Rh1AG468100

B3 domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0004389
Physical Location & Seq
Forward (+)
68181813 .. 68184248
2436 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG468100.1

Sequence Viewer

Length: 1566 bp
ATGGCCTTTCCTCCATCGGAAAATTCACTCCGGCTGTCGGATTCTGTGGCGAATTTCTTCAAAGTTATACTTCCCCCCACCATTGAGAATCAGAAACTGGATATCCCACTAAAGTCTGCACTAAAATTTGGGGATGAACTGTCTAATGTTGCTACACTAATTGTTCCAGCTGGTCGTGTTTGGCAAGTGGATTTGGAAAGAGCTCACAAGAGGATTTGGTTTGTCGGCCGTTGGCAAAAATTCGCAGAATATTACTCCTTAACTGATGGTCAGTTCTTAGTCTTTAGTTATCAAGGAAATTCGACATTCCATGTTCGCATTTTTTATTTCAGTTGTTGTGAGATCGAGTACCCTTGTGTTGGTCAAAGTATCAAACATGAAGAGGATGTTGAAGATGATGTTTGTGGAACTCTCCCTTCTTCCAGCAAAGGCAAAGGCAAATGTGTAGATCAACATGTTAAGAGGAAGAAGAAAAGTGTTGCTATTGCCAAGAGATTTGAAGAGGATGTTGAAGATGATGTTTATGGAACTCGCCCTTCTTCCAGCAAAGGCAAAGGCAAAGGCAAATGTGTAGATCAACATGTTAAGAGGAAGAGGGAAAGCGTTGCTATTGCCAAGAGATTTAAGCGAGCCAAGTTGAAGGAACAGAGAATGCCTAGCTCACAGGAATTTCCCACAAGAAAGAAGAAATATAAGGAAATATTTGTTAAGAACATCGCTGCTTACACTTATCCTAATTGCAAAATTACGATTGCCAAAGAACAGCAGAAAGTGATCCATGCTACCAAAATGTTCAACTTGGAAAATCCTTTTTTTGCAGTTATCTTGCAGCAATGCAATCTTGACAACTACTATATGTATGTTCCAACTGCCTTTGGAAGGAGATATATGAGAGGGTGTCCAGAATATATCAAGTTTGAGGATTCTAATGAAAAGCAGTGGGATATCCGATGCTTTTTCCCATCAAGTACAAAGGTGATGAGGAGAATAGGCAAGGGATGTGGTGAATTTTTCAGAGACAATAATTTCAAGAATGGAGATGTCTGTCTCTTTGAGCTGATCAAAAGGAAGGATGTTGTGTTAAAATTTTCGGTATTGCGCAACGCTGAATATGCAGCCAGGGTCGGTGGGAAAAAGAAAAATCCAAAGATTCTTGATAGTGGAACCTTGTACTTCTCTAGGAAGATTAAAGACCTGGTAATATCCAGAGAAACCAAGAAGGCAGTGAAGGATGCGCGAGCTTTGAAGATTGAAAATCCTGCTTTCATGGTTATCTTGCGAGCGCACAACATGCACAACTCTTATGTGCGTGTGCCTGCTGAGTTTGTGAAGGATCATCTGCTTGGATGTGCTAACTTGATCAACCTTAAGGTCTCTAGTGTCAAGAGGGGCGTGCGCTGCGCTTATGAGCCTGCACGCCACAGTATGGAGATACGCAGAGGATTTGGTGAATTTGCTGAGGATAATAATTTGGAGGAAGGAGATGTTTGTGTGTTTGAGCTGATCAAGAGTGAGGCTGATGCGCTCAAAGTTTCAATATTCCATGCCAGTGAATATGCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

521

Amino Acids

59.93

Weight (kDa)

