Rorug06G0359300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007114
Physical Location & Seq
Forward (+)
50810384 .. 50811638
1255 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0359300.1

Sequence Viewer

Length: 504 bp
ATGGCGCAAACCCTGGCAATGCCTCTGGCGCCGTCTCTCTCCGTAGTCTGCAACGGCCGAAACCCTAATCCGTTCTCTACCACTCTCTCACTCCCAGTCAGAACCCCAAACAAGGTAGGTGGTTTAAGCATCAAATGTGTGCGTGTTGGCGGAGTTGAAATCCCAAACAACAAGAGGGTGGAGTTTTCTCTTCAGTACGTTCATGGAATTGGGCGTACCCGAGCACGTACAATCCTGAACGACCTGAAAATGGAGAACAAGGTCACCAAAGACTTGTCTGAAGAAGAGCTTACTATAATTCGTGAGGAGGTCTCCAAGTACATGATTGAAGGAGACTTGAGGCGGTTCAATGCTTTGAACATTAGGAGATTGAAGGAGATTCAGTGCTACAGGGGGATAAGGCATATTCAGGGTCTGCCTTGTAGAGGGCAGAGGACTAAGAACAACACCAGGACTTTGAAGGGTAAGAGGGTCACTGTTGCTGGAAAGAAGAAAGCTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAN_4

Protein Analysis

167

Amino Acids

18.84

Weight (kDa)

10.7

Isoelectric Point (pI)

