Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0001263

Physical Location & Seq

Genomic Coordinates Forward (+)

60000140 .. 60008955

Gene Structure 8816 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0444000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2742 bp

ATGGAGTTGAGGCTCATCCATGGAGTAGCATACAGTGAGCCCTGGTTTGGTCGTTGGGGTTACAAATTTGGCCGTGGAACATTTTGTGTCACTCAACAAATGTACCAAAGCGCCATTGAAGCATTACAAGGCATGCCCTTGTGCTTACTCATCCACCATCTTGCTGACATTACTTGCAATCAATTAGGCCACGATATTTCCCTCATCTTCTCAAGGTATCACGCATTGTCGGATCATTCATTGGCGACTCTCGGTGACCTATTCCATTTCATGCTAGAGCTCAAGTCCCGGTTACCCACTGACGATCAAAACTCGTGCATTGATTCCTATAACCCGGGAATGCTGGTGGAGACATCTTGCAGATGGTCTCCTAAAAGAGTTGAAATGGCGTCTCGGGTGATTGTGGAAGCCTTAAAGAGGGCTCATTTCAGATGGGTCTCTAGGCAGGAAGTAAGGGATGCTGCTCGCTCGTATATAGGGGACACGGGCTTGCTAGACTTCGTGTTGAAGTCGTTGGGCAACCAAATTGTCGGGAATTACTTGGTTCGTCGCAGCTTGAATCCGGTGACCAAAGTTCTCGAGTATTGCTTAGAAGACCTTTCTAATGTGTCCCCTAACCAAGATGGCAATGTCATGACCAACTCCAAAGTGAAGGGATGGTACAAGATTTCGAGGTTCCAGCTAATGAAGGACATGTTCTACTTGTACAAGTACATCCTCAAAGACCAAAAGCTAAGCACCGGTACTACTACAGGGATCCTTTCAGCAATTCCGATGGCTGTTAGGATCATTCTTGATTCCAAGTACCTTGTCAAAGATTACTGCGGCGAGTTACCGCATGTGAAACTCGAATTAGGACCTGAAGGAAAATCAAATCTCTATTGTACCGTAATGCTAAGAAACAGTACACAAGTGCTTGATTATAACAAGATGATTAACAACAACGAATTATTATTGCCGCCATACGAGTGTGTGACACTGAAAAACAATGCTACCACCAATGATCTTAAGCTCGAAGTGGAGAGGAACTTCAAAGAGATCTACTGGGGATTGAAAAGCTTCGTCGTGGAAACGATTGCGAATTTGAGTATCGATGCAAAGGGAACAGATATGGTTTTTGGGCTGGTTGAGGTGGGTGGAAAGATTGTGCTTAAAGGAAGCAACAAAGATATGAGAGGTGGTGGACTTGGACTTGTTAGAGAGCATGAAGGACCCATATTAGAATGCGGGTCGGTTAACTGCATTGTGGACTGTCCATGTGGTGCCAAAGATGACGACGGGGAAAGAATGTGGACTTTCACAACAAACTCATTCCACCCTATGTTGGCCAACAACAACCACACCAACACTACTTGTGCCTCCTCTGCTGCTCCTTATTCTTCCTCTGATCATCATCATGCCTTCACTCCCTTAGAAAATGGAGCAGCAGCAGCAGCGACAGCCACAAACAAAAGAAAACGCCGACCGGCCGGCACCCCAGATCCGGATGCGGAAGTGGTGTCTCTCTCCCCGAAAACGTTACTGGAGTCAGACCGTTACGTGTGCGAGATCTGTAACCAGGGGTTCCAGAGAGACCAGAATCTGCAGATGCACAGAAGAAGGCACAAGGTGCCGTGGAAGTTGCTGAAGCGGGAAATACAGGATCAGGTGAAGAAGAGGGTGTTTGTGTGCCCGGAGCCGAGCTGCCTCCACCACGACCCTTGCCATGCGCTCGGCGATCTCGTCGGAATCAAGAAGCACTTCCGGCGAAAGCACAGCAACCACAAGCAATGGGTGTGCGACAAGTGCTCCAAAGGTTACGCCGTACAATCCGATTACAAGGCCCATCTCAAAACCTGTGGCACCAGAGGACATTCCTGCGACTGTGGCCGTGTGTTTTCCAGAGTGGAGAGTTTCATTGAGCACCAAGATACTTGCAGCGTGCGACATGTTGTCCGGCCAGAATTGCAGGCACTTCAGCCGGCGGCGGCCTGCTCATCACGAACAGCGTCGAGCACCAGCCCCTCCAGCGACGGCCATTTCAGCATTAATAATAACAACAATGCAGCTCCTCCATTGCCCGGATTACGAGTAATCCCAAAGCCAAATGACCAACCTGTTGTGTTTATTAGCTCTCATGAGGGGCATGATGGTAATCCTTCCACTTCTAACCACCAACAGCCACGAGAACAAGAACAAGAGCATATGCTGGAACTTAAACTCCTTCCATCGTCGGACACACAAACCTCCCCTCGAAACGAAGACGAAAACTATGCAACGCATTTGAAGCTTTCCATAGGCGGCTCATCACGCGGTGGTGATCAAAAGAATGATTCGCCACGAAGCTCATCAAGAGAAATGAACACAAACATTGGTTTTGGTGGTAGAGCAAGTCGGGAAGTGGCGAGGTTGAAAGACTTTGCCTCCGAGGAGTTGAAGTTGGCCATGGCGGAAAAGGCCTACGCCGAAGACGCCAGACGAGAAGCCAAGCGGCAAATAGAGATGGCCGAGCTGGAGTTCGCAAACGCAAAGAGGATCAGGCAACAAGCGCAGGCGGAGCTTCAAAAGGCTCAGCTACTAAAAGAGCAATCCACCAAGAAAATCAGCTCCGCCATTATGCAAATCACTTGTCCATCTTGCAAGCAACACTTCCATGCGAGTAATCCTATTAACGCAGTCGGAGTGGGACCCTCCGACGAGACGACCTCCCTCGGCATGAGTTACATGTCCTCCGCCACAACCGAAGGAGAAGGAGAACAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0002376 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009409 GO:0009414 GO:0009415 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009631 GO:0009648 GO:0009791 GO:0009814 GO:0009816 GO:0009889 GO:0009891 GO:0009893 GO:0010035 GO:0010228 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0042221 GO:0042742 GO:0043207 GO:0043565 GO:0045087 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0048571 GO:0048573 GO:0048574 GO:0048578 GO:0048580 GO:0048582 GO:0048583 GO:0048584 GO:0048586 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0097159 GO:0098542 GO:1901363 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000026 GO:2000028 GO:2000112 GO:2000241 GO:2000243 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

913

Amino Acids

102.05

Weight (kDa)

8.37

Isoelectric Point (pI)

47.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHD_plant_repro	PF25874	121 - 199	5.3e-34	Winged helix-turn-helix domain in plant reproductive proteins
Ubiquitin_At1g33420	PF25565	289 - 387	5.8e-27	At1g33420-like, ubiquitin-like domain
C2H2-2nd_BIRD-IDD	PF22996	551 - 585	1e-17	BIRD-IDD transcription factor, second C2H2 zinc finger
C2CH-3rd_BIRD-IDD	PF22995	590 - 614	8.7e-14	BIRD-IDD transcription factor, third C2HC zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001263)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28520 AT1G28520 AT1G28520 AT1G28520 AT1G28520
fragaria_vesca	FvH4_3g08390 FvH4_3g08390 FvH4_3g08390 FvH4_3g08390 FvH4_3g08390 FvH4_3g08390 FvH4_6g26850 FvH4_6g26850 FvH4_6g26850 FvH4_6g26850 FvH4_6g26850 FvH4_6g26850 FvH4_6g26850
malus_domestica	MD05G1308300.v1.1 MD10G1287300.v1.1 MD17G1234200.v1.1
prunus_persica	Prupe.3G127200_v2.0.a1 Prupe.3G127200_v2.0.a1 Prupe.3G127200_v2.0.a1 Prupe.4G054500_v2.0.a1 Prupe.4G054500_v2.0.a1
