Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0013728

Physical Location & Seq

Genomic Coordinates Reverse (-)

3455873 .. 3458208

Gene Structure 2336 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0024800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1020 bp

ATGGCTATTTCAATGCTCTATGGGGCTCAAAATATGAAACTCACTTCTGGCATAGCACCAACTGGGTTAGGTTCCGGGGGAAATTTTCAATATGGGTCTTTCCCCAAAAAGGTTTCAGTTAGTTATGGAAGGAATTCCAAGGCTAGAACTTTGGGACCCATATGCAGCGTCTCATCCTCAAGGCCTGCTTCTCAGCCGAGGTTTATACAGCACAAGCAAGAAGCATTTTGGTTCTATAGGTTTTTATCTATAGTCTATGATCATGTGATAAACCCTGGGCATTGGACTGAGGATATGAGAGACGATGCTCTCGAGCCGGCTGATCTTTATGACAGGAATATGACAGTGGTAGATGTTGGAGGTGGAACTGGGTTCACAACACTGGGGATTGTGAAGCATGTGGATGCCAAAAATGTTACAATTCTTGACCAATCACCGCACCAGCTTGCCAAGGCTAAGGAGAAGGAGCCCTTGAAAGAGTGCAAGATTATCGAAGGTGATGCCGAGGATCTGCCATTCCCAACTGATTATGCGGATAGATATGTCTCTGCTGGAAGTATAGAGTACTGGCCGGATCCACAGCGTGGAATCAAGGAAGCATACAGGGTACTAAAAATAGGAGGAAAAGCCTGTCTTATAGGCCCTGTGTACCCAACCTTCTGGCTGTCTCGTTTTTTCGCAGACGTGTGGATGCTCTTCCCAACAGAGGAAGAATACATTGAGTGGTTCCAAAAGGCAGGTTTCAAAGATGTTAAACTAAAAAGAATTGGTCCAAAGTGGTACCGTGGAGTTCGCCGGCATGGCCTGATTATGGGTTGCTCTGTGACAGGTGTGAAGCCCTTAAGTGGGGATTCCCCTCTCCAGCTTGGTCCAAAGGTTGAGGATGTGAGGAAGCCCGTCAATCCATTTGTGTTTCTTTTCCGGCTAATTCTTGGAGCAACTGCAGGAGCTTACTATGTGCTGGTTCCCATTTACATGTGGATCAAAGATCAAATTGTTCCAAAGGGCAAACCTATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001101 GO:0002230 GO:0002238 GO:0002239 GO:0002252 GO:0002376 GO:0002682 GO:0002697 GO:0002831 GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008219 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009615 GO:0009620 GO:0009626 GO:0009628 GO:0009637 GO:0009642 GO:0009646 GO:0009719 GO:0009725 GO:0009751 GO:0009987 GO:0010033 GO:0012501 GO:0014070 GO:0016020 GO:0023052 GO:0030054 GO:0031347 GO:0032101 GO:0032870 GO:0033554 GO:0034050 GO:0035690 GO:0036094 GO:0042221 GO:0042493 GO:0043207 GO:0043900 GO:0044464 GO:0045087 GO:0046677 GO:0048583 GO:0050688 GO:0050691 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071407 GO:0071446 GO:0071495 GO:0071944 GO:0080134 GO:0097159 GO:0098542 GO:1901265 GO:1901363 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NB-ARC

Protein Analysis

339

Amino Acids

38.02

Weight (kDa)

9.18

Isoelectric Point (pI)

37.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_23	PF13489	110 - 252	6.1e-06	Methyltransferase domain
Methyltransf_31	PF13847	111 - 215	1.5e-12	Methyltransferase domain
Ubie_methyltran	PF01209	112 - 213	2.5e-12	ubiE/COQ5 methyltransferase family
Methyltransf_25	PF13649	116 - 208	3.8e-17	Methyltransferase domain
Methyltransf_11	PF08241	117 - 211	1.4e-19	Methyltransferase domain
Methyltransf_12	PF08242	117 - 209	2.2e-10	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013728)

