Rh6DG295800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0010631
Physical Location & Seq
Reverse (-)
49684217 .. 49685545
1329 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG295800.1

Sequence Viewer

Length: 1329 bp
ATGCCTCGTCATCCGTCTGCCCATCACCTTGGGCCCTGGTTAGCGGTCCCTTCTCTCCGTAGGCCTCCCTGTGATCAAGACCATGGACTGGATATTGGTAATCCTCGTGGAATTAGATTACGACGGCCTTTGCCGGTTCCTGATCATGCTCCTGGTTTGCCAAATGGAAGGAACTTAGTTGTTCTTGCTCACGATCAGGTACGTGTTTTACCTACTGGCACACAGGTTGTTTTGGGTGTTCCAGGCGTTGCAGGTGACATTCAGGTTAATGTTATTCCCTGTGTCACGCCATCCTCTGCCCTTGACCTACTGGCAGGGGAAACTGGTGATGTCGGCTGCAATCCGCTGCCGGTTCCCGGAAACCTAGCCTTAGCTCATCGCTCACAAGGTCACTGGCAACTGCCCTCCACATCTCCTCCAATCACTACCTCCCAATCTGCATCGTCACCTCCCATGGGGCCCTCCTCAAGCAAATCTTTAGACAAGGGTAAACAAAAGATCTTTGCTGATGTGGTTCCATCTTCTTCCTCTCCACCTCATCAGCCTCTCAGTGATGTGGCGGACATGCAGTTAGCCTTATCACTCTACTTAGCCGATTTAGTTGAGTTCACTACTATGTTGACAGATCCAGCTGATGCCCAAGCCCATTTGATGCAAGTTGATCCGGTTTGTGAACCAACCCTCTTTCAAGTCTTGTTGGCCCATCAGGAAGCTCTTTTGGACCCTACTCTCCTCGGCCCCAACCTGGACATGTCAATGTTTGATCCTCCACCAGATCCATCTTCTCAATCACTCCCTTTTCAAGTACAGACTCCATCCTCGACCCTTCCTCCCTTCTCTTCATACCAAGCTCCGACTGCTTTGGTTTCCACTTCTGCTTCTCCATCATCAATTCTCCCGTCGGATTTAGATGCGGCCTCTCTTCCTCTTTTGGGTACTCTTGTGATTCTGGCTCACCAATTCAAACAATTGCAACTCTGGAAGAGATTGCATAAGGCCCTTGCTGATTCTTCGAATCCTTCCAAGAGGCATAAGGTGATAGCTGTTGTGGAAGATCCACCTTTTCCTCTGGTGTCTGCTCCACCTCCCACAAGACTTCGTCGATCCTGTGCTTCCCCTGGTCCAGGTCGCGGTCGTGGTCGTCGTGGCCGACCCCGAGATCGTGCTCTTAATCCACAGGTTACACATCCTGCCTCTCCTTTGGTTTTGGAATTTGAGCAGTCTAGCACTGTCGCAGTCACTCAGGATGATTATGTGGTTCCTCTTCCCCCCCCCCCCCCTTTGAGGACACTCCTGTTTCACTTCCTGCTTCTGGCACTCCATCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

442

Amino Acids

47.17

Weight (kDa)

6.78

Isoelectric Point (pI)

