Rorug02G0305000

GTP1/OBG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007549
Physical Location & Seq
Forward (+)
34408124 .. 34410124
2001 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0305000.1

Sequence Viewer

Length: 294 bp
ATGTTCCTCTTTCTCCGATCAGCTTCCTGGAGCGCTCAGCCAGTCTACAGAGACAGAACCTCTATTATGTATGGAGACATCGTCTACACTTGGAGACAGACACTTGAACGATGCACCAGACTTGCTTCTGCTCTTGCCCAACTTGGAATTTCTCGAGGCGATGTGAATGCATTTGAGCTCGCCTTCTTCGTTTGGGTTCCAATTGGCCAGGGTCATTTTAGGAATTTCACTTCCATATGGGGCCCACCTGTGCTGAGTTGGATCGCCACGTCAGCAGCTAACGGCAGAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GTP1_OBG MMR_HSR1

Protein Analysis

97

Amino Acids

11.02

Weight (kDa)

10.24

Isoelectric Point (pI)

22.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 15 - 55 4.3e-06 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 45, 84
AclWI GGATC 1 cut(s) 269
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 2 cut(s) 147, 223
AfeI AGCGCT 1 cut(s) 34
AgsI TTSAA 1 cut(s) 107
AjiI CACGTC 1 cut(s) 270
AjnI CCWGG 2 cut(s) 26, 207
AluBI AGCT 3 cut(s) 23, 178, 278
AluI AGCT 3 cut(s) 23, 178, 278
Alw21I GWGCWC 1 cut(s) 180
Alw26I GTCTC 3 cut(s) 45, 69, 88
AlwI GGATC 1 cut(s) 269
Ama87I CYCGRG 1 cut(s) 153
Aor51HI AGCGCT 1 cut(s) 34
AoxI GGCC 2 cut(s) 205, 241
ApaI GGGCCC 1 cut(s) 245
ApeKI GCWGC 1 cut(s) 275
ApoI RAATTY 2 cut(s) 147, 223
AspLEI GCGC 1 cut(s) 35
AspS9I GGNCC 2 cut(s) 241, 242
AvaI CYCGRG 1 cut(s) 153
BaeGI GKGCMC 1 cut(s) 245
BalI TGGCCA 1 cut(s) 207
BanII GRGCYC 2 cut(s) 180, 245
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 1 cut(s) 287
BcgI CGANNNNNNTGC 2 cut(s) 149, 183
BciT130I CCWGG 2 cut(s) 28, 209
BcoDI GTCTC 3 cut(s) 45, 69, 88
BfmI CTRYAG 1 cut(s) 46
BfoI RGCGCY 1 cut(s) 36
BisI GCNGC 1 cut(s) 276
BlpI GCTNAGC 1 cut(s) 36
BlsI GCNGC 1 cut(s) 277
Bme1390I CCNGG 2 cut(s) 28, 209
BmeT110I CYCGRG 1 cut(s) 153
BmgBI CACGTC 1 cut(s) 270
BmgT120I GGNCC 2 cut(s) 241, 242
BmiI GGNNCC 3 cut(s) 198, 242, 243
BmrFI CCNGG 2 cut(s) 28, 209
BmsI GCATC 1 cut(s) 101
BpmI CTGGAG 1 cut(s) 49
Bpu1102I GCTNAGC 1 cut(s) 36
BsaJI CCNNGG 1 cut(s) 208
BsaXI ACNNNNNCTCC 2 cut(s) 66, 96
Bse1I ACTGG 1 cut(s) 41
BseBI CCWGG 2 cut(s) 28, 209
BseDI CCNNGG 1 cut(s) 208
