Rorug04G0272200

MATE efflux family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005868
Physical Location & Seq
Reverse (-)
44472655 .. 44473558
904 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0272200.1

Sequence Viewer

Length: 558 bp
ATGATAAAAACTTTGAAAATTCTTTTTAAATTGGATTTCCATCTGTATATATATGTACTGCCACTGGTTAGTTTCCACCGGTTCCTATATATAATCAAGGAGTTATTGTGCCCGATTGCCCCATCGCGCATGTTCAAGGCTTTGATCATAGACTCCAAAAATTTGATCCCAAAGCTGTTGCCCCAATTCATAGCAAGCGTTGAAGTAACTCAAGGAGATGGCGGAGCAGGAAGCATTGAACAAGTCAACTTCACAGAAGGTAGCCATTTCAAATACGTCAAGCACAGAATCGATGAACTCGACCAGGACAATTTCGTGTGCAAGTACACTATGATTGAGGGAGATGCATTAGGGGACAAGCTTGAGTCTATTGCTTACGAGGTCAAGTTTGAGGCAGCTAGTGATGGGAGCTCTATATGCAAGATGACAAGTAATTACAACACCATTGGAGAGTTTGAGGTCAAAGAAGAGGAGATTATAGCAGGAAAGAATAGTGCTATGGGAATTTACAAAGTTGTTGAAGCCTACCTCTTAGAGAACCCAAATGTTTATTCTTGA

Protein Analysis

185

Amino Acids

20.94

Weight (kDa)

5.26

Isoelectric Point (pI)

