Rorug06G0061400

Endoplasmic reticulum metallopeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011628
Physical Location & Seq
Forward (+)
8216852 .. 8217996
1145 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0061400.1

Sequence Viewer

Length: 705 bp
ATGGCTCAGGCTGTGCTGCACGCCTGCTCTCTGCTTCTTTGGTCGGAACCCGTTGCTTCACTCCCTCTACAGGTATTTGTTCCTCTACGTAAAGCGTTCAAGTTAACATCAGTGCAGAAGAAGAAGCCATCATTCCGAAAGCATAAGGCCTTGGTTGCTTTGAAAATTTACCAGAAGATCTTCAGGCCTCAGGGTTTCGAAAGTGAGCTTTTATACCCCTATTGTTTGAAGATTGTGGACTTGCTGGGAATGCAAGCATTGGTGAGGGGGCTAAAACTTCCTAATCAAATAGTAGCTTACTTGATGAACAAGCAAATAAGTGATGAAACTGCAGATCTAGAATGGGAGAAACTAGTACGAGAGTTGTCAAACAAAGGCATGTTTAAGAATTGTGTTGTTGTGTGTGACATGCCGAGCATGAAAGGGACAAAGACGGAGGTGGCCAGCATTTCAATGGGGTTATTGATTTCAGAACTGAGTGCAAATCCGTGGAAAGGAAAAGTATTCTCATCGGCAGGAAGGATGTTCAAGATGCACAATGTTGAAGGAGACAGTCTCAGACCAAAATGTGAGTTCATGAGGCAGCACATAGAGTGTGTAGAGATGGTGGATATCCACAATATTTACAACCAAGTTCTGCAGATTGCCACAGAAGAAAAGCTAAGCAACATGGAGATGCCCGAGAAGATTTTAGTGCTCACTTAG

Protein Analysis

234

Amino Acids

26.65

Weight (kDa)

