Rw2G040660

Transmembrane protein 56-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001172
Physical Location & Seq
Reverse (-)
66289223 .. 66291823
2601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G040660.1

Sequence Viewer

Length: 699 bp
ATGGCAATCAAATCCTATGAGATGGTGGCCGAGCAATACCTGCTCTCTGACCCTTTTGTTCCTTACACATCTATGCTTTTGGGGATTTGCGCCTGCAAATTGGTTTACCATGTTAACCATTTGCTCAGTGCTGCTTACTTCAAGGGATATTCTGGCCTTTCGAGGATGAAACGTATTGAGTGGAACAATAGGGCTATGTCAACTCTTCATGCCATTTTTATCACAGCTATGTCATTGTACTTGGTGTTTTGGTCGAATCTCTATTCCGACTATTCTGACAACCAACTCAAGCCATTTGTTACTTTGCAGAGCTCCCCATTGTCTACATTTGTATTGGGGGTTTCAGTTGGGTACTTCCTATCTGACCTTGGCATGATCTTTTGGTTTTACCCTGCTCTAGGTGGATTGGAATATGTGATTCATCATCTTCTTTCTATAGCATCTGTAGCATATGCAATGTTGACAGGGGAAGGACAACTGTATACCTACATGGTTCTTATCTCTGAGACAACCACCCCCGGTATCAATTTGAGATGGTATCTTGACACTGCTGGAATGAAGAGGACTAAAACTTATATGATAAATGGGGTTGTAATATTCATTGCATGGCTGGTTGCCAGAATCCTTCTGTTCATGTTTCTGTTCTACCATCTCTACCTGCATTATGAACAGGTGATTTGTACGCCTACCAGTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAM_LAG1_CLN8

Protein Analysis

232

Amino Acids

26.59

Weight (kDa)

7.72

Isoelectric Point (pI)