50.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S13 PF00416 48 - 152 1.5e-30 Ribosomal protein S13/S18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 28
AciI CCGC 2 cut(s) 150, 343
AcoI YGGCCR 1 cut(s) 55
AcuI CTGAAG 2 cut(s) 176, 300
AcyI GRCGYC 1 cut(s) 29
AfaI GTAC 4 cut(s) 197, 217, 229, 320
AfiI CCNNNNNNNGG 3 cut(s) 112, 250, 425
AgsI TTSAA 6 cut(s) 158, 329, 349, 358, 373, 460
AjnI CCWGG 2 cut(s) 12, 449
AluBI AGCT 2 cut(s) 289, 497
AluI AGCT 2 cut(s) 289, 497
Alw21I GWGCWC 1 cut(s) 226
Alw26I GTCTC 3 cut(s) 39, 316, 327
AlwNI CAGNNNCTG 1 cut(s) 415
Ama87I CYCGRG 1 cut(s) 219
AoxI GGCC 1 cut(s) 55
AspLEI GCGC 2 cut(s) 7, 31
AsuHPI GGTGA 1 cut(s) 256
AvaI CYCGRG 1 cut(s) 219
BanI GGYRCC 1 cut(s) 28
Bbv12I GWGCWC 1 cut(s) 226
BceAI ACGGC 2 cut(s) 16, 70
BciT130I CCWGG 2 cut(s) 14, 451
BcoDI GTCTC 3 cut(s) 39, 316, 327
BfmI CTRYAG 1 cut(s) 388
BfoI RGCGCY 1 cut(s) 32
Bme1390I CCNGG 2 cut(s) 14, 451
BmeT110I CYCGRG 1 cut(s) 219
BmiI GGNNCC 1 cut(s) 30
BmrFI CCNGG 2 cut(s) 14, 451
BmrI ACTGGG 1 cut(s) 89
BmsI GCATC 1 cut(s) 138
BmuI ACTGGG 1 cut(s) 89
BplI GAGNNNNNCTC 2 cut(s) 296, 328
BpuEI CTTGAG 1 cut(s) 358
BsaAI YACGTR 1 cut(s) 227
BsaHI GRCGYC 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 316
BsaJI CCNNGG 1 cut(s) 12
BsaXI ACNNNNNCTCC 2 cut(s) 368, 398
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 250, 425
Bse1I ACTGG 1 cut(s) 95
Bse3DI GCAATG 1 cut(s) 24
BseBI CCWGG 2 cut(s) 14, 451
BseDI CCNNGG 1 cut(s) 12
BseLI CCNNNNNNNGG 3 cut(s) 112, 250, 425
BseMI GCAATG 1 cut(s) 24
BseNI ACTGG 1 cut(s) 95
BseRI GAGGAG 1 cut(s) 320
BseX3I CGGCCG 1 cut(s) 55
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 1 cut(s) 57
BshNI GGYRCC 1 cut(s) 28
BsiEI CGRYCG 1 cut(s) 58
BsiHKAI GWGCWC 1 cut(s) 226
BsiHKCI CYCGRG 1 cut(s) 219
BslI CCNNNNNNNGG 3 cut(s) 112, 250, 425
BsmAI GTCTC 3 cut(s) 39, 316, 327
BsmBI CGTCTC 1 cut(s) 39
BsnI GGCC 1 cut(s) 57
Bso31I GGTCTC 1 cut(s) 316
BsoBI CYCGRG 1 cut(s) 219
Bsp1286I GDGCHC 1 cut(s) 226
BspACI CCGC 2 cut(s) 150, 343
BspANI GGCC 1 cut(s) 57
BspLI GGNNCC 1 cut(s) 30
BspQI GCTCTTC 1 cut(s) 279
BspT107I GGYRCC 1 cut(s) 28
BspTNI GGTCTC 1 cut(s) 316
BsrDI GCAATG 1 cut(s) 24
BsrI ACTGG 1 cut(s) 95
BssECI CCNNGG 1 cut(s) 12
BssNI GRCGYC 1 cut(s) 29
Bst2UI CCWGG 2 cut(s) 14, 451
Bst4CI ACNGT 1 cut(s) 478
Bst6I CTCTTC 2 cut(s) 195, 279
BstACI GRCGYC 1 cut(s) 29
BstBAI YACGTR 1 cut(s) 227
BstDEI CTNAG 1 cut(s) 438
BstEII GGTNACC 1 cut(s) 262
BstENI CCTNNNNNAGG 1 cut(s) 423
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 2 cut(s) 7, 31
BstMAI GTCTC 3 cut(s) 39, 316, 327
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 1 cut(s) 28
BstNI CCWGG 2 cut(s) 14, 451
BstPI GGTNACC 1 cut(s) 262
BstSCI CCNGG 2 cut(s) 12, 449
BstSFI CTRYAG 1 cut(s) 388
BstZI CGGCCG 1 cut(s) 55
BsuRI GGCC 1 cut(s) 57
BtsIMutI CAGTG 2 cut(s) 389, 474
CaiI CAGNNNCTG 1 cut(s) 415
CfoI GCGC 2 cut(s) 7, 31
Csp6I GTAC 4 cut(s) 196, 216, 228, 319
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CviAII CATG 2 cut(s) 203, 322
CviJI RGCY 3 cut(s) 57, 289, 497
