Rh5AG521000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0006966
Physical Location & Seq
Reverse (-)
89267228 .. 89275780
8553 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG521000.1

Sequence Viewer

Length: 630 bp
ATGGCAAAAGGCCCATCTTTGTCCCAATATGGCCTCTTCTTCTTCCTTCGTCTCATCTCTGAGCCTCTCTCACTCTTCATAACTGAGCTCTCTCAATTTCTAAATTTTCCGATTTTGAGCGAGGAGCCAAATAGAGATCTGGAATTGTCGGTGGGGCCTAGGTTTCATGGTGGCGATGACGATTGGGAAGACGAAGCTGCACTCTGGCTGGCTCGGCGCCACAAACAGGGATGTCATGAGAACAATGGAGCCATAAATGTTCCTATACGGAAAACCATTCAAGCTTTTCTCGTTTATTTTCATAATCAAGACGATGAATGTGTTCCCAATCCTTCGCCTGATAGCAGAAGATTTGAGACATCAAATGGTAAAGCTTGCTATACGAAGTCAAGAAAAAGAAAAGGAATGAAGGATGATGAAGATCCTTCCAAATTTCCATCTATTCCAGAGGAACCCCCTTATGAAAGGGTCATCAGGTTTTACACCATAATTGGGAGACCAGATGATCCTCATAAATCAAAGCTTAGATCATGCAAAGGAAAAGAAGTGAAGGCAAATACTCTTGCTATTAAGATGGCTGTTAGTTTTCTTTGCATGGAGCTGAAGTTCCAGTTTTACCAAAAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

209

Amino Acids

24.11

Weight (kDa)

8.22

Isoelectric Point (pI)