42.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 60 - 224 1.2e-27 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 48, 666
AccI GTMKAC 2 cut(s) 323, 482
AcoI YGGCCR 1 cut(s) 27
AfaI GTAC 4 cut(s) 239, 353, 682, 694
AfiI CCNNNNNNNGG 1 cut(s) 398
AgsI TTSAA 1 cut(s) 142
AluBI AGCT 2 cut(s) 227, 312
AluI AGCT 2 cut(s) 227, 312
Alw21I GWGCWC 1 cut(s) 314
Alw26I GTCTC 1 cut(s) 500
AoxI GGCC 2 cut(s) 27, 154
ApeKI GCWGC 1 cut(s) 131
AspLEI GCGC 1 cut(s) 92
AsuC2I CCSGG 1 cut(s) 519
AsuHPI GGTGA 1 cut(s) 685
BanII GRGCYC 1 cut(s) 314
Bbv12I GWGCWC 1 cut(s) 314
BbvI GCAGC 1 cut(s) 118
BccI CCATC 3 cut(s) 16, 528, 657
BcnI CCSGG 1 cut(s) 519
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 1 cut(s) 398
BfmI CTRYAG 2 cut(s) 435, 444
BfuAI ACCTGC 2 cut(s) 48, 666
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
BmcAI AGTACT 1 cut(s) 694
Bme1390I CCNGG 1 cut(s) 519
BmrFI CCNGG 1 cut(s) 519
BmsI GCATC 1 cut(s) 449
BpuEI CTTGAG 1 cut(s) 272
BpuMI CCSGG 1 cut(s) 519
BsaJI CCNNGG 2 cut(s) 367, 517
Bsc4I CCNNNNNNNGG 1 cut(s) 398
Bse1I ACTGG 1 cut(s) 690
Bse3DI GCAATG 2 cut(s) 462, 600
BseDI CCNNGG 2 cut(s) 367, 517
BseGI GGATG 1 cut(s) 171
BseLI CCNNNNNNNGG 1 cut(s) 398
BseMI GCAATG 2 cut(s) 462, 600
BseMII CTCAG 2 cut(s) 139, 495
BseNI ACTGG 1 cut(s) 690
BseXI GCAGC 1 cut(s) 118
BshFI GGCC 2 cut(s) 29, 156
BsiHKAI GWGCWC 1 cut(s) 314
BsiSI CCGG 1 cut(s) 519
BslI CCNNNNNNNGG 1 cut(s) 398
BsmAI GTCTC 1 cut(s) 500
BsnI GGCC 2 cut(s) 29, 156
Bsp1286I GDGCHC 1 cut(s) 314
Bsp143I GATC 1 cut(s) 375
BspANI GGCC 2 cut(s) 29, 156
BspCNI CTCAG 2 cut(s) 138, 496
BspMI ACCTGC 2 cut(s) 48, 666
BsrDI GCAATG 2 cut(s) 462, 600
BsrI ACTGG 1 cut(s) 690
BssECI CCNNGG 2 cut(s) 367, 517
BssMI GATC 1 cut(s) 375
BssNAI GTATAC 1 cut(s) 483
BssT1I CCWWGG 1 cut(s) 367
Bst1107I GTATAC 1 cut(s) 483
Bst4CI ACNGT 1 cut(s) 480
Bst6I CTCTTC 2 cut(s) 210, 554
BstAPI GCANNNNNTGC 1 cut(s) 40
BstC8I GCNNGC 1 cut(s) 94
BstDEI CTNAG 2 cut(s) 125, 504
BstENI CCTNNNNNAGG 1 cut(s) 396
BstF5I GGATG 1 cut(s) 171
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 1 cut(s) 378
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 1 cut(s) 375
BstMWI GCNNNNNNNGC 2 cut(s) 40, 446
BstSCI CCNGG 1 cut(s) 517
BstSFI CTRYAG 2 cut(s) 435, 444
BstV1I GCAGC 1 cut(s) 118
BstZ17I GTATAC 1 cut(s) 483
BsuRI GGCC 2 cut(s) 29, 156
BtsCI GGATG 1 cut(s) 171
BtsI GCAGTG 1 cut(s) 546
BtsIMutI CAGTG 2 cut(s) 133, 546
BveI ACCTGC 2 cut(s) 48, 666
Cac8I GCNNGC 1 cut(s) 94
CfoI GCGC 1 cut(s) 92
Csp6I GTAC 4 cut(s) 238, 352, 681, 693
CviAII CATG 6 cut(s) 110, 209, 373, 490, 606, 634
CviJI RGCY 7 cut(s) 29, 156, 194, 227, 292, 312, 610
CviKI_1 RGCY 7 cut(s) 29, 156, 194, 227, 292, 312, 610
CviQI GTAC 4 cut(s) 238, 352, 681, 693
DdeI CTNAG 2 cut(s) 125, 504
DpnI GATC 1 cut(s) 377
DpnII GATC 1 cut(s) 375
EaeI YGGCCR 1 cut(s) 27
Eam1104I CTCTTC 2 cut(s) 210, 554
EarI CTCTTC 2 cut(s) 210, 554
Ecl136II GAGCTC 1 cut(s) 312
Eco130I CCWWGG 1 cut(s) 367
Eco24I GRGCYC 1 cut(s) 314
Eco53kI GAGCTC 1 cut(s) 312
EcoICRI GAGCTC 1 cut(s) 312
EcoNI CCTNNNNNAGG 1 cut(s) 396
EcoT14I CCWWGG 1 cut(s) 367
EcoT38I GRGCYC 1 cut(s) 314
ErhI CCWWGG 1 cut(s) 367