pyrus_communis	pycom05g28500 pycom10g24030 pycom17g23750
rosa_chinensis	RchiOBHm_Chr2g0128221 RchiOBHm_Chr5g0009511
rosa_laevigata	RLG00000018998 RLG00000031668
rosa_multiflora	Rmu_sc0000837.1_g000011 Rmu_sc0002711.1_g000012 Rmu_sc0003465.1_g000003
rosa_roxburghii	Rroxscaffold_1G00066620 Rroxscaffold_1G00067320 Rroxscaffold_2G00115480 Rroxscaffold_2G00115940
rosa_rugosa	Rorug02G0278700 Rorug04G0443800 Rorug04G0443800 Rorug04G0443900 Rorug04G0444000
rosa_samantha	Rh2AG331400 Rh2BG339600 Rh2CG317900 Rh2DG356500 Rh5AG074800 Rh5BG070600 Rh5CG083000 Rh5DG070800
rosa_wichuraiana	Rw2G026810 Rw5G006930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	924
AccB1I	GGYRCC	4 cut(s)	1262, 1472, 1609, 1841
AccII	CGCG	1 cut(s)	2292
AccIII	TCCGGA	1 cut(s)	1483
AclI	AACGTT	1 cut(s)	1517
AclWI	GGATC	7 cut(s)	240, 751, 764, 794, 1475, 1650, 2522
AcoI	YGGCCR	8 cut(s)	70, 1326, 1467, 1867, 1937, 2014, 2421, 2484
AcsI	RAATTY	2 cut(s)	65, 1081
AcuI	CTGAAG	3 cut(s)	882, 1646, 1940
AcyI	GRCGYC	2 cut(s)	389, 2451
AdeI	CACNNNGTG	2 cut(s)	1609, 2294
AfaI	GTAC	9 cut(s)	104, 662, 707, 713, 745, 806, 886, 907, 1806
AfiI	CCNNNNNNNGG	5 cut(s)	47, 417, 1324, 1483, 2060
AflII	CTTAAG	1 cut(s)	1007
AflIII	ACRYGT	4 cut(s)	693, 1539, 1927, 2703
AgeI	ACCGGT	1 cut(s)	740
AhdI	GACNNNNNGTC	1 cut(s)	1931
AjnI	CCWGG	2 cut(s)	41, 1557
AloI	GAACNNNNNNTCC	2 cut(s)	2184, 2216
Alw21I	GWGCWC	4 cut(s)	282, 1790, 1905, 1997
Alw26I	GTCTC	7 cut(s)	344, 372, 396, 442, 1506, 1566, 2672
AlwI	GGATC	7 cut(s)	240, 751, 764, 794, 1475, 1650, 2522
AlwNI	CAGNNNCTG	1 cut(s)	1582
Ama87I	CYCGRG	3 cut(s)	334, 393, 578
Aor13HI	TCCGGA	1 cut(s)	1483
ApoI	RAATTY	2 cut(s)	65, 1081
ArsI	GACNNNNNNTTYG	2 cut(s)	2593, 2625
AseI	ATTAAT	1 cut(s)	2028
AsiGI	ACCGGT	1 cut(s)	740
Asp700I	GAANNNNTTC	2 cut(s)	1058, 1739
AspLEI	GCGC	3 cut(s)	113, 1711, 2530
AspS9I	GGNCC	4 cut(s)	857, 1211, 1822, 2666
AsuC2I	CCSGG	5 cut(s)	289, 335, 336, 1673, 2061
AsuHPI	GGTGA	5 cut(s)	266, 409, 577, 1660, 2309
AvaI	CYCGRG	3 cut(s)	334, 393, 578
AvaII	GGWCC	3 cut(s)	857, 1211, 2666
BaeGI	GKGCMC	1 cut(s)	1673
BaeI	ACNNNNGTAYC	2 cut(s)	86, 119
BalI	TGGCCA	2 cut(s)	1328, 2423
BamHI	GGATCC	1 cut(s)	756
BanI	GGYRCC	4 cut(s)	1262, 1472, 1609, 1841
BanII	GRGCYC	3 cut(s)	42, 282, 424
BarI	GAAGNNNNNNTAC	2 cut(s)	644, 676
BauI	CACGAG	2 cut(s)	313, 2163
BbsI	GAAGAC	3 cut(s)	600, 2247, 2454
Bbv12I	GWGCWC	4 cut(s)	282, 1790, 1905, 1997
