Rh5AG196600

two-component response regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0005174
Physical Location & Seq
Forward (+)
22963370 .. 22967763
4394 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG196600.1

Sequence Viewer

Length: 618 bp
ATGGAGGTAGTGAAGTCATCAGAATTAGTTACAGCAGACTCCAAACAACAGCAACAGTTCCATCTGTTGCTTGCGGATGACAGTGAGCTGGACAGGAAACTCGGAGAGACGCTCCTTAAACGTTCTACCTTCAAAGCATTGACCTGTGTGGATTCTGGGGATGAAGCTCTTAAGACTTTAGGTTTGCTTGATATCGATCTAAACCAAAGTTCTTCGGATTCTACTCCAACCATTTCGCCGAATTCATCATCAGATCAAGAGGGACCATTCAATTTTATCATCACAGATTACTGTATGCCAGGGATGAGCGGTTATGATTTGCTGAAACGGATCAAGGGATCTTACTGGAAAGATGTACCAGTCGTGCTTATGTCAGCAGATAATGTACCTTCAAGGATTAGCATGTGCTTGGAAGAAGGGGCTGAGGCGTTTCCTTTGAAGCCACCAAAGTTATCAGATTTGAGAAAGCTAATTCAGCCTTACCTGATCAAATCACTCCACCATTCCGGTAACAACAACACTGACAGTAGTTTGGAGGTAACCAACAGGAAGAGGCGCTACAACTTCATTCAAGTCCGCCGGCTGTCATCCCTGGACTTGATCGCTGAGGAGAGCTAA

Protein Analysis

205

Amino Acids

22.9

Weight (kDa)

5.28

Isoelectric Point (pI)