9.47

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 19 - 108 5.7e-12 B3 DNA binding domain
B3 PF02362 271 - 368 1.9e-12 B3 DNA binding domain
B3 PF02362 424 - 516 1.9e-11 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1100
AccB7I CCANNNNNTGG 1 cut(s) 1426
AccII CGCG 1 cut(s) 1237
AclWI GGATC 2 cut(s) 769, 1341
AcoI YGGCCR 1 cut(s) 226
AcsI RAATTY 9 cut(s) 22, 52, 125, 239, 298, 668, 1007, 1085, 1451
AfaI GTAC 3 cut(s) 350, 970, 1172
AfiI CCNNNNNNNGG 4 cut(s) 37, 359, 879, 1426
AflII CTTAAG 1 cut(s) 1367
AflIII ACRYGT 2 cut(s) 454, 580
AjnI CCWGG 2 cut(s) 1118, 1194
AluBI AGCT 6 cut(s) 170, 203, 660, 1057, 1241, 1501
AluI AGCT 6 cut(s) 170, 203, 660, 1057, 1241, 1501
Alw21I GWGCWC 1 cut(s) 205
Alw26I GTCTC 3 cut(s) 1011, 1052, 1378
AlwI GGATC 2 cut(s) 769, 1341
AlwNI CAGNNNCTG 1 cut(s) 97
AoxI GGCC 2 cut(s) 3, 226
ApeKI GCWGC 4 cut(s) 719, 829, 1115, 1398
ApoI RAATTY 9 cut(s) 22, 52, 125, 239, 298, 668, 1007, 1085, 1451
Asp700I GAANNNNTTC 1 cut(s) 56
AspLEI GCGC 6 cut(s) 1101, 1237, 1285, 1398, 1403, 1525
AsuHPI GGTGA 3 cut(s) 988, 1016, 1460
BanII GRGCYC 1 cut(s) 205
Bbv12I GWGCWC 1 cut(s) 205
BbvCI CCTCAGC 1 cut(s) 1458
BbvI GCAGC 4 cut(s) 706, 841, 1127, 1385
BccI CCATC 3 cut(s) 22, 260, 970
BceAI ACGGC 1 cut(s) 213
BciT130I CCWGG 2 cut(s) 1120, 1196
BclI TGATCA 3 cut(s) 1059, 1359, 1503
BcoDI GTCTC 3 cut(s) 1011, 1052, 1378
BfaI CTAG 3 cut(s) 657, 1179, 1377
BfrI CTTAAG 1 cut(s) 1367
BisI GCNGC 4 cut(s) 720, 830, 1116, 1399
BlsI GCNGC 4 cut(s) 721, 831, 1117, 1400
Bme1390I CCNGG 2 cut(s) 1120, 1196
BmiI GGNNCC 1 cut(s) 1165
BmrFI CCNGG 2 cut(s) 1120, 1196
BmsI GCATC 3 cut(s) 941, 1222, 1510
Bpu10I CCTNAGC 1 cut(s) 1458
BsaI GGTCTC 1 cut(s) 1378
BsaJI CCNNGG 1 cut(s) 1119
Bsc4I CCNNNNNNNGG 4 cut(s) 37, 359, 879, 1426
Bse1I ACTGG 2 cut(s) 102, 1548
Bse3DI GCAATG 1 cut(s) 839
BseBI CCWGG 2 cut(s) 1120, 1196
BseDI CCNNGG 1 cut(s) 1119
BseGI GGATG 7 cut(s) 139, 391, 511, 1004, 1078, 1237, 1352
BseLI CCNNNNNNNGG 4 cut(s) 37, 359, 879, 1426
BseMI GCAATG 1 cut(s) 839
BseMII CTCAG 2 cut(s) 1311, 1449
BseNI ACTGG 2 cut(s) 102, 1548
BseRI GAGGAG 1 cut(s) 997
BseX3I CGGCCG 1 cut(s) 226
BseXI GCAGC 4 cut(s) 706, 841, 1127, 1385
BsgI GTGCAG 2 cut(s) 102, 1398
Bsh1236I CGCG 1 cut(s) 1237
Bsh1285I CGRYCG 1 cut(s) 229
BshFI GGCC 2 cut(s) 5, 228
