Rorug05G0051400

TLC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001172
Physical Location & Seq
Forward (+)
4517890 .. 4518909
1020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0051400.1

Sequence Viewer

Length: 933 bp
ATGCTCCCAAACCCTAGCCGCCTGTGCTCGGTGCTCCAAACATGGAGATCACTACAGGTCTCCATTCTAAGTGGAGAAACCCGCTCCACACCCCGCACTCCAATCTCAGCCCTCCAACGCTGGTTTTTCCCAGCCACCACCCAACAAAATCAAGGTTTTAAAACCTTCAATGTCTCTGTTTCTTGCCACAATGAGGTCGATCCGGAGATCGGAAGTTTGCGACCCAAACCTAGCCCTAAGCCTTTCATCACCATGTTTGCTTCGCGCCGGAGTGATATGGTTGATGAGAGTAAAAGTGATCGGTGGTCTGATTTGGGGAAACCCAAGCCTAGATGGGTGCGAGTATGGAATTTCTGGATTCGTCCATGTCGGGCTCGACTCAAATCGGACCTGCTTTTGGTTGGCGAAGCAACGCATGATCGCCTCTTCCTCGACCCGATCCGGACGGCGACGCTGACCTGCGGTGCACATCAGGGTGTTTCCAGTGCCCCAAGCAAATCGATGGACGTCGTTGCACTCCAACCTGCGGGTGTGACTGTGCTCCGATGTGCAGAAGCATTAGCAAGGTACGCTGCTGCGACAAGTCACGCCGAGGCAACAATCTGGGTTACGAAGGAGGCAAGCAGCTCTGCGGCGGGAGGTTTGGCCGTGAGCGGCAGAGAGGCAGAAGTAGGGGTGCCCAGATCAGTTTTGGGACCCAAATGGTTTCGGAGGCCCAAATTTGGTGGGAGGCCTAAATTGTTTTGGGTGTGCGATGCTACTTGGAATTGGTCTTGGCCTCATGTATGGACTAAGCTAACGGACTCAGGCTCTCTAGGATTTCGTATTTGGGATCCAGGAGGCTTAATCCAGCAAATTACACTTCTATTCTCTATGTTTGCAATGAGTCTATATACTATGTTTATGAGTCATAGTACACTAGGCTTGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAM_LAG1_CLN8

Protein Analysis

310

Amino Acids

34.58

Weight (kDa)

10.08

Isoelectric Point (pI)

