Rh2AG426000

Inorganic pyrophosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0003554
Physical Location & Seq
Forward (+)
63310584 .. 63313551
2968 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG426000.1

Sequence Viewer

Length: 726 bp
ATGCATGTGAGACTGAATTTTTGTTTTTTGGACAAAATTTTTCAGGATAGGATGATGAAGGAGCTTCATTCACAAGGAAAAACGATTGAGGACATTGTGGAGGTGCTCAAAAGGACTCCAATTCATCCCAGAATTGTGCCAGCCATTAAAGCAGCCCATGCTTTAGGATGTGACTTGAGGATTGTGAGTGATGCGAATCTGTTTTTCATTGAAACGATTTTGAAGCATCTTGGTCTGGAGGAATATTTCTCAGAAATCAACACTAACCCAGGTTCTGTTGATGAAGAAGGAAGGGTTAGGATTTCACCTTTCTGTGATTTCACCAATTCACCTCATGGCTGCAGCCTCTGCCCTCCTAACATGTGCAAGAGTGTGATAATTGAGAGAATCCAAGCTTCGACTGAAGGGAGGAAAAGGATGATCTACTTGGGTGATGGAGTTGGTGATTACTGCCCAAGCTTGAAGCTCAAGGAAGGAGATTTCGTAATGCCAAGGAAGAATTTCCCACTTTTTGATAAGATTTGCCAGAATCCTCTGGCTCTGAAGGCCGAGATTCATGAGTGGACTGATGGAGGAGAACTTGAGCACATTTTGCTCAACCTGATCAACACAATCGTCGTGGAAGAGAGTGCTGCTGCTCAGTTCATTACGACTGACTGCAAGTTACAGACCATCTCTGTTTCTGGTCATGAGGCCTTCCCTAAAGCTCTCCCAGTTCAGCAGTAG

Protein Analysis

241

Amino Acids

27.12

Weight (kDa)

5.85

Isoelectric Point (pI)