Species	Orthologous Gene IDs
malus_domestica	MD13G1128100.v1.1
rosa_chinensis	RchiOBHm_Chr4g0400321
rosa_laevigata	RLG00000019087 RLG00000019851 RLG00000029817 RLG00000036223
rosa_multiflora	Rmu_co8364043.1_g000001 Rmu_sc0000446.1_g000020 Rmu_sc0006567.1_g000017 Rmu_ssc0000245.1_g000016 Rmu_ssc0000245.1_g000028
rosa_roxburghii	Rroxscaffold_3G00246800 Rroxscaffold_3G00250210
rosa_rugosa	Rorug04G0024800
rosa_samantha	Rh2DG376000 Rh5DG042900 Rh6BG179100 Rh6DG076300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	728
Acc65I	GGTACC	1 cut(s)	780
AccB1I	GGYRCC	1 cut(s)	780
AccB7I	CCANNNNNTGG	1 cut(s)	584
AciI	CCGC	2 cut(s)	437, 533
AclWI	GGATC	4 cut(s)	516, 569, 582, 989
AcoI	YGGCCR	1 cut(s)	569
AcsI	RAATTY	2 cut(s)	82, 133
AdeI	CACNNNGTG	1 cut(s)	584
AfaI	GTAC	4 cut(s)	566, 609, 650, 782
AfiI	CCNNNNNNNGG	6 cut(s)	109, 584, 706, 811, 845, 846
AflII	CTTAAG	1 cut(s)	841
AflIII	ACRYGT	2 cut(s)	684, 975
AgsI	TTSAA	4 cut(s)	12, 89, 475, 745
AjiI	CACGTC	1 cut(s)	685
AjnI	CCWGG	1 cut(s)	274
AluBI	AGCT	3 cut(s)	445, 865, 950
AluI	AGCT	3 cut(s)	445, 865, 950
Alw26I	GTCTC	4 cut(s)	175, 294, 550, 672
AlwI	GGATC	4 cut(s)	516, 569, 582, 989
Ama87I	CYCGRG	1 cut(s)	311
AoxI	GGCC	4 cut(s)	182, 569, 640, 802
ApeKI	GCWGC	1 cut(s)	165
ApoI	RAATTY	2 cut(s)	82, 133
Asp700I	GAANNNNTTC	1 cut(s)	133
Asp718I	GGTACC	1 cut(s)	780
AspS9I	GGNCC	4 cut(s)	155, 641, 770, 869
AsuC2I	CCSGG	1 cut(s)	76
AsuHPI	GGTGA	2 cut(s)	426, 509
AvaI	CYCGRG	1 cut(s)	311
AvaII	GGWCC	3 cut(s)	155, 770, 869
BamHI	GGATCC	1 cut(s)	574
BanI	GGYRCC	1 cut(s)	780
BanII	GRGCYC	2 cut(s)	28, 471
BarI	GAAGNNNNNNTAC	2 cut(s)	641, 673
BbvI	GCAGC	1 cut(s)	177
BcgI	CGANNNNNNTGC	4 cut(s)	472, 482, 506, 516
BciT130I	CCWGG	1 cut(s)	276
BclI	TGATCA	1 cut(s)	259
BcnI	CCSGG	1 cut(s)	76
BcoDI	GTCTC	4 cut(s)	175, 294, 550, 672
BfaI	CTAG	1 cut(s)	144
BfmI	CTRYAG	3 cut(s)	235, 249, 942
BfrI	CTTAAG	1 cut(s)	841
BfuAI	ACCTGC	1 cut(s)	728
BglI	GCCNNNNNGGC	1 cut(s)	801
BisI	GCNGC	1 cut(s)	166
BlsI	GCNGC	1 cut(s)	167
BmcAI	AGTACT	1 cut(s)	566
Bme1390I	CCNGG	2 cut(s)	76, 276