46.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 510
Acc36I ACCTGC 3 cut(s) 399, 467, 532
AccB1I GGYRCC 1 cut(s) 676
AccBSI CCGCTC 2 cut(s) 84, 654
AccII CGCG 1 cut(s) 265
AccIII TCCGGA 2 cut(s) 202, 441
AciI CCGC 8 cut(s) 19, 82, 94, 462, 527, 632, 635, 654
AclWI GGATC 4 cut(s) 194, 433, 827, 840
AcoI YGGCCR 1 cut(s) 645
AcsI RAATTY 2 cut(s) 349, 719
AcyI GRCGYC 1 cut(s) 507
AfaI GTAC 2 cut(s) 569, 916
AfiI CCNNNNNNNGG 6 cut(s) 28, 193, 209, 397, 526, 722
AgsI TTSAA 1 cut(s) 169
AjnI CCWGG 1 cut(s) 835
AluBI AGCT 2 cut(s) 627, 796
AluI AGCT 2 cut(s) 627, 796
Alw21I GWGCWC 4 cut(s) 29, 36, 469, 543
Alw26I GTCTC 2 cut(s) 64, 178
Alw44I GTGCAC 1 cut(s) 465
AlwI GGATC 4 cut(s) 194, 433, 827, 840
Aor13HI TCCGGA 2 cut(s) 202, 441
AoxI GGCC 4 cut(s) 645, 713, 731, 776
ApaLI GTGCAC 1 cut(s) 465
ApeKI GCWGC 3 cut(s) 572, 575, 624
ApoI RAATTY 2 cut(s) 349, 719
AspLEI GCGC 1 cut(s) 267
AspS9I GGNCC 3 cut(s) 388, 695, 714
AsuHPI GGTGA 1 cut(s) 241
AvaII GGWCC 2 cut(s) 388, 695
BaeGI GKGCMC 3 cut(s) 469, 490, 681
BamHI GGATCC 1 cut(s) 832
BanI GGYRCC 1 cut(s) 676
BanII GRGCYC 1 cut(s) 376
Bbv12I GWGCWC 4 cut(s) 29, 36, 469, 543
BbvI GCAGC 3 cut(s) 559, 562, 636
BccI CCATC 2 cut(s) 327, 496
BceAI ACGGC 2 cut(s) 462, 632
BciT130I CCWGG 1 cut(s) 837
BcoDI GTCTC 2 cut(s) 64, 178
BfaI CTAG 5 cut(s) 15, 231, 330, 815, 920
BfmI CTRYAG 1 cut(s) 53
BfuAI ACCTGC 3 cut(s) 399, 467, 532
BisI GCNGC 6 cut(s) 19, 573, 576, 625, 633, 655
BlsI GCNGC 6 cut(s) 20, 574, 577, 626, 634, 656
Bme1390I CCNGG 1 cut(s) 837
Bme18I GGWCC 2 cut(s) 388, 695
BmgT120I GGNCC 3 cut(s) 388, 695, 714
BmiI GGNNCC 4 cut(s) 678, 696, 697, 834
BmrFI CCNGG 1 cut(s) 837
BmsI GCATC 1 cut(s) 745
Bpu10I CCTNAGC 1 cut(s) 237
Bsa29I ATCGAT 1 cut(s) 500
BsaHI GRCGYC 1 cut(s) 507
BsaI GGTCTC 1 cut(s) 64
BsaJI CCNNGG 1 cut(s) 591
BsaWI WCCGGW 2 cut(s) 202, 441
Bsc4I CCNNNNNNNGG 6 cut(s) 28, 193, 209, 397, 526, 722
Bse1I ACTGG 1 cut(s) 483
Bse3DI GCAATG 1 cut(s) 888
BseAI TCCGGA 2 cut(s) 202, 441
BseBI CCWGG 1 cut(s) 837
BseCI ATCGAT 1 cut(s) 500
BseDI CCNNGG 1 cut(s) 591
BseLI CCNNNNNNNGG 6 cut(s) 28, 193, 209, 397, 526, 722
BseMI GCAATG 1 cut(s) 888
BseMII CTCAG 2 cut(s) 120, 819
BseNI ACTGG 1 cut(s) 483
BseSI GKGCMC 3 cut(s) 469, 490, 681
BseXI GCAGC 3 cut(s) 559, 562, 636
BseYI CCCAGC 1 cut(s) 130
BsgI GTGCAG 1 cut(s) 570
