Rh2DG630500

Converts alpha-aldose to the beta-anomer

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004802
Physical Location & Seq
Reverse (-)
85984941 .. 85987655
2715 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG630500.1

Sequence Viewer

Length: 891 bp
ATGACTGAACTTGTAATGGTAATTCTGCAGAATGATACTTCATACATCGGTGCTATTGTTGGACGCGTTGCTAACAGAATCGGTGGCGCTCAATTTACTTTGAATGGAACCCATTACAAGCTAGTTGCTAATGAAGGAAAAAACATGCTTCATGGTGGGCGTAGAGGATTTAGTGATGTTGTTTGGAAAGTGAAGAAGTATAAGAATGAAGGTCATACTCCTTCCATTGTCTTCACTTATCATAGTGCTGATGGGGAACAAGGGTTTCCTGGAGATGTTATTGTCACTGTCAGCTACACACTCCTTGGAGACAAACAATTGATCGTGAAAATGAAAGCAAAGGCCCTAAACAAGCCAACTCCAGTGAATCTAGCTCAGCACACTTACTGGAACCTTGGCGGCCACAATAGTGGTGACATTCTCTCCCAACACATCCAGATCTTTGCATCCAAAATCACTCTGGTAGATAGCGGTCTCATCCCCACCGGAAAATTTGCATCCGTGAAAGGCACACCCTATGATTTCCTCAAGCCTCAAGCAGTTGGAAGCAGGATCAACAAGCTTACGGCAACCAAAGGTTATGACATCAACTACGTGCTTGATGGCCCAGCTGGCAACAAGTTGAAGCGCGCCGCAGTGGTGCATGACAGCAAGTCAGGAAGGATGATGGAGCTCTCAACAAATGTTCCCGGTATGCAGTTCTACACAAGCAACTATCTCCAGGATGTGAAGGGGAAAGGTGGGTTTGTATACCAACCACGTGCAGCATTGTGTTTGGAAACTCAGGGATTTCCTGATGCTGTGAACCACCCTAATTTCCCTTCAACAATTGTGACTTCAACAAAGTCCTATAGGCATTATATGCTGTTCAAGTTTTCAACTACAGCTTAA

Protein Analysis

296

Amino Acids

32.42

Weight (kDa)

9.67

Isoelectric Point (pI)