61.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 242
Acc36I ACCTGC 1 cut(s) 242
AccB7I CCANNNNNTGG 1 cut(s) 88
AccII CGCG 1 cut(s) 1131
AciI CCGC 5 cut(s) 44, 344, 560, 914, 1131
AclWI GGATC 6 cut(s) 620, 656, 758, 770, 1049, 1098
AcoI YGGCCR 1 cut(s) 1147
AcsI RAATTY 1 cut(s) 1211
AfaI GTAC 3 cut(s) 201, 807, 937
AfiI CCNNNNNNNGG 9 cut(s) 88, 356, 455, 745, 932, 1124, 1130, 1284, 1312
AflIII ACRYGT 2 cut(s) 202, 750
AgsI TTSAA 3 cut(s) 689, 803, 964
AjnI CCWGG 6 cut(s) 35, 151, 241, 744, 1117, 1123
AluBI AGCT 5 cut(s) 374, 632, 713, 851, 1043
AluI AGCT 5 cut(s) 374, 632, 713, 851, 1043
Alw21I GWGCWC 1 cut(s) 1168
AlwI GGATC 6 cut(s) 620, 656, 758, 770, 1049, 1098
Ama87I CYCGRG 1 cut(s) 1155
AoxI GGCC 9 cut(s) 32, 62, 125, 458, 699, 736, 915, 996, 1147
ApaI GGGCCC 2 cut(s) 36, 462
ApeKI GCWGC 2 cut(s) 336, 346
ApoI RAATTY 1 cut(s) 1211
AsuC2I CCSGG 1 cut(s) 357
AsuHPI GGTGA 6 cut(s) 17, 266, 338, 438, 947, 1048
AsuII TTCGAA 1 cut(s) 1013
AvaI CYCGRG 1 cut(s) 1155
AvaII GGWCC 3 cut(s) 46, 721, 1121
BaeGI GKGCMC 2 cut(s) 36, 462
BaeI ACNNNNGTAYC 2 cut(s) 927, 960
BanII GRGCYC 2 cut(s) 36, 462
BauI CACGAG 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 1168
BbvI GCAGC 2 cut(s) 323, 333
BccI CCATC 7 cut(s) 30, 298, 526, 711, 787, 823, 892
BceAI ACGGC 1 cut(s) 140
BciT130I CCWGG 6 cut(s) 37, 153, 243, 746, 1119, 1125
BclI TGATCA 2 cut(s) 73, 142
BcnI CCSGG 1 cut(s) 357
BfaI CTAG 3 cut(s) 365, 1224, 1327
BfuAI ACCTGC 1 cut(s) 242
BglII AGATCT 1 cut(s) 498
BisI GCNGC 3 cut(s) 337, 347, 915
BlsI GCNGC 3 cut(s) 338, 348, 916
Bme1390I CCNGG 7 cut(s) 37, 153, 243, 357, 746, 1119, 1125
Bme18I GGWCC 3 cut(s) 46, 721, 1121
BmeT110I CYCGRG 1 cut(s) 1155
BmrFI CCNGG 7 cut(s) 37, 153, 243, 357, 746, 1119, 1125
BmsI GCATC 4 cut(s) 449, 625, 642, 901
Bpu10I CCTNAGC 1 cut(s) 370
Bpu14I TTCGAA 1 cut(s) 1013
BpuEI CTTGAG 1 cut(s) 451
BpuMI CCSGG 1 cut(s) 357
BsaAI YACGTR 1 cut(s) 203
BsaJI CCNNGG 6 cut(s) 28, 35, 82, 453, 733, 1117
BsaWI WCCGGW 1 cut(s) 664
BsaXI ACNNNNNCTCC 4 cut(s) 400, 430, 814, 844
Bsc4I CCNNNNNNNGG 9 cut(s) 88, 356, 455, 745, 932, 1124, 1130, 1284, 1312
Bse118I RCCGGY 2 cut(s) 133, 349
Bse1I ACTGG 5 cut(s) 93, 220, 315, 328, 398
BseBI CCWGG 6 cut(s) 37, 153, 243, 746, 1119, 1125
BseDI CCNNGG 6 cut(s) 28, 35, 82, 453, 733, 1117
BseGI GGATG 5 cut(s) 10, 290, 815, 1186, 1252
BseLI CCNNNNNNNGG 9 cut(s) 88, 356, 455, 745, 932, 1124, 1130, 1284, 1312
BseMII CTCAG 2 cut(s) 562, 1256
BseNI ACTGG 5 cut(s) 93, 220, 315, 328, 398
