Rh5AG338900

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0004501
Physical Location & Seq
Forward (+)
54885992 .. 54887575
1584 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG338900.1

Sequence Viewer

Length: 345 bp
ATGGCGCTAATGATGGGTGGAGATTCGGGGATATCTGCGTTCCCTGAAGAAGACAACATATTCTGCTGGAAAGGGACCATCAATGGAAGCAAAGAGACAGTTTTCGAAGGGACTCAATACAGATTGTCCCTTTCTTTCCCCAATGACTATCCCTTTAAGCCTCCCAAGGTCAAGTTCGAGTCTCTCTGTTTCCATCCCAATGTCGATGTCCGCGGCCACATTTGCTTGGATATTCTTCAGGATAAATGGTCTTCGGCTTATGATGTGAGAACCATCCTCTTATCGATCCAGAGTCTTCTAGGAGGTAATCTCTTACTGGTTTTCTTTGTCTATGCTGCTGTGTGA

Protein Analysis

114

Amino Acids

12.77

Weight (kDa)

5.18

Isoelectric Point (pI)

44.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 8 - 100 6.9e-37 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 213
AciI CCGC 2 cut(s) 211, 213
AclWI GGATC 1 cut(s) 280
AcoI YGGCCR 1 cut(s) 214
AcuI CTGAAG 2 cut(s) 66, 221
AjuI GAANNNNNNNTTGG 2 cut(s) 158, 190
Alw26I GTCTC 2 cut(s) 89, 186
AlwI GGATC 1 cut(s) 280
AoxI GGCC 1 cut(s) 214
ApeKI GCWGC 1 cut(s) 335
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 75
AsuII TTCGAA 1 cut(s) 105
AvaII GGWCC 1 cut(s) 75
BbsI GAAGAC 3 cut(s) 57, 243, 287
BbvI GCAGC 1 cut(s) 322
BccI CCATC 4 cut(s) 7, 86, 201, 281
BcoDI GTCTC 2 cut(s) 89, 186
BfaI CTAG 1 cut(s) 299
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 2 cut(s) 214, 336
BlsI GCNGC 2 cut(s) 215, 337
Bme18I GGWCC 1 cut(s) 75
BmgT120I GGNCC 1 cut(s) 75
BmiI GGNNCC 1 cut(s) 76
BpiI GAAGAC 3 cut(s) 57, 243, 287
BplI GAGNNNNNCTC 2 cut(s) 294, 326
Bpu14I TTCGAA 1 cut(s) 105
Bsa29I ATCGAT 1 cut(s) 284
BsaJI CCNNGG 2 cut(s) 165, 211
Bse1I ACTGG 1 cut(s) 321
BseCI ATCGAT 1 cut(s) 284
BseDI CCNNGG 2 cut(s) 165, 211
BseGI GGATG 2 cut(s) 193, 273
BseNI ACTGG 1 cut(s) 321
BseXI GCAGC 1 cut(s) 322
Bsh1236I CGCG 1 cut(s) 213
BshFI GGCC 1 cut(s) 216
BshVI ATCGAT 1 cut(s) 284
BslFI GGGAC 3 cut(s) 88, 112, 124
BsmAI GTCTC 2 cut(s) 89, 186
BsmFI GGGAC 3 cut(s) 88, 112, 124
BsnI GGCC 1 cut(s) 216
Bsp119I TTCGAA 1 cut(s) 105
Bsp143I GATC 1 cut(s) 285
BspACI CCGC 2 cut(s) 211, 213
BspANI GGCC 1 cut(s) 216
BspDI ATCGAT 1 cut(s) 284
BspFNI CGCG 1 cut(s) 213
BspLI GGNNCC 1 cut(s) 76
BspPI GGATC 1 cut(s) 280
BspT104I TTCGAA 1 cut(s) 105
BsrI ACTGG 1 cut(s) 321
BssECI CCNNGG 2 cut(s) 165, 211
BssMI GATC 1 cut(s) 285
BssT1I CCWWGG 1 cut(s) 165
Bst4CI ACNGT 1 cut(s) 100
BstBI TTCGAA 1 cut(s) 105
BstDSI CCRYGG 1 cut(s) 211
BstF5I GGATG 2 cut(s) 193, 273
BstFNI CGCG 1 cut(s) 213
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 1 cut(s) 288
BstMAI GTCTC 2 cut(s) 89, 186
BstMBI GATC 1 cut(s) 285
BstMWI GCNNNNNNNGC 1 cut(s) 222