Bme18I	GGWCC	3 cut(s)	155, 770, 869
BmeT110I	CYCGRG	1 cut(s)	311
BmgBI	CACGTC	1 cut(s)	685
BmgT120I	GGNCC	4 cut(s)	155, 641, 770, 869
BmiI	GGNNCC	8 cut(s)	73, 156, 157, 468, 576, 728, 782, 966
BmrFI	CCNGG	2 cut(s)	76, 276
BmrI	ACTGGG	3 cut(s)	72, 378, 392
BmsI	GCATC	4 cut(s)	295, 394, 490, 681
BmuI	ACTGGG	3 cut(s)	72, 378, 392
BpmI	CTGGAG	1 cut(s)	845
Bpu10I	CCTNAGC	1 cut(s)	456
BpuEI	CTTGAG	1 cut(s)	163
BpuMI	CCSGG	1 cut(s)	76
BsaJI	CCNNGG	8 cut(s)	75, 138, 197, 274, 275, 450, 504, 784
Bsc4I	CCNNNNNNNGG	6 cut(s)	109, 584, 706, 811, 845, 846
Bse118I	RCCGGY	2 cut(s)	316, 795
Bse1I	ACTGG	4 cut(s)	67, 373, 387, 572
BseBI	CCWGG	1 cut(s)	276
BseDI	CCNNGG	8 cut(s)	75, 138, 197, 274, 275, 450, 504, 784
BseGI	GGATG	4 cut(s)	173, 409, 696, 889
BseLI	CCNNNNNNNGG	6 cut(s)	109, 584, 706, 811, 845, 846
BseMII	CTCAG	2 cut(s)	206, 279
BseNI	ACTGG	4 cut(s)	67, 373, 387, 572
BseXI	GCAGC	1 cut(s)	177
BshFI	GGCC	4 cut(s)	184, 571, 642, 804
BshNI	GGYRCC	1 cut(s)	780
BsiHKCI	CYCGRG	1 cut(s)	311
BsiSI	CCGG	5 cut(s)	75, 317, 572, 796, 922
BslFI	GGGAC	1 cut(s)	168
BslI	CCNNNNNNNGG	6 cut(s)	109, 584, 706, 811, 845, 846
BsmAI	GTCTC	4 cut(s)	175, 294, 550, 672
BsmBI	CGTCTC	2 cut(s)	175, 294
BsmFI	GGGAC	1 cut(s)	168
BsnI	GGCC	4 cut(s)	184, 571, 642, 804
BsoBI	CYCGRG	1 cut(s)	311
Bsp1286I	GDGCHC	2 cut(s)	28, 471
Bsp143I	GATC	6 cut(s)	259, 322, 508, 574, 981, 988
BspACI	CCGC	2 cut(s)	437, 533
BspANI	GGCC	4 cut(s)	184, 571, 642, 804
BspCNI	CTCAG	2 cut(s)	205, 280
BspLI	GGNNCC	8 cut(s)	73, 156, 157, 468, 576, 728, 782, 966
BspMAI	CTGCAG	1 cut(s)	946
BspMI	ACCTGC	1 cut(s)	728
BspPI	GGATC	4 cut(s)	516, 569, 582, 989
BspQI	GCTCTTC	1 cut(s)	701
BspT107I	GGYRCC	1 cut(s)	780
BspTI	CTTAAG	1 cut(s)	841
BsrFI	RCCGGY	2 cut(s)	316, 795
BsrI	ACTGG	4 cut(s)	67, 373, 387, 572
BssAI	RCCGGY	2 cut(s)	316, 795
BssECI	CCNNGG	8 cut(s)	75, 138, 197, 274, 275, 450, 504, 784
BssMI	GATC	6 cut(s)	259, 322, 508, 574, 981, 988
BssT1I	CCWWGG	2 cut(s)	138, 450
Bst2UI	CCWGG	1 cut(s)	276
Bst4CI	ACNGT	2 cut(s)	346, 785
Bst6I	CTCTTC	1 cut(s)	701
BstAFI	CTTAAG	1 cut(s)	841
