Rh2BG440000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0006963
Physical Location & Seq
Forward (+)
62359963 .. 62360418
456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG440000.1

Sequence Viewer

Length: 456 bp
ATGAGGTCCTCTGACATATTCCAGACTCATGTAAATGCAAGACGAATCAAAAGTTTCCAACAGCTTATTAAAGCTATGGCCACTGCTGAAAGACATGGCAATGAACTTGTGAAAAGAGAAATTGATAGGCTTCAAGATAGCCATCAAGAGCATCGTACTATGGCTAGAGAAAGTTGCCATCGACGTCATGATCCATACGATCAAAATGCTCAAGAAGGTAATCGCTCAAGGCGACAATCACACAACCATAGGAGGCATGATTTTGAGGGACATAAGGTTGGAAACCATGGAGCCAACGTTGGCCATGGTCACCACTTTCCAACGCCACCATTCCTAGGGACTATGCATATTGCGCCAATGCGCTTCAATCAAGGGAGAATCCTCTTTATGCTGTCTATTTCTGATATGGAACGTTTGATCAATGGACCTGAATTTGCAATGTACCTACGAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

151

Amino Acids

17.8

Weight (kDa)

10.72

Isoelectric Point (pI)

73.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 187
AclI AACGTT 2 cut(s) 297, 412
AclWI GGATC 1 cut(s) 185
AcoI YGGCCR 2 cut(s) 78, 301
AcsI RAATTY 1 cut(s) 431
AcyI GRCGYC 1 cut(s) 184
AfaI GTAC 2 cut(s) 157, 443
AfiI CCNNNNNNNGG 1 cut(s) 335
AgsI TTSAA 2 cut(s) 134, 367
AluBI AGCT 2 cut(s) 64, 74
AluI AGCT 2 cut(s) 64, 74
AlwI GGATC 1 cut(s) 185
AoxI GGCC 2 cut(s) 78, 301
ApoI RAATTY 1 cut(s) 431
AspA2I CCTAGG 1 cut(s) 334
AspLEI GCGC 2 cut(s) 355, 363
AspS9I GGNCC 2 cut(s) 6, 425
AsuHPI GGTGA 1 cut(s) 302
AvaII GGWCC 2 cut(s) 6, 425
AvrII CCTAGG 1 cut(s) 334
BalI TGGCCA 2 cut(s) 80, 303
BccI CCATC 2 cut(s) 150, 186
BcgI CGANNNNNNTGC 2 cut(s) 188, 222
BclI TGATCA 1 cut(s) 417
BfaI CTAG 2 cut(s) 165, 335
BlnI CCTAGG 1 cut(s) 334
Bme18I GGWCC 2 cut(s) 6, 425
BmgT120I GGNCC 2 cut(s) 6, 425
BmiI GGNNCC 1 cut(s) 292
BmsI GCATC 1 cut(s) 160
BpuEI CTTGAG 2 cut(s) 195, 211
BsaBI GATNNNNATC 1 cut(s) 141
BsaHI GRCGYC 1 cut(s) 184
BsaJI CCNNGG 3 cut(s) 286, 304, 334
BsaXI ACNNNNNCTCC 2 cut(s) 282, 312
Bsc4I CCNNNNNNNGG 1 cut(s) 335
Bse3DI GCAATG 2 cut(s) 106, 444
Bse8I GATNNNNATC 1 cut(s) 141
BseDI CCNNGG 3 cut(s) 286, 304, 334
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 1 cut(s) 335
BseMI GCAATG 2 cut(s) 106, 444
BshFI GGCC 2 cut(s) 80, 303
BslFI GGGAC 2 cut(s) 282, 352
BslI CCNNNNNNNGG 1 cut(s) 335
BsmFI GGGAC 2 cut(s) 282, 352