53.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Response_reg PF00072 87 - 158 7.3e-12 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 309
AciI CCGC 3 cut(s) 74, 309, 577
AclI AACGTT 1 cut(s) 121
AclWI GGATC 2 cut(s) 338, 346
AcsI RAATTY 1 cut(s) 241
AfaI GTAC 2 cut(s) 357, 387
AflII CTTAAG 1 cut(s) 170
AgsI TTSAA 5 cut(s) 133, 271, 393, 439, 572
AjnI CCWGG 2 cut(s) 298, 591
AluBI AGCT 4 cut(s) 88, 167, 469, 615
AluI AGCT 4 cut(s) 88, 167, 469, 615
Alw26I GTCTC 1 cut(s) 101
AlwI GGATC 2 cut(s) 338, 346
ApoI RAATTY 1 cut(s) 241
ArsI GACNNNNNNTTYG 2 cut(s) 166, 198
AspLEI GCGC 1 cut(s) 558
AspS9I GGNCC 1 cut(s) 263
AvaII GGWCC 1 cut(s) 263
BarI GAAGNNNNNNTAC 2 cut(s) 542, 574
BbvCI CCTCAGC 2 cut(s) 423, 606
BccI CCATC 1 cut(s) 69
BciT130I CCWGG 2 cut(s) 300, 593
BclI TGATCA 1 cut(s) 486
BcoDI GTCTC 1 cut(s) 101
BfoI RGCGCY 1 cut(s) 559
BfrI CTTAAG 1 cut(s) 170
Bme1390I CCNGG 2 cut(s) 300, 593
Bme18I GGWCC 1 cut(s) 263
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 1 cut(s) 264
BmrFI CCNGG 2 cut(s) 300, 593
BplI GAGNNNNNCTC 2 cut(s) 96, 128
Bpu10I CCTNAGC 2 cut(s) 423, 606
Bsa29I ATCGAT 1 cut(s) 195
BsaBI GATNNNNATC 1 cut(s) 195
BsaJI CCNNGG 2 cut(s) 299, 591
BsaWI WCCGGW 1 cut(s) 506
Bse118I RCCGGY 1 cut(s) 579
Bse1I ACTGG 2 cut(s) 350, 359
Bse8I GATNNNNATC 1 cut(s) 195
BseBI CCWGG 2 cut(s) 300, 593
BseCI ATCGAT 1 cut(s) 195
BseDI CCNNGG 2 cut(s) 299, 591
BseGI GGATG 4 cut(s) 82, 166, 309, 587
BseJI GATNNNNATC 1 cut(s) 195
BseMII CTCAG 2 cut(s) 414, 597
BseNI ACTGG 2 cut(s) 350, 359
BshVI ATCGAT 1 cut(s) 195
BsiSI CCGG 2 cut(s) 507, 580
BslFI GGGAC 1 cut(s) 276
BsmAI GTCTC 1 cut(s) 101
BsmBI CGTCTC 1 cut(s) 101
BsmFI GGGAC 1 cut(s) 276
Bsp143I GATC 6 cut(s) 196, 253, 330, 338, 486, 600
BspACI CCGC 3 cut(s) 74, 309, 577
BspCNI CTCAG 2 cut(s) 415, 598
BspDI ATCGAT 1 cut(s) 195
BspLI GGNNCC 1 cut(s) 264
BspPI GGATC 2 cut(s) 338, 346
BspTI CTTAAG 1 cut(s) 170
BsrBI CCGCTC 1 cut(s) 309
BsrFI RCCGGY 1 cut(s) 579
BsrI ACTGG 2 cut(s) 350, 359
BssAI RCCGGY 1 cut(s) 579
BssECI CCNNGG 2 cut(s) 299, 591
BssMI GATC 6 cut(s) 196, 253, 330, 338, 486, 600
Bst2UI CCWGG 2 cut(s) 300, 593
Bst4CI ACNGT 4 cut(s) 57, 83, 293, 527
Bst6I CTCTTC 1 cut(s) 545
BstAFI CTTAAG 1 cut(s) 170
BstC8I GCNNGC 2 cut(s) 72, 581
BstDEI CTNAG 2 cut(s) 423, 606
BstEII GGTNACC 1 cut(s) 538
BstF5I GGATG 4 cut(s) 82, 166, 309, 587
BstH2I RGCGCY 1 cut(s) 559
BstHHI GCGC 1 cut(s) 558
BstKTI GATC 6 cut(s) 199, 256, 333, 341, 489, 603
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 6 cut(s) 196, 253, 330, 338, 486, 600
BstMWI GCNNNNNNNGC 1 cut(s) 475
BstNI CCWGG 2 cut(s) 300, 593
BstNSI RCATGY 1 cut(s) 406
BstPI GGTNACC 1 cut(s) 538
BstSCI CCNGG 2 cut(s) 298, 591
BstX2I RGATCY 1 cut(s) 338
BstYI RGATCY 1 cut(s) 338
Bsu15I ATCGAT 1 cut(s) 195
BsuTUI ATCGAT 1 cut(s) 195
BtsCI GGATG 4 cut(s) 82, 166, 309, 587
BtsIMutI CAGTG 2 cut(s) 88, 519
Cac8I GCNNGC 2 cut(s) 72, 581
CfoI GCGC 1 cut(s) 558
Cfr10I RCCGGY 1 cut(s) 579
Cfr13I GGNCC 1 cut(s) 263
ClaI ATCGAT 1 cut(s) 195
CseI GACGC 1 cut(s) 118
Csp6I GTAC 2 cut(s) 356, 386
CviAII CATG 1 cut(s) 403
CviJI RGCY 8 cut(s) 88, 167, 422, 442, 469, 478, 583, 615
CviKI_1 RGCY 8 cut(s) 88, 167, 422, 442, 469, 478, 583, 615
CviQI GTAC 2 cut(s) 356, 386
DdeI CTNAG 2 cut(s) 423, 606
