Rh6BG256300
HSP70 Family

Heat shock cognate 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0010631
Physical Location & Seq
Reverse (-)
46959015 .. 46960169
1155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG256300.1

Sequence Viewer

Length: 993 bp
ATGGCATGGCTTACGCGTGGCAGGCGACTCCGATCCCGTTTGCCTTTAAAAGGTTTCATGCATGCCAACACTCCGTACTCCAAACCTACTTCACAATCACATCCCATTCTCATGGCAGACGCCCCTCGCTCCCCACATTCTCCCCCAGCTGATGAGCAGCTTGGAGAGCTCTTTCTCCATTTACGTCCGCCAGAACCCGACCCTGCTGATGGTACCCAGCTGGTTGGGTATCTCATCTCTGATCGTAGTCATAACCCACAGGCGGTCATCCGTATCCTTACACAAGCCTGGGCTGCGATAGGTCGTTTCCACTTGCAACTTATCAAGGGCAACGATGTCTTCTCTATTGCTGCTTCCTCAGCTGCTGCTGCTGACCAGCTTCTTGAGCGCAGACCCTGGGTTCTTGAGGTGGATGATCCTCAGGTCCCTATTTCCTGGATTGGTTTTCTCTGTATTAGGGTTCAACTGGACTCTCGTGCTCCCCTCGCTCCAGGCTTTTGGTTTGATGTGTCAAGAGGGGACCCGGTTTGGGCAGATTTTTGCTATGAACGACTTTCGGAGATGTGTTTTCTTTGTGGTCGTCTTGGCCATATGATTTCACGGTGTCAGATGCCTCGTCATCCGTCTGCCCATCACCTTGGGCCCTGGTTAGCGGTCCCTTTTCTCCGCAGGCCTCCTTATGATCAAGACCATGGACTGGATATTGGTAATCCTCGTGGAATTGGATTACGACGGCCTTTGCCAGTTCCTGATCATGATCCTAGTTCGCCAAATGGAAGGAACTTAGTTGTTCTTACTCATGATCAGGTATGTGTTTTACCTACTGGCACACAGGTTGTTCTGGGTTTTCCAGGTGTTGCAGGTGACATTCAGGTTAATGTTATTCCCTATGTCACGCCATCCCCTGCCCTTGACCTACCTCCACAAACTGGTGATGTCGGCTGCAATCTGCTGCCGGTTCCAGGAAACCTAGTATTAGCACATCGCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

330

Amino Acids

36.36

Weight (kDa)

7.23

Isoelectric Point (pI)

53.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 158 - 203 2.8e-09 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 851
Acc36I ACCTGC 1 cut(s) 851
Acc65I GGTACC 1 cut(s) 212
AccB1I GGYRCC 1 cut(s) 212
AccB7I CCANNNNNTGG 2 cut(s) 697, 929
AccII CGCG 1 cut(s) 16
AciI CCGC 4 cut(s) 188, 263, 653, 667
AclWI GGATC 3 cut(s) 27, 410, 752
AcoI YGGCCR 1 cut(s) 586
AcyI GRCGYC 1 cut(s) 120
AfaI GTAC 2 cut(s) 77, 214
AfiI CCNNNNNNNGG 7 cut(s) 50, 209, 262, 529, 697, 929, 962
AflIII ACRYGT 1 cut(s) 14
AgsI TTSAA 1 cut(s) 464
AjnI CCWGG 7 cut(s) 287, 395, 434, 490, 644, 850, 961
AluBI AGCT 6 cut(s) 149, 160, 169, 220, 362, 379
AluI AGCT 6 cut(s) 149, 160, 169, 220, 362, 379