BceAI	ACGGC	5 cut(s)	57, 1597, 1787, 1854, 2029
BcgI	CGANNNNNNTGC	2 cut(s)	2234, 2268
BciT130I	CCWGG	2 cut(s)	43, 1559
BclI	TGATCA	2 cut(s)	1387, 2299
BcnI	CCSGG	5 cut(s)	289, 335, 336, 1673, 2061
BcoDI	GTCTC	7 cut(s)	344, 372, 396, 442, 1506, 1566, 2672
BfaI	CTAG	3 cut(s)	275, 441, 494
BfmI	CTRYAG	2 cut(s)	750, 1583
BfoI	RGCGCY	1 cut(s)	114
BfrI	CTTAAG	1 cut(s)	1007
BglII	AGATCT	2 cut(s)	1038, 1548
BlpI	GCTNAGC	2 cut(s)	734, 2550
Bme1390I	CCNGG	7 cut(s)	43, 289, 335, 336, 1559, 1673, 2061
Bme18I	GGWCC	3 cut(s)	857, 1211, 2666
BmeRI	GACNNNNNGTC	1 cut(s)	1931
BmeT110I	CYCGRG	3 cut(s)	334, 393, 578
BmgT120I	GGNCC	4 cut(s)	857, 1211, 1822, 2666
BmrFI	CCNGG	7 cut(s)	43, 289, 335, 336, 1559, 1673, 2061
BmrI	ACTGGG	1 cut(s)	1054
BmsI	GCATC	4 cut(s)	448, 1084, 1477, 1578
BmuI	ACTGGG	1 cut(s)	1054
BpiI	GAAGAC	3 cut(s)	600, 2247, 2454
BplI	GAGNNNNNCTC	2 cut(s)	2669, 2701
BpmI	CTGGAG	3 cut(s)	1544, 1990, 2513
Bpu1102I	GCTNAGC	2 cut(s)	734, 2550
BpuEI	CTTGAG	2 cut(s)	196, 266
BpuMI	CCSGG	5 cut(s)	289, 335, 336, 1673, 2061
Bsa29I	ATCGAT	1 cut(s)	1092
BsaAI	YACGTR	1 cut(s)	1540
BsaBI	GATNNNNATC	2 cut(s)	1392, 2133
BsaHI	GRCGYC	2 cut(s)	389, 2451
BsaI	GGTCTC	3 cut(s)	372, 442, 1566
BsaJI	CCNNGG	9 cut(s)	19, 41, 73, 334, 1558, 1613, 2406, 2424, 2689
BsaWI	WCCGGW	3 cut(s)	562, 740, 1483
Bsc4I	CCNNNNNNNGG	5 cut(s)	47, 417, 1324, 1483, 2060
Bse118I	RCCGGY	4 cut(s)	740, 1465, 1469, 1960
Bse1I	ACTGG	2 cut(s)	1049, 1527
Bse3DI	GCAATG	3 cut(s)	634, 1775, 2054
Bse8I	GATNNNNATC	2 cut(s)	1392, 2133
BseAI	TCCGGA	1 cut(s)	1483
BseBI	CCWGG	2 cut(s)	43, 1559
BseCI	ATCGAT	1 cut(s)	1092
BseDI	CCNNGG	9 cut(s)	19, 41, 73, 334, 1558, 1613, 2406, 2424, 2689
BseGI	GGATG	6 cut(s)	15, 150, 463, 662, 714, 1492
BseJI	GATNNNNATC	2 cut(s)	1392, 2133
BseLI	CCNNNNNNNGG	5 cut(s)	47, 417, 1324, 1483, 2060
BseMI	GCAATG	3 cut(s)	634, 1775, 2054
BseMII	CTCAG	1 cut(s)	2564
BseNI	ACTGG	2 cut(s)	1049, 1527
BseRI	GAGGAG	3 cut(s)	1351, 2040, 2423
BseSI	GKGCMC	1 cut(s)	1673
BseX3I	CGGCCG	1 cut(s)	1467
Bsh1236I	CGCG	1 cut(s)	2292
Bsh1285I	CGRYCG	2 cut(s)	1466, 1470
BshNI	GGYRCC	4 cut(s)	1262, 1472, 1609, 1841
BshTI	ACCGGT	1 cut(s)	740
BshVI	ATCGAT	1 cut(s)	1092
BsiEI	CGRYCG	2 cut(s)	1466, 1470
BsiHKAI	GWGCWC	4 cut(s)	282, 1790, 1905, 1997
BsiHKCI	CYCGRG	3 cut(s)	334, 393, 578
BslFI	GGGAC	4 cut(s)	271, 494, 595, 2679
BslI	CCNNNNNNNGG	5 cut(s)	47, 417, 1324, 1483, 2060
BsmAI	GTCTC	7 cut(s)	344, 372, 396, 442, 1506, 1566, 2672