39.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Put_Phosphatase PF06888 14 - 202 5e-73 Putative Phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 3 cut(s) 16, 36, 499
AcuI CTGAAG 2 cut(s) 423, 563
AflIII ACRYGT 1 cut(s) 360
AgsI TTSAA 3 cut(s) 212, 223, 463
AjnI CCWGG 1 cut(s) 268
AluBI AGCT 5 cut(s) 64, 395, 459, 466, 707
AluI AGCT 5 cut(s) 64, 395, 459, 466, 707
Alw21I GWGCWC 2 cut(s) 108, 588
Alw26I GTCTC 1 cut(s) 4
AlwNI CAGNNNCTG 2 cut(s) 275, 348
AoxI GGCC 2 cut(s) 546, 693
ApeKI GCWGC 5 cut(s) 152, 339, 342, 632, 635
ApoI RAATTY 3 cut(s) 16, 36, 499
ArsI GACNNNNNNTTYG 1 cut(s) 35
Asp700I GAANNNNTTC 1 cut(s) 500
AsuHPI GGTGA 5 cut(s) 297, 313, 321, 443, 455
Bbv12I GWGCWC 2 cut(s) 108, 588
BbvI GCAGC 5 cut(s) 164, 326, 354, 619, 622
BccI CCATC 3 cut(s) 428, 563, 680
BciT130I CCWGG 1 cut(s) 270
BclI TGATCA 1 cut(s) 603
BcoDI GTCTC 1 cut(s) 4
BfmI CTRYAG 1 cut(s) 340
BisI GCNGC 5 cut(s) 153, 340, 343, 633, 636
BlsI GCNGC 5 cut(s) 154, 341, 344, 634, 637
Bme1390I CCNGG 1 cut(s) 270
BmrFI CCNGG 1 cut(s) 270
BmrI ACTGGG 1 cut(s) 707
BmsI GCATC 2 cut(s) 181, 235
BmuI ACTGGG 1 cut(s) 707
BpmI CTGGAG 1 cut(s) 257
BpuEI CTTGAG 3 cut(s) 196, 452, 602
BsaBI GATNNNNATC 1 cut(s) 195
BsaJI CCNNGG 2 cut(s) 268, 491
BsaXI ACNNNNNCTCC 2 cut(s) 468, 498
Bse1I ACTGG 1 cut(s) 713
Bse8I GATNNNNATC 1 cut(s) 195
BseBI CCWGG 1 cut(s) 270
BseDI CCNNGG 2 cut(s) 268, 491
BseGI GGATG 4 cut(s) 57, 124, 173, 423
BseJI GATNNNNATC 1 cut(s) 195
BseMII CTCAG 2 cut(s) 264, 653
BseNI ACTGG 1 cut(s) 713
BseRI GAGGAG 1 cut(s) 588
BseXI GCAGC 5 cut(s) 164, 326, 354, 619, 622
BshFI GGCC 2 cut(s) 548, 695
BsiHKAI GWGCWC 2 cut(s) 108, 588
BsmAI GTCTC 1 cut(s) 4
BsnI GGCC 2 cut(s) 548, 695
Bsp1286I GDGCHC 2 cut(s) 108, 588
Bsp143I GATC 2 cut(s) 420, 603
BspANI GGCC 2 cut(s) 548, 695
BspCNI CTCAG 2 cut(s) 263, 652
BspHI TCATGA 2 cut(s) 556, 688
BspMAI CTGCAG 1 cut(s) 344
BsrI ACTGG 1 cut(s) 713
BssECI CCNNGG 2 cut(s) 268, 491
BssMI GATC 2 cut(s) 420, 603
BssT1I CCWWGG 1 cut(s) 491
Bst2UI CCWGG 1 cut(s) 270
Bst6I CTCTTC 1 cut(s) 618
BstAPI GCANNNNNTGC 3 cut(s) 158, 348, 592
BstC8I GCNNGC 1 cut(s) 141
BstDEI CTNAG 2 cut(s) 250, 639
BstF5I GGATG 4 cut(s) 57, 124, 173, 423
BstKTI GATC 2 cut(s) 423, 606
BstMAI GTCTC 1 cut(s) 4
BstMBI GATC 2 cut(s) 420, 603
BstMWI GCNNNNNNNGC 5 cut(s) 149, 158, 348, 545, 592
BstNI CCWGG 1 cut(s) 270
BstNSI RCATGY 2 cut(s) 8, 364
BstSCI CCNGG 1 cut(s) 268
BstSFI CTRYAG 1 cut(s) 340
BstV1I GCAGC 5 cut(s) 164, 326, 354, 619, 622
BsuRI GGCC 2 cut(s) 548, 695
BtsCI GGATG 4 cut(s) 57, 124, 173, 423
Cac8I GCNNGC 1 cut(s) 141
CaiI CAGNNNCTG 2 cut(s) 275, 348
CciI TCATGA 2 cut(s) 556, 688
CspCI CAANNNNNGTGG 2 cut(s) 600, 635
CviAII CATG 6 cut(s) 5, 158, 335, 361, 557, 689
DdeI CTNAG 2 cut(s) 250, 639
DpnI GATC 2 cut(s) 422, 605
DpnII GATC 2 cut(s) 420, 603
Eam1104I CTCTTC 1 cut(s) 618
EarI CTCTTC 1 cut(s) 618
Eco130I CCWWGG 1 cut(s) 491
Eco147I AGGCCT 1 cut(s) 695
Eco57I CTGAAG 2 cut(s) 423, 563
EcoRII CCWGG 1 cut(s) 268
EcoT14I CCWWGG 1 cut(s) 491
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 491