BsiEI CGRYCG 1 cut(s) 229
BsiHKAI GWGCWC 1 cut(s) 205
BsiSI CCGG 1 cut(s) 31
BslI CCNNNNNNNGG 4 cut(s) 37, 359, 879, 1426
BsmAI GTCTC 3 cut(s) 1011, 1052, 1378
BsmI GAATGC 1 cut(s) 657
BsnI GGCC 2 cut(s) 5, 228
Bso31I GGTCTC 1 cut(s) 1378
Bsp1286I GDGCHC 1 cut(s) 205
Bsp143I GATC 8 cut(s) 342, 448, 574, 774, 1059, 1333, 1359, 1503
BspANI GGCC 2 cut(s) 5, 228
BspCNI CTCAG 2 cut(s) 1312, 1450
BspFNI CGCG 1 cut(s) 1237
BspLI GGNNCC 1 cut(s) 1165
BspPI GGATC 2 cut(s) 769, 1341
BspTI CTTAAG 1 cut(s) 1367
BspTNI GGTCTC 1 cut(s) 1378
BsrDI GCAATG 1 cut(s) 839
BsrI ACTGG 2 cut(s) 102, 1548
BssECI CCNNGG 1 cut(s) 1119
BssMI GATC 8 cut(s) 342, 448, 574, 774, 1059, 1333, 1359, 1503
Bst2UI CCWGG 2 cut(s) 1120, 1196
Bst4CI ACNGT 2 cut(s) 141, 1424
Bst6I CTCTTC 3 cut(s) 375, 495, 587
BstAFI CTTAAG 1 cut(s) 1367
BstAPI GCANNNNNTGC 1 cut(s) 1291
BstC8I GCNNGC 7 cut(s) 630, 1239, 1281, 1317, 1394, 1413, 1417
BstDEI CTNAG 3 cut(s) 277, 1320, 1458
BstENI CCTNNNNNAGG 1 cut(s) 877
BstF5I GGATG 7 cut(s) 139, 391, 511, 1004, 1078, 1237, 1352
BstFNI CGCG 1 cut(s) 1237
BstHHI GCGC 6 cut(s) 1101, 1237, 1285, 1398, 1403, 1525
BstKTI GATC 8 cut(s) 345, 451, 577, 777, 1062, 1336, 1362, 1506
BstMAI GTCTC 3 cut(s) 1011, 1052, 1378
BstMBI GATC 8 cut(s) 342, 448, 574, 774, 1059, 1333, 1359, 1503
BstMCI CGRYCG 1 cut(s) 229
BstMWI GCNNNNNNNGC 3 cut(s) 1112, 1291, 1398
BstNI CCWGG 2 cut(s) 1120, 1196
BstNSI RCATGY 3 cut(s) 458, 584, 1294
BstSCI CCNGG 2 cut(s) 1118, 1194
BstUI CGCG 1 cut(s) 1237
BstV1I GCAGC 4 cut(s) 706, 841, 1127, 1385
BstZI CGGCCG 1 cut(s) 226
BsuRI GGCC 2 cut(s) 5, 228
BtgZI GCGATG 1 cut(s) 700
BtsCI GGATG 7 cut(s) 139, 391, 511, 1004, 1078, 1237, 1352
BtsI GCAGTG 2 cut(s) 944, 1230
BtsIMutI CAGTG 3 cut(s) 944, 1230, 1555
Cac8I GCNNGC 7 cut(s) 630, 1239, 1281, 1317, 1394, 1413, 1417
CaiI CAGNNNCTG 1 cut(s) 97
CfoI GCGC 6 cut(s) 1101, 1237, 1285, 1398, 1403, 1525
CsiI ACCWGGT 1 cut(s) 1194
Csp6I GTAC 3 cut(s) 349, 969, 1171
CspCI CAANNNNNGTGG 2 cut(s) 982, 1017
CviAII CATG 8 cut(s) 311, 377, 455, 581, 779, 1267, 1291, 1544
CviQI GTAC 3 cut(s) 349, 969, 1171
DdeI CTNAG 3 cut(s) 277, 1320, 1458
DpnI GATC 8 cut(s) 344, 450, 576, 776, 1061, 1335, 1361, 1505
DpnII GATC 8 cut(s) 342, 448, 574, 774, 1059, 1333, 1359, 1503
EaeI YGGCCR 1 cut(s) 226
EagI CGGCCG 1 cut(s) 226