BseRI GAGGAG 3 cut(s) 405, 454, 722
BseSI GKGCMC 2 cut(s) 36, 462
BseXI GCAGC 2 cut(s) 323, 333
Bsh1236I CGCG 1 cut(s) 1131
Bsh1285I CGRYCG 1 cut(s) 1135
BshFI GGCC 9 cut(s) 34, 64, 127, 460, 701, 738, 917, 998, 1149
BsiEI CGRYCG 1 cut(s) 1135
BsiHKAI GWGCWC 1 cut(s) 1168
BsiHKCI CYCGRG 1 cut(s) 1155
BsiSI CCGG 4 cut(s) 134, 350, 357, 665
BslFI GGGAC 1 cut(s) 32
BslI CCNNNNNNNGG 9 cut(s) 88, 356, 455, 745, 932, 1124, 1130, 1284, 1312
BsmFI GGGAC 1 cut(s) 32
BsnI GGCC 9 cut(s) 34, 64, 127, 460, 701, 738, 917, 998, 1149
BsoBI CYCGRG 1 cut(s) 1155
Bsp119I TTCGAA 1 cut(s) 1013
Bsp120I GGGCCC 2 cut(s) 32, 458
Bsp1286I GDGCHC 3 cut(s) 36, 462, 1168
Bsp19I CCATGG 2 cut(s) 82, 453
BspACI CCGC 5 cut(s) 44, 344, 560, 914, 1131
BspANI GGCC 9 cut(s) 34, 64, 127, 460, 701, 738, 917, 998, 1149
BspCNI CTCAG 2 cut(s) 561, 1255
BspFNI CGCG 1 cut(s) 1131
BspMI ACCTGC 1 cut(s) 242
BspPI GGATC 6 cut(s) 620, 656, 758, 770, 1049, 1098
BspT104I TTCGAA 1 cut(s) 1013
BsrFI RCCGGY 2 cut(s) 133, 349
BsrI ACTGG 5 cut(s) 93, 220, 315, 328, 398
BssAI RCCGGY 2 cut(s) 133, 349
BssECI CCNNGG 6 cut(s) 28, 35, 82, 453, 733, 1117
BssSI CACGAG 1 cut(s) 105
BssT1I CCWWGG 3 cut(s) 28, 82, 453
Bst2BI CACGAG 1 cut(s) 105
Bst2UI CCWGG 6 cut(s) 37, 153, 243, 746, 1119, 1125
Bst4CI ACNGT 1 cut(s) 1231
Bst6I CTCTTC 4 cut(s) 844, 927, 977, 1269
BstBAI YACGTR 1 cut(s) 203
BstBI TTCGAA 1 cut(s) 1013
BstDEI CTNAG 5 cut(s) 175, 370, 548, 589, 1242
BstDSI CCRYGG 2 cut(s) 82, 453
BstENI CCTNNNNNAGG 1 cut(s) 1122
BstF5I GGATG 5 cut(s) 10, 290, 815, 1186, 1252
BstFNI CGCG 1 cut(s) 1131
BstMCI CGRYCG 1 cut(s) 1135
BstMWI GCNNNNNNNGC 1 cut(s) 857
BstNI CCWGG 6 cut(s) 37, 153, 243, 746, 1119, 1125
BstNSI RCATGY 2 cut(s) 568, 754
BstSCI CCNGG 7 cut(s) 35, 151, 241, 355, 744, 1117, 1123
BstSLI GKGCMC 2 cut(s) 36, 462
BstUI CGCG 1 cut(s) 1131
BstV1I GCAGC 2 cut(s) 323, 333
BstX2I RGATCY 4 cut(s) 498, 625, 775, 1054
BstXI CCANNNNNNTGG 1 cut(s) 29
BstYI RGATCY 4 cut(s) 498, 625, 775, 1054
BsuRI GGCC 9 cut(s) 34, 64, 127, 460, 701, 738, 917, 998, 1149
BtgI CCRYGG 2 cut(s) 82, 453
BtgZI GCGATG 1 cut(s) 362
BtsCI GGATG 5 cut(s) 10, 290, 815, 1186, 1252
BtsIMutI CAGTG 3 cut(s) 391, 556, 1227
BveI ACCTGC 1 cut(s) 242
Cfr10I RCCGGY 2 cut(s) 133, 349
Csp6I GTAC 3 cut(s) 200, 806, 936
CviAII CATG 5 cut(s) 83, 146, 454, 565, 751
CviQI GTAC 3 cut(s) 200, 806, 936
DdeI CTNAG 5 cut(s) 175, 370, 548, 589, 1242
EaeI YGGCCR 1 cut(s) 1147
Eam1104I CTCTTC 4 cut(s) 844, 927, 977, 1269