Bsh1236I CGCG 1 cut(s) 265
BshFI GGCC 4 cut(s) 647, 715, 733, 778
BshNI GGYRCC 1 cut(s) 676
BshVI ATCGAT 1 cut(s) 500
BsiHKAI GWGCWC 4 cut(s) 29, 36, 469, 543
BsiSI CCGG 3 cut(s) 203, 268, 442
BslFI GGGAC 1 cut(s) 708
BslI CCNNNNNNNGG 6 cut(s) 28, 193, 209, 397, 526, 722
BsmAI GTCTC 2 cut(s) 64, 178
BsmFI GGGAC 1 cut(s) 708
BsnI GGCC 4 cut(s) 647, 715, 733, 778
Bso31I GGTCTC 1 cut(s) 64
Bsp1286I GDGCHC 7 cut(s) 29, 36, 376, 469, 490, 543, 681
Bsp13I TCCGGA 2 cut(s) 202, 441
Bsp143I GATC 8 cut(s) 47, 199, 207, 298, 418, 438, 683, 832
BspACI CCGC 8 cut(s) 19, 82, 94, 462, 527, 632, 635, 654
BspANI GGCC 4 cut(s) 647, 715, 733, 778
BspCNI CTCAG 2 cut(s) 119, 818
BspDI ATCGAT 1 cut(s) 500
BspEI TCCGGA 2 cut(s) 202, 441
BspFNI CGCG 1 cut(s) 265
BspLI GGNNCC 4 cut(s) 678, 696, 697, 834
BspMI ACCTGC 3 cut(s) 399, 467, 532
BspPI GGATC 4 cut(s) 194, 433, 827, 840
BspT107I GGYRCC 1 cut(s) 676
BspTNI GGTCTC 1 cut(s) 64
BsrBI CCGCTC 2 cut(s) 84, 654
BsrDI GCAATG 1 cut(s) 888
BsrI ACTGG 1 cut(s) 483
BssECI CCNNGG 1 cut(s) 591
BssMI GATC 8 cut(s) 47, 199, 207, 298, 418, 438, 683, 832
BssNI GRCGYC 1 cut(s) 507
Bst2UI CCWGG 1 cut(s) 837
Bst4CI ACNGT 1 cut(s) 538
Bst6I CTCTTC 1 cut(s) 431
BstACI GRCGYC 1 cut(s) 507
BstC8I GCNNGC 1 cut(s) 622
BstDEI CTNAG 5 cut(s) 68, 106, 237, 792, 805
BstFNI CGCG 1 cut(s) 265
BstHHI GCGC 1 cut(s) 267
BstKTI GATC 8 cut(s) 50, 202, 210, 301, 421, 441, 686, 835
BstMAI GTCTC 2 cut(s) 64, 178
BstMBI GATC 8 cut(s) 47, 199, 207, 298, 418, 438, 683, 832
BstMWI GCNNNNNNNGC 2 cut(s) 24, 569
BstNI CCWGG 1 cut(s) 837
BstSCI CCNGG 1 cut(s) 835
BstSFI CTRYAG 1 cut(s) 53
BstSLI GKGCMC 3 cut(s) 469, 490, 681
BstUI CGCG 1 cut(s) 265
BstV1I GCAGC 3 cut(s) 559, 562, 636
BstX2I RGATCY 1 cut(s) 832
BstYI RGATCY 1 cut(s) 832
Bsu15I ATCGAT 1 cut(s) 500
BsuRI GGCC 4 cut(s) 647, 715, 733, 778
BsuTUI ATCGAT 1 cut(s) 500
BtgZI GCGATG 1 cut(s) 768
BtsIMutI CAGTG 1 cut(s) 490
BveI ACCTGC 3 cut(s) 399, 467, 532
Cac8I GCNNGC 1 cut(s) 622
CfoI GCGC 1 cut(s) 267
Cfr13I GGNCC 3 cut(s) 388, 695, 714
ClaI ATCGAT 1 cut(s) 500
CseI GACGC 1 cut(s) 460
Csp6I GTAC 2 cut(s) 568, 915
CspCI CAANNNNNGTGG 2 cut(s) 706, 741
CviAII CATG 5 cut(s) 42, 253, 366, 416, 782
CviQI GTAC 2 cut(s) 568, 915
DdeI CTNAG 5 cut(s) 68, 106, 237, 792, 805
DpnI GATC 8 cut(s) 49, 201, 209, 300, 420, 440, 685, 834