9.15

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7788 PF25043 130 - 233 3e-12 Domain of unknown function (DUF7788)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 441
AcsI RAATTY 1 cut(s) 165
AcuI CTGAAG 1 cut(s) 166
AfaI GTAC 1 cut(s) 357
AfiI CCNNNNNNNGG 2 cut(s) 70, 494
AgsI TTSAA 6 cut(s) 100, 163, 229, 453, 529, 545
AhlI ACTAGT 1 cut(s) 352
AluBI AGCT 3 cut(s) 208, 296, 661
AluI AGCT 3 cut(s) 208, 296, 661
Alw21I GWGCWC 1 cut(s) 699
Alw26I GTCTC 2 cut(s) 543, 560
Ama87I CYCGRG 1 cut(s) 680
AoxI GGCC 3 cut(s) 147, 185, 441
ApeKI GCWGC 2 cut(s) 16, 583
ApoI RAATTY 1 cut(s) 165
Asp700I GAANNNNTTC 1 cut(s) 179
AsuHPI GGTGA 1 cut(s) 274
AsuII TTCGAA 1 cut(s) 198
AvaI CYCGRG 1 cut(s) 680
AxyI CCTNAGG 1 cut(s) 189
BalI TGGCCA 1 cut(s) 443
Bbv12I GWGCWC 1 cut(s) 699
BbvI GCAGC 2 cut(s) 3, 595
BccI CCATC 2 cut(s) 136, 598
BcoDI GTCTC 2 cut(s) 543, 560
BcuI ACTAGT 1 cut(s) 352
BfaI CTAG 2 cut(s) 338, 353
BfmI CTRYAG 3 cut(s) 68, 330, 638
BglII AGATCT 2 cut(s) 177, 334
BisI GCNGC 2 cut(s) 17, 584
BlpI GCTNAGC 1 cut(s) 662
BlsI GCNGC 2 cut(s) 18, 585
BmeT110I CYCGRG 1 cut(s) 680
BmiI GGNNCC 1 cut(s) 48
BmsI GCATC 2 cut(s) 522, 666
BplI GAGNNNNNCTC 2 cut(s) 540, 572
Bpu10I CCTNAGC 1 cut(s) 6
Bpu1102I GCTNAGC 1 cut(s) 662
Bpu14I TTCGAA 1 cut(s) 198
BsaAI YACGTR 1 cut(s) 89
BsaJI CCNNGG 2 cut(s) 150, 488
Bsc4I CCNNNNNNNGG 2 cut(s) 70, 494
Bse21I CCTNAGG 1 cut(s) 189
BseDI CCNNGG 2 cut(s) 150, 488
BseGI GGATG 1 cut(s) 528
BseLI CCNNNNNNNGG 2 cut(s) 70, 494
BseMII CTCAG 4 cut(s) 20, 203, 467, 571
BseXI GCAGC 2 cut(s) 3, 595
BseYI CCCAGC 1 cut(s) 244
BsgI GTGCAG 1 cut(s) 134
BshFI GGCC 3 cut(s) 149, 187, 443
BsiHKAI GWGCWC 1 cut(s) 699
BsiHKCI CYCGRG 1 cut(s) 680
BslFI GGGAC 1 cut(s) 439
BslI CCNNNNNNNGG 2 cut(s) 70, 494
BsmAI GTCTC 2 cut(s) 543, 560
BsmFI GGGAC 1 cut(s) 439
BsmI GAATGC 1 cut(s) 255
BsnI GGCC 3 cut(s) 149, 187, 443
BsoBI CYCGRG 1 cut(s) 680
Bsp119I TTCGAA 1 cut(s) 198
Bsp1286I GDGCHC 1 cut(s) 699
Bsp143I GATC 2 cut(s) 177, 334
Bsp1720I GCTNAGC 1 cut(s) 662
BspANI GGCC 3 cut(s) 149, 187, 443
BspCNI CTCAG 4 cut(s) 19, 202, 468, 570
BspHI TCATGA 1 cut(s) 576
BspLI GGNNCC 1 cut(s) 48
BspMAI CTGCAG 2 cut(s) 334, 642
BspT104I TTCGAA 1 cut(s) 198
BssECI CCNNGG 2 cut(s) 150, 488
BssMI GATC 2 cut(s) 177, 334
BssT1I CCWWGG 1 cut(s) 150
Bst4CI ACNGT 1 cut(s) 554
BstBAI YACGTR 1 cut(s) 89
BstBI TTCGAA 1 cut(s) 198
BstC8I GCNNGC 4 cut(s) 21, 25, 255, 445
BstDEI CTNAG 6 cut(s) 6, 189, 476, 557, 662, 702
BstDSI CCRYGG 1 cut(s) 488
BstF5I GGATG 1 cut(s) 528
BstKTI GATC 2 cut(s) 180, 337
BstMAI GTCTC 2 cut(s) 543, 560
BstMBI GATC 2 cut(s) 177, 334
BstMWI GCNNNNNNNGC 2 cut(s) 155, 250
BstNSI RCATGY 2 cut(s) 382, 412
BstSFI CTRYAG 3 cut(s) 68, 330, 638
BstSNI TACGTA 1 cut(s) 89
BstV1I GCAGC 2 cut(s) 3, 595
BstX2I RGATCY 2 cut(s) 177, 334
BstYI RGATCY 2 cut(s) 177, 334
Bsu36I CCTNAGG 1 cut(s) 189
BsuRI GGCC 3 cut(s) 149, 187, 443
BtgI CCRYGG 1 cut(s) 488
BtsCI GGATG 1 cut(s) 528
BtsIMutI CAGTG 1 cut(s) 117
Cac8I GCNNGC 4 cut(s) 21, 25, 255, 445
CciI TCATGA 1 cut(s) 576
Csp6I GTAC 1 cut(s) 356
CviAII CATG 5 cut(s) 379, 409, 418, 577, 670
CviQI GTAC 1 cut(s) 356
DdeI CTNAG 6 cut(s) 6, 189, 476, 557, 662, 702
DpnI GATC 2 cut(s) 179, 336
DpnII GATC 2 cut(s) 177, 334
EaeI YGGCCR 1 cut(s) 441
Eco105I TACGTA 1 cut(s) 89
Eco130I CCWWGG 1 cut(s) 150
Eco147I AGGCCT 2 cut(s) 149, 187
Eco32I GATATC 1 cut(s) 613
Eco57I CTGAAG 1 cut(s) 166
Eco81I CCTNAGG 1 cut(s) 189
Eco88I CYCGRG 1 cut(s) 680
EcoRV GATATC 1 cut(s) 613