EarI CTCTTC 4 cut(s) 844, 927, 977, 1269
EciI GGCGGA 1 cut(s) 575
Eco130I CCWWGG 3 cut(s) 28, 82, 453
Eco147I AGGCCT 1 cut(s) 64
Eco24I GRGCYC 2 cut(s) 36, 462
Eco47I GGWCC 3 cut(s) 46, 721, 1121
Eco88I CYCGRG 1 cut(s) 1155
EcoNI CCTNNNNNAGG 1 cut(s) 1122
EcoO109I RGGNCCY 4 cut(s) 33, 458, 459, 997
EcoRII CCWGG 6 cut(s) 35, 151, 241, 744, 1117, 1123
EcoT14I CCWWGG 3 cut(s) 28, 82, 453
EcoT38I GRGCYC 2 cut(s) 36, 462
ErhI CCWWGG 3 cut(s) 28, 82, 453
FaeI CATG 5 cut(s) 86, 149, 457, 568, 754
FalI AAGNNNNNCTT 2 cut(s) 460, 492
FaqI GGGAC 1 cut(s) 32
FatI CATG 5 cut(s) 82, 145, 453, 564, 750
FbaI TGATCA 2 cut(s) 73, 142
Fnu4HI GCNGC 3 cut(s) 337, 347, 915
FokI GGATG 4 cut(s) 277, 802, 1173, 1259
FriOI GRGCYC 2 cut(s) 36, 462
Fsp4HI GCNGC 3 cut(s) 337, 347, 915
FspBI CTAG 3 cut(s) 365, 1224, 1327
GluI GCNGC 3 cut(s) 337, 347, 915
HaeIII GGCC 9 cut(s) 34, 64, 127, 460, 701, 738, 917, 998, 1149
HapII CCGG 4 cut(s) 134, 350, 357, 665
Hin1II CATG 5 cut(s) 86, 149, 457, 568, 754
HincII GTYRAC 1 cut(s) 621
HindII GTYRAC 1 cut(s) 621
HinfI GANTC 4 cut(s) 811, 946, 1007, 1015
HpaII CCGG 4 cut(s) 134, 350, 357, 665
HphI GGTGA 6 cut(s) 17, 266, 338, 438, 947, 1048
Hpy166II GTNNAC 4 cut(s) 491, 609, 621, 674
Hpy188I TCNGA 2 cut(s) 855, 904
Hpy188III TCNNGA 6 cut(s) 77, 140, 191, 707, 979, 1244
Hpy8I GTNNAC 4 cut(s) 491, 609, 621, 674
Hpy99I CGWCG 4 cut(s) 126, 904, 1104, 1146
HpyAV CCTTC 5 cut(s) 60, 162, 836, 844, 1029
HpyCH4III ACNGT 1 cut(s) 1231
HpyCH4IV ACGT 1 cut(s) 202
HpyCH4V TGCA 7 cut(s) 251, 339, 440, 568, 655, 973, 991
HpyF10VI GCNNNNNNNGC 1 cut(s) 857
HpyF3I CTNAG 5 cut(s) 175, 370, 548, 589, 1242
HpySE526I ACGT 1 cut(s) 202
Hsp92II CATG 5 cut(s) 86, 149, 457, 568, 754
Ksp22I TGATCA 2 cut(s) 73, 142
LmnI GCTCC 3 cut(s) 154, 856, 1084
Lsp1109I GCAGC 2 cut(s) 323, 333
LweI GCATC 4 cut(s) 449, 625, 642, 901
MaeI CTAG 3 cut(s) 365, 1224, 1327
MaeII ACGT 1 cut(s) 202
MaeIII GTNAC 6 cut(s) 254, 283, 389, 444, 1180, 1237
MboII GAAGA 9 cut(s) 513, 516, 774, 831, 914, 994, 1002, 1064, 1256
MfeI CAATTG 1 cut(s) 968
MflI RGATCY 4 cut(s) 498, 625, 775, 1054
MhlI GDGCHC 3 cut(s) 36, 462, 1168
MluCI AATT 5 cut(s) 111, 891, 959, 968, 1211
MlyI GAGTC 1 cut(s) 805
MmeI TCCRAC 2 cut(s) 878, 882
MseI TTAA 2 cut(s) 267, 1170
MslI CAYNNNNRTG 2 cut(s) 614, 755
MspA1I CMGCKG 2 cut(s) 346, 632
MspI CCGG 4 cut(s) 134, 350, 357, 665
MspR9I CCNGG 7 cut(s) 37, 153, 243, 357, 746, 1119, 1125
MunI CAATTG 1 cut(s) 968