26.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldose_epim PF01263 10 - 290 6.2e-77 Aldose 1-epimerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 750
AccII CGCG 2 cut(s) 66, 630
AciI CCGC 3 cut(s) 399, 471, 633
AclWI GGATC 1 cut(s) 560
AcoI YGGCCR 1 cut(s) 400
AcsI RAATTY 1 cut(s) 491
AcvI CACGTG 1 cut(s) 761
AflIII ACRYGT 1 cut(s) 64
AgsI TTSAA 6 cut(s) 103, 625, 825, 840, 871, 879
AhdI GACNNNNNGTC 1 cut(s) 652
AjnI CCWGG 2 cut(s) 268, 720
AleI CACNNNNGTG 1 cut(s) 408
AluBI AGCT 7 cut(s) 121, 294, 374, 562, 611, 673, 887
AluI AGCT 7 cut(s) 121, 294, 374, 562, 611, 673, 887
Alw21I GWGCWC 1 cut(s) 675
Alw26I GTCTC 2 cut(s) 303, 479
AlwI GGATC 1 cut(s) 560
AoxI GGCC 3 cut(s) 342, 400, 604
ApeKI GCWGC 1 cut(s) 764
ApoI RAATTY 1 cut(s) 491
AspLEI GCGC 3 cut(s) 89, 630, 632
AspS9I GGNCC 2 cut(s) 343, 605
AsuC2I CCSGG 1 cut(s) 690
AsuHPI GGTGA 1 cut(s) 425
BanII GRGCYC 1 cut(s) 675
BbrPI CACGTG 1 cut(s) 761
BbsI GAAGAC 1 cut(s) 223
Bbv12I GWGCWC 1 cut(s) 675
BbvI GCAGC 1 cut(s) 776
BccI CCATC 3 cut(s) 245, 596, 661
BceAI ACGGC 1 cut(s) 582
BciT130I CCWGG 2 cut(s) 270, 722
BcnI CCSGG 1 cut(s) 690
BcoDI GTCTC 2 cut(s) 303, 479
BfaI CTAG 2 cut(s) 122, 371
BfmI CTRYAG 3 cut(s) 26, 850, 882
BfoI RGCGCY 1 cut(s) 90
BglI GCCNNNNNGGC 1 cut(s) 612
BglII AGATCT 1 cut(s) 438
BisI GCNGC 3 cut(s) 400, 633, 765
BlpI GCTNAGC 1 cut(s) 375
BlsI GCNGC 3 cut(s) 401, 634, 766
Bme1390I CCNGG 3 cut(s) 270, 690, 722
BmeRI GACNNNNNGTC 1 cut(s) 652
BmgT120I GGNCC 2 cut(s) 343, 605
BmiI GGNNCC 2 cut(s) 109, 392
BmrFI CCNGG 3 cut(s) 270, 690, 722
BmsI GCATC 3 cut(s) 455, 506, 787
BpiI GAAGAC 1 cut(s) 223
BpmI CTGGAG 3 cut(s) 291, 345, 704
Bpu1102I GCTNAGC 1 cut(s) 375
BpuEI CTTGAG 2 cut(s) 512, 519
BpuMI CCSGG 1 cut(s) 690
BsaAI YACGTR 2 cut(s) 595, 761
BsaI GGTCTC 1 cut(s) 479
BsaJI CCNNGG 2 cut(s) 304, 394
BsaWI WCCGGW 1 cut(s) 485
BsaXI ACNNNNNCTCC 2 cut(s) 407, 437
Bse1I ACTGG 2 cut(s) 362, 392
BseBI CCWGG 2 cut(s) 270, 722
BseDI CCNNGG 2 cut(s) 304, 394
BseGI GGATG 6 cut(s) 432, 446, 477, 497, 669, 730
BseMII CTCAG 2 cut(s) 389, 797
BseNI ACTGG 2 cut(s) 362, 392
BsePI GCGCGC 1 cut(s) 628
BseXI GCAGC 1 cut(s) 776
BseYI CCCAGC 1 cut(s) 607
BsgI GTGCAG 1 cut(s) 783
Bsh1236I CGCG 2 cut(s) 66, 630
BshFI GGCC 3 cut(s) 344, 402, 606
BsiHKAI GWGCWC 1 cut(s) 675
BsiSI CCGG 2 cut(s) 486, 690
BsmAI GTCTC 2 cut(s) 303, 479
BsnI GGCC 3 cut(s) 344, 402, 606
Bso31I GGTCTC 1 cut(s) 479
Bsp1286I GDGCHC 1 cut(s) 675
Bsp143I GATC 3 cut(s) 321, 438, 552
Bsp1720I GCTNAGC 1 cut(s) 375
BspACI CCGC 3 cut(s) 399, 471, 633
BspANI GGCC 3 cut(s) 344, 402, 606
BspCNI CTCAG 2 cut(s) 388, 796
BspFNI CGCG 2 cut(s) 66, 630
BspLI GGNNCC 2 cut(s) 109, 392
BspMAI CTGCAG 1 cut(s) 30
BspPI GGATC 1 cut(s) 560
BspTNI GGTCTC 1 cut(s) 479
BsrI ACTGG 2 cut(s) 362, 392
BssECI CCNNGG 2 cut(s) 304, 394
BssHII GCGCGC 1 cut(s) 628