Alw21I GWGCWC 2 cut(s) 171, 481
AlwI GGATC 3 cut(s) 27, 410, 752
AlwNI CAGNNNCTG 3 cut(s) 365, 396, 749
AoxI GGCC 4 cut(s) 586, 641, 671, 734
ApaI GGGCCC 1 cut(s) 645
ApeKI GCWGC 8 cut(s) 157, 293, 350, 362, 365, 368, 942, 952
Asp718I GGTACC 1 cut(s) 212
AspLEI GCGC 1 cut(s) 390
AspS9I GGNCC 5 cut(s) 424, 520, 641, 642, 655
AsuC2I CCSGG 1 cut(s) 524
AsuHPI GGTGA 3 cut(s) 626, 875, 944
AvaII GGWCC 3 cut(s) 424, 520, 655
AxyI CCTNAGG 1 cut(s) 420
BaeGI GKGCMC 1 cut(s) 645
BalI TGGCCA 1 cut(s) 588
BanI GGYRCC 1 cut(s) 212
BanII GRGCYC 2 cut(s) 171, 645
BauI CACGAG 2 cut(s) 474, 714
BbsI GAAGAC 1 cut(s) 331
Bbv12I GWGCWC 2 cut(s) 171, 481
BbvCI CCTCAGC 1 cut(s) 358
BbvI GCAGC 8 cut(s) 169, 280, 337, 349, 352, 355, 929, 939
BccI CCATC 3 cut(s) 203, 639, 907
BceAI ACGGC 1 cut(s) 749
BciT130I CCWGG 7 cut(s) 289, 397, 436, 492, 646, 852, 963
BciVI GTATCC 1 cut(s) 284
BclI TGATCA 3 cut(s) 682, 751, 802
BcnI CCSGG 1 cut(s) 524
BfaI CTAG 2 cut(s) 762, 971
BfuAI ACCTGC 1 cut(s) 851
BfuI GTATCC 1 cut(s) 284
BisI GCNGC 8 cut(s) 158, 294, 351, 363, 366, 369, 943, 953
BlsI GCNGC 8 cut(s) 159, 295, 352, 364, 367, 370, 944, 954
Bme1390I CCNGG 8 cut(s) 289, 397, 436, 492, 524, 646, 852, 963
Bme18I GGWCC 3 cut(s) 424, 520, 655
BmgT120I GGNCC 5 cut(s) 424, 520, 641, 642, 655
BmiI GGNNCC 7 cut(s) 214, 426, 521, 522, 643, 657, 960
BmrFI CCNGG 8 cut(s) 289, 397, 436, 492, 524, 646, 852, 963
BmsI GCATC 1 cut(s) 600
BpiI GAAGAC 1 cut(s) 331
BpmI CTGGAG 1 cut(s) 474
Bpu10I CCTNAGC 1 cut(s) 358
BpuEI CTTGAG 2 cut(s) 404, 425
BpuMI CCSGG 1 cut(s) 524
BsaBI GATNNNNATC 1 cut(s) 756
BsaHI GRCGYC 1 cut(s) 120
BsaJI CCNNGG 6 cut(s) 288, 395, 396, 637, 644, 691
Bsc4I CCNNNNNNNGG 7 cut(s) 50, 209, 262, 529, 697, 929, 962
Bse118I RCCGGY 1 cut(s) 955
Bse1I ACTGG 5 cut(s) 471, 702, 743, 829, 934
Bse21I CCTNAGG 1 cut(s) 420
Bse8I GATNNNNATC 1 cut(s) 756
BseBI CCWGG 7 cut(s) 289, 397, 436, 492, 646, 852, 963
BseDI CCNNGG 6 cut(s) 288, 395, 396, 637, 644, 691
BseGI GGATG 5 cut(s) 100, 267, 418, 619, 899
BseJI GATNNNNATC 1 cut(s) 756
BseLI CCNNNNNNNGG 7 cut(s) 50, 209, 262, 529, 697, 929, 962