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 5 cut(s) 382, 412, 421, 580, 673
FaiI YATR 8 cut(s) 144, 214, 380, 410, 419, 578, 590, 671
FaqI GGGAC 1 cut(s) 439
FatI CATG 5 cut(s) 378, 408, 417, 576, 669
Fnu4HI GCNGC 2 cut(s) 17, 584
FokI GGATG 1 cut(s) 535
Fsp4HI GCNGC 2 cut(s) 17, 584
FspBI CTAG 2 cut(s) 338, 353
GluI GCNGC 2 cut(s) 17, 584
GsaI CCCAGC 1 cut(s) 248
HaeIII GGCC 3 cut(s) 149, 187, 443
Hin1II CATG 5 cut(s) 382, 412, 421, 580, 673
HincII GTYRAC 1 cut(s) 105
HindII GTYRAC 1 cut(s) 105
HpaI GTTAAC 1 cut(s) 105
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 2 cut(s) 105, 238
Hpy188I TCNGA 4 cut(s) 46, 137, 472, 560
Hpy188III TCNNGA 3 cut(s) 338, 529, 577
Hpy8I GTNNAC 2 cut(s) 105, 238
HpyAV CCTTC 2 cut(s) 513, 539
HpyCH4III ACNGT 1 cut(s) 554
HpyCH4IV ACGT 1 cut(s) 88
HpyCH4V TGCA 7 cut(s) 19, 115, 253, 332, 482, 535, 640
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 250
HpyF3I CTNAG 6 cut(s) 6, 189, 476, 557, 662, 702
HpySE526I ACGT 1 cut(s) 88
Hsp92II CATG 5 cut(s) 382, 412, 421, 580, 673
KspAI GTTAAC 1 cut(s) 105
Kzo9I GATC 2 cut(s) 177, 334
LpnPI CCDG 8 cut(s) 37, 56, 169, 176, 185, 230, 457, 501
Lsp1109I GCAGC 2 cut(s) 3, 595
LweI GCATC 2 cut(s) 522, 666
MaeI CTAG 2 cut(s) 338, 353
MaeII ACGT 1 cut(s) 88
MaeIII GTNAC 1 cut(s) 404
MalI GATC 2 cut(s) 179, 336
MboI GATC 2 cut(s) 177, 334
MboII GAAGA 7 cut(s) 130, 133, 172, 187, 241, 665, 697
MflI RGATCY 2 cut(s) 177, 334
MhlI GDGCHC 1 cut(s) 699
MlsI TGGCCA 1 cut(s) 443
MluCI AATT 2 cut(s) 165, 388
MluNI TGGCCA 1 cut(s) 443
MmeI TCCRAC 1 cut(s) 24
MnlI CCTC 6 cut(s) 75, 93, 198, 258, 430, 573
Mox20I TGGCCA 1 cut(s) 443
MroXI GAANNNNTTC 1 cut(s) 179
MscI TGGCCA 1 cut(s) 443
MseI TTAA 2 cut(s) 104, 384
MslI CAYNNNNRTG 2 cut(s) 452, 674
Msp20I TGGCCA 1 cut(s) 443
Mva1269I GAATGC 1 cut(s) 255
MwoI GCNNNNNNNGC 2 cut(s) 155, 250
NdeII GATC 2 cut(s) 177, 334
NlaIII CATG 5 cut(s) 382, 412, 421, 580, 673
NlaIV GGNNCC 1 cut(s) 48
NmeAIII GCCGAG 1 cut(s) 438
NmuCI GTSAC 1 cut(s) 404
NspI RCATGY 2 cut(s) 382, 412
NspV TTCGAA 1 cut(s) 198
PagI TCATGA 1 cut(s) 576
PceI AGGCCT 2 cut(s) 149, 187
PctI GAATGC 1 cut(s) 255
PdmI GAANNNNTTC 1 cut(s) 179
PkrI GCNGC 2 cut(s) 18, 585
Ppu21I YACGTR 1 cut(s) 89
PspFI CCCAGC 1 cut(s) 244
PspN4I GGNNCC 1 cut(s) 48
PstI CTGCAG 2 cut(s) 334, 642
PsuI RGATCY 2 cut(s) 177, 334
RsaI GTAC 1 cut(s) 357
RsaNI GTAC 1 cut(s) 356
RseI CAYNNNNRTG 2 cut(s) 452, 674
SaqAI TTAA 2 cut(s) 104, 384
SatI GCNGC 2 cut(s) 17, 584
Sau3AI GATC 2 cut(s) 177, 334
SduI GDGCHC 1 cut(s) 699
SetI ASST 6 cut(s) 75, 91, 210, 298, 441, 663
SfaNI GCATC 2 cut(s) 522, 666
SfcI CTRYAG 3 cut(s) 68, 330, 638
SfuI TTCGAA 1 cut(s) 198
SmiMI CAYNNNNRTG 2 cut(s) 452, 674
SnaBI TACGTA 1 cut(s) 89
SpeI ACTAGT 1 cut(s) 352
Sse9I AATT 2 cut(s) 165, 388
SseBI AGGCCT 2 cut(s) 149, 187
SspI AATATT 1 cut(s) 622
SspMI CTAG 2 cut(s) 338, 353
StuI AGGCCT 2 cut(s) 149, 187
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 1 cut(s) 554
TaiI ACGT 1 cut(s) 91
TaqI TCGA 1 cut(s) 198
TasI AATT 2 cut(s) 165, 388
Tru1I TTAA 2 cut(s) 104, 384
Tru9I TTAA 2 cut(s) 104, 384
TscAI CASTG 1 cut(s) 117
TseFI GTSAC 1 cut(s) 404
TseI GCWGC 2 cut(s) 16, 583
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 4 cut(s) 320, 339, 434, 565
TspGWI ACGGA 2 cut(s) 449, 477
TspRI CASTG 1 cut(s) 117
XapI RAATTY 1 cut(s) 165
XbaI TCTAGA 1 cut(s) 337
XceI RCATGY 2 cut(s) 382, 412
XcmI CCANNNNNNNNNTGG 1 cut(s) 451
XmnI GAANNNNTTC 1 cut(s) 179
XspI CTAG 2 cut(s) 338, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.