DpnI GATC 6 cut(s) 198, 255, 332, 340, 488, 602
DpnII GATC 6 cut(s) 196, 253, 330, 338, 486, 600
Eam1104I CTCTTC 1 cut(s) 545
EarI CTCTTC 1 cut(s) 545
EciI GGCGGA 1 cut(s) 566
Eco32I GATATC 1 cut(s) 193
Eco47I GGWCC 1 cut(s) 263
Eco91I GGTNACC 1 cut(s) 538
EcoO65I GGTNACC 1 cut(s) 538
EcoRI GAATTC 1 cut(s) 241
EcoRII CCWGG 2 cut(s) 298, 591
EcoRV GATATC 1 cut(s) 193
Esp3I CGTCTC 1 cut(s) 101
FaeI CATG 1 cut(s) 406
FaiI YATR 4 cut(s) 296, 315, 371, 404
FaqI GGGAC 1 cut(s) 276
FatI CATG 1 cut(s) 402
FbaI TGATCA 1 cut(s) 486
FokI GGATG 4 cut(s) 89, 173, 316, 574
GlaI GCGC 1 cut(s) 557
HaeII RGCGCY 1 cut(s) 559
HapII CCGG 2 cut(s) 507, 580
HgaI GACGC 1 cut(s) 118
HhaI GCGC 1 cut(s) 558
Hin1II CATG 1 cut(s) 406
Hin6I GCGC 1 cut(s) 556
HinP1I GCGC 1 cut(s) 556
HinfI GANTC 3 cut(s) 38, 152, 218
HpaII CCGG 2 cut(s) 507, 580
Hpy188I TCNGA 5 cut(s) 22, 104, 217, 253, 457
Hpy188III TCNNGA 1 cut(s) 257
HpyAV CCTTC 3 cut(s) 139, 399, 410
HpyCH4III ACNGT 4 cut(s) 57, 83, 293, 527
HpyCH4IV ACGT 1 cut(s) 121
HpyF10VI GCNNNNNNNGC 1 cut(s) 475
HpyF3I CTNAG 2 cut(s) 423, 606
HpySE526I ACGT 1 cut(s) 121
Hsp92II CATG 1 cut(s) 406
HspAI GCGC 1 cut(s) 556
KroI GCCGGC 1 cut(s) 579
KroNI GCCGGC 1 cut(s) 581
Ksp22I TGATCA 1 cut(s) 486
Kzo9I GATC 6 cut(s) 196, 253, 330, 338, 486, 600
LmnI GCTCC 1 cut(s) 117
MaeII ACGT 1 cut(s) 121
MaeIII GTNAC 3 cut(s) 28, 509, 538
MalI GATC 6 cut(s) 198, 255, 332, 340, 488, 602
MbiI CCGCTC 1 cut(s) 309
MboI GATC 6 cut(s) 196, 253, 330, 338, 486, 600
MboII GAAGA 3 cut(s) 204, 425, 562
MflI RGATCY 1 cut(s) 338
MluCI AATT 4 cut(s) 23, 241, 271, 471
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 1 cut(s) 251
MnlI CCTC 5 cut(s) 253, 418, 529, 546, 601
MroNI GCCGGC 1 cut(s) 579
MseI TTAA 2 cut(s) 117, 171
MspCI CTTAAG 1 cut(s) 170
MspI CCGG 2 cut(s) 507, 580
MspR9I CCNGG 2 cut(s) 300, 593
MvaI CCWGG 2 cut(s) 300, 593
MwoI GCNNNNNNNGC 1 cut(s) 475
NaeI GCCGGC 1 cut(s) 581
NdeII GATC 6 cut(s) 196, 253, 330, 338, 486, 600
NgoMIV GCCGGC 1 cut(s) 579
NlaIII CATG 1 cut(s) 406
NlaIV GGNNCC 1 cut(s) 264
NspI RCATGY 1 cut(s) 406
PdiI GCCGGC 1 cut(s) 581
PfeI GAWTC 2 cut(s) 152, 218
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
Psp1406I AACGTT 1 cut(s) 121
Psp6I CCWGG 2 cut(s) 298, 591
PspEI GGTNACC 1 cut(s) 538
PspGI CCWGG 2 cut(s) 298, 591
PspN4I GGNNCC 1 cut(s) 264
PspPI GGNCC 1 cut(s) 263
PsuI RGATCY 1 cut(s) 338
RsaI GTAC 2 cut(s) 357, 387
RsaNI GTAC 2 cut(s) 356, 386
SaqAI TTAA 2 cut(s) 117, 171
Sau3AI GATC 6 cut(s) 196, 253, 330, 338, 486, 600
Sau96I GGNCC 1 cut(s) 263
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 2 cut(s) 300, 593
SinI GGWCC 1 cut(s) 263
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 4 cut(s) 23, 241, 271, 471
SsiI CCGC 3 cut(s) 74, 309, 577
StyD4I CCNGG 2 cut(s) 298, 591
TaaI ACNGT 4 cut(s) 57, 83, 293, 527
TaiI ACGT 1 cut(s) 124
TaqI TCGA 1 cut(s) 195
TasI AATT 4 cut(s) 23, 241, 271, 471
TfiI GAWTC 2 cut(s) 152, 218
Tru1I TTAA 2 cut(s) 117, 171
Tru9I TTAA 2 cut(s) 117, 171
TscAI CASTG 2 cut(s) 88, 526
TspDTI ATGAA 3 cut(s) 177, 234, 556
TspGWI ACGGA 1 cut(s) 343
TspRI CASTG 2 cut(s) 88, 526
Vha464I CTTAAG 1 cut(s) 170
VpaK11BI GGWCC 1 cut(s) 263
XapI RAATTY 1 cut(s) 241
XceI RCATGY 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.