BsmBI	CGTCTC	2 cut(s)	396, 2672
BsmFI	GGGAC	4 cut(s)	271, 494, 595, 2679
BsmI	GAATGC	2 cut(s)	345, 1229
Bso31I	GGTCTC	3 cut(s)	372, 442, 1566
BsoBI	CYCGRG	3 cut(s)	334, 393, 578
Bsp1286I	GDGCHC	7 cut(s)	42, 282, 424, 1673, 1790, 1905, 1997
Bsp13I	TCCGGA	1 cut(s)	1483
Bsp1407I	TGTACA	1 cut(s)	705
Bsp1720I	GCTNAGC	2 cut(s)	734, 2550
Bsp19I	CCATGG	2 cut(s)	19, 2424
BspCNI	CTCAG	1 cut(s)	2563
BspDI	ATCGAT	1 cut(s)	1092
BspEI	TCCGGA	1 cut(s)	1483
BspFNI	CGCG	1 cut(s)	2292
BspHI	TCATGA	2 cut(s)	633, 2116
BspMAI	CTGCAG	1 cut(s)	1587
BspPI	GGATC	7 cut(s)	240, 751, 764, 794, 1475, 1650, 2522
BspT107I	GGYRCC	4 cut(s)	1262, 1472, 1609, 1841
BspTI	CTTAAG	1 cut(s)	1007
BspTNI	GGTCTC	3 cut(s)	372, 442, 1566
BsrDI	GCAATG	3 cut(s)	634, 1775, 2054
BsrFI	RCCGGY	4 cut(s)	740, 1465, 1469, 1960
BsrGI	TGTACA	1 cut(s)	705
BsrI	ACTGG	2 cut(s)	1049, 1527
BssAI	RCCGGY	4 cut(s)	740, 1465, 1469, 1960
BssECI	CCNNGG	9 cut(s)	19, 41, 73, 334, 1558, 1613, 2406, 2424, 2689
BssNI	GRCGYC	2 cut(s)	389, 2451
BssSI	CACGAG	2 cut(s)	313, 2163
BssT1I	CCWWGG	2 cut(s)	19, 2424
Bst2BI	CACGAG	2 cut(s)	313, 2163
Bst2UI	CCWGG	2 cut(s)	43, 1559
Bst4CI	ACNGT	6 cut(s)	35, 889, 905, 1253, 1535, 1865
Bst6I	CTCTTC	1 cut(s)	1649
BstACI	GRCGYC	2 cut(s)	389, 2451
BstAFI	CTTAAG	1 cut(s)	1007
BstAPI	GCANNNNNTGC	1 cut(s)	1609
BstAUI	TGTACA	1 cut(s)	705
BstBAI	YACGTR	1 cut(s)	1540
BstDEI	CTNAG	5 cut(s)	589, 734, 896, 1411, 2550
BstDSI	CCRYGG	4 cut(s)	19, 73, 1613, 2424
BstEII	GGTNACC	3 cut(s)	254, 291, 565
BstENI	CCTNNNNNAGG	1 cut(s)	415
BstF5I	GGATG	6 cut(s)	15, 150, 463, 662, 714, 1492
BstFNI	CGCG	1 cut(s)	2292
BstH2I	RGCGCY	1 cut(s)	114
BstHHI	GCGC	3 cut(s)	113, 1711, 2530
BstMAI	GTCTC	7 cut(s)	344, 372, 396, 442, 1506, 1566, 2672
BstMCI	CGRYCG	2 cut(s)	1466, 1470
BstNI	CCWGG	2 cut(s)	43, 1559
BstNSI	RCATGY	5 cut(s)	136, 697, 842, 1931, 2707
BstPI	GGTNACC	3 cut(s)	254, 291, 565
BstSCI	CCNGG	7 cut(s)	41, 287, 333, 334, 1557, 1671, 2059
BstSFI	CTRYAG	2 cut(s)	750, 1583
BstSLI	GKGCMC	1 cut(s)	1673
BstUI	CGCG	1 cut(s)	2292
BstV2I	GAAGAC	3 cut(s)	600, 2247, 2454
BstX2I	RGATCY	4 cut(s)	756, 1038, 1480, 1548
BstYI	RGATCY	4 cut(s)	756, 1038, 1480, 1548
BstZI	CGGCCG	1 cut(s)	1467
Bsu15I	ATCGAT	1 cut(s)	1092
BsuTUI	ATCGAT	1 cut(s)	1092
BtgI	CCRYGG	4 cut(s)	19, 73, 1613, 2424
BtsCI	GGATG	6 cut(s)	15, 150, 463, 662, 714, 1492
BtsIMutI	CAGTG	3 cut(s)	40, 297, 977
CaiI	CAGNNNCTG	1 cut(s)	1582
CciI	TCATGA	2 cut(s)	633, 2116
