Rh5BG189500

Endoplasmic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0009858
Physical Location & Seq
Reverse (-)
20846746 .. 20850688
3943 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG189500.1

Sequence Viewer

Length: 1470 bp
ATGTCTCTTAGTCCTTCGCTACAGCATTGGCGCGTGGAATTGGAGATGGTGAAGGAGAAGAATGTGAAGAGATGGGTTTTAGTGGGTGCTGTGGTTGTGGTGTTATTAGCCATAGCTCTCACCTCTACCAGAGATTTTAATTCTAAACTCAACCTTTCTCTGTTTTTCAAGAATCCCAGTTCCTGCCAATGTCCTCAGGATTCAAAGAAATACAGTGGCCTTGTTGAGGACTGCTGTTGTGATTATGAAACGGTGGACAGTGTTAATGCAGAGGTGTTGCATCCTCTGCTACAAGAGATTGTTAAAACTCCGTTTTTTCGATATTTTAAGGCCAAGCTGTGGTGTGACTGTCCCTTCTGGACTGAAGATGGTATGTGCCTTCTGCGTGACTGCAGCGTCTGCGAATGCCCAGAAAATGAATTTCCGGAACCTTTCAAGAGGCCGTTTCACCGTGGCCTTCCATCCGACAGTTTGGTTTGTCAAGATGATAAGCTGCAGGGGTCTGTTGACCGCACTCTAGACAGTAGGGCATTCAGAGGCTGGATTGAGACAGATAATCCTTGGACAAATGATGACGAGACTGATAATGATGAGATGACATATGTGAACCTTCTACTGAATCCTGAACGTTACACTGGCTATACTGGTCCATCAGCAAGAAGGATATGGGATGCTGTTTATTCTGAAAATTGCCCAAAATATTCATCTCAGGACATTTGCCAGGAGCAAAGAGTATTGTACAAATTAATATCGGGTCTTCACTCATCAATTTCAATCCACATAGCTGCTGATTATCTTCTAGACAAGATTACCAATCTGTGGGGTCAAAACATGGAATTGATGCATGATCGCGTTTTAAAATATCCAGATCGTGTTCAAAATTTGTACTTCGCATTCCTCTTTGTTCTCCGGGCTGTGACAAAAGCTGCAGATTACTTAGAGCAGGCTGAGTATGATACAGGTAATCATGAGGAAGACCTGAGAACAGAGTCTTTGATGAGGCAGCTACTTTACAATCCTAAACTCCAAGCTGCATGTCCCCTACCGTTTGATGAAGCAAAGCTCTGGAAAGGTCAGAGCGGACCCCAATTGAAGCAACAAATTCAAAAGAAATTCAGAAACATTAGTGCATTAATGGATTGTGTGGGATGTGAGAAATGTCGGCTCTGGGGAAAGCTTCAAGTTCTTGGTCTTGGTACTGCATTGAAGATCCTCTTTTCTGTTGATGGTAAAAATCATCAAGATCAACCTCTGCAGCTGCAACGAAATGAAGTGATAGCTCTCGTAAATCTGCTAAATCGACTCTCTGAATCTGTCAAATATGCTTGTCAAAGCAGACATTCAATCAAGAAAGTAGCAGAAGGAGGACGAGTGGTTGAACCTTATTCTATACAACCAATCAGTACATTGCAAAGAATATGGGAGGCGGTGATCCCGATCAGGCTTAGGTTAGGGATGTTAGCACTGTAA

Protein Analysis

489

Amino Acids

56.21

Weight (kDa)