BseMII CTCAG 2 cut(s) 372, 434
BseNI ACTGG 5 cut(s) 471, 702, 743, 829, 934
BseSI GKGCMC 1 cut(s) 645
BseXI GCAGC 8 cut(s) 169, 280, 337, 349, 352, 355, 929, 939
BseYI CCCAGC 2 cut(s) 145, 216
Bsh1236I CGCG 1 cut(s) 16
BshFI GGCC 4 cut(s) 588, 643, 673, 736
BshNI GGYRCC 1 cut(s) 212
BsiHKAI GWGCWC 2 cut(s) 171, 481
BsiSI CCGG 2 cut(s) 524, 956
BslFI GGGAC 3 cut(s) 410, 533, 641
BslI CCNNNNNNNGG 7 cut(s) 50, 209, 262, 529, 697, 929, 962
BsmFI GGGAC 3 cut(s) 410, 533, 641
BsnI GGCC 4 cut(s) 588, 643, 673, 736
Bsp120I GGGCCC 1 cut(s) 641
Bsp1286I GDGCHC 3 cut(s) 171, 481, 645
Bsp143I GATC 7 cut(s) 32, 241, 415, 682, 751, 757, 802
Bsp19I CCATGG 1 cut(s) 691
BspACI CCGC 4 cut(s) 188, 263, 653, 667
BspANI GGCC 4 cut(s) 588, 643, 673, 736
BspCNI CTCAG 2 cut(s) 371, 433
BspFNI CGCG 1 cut(s) 16
BspHI TCATGA 2 cut(s) 754, 799
BspLI GGNNCC 7 cut(s) 214, 426, 521, 522, 643, 657, 960
BspMI ACCTGC 1 cut(s) 851
BspPI GGATC 3 cut(s) 27, 410, 752
BspT107I GGYRCC 1 cut(s) 212
BsrFI RCCGGY 1 cut(s) 955
BsrI ACTGG 5 cut(s) 471, 702, 743, 829, 934
BssAI RCCGGY 1 cut(s) 955
BssECI CCNNGG 6 cut(s) 288, 395, 396, 637, 644, 691
BssMI GATC 7 cut(s) 32, 241, 415, 682, 751, 757, 802
BssNI GRCGYC 1 cut(s) 120
BssSI CACGAG 2 cut(s) 474, 714
BssT1I CCWWGG 2 cut(s) 637, 691
Bst2BI CACGAG 2 cut(s) 474, 714
Bst2UI CCWGG 7 cut(s) 289, 397, 436, 492, 646, 852, 963
Bst4CI ACNGT 1 cut(s) 603
BstACI GRCGYC 1 cut(s) 120
BstC8I GCNNGC 3 cut(s) 23, 63, 671
BstDEI CTNAG 3 cut(s) 358, 420, 784
BstDSI CCRYGG 1 cut(s) 691
BstENI CCTNNNNNAGG 1 cut(s) 48
BstF5I GGATG 5 cut(s) 100, 267, 418, 619, 899
BstFNI CGCG 1 cut(s) 16
BstHHI GCGC 1 cut(s) 390
BstKTI GATC 7 cut(s) 35, 244, 418, 685, 754, 760, 805
BstMBI GATC 7 cut(s) 32, 241, 415, 682, 751, 757, 802
BstMWI GCNNNNNNNGC 7 cut(s) 22, 166, 293, 359, 368, 385, 485
BstNI CCWGG 7 cut(s) 289, 397, 436, 492, 646, 852, 963
BstNSI RCATGY 1 cut(s) 65
BstSCI CCNGG 8 cut(s) 287, 395, 434, 490, 522, 644, 850, 961
BstSLI GKGCMC 1 cut(s) 645
BstUI CGCG 1 cut(s) 16
BstV1I GCAGC 8 cut(s) 169, 280, 337, 349, 352, 355, 929, 939
BstV2I GAAGAC 1 cut(s) 331