BseMII CTCAG 2 cut(s) 50, 245
BseNI ACTGG 1 cut(s) 41
BseSI GKGCMC 1 cut(s) 245
BseXI GCAGC 1 cut(s) 287
BshFI GGCC 2 cut(s) 207, 243
BsiHKAI GWGCWC 1 cut(s) 180
BsiHKCI CYCGRG 1 cut(s) 153
BsmAI GTCTC 3 cut(s) 45, 69, 88
BsmI GAATGC 1 cut(s) 172
BsnI GGCC 2 cut(s) 207, 243
BsoBI CYCGRG 1 cut(s) 153
Bsp120I GGGCCC 1 cut(s) 241
Bsp1286I GDGCHC 2 cut(s) 180, 245
Bsp143I GATC 2 cut(s) 17, 261
Bsp1720I GCTNAGC 1 cut(s) 36
BspANI GGCC 2 cut(s) 207, 243
BspCNI CTCAG 2 cut(s) 49, 246
BspLI GGNNCC 3 cut(s) 198, 242, 243
BspPI GGATC 1 cut(s) 269
BsrI ACTGG 1 cut(s) 41
BssECI CCNNGG 1 cut(s) 208
BssMI GATC 2 cut(s) 17, 261
Bst2UI CCWGG 2 cut(s) 28, 209
BstC8I GCNNGC 1 cut(s) 180
BstDEI CTNAG 2 cut(s) 36, 254
BstH2I RGCGCY 1 cut(s) 36
BstHHI GCGC 1 cut(s) 35
BstKTI GATC 2 cut(s) 20, 264
BstMAI GTCTC 3 cut(s) 45, 69, 88
BstMBI GATC 2 cut(s) 17, 261
BstMWI GCNNNNNNNGC 1 cut(s) 272
BstNI CCWGG 2 cut(s) 28, 209
BstSCI CCNGG 2 cut(s) 26, 207
BstSFI CTRYAG 1 cut(s) 46
BstSLI GKGCMC 1 cut(s) 245
BstV1I GCAGC 1 cut(s) 287
BsuRI GGCC 2 cut(s) 207, 243
BtgZI GCGATG 1 cut(s) 174
BtrI CACGTC 1 cut(s) 270
Cac8I GCNNGC 1 cut(s) 180
CfoI GCGC 1 cut(s) 35
Cfr13I GGNCC 2 cut(s) 241, 242
CviJI RGCY 6 cut(s) 23, 40, 178, 207, 243, 278
CviKI_1 RGCY 6 cut(s) 23, 40, 178, 207, 243, 278
DdeI CTNAG 2 cut(s) 36, 254
DpnI GATC 2 cut(s) 19, 263
DpnII GATC 2 cut(s) 17, 261
EaeI YGGCCR 1 cut(s) 205
Ecl136II GAGCTC 1 cut(s) 178
Eco24I GRGCYC 2 cut(s) 180, 245
Eco47III AGCGCT 1 cut(s) 34
Eco53kI GAGCTC 1 cut(s) 178
Eco88I CYCGRG 1 cut(s) 153
EcoICRI GAGCTC 1 cut(s) 178
EcoO109I RGGNCCY 1 cut(s) 241
EcoRII CCWGG 2 cut(s) 26, 207
EcoT22I ATGCAT 1 cut(s) 172
EcoT38I GRGCYC 2 cut(s) 180, 245
FaiI YATR 4 cut(s) 68, 72, 236, 238
FauNDI CATATG 1 cut(s) 236
FblI GTMKAC 2 cut(s) 45, 84
Fnu4HI GCNGC 1 cut(s) 276
FriOI GRGCYC 2 cut(s) 180, 245
Fsp4HI GCNGC 1 cut(s) 276
GlaI GCGC 1 cut(s) 34
GluI GCNGC 1 cut(s) 276
GsuI CTGGAG 1 cut(s) 49
HaeII RGCGCY 1 cut(s) 36
HaeIII GGCC 2 cut(s) 207, 243
HhaI GCGC 1 cut(s) 35
Hin6I GCGC 1 cut(s) 33
HinP1I GCGC 1 cut(s) 33
Hpy166II GTNNAC 2 cut(s) 46, 85
Hpy188I TCNGA 1 cut(s) 17
Hpy188III TCNNGA 1 cut(s) 153
Hpy8I GTNNAC 2 cut(s) 46, 85
HpyAV CCTTC 1 cut(s) 193
HpyCH4IV ACGT 1 cut(s) 269
