Rh3DG300900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0010631
Physical Location & Seq
Reverse (-)
31729679 .. 31730122
444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG300900.1

Sequence Viewer

Length: 444 bp
ATGTTGGCAGATCCAGCTGCGTTTGCTCTTGCCCAGGCGCAAGCCCAAGCCTATTTGATGCAGGTTGATCCGGTTTGTGAACCAGCCCTCATTCAAGTCTTGTTGGCCCAACAGGAAGCTGTTTTGGACCCTACTCTACTCGGGCCCAACCCGGACATGTCAATGTTTGATAATCCACTAGATCCATCTTCTCAATCAGTCCATTTTCAAGCACAGACTCCATCCCCGACCCTTCCTCCCTTCCTTTCATATCAAGCTCCGACTCCTTTGGTATCCAATTCTGCTTCTCCATTATCAGTTCTCCCGTCGGTTCCAGCTTTTCCTTTCTCTAGTGCTAGGGATCTTGTGCCAGGTTTCGATGCGGCCTCTCTTCCTCCTTTGGGTGCCCTTGCAATTCTGGCTCACCAATTCAAACAATTGCAACTCCGGAAGAGATTGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

147

Amino Acids

15.64

Weight (kDa)

4.51

Isoelectric Point (pI)

58.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 52
AccB1I GGYRCC 1 cut(s) 383
AccIII TCCGGA 1 cut(s) 426
AciI CCGC 1 cut(s) 362
AclWI GGATC 4 cut(s) 5, 62, 176, 348
AfiI CCNNNNNNNGG 1 cut(s) 380
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 3 cut(s) 95, 209, 412
AjnI CCWGG 2 cut(s) 33, 349
AluBI AGCT 4 cut(s) 17, 119, 257, 317
AluI AGCT 4 cut(s) 17, 119, 257, 317
AlwI GGATC 4 cut(s) 5, 62, 176, 348
Ama87I CYCGRG 1 cut(s) 140
Aor13HI TCCGGA 1 cut(s) 426
AoxI GGCC 3 cut(s) 105, 143, 363
ApaI GGGCCC 1 cut(s) 147
ApeKI GCWGC 1 cut(s) 17
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 4 cut(s) 106, 127, 143, 144
AsuC2I CCSGG 1 cut(s) 152
AsuHPI GGTGA 1 cut(s) 395
AvaI CYCGRG 1 cut(s) 140
AvaII GGWCC 1 cut(s) 127
BaeGI GKGCMC 2 cut(s) 147, 388
BanI GGYRCC 1 cut(s) 383
BanII GRGCYC 1 cut(s) 147
BbvI GCAGC 1 cut(s) 4
BccI CCATC 2 cut(s) 193, 229
BciT130I CCWGG 2 cut(s) 35, 351
BciVI GTATCC 1 cut(s) 283
BcnI CCSGG 1 cut(s) 152
BfaI CTAG 3 cut(s) 179, 330, 336
BfuAI ACCTGC 1 cut(s) 52
BfuI GTATCC 1 cut(s) 283
BisI GCNGC 2 cut(s) 18, 363
BlsI GCNGC 2 cut(s) 19, 364
Bme1390I CCNGG 3 cut(s) 35, 152, 351
Bme18I GGWCC 1 cut(s) 127
BmeT110I CYCGRG 1 cut(s) 140
BmgT120I GGNCC 4 cut(s) 106, 127, 143, 144
BmiI GGNNCC 4 cut(s) 129, 145, 312, 385
BmrFI CCNGG 3 cut(s) 35, 152, 351
BmsI GCATC 2 cut(s) 48, 349
BpuMI CCSGG 1 cut(s) 152
BsaJI CCNNGG 1 cut(s) 33
BsaWI WCCGGW 2 cut(s) 70, 426
BsaXI ACNNNNNCTCC 2 cut(s) 220, 250
Bsc4I CCNNNNNNNGG 1 cut(s) 380
BseAI TCCGGA 1 cut(s) 426
BseBI CCWGG 2 cut(s) 35, 351