BstC8I	GCNNGC	4 cut(s)	186, 318, 447, 797
BstDEI	CTNAG	3 cut(s)	192, 288, 456
BstDSI	CCRYGG	1 cut(s)	784
BstF5I	GGATG	4 cut(s)	173, 409, 696, 889
BstKTI	GATC	6 cut(s)	262, 325, 511, 577, 984, 991
BstMAI	GTCTC	4 cut(s)	175, 294, 550, 672
BstMBI	GATC	6 cut(s)	259, 322, 508, 574, 981, 988
BstMWI	GCNNNNNNNGC	1 cut(s)	801
BstNI	CCWGG	1 cut(s)	276
BstNSI	RCATGY	2 cut(s)	401, 979
BstSCI	CCNGG	2 cut(s)	74, 274
BstSFI	CTRYAG	3 cut(s)	235, 249, 942
BstV1I	GCAGC	1 cut(s)	177
BstX2I	RGATCY	2 cut(s)	508, 574
BstXI	CCANNNNNNTGG	1 cut(s)	660
BstYI	RGATCY	2 cut(s)	508, 574
BsuRI	GGCC	4 cut(s)	184, 571, 642, 804
BtgI	CCRYGG	1 cut(s)	784
BtrI	CACGTC	1 cut(s)	685
BtsCI	GGATG	4 cut(s)	173, 409, 696, 889
BtsIMutI	CAGTG	2 cut(s)	351, 380
BveI	ACCTGC	1 cut(s)	728
Cac8I	GCNNGC	4 cut(s)	186, 318, 447, 797
Cfr10I	RCCGGY	2 cut(s)	316, 795
Cfr13I	GGNCC	4 cut(s)	155, 641, 770, 869
CseI	GACGC	1 cut(s)	157
Csp6I	GTAC	4 cut(s)	565, 608, 649, 781
CviAII	CATG	4 cut(s)	263, 398, 800, 976
CviQI	GTAC	4 cut(s)	565, 608, 649, 781
DdeI	CTNAG	3 cut(s)	192, 288, 456
DpnI	GATC	6 cut(s)	261, 324, 510, 576, 983, 990
DpnII	GATC	6 cut(s)	259, 322, 508, 574, 981, 988
DraIII	CACNNNGTG	1 cut(s)	584
EaeI	YGGCCR	1 cut(s)	569
Eam1104I	CTCTTC	1 cut(s)	701
EarI	CTCTTC	1 cut(s)	701
Eco130I	CCWWGG	2 cut(s)	138, 450
Eco147I	AGGCCT	1 cut(s)	184
Eco24I	GRGCYC	2 cut(s)	28, 471
Eco47I	GGWCC	3 cut(s)	155, 770, 869
Eco88I	CYCGRG	1 cut(s)	311
EcoO109I	RGGNCCY	2 cut(s)	155, 641
EcoRI	GAATTC	1 cut(s)	133
EcoRII	CCWGG	1 cut(s)	274
EcoT14I	CCWWGG	2 cut(s)	138, 450
EcoT38I	GRGCYC	2 cut(s)	28, 471
ErhI	CCWWGG	2 cut(s)	138, 450
Esp3I	CGTCTC	2 cut(s)	175, 294
FaeI	CATG	4 cut(s)	266, 401, 803, 979
FalI	AAGNNNNNCTT	6 cut(s)	172, 204, 455, 487, 618, 650
FaqI	GGGAC	1 cut(s)	168
FatI	CATG	4 cut(s)	262, 397, 799, 975
FauNDI	CATATG	1 cut(s)	161
FbaI	TGATCA	1 cut(s)	259
Fnu4HI	GCNGC	1 cut(s)	166
FokI	GGATG	4 cut(s)	160, 416, 703, 896
FriOI	GRGCYC	2 cut(s)	28, 471
Fsp4HI	GCNGC	1 cut(s)	166
FspBI	CTAG	1 cut(s)	144
GluI	GCNGC	1 cut(s)	166
GsuI	CTGGAG	1 cut(s)	845
HaeIII	GGCC	4 cut(s)	184, 571, 642, 804