BstXI CCANNNNNNTGG 4 cut(s) 112, 224, 498, 638
Bsu36I CCTNAGG 1 cut(s) 420
BsuI GTATCC 1 cut(s) 284
BsuRI GGCC 4 cut(s) 588, 643, 673, 736
BtgI CCRYGG 1 cut(s) 691
BtgZI GCGATG 1 cut(s) 968
BtsCI GGATG 5 cut(s) 100, 267, 418, 619, 899
BveI ACCTGC 1 cut(s) 851
Cac8I GCNNGC 3 cut(s) 23, 63, 671
CaiI CAGNNNCTG 3 cut(s) 365, 396, 749
CciI TCATGA 2 cut(s) 754, 799
CfoI GCGC 1 cut(s) 390
Cfr10I RCCGGY 1 cut(s) 955
Cfr13I GGNCC 5 cut(s) 424, 520, 641, 642, 655
CseI GACGC 1 cut(s) 128
Csp6I GTAC 2 cut(s) 76, 213
CviAII CATG 7 cut(s) 6, 58, 62, 112, 692, 755, 800
CviQI GTAC 2 cut(s) 76, 213
DdeI CTNAG 3 cut(s) 358, 420, 784
DpnI GATC 7 cut(s) 34, 243, 417, 684, 753, 759, 804
DpnII GATC 7 cut(s) 32, 241, 415, 682, 751, 757, 802
DraI TTTAAA 1 cut(s) 48
EaeI YGGCCR 1 cut(s) 586
EciI GGCGGA 1 cut(s) 177
Ecl136II GAGCTC 1 cut(s) 169
Eco130I CCWWGG 2 cut(s) 637, 691
Eco147I AGGCCT 1 cut(s) 673
Eco24I GRGCYC 2 cut(s) 171, 645
Eco47I GGWCC 3 cut(s) 424, 520, 655
Eco53kI GAGCTC 1 cut(s) 169
Eco81I CCTNAGG 1 cut(s) 420
EcoICRI GAGCTC 1 cut(s) 169
EcoNI CCTNNNNNAGG 1 cut(s) 48
EcoO109I RGGNCCY 3 cut(s) 424, 520, 642
EcoRII CCWGG 7 cut(s) 287, 395, 434, 490, 644, 850, 961
EcoT14I CCWWGG 2 cut(s) 637, 691
EcoT22I ATGCAT 1 cut(s) 63
EcoT38I GRGCYC 2 cut(s) 171, 645
ErhI CCWWGG 2 cut(s) 637, 691
FaeI CATG 7 cut(s) 9, 61, 65, 115, 695, 758, 803
FaqI GGGAC 3 cut(s) 410, 533, 641
FatI CATG 7 cut(s) 5, 57, 61, 111, 691, 754, 799
FauNDI CATATG 1 cut(s) 591
FbaI TGATCA 3 cut(s) 682, 751, 802
Fnu4HI GCNGC 8 cut(s) 158, 294, 351, 363, 366, 369, 943, 953
FokI GGATG 5 cut(s) 87, 254, 425, 606, 886
FriOI GRGCYC 2 cut(s) 171, 645
Fsp4HI GCNGC 8 cut(s) 158, 294, 351, 363, 366, 369, 943, 953
FspBI CTAG 2 cut(s) 762, 971
GlaI GCGC 1 cut(s) 389
GluI GCNGC 8 cut(s) 158, 294, 351, 363, 366, 369, 943, 953
GsaI CCCAGC 2 cut(s) 149, 220
GsuI CTGGAG 1 cut(s) 474
HaeIII GGCC 4 cut(s) 588, 643, 673, 736
HapII CCGG 2 cut(s) 524, 956
HgaI GACGC 1 cut(s) 128
HhaI GCGC 1 cut(s) 390
Hin1I GRCGYC 1 cut(s) 120
Hin1II CATG 7 cut(s) 9, 61, 65, 115, 695, 758, 803
Hin6I GCGC 1 cut(s) 388