CfoI	GCGC	3 cut(s)	113, 1711, 2530
Cfr10I	RCCGGY	4 cut(s)	740, 1465, 1469, 1960
Cfr13I	GGNCC	4 cut(s)	857, 1211, 1822, 2666
Cfr9I	CCCGGG	1 cut(s)	334
ClaI	ATCGAT	1 cut(s)	1092
CseI	GACGC	3 cut(s)	378, 1977, 2459
Csp6I	GTAC	9 cut(s)	103, 661, 706, 712, 744, 805, 885, 906, 1805
CspAI	ACCGGT	1 cut(s)	740
CspCI	CAANNNNNGTGG	4 cut(s)	1682, 1717, 1819, 1854
CviQI	GTAC	9 cut(s)	103, 661, 706, 712, 744, 805, 885, 906, 1805
DdeI	CTNAG	5 cut(s)	589, 734, 896, 1411, 2550
DraIII	CACNNNGTG	2 cut(s)	1609, 2294
DriI	GACNNNNNGTC	1 cut(s)	1931
EaeI	YGGCCR	8 cut(s)	70, 1326, 1467, 1867, 1937, 2014, 2421, 2484
EagI	CGGCCG	1 cut(s)	1467
Eam1104I	CTCTTC	1 cut(s)	1649
Eam1105I	GACNNNNNGTC	1 cut(s)	1931
EarI	CTCTTC	1 cut(s)	1649
EciI	GGCGGA	4 cut(s)	2444, 2549, 2578, 2701
Ecl136II	GAGCTC	1 cut(s)	280
EclXI	CGGCCG	1 cut(s)	1467
Eco130I	CCWWGG	2 cut(s)	19, 2424
Eco147I	AGGCCT	1 cut(s)	2438
Eco24I	GRGCYC	3 cut(s)	42, 282, 424
Eco31I	GGTCTC	3 cut(s)	372, 442, 1566
Eco47I	GGWCC	3 cut(s)	857, 1211, 2666
Eco52I	CGGCCG	1 cut(s)	1467
Eco53kI	GAGCTC	1 cut(s)	280
Eco57I	CTGAAG	3 cut(s)	882, 1646, 1940
Eco88I	CYCGRG	3 cut(s)	334, 393, 578
Eco91I	GGTNACC	3 cut(s)	254, 291, 565
EcoICRI	GAGCTC	1 cut(s)	280
EcoNI	CCTNNNNNAGG	1 cut(s)	415
EcoO109I	RGGNCCY	3 cut(s)	857, 1211, 2666
EcoO65I	GGTNACC	3 cut(s)	254, 291, 565
EcoRII	CCWGG	2 cut(s)	41, 1557
EcoT14I	CCWWGG	2 cut(s)	19, 2424
EcoT38I	GRGCYC	3 cut(s)	42, 282, 424
ErhI	CCWWGG	2 cut(s)	19, 2424
Esp3I	CGTCTC	2 cut(s)	396, 2672
FalI	AAGNNNNNCTT	4 cut(s)	1724, 1756, 2612, 2644
FaqI	GGGAC	4 cut(s)	271, 494, 595, 2679
FauI	CCCGC	2 cut(s)	1220, 1623
FauNDI	CATATG	1 cut(s)	2184
FbaI	TGATCA	2 cut(s)	1387, 2299
FokI	GGATG	6 cut(s)	2, 137, 470, 669, 701, 1499
FriOI	GRGCYC	3 cut(s)	42, 282, 424
FspBI	CTAG	3 cut(s)	275, 441, 494
GlaI	GCGC	3 cut(s)	112, 1710, 2529
GsuI	CTGGAG	3 cut(s)	1544, 1990, 2513
HaeII	RGCGCY	1 cut(s)	114
HgaI	GACGC	3 cut(s)	378, 1977, 2459
HhaI	GCGC	3 cut(s)	113, 1711, 2530
Hin1I	GRCGYC	2 cut(s)	389, 2451
Hin6I	GCGC	3 cut(s)	111, 1709, 2528
HinP1I	GCGC	3 cut(s)	111, 1709, 2528
HincII	GTYRAC	1 cut(s)	1237
HindII	GTYRAC	1 cut(s)	1237
HindIII	AAGCTT	2 cut(s)	1057, 2267
HinfI	GANTC	8 cut(s)	247, 323, 559, 797, 1526, 1579, 1728, 2312
HpaI	GTTAAC	1 cut(s)	1237
HphI	GGTGA	5 cut(s)	266, 409, 577, 1660, 2309
Hpy166II	GTNNAC	5 cut(s)	908, 1184, 1237, 1249, 1293
Hpy8I	GTNNAC	5 cut(s)	908, 1184, 1237, 1249, 1293
Hpy99I	CGWCG	8 cut(s)	552, 1067, 1280, 1727, 1993, 2015, 2215, 2678