Eam1104I CTCTTC 3 cut(s) 375, 495, 587
EarI CTCTTC 3 cut(s) 375, 495, 587
Ecl136II GAGCTC 1 cut(s) 203
EclXI CGGCCG 1 cut(s) 226
Eco24I GRGCYC 1 cut(s) 205
Eco31I GGTCTC 1 cut(s) 1378
Eco32I GATATC 2 cut(s) 103, 946
Eco52I CGGCCG 1 cut(s) 226
Eco53kI GAGCTC 1 cut(s) 203
EcoICRI GAGCTC 1 cut(s) 203
EcoNI CCTNNNNNAGG 1 cut(s) 877
EcoRII CCWGG 2 cut(s) 1118, 1194
EcoRV GATATC 2 cut(s) 103, 946
EcoT38I GRGCYC 1 cut(s) 205
FaeI CATG 8 cut(s) 314, 380, 458, 584, 782, 1270, 1294, 1547
FalI AAGNNNNNCTT 2 cut(s) 54, 86
FatI CATG 8 cut(s) 310, 376, 454, 580, 778, 1266, 1290, 1543
FbaI TGATCA 3 cut(s) 1059, 1359, 1503
Fnu4HI GCNGC 4 cut(s) 720, 830, 1116, 1399
FokI GGATG 7 cut(s) 146, 398, 518, 1011, 1085, 1244, 1359
FriOI GRGCYC 1 cut(s) 205
Fsp4HI GCNGC 4 cut(s) 720, 830, 1116, 1399
FspBI CTAG 3 cut(s) 657, 1179, 1377
FspI TGCGCA 1 cut(s) 1100
GlaI GCGC 6 cut(s) 1100, 1236, 1284, 1397, 1402, 1524
GluI GCNGC 4 cut(s) 720, 830, 1116, 1399
HaeIII GGCC 2 cut(s) 5, 228
HapII CCGG 1 cut(s) 31
HhaI GCGC 6 cut(s) 1101, 1237, 1285, 1398, 1403, 1525
Hin1II CATG 8 cut(s) 314, 380, 458, 584, 782, 1270, 1294, 1547
Hin6I GCGC 6 cut(s) 1099, 1235, 1283, 1396, 1401, 1523
HinP1I GCGC 6 cut(s) 1099, 1235, 1283, 1396, 1401, 1523
HinfI GANTC 4 cut(s) 41, 88, 923, 1150
HpaII CCGG 1 cut(s) 31
HphI GGTGA 3 cut(s) 988, 1016, 1460
Hpy188I TCNGA 5 cut(s) 19, 40, 93, 950, 1016
Hpy188III TCNNGA 7 cut(s) 842, 902, 1030, 1154, 1206, 1384, 1507
HpyAV CCTTC 9 cut(s) 426, 546, 634, 873, 1063, 1213, 1222, 1324, 1472
HpyCH4III ACNGT 2 cut(s) 141, 1424
HpyCH4V TGCA 9 cut(s) 119, 741, 818, 829, 837, 1115, 1294, 1415, 1559
HpyF10VI GCNNNNNNNGC 3 cut(s) 1112, 1291, 1398
HpyF3I CTNAG 3 cut(s) 277, 1320, 1458
Hsp92II CATG 8 cut(s) 314, 380, 458, 584, 782, 1270, 1294, 1547
HspAI GCGC 6 cut(s) 1099, 1235, 1283, 1396, 1401, 1523
Ksp22I TGATCA 3 cut(s) 1059, 1359, 1503
Kzo9I GATC 8 cut(s) 342, 448, 574, 774, 1059, 1333, 1359, 1503
Lsp1109I GCAGC 4 cut(s) 706, 841, 1127, 1385
LweI GCATC 3 cut(s) 941, 1222, 1510
MabI ACCWGGT 1 cut(s) 1194
MaeI CTAG 3 cut(s) 657, 1179, 1377
MalI GATC 8 cut(s) 344, 450, 576, 776, 1061, 1335, 1361, 1505
MboI GATC 8 cut(s) 342, 448, 574, 774, 1059, 1333, 1359, 1503
MhlI GDGCHC 1 cut(s) 205
MmeI TCCRAC 2 cut(s) 18, 890
MroXI GAANNNNTTC 1 cut(s) 56