HinP1I GCGC 1 cut(s) 388
HinfI GANTC 2 cut(s) 27, 470
HpaII CCGG 2 cut(s) 524, 956
HphI GGTGA 3 cut(s) 626, 875, 944
Hpy188I TCNGA 4 cut(s) 32, 241, 559, 609
Hpy188III TCNNGA 7 cut(s) 383, 404, 513, 686, 749, 755, 800
Hpy99I CGWCG 1 cut(s) 735
HpyAV CCTTC 1 cut(s) 771
HpyCH4III ACNGT 1 cut(s) 603
HpyCH4IV ACGT 1 cut(s) 184
HpyCH4V TGCA 4 cut(s) 61, 316, 860, 945
HpyF10VI GCNNNNNNNGC 7 cut(s) 22, 166, 293, 359, 368, 385, 485
HpyF3I CTNAG 3 cut(s) 358, 420, 784
HpySE526I ACGT 1 cut(s) 184
Hsp92I GRCGYC 1 cut(s) 120
Hsp92II CATG 7 cut(s) 9, 61, 65, 115, 695, 758, 803
HspAI GCGC 1 cut(s) 388
KflI GGGWCCC 1 cut(s) 520
KpnI GGTACC 1 cut(s) 216
Ksp22I TGATCA 3 cut(s) 682, 751, 802
Kzo9I GATC 7 cut(s) 32, 241, 415, 682, 751, 757, 802
LmnI GCTCC 3 cut(s) 134, 484, 493
Lsp1109I GCAGC 8 cut(s) 169, 280, 337, 349, 352, 355, 929, 939
LweI GCATC 1 cut(s) 600
MaeI CTAG 2 cut(s) 762, 971
MaeII ACGT 1 cut(s) 184
MaeIII GTNAC 2 cut(s) 863, 892
MalI GATC 7 cut(s) 34, 243, 417, 684, 753, 759, 804
MboI GATC 7 cut(s) 32, 241, 415, 682, 751, 757, 802
MboII GAAGA 1 cut(s) 331
MhlI GDGCHC 3 cut(s) 171, 481, 645
MlsI TGGCCA 1 cut(s) 588
MluCI AATT 1 cut(s) 720
MluI ACGCGT 1 cut(s) 14
MluNI TGGCCA 1 cut(s) 588
MlyI GAGTC 2 cut(s) 21, 464
Mox20I TGGCCA 1 cut(s) 588
Mph1103I ATGCAT 1 cut(s) 63
MscI TGGCCA 1 cut(s) 588
MseI TTAA 2 cut(s) 47, 876
MslI CAYNNNNRTG 1 cut(s) 110
Msp20I TGGCCA 1 cut(s) 588
MspA1I CMGCKG 3 cut(s) 149, 220, 362
MspI CCGG 2 cut(s) 524, 956
MspR9I CCNGG 8 cut(s) 289, 397, 436, 492, 524, 646, 852, 963
MvaI CCWGG 7 cut(s) 289, 397, 436, 492, 646, 852, 963
MvnI CGCG 1 cut(s) 16
MwoI GCNNNNNNNGC 7 cut(s) 22, 166, 293, 359, 368, 385, 485
NciI CCSGG 1 cut(s) 524
NcoI CCATGG 1 cut(s) 691
NdeI CATATG 1 cut(s) 591
NdeII GATC 7 cut(s) 32, 241, 415, 682, 751, 757, 802
NlaIII CATG 7 cut(s) 9, 61, 65, 115, 695, 758, 803
NlaIV GGNNCC 7 cut(s) 214, 426, 521, 522, 643, 657, 960
NmuCI GTSAC 2 cut(s) 863, 892
NsiI ATGCAT 1 cut(s) 63
NspI RCATGY 1 cut(s) 65
PaeI GCATGC 1 cut(s) 65
PagI TCATGA 2 cut(s) 754, 799
PaqCI CACCTGC 1 cut(s) 851
PasI CCCWGGG 1 cut(s) 396