BseDI CCNNGG 1 cut(s) 33
BseGI GGATG 1 cut(s) 221
BseLI CCNNNNNNNGG 1 cut(s) 380
BseSI GKGCMC 2 cut(s) 147, 388
BseXI GCAGC 1 cut(s) 4
BshFI GGCC 3 cut(s) 107, 145, 365
BshNI GGYRCC 1 cut(s) 383
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 3 cut(s) 71, 152, 427
BslI CCNNNNNNNGG 1 cut(s) 380
BsnI GGCC 3 cut(s) 107, 145, 365
BsoBI CYCGRG 1 cut(s) 140
Bsp120I GGGCCC 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 147, 388
Bsp13I TCCGGA 1 cut(s) 426
Bsp143I GATC 4 cut(s) 10, 67, 181, 340
BspACI CCGC 1 cut(s) 362
BspANI GGCC 3 cut(s) 107, 145, 365
BspEI TCCGGA 1 cut(s) 426
BspLI GGNNCC 4 cut(s) 129, 145, 312, 385
BspMI ACCTGC 1 cut(s) 52
BspPI GGATC 4 cut(s) 5, 62, 176, 348
BspT107I GGYRCC 1 cut(s) 383
BssECI CCNNGG 1 cut(s) 33
BssMI GATC 4 cut(s) 10, 67, 181, 340
Bst2UI CCWGG 2 cut(s) 35, 351
Bst6I CTCTTC 2 cut(s) 375, 425
BstC8I GCNNGC 1 cut(s) 42
BstF5I GGATG 1 cut(s) 221
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 4 cut(s) 13, 70, 184, 343
BstMBI GATC 4 cut(s) 10, 67, 181, 340
BstMWI GCNNNNNNNGC 3 cut(s) 14, 23, 398
BstNI CCWGG 2 cut(s) 35, 351
BstNSI RCATGY 1 cut(s) 160
BstSCI CCNGG 3 cut(s) 33, 150, 349
BstSLI GKGCMC 2 cut(s) 147, 388
BstV1I GCAGC 1 cut(s) 4
BstX2I RGATCY 3 cut(s) 10, 181, 340
BstYI RGATCY 3 cut(s) 10, 181, 340
BsuI GTATCC 1 cut(s) 283
BsuRI GGCC 3 cut(s) 107, 145, 365
BtsCI GGATG 1 cut(s) 221
BveI ACCTGC 1 cut(s) 52
Cac8I GCNNGC 1 cut(s) 42
CfoI GCGC 1 cut(s) 40
Cfr13I GGNCC 4 cut(s) 106, 127, 143, 144
CviAII CATG 1 cut(s) 157
DpnI GATC 4 cut(s) 12, 69, 183, 342
DpnII GATC 4 cut(s) 10, 67, 181, 340
Eam1104I CTCTTC 2 cut(s) 375, 425
EarI CTCTTC 2 cut(s) 375, 425
Eco24I GRGCYC 1 cut(s) 147
Eco47I GGWCC 1 cut(s) 127
Eco88I CYCGRG 1 cut(s) 140
EcoRII CCWGG 2 cut(s) 33, 349
EcoT38I GRGCYC 1 cut(s) 147
FaeI CATG 1 cut(s) 160
FaiI YATR 2 cut(s) 158, 250
FatI CATG 1 cut(s) 156
Fnu4HI GCNGC 2 cut(s) 18, 363
FokI GGATG 1 cut(s) 208
FriOI GRGCYC 1 cut(s) 147
Fsp4HI GCNGC 2 cut(s) 18, 363
FspBI CTAG 3 cut(s) 179, 330, 336
GlaI GCGC 1 cut(s) 39
GluI GCNGC 2 cut(s) 18, 363
HaeIII GGCC 3 cut(s) 107, 145, 365
HapII CCGG 3 cut(s) 71, 152, 427
HhaI GCGC 1 cut(s) 40
Hin1II CATG 1 cut(s) 160
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HinfI GANTC 2 cut(s) 217, 262
HpaII CCGG 3 cut(s) 71, 152, 427
HphI GGTGA 1 cut(s) 395
Hpy166II GTNNAC 1 cut(s) 80