60.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 216
AclWI GGATC 2 cut(s) 416, 500
AcsI RAATTY 2 cut(s) 103, 431
AcuI CTGAAG 1 cut(s) 623
AcyI GRCGYC 1 cut(s) 217
AfiI CCNNNNNNNGG 2 cut(s) 226, 492
AgsI TTSAA 1 cut(s) 281
AloI GAACNNNNNNTCC 2 cut(s) 590, 622
AluBI AGCT 6 cut(s) 88, 197, 284, 374, 523, 601
AluI AGCT 6 cut(s) 88, 197, 284, 374, 523, 601
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 3 cut(s) 56, 350, 490
AlwI GGATC 2 cut(s) 416, 500
AoxI GGCC 3 cut(s) 10, 31, 155
ApeKI GCWGC 1 cut(s) 197
ApoI RAATTY 2 cut(s) 103, 431
Asp700I GAANNNNTTC 1 cut(s) 321
AspA2I CCTAGG 1 cut(s) 158
AspLEI GCGC 1 cut(s) 219
AspS9I GGNCC 2 cut(s) 11, 155
AvrII CCTAGG 1 cut(s) 158
BanI GGYRCC 1 cut(s) 216
BanII GRGCYC 1 cut(s) 90
BbsI GAAGAC 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 184
BccI CCATC 3 cut(s) 22, 445, 568
BcoDI GTCTC 3 cut(s) 56, 350, 490
BfaI CTAG 1 cut(s) 159
BfoI RGCGCY 1 cut(s) 220
BglII AGATCT 1 cut(s) 136
BisI GCNGC 1 cut(s) 198
BlnI CCTAGG 1 cut(s) 158
BlsI GCNGC 1 cut(s) 199
BmgT120I GGNCC 2 cut(s) 11, 155
BmiI GGNNCC 5 cut(s) 126, 156, 218, 250, 453
BpiI GAAGAC 1 cut(s) 195
BplI GAGNNNNNCTC 2 cut(s) 53, 85
BsaBI GATNNNNATC 1 cut(s) 420
BsaHI GRCGYC 1 cut(s) 217
BsaI GGTCTC 1 cut(s) 490
BsaJI CCNNGG 1 cut(s) 158
BsaXI ACNNNNNCTCC 2 cut(s) 590, 620
Bsc4I CCNNNNNNNGG 2 cut(s) 226, 492
Bse1I ACTGG 1 cut(s) 610
Bse8I GATNNNNATC 1 cut(s) 420
BseDI CCNNGG 1 cut(s) 158
BseGI GGATG 2 cut(s) 236, 418
BseJI GATNNNNATC 1 cut(s) 420
BseLI CCNNNNNNNGG 2 cut(s) 226, 492
BseMII CTCAG 2 cut(s) 51, 75
BseNI ACTGG 1 cut(s) 610
BseRI GAGGAG 1 cut(s) 137
BseXI GCAGC 1 cut(s) 184
BsgI GTGCAG 1 cut(s) 183
BshFI GGCC 3 cut(s) 12, 33, 157
BshNI GGYRCC 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 90
BslFI GGGAC 1 cut(s) 7
BslI CCNNNNNNNGG 2 cut(s) 226, 492
BsmAI GTCTC 3 cut(s) 56, 350, 490
BsmBI CGTCTC 1 cut(s) 56
BsmFI GGGAC 1 cut(s) 7
BsnI GGCC 3 cut(s) 12, 33, 157
Bso31I GGTCTC 1 cut(s) 490
Bsp1286I GDGCHC 1 cut(s) 90
Bsp143I GATC 4 cut(s) 136, 421, 505, 527
BspANI GGCC 3 cut(s) 12, 33, 157
BspCNI CTCAG 2 cut(s) 52, 76
BspHI TCATGA 1 cut(s) 235
BspLI GGNNCC 5 cut(s) 126, 156, 218, 250, 453
BspPI GGATC 2 cut(s) 416, 500
BspT107I GGYRCC 1 cut(s) 216
BspTNI GGTCTC 1 cut(s) 490
BsrI ACTGG 1 cut(s) 610
BssECI CCNNGG 1 cut(s) 158
BssMI GATC 4 cut(s) 136, 421, 505, 527
BssNI GRCGYC 1 cut(s) 217
BssT1I CCWWGG 1 cut(s) 158
Bst6I CTCTTC 2 cut(s) 41, 80
BstACI GRCGYC 1 cut(s) 217
BstC8I GCNNGC 2 cut(s) 210, 376
BstDEI CTNAG 3 cut(s) 60, 84, 524
BstF5I GGATG 2 cut(s) 236, 418
BstH2I RGCGCY 1 cut(s) 220
BstHHI GCGC 1 cut(s) 219
BstKTI GATC 4 cut(s) 139, 424, 508, 530
BstMAI GTCTC 3 cut(s) 56, 350, 490
BstMBI GATC 4 cut(s) 136, 421, 505, 527
BstMWI GCNNNNNNNGC 1 cut(s) 214
BstV1I GCAGC 1 cut(s) 184
BstV2I GAAGAC 1 cut(s) 195
BstX2I RGATCY 2 cut(s) 136, 421
BstYI RGATCY 2 cut(s) 136, 421
BsuRI GGCC 3 cut(s) 12, 33, 157
BtgZI GCGATG 1 cut(s) 189
BtsCI GGATG 2 cut(s) 236, 418
Cac8I GCNNGC 2 cut(s) 210, 376
CciI TCATGA 1 cut(s) 235
CfoI GCGC 1 cut(s) 219
Cfr13I GGNCC 2 cut(s) 11, 155
CviAII CATG 4 cut(s) 167, 236, 531, 595
DdeI CTNAG 3 cut(s) 60, 84, 524