HpyCH4III	ACNGT	6 cut(s)	35, 889, 905, 1253, 1535, 1865
HpyCH4IV	ACGT	2 cut(s)	1517, 1539
HpyF3I	CTNAG	5 cut(s)	589, 734, 896, 1411, 2550
HpySE526I	ACGT	2 cut(s)	1517, 1539
Hsp92I	GRCGYC	2 cut(s)	389, 2451
HspAI	GCGC	3 cut(s)	111, 1709, 2528
KflI	GGGWCCC	1 cut(s)	2666
Kpn2I	TCCGGA	1 cut(s)	1483
KroI	GCCGGC	2 cut(s)	1469, 1960
KroNI	GCCGGC	2 cut(s)	1471, 1962
Ksp22I	TGATCA	2 cut(s)	1387, 2299
KspAI	GTTAAC	1 cut(s)	1237
LmnI	GCTCC	7 cut(s)	1375, 1421, 1675, 1793, 2053, 2536, 2591
LweI	GCATC	4 cut(s)	448, 1084, 1477, 1578
MaeI	CTAG	3 cut(s)	275, 441, 494
MaeII	ACGT	2 cut(s)	1517, 1539
MboII	GAAGA	8 cut(s)	199, 605, 1371, 1608, 1663, 1666, 2252, 2459
MflI	RGATCY	4 cut(s)	756, 1038, 1480, 1548
MhlI	GDGCHC	7 cut(s)	42, 282, 424, 1673, 1790, 1905, 1997
MlsI	TGGCCA	2 cut(s)	1328, 2423
MluCI	AATT	9 cut(s)	65, 182, 525, 535, 768, 851, 947, 1081, 1943
MluNI	TGGCCA	2 cut(s)	1328, 2423
MlyI	GAGTC	2 cut(s)	241, 1535
MmeI	TCCRAC	5 cut(s)	210, 1705, 2193, 2638, 2697
Mox20I	TGGCCA	2 cut(s)	1328, 2423
MroI	TCCGGA	1 cut(s)	1483
MroNI	GCCGGC	2 cut(s)	1469, 1960
MroXI	GAANNNNTTC	2 cut(s)	1058, 1739
MscI	TGGCCA	2 cut(s)	1328, 2423
MseI	TTAA	8 cut(s)	413, 936, 1008, 1152, 1236, 2028, 2196, 2649
MslI	CAYNNNNRTG	3 cut(s)	967, 1395, 2631
Msp20I	TGGCCA	2 cut(s)	1328, 2423
MspCI	CTTAAG	1 cut(s)	1007
MspR9I	CCNGG	7 cut(s)	43, 289, 335, 336, 1559, 1673, 2061
Mva1269I	GAATGC	2 cut(s)	345, 1229
MvaI	CCWGG	2 cut(s)	43, 1559
MvnI	CGCG	1 cut(s)	2292
NaeI	GCCGGC	2 cut(s)	1471, 1962
NciI	CCSGG	5 cut(s)	289, 335, 336, 1673, 2061
NcoI	CCATGG	2 cut(s)	19, 2424
NdeI	CATATG	1 cut(s)	2184
NgoMIV	GCCGGC	2 cut(s)	1469, 1960
NmeAIII	GCCGAG	4 cut(s)	1692, 1704, 2512, 2670
NmuCI	GTSAC	4 cut(s)	88, 254, 565, 973
NspI	RCATGY	5 cut(s)	136, 697, 842, 1931, 2707
PaeI	GCATGC	1 cut(s)	136
PaeR7I	CTCGAG	1 cut(s)	578
PagI	TCATGA	2 cut(s)	633, 2116
PceI	AGGCCT	1 cut(s)	2438
PciI	ACATGT	3 cut(s)	693, 1927, 2703
PcsI	WCGNNNNNNNCGW	1 cut(s)	1719
PctI	GAATGC	2 cut(s)	345, 1229
PdiI	GCCGGC	2 cut(s)	1471, 1962
PdmI	GAANNNNTTC	2 cut(s)	1058, 1739
PfeI	GAWTC	6 cut(s)	323, 559, 797, 1579, 1728, 2312
PinAI	ACCGGT	1 cut(s)	740
PleI	GAGTC	2 cut(s)	241, 1534
PpsI	GAGTC	2 cut(s)	241, 1534
Ppu21I	YACGTR	1 cut(s)	1540
PpuMI	RGGWCCY	3 cut(s)	857, 1211, 2666
PscI	ACATGT	3 cut(s)	693, 1927, 2703
PshBI	ATTAAT	1 cut(s)	2028
PsiI	TTATAA	1 cut(s)	924
Psp124BI	GAGCTC	1 cut(s)	282
Psp1406I	AACGTT	1 cut(s)	1517
Psp5II	RGGWCCY	3 cut(s)	857, 1211, 2666
