Rorug04G0203300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001509
Physical Location & Seq
Forward (+)
35360133 .. 35360520
388 bp
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UTR
Exon/CDS
Intron
Rorug04G0203300.1

Sequence Viewer

Length: 294 bp
ATGGAGTCGATCCCGGCCGGCGAGAAGTACATGCAGATTAAGAAGAAGCTCAGGGAATTGAAGCTCCACACAGTCTGCGAGGAGGCCAAGTGTCCCAATCTCGGCGAGTGCTGGTCCGGCGGAGGTTTACGGCGGCGAAACCGCCACTGCCACCACCACTATCATGATTCTTGGCAACAAGTGTACGCGCGGTTGCAGACTTCTAGGACTCCGCCGCTGCTGGACCCCAATGAGCCGGCGAATGTGGAGGAGGCGATTGCGTCGTGGGGGTTGGATTGTGTGGTGAATACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

97

Amino Acids

11.16

Weight (kDa)

7.7

Isoelectric Point (pI)

71.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LIAS_N PF16881 3 - 31 1.5e-10 N-terminal domain of lipoyl synthase of Radical_SAM family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 188, 190
AciI CCGC 6 cut(s) 120, 133, 142, 190, 212, 215
AclWI GGATC 1 cut(s) 4
AcoI YGGCCR 1 cut(s) 15
AfaI GTAC 2 cut(s) 29, 185
AfiI CCNNNNNNNGG 1 cut(s) 101
AgsI TTSAA 1 cut(s) 61
AluBI AGCT 2 cut(s) 49, 64
AluI AGCT 2 cut(s) 49, 64
AlwI GGATC 1 cut(s) 4
AoxI GGCC 2 cut(s) 15, 84
ApeKI GCWGC 1 cut(s) 217
AspLEI GCGC 1 cut(s) 190
AspS9I GGNCC 2 cut(s) 114, 223
AsuC2I CCSGG 1 cut(s) 14
AvaII GGWCC 2 cut(s) 114, 223
BbvI GCAGC 1 cut(s) 204
BceAI ACGGC 1 cut(s) 146
BcnI CCSGG 1 cut(s) 14
BfaI CTAG 1 cut(s) 204
BisI GCNGC 3 cut(s) 134, 215, 218
BlsI GCNGC 3 cut(s) 135, 216, 219
Bme1390I CCNGG 1 cut(s) 14
Bme18I GGWCC 2 cut(s) 114, 223
BmgT120I GGNCC 2 cut(s) 114, 223
BmiI GGNNCC 1 cut(s) 225
BmrFI CCNGG 1 cut(s) 14
Bpu10I CCTNAGC 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 14
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 1 cut(s) 101
Bse118I RCCGGY 2 cut(s) 17, 235
BseLI CCNNNNNNNGG 1 cut(s) 101
BseMII CTCAG 1 cut(s) 64
BseRI GAGGAG 2 cut(s) 95, 263
BseX3I CGGCCG 1 cut(s) 15
BseXI GCAGC 1 cut(s) 204
Bsh1236I CGCG 2 cut(s) 188, 190
Bsh1285I CGRYCG 1 cut(s) 18
BshFI GGCC 2 cut(s) 17, 86
BsiEI CGRYCG 1 cut(s) 18
BsiSI CCGG 4 cut(s) 14, 18, 117, 236
BslFI GGGAC 1 cut(s) 78
BslI CCNNNNNNNGG 1 cut(s) 101
BsmFI GGGAC 1 cut(s) 78
BsnI GGCC 2 cut(s) 17, 86
Bsp143I GATC 1 cut(s) 9
BspACI CCGC 6 cut(s) 120, 133, 142, 190, 212, 215
BspANI GGCC 2 cut(s) 17, 86
BspCNI CTCAG 1 cut(s) 63
BspFNI CGCG 2 cut(s) 188, 190
BspHI TCATGA 1 cut(s) 163
BspLI GGNNCC 1 cut(s) 225
BspPI GGATC 1 cut(s) 4
BsrFI RCCGGY 2 cut(s) 17, 235
BssAI RCCGGY 2 cut(s) 17, 235
BssMI GATC 1 cut(s) 9
Bst4CI ACNGT 1 cut(s) 73
BstC8I GCNNGC 2 cut(s) 19, 237
BstDEI CTNAG 1 cut(s) 50
BstFNI CGCG 2 cut(s) 188, 190
BstHHI GCGC 1 cut(s) 190
BstKTI GATC 1 cut(s) 12
BstMBI GATC 1 cut(s) 9
BstMCI CGRYCG 1 cut(s) 18
BstMWI GCNNNNNNNGC 1 cut(s) 117
BstNSI RCATGY 1 cut(s) 34
BstSCI CCNGG 1 cut(s) 12
BstUI CGCG 2 cut(s) 188, 190
BstV1I GCAGC 1 cut(s) 204
BstZI CGGCCG 1 cut(s) 15
BsuRI GGCC 2 cut(s) 17, 86
BtsI GCAGTG 1 cut(s) 145
BtsIMutI CAGTG 1 cut(s) 145
Cac8I GCNNGC 2 cut(s) 19, 237
CciI TCATGA 1 cut(s) 163
CfoI GCGC 1 cut(s) 190
Cfr10I RCCGGY 2 cut(s) 17, 235
Cfr13I GGNCC 2 cut(s) 114, 223
CseI GACGC 1 cut(s) 249
Csp6I GTAC 2 cut(s) 28, 184