20.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 34 - 180 1.6e-23 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 127
AciI CCGC 1 cut(s) 222
AclWI GGATC 1 cut(s) 160
AcsI RAATTY 3 cut(s) 18, 160, 504
AfaI GTAC 2 cut(s) 57, 326
AgeI ACCGGT 1 cut(s) 78
AgsI TTSAA 6 cut(s) 16, 136, 203, 239, 271, 521
AjnI CCWGG 1 cut(s) 303
AjuI GAANNNNNNNTTGG 1 cut(s) 535
AluBI AGCT 4 cut(s) 175, 361, 398, 411
AluI AGCT 4 cut(s) 175, 361, 398, 411
Alw21I GWGCWC 1 cut(s) 413
AlwI GGATC 1 cut(s) 160
ApeKI GCWGC 1 cut(s) 395
ApoI RAATTY 3 cut(s) 18, 160, 504
AsiGI ACCGGT 1 cut(s) 78
AspLEI GCGC 1 cut(s) 129
BaeGI GKGCMC 1 cut(s) 113
BanII GRGCYC 1 cut(s) 413
Bbv12I GWGCWC 1 cut(s) 413
BbvI GCAGC 1 cut(s) 407
BccI CCATC 4 cut(s) 48, 130, 212, 398
BciT130I CCWGG 1 cut(s) 305
BclI TGATCA 1 cut(s) 144
BfaI CTAG 1 cut(s) 399
BisI GCNGC 1 cut(s) 396
BlsI GCNGC 1 cut(s) 397
Bme1390I CCNGG 1 cut(s) 305
BmiI GGNNCC 1 cut(s) 83
BmrFI CCNGG 1 cut(s) 305
BmsI GCATC 1 cut(s) 334
BpuEI CTTGAG 2 cut(s) 195, 383
Bsa29I ATCGAT 1 cut(s) 291
BsaBI GATNNNNATC 1 cut(s) 39
BsaWI WCCGGW 1 cut(s) 78
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bse118I RCCGGY 1 cut(s) 78
Bse1I ACTGG 1 cut(s) 69
Bse8I GATNNNNATC 1 cut(s) 39
BseBI CCWGG 1 cut(s) 305
BseCI ATCGAT 1 cut(s) 291
BseJI GATNNNNATC 1 cut(s) 39
BseNI ACTGG 1 cut(s) 69
BseRI GAGGAG 1 cut(s) 485
BseSI GKGCMC 1 cut(s) 113
BseXI GCAGC 1 cut(s) 407
Bsh1236I CGCG 1 cut(s) 127
BshTI ACCGGT 1 cut(s) 78
BshVI ATCGAT 1 cut(s) 291
BsiHKAI GWGCWC 1 cut(s) 413
BsiSI CCGG 1 cut(s) 79
BslFI GGGAC 1 cut(s) 368
BsmFI GGGAC 1 cut(s) 368
Bsp1286I GDGCHC 2 cut(s) 113, 413
Bsp143I GATC 2 cut(s) 144, 165
BspACI CCGC 1 cut(s) 222
BspDI ATCGAT 1 cut(s) 291
BspFNI CGCG 1 cut(s) 127
BspLI GGNNCC 1 cut(s) 83
BspPI GGATC 1 cut(s) 160
BsrFI RCCGGY 1 cut(s) 78
BsrI ACTGG 1 cut(s) 69
BssAI RCCGGY 1 cut(s) 78
BssMI GATC 2 cut(s) 144, 165
Bst2UI CCWGG 1 cut(s) 305
Bst6I CTCTTC 1 cut(s) 462
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 1 cut(s) 532
BstFNI CGCG 1 cut(s) 127
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 2 cut(s) 147, 168
BstMBI GATC 2 cut(s) 144, 165
BstMWI GCNNNNNNNGC 1 cut(s) 417
BstNI CCWGG 1 cut(s) 305
BstNSI RCATGY 1 cut(s) 133
BstSCI CCNGG 1 cut(s) 303
BstSLI GKGCMC 1 cut(s) 113
BstUI CGCG 1 cut(s) 127
BstV1I GCAGC 1 cut(s) 407
Bsu15I ATCGAT 1 cut(s) 291
BsuTUI ATCGAT 1 cut(s) 291
BtgZI GCGATG 1 cut(s) 108
BtsIMutI CAGTG 1 cut(s) 62
Cac8I GCNNGC 1 cut(s) 196
CfoI GCGC 1 cut(s) 129
Cfr10I RCCGGY 1 cut(s) 78
ClaI ATCGAT 1 cut(s) 291
Csp6I GTAC 2 cut(s) 56, 325
CspAI ACCGGT 1 cut(s) 78
CviAII CATG 1 cut(s) 130
CviJI RGCY 7 cut(s) 140, 175, 264, 361, 398, 411, 524
CviKI_1 RGCY 7 cut(s) 140, 175, 264, 361, 398, 411, 524
CviQI GTAC 2 cut(s) 56, 325
DdeI CTNAG 1 cut(s) 532
DpnI GATC 2 cut(s) 146, 167
DpnII GATC 2 cut(s) 144, 165
DraI TTTAAA 1 cut(s) 28
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
EciI GGCGGA 1 cut(s) 237
Ecl136II GAGCTC 1 cut(s) 411
Eco24I GRGCYC 1 cut(s) 413
Eco53kI GAGCTC 1 cut(s) 411
EcoICRI GAGCTC 1 cut(s) 411