CviKI_1 RGCY 3 cut(s) 57, 289, 497
CviQI GTAC 4 cut(s) 196, 216, 228, 319
DdeI CTNAG 1 cut(s) 438
DinI GGCGCC 1 cut(s) 30
EaeI YGGCCR 1 cut(s) 55
EagI CGGCCG 1 cut(s) 55
Eam1104I CTCTTC 2 cut(s) 195, 279
EarI CTCTTC 2 cut(s) 195, 279
EciI GGCGGA 1 cut(s) 165
EclXI CGGCCG 1 cut(s) 55
Eco31I GGTCTC 1 cut(s) 316
Eco52I CGGCCG 1 cut(s) 55
Eco57I CTGAAG 2 cut(s) 176, 300
Eco88I CYCGRG 1 cut(s) 219
Eco91I GGTNACC 1 cut(s) 262
EcoNI CCTNNNNNAGG 1 cut(s) 423
EcoO65I GGTNACC 1 cut(s) 262
EcoRII CCWGG 2 cut(s) 12, 449
EgeI GGCGCC 1 cut(s) 30
EheI GGCGCC 1 cut(s) 30
Esp3I CGTCTC 1 cut(s) 39
FaeI CATG 2 cut(s) 206, 325
FaiI YATR 4 cut(s) 204, 296, 323, 405
FalI AAGNNNNNCTT 2 cut(s) 273, 305
FatI CATG 2 cut(s) 202, 321
GlaI GCGC 2 cut(s) 6, 30
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 1 cut(s) 57
HhaI GCGC 2 cut(s) 7, 31
Hin1I GRCGYC 1 cut(s) 29
Hin1II CATG 2 cut(s) 206, 325
Hin6I GCGC 2 cut(s) 5, 29
HinP1I GCGC 2 cut(s) 5, 29
HinfI GANTC 1 cut(s) 379
HphI GGTGA 1 cut(s) 256
Hpy188I TCNGA 2 cut(s) 101, 280
Hpy188III TCNNGA 2 cut(s) 235, 302
HpyAV CCTTC 3 cut(s) 323, 367, 454
HpyCH4III ACNGT 1 cut(s) 478
HpyCH4IV ACGT 2 cut(s) 198, 226
HpyCH4V TGCA 1 cut(s) 51
HpyF10VI GCNNNNNNNGC 1 cut(s) 28
HpyF3I CTNAG 1 cut(s) 438
HpySE526I ACGT 2 cut(s) 198, 226
Hsp92I GRCGYC 1 cut(s) 29
Hsp92II CATG 2 cut(s) 206, 325
HspAI GCGC 2 cut(s) 5, 29
KasI GGCGCC 1 cut(s) 28
LguI GCTCTTC 1 cut(s) 279
LweI GCATC 1 cut(s) 138
MaeII ACGT 2 cut(s) 198, 226
MaeIII GTNAC 2 cut(s) 262, 472
MboII GAAGA 4 cut(s) 182, 293, 296, 502
MhlI GDGCHC 1 cut(s) 226
MluCI AATT 2 cut(s) 207, 297
Mly113I GGCGCC 1 cut(s) 29
MnlI CCTC 8 cut(s) 33, 168, 298, 301, 333, 419, 426, 462
MseI TTAA 2 cut(s) 125, 502
MspR9I CCNGG 2 cut(s) 14, 451
MvaI CCWGG 2 cut(s) 14, 451
MwoI GCNNNNNNNGC 1 cut(s) 28
NarI GGCGCC 1 cut(s) 29
NlaIII CATG 2 cut(s) 206, 325
NlaIV GGNNCC 1 cut(s) 30
NmuCI GTSAC 2 cut(s) 262, 472
PciSI GCTCTTC 1 cut(s) 279
PfeI GAWTC 1 cut(s) 379
PluTI GGCGCC 1 cut(s) 32
Ppu21I YACGTR 1 cut(s) 227
Psp6I CCWGG 2 cut(s) 12, 449
PspEI GGTNACC 1 cut(s) 262
PspGI CCWGG 2 cut(s) 12, 449
PspN4I GGNNCC 1 cut(s) 30
PstNI CAGNNNCTG 1 cut(s) 415
RsaI GTAC 4 cut(s) 197, 217, 229, 320
RsaNI GTAC 4 cut(s) 196, 216, 228, 319
SapI GCTCTTC 1 cut(s) 279
SaqAI TTAA 2 cut(s) 125, 502
ScrFI CCNGG 2 cut(s) 14, 451
SduI GDGCHC 1 cut(s) 226
SetI ASST 9 cut(s) 117, 121, 201, 229, 246, 264, 291, 312, 499
SfaNI GCATC 1 cut(s) 138
SfcI CTRYAG 1 cut(s) 388
SfoI GGCGCC 1 cut(s) 30
SmlI CTYRAG 1 cut(s) 337
SmoI CTYRAG 1 cut(s) 337
Sse9I AATT 2 cut(s) 207, 297
SsiI CCGC 2 cut(s) 150, 343
SspDI GGCGCC 1 cut(s) 28
StyD4I CCNGG 2 cut(s) 12, 449
TaaI ACNGT 1 cut(s) 478
TaiI ACGT 2 cut(s) 201, 229
TasI AATT 2 cut(s) 207, 297
TatI WGTACW 1 cut(s) 318
TfiI GAWTC 1 cut(s) 379
Tru1I TTAA 2 cut(s) 125, 502
Tru9I TTAA 2 cut(s) 125, 502
TscAI CASTG 2 cut(s) 389, 481
TseFI GTSAC 2 cut(s) 262, 472
Tsp45I GTSAC 2 cut(s) 262, 472
TspDTI ATGAA 1 cut(s) 191
TspGWI ACGGA 2 cut(s) 31, 60
TspRI CASTG 2 cut(s) 389, 481
XagI CCTNNNNNAGG 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.