Rorug01G0453800

chloroplast organization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0007316
Physical Location & Seq
Forward (+)
54843486 .. 54845537
2052 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0453800.1

Sequence Viewer

Length: 801 bp
ATGGCGGAGCCAGAAGAGATACCAGGGGCGTCGCTCATGGAGAAGATCTACTCCCACCACCGCTCCTCGTCGTCTTCGTCGTCTTCCGACGACGACAAGCCGTCCAAGAAGGCCGATGACGTCAAGCACAAGGCTGAGGAACACGACATGCCGTCCGCTCACGATTCCGTCAAGTCCAACAAATTCAGGATCTTCGGCCGGGAAAAGCCCGTTCATAAGGTCCTCGGCGGTGGCAAACCTGCTGATGTCCTCCTATGGAGGGACAAGAAAGTATCTGGAAGTGTTCTTGGTGGAGCCACTGTGCTATGGATTTTCTTTGAGTTGCTTGAATACCATTTGATAACTCTGGTCTGCCATATGTTGATACTTTCTCTGGGCGTTTTCTTCCTGTGGTCCAATGCATCCTCCATTATCAACAAGGCTCCACCAGAAATCCCAAAAGTTCAAATTCCGGAGAAGTGTGTCCTTGATCTTGCCTCTGCACTGAGGATTGAACTCAACAAAGGGTTCTATGTTCTCCGGGAAATTGCATCTGGGAGAGATTTGAGGACATTCCTCATTGTTATAGTTGTCTTGTGGATTGTGTCAGTGATTGGCAAATGGTTCAACTTCCTGACCTTATTCTACTTATCCTTTGTCTTGCTGCACACGGTGCCAGTGATCTATGAGAAACATGATGTCCAGATTGATGCTTTTGCTGAGAAGGCATGGATTGAGATTAAGAAGCAGTATGCAGTGTTTGATGCGAATGTTTTGAGTAAGATACCCAAGGGTCCAGTGAAAGAGAGGAAGAAGGACTAG

Protein Analysis

266

Amino Acids

30.05

Weight (kDa)

8.85

Isoelectric Point (pI)