HpyCH4V TGCA 2 cut(s) 114, 170
HpyF10VI GCNNNNNNNGC 1 cut(s) 272
HpyF3I CTNAG 2 cut(s) 36, 254
HpySE526I ACGT 1 cut(s) 269
HspAI GCGC 1 cut(s) 33
Kzo9I GATC 2 cut(s) 17, 261
LmnI GCTCC 1 cut(s) 30
LpnPI CCDG 7 cut(s) 13, 40, 54, 130, 194, 221, 261
Lsp1109I GCAGC 1 cut(s) 287
LweI GCATC 1 cut(s) 101
MaeII ACGT 1 cut(s) 269
MalI GATC 2 cut(s) 19, 263
MboI GATC 2 cut(s) 17, 261
MboII GAAGA 1 cut(s) 178
MfeI CAATTG 1 cut(s) 201
MhlI GDGCHC 2 cut(s) 180, 245
MlsI TGGCCA 1 cut(s) 207
MluCI AATT 4 cut(s) 147, 201, 223, 289
MluNI TGGCCA 1 cut(s) 207
MmeI TCCRAC 1 cut(s) 239
MnlI CCTC 3 cut(s) 17, 70, 149
Mox20I TGGCCA 1 cut(s) 207
Mph1103I ATGCAT 1 cut(s) 172
MscI TGGCCA 1 cut(s) 207
MseI TTAA 1 cut(s) 292
Msp20I TGGCCA 1 cut(s) 207
MspR9I CCNGG 2 cut(s) 28, 209
MunI CAATTG 1 cut(s) 201
Mva1269I GAATGC 1 cut(s) 172
MvaI CCWGG 2 cut(s) 28, 209
MwoI GCNNNNNNNGC 1 cut(s) 272
NdeI CATATG 1 cut(s) 236
NdeII GATC 2 cut(s) 17, 261
NlaIV GGNNCC 3 cut(s) 198, 242, 243
NsiI ATGCAT 1 cut(s) 172
PaeR7I CTCGAG 1 cut(s) 153
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PctI GAATGC 1 cut(s) 172
PflFI GACNNNGTC 1 cut(s) 80
PfoI TCCNGGA 1 cut(s) 26
PkrI GCNGC 1 cut(s) 277
Psp124BI GAGCTC 1 cut(s) 180
Psp6I CCWGG 2 cut(s) 26, 207
PspGI CCWGG 2 cut(s) 26, 207
PspN4I GGNNCC 3 cut(s) 198, 242, 243
PspOMI GGGCCC 1 cut(s) 241
PspPI GGNCC 2 cut(s) 241, 242
PsyI GACNNNGTC 1 cut(s) 80
SacI GAGCTC 1 cut(s) 180
SaqAI TTAA 1 cut(s) 292
SatI GCNGC 1 cut(s) 276
Sau3AI GATC 2 cut(s) 17, 261
Sau96I GGNCC 2 cut(s) 241, 242
ScrFI CCNGG 2 cut(s) 28, 209
SduI GDGCHC 2 cut(s) 180, 245
SetI ASST 6 cut(s) 25, 62, 180, 250, 272, 280
SfaNI GCATC 1 cut(s) 101
SfcI CTRYAG 1 cut(s) 46
Sfr274I CTCGAG 1 cut(s) 153
SlaI CTCGAG 1 cut(s) 153
SmlI CTYRAG 1 cut(s) 153
SmoI CTYRAG 1 cut(s) 153
Sse9I AATT 4 cut(s) 147, 201, 223, 289
SstI GAGCTC 1 cut(s) 180
StyD4I CCNGG 2 cut(s) 26, 207
TaiI ACGT 1 cut(s) 272
TaqI TCGA 1 cut(s) 154
TasI AATT 4 cut(s) 147, 201, 223, 289
Tru1I TTAA 1 cut(s) 292
Tru9I TTAA 1 cut(s) 292
TseI GCWGC 1 cut(s) 275
Tth111I GACNNNGTC 1 cut(s) 80
XapI RAATTY 2 cut(s) 147, 223
XhoI CTCGAG 1 cut(s) 153
XmiI GTMKAC 2 cut(s) 45, 84
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.