PceI AGGCCT 1 cut(s) 673
PflMI CCANNNNNTGG 2 cut(s) 697, 929
PfoI TCCNGGA 2 cut(s) 434, 961
PkrI GCNGC 8 cut(s) 159, 295, 352, 364, 367, 370, 944, 954
PleI GAGTC 2 cut(s) 21, 464
PpsI GAGTC 2 cut(s) 21, 464
PpuMI RGGWCCY 2 cut(s) 424, 520
Psp124BI GAGCTC 1 cut(s) 171
Psp5II RGGWCCY 2 cut(s) 424, 520
Psp6I CCWGG 7 cut(s) 287, 395, 434, 490, 644, 850, 961
PspFI CCCAGC 2 cut(s) 145, 216
PspGI CCWGG 7 cut(s) 287, 395, 434, 490, 644, 850, 961
PspN4I GGNNCC 7 cut(s) 214, 426, 521, 522, 643, 657, 960
PspOMI GGGCCC 1 cut(s) 641
PspPI GGNCC 5 cut(s) 424, 520, 641, 642, 655
PspPPI RGGWCCY 2 cut(s) 424, 520
PstNI CAGNNNCTG 3 cut(s) 365, 396, 749
PvuII CAGCTG 3 cut(s) 149, 220, 362
RsaI GTAC 2 cut(s) 77, 214
RsaNI GTAC 2 cut(s) 76, 213
RseI CAYNNNNRTG 1 cut(s) 110
SacI GAGCTC 1 cut(s) 171
SaqAI TTAA 2 cut(s) 47, 876
SatI GCNGC 8 cut(s) 158, 294, 351, 363, 366, 369, 943, 953
Sau3AI GATC 7 cut(s) 32, 241, 415, 682, 751, 757, 802
Sau96I GGNCC 5 cut(s) 424, 520, 641, 642, 655
SchI GAGTC 2 cut(s) 21, 464
ScrFI CCNGG 8 cut(s) 289, 397, 436, 492, 524, 646, 852, 963
SduI GDGCHC 3 cut(s) 171, 481, 645
SfaNI GCATC 1 cut(s) 600
SinI GGWCC 3 cut(s) 424, 520, 655
SmiMI CAYNNNNRTG 1 cut(s) 110
SmlI CTYRAG 2 cut(s) 383, 404
SmoI CTYRAG 2 cut(s) 383, 404
SphI GCATGC 1 cut(s) 65
Sse9I AATT 1 cut(s) 720
SseBI AGGCCT 1 cut(s) 673
SsiI CCGC 4 cut(s) 188, 263, 653, 667
SspMI CTAG 2 cut(s) 762, 971
SstI GAGCTC 1 cut(s) 171
StuI AGGCCT 1 cut(s) 673
StyD4I CCNGG 8 cut(s) 287, 395, 434, 490, 522, 644, 850, 961
StyI CCWWGG 2 cut(s) 637, 691
TaaI ACNGT 1 cut(s) 603
TaiI ACGT 1 cut(s) 187
TasI AATT 1 cut(s) 720
Tru1I TTAA 2 cut(s) 47, 876
Tru9I TTAA 2 cut(s) 47, 876
TseFI GTSAC 2 cut(s) 863, 892
TseI GCWGC 8 cut(s) 157, 293, 350, 362, 365, 368, 942, 952
Tsp45I GTSAC 2 cut(s) 863, 892
TspDTI ATGAA 2 cut(s) 46, 561
TspGWI ACGGA 3 cut(s) 63, 260, 612
Van91I CCANNNNNTGG 2 cut(s) 697, 929
VpaK11BI GGWCC 3 cut(s) 424, 520, 655
XagI CCTNNNNNAGG 1 cut(s) 48
XceI RCATGY 1 cut(s) 65
XspI CTAG 2 cut(s) 762, 971
Zsp2I ATGCAT 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.