47.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 81 - 236 5.1e-57 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 123
Acc36I ACCTGC 1 cut(s) 247
AccB1I GGYRCC 1 cut(s) 652
AccBSI CCGCTC 2 cut(s) 63, 158
AccIII TCCGGA 1 cut(s) 451
AciI CCGC 4 cut(s) 5, 61, 156, 228
AclWI GGATC 1 cut(s) 197
AcoI YGGCCR 1 cut(s) 196
AcsI RAATTY 2 cut(s) 182, 447
AcyI GRCGYC 2 cut(s) 29, 120
AdeI CACNNNGTG 1 cut(s) 652
AfiI CCNNNNNNNGG 1 cut(s) 259
AgsI TTSAA 4 cut(s) 329, 446, 494, 607
AhdI GACNNNNNGTC 2 cut(s) 100, 151
AjnI CCWGG 1 cut(s) 22
AlwI GGATC 1 cut(s) 197
Aor13HI TCCGGA 1 cut(s) 451
AoxI GGCC 2 cut(s) 111, 196
ApeKI GCWGC 1 cut(s) 643
ApoI RAATTY 2 cut(s) 182, 447
AspS9I GGNCC 3 cut(s) 220, 393, 773
AsuC2I CCSGG 2 cut(s) 200, 521
AvaII GGWCC 3 cut(s) 220, 393, 773
BanI GGYRCC 1 cut(s) 652
BbsI GAAGAC 2 cut(s) 66, 75
BbvCI CCTCAGC 1 cut(s) 135
BbvI GCAGC 1 cut(s) 630
BceAI ACGGC 2 cut(s) 85, 136
BciT130I CCWGG 1 cut(s) 24
BcnI CCSGG 2 cut(s) 200, 521
BfaI CTAG 1 cut(s) 799
BfuAI ACCTGC 1 cut(s) 247
BglII AGATCT 1 cut(s) 45
BisI GCNGC 1 cut(s) 644
BlsI GCNGC 1 cut(s) 645
Bme1390I CCNGG 3 cut(s) 24, 200, 521
Bme18I GGWCC 3 cut(s) 220, 393, 773
BmeRI GACNNNNNGTC 2 cut(s) 100, 151
BmgT120I GGNCC 3 cut(s) 220, 393, 773
BmiI GGNNCC 5 cut(s) 9, 295, 423, 654, 774
BmrFI CCNGG 3 cut(s) 24, 200, 521
BmsI GCATC 4 cut(s) 410, 539, 679, 733
BpiI GAAGAC 2 cut(s) 66, 75
Bpu10I CCTNAGC 1 cut(s) 135
BpuMI CCSGG 2 cut(s) 200, 521
BsaHI GRCGYC 2 cut(s) 29, 120
BsaJI CCNNGG 3 cut(s) 23, 223, 768
BsaWI WCCGGW 1 cut(s) 451
BsaXI ACNNNNNCTCC 4 cut(s) 47, 77, 285, 315
Bsc4I CCNNNNNNNGG 1 cut(s) 259
Bse1I ACTGG 2 cut(s) 656, 776
BseAI TCCGGA 1 cut(s) 451
BseBI CCWGG 1 cut(s) 24
BseDI CCNNGG 3 cut(s) 23, 223, 768
BseGI GGATG 1 cut(s) 401
BseLI CCNNNNNNNGG 1 cut(s) 259
BseMII CTCAG 3 cut(s) 126, 476, 690
BseNI ACTGG 2 cut(s) 656, 776
BseRI GAGGAG 1 cut(s) 55
BseX3I CGGCCG 1 cut(s) 196
BseXI GCAGC 1 cut(s) 630
BsgI GTGCAG 2 cut(s) 465, 629
Bsh1285I CGRYCG 1 cut(s) 199
BshFI GGCC 2 cut(s) 113, 198
BshNI GGYRCC 1 cut(s) 652
BsiEI CGRYCG 1 cut(s) 199
BsiSI CCGG 3 cut(s) 199, 452, 520
BslFI GGGAC 1 cut(s) 275
BslI CCNNNNNNNGG 1 cut(s) 259
BsmFI GGGAC 1 cut(s) 275
BsnI GGCC 2 cut(s) 113, 198
Bsp13I TCCGGA 1 cut(s) 451
Bsp143I GATC 4 cut(s) 45, 189, 469, 660
BspACI CCGC 4 cut(s) 5, 61, 156, 228
BspANI GGCC 2 cut(s) 113, 198
BspCNI CTCAG 3 cut(s) 127, 477, 691
BspEI TCCGGA 1 cut(s) 451
BspLI GGNNCC 5 cut(s) 9, 295, 423, 654, 774
BspMI ACCTGC 1 cut(s) 247