MseI TTAA 8 cut(s) 260, 459, 585, 624, 708, 1082, 1188, 1368
MslI CAYNNNNRTG 1 cut(s) 1548
MspA1I CMGCKG 1 cut(s) 170
MspCI CTTAAG 1 cut(s) 1367
MspI CCGG 1 cut(s) 31
MspR9I CCNGG 2 cut(s) 1120, 1196
MteI GCGCNGCGC 1 cut(s) 1399
Mva1269I GAATGC 1 cut(s) 657
MvaI CCWGG 2 cut(s) 1120, 1196
MvnI CGCG 1 cut(s) 1237
MwoI GCNNNNNNNGC 3 cut(s) 1112, 1291, 1398
NdeII GATC 8 cut(s) 342, 448, 574, 774, 1059, 1333, 1359, 1503
NlaIII CATG 8 cut(s) 314, 380, 458, 584, 782, 1270, 1294, 1547
NlaIV GGNNCC 1 cut(s) 1165
NsbI TGCGCA 1 cut(s) 1100
NspI RCATGY 3 cut(s) 458, 584, 1294
PciI ACATGT 2 cut(s) 454, 580
PctI GAATGC 1 cut(s) 657
PdmI GAANNNNTTC 1 cut(s) 56
PfeI GAWTC 4 cut(s) 41, 88, 923, 1150
PflMI CCANNNNNTGG 1 cut(s) 1426
PkrI GCNGC 4 cut(s) 721, 831, 1117, 1400
PscI ACATGT 2 cut(s) 454, 580
Psp124BI GAGCTC 1 cut(s) 205
Psp6I CCWGG 2 cut(s) 1118, 1194
PspGI CCWGG 2 cut(s) 1118, 1194
PspN4I GGNNCC 1 cut(s) 1165
PstNI CAGNNNCTG 1 cut(s) 97
PvuII CAGCTG 1 cut(s) 170
RsaI GTAC 3 cut(s) 350, 970, 1172
RsaNI GTAC 3 cut(s) 349, 969, 1171
RseI CAYNNNNRTG 1 cut(s) 1548
SacI GAGCTC 1 cut(s) 205
SaqAI TTAA 8 cut(s) 260, 459, 585, 624, 708, 1082, 1188, 1368
SatI GCNGC 4 cut(s) 720, 830, 1116, 1399
Sau3AI GATC 8 cut(s) 342, 448, 574, 774, 1059, 1333, 1359, 1503
ScrFI CCNGG 2 cut(s) 1120, 1196
SduI GDGCHC 1 cut(s) 205
SexAI ACCWGGT 1 cut(s) 1194
SfaNI GCATC 3 cut(s) 941, 1222, 1510
SmiMI CAYNNNNRTG 1 cut(s) 1548
SmlI CTYRAG 1 cut(s) 1367
SmoI CTYRAG 1 cut(s) 1367
SspI AATATT 3 cut(s) 251, 702, 1539
SspMI CTAG 3 cut(s) 657, 1179, 1377
SstI GAGCTC 1 cut(s) 205
StyD4I CCNGG 2 cut(s) 1118, 1194
TaaI ACNGT 2 cut(s) 141, 1424
TaqI TCGA 2 cut(s) 302, 345
TatI WGTACW 2 cut(s) 968, 1170
TfiI GAWTC 4 cut(s) 41, 88, 923, 1150
Tru1I TTAA 8 cut(s) 260, 459, 585, 624, 708, 1082, 1188, 1368
Tru9I TTAA 8 cut(s) 260, 459, 585, 624, 708, 1082, 1188, 1368
TscAI CASTG 3 cut(s) 944, 1230, 1555
TseI GCWGC 4 cut(s) 719, 829, 1115, 1398
TspDTI ATGAA 4 cut(s) 150, 393, 945, 1255
TspRI CASTG 3 cut(s) 944, 1230, 1555
Van91I CCANNNNNTGG 1 cut(s) 1426
Vha464I CTTAAG 1 cut(s) 1367
XagI CCTNNNNNAGG 1 cut(s) 877
XapI RAATTY 9 cut(s) 22, 52, 125, 239, 298, 668, 1007, 1085, 1451
XceI RCATGY 3 cut(s) 458, 584, 1294
XmnI GAANNNNTTC 1 cut(s) 56
XspI CTAG 3 cut(s) 657, 1179, 1377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.