DinI GGCGCC 1 cut(s) 218
DpnI GATC 4 cut(s) 138, 423, 507, 529
DpnII GATC 4 cut(s) 136, 421, 505, 527
Eam1104I CTCTTC 2 cut(s) 41, 80
EarI CTCTTC 2 cut(s) 41, 80
Ecl136II GAGCTC 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 158
Eco24I GRGCYC 1 cut(s) 90
Eco31I GGTCTC 1 cut(s) 490
Eco53kI GAGCTC 1 cut(s) 88
Eco57I CTGAAG 1 cut(s) 623
EcoICRI GAGCTC 1 cut(s) 88
EcoO109I RGGNCCY 1 cut(s) 155
EcoT14I CCWWGG 1 cut(s) 158
EcoT38I GRGCYC 1 cut(s) 90
EgeI GGCGCC 1 cut(s) 218
EheI GGCGCC 1 cut(s) 218
ErhI CCWWGG 1 cut(s) 158
Esp3I CGTCTC 1 cut(s) 56
FaeI CATG 4 cut(s) 170, 239, 534, 598
FaqI GGGAC 1 cut(s) 7
FatI CATG 4 cut(s) 166, 235, 530, 594
Fnu4HI GCNGC 1 cut(s) 198
FokI GGATG 2 cut(s) 243, 425
FriOI GRGCYC 1 cut(s) 90
Fsp4HI GCNGC 1 cut(s) 198
FspBI CTAG 1 cut(s) 159
GlaI GCGC 1 cut(s) 218
GluI GCNGC 1 cut(s) 198
HaeII RGCGCY 1 cut(s) 220
HaeIII GGCC 3 cut(s) 12, 33, 157
HhaI GCGC 1 cut(s) 219
Hin1I GRCGYC 1 cut(s) 217
Hin1II CATG 4 cut(s) 170, 239, 534, 598
Hin6I GCGC 1 cut(s) 217
HinP1I GCGC 1 cut(s) 217
HindIII AAGCTT 3 cut(s) 282, 372, 521
Hpy188I TCNGA 2 cut(s) 61, 111
Hpy188III TCNNGA 6 cut(s) 140, 236, 308, 390, 446, 627
HpyAV CCTTC 5 cut(s) 56, 342, 403, 435, 544
HpyCH4V TGCA 3 cut(s) 200, 534, 594
HpyF10VI GCNNNNNNNGC 1 cut(s) 214
HpyF3I CTNAG 3 cut(s) 60, 84, 524
Hsp92I GRCGYC 1 cut(s) 217
Hsp92II CATG 4 cut(s) 170, 239, 534, 598
HspAI GCGC 1 cut(s) 217
KasI GGCGCC 1 cut(s) 216
Kzo9I GATC 4 cut(s) 136, 421, 505, 527
LmnI GCTCC 3 cut(s) 124, 248, 598
LpnPI CCDG 9 cut(s) 125, 190, 194, 212, 351, 459, 460, 513, 623
Lsp1109I GCAGC 1 cut(s) 184
MaeI CTAG 1 cut(s) 159
MalI GATC 4 cut(s) 138, 423, 507, 529
MboI GATC 4 cut(s) 136, 421, 505, 527
MboII GAAGA 7 cut(s) 28, 31, 34, 67, 200, 360, 431
MflI RGATCY 2 cut(s) 136, 421
MhlI GDGCHC 1 cut(s) 90
MluCI AATT 5 cut(s) 95, 103, 143, 431, 489
Mly113I GGCGCC 1 cut(s) 217
MnlI CCTC 5 cut(s) 44, 75, 115, 442, 519
MroXI GAANNNNTTC 1 cut(s) 321
MseI TTAA 1 cut(s) 570
MwoI GCNNNNNNNGC 1 cut(s) 214
NarI GGCGCC 1 cut(s) 217
NdeII GATC 4 cut(s) 136, 421, 505, 527
NlaIII CATG 4 cut(s) 170, 239, 534, 598
NlaIV GGNNCC 5 cut(s) 126, 156, 218, 250, 453
NmeAIII GCCGAG 1 cut(s) 193
PagI TCATGA 1 cut(s) 235
PdmI GAANNNNTTC 1 cut(s) 321
PkrI GCNGC 1 cut(s) 199
PluTI GGCGCC 1 cut(s) 220
Psp124BI GAGCTC 1 cut(s) 90
PspN4I GGNNCC 5 cut(s) 126, 156, 218, 250, 453
PspPI GGNCC 2 cut(s) 11, 155
PsuI RGATCY 2 cut(s) 136, 421
SacI GAGCTC 1 cut(s) 90
SaqAI TTAA 1 cut(s) 570
SatI GCNGC 1 cut(s) 198
Sau3AI GATC 4 cut(s) 136, 421, 505, 527
Sau96I GGNCC 2 cut(s) 11, 155
SduI GDGCHC 1 cut(s) 90
SetI ASST 8 cut(s) 90, 164, 199, 286, 376, 479, 525, 603
SfoI GGCGCC 1 cut(s) 218
Sse9I AATT 5 cut(s) 95, 103, 143, 431, 489
SspDI GGCGCC 1 cut(s) 216
SspMI CTAG 1 cut(s) 159
SstI GAGCTC 1 cut(s) 90
StyI CCWWGG 1 cut(s) 158
TasI AATT 5 cut(s) 95, 103, 143, 431, 489
Tru1I TTAA 1 cut(s) 570
Tru9I TTAA 1 cut(s) 570
TseI GCWGC 1 cut(s) 197
TspDTI ATGAA 7 cut(s) 67, 155, 290, 330, 422, 432, 477
TspGWI ACGGA 1 cut(s) 283
XapI RAATTY 2 cut(s) 103, 431
XmaJI CCTAGG 1 cut(s) 158
XmnI GAANNNNTTC 1 cut(s) 321
XspI CTAG 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.