BspPI GGATC 1 cut(s) 197
BspT107I GGYRCC 1 cut(s) 652
BsrBI CCGCTC 2 cut(s) 63, 158
BsrI ACTGG 2 cut(s) 656, 776
BssECI CCNNGG 3 cut(s) 23, 223, 768
BssMI GATC 4 cut(s) 45, 189, 469, 660
BssNI GRCGYC 2 cut(s) 29, 120
BssT1I CCWWGG 1 cut(s) 768
Bst2UI CCWGG 1 cut(s) 24
Bst4CI ACNGT 2 cut(s) 301, 652
Bst6I CTCTTC 1 cut(s) 9
BstACI GRCGYC 2 cut(s) 29, 120
BstAPI GCANNNNNTGC 1 cut(s) 652
BstDEI CTNAG 3 cut(s) 135, 485, 699
BstF5I GGATG 1 cut(s) 401
BstKTI GATC 4 cut(s) 48, 192, 472, 663
BstMBI GATC 4 cut(s) 45, 189, 469, 660
BstMCI CGRYCG 1 cut(s) 199
BstMWI GCNNNNNNNGC 2 cut(s) 652, 704
BstNI CCWGG 1 cut(s) 24
BstNSI RCATGY 1 cut(s) 151
BstSCI CCNGG 3 cut(s) 22, 198, 519
BstV1I GCAGC 1 cut(s) 630
BstV2I GAAGAC 2 cut(s) 66, 75
BstX2I RGATCY 2 cut(s) 45, 189
BstYI RGATCY 2 cut(s) 45, 189
BstZI CGGCCG 1 cut(s) 196
BsuRI GGCC 2 cut(s) 113, 198
BtsCI GGATG 1 cut(s) 401
BtsI GCAGTG 1 cut(s) 741
BtsIMutI CAGTG 6 cut(s) 297, 482, 594, 663, 741, 783
BveI ACCTGC 1 cut(s) 247
Cfr13I GGNCC 3 cut(s) 220, 393, 773
CseI GACGC 1 cut(s) 18
CviAII CATG 4 cut(s) 37, 148, 674, 708
CviJI RGCY 8 cut(s) 10, 100, 113, 134, 198, 208, 296, 422
CviKI_1 RGCY 8 cut(s) 10, 100, 113, 134, 198, 208, 296, 422
DdeI CTNAG 3 cut(s) 135, 485, 699
DpnI GATC 4 cut(s) 47, 191, 471, 662
DpnII GATC 4 cut(s) 45, 189, 469, 660
DraIII CACNNNGTG 1 cut(s) 652
DriI GACNNNNNGTC 2 cut(s) 100, 151
EaeI YGGCCR 1 cut(s) 196
EagI CGGCCG 1 cut(s) 196
Eam1104I CTCTTC 1 cut(s) 9
Eam1105I GACNNNNNGTC 2 cut(s) 100, 151
EarI CTCTTC 1 cut(s) 9
EciI GGCGGA 1 cut(s) 20
EclXI CGGCCG 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 768
Eco47I GGWCC 3 cut(s) 220, 393, 773
Eco52I CGGCCG 1 cut(s) 196
EcoO109I RGGNCCY 1 cut(s) 220
EcoRII CCWGG 1 cut(s) 22
EcoT14I CCWWGG 1 cut(s) 768
EcoT22I ATGCAT 1 cut(s) 403
ErhI CCWWGG 1 cut(s) 768
FaeI CATG 4 cut(s) 40, 151, 677, 711
FaqI GGGAC 1 cut(s) 275
FatI CATG 4 cut(s) 36, 147, 673, 707
FauNDI CATATG 1 cut(s) 357
Fnu4HI GCNGC 1 cut(s) 644
FokI GGATG 1 cut(s) 388
Fsp4HI GCNGC 1 cut(s) 644
FspBI CTAG 1 cut(s) 799
GluI GCNGC 1 cut(s) 644
HaeIII GGCC 2 cut(s) 113, 198
HapII CCGG 3 cut(s) 199, 452, 520
HgaI GACGC 1 cut(s) 18
Hin1I GRCGYC 2 cut(s) 29, 120
Hin1II CATG 4 cut(s) 40, 151, 677, 711
HinfI GANTC 1 cut(s) 164
HpaII CCGG 3 cut(s) 199, 452, 520
Hpy188I TCNGA 1 cut(s) 88
Hpy188III TCNNGA 6 cut(s) 161, 187, 276, 452, 613, 682
Hpy99I CGWCG 5 cut(s) 34, 73, 82, 92, 95
HpyAV CCTTC 3 cut(s) 103, 697, 787
HpyCH4III ACNGT 2 cut(s) 301, 652