DpnII GATC 8 cut(s) 47, 199, 207, 298, 418, 438, 683, 832
DraI TTTAAA 1 cut(s) 160
EaeI YGGCCR 1 cut(s) 645
Eam1104I CTCTTC 1 cut(s) 431
EarI CTCTTC 1 cut(s) 431
Eco147I AGGCCT 1 cut(s) 733
Eco24I GRGCYC 1 cut(s) 376
Eco31I GGTCTC 1 cut(s) 64
Eco47I GGWCC 2 cut(s) 388, 695
EcoO109I RGGNCCY 1 cut(s) 695
EcoRII CCWGG 1 cut(s) 835
EcoT38I GRGCYC 1 cut(s) 376
FaeI CATG 5 cut(s) 45, 256, 369, 419, 785
FaqI GGGAC 1 cut(s) 708
FatI CATG 5 cut(s) 41, 252, 365, 415, 781
FauI CCCGC 4 cut(s) 89, 101, 520, 628
Fnu4HI GCNGC 6 cut(s) 19, 573, 576, 625, 633, 655
FriOI GRGCYC 1 cut(s) 376
Fsp4HI GCNGC 6 cut(s) 19, 573, 576, 625, 633, 655
FspBI CTAG 5 cut(s) 15, 231, 330, 815, 920
GlaI GCGC 1 cut(s) 266
GluI GCNGC 6 cut(s) 19, 573, 576, 625, 633, 655
GsaI CCCAGC 1 cut(s) 134
HaeIII GGCC 4 cut(s) 647, 715, 733, 778
HapII CCGG 3 cut(s) 203, 268, 442
HgaI GACGC 1 cut(s) 460
HhaI GCGC 1 cut(s) 267
Hin1I GRCGYC 1 cut(s) 507
Hin1II CATG 5 cut(s) 45, 256, 369, 419, 785
Hin6I GCGC 1 cut(s) 265
HinP1I GCGC 1 cut(s) 265
HinfI GANTC 5 cut(s) 358, 378, 803, 886, 907
HpaII CCGG 3 cut(s) 203, 268, 442
HphI GGTGA 1 cut(s) 241
Hpy166II GTNNAC 2 cut(s) 467, 917
Hpy188I TCNGA 6 cut(s) 212, 310, 388, 545, 711, 932
Hpy188III TCNNGA 3 cut(s) 203, 355, 442
Hpy8I GTNNAC 2 cut(s) 467, 917
Hpy99I CGWCG 2 cut(s) 454, 512
HpyAV CCTTC 2 cut(s) 175, 607
HpyCH4III ACNGT 1 cut(s) 538
HpyCH4IV ACGT 1 cut(s) 507
HpyCH4V TGCA 4 cut(s) 467, 515, 551, 881
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 569
HpyF3I CTNAG 5 cut(s) 68, 106, 237, 792, 805
HpySE526I ACGT 1 cut(s) 507
Hsp92I GRCGYC 1 cut(s) 507
Hsp92II CATG 5 cut(s) 45, 256, 369, 419, 785
HspAI GCGC 1 cut(s) 265
KflI GGGWCCC 1 cut(s) 695
Kpn2I TCCGGA 2 cut(s) 202, 441
Kzo9I GATC 8 cut(s) 47, 199, 207, 298, 418, 438, 683, 832
LmnI GCTCC 4 cut(s) 9, 39, 89, 546
Lsp1109I GCAGC 3 cut(s) 559, 562, 636
LweI GCATC 1 cut(s) 745
MaeI CTAG 5 cut(s) 15, 231, 330, 815, 920
MaeII ACGT 1 cut(s) 507
MaeIII GTNAC 3 cut(s) 532, 584, 607
MalI GATC 8 cut(s) 49, 201, 209, 300, 420, 440, 685, 834
MbiI CCGCTC 2 cut(s) 84, 654
MboI GATC 8 cut(s) 47, 199, 207, 298, 418, 438, 683, 832
MboII GAAGA 1 cut(s) 418
MflI RGATCY 1 cut(s) 832
MhlI GDGCHC 7 cut(s) 29, 36, 376, 469, 490, 543, 681
MluCI AATT 5 cut(s) 349, 719, 737, 766, 855
MlyI GAGTC 4 cut(s) 372, 797, 895, 916
MmeI TCCRAC 2 cut(s) 139, 544