Psp6I	CCWGG	2 cut(s)	41, 1557
PspEI	GGTNACC	3 cut(s)	254, 291, 565
PspGI	CCWGG	2 cut(s)	41, 1557
PspPI	GGNCC	4 cut(s)	857, 1211, 1822, 2666
PspPPI	RGGWCCY	3 cut(s)	857, 1211, 2666
PstI	CTGCAG	1 cut(s)	1587
PstNI	CAGNNNCTG	1 cut(s)	1582
PsuI	RGATCY	4 cut(s)	756, 1038, 1480, 1548
RsaI	GTAC	9 cut(s)	104, 662, 707, 713, 745, 806, 886, 907, 1806
RsaNI	GTAC	9 cut(s)	103, 661, 706, 712, 744, 805, 885, 906, 1805
RseI	CAYNNNNRTG	3 cut(s)	967, 1395, 2631
SacI	GAGCTC	1 cut(s)	282
SaqAI	TTAA	8 cut(s)	413, 936, 1008, 1152, 1236, 2028, 2196, 2649
Sau96I	GGNCC	4 cut(s)	857, 1211, 1822, 2666
SchI	GAGTC	2 cut(s)	241, 1535
ScrFI	CCNGG	7 cut(s)	43, 289, 335, 336, 1559, 1673, 2061
SduI	GDGCHC	7 cut(s)	42, 282, 424, 1673, 1790, 1905, 1997
SfaNI	GCATC	4 cut(s)	448, 1084, 1477, 1578
SfcI	CTRYAG	2 cut(s)	750, 1583
Sfr274I	CTCGAG	1 cut(s)	578
SinI	GGWCC	3 cut(s)	857, 1211, 2666
SlaI	CTCGAG	1 cut(s)	578
SmaI	CCCGGG	1 cut(s)	336
SmiMI	CAYNNNNRTG	3 cut(s)	967, 1395, 2631
SmlI	CTYRAG	4 cut(s)	211, 281, 578, 1007
SmoI	CTYRAG	4 cut(s)	211, 281, 578, 1007
SphI	GCATGC	1 cut(s)	136
Sse9I	AATT	9 cut(s)	65, 182, 525, 535, 768, 851, 947, 1081, 1943
SseBI	AGGCCT	1 cut(s)	2438
SspMI	CTAG	3 cut(s)	275, 441, 494
SstI	GAGCTC	1 cut(s)	282
StuI	AGGCCT	1 cut(s)	2438
StyD4I	CCNGG	7 cut(s)	41, 287, 333, 334, 1557, 1671, 2059
StyI	CCWWGG	2 cut(s)	19, 2424
TaaI	ACNGT	6 cut(s)	35, 889, 905, 1253, 1535, 1865
TaiI	ACGT	2 cut(s)	1520, 1542
TaqI	TCGA	7 cut(s)	579, 671, 849, 1014, 1092, 1991, 2233
TasI	AATT	9 cut(s)	65, 182, 525, 535, 768, 851, 947, 1081, 1943
TatI	WGTACW	3 cut(s)	705, 711, 905
TauI	GCSGC	6 cut(s)	828, 961, 1967, 1970, 2283, 2473
TfiI	GAWTC	6 cut(s)	323, 559, 797, 1579, 1728, 2312
Tru1I	TTAA	8 cut(s)	413, 936, 1008, 1152, 1236, 2028, 2196, 2649
Tru9I	TTAA	8 cut(s)	413, 936, 1008, 1152, 1236, 2028, 2196, 2649
TscAI	CASTG	3 cut(s)	40, 304, 984
TseFI	GTSAC	4 cut(s)	88, 254, 565, 973
Tsp45I	GTSAC	4 cut(s)	88, 254, 565, 973
TspDTI	ATGAA	6 cut(s)	228, 259, 701, 1221, 1885, 2354
TspMI	CCCGGG	1 cut(s)	334
TspRI	CASTG	3 cut(s)	40, 304, 984
Vha464I	CTTAAG	1 cut(s)	1007
VpaK11BI	GGWCC	3 cut(s)	857, 1211, 2666
VspI	ATTAAT	1 cut(s)	2028
XagI	CCTNNNNNAGG	1 cut(s)	415
XapI	RAATTY	2 cut(s)	65, 1081
XceI	RCATGY	5 cut(s)	136, 697, 842, 1931, 2707
XhoI	CTCGAG	1 cut(s)	578
XmaI	CCCGGG	1 cut(s)	334
XmnI	GAANNNNTTC	2 cut(s)	1058, 1739
XspI	CTAG	3 cut(s)	275, 441, 494

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.