Hpy188I TCNGA 1 cut(s) 261
Hpy188III TCNNGA 1 cut(s) 427
Hpy8I GTNNAC 1 cut(s) 80
Hpy99I CGWCG 1 cut(s) 310
HpyAV CCTTC 2 cut(s) 242, 250
HpyCH4V TGCA 4 cut(s) 61, 392, 421, 439
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 23, 398
Hsp92II CATG 1 cut(s) 160
HspAI GCGC 1 cut(s) 38
Kpn2I TCCGGA 1 cut(s) 426
Kzo9I GATC 4 cut(s) 10, 67, 181, 340
LmnI GCTCC 1 cut(s) 262
Lsp1109I GCAGC 1 cut(s) 4
LweI GCATC 2 cut(s) 48, 349
MaeI CTAG 3 cut(s) 179, 330, 336
MalI GATC 4 cut(s) 12, 69, 183, 342
MboI GATC 4 cut(s) 10, 67, 181, 340
MboII GAAGA 3 cut(s) 180, 362, 442
MfeI CAATTG 1 cut(s) 416
MflI RGATCY 3 cut(s) 10, 181, 340
MhlI GDGCHC 2 cut(s) 147, 388
MluCI AATT 4 cut(s) 277, 393, 407, 416
MlyI GAGTC 2 cut(s) 211, 256
MmeI TCCRAC 1 cut(s) 284
MnlI CCTC 4 cut(s) 98, 246, 376, 384
MroI TCCGGA 1 cut(s) 426
MslI CAYNNNNRTG 1 cut(s) 161
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 3 cut(s) 71, 152, 427
MspR9I CCNGG 3 cut(s) 35, 152, 351
MunI CAATTG 1 cut(s) 416
MvaI CCWGG 2 cut(s) 35, 351
MwoI GCNNNNNNNGC 3 cut(s) 14, 23, 398
NciI CCSGG 1 cut(s) 152
NdeII GATC 4 cut(s) 10, 67, 181, 340
NlaIII CATG 1 cut(s) 160
NlaIV GGNNCC 4 cut(s) 129, 145, 312, 385
NspI RCATGY 1 cut(s) 160
PciI ACATGT 1 cut(s) 156
PkrI GCNGC 2 cut(s) 19, 364
PleI GAGTC 2 cut(s) 211, 256
PpsI GAGTC 2 cut(s) 211, 256
PscI ACATGT 1 cut(s) 156
Psp6I CCWGG 2 cut(s) 33, 349
PspGI CCWGG 2 cut(s) 33, 349
PspN4I GGNNCC 4 cut(s) 129, 145, 312, 385
PspOMI GGGCCC 1 cut(s) 143
PspPI GGNCC 4 cut(s) 106, 127, 143, 144
PsuI RGATCY 3 cut(s) 10, 181, 340
PvuII CAGCTG 1 cut(s) 17
RseI CAYNNNNRTG 1 cut(s) 161
SatI GCNGC 2 cut(s) 18, 363
Sau3AI GATC 4 cut(s) 10, 67, 181, 340
Sau96I GGNCC 4 cut(s) 106, 127, 143, 144
SchI GAGTC 2 cut(s) 211, 256
ScrFI CCNGG 3 cut(s) 35, 152, 351
SduI GDGCHC 2 cut(s) 147, 388
SetI ASST 6 cut(s) 19, 66, 121, 259, 319, 355
SfaNI GCATC 2 cut(s) 48, 349
SinI GGWCC 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 161
Sse9I AATT 4 cut(s) 277, 393, 407, 416
SsiI CCGC 1 cut(s) 362
SspMI CTAG 3 cut(s) 179, 330, 336
StyD4I CCNGG 3 cut(s) 33, 150, 349
TaqI TCGA 1 cut(s) 357
TasI AATT 4 cut(s) 277, 393, 407, 416
TauI GCSGC 1 cut(s) 365
TseI GCWGC 1 cut(s) 17
TspDTI ATGAA 1 cut(s) 237
VpaK11BI GGWCC 1 cut(s) 127
XceI RCATGY 1 cut(s) 160
XspI CTAG 3 cut(s) 179, 330, 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.