BsnI GGCC 2 cut(s) 80, 303
Bsp143I GATC 3 cut(s) 190, 199, 417
Bsp19I CCATGG 2 cut(s) 286, 304
BspANI GGCC 2 cut(s) 80, 303
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 1 cut(s) 292
BspPI GGATC 1 cut(s) 185
BsrDI GCAATG 2 cut(s) 106, 444
BssECI CCNNGG 3 cut(s) 286, 304, 334
BssMI GATC 3 cut(s) 190, 199, 417
BssNI GRCGYC 1 cut(s) 184
BssT1I CCWWGG 3 cut(s) 286, 304, 334
BstACI GRCGYC 1 cut(s) 184
BstDSI CCRYGG 2 cut(s) 286, 304
BstEII GGTNACC 1 cut(s) 308
BstHHI GCGC 2 cut(s) 355, 363
BstKTI GATC 3 cut(s) 193, 202, 420
BstMBI GATC 3 cut(s) 190, 199, 417
BstMWI GCNNNNNNNGC 1 cut(s) 352
BstPI GGTNACC 1 cut(s) 308
BsuRI GGCC 2 cut(s) 80, 303
BtgI CCRYGG 2 cut(s) 286, 304
BtsI GCAGTG 1 cut(s) 81
BtsIMutI CAGTG 1 cut(s) 81
CciI TCATGA 1 cut(s) 187
CfoI GCGC 2 cut(s) 355, 363
Cfr13I GGNCC 2 cut(s) 6, 425
Csp6I GTAC 2 cut(s) 156, 442
CviAII CATG 6 cut(s) 29, 95, 188, 257, 287, 305
CviJI RGCY 8 cut(s) 64, 74, 80, 130, 141, 164, 293, 303
CviKI_1 RGCY 8 cut(s) 64, 74, 80, 130, 141, 164, 293, 303
CviQI GTAC 2 cut(s) 156, 442
DpnI GATC 3 cut(s) 192, 201, 419
DpnII GATC 3 cut(s) 190, 199, 417
EaeI YGGCCR 2 cut(s) 78, 301
Eco130I CCWWGG 3 cut(s) 286, 304, 334
Eco47I GGWCC 2 cut(s) 6, 425
Eco91I GGTNACC 1 cut(s) 308
EcoO109I RGGNCCY 1 cut(s) 6
EcoO65I GGTNACC 1 cut(s) 308
EcoT14I CCWWGG 3 cut(s) 286, 304, 334
EcoT22I ATGCAT 1 cut(s) 348
ErhI CCWWGG 3 cut(s) 286, 304, 334
FaeI CATG 6 cut(s) 32, 98, 191, 260, 290, 308
FaqI GGGAC 2 cut(s) 282, 352
FatI CATG 6 cut(s) 28, 94, 187, 256, 286, 304
FbaI TGATCA 1 cut(s) 417
FspBI CTAG 2 cut(s) 165, 335
GlaI GCGC 2 cut(s) 354, 362
HaeIII GGCC 2 cut(s) 80, 303
HhaI GCGC 2 cut(s) 355, 363
Hin1I GRCGYC 1 cut(s) 184
Hin1II CATG 6 cut(s) 32, 98, 191, 260, 290, 308
Hin6I GCGC 2 cut(s) 353, 361
HinP1I GCGC 2 cut(s) 353, 361
HinfI GANTC 3 cut(s) 25, 45, 378
HphI GGTGA 1 cut(s) 302
Hpy188I TCNGA 2 cut(s) 13, 403
Hpy188III TCNNGA 5 cut(s) 22, 134, 146, 188, 212
Hpy99I CGWCG 1 cut(s) 186
HpyAV CCTTC 2 cut(s) 209, 444
HpyCH4IV ACGT 3 cut(s) 184, 297, 412
HpyCH4V TGCA 3 cut(s) 38, 346, 437
HpyF10VI GCNNNNNNNGC 1 cut(s) 352
HpySE526I ACGT 3 cut(s) 184, 297, 412
Hsp92I GRCGYC 1 cut(s) 184
Hsp92II CATG 6 cut(s) 32, 98, 191, 260, 290, 308