EcoRII CCWGG 1 cut(s) 303
EcoT22I ATGCAT 1 cut(s) 349
EcoT38I GRGCYC 1 cut(s) 413
FaeI CATG 1 cut(s) 133
FaqI GGGAC 1 cut(s) 368
FatI CATG 1 cut(s) 129
FbaI TGATCA 1 cut(s) 144
Fnu4HI GCNGC 1 cut(s) 396
FriOI GRGCYC 1 cut(s) 413
Fsp4HI GCNGC 1 cut(s) 396
FspBI CTAG 1 cut(s) 399
GlaI GCGC 1 cut(s) 128
GluI GCNGC 1 cut(s) 396
HapII CCGG 1 cut(s) 79
HhaI GCGC 1 cut(s) 129
Hin1II CATG 1 cut(s) 133
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HindIII AAGCTT 1 cut(s) 359
HinfI GANTC 3 cut(s) 152, 288, 365
HpaII CCGG 1 cut(s) 79
Hpy166II GTNNAC 2 cut(s) 247, 327
Hpy188III TCNNGA 1 cut(s) 555
Hpy8I GTNNAC 2 cut(s) 247, 327
HpyAV CCTTC 1 cut(s) 251
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 3 cut(s) 321, 347, 420
HpyF10VI GCNNNNNNNGC 1 cut(s) 417
HpyF3I CTNAG 1 cut(s) 532
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 1 cut(s) 133
HspAI GCGC 1 cut(s) 127
Ksp22I TGATCA 1 cut(s) 144
Kzo9I GATC 2 cut(s) 144, 165
LmnI GCTCC 2 cut(s) 224, 408
LpnPI CCDG 6 cut(s) 50, 92, 213, 290, 317, 468
Lsp1109I GCAGC 1 cut(s) 407
LweI GCATC 1 cut(s) 334
MaeI CTAG 1 cut(s) 399
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 1 cut(s) 205
MalI GATC 2 cut(s) 146, 167
MboI GATC 2 cut(s) 144, 165
MboII GAAGA 1 cut(s) 479
MhlI GDGCHC 2 cut(s) 113, 413
MluCI AATT 7 cut(s) 18, 29, 160, 185, 310, 433, 504
MlyI GAGTC 2 cut(s) 146, 374
MnlI CCTC 6 cut(s) 331, 373, 385, 451, 463, 539
Mph1103I ATGCAT 1 cut(s) 349
MseI TTAA 1 cut(s) 27
MspI CCGG 1 cut(s) 79
MspR9I CCNGG 1 cut(s) 305
MvaI CCWGG 1 cut(s) 305
MvnI CGCG 1 cut(s) 127
MwoI GCNNNNNNNGC 1 cut(s) 417
NdeII GATC 2 cut(s) 144, 165
NlaIII CATG 1 cut(s) 133
NlaIV GGNNCC 1 cut(s) 83
NsiI ATGCAT 1 cut(s) 349
NspI RCATGY 1 cut(s) 133
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PfeI GAWTC 1 cut(s) 288
PinAI ACCGGT 1 cut(s) 78
PkrI GCNGC 1 cut(s) 397
PleI GAGTC 2 cut(s) 146, 373
PpsI GAGTC 2 cut(s) 146, 373
Psp124BI GAGCTC 1 cut(s) 413
Psp6I CCWGG 1 cut(s) 303
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 1 cut(s) 83
RsaI GTAC 2 cut(s) 57, 326
RsaNI GTAC 2 cut(s) 56, 325
SacI GAGCTC 1 cut(s) 413
SaqAI TTAA 1 cut(s) 27
SatI GCNGC 1 cut(s) 396
Sau3AI GATC 2 cut(s) 144, 165
SchI GAGTC 2 cut(s) 146, 374
ScrFI CCNGG 1 cut(s) 305
SduI GDGCHC 2 cut(s) 113, 413
SetI ASST 9 cut(s) 177, 262, 279, 363, 384, 400, 413, 462, 531
SfaNI GCATC 1 cut(s) 334
SmlI CTYRAG 2 cut(s) 210, 362
SmoI CTYRAG 2 cut(s) 210, 362
Sse9I AATT 7 cut(s) 18, 29, 160, 185, 310, 433, 504
SsiI CCGC 1 cut(s) 222
SspMI CTAG 1 cut(s) 399
SstI GAGCTC 1 cut(s) 413
StyD4I CCNGG 1 cut(s) 303
TaiI ACGT 1 cut(s) 279
TaqI TCGA 2 cut(s) 291, 300
TasI AATT 7 cut(s) 18, 29, 160, 185, 310, 433, 504
TatI WGTACW 2 cut(s) 55, 324
TfiI GAWTC 1 cut(s) 288
Tru1I TTAA 1 cut(s) 27
Tru9I TTAA 1 cut(s) 27
TscAI CASTG 1 cut(s) 69
TseI GCWGC 1 cut(s) 395
TspDTI ATGAA 2 cut(s) 178, 309
TspRI CASTG 1 cut(s) 69
XapI RAATTY 3 cut(s) 18, 160, 504
XceI RCATGY 1 cut(s) 133
XspI CTAG 1 cut(s) 399
Zsp2I ATGCAT 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.