MroI TCCGGA 2 cut(s) 202, 441
MseI TTAA 2 cut(s) 159, 845
MslI CAYNNNNRTG 2 cut(s) 251, 474
MspI CCGG 3 cut(s) 203, 268, 442
MspR9I CCNGG 1 cut(s) 837
MvaI CCWGG 1 cut(s) 837
MvnI CGCG 1 cut(s) 265
MwoI GCNNNNNNNGC 2 cut(s) 24, 569
NdeII GATC 8 cut(s) 47, 199, 207, 298, 418, 438, 683, 832
NlaIII CATG 5 cut(s) 45, 256, 369, 419, 785
NlaIV GGNNCC 4 cut(s) 678, 696, 697, 834
NmeAIII GCCGAG 1 cut(s) 616
NmuCI GTSAC 2 cut(s) 532, 584
PceI AGGCCT 1 cut(s) 733
PfeI GAWTC 1 cut(s) 358
PfoI TCCNGGA 1 cut(s) 835
PkrI GCNGC 6 cut(s) 20, 574, 577, 626, 634, 656
PleI GAGTC 4 cut(s) 372, 797, 894, 915
PpsI GAGTC 4 cut(s) 372, 797, 894, 915
PpuMI RGGWCCY 1 cut(s) 695
Psp5II RGGWCCY 1 cut(s) 695
Psp6I CCWGG 1 cut(s) 835
PspFI CCCAGC 1 cut(s) 130
PspGI CCWGG 1 cut(s) 835
PspN4I GGNNCC 4 cut(s) 678, 696, 697, 834
PspPI GGNCC 3 cut(s) 388, 695, 714
PspPPI RGGWCCY 1 cut(s) 695
PsuI RGATCY 1 cut(s) 832
RsaI GTAC 2 cut(s) 569, 916
RsaNI GTAC 2 cut(s) 568, 915
RseI CAYNNNNRTG 2 cut(s) 251, 474
SaqAI TTAA 2 cut(s) 159, 845
SatI GCNGC 6 cut(s) 19, 573, 576, 625, 633, 655
Sau3AI GATC 8 cut(s) 47, 199, 207, 298, 418, 438, 683, 832
Sau96I GGNCC 3 cut(s) 388, 695, 714
SchI GAGTC 4 cut(s) 372, 797, 895, 916
ScrFI CCNGG 1 cut(s) 837
SduI GDGCHC 7 cut(s) 29, 36, 376, 469, 490, 543, 681
SfaNI GCATC 1 cut(s) 745
SfcI CTRYAG 1 cut(s) 53
SinI GGWCC 2 cut(s) 388, 695
SmiMI CAYNNNNRTG 2 cut(s) 251, 474
Sse9I AATT 5 cut(s) 349, 719, 737, 766, 855
SseBI AGGCCT 1 cut(s) 733
SsiI CCGC 8 cut(s) 19, 82, 94, 462, 527, 632, 635, 654
SspMI CTAG 5 cut(s) 15, 231, 330, 815, 920
StuI AGGCCT 1 cut(s) 733
StyD4I CCNGG 1 cut(s) 835
TaaI ACNGT 1 cut(s) 538
TaiI ACGT 1 cut(s) 510
TaqI TCGA 4 cut(s) 198, 376, 432, 500
TasI AATT 5 cut(s) 349, 719, 737, 766, 855
TatI WGTACW 1 cut(s) 914
TauI GCSGC 3 cut(s) 21, 635, 657
TfiI GAWTC 1 cut(s) 358
Tru1I TTAA 2 cut(s) 159, 845
Tru9I TTAA 2 cut(s) 159, 845
TscAI CASTG 1 cut(s) 490
TseFI GTSAC 2 cut(s) 532, 584
TseI GCWGC 3 cut(s) 572, 575, 624
Tsp45I GTSAC 2 cut(s) 532, 584
TspDTI ATGAA 1 cut(s) 235
TspGWI ACGGA 1 cut(s) 815
TspRI CASTG 1 cut(s) 490
VneI GTGCAC 1 cut(s) 465
VpaK11BI GGWCC 2 cut(s) 388, 695
XapI RAATTY 2 cut(s) 349, 719
XcmI CCANNNNNNNNNTGG 1 cut(s) 688
XspI CTAG 5 cut(s) 15, 231, 330, 815, 920
ZraI GACGTC 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.