BstUI CGCG 1 cut(s) 213
BstV1I GCAGC 1 cut(s) 322
BstV2I GAAGAC 3 cut(s) 57, 243, 287
Bsu15I ATCGAT 1 cut(s) 284
BsuRI GGCC 1 cut(s) 216
BsuTUI ATCGAT 1 cut(s) 284
BtgI CCRYGG 1 cut(s) 211
BtsCI GGATG 2 cut(s) 193, 273
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 75
Cfr42I CCGCGG 1 cut(s) 214
ClaI ATCGAT 1 cut(s) 284
CviJI RGCY 3 cut(s) 160, 216, 257
CviKI_1 RGCY 3 cut(s) 160, 216, 257
DpnI GATC 1 cut(s) 287
DpnII GATC 1 cut(s) 285
EaeI YGGCCR 1 cut(s) 214
Eco130I CCWWGG 1 cut(s) 165
Eco32I GATATC 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 75
Eco57I CTGAAG 2 cut(s) 66, 221
EcoRV GATATC 1 cut(s) 33
EcoT14I CCWWGG 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 165
FaiI YATR 3 cut(s) 59, 261, 333
FaqI GGGAC 3 cut(s) 88, 112, 124
Fnu4HI GCNGC 2 cut(s) 214, 336
FokI GGATG 2 cut(s) 180, 260
Fsp4HI GCNGC 2 cut(s) 214, 336
FspBI CTAG 1 cut(s) 299
GlaI GCGC 1 cut(s) 6
GluI GCNGC 2 cut(s) 214, 336
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 216
HhaI GCGC 1 cut(s) 7
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 4 cut(s) 23, 112, 179, 292
Hpy188III TCNNGA 2 cut(s) 239, 289
HpyAV CCTTC 1 cut(s) 101
HpyCH4III ACNGT 1 cut(s) 100
HpyF10VI GCNNNNNNNGC 1 cut(s) 222
HspAI GCGC 1 cut(s) 5
KspI CCGCGG 1 cut(s) 214
Kzo9I GATC 1 cut(s) 285
LpnPI CCDG 5 cut(s) 52, 57, 224, 302, 302
Lsp1109I GCAGC 1 cut(s) 322
MaeI CTAG 1 cut(s) 299
MalI GATC 1 cut(s) 287
MboI GATC 1 cut(s) 285
MboII GAAGA 5 cut(s) 59, 62, 227, 243, 287
MlyI GAGTC 3 cut(s) 106, 188, 301
MnlI CCTC 3 cut(s) 171, 287, 296
MseI TTAA 1 cut(s) 156
MslI CAYNNNNRTG 1 cut(s) 198
MspA1I CMGCKG 1 cut(s) 213
MvnI CGCG 1 cut(s) 213
MwoI GCNNNNNNNGC 1 cut(s) 222
NdeII GATC 1 cut(s) 285
NlaIV GGNNCC 1 cut(s) 76
NspV TTCGAA 1 cut(s) 105
PfeI GAWTC 1 cut(s) 23
PkrI GCNGC 2 cut(s) 215, 337
PleI GAGTC 3 cut(s) 106, 187, 300
PpsI GAGTC 3 cut(s) 106, 187, 300
PspN4I GGNNCC 1 cut(s) 76
PspPI GGNCC 1 cut(s) 75
RseI CAYNNNNRTG 1 cut(s) 198
SacII CCGCGG 1 cut(s) 214
SaqAI TTAA 1 cut(s) 156
SatI GCNGC 2 cut(s) 214, 336
Sau3AI GATC 1 cut(s) 285
Sau96I GGNCC 1 cut(s) 75
SchI GAGTC 3 cut(s) 106, 188, 301
SetI ASST 2 cut(s) 171, 307
Sfr303I CCGCGG 1 cut(s) 214
SfuI TTCGAA 1 cut(s) 105
SgrBI CCGCGG 1 cut(s) 214
SinI GGWCC 1 cut(s) 75
SmiMI CAYNNNNRTG 1 cut(s) 198
SsiI CCGC 2 cut(s) 211, 213
SspMI CTAG 1 cut(s) 299
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 1 cut(s) 100
TaqI TCGA 4 cut(s) 105, 177, 204, 284
TauI GCSGC 1 cut(s) 216
TfiI GAWTC 1 cut(s) 23
Tru1I TTAA 1 cut(s) 156
Tru9I TTAA 1 cut(s) 156
TseI GCWGC 1 cut(s) 335
VpaK11BI GGWCC 1 cut(s) 75
XspI CTAG 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.