BssMI GATC 3 cut(s) 321, 438, 552
BssNAI GTATAC 1 cut(s) 751
BssT1I CCWWGG 2 cut(s) 304, 394
Bst1107I GTATAC 1 cut(s) 751
Bst2UI CCWGG 2 cut(s) 270, 722
Bst4CI ACNGT 1 cut(s) 289
BstAPI GCANNNNNTGC 1 cut(s) 862
BstBAI YACGTR 2 cut(s) 595, 761
BstC8I GCNNGC 2 cut(s) 613, 630
BstDEI CTNAG 2 cut(s) 375, 783
BstF5I GGATG 6 cut(s) 432, 446, 477, 497, 669, 730
BstFNI CGCG 2 cut(s) 66, 630
BstH2I RGCGCY 1 cut(s) 90
BstHHI GCGC 3 cut(s) 89, 630, 632
BstKTI GATC 3 cut(s) 324, 441, 555
BstMAI GTCTC 2 cut(s) 303, 479
BstMBI GATC 3 cut(s) 321, 438, 552
BstMWI GCNNNNNNNGC 2 cut(s) 612, 862
BstNI CCWGG 2 cut(s) 270, 722
BstNSI RCATGY 1 cut(s) 148
BstSCI CCNGG 3 cut(s) 268, 688, 720
BstSFI CTRYAG 3 cut(s) 26, 850, 882
BstUI CGCG 2 cut(s) 66, 630
BstV1I GCAGC 1 cut(s) 776
BstV2I GAAGAC 1 cut(s) 223
BstX2I RGATCY 1 cut(s) 438
BstXI CCANNNNNNTGG 1 cut(s) 410
BstYI RGATCY 1 cut(s) 438
BstZ17I GTATAC 1 cut(s) 751
BsuRI GGCC 3 cut(s) 344, 402, 606
BtsCI GGATG 6 cut(s) 432, 446, 477, 497, 669, 730
BtsI GCAGTG 1 cut(s) 642
BtsIMutI CAGTG 3 cut(s) 285, 369, 642
Cac8I GCNNGC 2 cut(s) 613, 630
CfoI GCGC 3 cut(s) 89, 630, 632
Cfr13I GGNCC 2 cut(s) 343, 605
CseI GACGC 1 cut(s) 72
CviAII CATG 3 cut(s) 145, 152, 644
DdeI CTNAG 2 cut(s) 375, 783
DpnI GATC 3 cut(s) 323, 440, 554
DpnII GATC 3 cut(s) 321, 438, 552
DriI GACNNNNNGTC 1 cut(s) 652
EaeI YGGCCR 1 cut(s) 400
Eam1105I GACNNNNNGTC 1 cut(s) 652
Ecl136II GAGCTC 1 cut(s) 673
Eco130I CCWWGG 2 cut(s) 304, 394
Eco24I GRGCYC 1 cut(s) 675
Eco31I GGTCTC 1 cut(s) 479
Eco53kI GAGCTC 1 cut(s) 673
Eco72I CACGTG 1 cut(s) 761
EcoICRI GAGCTC 1 cut(s) 673
EcoO109I RGGNCCY 1 cut(s) 343
EcoRII CCWGG 2 cut(s) 268, 720
EcoT14I CCWWGG 2 cut(s) 304, 394
EcoT38I GRGCYC 1 cut(s) 675
ErhI CCWWGG 2 cut(s) 304, 394
FaeI CATG 3 cut(s) 148, 155, 647
FatI CATG 3 cut(s) 144, 151, 643
FblI GTMKAC 1 cut(s) 750
Fnu4HI GCNGC 3 cut(s) 400, 633, 765
FokI GGATG 6 cut(s) 419, 433, 464, 484, 676, 737
FriOI GRGCYC 1 cut(s) 675
Fsp4HI GCNGC 3 cut(s) 400, 633, 765
FspBI CTAG 2 cut(s) 122, 371
GlaI GCGC 3 cut(s) 88, 629, 631
GluI GCNGC 3 cut(s) 400, 633, 765
GsaI CCCAGC 1 cut(s) 611
GsuI CTGGAG 3 cut(s) 291, 345, 704
HaeII RGCGCY 1 cut(s) 90
HaeIII GGCC 3 cut(s) 344, 402, 606
HapII CCGG 2 cut(s) 486, 690
HgaI GACGC 1 cut(s) 72
HhaI GCGC 3 cut(s) 89, 630, 632
Hin1II CATG 3 cut(s) 148, 155, 647
Hin6I GCGC 3 cut(s) 87, 628, 630
HinP1I GCGC 3 cut(s) 87, 628, 630
HindIII AAGCTT 1 cut(s) 560
HinfI GANTC 2 cut(s) 78, 367
HpaII CCGG 2 cut(s) 486, 690
HphI GGTGA 1 cut(s) 425
Hpy166II GTNNAC 2 cut(s) 751, 805
Hpy188III TCNNGA 4 cut(s) 325, 436, 657, 794
Hpy8I GTNNAC 2 cut(s) 751, 805
HpyAV CCTTC 6 cut(s) 128, 203, 231, 654, 724, 831
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 2 cut(s) 594, 760
HpyCH4V TGCA 6 cut(s) 28, 446, 497, 643, 697, 764
HpyF10VI GCNNNNNNNGC 2 cut(s) 612, 862
HpyF3I CTNAG 2 cut(s) 375, 783