HapII	CCGG	5 cut(s)	75, 317, 572, 796, 922
HgaI	GACGC	1 cut(s)	157
Hin1II	CATG	4 cut(s)	266, 401, 803, 979
HinfI	GANTC	2 cut(s)	588, 851
HpaII	CCGG	5 cut(s)	75, 317, 572, 796, 922
HphI	GGTGA	2 cut(s)	426, 509
Hpy166II	GTNNAC	2 cut(s)	375, 649
Hpy188I	TCNGA	1 cut(s)	1019
Hpy188III	TCNNGA	2 cut(s)	311, 425
Hpy8I	GTNNAC	2 cut(s)	375, 649
HpyAV	CCTTC	4 cut(s)	123, 457, 488, 667
HpyCH4III	ACNGT	2 cut(s)	346, 785
HpyCH4IV	ACGT	1 cut(s)	684
HpyCH4V	TGCA	3 cut(s)	165, 483, 944
HpyF10VI	GCNNNNNNNGC	1 cut(s)	801
HpyF3I	CTNAG	3 cut(s)	192, 288, 456
HpySE526I	ACGT	1 cut(s)	684
Hsp92II	CATG	4 cut(s)	266, 401, 803, 979
KflI	GGGWCCC	1 cut(s)	155
KpnI	GGTACC	1 cut(s)	784
KroI	GCCGGC	2 cut(s)	316, 795
KroNI	GCCGGC	2 cut(s)	318, 797
Ksp22I	TGATCA	1 cut(s)	259
Kzo9I	GATC	6 cut(s)	259, 322, 508, 574, 981, 988
LguI	GCTCTTC	1 cut(s)	701
LmnI	GCTCC	3 cut(s)	466, 935, 947
Lsp1109I	GCAGC	1 cut(s)	177
LweI	GCATC	4 cut(s)	295, 394, 490, 681
MaeI	CTAG	1 cut(s)	144
MaeII	ACGT	1 cut(s)	684
MaeIII	GTNAC	2 cut(s)	415, 823
MalI	GATC	6 cut(s)	261, 324, 510, 576, 983, 990
MboI	GATC	6 cut(s)	259, 322, 508, 574, 981, 988
MboII	GAAGA	2 cut(s)	688, 722
MflI	RGATCY	2 cut(s)	508, 574
MhlI	GDGCHC	2 cut(s)	28, 471
MluCI	AATT	6 cut(s)	82, 133, 420, 765, 927, 993
MmeI	TCCRAC	1 cut(s)	337
MroNI	GCCGGC	2 cut(s)	316, 795
MroXI	GAANNNNTTC	1 cut(s)	133
MseI	TTAA	2 cut(s)	753, 842
MslI	CAYNNNNRTG	2 cut(s)	402, 974
MspCI	CTTAAG	1 cut(s)	841
MspI	CCGG	5 cut(s)	75, 317, 572, 796, 922
MspR9I	CCNGG	2 cut(s)	76, 276
MvaI	CCWGG	1 cut(s)	276
MwoI	GCNNNNNNNGC	1 cut(s)	801
NaeI	GCCGGC	2 cut(s)	318, 797
NciI	CCSGG	1 cut(s)	76
NdeI	CATATG	1 cut(s)	161
NdeII	GATC	6 cut(s)	259, 322, 508, 574, 981, 988
NgoMIV	GCCGGC	2 cut(s)	316, 795
NlaIII	CATG	4 cut(s)	266, 401, 803, 979
NlaIV	GGNNCC	8 cut(s)	73, 156, 157, 468, 576, 728, 782, 966
NmeAIII	GCCGAG	2 cut(s)	222, 529
NmuCI	GTSAC	1 cut(s)	823
NspI	RCATGY	2 cut(s)	401, 979
PaeR7I	CTCGAG	1 cut(s)	311
PasI	CCCWGGG	1 cut(s)	275
PceI	AGGCCT	1 cut(s)	184
PciI	ACATGT	1 cut(s)	975
PciSI	GCTCTTC	1 cut(s)	701