HspAI GCGC 2 cut(s) 353, 361
Ksp22I TGATCA 1 cut(s) 417
Kzo9I GATC 3 cut(s) 190, 199, 417
LmnI GCTCC 1 cut(s) 290
LpnPI CCDG 2 cut(s) 35, 441
LweI GCATC 1 cut(s) 160
MaeI CTAG 2 cut(s) 165, 335
MaeII ACGT 3 cut(s) 184, 297, 412
MaeIII GTNAC 1 cut(s) 308
MalI GATC 3 cut(s) 192, 201, 419
MboI GATC 3 cut(s) 190, 199, 417
MlsI TGGCCA 2 cut(s) 80, 303
MluCI AATT 2 cut(s) 120, 431
MluNI TGGCCA 2 cut(s) 80, 303
MlyI GAGTC 1 cut(s) 19
MmeI TCCRAC 3 cut(s) 82, 259, 344
MnlI CCTC 4 cut(s) 19, 246, 259, 392
Mox20I TGGCCA 2 cut(s) 80, 303
Mph1103I ATGCAT 1 cut(s) 348
MscI TGGCCA 2 cut(s) 80, 303
MseI TTAA 1 cut(s) 69
MslI CAYNNNNRTG 2 cut(s) 33, 99
Msp20I TGGCCA 2 cut(s) 80, 303
MwoI GCNNNNNNNGC 1 cut(s) 352
NcoI CCATGG 2 cut(s) 286, 304
NdeII GATC 3 cut(s) 190, 199, 417
NlaIII CATG 6 cut(s) 32, 98, 191, 260, 290, 308
NlaIV GGNNCC 1 cut(s) 292
NmuCI GTSAC 1 cut(s) 308
NsiI ATGCAT 1 cut(s) 348
PagI TCATGA 1 cut(s) 187
PcsI WCGNNNNNNNCGW 1 cut(s) 229
PfeI GAWTC 2 cut(s) 45, 378
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
PpuMI RGGWCCY 1 cut(s) 6
Psp1406I AACGTT 2 cut(s) 297, 412
Psp5II RGGWCCY 1 cut(s) 6
PspEI GGTNACC 1 cut(s) 308
PspN4I GGNNCC 1 cut(s) 292
PspPI GGNCC 2 cut(s) 6, 425
PspPPI RGGWCCY 1 cut(s) 6
RsaI GTAC 2 cut(s) 157, 443
RsaNI GTAC 2 cut(s) 156, 442
RseI CAYNNNNRTG 2 cut(s) 33, 99
SaqAI TTAA 1 cut(s) 69
Sau3AI GATC 3 cut(s) 190, 199, 417
Sau96I GGNCC 2 cut(s) 6, 425
SchI GAGTC 1 cut(s) 19
SfaNI GCATC 1 cut(s) 160
SinI GGWCC 2 cut(s) 6, 425
SmiMI CAYNNNNRTG 2 cut(s) 33, 99
SmlI CTYRAG 2 cut(s) 210, 226
SmoI CTYRAG 2 cut(s) 210, 226
Sse9I AATT 2 cut(s) 120, 431
SspMI CTAG 2 cut(s) 165, 335
StyI CCWWGG 3 cut(s) 286, 304, 334
TaiI ACGT 3 cut(s) 187, 300, 415
TaqI TCGA 1 cut(s) 181
TasI AATT 2 cut(s) 120, 431
TfiI GAWTC 2 cut(s) 45, 378
Tru1I TTAA 1 cut(s) 69
Tru9I TTAA 1 cut(s) 69
TscAI CASTG 1 cut(s) 88
TseFI GTSAC 1 cut(s) 308
Tsp45I GTSAC 1 cut(s) 308
TspDTI ATGAA 1 cut(s) 117
TspRI CASTG 1 cut(s) 88
VpaK11BI GGWCC 2 cut(s) 6, 425
XapI RAATTY 1 cut(s) 431
XmaJI CCTAGG 1 cut(s) 334
XspI CTAG 2 cut(s) 165, 335
ZraI GACGTC 1 cut(s) 185
Zsp2I ATGCAT 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.