FaeI CATG 6 cut(s) 113, 212, 376, 493, 609, 637
FatI CATG 6 cut(s) 109, 208, 372, 489, 605, 633
FauNDI CATATG 1 cut(s) 451
FblI GTMKAC 2 cut(s) 323, 482
Fnu4HI GCNGC 1 cut(s) 132
FokI GGATG 1 cut(s) 178
FriOI GRGCYC 1 cut(s) 314
Fsp4HI GCNGC 1 cut(s) 132
FspBI CTAG 1 cut(s) 398
GlaI GCGC 1 cut(s) 91
GluI GCNGC 1 cut(s) 132
HaeIII GGCC 2 cut(s) 29, 156
HapII CCGG 1 cut(s) 519
HhaI GCGC 1 cut(s) 92
Hin1II CATG 6 cut(s) 113, 212, 376, 493, 609, 637
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HincII GTYRAC 3 cut(s) 115, 201, 462
HindII GTYRAC 3 cut(s) 115, 201, 462
HinfI GANTC 3 cut(s) 256, 418, 621
HpaI GTTAAC 1 cut(s) 115
HpaII CCGG 1 cut(s) 519
HphI GGTGA 1 cut(s) 685
Hpy166II GTNNAC 6 cut(s) 106, 115, 201, 324, 462, 483
Hpy188I TCNGA 5 cut(s) 49, 268, 277, 364, 505
Hpy188III TCNNGA 1 cut(s) 542
Hpy8I GTNNAC 6 cut(s) 106, 115, 201, 324, 462, 483
HpyAV CCTTC 2 cut(s) 464, 635
HpyCH4III ACNGT 1 cut(s) 480
HpyCH4IV ACGT 1 cut(s) 172
HpyCH4V TGCA 5 cut(s) 96, 307, 455, 605, 661
HpyF10VI GCNNNNNNNGC 2 cut(s) 40, 446
HpyF3I CTNAG 2 cut(s) 125, 504
HpySE526I ACGT 1 cut(s) 172
Hsp92II CATG 6 cut(s) 113, 212, 376, 493, 609, 637
HspAI GCGC 1 cut(s) 90
KspAI GTTAAC 1 cut(s) 115
Kzo9I GATC 1 cut(s) 375
LmnI GCTCC 1 cut(s) 317
Lsp1109I GCAGC 1 cut(s) 118
LweI GCATC 1 cut(s) 449
MaeI CTAG 1 cut(s) 398
MaeII ACGT 1 cut(s) 172
MaeIII GTNAC 1 cut(s) 298
MalI GATC 1 cut(s) 377
MboI GATC 1 cut(s) 375
MboII GAAGA 3 cut(s) 197, 419, 571
MhlI GDGCHC 1 cut(s) 314
MluCI AATT 2 cut(s) 98, 526
MmeI TCCRAC 1 cut(s) 291
MnlI CCTC 2 cut(s) 156, 555
MseI TTAA 2 cut(s) 114, 697
MslI CAYNNNNRTG 2 cut(s) 71, 227
MspI CCGG 1 cut(s) 519
MspR9I CCNGG 1 cut(s) 519
MwoI GCNNNNNNNGC 2 cut(s) 40, 446
NciI CCSGG 1 cut(s) 519
NdeI CATATG 1 cut(s) 451
NdeII GATC 1 cut(s) 375
NlaIII CATG 6 cut(s) 113, 212, 376, 493, 609, 637
NmeAIII GCCGAG 1 cut(s) 55
PfeI GAWTC 3 cut(s) 256, 418, 621
PkrI GCNGC 1 cut(s) 133
Psp124BI GAGCTC 1 cut(s) 314
RsaI GTAC 4 cut(s) 239, 353, 682, 694
RsaNI GTAC 4 cut(s) 238, 352, 681, 693
RseI CAYNNNNRTG 2 cut(s) 71, 227
SacI GAGCTC 1 cut(s) 314
SaqAI TTAA 2 cut(s) 114, 697
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 1 cut(s) 375
ScaI AGTACT 1 cut(s) 694
ScrFI CCNGG 1 cut(s) 519
SduI GDGCHC 1 cut(s) 314
SetI ASST 9 cut(s) 42, 175, 229, 314, 369, 403, 488, 660, 675
SfaNI GCATC 1 cut(s) 449
SfcI CTRYAG 2 cut(s) 435, 444
SmiMI CAYNNNNRTG 2 cut(s) 71, 227
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
Sse9I AATT 2 cut(s) 98, 526
SspI AATATT 1 cut(s) 597
SspMI CTAG 1 cut(s) 398
SstI GAGCTC 1 cut(s) 314
StyD4I CCNGG 1 cut(s) 517
StyI CCWWGG 1 cut(s) 367
TaaI ACNGT 1 cut(s) 480
TaiI ACGT 1 cut(s) 175
TaqI TCGA 2 cut(s) 161, 254
TasI AATT 2 cut(s) 98, 526
TatI WGTACW 2 cut(s) 237, 692
TfiI GAWTC 3 cut(s) 256, 418, 621
Tru1I TTAA 2 cut(s) 114, 697
Tru9I TTAA 2 cut(s) 114, 697
TscAI CASTG 2 cut(s) 133, 553
TseI GCWGC 1 cut(s) 131
TspDTI ATGAA 7 cut(s) 182, 197, 410, 572, 589, 622, 681
TspRI CASTG 2 cut(s) 133, 553
XagI CCTNNNNNAGG 1 cut(s) 396
XmiI GTMKAC 2 cut(s) 323, 482
XspI CTAG 1 cut(s) 398
ZrmI AGTACT 1 cut(s) 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.