HpySE526I ACGT 2 cut(s) 594, 760
Hsp92II CATG 3 cut(s) 148, 155, 647
HspAI GCGC 3 cut(s) 87, 628, 630
Kzo9I GATC 3 cut(s) 321, 438, 552
LmnI GCTCC 1 cut(s) 670
Lsp1109I GCAGC 1 cut(s) 776
LweI GCATC 3 cut(s) 455, 506, 787
MaeI CTAG 2 cut(s) 122, 371
MaeII ACGT 2 cut(s) 594, 760
MaeIII GTNAC 3 cut(s) 283, 413, 832
MalI GATC 3 cut(s) 323, 440, 554
MboI GATC 3 cut(s) 321, 438, 552
MboII GAAGA 2 cut(s) 205, 223
MfeI CAATTG 2 cut(s) 317, 828
MflI RGATCY 1 cut(s) 438
MhlI GDGCHC 1 cut(s) 675
MluCI AATT 6 cut(s) 21, 92, 317, 491, 814, 828
MluI ACGCGT 1 cut(s) 64
MmeI TCCRAC 2 cut(s) 40, 523
MnlI CCTC 3 cut(s) 158, 536, 543
MseI TTAA 1 cut(s) 889
MslI CAYNNNNRTG 1 cut(s) 408
MspA1I CMGCKG 1 cut(s) 611
MspI CCGG 2 cut(s) 486, 690
MspR9I CCNGG 3 cut(s) 270, 690, 722
MunI CAATTG 2 cut(s) 317, 828
MvaI CCWGG 2 cut(s) 270, 722
MvnI CGCG 2 cut(s) 66, 630
MwoI GCNNNNNNNGC 2 cut(s) 612, 862
NciI CCSGG 1 cut(s) 690
NdeII GATC 3 cut(s) 321, 438, 552
NlaIII CATG 3 cut(s) 148, 155, 647
NlaIV GGNNCC 2 cut(s) 109, 392
NmuCI GTSAC 3 cut(s) 283, 413, 832
NspI RCATGY 1 cut(s) 148
OliI CACNNNNGTG 1 cut(s) 408
PauI GCGCGC 1 cut(s) 628
PfeI GAWTC 2 cut(s) 78, 367
PfoI TCCNGGA 2 cut(s) 268, 720
PkrI GCNGC 3 cut(s) 401, 634, 766
PmaCI CACGTG 1 cut(s) 761
PmlI CACGTG 1 cut(s) 761
Ppu21I YACGTR 2 cut(s) 595, 761
Psp124BI GAGCTC 1 cut(s) 675
Psp6I CCWGG 2 cut(s) 268, 720
PspCI CACGTG 1 cut(s) 761
PspFI CCCAGC 1 cut(s) 607
PspGI CCWGG 2 cut(s) 268, 720
PspN4I GGNNCC 2 cut(s) 109, 392
PspPI GGNCC 2 cut(s) 343, 605
PstI CTGCAG 1 cut(s) 30
PsuI RGATCY 1 cut(s) 438
PteI GCGCGC 1 cut(s) 628
PvuII CAGCTG 1 cut(s) 611
RseI CAYNNNNRTG 1 cut(s) 408
SacI GAGCTC 1 cut(s) 675
SaqAI TTAA 1 cut(s) 889
SatI GCNGC 3 cut(s) 400, 633, 765
Sau3AI GATC 3 cut(s) 321, 438, 552
Sau96I GGNCC 2 cut(s) 343, 605
ScrFI CCNGG 3 cut(s) 270, 690, 722
SduI GDGCHC 1 cut(s) 675
SfaNI GCATC 3 cut(s) 455, 506, 787
SfcI CTRYAG 3 cut(s) 26, 850, 882
SmiMI CAYNNNNRTG 1 cut(s) 408
SmlI CTYRAG 2 cut(s) 527, 534
SmoI CTYRAG 2 cut(s) 527, 534
Sse9I AATT 6 cut(s) 21, 92, 317, 491, 814, 828
SsiI CCGC 3 cut(s) 399, 471, 633
SspMI CTAG 2 cut(s) 122, 371
SstI GAGCTC 1 cut(s) 675
StyD4I CCNGG 3 cut(s) 268, 688, 720
StyI CCWWGG 2 cut(s) 304, 394
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 2 cut(s) 597, 763
TasI AATT 6 cut(s) 21, 92, 317, 491, 814, 828
TauI GCSGC 2 cut(s) 402, 635
TfiI GAWTC 2 cut(s) 78, 367
Tru1I TTAA 1 cut(s) 889
Tru9I TTAA 1 cut(s) 889
TscAI CASTG 3 cut(s) 292, 369, 642
TseFI GTSAC 3 cut(s) 283, 413, 832
TseI GCWGC 1 cut(s) 764
Tsp45I GTSAC 3 cut(s) 283, 413, 832
TspDTI ATGAA 5 cut(s) 30, 140, 147, 222, 347
TspGWI ACGGA 1 cut(s) 490
TspRI CASTG 3 cut(s) 292, 369, 642
XapI RAATTY 1 cut(s) 491
XceI RCATGY 1 cut(s) 148
XcmI CCANNNNNNNNNTGG 1 cut(s) 457
XmiI GTMKAC 1 cut(s) 750
XspI CTAG 2 cut(s) 122, 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.