FaeI CATG 6 cut(s) 8, 161, 338, 364, 560, 692
FaiI YATR 6 cut(s) 6, 159, 336, 362, 558, 690
FatI CATG 6 cut(s) 4, 157, 334, 360, 556, 688
FbaI TGATCA 1 cut(s) 603
Fnu4HI GCNGC 5 cut(s) 153, 340, 343, 633, 636
FokI GGATG 4 cut(s) 64, 111, 180, 430
Fsp4HI GCNGC 5 cut(s) 153, 340, 343, 633, 636
GluI GCNGC 5 cut(s) 153, 340, 343, 633, 636
GsuI CTGGAG 1 cut(s) 257
HaeIII GGCC 2 cut(s) 548, 695
Hin1II CATG 6 cut(s) 8, 161, 338, 364, 560, 692
HindIII AAGCTT 2 cut(s) 393, 457
HinfI GANTC 5 cut(s) 115, 196, 387, 529, 553
HphI GGTGA 5 cut(s) 297, 313, 321, 443, 455
Hpy166II GTNNAC 1 cut(s) 564
Hpy188I TCNGA 2 cut(s) 253, 543
Hpy188III TCNNGA 4 cut(s) 44, 236, 557, 689
Hpy8I GTNNAC 1 cut(s) 564
Hpy99I CGWCG 1 cut(s) 620
HpyAV CCTTC 7 cut(s) 52, 281, 285, 398, 467, 538, 706
HpyCH4V TGCA 4 cut(s) 4, 342, 366, 660
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 158, 348, 545, 592
HpyF3I CTNAG 2 cut(s) 250, 639
Hsp92II CATG 6 cut(s) 8, 161, 338, 364, 560, 692
Ksp22I TGATCA 1 cut(s) 603
Kzo9I GATC 2 cut(s) 420, 603
LmnI GCTCC 1 cut(s) 61
Lsp1109I GCAGC 5 cut(s) 164, 326, 354, 619, 622
LweI GCATC 2 cut(s) 181, 235
MaeIII GTNAC 2 cut(s) 170, 663
MalI GATC 2 cut(s) 422, 605
MboI GATC 2 cut(s) 420, 603
MboII GAAGA 3 cut(s) 296, 508, 635
MhlI GDGCHC 2 cut(s) 108, 588
MluCI AATT 7 cut(s) 16, 36, 120, 132, 325, 378, 499
MlyI GAGTC 1 cut(s) 109
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 500
MseI TTAA 1 cut(s) 147
MspR9I CCNGG 1 cut(s) 270
MvaI CCWGG 1 cut(s) 270
MwoI GCNNNNNNNGC 5 cut(s) 149, 158, 348, 545, 592
NdeII GATC 2 cut(s) 420, 603
NlaIII CATG 6 cut(s) 8, 161, 338, 364, 560, 692
NmeAIII GCCGAG 1 cut(s) 574
NmuCI GTSAC 1 cut(s) 170
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 8, 364
PagI TCATGA 2 cut(s) 556, 688
PceI AGGCCT 1 cut(s) 695
PciI ACATGT 1 cut(s) 360
PdmI GAANNNNTTC 1 cut(s) 500
PfeI GAWTC 4 cut(s) 196, 387, 529, 553
PkrI GCNGC 5 cut(s) 154, 341, 344, 634, 637
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PscI ACATGT 1 cut(s) 360
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PstI CTGCAG 1 cut(s) 344
PstNI CAGNNNCTG 2 cut(s) 275, 348
SaqAI TTAA 1 cut(s) 147
SatI GCNGC 5 cut(s) 153, 340, 343, 633, 636
Sau3AI GATC 2 cut(s) 420, 603
SchI GAGTC 1 cut(s) 109
ScrFI CCNGG 1 cut(s) 270
SduI GDGCHC 2 cut(s) 108, 588
SfaNI GCATC 2 cut(s) 181, 235
SfcI CTRYAG 1 cut(s) 340
SmlI CTYRAG 3 cut(s) 175, 467, 581
SmoI CTYRAG 3 cut(s) 175, 467, 581
Sse9I AATT 7 cut(s) 16, 36, 120, 132, 325, 378, 499
SseBI AGGCCT 1 cut(s) 695
SspI AATATT 1 cut(s) 245
StuI AGGCCT 1 cut(s) 695
StyD4I CCNGG 1 cut(s) 268
StyI CCWWGG 1 cut(s) 491
TaqI TCGA 1 cut(s) 398
TasI AATT 7 cut(s) 16, 36, 120, 132, 325, 378, 499
TfiI GAWTC 4 cut(s) 196, 387, 529, 553
Tru1I TTAA 1 cut(s) 147
Tru9I TTAA 1 cut(s) 147
TseFI GTSAC 1 cut(s) 170
TseI GCWGC 5 cut(s) 152, 339, 342, 632, 635
Tsp45I GTSAC 1 cut(s) 170
TspDTI ATGAA 7 cut(s) 56, 71, 113, 196, 297, 545, 634
XapI RAATTY 3 cut(s) 16, 36, 499
XceI RCATGY 2 cut(s) 8, 364
XmnI GAANNNNTTC 1 cut(s) 500
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.