MvaI CCWGG 6 cut(s) 37, 153, 243, 746, 1119, 1125
MvnI CGCG 1 cut(s) 1131
MwoI GCNNNNNNNGC 1 cut(s) 857
NciI CCSGG 1 cut(s) 357
NcoI CCATGG 2 cut(s) 82, 453
NlaIII CATG 5 cut(s) 86, 149, 457, 568, 754
NmeAIII GCCGAG 1 cut(s) 714
NmuCI GTSAC 5 cut(s) 254, 283, 389, 444, 1237
NspI RCATGY 2 cut(s) 568, 754
NspV TTCGAA 1 cut(s) 1013
PaqCI CACCTGC 1 cut(s) 242
PceI AGGCCT 1 cut(s) 64
PciI ACATGT 1 cut(s) 750
PcsI WCGNNNNNNNCGW 2 cut(s) 1141, 1147
PfeI GAWTC 3 cut(s) 946, 1007, 1015
PflFI GACNNNGTC 1 cut(s) 1098
PflMI CCANNNNNTGG 1 cut(s) 88
PfoI TCCNGGA 1 cut(s) 355
PkrI GCNGC 3 cut(s) 338, 348, 916
PleI GAGTC 1 cut(s) 805
PpsI GAGTC 1 cut(s) 805
Ppu21I YACGTR 1 cut(s) 203
PscI ACATGT 1 cut(s) 750
Psp6I CCWGG 6 cut(s) 35, 151, 241, 744, 1117, 1123
PspGI CCWGG 6 cut(s) 35, 151, 241, 744, 1117, 1123
PspOMI GGGCCC 2 cut(s) 32, 458
PsuI RGATCY 4 cut(s) 498, 625, 775, 1054
PsyI GACNNNGTC 1 cut(s) 1098
PvuII CAGCTG 1 cut(s) 632
RsaI GTAC 3 cut(s) 201, 807, 937
RsaNI GTAC 3 cut(s) 200, 806, 936
RseI CAYNNNNRTG 2 cut(s) 614, 755
SaqAI TTAA 2 cut(s) 267, 1170
SatI GCNGC 3 cut(s) 337, 347, 915
SchI GAGTC 1 cut(s) 805
ScrFI CCNGG 7 cut(s) 37, 153, 243, 357, 746, 1119, 1125
SduI GDGCHC 3 cut(s) 36, 462, 1168
SfaNI GCATC 4 cut(s) 449, 625, 642, 901
SfuI TTCGAA 1 cut(s) 1013
SinI GGWCC 3 cut(s) 46, 721, 1121
SmiMI CAYNNNNRTG 2 cut(s) 614, 755
SmlI CTYRAG 1 cut(s) 466
SmoI CTYRAG 1 cut(s) 466
Sse9I AATT 5 cut(s) 111, 891, 959, 968, 1211
SseBI AGGCCT 1 cut(s) 64
SsiI CCGC 5 cut(s) 44, 344, 560, 914, 1131
SspMI CTAG 3 cut(s) 365, 1224, 1327
StuI AGGCCT 1 cut(s) 64
StyD4I CCNGG 7 cut(s) 35, 151, 241, 355, 744, 1117, 1123
StyI CCWWGG 3 cut(s) 28, 82, 453
TaaI ACNGT 1 cut(s) 1231
TaiI ACGT 1 cut(s) 205
TaqI TCGA 3 cut(s) 821, 1013, 1102
TasI AATT 5 cut(s) 111, 891, 959, 968, 1211
TatI WGTACW 1 cut(s) 805
TauI GCSGC 1 cut(s) 917
TfiI GAWTC 3 cut(s) 946, 1007, 1015
Tru1I TTAA 2 cut(s) 267, 1170
Tru9I TTAA 2 cut(s) 267, 1170
TscAI CASTG 3 cut(s) 398, 556, 1234
TseFI GTSAC 5 cut(s) 254, 283, 389, 444, 1237
TseI GCWGC 2 cut(s) 336, 346
Tsp45I GTSAC 5 cut(s) 254, 283, 389, 444, 1237
TspDTI ATGAA 1 cut(s) 831
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 3 cut(s) 398, 556, 1234
Tth111I GACNNNGTC 1 cut(s) 1098
Van91I CCANNNNNTGG 1 cut(s) 88
VpaK11BI GGWCC 3 cut(s) 46, 721, 1121
XagI CCTNNNNNAGG 1 cut(s) 1122
XapI RAATTY 1 cut(s) 1211
XceI RCATGY 2 cut(s) 568, 754
XspI CTAG 3 cut(s) 365, 1224, 1327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.