 cut(s) 53
BsrDI GCAATG 2 cut(s) 193, 246
BsrI ACTGG 1 cut(s) 527
BssECI CCNNGG 1 cut(s) 195
BssMI GATC 3 cut(s) 34, 267, 426
BssSI CACGAG 1 cut(s) 132
Bst2BI CACGAG 1 cut(s) 132
Bst2UI CCWGG 3 cut(s) 81, 196, 507
Bst4CI ACNGT 2 cut(s) 227, 459
BstAPI GCANNNNNTGC 1 cut(s) 178
BstBI TTCGAA 1 cut(s) 65
BstC8I GCNNGC 2 cut(s) 468, 543
BstDEI CTNAG 1 cut(s) 143
BstF5I GGATG 1 cut(s) 502
BstKTI GATC 3 cut(s) 37, 270, 429
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 3 cut(s) 34, 267, 426
BstMWI GCNNNNNNNGC 2 cut(s) 178, 285
BstNI CCWGG 3 cut(s) 81, 196, 507
BstSCI CCNGG 3 cut(s) 79, 194, 505
BstSLI GKGCMC 1 cut(s) 421
BstV1I GCAGC 2 cut(s) 300, 509
BsuRI GGCC 2 cut(s) 368, 481
BtsCI GGATG 1 cut(s) 502
BtsIMutI CAGTG 1 cut(s) 223
Cac8I GCNNGC 2 cut(s) 468, 543
Cfr13I GGNCC 1 cut(s) 366
CviAII CATG 4 cut(s) 44, 212, 422, 447
DdeI CTNAG 1 cut(s) 143
DpnI GATC 3 cut(s) 36, 269, 428
DpnII GATC 3 cut(s) 34, 267, 426
Eco31I GGTCTC 1 cut(s) 53
Eco57I CTGAAG 1 cut(s) 296
Eco88I CYCGRG 1 cut(s) 330
EcoRII CCWGG 3 cut(s) 79, 194, 505
FaeI CATG 4 cut(s) 47, 215, 425, 450
FatI CATG 4 cut(s) 43, 211, 421, 446
FbaI TGATCA 1 cut(s) 267
Fnu4HI GCNGC 2 cut(s) 289, 498
FokI GGATG 1 cut(s) 489
Fsp4HI GCNGC 2 cut(s) 289, 498
FspBI CTAG 2 cut(s) 14, 542
GluI GCNGC 2 cut(s) 289, 498
HaeIII GGCC 2 cut(s) 368, 481
Hin1II CATG 4 cut(s) 47, 215, 425, 450
HphI GGTGA 2 cut(s) 44, 516
Hpy166II GTNNAC 1 cut(s) 419
Hpy188I TCNGA 2 cut(s) 267, 315
Hpy188III TCNNGA 3 cut(s) 52, 134, 332
Hpy8I GTNNAC 1 cut(s) 419
HpyAV CCTTC 1 cut(s) 52
HpyCH4III ACNGT 2 cut(s) 227, 459
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 7 cut(s) 113, 172, 186, 251, 288, 419, 466
HpyF10VI GCNNNNNNNGC 2 cut(s) 178, 285
HpyF3I CTNAG 1 cut(s) 143
HpySE526I ACGT 1 cut(s) 23
Hsp92II CATG 4 cut(s) 47, 215, 425, 450
Ksp22I TGATCA 1 cut(s) 267
Kzo9I GATC 3 cut(s) 34, 267, 426
LmnI GCTCC 1 cut(s) 343
Lsp1109I GCAGC 2 cut(s) 300, 509
LweI GCATC 1 cut(s) 26
MaeI CTAG 2 cut(s) 14, 542
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 3 cut(s) 146, 221, 529
MalI GATC 3 cut(s) 36, 269, 428
MboI GATC 3 cut(s) 34, 267, 426
MboII GAAGA 2 cut(s) 67, 388
MfeI CAATTG 1 cut(s) 252
MhlI GDGCHC 1 cut(s) 421
MluCI AATT 2 cut(s) 252, 337
MnlI CCTC 2 cut(s) 37, 99
MseI TTAA 2 cut(s) 90, 559
MspR9I CCNGG 3 cut(s) 81, 196, 507
MunI CAATTG 1 cut(s) 252
MvaI CCWGG 3 cut(s) 81, 196, 507
MwoI GCNNNNNNNGC 2 cut(s) 178, 285
NdeII GATC 3 cut(s) 34, 267, 426
NheI GCTAGC 1 cut(s) 541
NlaIII CATG 4 cut(s) 47, 215, 425, 450
NmuCI GTSAC 2 cut(s) 146, 221
NspV TTCGAA 1 cut(s) 65
PaeR7I CTCGAG 1 cut(s) 330
PkrI GCNGC 2 cut(s) 290, 499
Psp6I CCWGG 3 cut(s) 79, 194, 505
PspGI CCWGG 3 cut(s) 79, 194, 505
PspPI GGNCC 1 cut(s) 366
PsrI GAACNNNNNNTAC 2 cut(s) 443, 475
SaqAI TTAA 2 cut(s) 90, 559
SatI GCNGC 2 cut(s) 289, 498
Sau3AI GATC 3 cut(s) 34, 267, 426
Sau96I GGNCC 1 cut(s) 366
ScrFI CCNGG 3 cut(s) 81, 196, 507
SduI GDGCHC 1 cut(s) 421
SfaNI GCATC 1 cut(s) 26
Sfr274I CTCGAG 1 cut(s) 330
SfuI TTCGAA 1 cut(s) 65
SlaI CTCGAG 1 cut(s) 330
SmlI CTYRAG 1 cut(s) 330
SmoI CTYRAG 1 cut(s) 330
Sse9I AATT 2 cut(s) 252, 337
SspI AATATT 3 cut(s) 121, 262, 515
SspMI CTAG 2 cut(s) 14, 542
StyD4I CCNGG 3 cut(s) 79, 194, 505
TaaI ACNGT 2 cut(s) 227, 459
TaiI ACGT 1 cut(s) 26
TaqI TCGA 2 cut(s) 65, 331
TasI AATT 2 cut(s) 252, 337
Tru1I TTAA 2 cut(s) 90, 559
Tru9I TTAA 2 cut(s) 90, 559
TscAI CASTG 1 cut(s) 230
TseFI GTSAC 2 cut(s) 146, 221
TseI GCWGC 2 cut(s) 288, 497
Tsp45I GTSAC 2 cut(s) 146, 221
TspDTI ATGAA 2 cut(s) 204, 417
TspRI CASTG 1 cut(s) 230
VneI GTGCAC 1 cut(s) 417
XcmI CCANNNNNNNNNTGG 1 cut(s) 513
XhoI CTCGAG 1 cut(s) 330
XspI CTAG 2 cut(s) 14, 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.