PcsI	WCGNNNNNNNCGW	1 cut(s)	309
PdiI	GCCGGC	2 cut(s)	318, 797
PdmI	GAANNNNTTC	1 cut(s)	133
PfeI	GAWTC	2 cut(s)	588, 851
PflMI	CCANNNNNTGG	1 cut(s)	584
PkrI	GCNGC	1 cut(s)	167
PpuMI	RGGWCCY	1 cut(s)	155
PscI	ACATGT	1 cut(s)	975
Psp5II	RGGWCCY	1 cut(s)	155
Psp6I	CCWGG	1 cut(s)	274
PspGI	CCWGG	1 cut(s)	274
PspN4I	GGNNCC	8 cut(s)	73, 156, 157, 468, 576, 728, 782, 966
PspPI	GGNCC	4 cut(s)	155, 641, 770, 869
PspPPI	RGGWCCY	1 cut(s)	155
PstI	CTGCAG	1 cut(s)	946
PsuI	RGATCY	2 cut(s)	508, 574
RsaI	GTAC	4 cut(s)	566, 609, 650, 782
RsaNI	GTAC	4 cut(s)	565, 608, 649, 781
RseI	CAYNNNNRTG	2 cut(s)	402, 974
SapI	GCTCTTC	1 cut(s)	701
SaqAI	TTAA	2 cut(s)	753, 842
SatI	GCNGC	1 cut(s)	166
Sau3AI	GATC	6 cut(s)	259, 322, 508, 574, 981, 988
Sau96I	GGNCC	4 cut(s)	155, 641, 770, 869
ScaI	AGTACT	1 cut(s)	566
ScrFI	CCNGG	2 cut(s)	76, 276
SduI	GDGCHC	2 cut(s)	28, 471
SfaNI	GCATC	4 cut(s)	295, 394, 490, 681
SfcI	CTRYAG	3 cut(s)	235, 249, 942
Sfr274I	CTCGAG	1 cut(s)	311
SinI	GGWCC	3 cut(s)	155, 770, 869
SlaI	CTCGAG	1 cut(s)	311
SmiMI	CAYNNNNRTG	2 cut(s)	402, 974
SmlI	CTYRAG	3 cut(s)	178, 311, 841
SmoI	CTYRAG	3 cut(s)	178, 311, 841
Sse9I	AATT	6 cut(s)	82, 133, 420, 765, 927, 993
SseBI	AGGCCT	1 cut(s)	184
SsiI	CCGC	2 cut(s)	437, 533
SspMI	CTAG	1 cut(s)	144
StuI	AGGCCT	1 cut(s)	184
StyD4I	CCNGG	2 cut(s)	74, 274
StyI	CCWWGG	2 cut(s)	138, 450
TaaI	ACNGT	2 cut(s)	346, 785
TaiI	ACGT	1 cut(s)	687
TaqI	TCGA	2 cut(s)	312, 492
TasI	AATT	6 cut(s)	82, 133, 420, 765, 927, 993
TatI	WGTACW	1 cut(s)	564
TfiI	GAWTC	2 cut(s)	588, 851
Tru1I	TTAA	2 cut(s)	753, 842
Tru9I	TTAA	2 cut(s)	753, 842
TscAI	CASTG	2 cut(s)	351, 387
TseFI	GTSAC	1 cut(s)	823
TseI	GCWGC	1 cut(s)	165
Tsp45I	GTSAC	1 cut(s)	823
TspDTI	ATGAA	1 cut(s)	50
TspRI	CASTG	2 cut(s)	351, 387
Van91I	CCANNNNNTGG	1 cut(s)	584
Vha464I	CTTAAG	1 cut(s)	841
VpaK11BI	GGWCC	3 cut(s)	155, 770, 869
XapI	RAATTY	2 cut(s)	82, 133
XceI	RCATGY	2 cut(s)	401, 979
XhoI	CTCGAG	1 cut(s)	311
XmnI	GAANNNNTTC	1 cut(s)	133
XspI	CTAG	1 cut(s)	144
ZrmI	AGTACT	1 cut(s)	566

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.