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 5 cut(s) 401, 482, 530, 646, 734
HpyF10VI GCNNNNNNNGC 2 cut(s) 652, 704
HpyF3I CTNAG 3 cut(s) 135, 485, 699
HpySE526I ACGT 1 cut(s) 120
Hsp92I GRCGYC 2 cut(s) 29, 120
Hsp92II CATG 4 cut(s) 40, 151, 677, 711
Kpn2I TCCGGA 1 cut(s) 451
Kzo9I GATC 4 cut(s) 45, 189, 469, 660
LmnI GCTCC 4 cut(s) 7, 68, 293, 427
Lsp1109I GCAGC 1 cut(s) 630
LweI GCATC 4 cut(s) 410, 539, 679, 733
MaeI CTAG 1 cut(s) 799
MaeII ACGT 1 cut(s) 120
MalI GATC 4 cut(s) 47, 191, 471, 662
MbiI CCGCTC 2 cut(s) 63, 158
MboI GATC 4 cut(s) 45, 189, 469, 660
MboII GAAGA 6 cut(s) 26, 55, 66, 75, 184, 376
MflI RGATCY 2 cut(s) 45, 189
MluCI AATT 3 cut(s) 182, 447, 525
MmeI TCCRAC 2 cut(s) 111, 201
Mph1103I ATGCAT 1 cut(s) 403
MroI TCCGGA 1 cut(s) 451
MseI TTAA 1 cut(s) 720
MspI CCGG 3 cut(s) 199, 452, 520
MspR9I CCNGG 3 cut(s) 24, 200, 521
MvaI CCWGG 1 cut(s) 24
MwoI GCNNNNNNNGC 2 cut(s) 652, 704
NciI CCSGG 2 cut(s) 200, 521
NdeI CATATG 1 cut(s) 357
NdeII GATC 4 cut(s) 45, 189, 469, 660
NlaIII CATG 4 cut(s) 40, 151, 677, 711
NlaIV GGNNCC 5 cut(s) 9, 295, 423, 654, 774
NmeAIII GCCGAG 1 cut(s) 204
NsiI ATGCAT 1 cut(s) 403
NspI RCATGY 1 cut(s) 151
PcsI WCGNNNNNNNCGW 2 cut(s) 74, 77
PfeI GAWTC 1 cut(s) 164
PfoI TCCNGGA 1 cut(s) 519
PkrI GCNGC 1 cut(s) 645
PpuMI RGGWCCY 1 cut(s) 220
Psp5II RGGWCCY 1 cut(s) 220
Psp6I CCWGG 1 cut(s) 22
PspGI CCWGG 1 cut(s) 22
PspN4I GGNNCC 5 cut(s) 9, 295, 423, 654, 774
PspPI GGNCC 3 cut(s) 220, 393, 773
PspPPI RGGWCCY 1 cut(s) 220
PsuI RGATCY 2 cut(s) 45, 189
SaqAI TTAA 1 cut(s) 720
SatI GCNGC 1 cut(s) 644
Sau3AI GATC 4 cut(s) 45, 189, 469, 660
Sau96I GGNCC 3 cut(s) 220, 393, 773
ScrFI CCNGG 3 cut(s) 24, 200, 521
SetI ASST 4 cut(s) 123, 222, 241, 620
SfaNI GCATC 4 cut(s) 410, 539, 679, 733
SinI GGWCC 3 cut(s) 220, 393, 773
Sse9I AATT 3 cut(s) 182, 447, 525
SsiI CCGC 4 cut(s) 5, 61, 156, 228
SspMI CTAG 1 cut(s) 799
StyD4I CCNGG 3 cut(s) 22, 198, 519
StyI CCWWGG 1 cut(s) 768
TaaI ACNGT 2 cut(s) 301, 652
TaiI ACGT 1 cut(s) 123
TasI AATT 3 cut(s) 182, 447, 525
TfiI GAWTC 1 cut(s) 164
Tru1I TTAA 1 cut(s) 720
Tru9I TTAA 1 cut(s) 720
TscAI CASTG 6 cut(s) 304, 489, 594, 663, 741, 783
TseI GCWGC 1 cut(s) 643
TspDTI ATGAA 1 cut(s) 203
TspGWI ACGGA 1 cut(s) 157
TspRI CASTG 6 cut(s) 304, 489, 594, 663, 741, 783
VpaK11BI GGWCC 3 cut(s) 220, 393, 773
XapI RAATTY 2 cut(s) 182, 447
XceI RCATGY 1 cut(s) 151
XspI CTAG 1 cut(s) 799
ZraI GACGTC 1 cut(s) 121
Zsp2I ATGCAT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.