CviAII CATG 2 cut(s) 31, 164
CviJI RGCY 5 cut(s) 17, 49, 64, 86, 235
CviKI_1 RGCY 5 cut(s) 17, 49, 64, 86, 235
CviQI GTAC 2 cut(s) 28, 184
DdeI CTNAG 1 cut(s) 50
DpnI GATC 1 cut(s) 11
DpnII GATC 1 cut(s) 9
EaeI YGGCCR 1 cut(s) 15
EagI CGGCCG 1 cut(s) 15
EciI GGCGGA 2 cut(s) 135, 201
EclXI CGGCCG 1 cut(s) 15
Eco47I GGWCC 2 cut(s) 114, 223
Eco52I CGGCCG 1 cut(s) 15
FaeI CATG 2 cut(s) 34, 167
FaiI YATR 2 cut(s) 32, 165
FaqI GGGAC 1 cut(s) 78
FatI CATG 2 cut(s) 30, 163
Fnu4HI GCNGC 3 cut(s) 134, 215, 218
Fsp4HI GCNGC 3 cut(s) 134, 215, 218
FspBI CTAG 1 cut(s) 204
GlaI GCGC 1 cut(s) 189
GluI GCNGC 3 cut(s) 134, 215, 218
HaeIII GGCC 2 cut(s) 17, 86
HapII CCGG 4 cut(s) 14, 18, 117, 236
HgaI GACGC 1 cut(s) 249
HhaI GCGC 1 cut(s) 190
Hin1II CATG 2 cut(s) 34, 167
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HinfI GANTC 3 cut(s) 5, 167, 208
HpaII CCGG 4 cut(s) 14, 18, 117, 236
Hpy166II GTNNAC 2 cut(s) 128, 184
Hpy188III TCNNGA 1 cut(s) 164
Hpy8I GTNNAC 2 cut(s) 128, 184
Hpy99I CGWCG 1 cut(s) 265
HpyCH4III ACNGT 1 cut(s) 73
HpyCH4V TGCA 2 cut(s) 34, 196
HpyF10VI GCNNNNNNNGC 1 cut(s) 117
HpyF3I CTNAG 1 cut(s) 50
Hsp92II CATG 2 cut(s) 34, 167
HspAI GCGC 1 cut(s) 188
KroI GCCGGC 2 cut(s) 17, 235
KroNI GCCGGC 2 cut(s) 19, 237
Kzo9I GATC 1 cut(s) 9
LmnI GCTCC 1 cut(s) 69
LpnPI CCDG 7 cut(s) 27, 31, 37, 97, 130, 206, 249
Lsp1109I GCAGC 1 cut(s) 204
MaeI CTAG 1 cut(s) 204
MalI GATC 1 cut(s) 11
MboI GATC 1 cut(s) 9
MboII GAAGA 1 cut(s) 55
MluCI AATT 1 cut(s) 56
MlyI GAGTC 2 cut(s) 14, 202
MmeI TCCRAC 1 cut(s) 252
MnlI CCTC 5 cut(s) 73, 76, 116, 241, 244
MroNI GCCGGC 2 cut(s) 17, 235
MseI TTAA 1 cut(s) 39
MslI CAYNNNNRTG 1 cut(s) 162
MspA1I CMGCKG 1 cut(s) 217
MspI CCGG 4 cut(s) 14, 18, 117, 236
MspR9I CCNGG 1 cut(s) 14
MvnI CGCG 2 cut(s) 188, 190
MwoI GCNNNNNNNGC 1 cut(s) 117
NaeI GCCGGC 2 cut(s) 19, 237
NciI CCSGG 1 cut(s) 14
NdeII GATC 1 cut(s) 9
NgoMIV GCCGGC 2 cut(s) 17, 235
NlaIII CATG 2 cut(s) 34, 167
NlaIV GGNNCC 1 cut(s) 225
NmeAIII GCCGAG 1 cut(s) 81
NspI RCATGY 1 cut(s) 34
PagI TCATGA 1 cut(s) 163
PdiI GCCGGC 2 cut(s) 19, 237
PfeI GAWTC 1 cut(s) 167
PkrI GCNGC 3 cut(s) 135, 216, 219
PleI GAGTC 2 cut(s) 13, 202
PpsI GAGTC 2 cut(s) 13, 202
PspN4I GGNNCC 1 cut(s) 225
PspPI GGNCC 2 cut(s) 114, 223
RsaI GTAC 2 cut(s) 29, 185
RsaNI GTAC 2 cut(s) 28, 184
RseI CAYNNNNRTG 1 cut(s) 162
SaqAI TTAA 1 cut(s) 39
SatI GCNGC 3 cut(s) 134, 215, 218
Sau3AI GATC 1 cut(s) 9
Sau96I GGNCC 2 cut(s) 114, 223
SchI GAGTC 2 cut(s) 14, 202
ScrFI CCNGG 1 cut(s) 14
SetI ASST 3 cut(s) 51, 66, 127
SinI GGWCC 2 cut(s) 114, 223
SmiMI CAYNNNNRTG 1 cut(s) 162
Sse9I AATT 1 cut(s) 56
SsiI CCGC 6 cut(s) 120, 133, 142, 190, 212, 215
SspMI CTAG 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 12
TaaI ACNGT 1 cut(s) 73
TaqI TCGA 1 cut(s) 8
TasI AATT 1 cut(s) 56
TatI WGTACW 1 cut(s) 27
TauI GCSGC 2 cut(s) 136, 217
TfiI GAWTC 1 cut(s) 167
Tru1I TTAA 1 cut(s) 39
Tru9I TTAA 1 cut(s) 39
TscAI CASTG 1 cut(s) 152
TseI GCWGC 1 cut(s) 217
TspRI CASTG 1 cut(s) 152
VpaK11BI GGWCC 2 cut(s) 114, 223
XceI RCATGY 1 cut(s) 34
XspI CTAG 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.