6.2

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERO1 PF04137 93 - 441 1.7e-122 Endoplasmic Reticulum Oxidoreductin 1 (ERO1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 395
AccB7I CCANNNNNTGG 2 cut(s) 339, 819
AccBSI CCGCTC 1 cut(s) 1080
AccII CGCG 2 cut(s) 33, 852
AccIII TCCGGA 1 cut(s) 424
AciI CCGC 3 cut(s) 511, 1080, 1427
AclI AACGTT 1 cut(s) 628
AclWI GGATC 2 cut(s) 1204, 1426
AcsI RAATTY 4 cut(s) 419, 880, 1101, 1112
AcuI CTGAAG 1 cut(s) 384
AfaI GTAC 4 cut(s) 740, 887, 1198, 1405
AfiI CCNNNNNNNGG 3 cut(s) 226, 339, 819
AjnI CCWGG 1 cut(s) 720
Alw26I GTCTC 3 cut(s) 9, 542, 572
AlwI GGATC 2 cut(s) 1204, 1426
AlwNI CAGNNNCTG 3 cut(s) 183, 399, 540
Aor13HI TCCGGA 1 cut(s) 424
AoxI GGCC 4 cut(s) 217, 330, 440, 454
ApeKI GCWGC 8 cut(s) 393, 493, 785, 926, 1003, 1031, 1255, 1258
ApoI RAATTY 4 cut(s) 419, 880, 1101, 1112
AseI ATTAAT 2 cut(s) 746, 1133
AspLEI GCGC 1 cut(s) 33
AspS9I GGNCC 2 cut(s) 647, 1082
AsuC2I CCSGG 1 cut(s) 911
AsuHPI GGTGA 4 cut(s) 61, 112, 440, 1441
AvaII GGWCC 2 cut(s) 647, 1082
AxyI CCTNAGG 1 cut(s) 195
BbsI GAAGAC 2 cut(s) 749, 981
BbvI GCAGC 8 cut(s) 405, 480, 772, 913, 1015, 1018, 1245, 1267
BccI CCATC 6 cut(s) 40, 66, 362, 469, 658, 1220
BceAI ACGGC 1 cut(s) 427
BciT130I CCWGG 1 cut(s) 722
BcnI CCSGG 1 cut(s) 911
BcoDI GTCTC 3 cut(s) 9, 542, 572
BfaI CTAG 2 cut(s) 518, 800
BfmI CTRYAG 5 cut(s) 20, 391, 494, 927, 1253
BisI GCNGC 8 cut(s) 394, 494, 786, 927, 1004, 1032, 1256, 1259
BlsI GCNGC 8 cut(s) 395, 495, 787, 928, 1005, 1033, 1257, 1260
Bme1390I CCNGG 2 cut(s) 722, 911
Bme18I GGWCC 2 cut(s) 647, 1082
BmgT120I GGNCC 2 cut(s) 647, 1082
BmiI GGNNCC 2 cut(s) 429, 1084
BmrFI CCNGG 2 cut(s) 722, 911
BmrI ACTGGG 1 cut(s) 171
BmsI GCATC 3 cut(s) 289, 661, 831
BmuI ACTGGG 1 cut(s) 171
BpiI GAAGAC 2 cut(s) 749, 981
Bpu10I CCTNAGC 1 cut(s) 1445
BpuMI CCSGG 1 cut(s) 911
BsaBI GATNNNNATC 1 cut(s) 1436
BsaJI CCNNGG 2 cut(s) 451, 560
BsaWI WCCGGW 1 cut(s) 424
BsaXI ACNNNNNCTCC 2 cut(s) 1356, 1386
Bsc4I CCNNNNNNNGG 3 cut(s) 226, 339, 819
Bse1I ACTGG 3 cut(s) 177, 640, 649
Bse21I CCTNAGG 1 cut(s) 195
Bse3DI GCAATG 1 cut(s) 1406
Bse8I GATNNNNATC 1 cut(s) 1436
BseAI TCCGGA 1 cut(s) 424
BseBI CCWGG 1 cut(s) 722
BseDI CCNNGG 2 cut(s) 451, 560
BseGI GGATG 5 cut(s) 280, 461, 676, 1154, 1461
BseJI GATNNNNATC 1 cut(s) 1436
BseLI CCNNNNNNNGG 3 cut(s) 226, 339, 819
BseMI GCAATG 1 cut(s) 1406
BseMII CTCAG 4 cut(s) 209, 722, 939, 971
BseNI ACTGG 3 cut(s) 177, 640, 649
BseXI GCAGC 8 cut(s) 405, 480, 772, 913, 1015, 1018, 1245, 1267
Bsh1236I CGCG 2 cut(s) 33, 852
BshFI GGCC 4 cut(s) 219, 332, 442, 456
BsiSI CCGG 2 cut(s) 425, 910
BslFI GGGAC 2 cut(s) 336, 1023
BslI CCNNNNNNNGG 3 cut(s) 226, 339, 819
BsmAI GTCTC 3 cut(s) 9, 542, 572
BsmFI GGGAC 2 cut(s) 336, 1023
BsmI GAATGC 3 cut(s) 410, 530, 893
BsnI GGCC 4 cut(s) 219, 332, 442, 456
Bsp13I TCCGGA 1 cut(s) 424
Bsp1407I TGTACA 1 cut(s) 738
Bsp143I GATC 6 cut(s) 847, 868, 1209, 1243, 1431, 1437
BspACI CCGC 3 cut(s) 511, 1080, 1427
BspANI GGCC 4 cut(s) 219, 332, 442, 456
BspCNI CTCAG 4 cut(s) 208, 721, 940, 972
BspEI TCCGGA 1 cut(s) 424
BspFNI CGCG 2 cut(s) 33, 852
BspHI TCATGA 1 cut(s) 967
BspLI GGNNCC 2 cut(s) 429, 1084
BspMAI CTGCAG 4 cut(s) 395, 498, 931, 1257
BspPI GGATC 2 cut(s) 1204, 1426
BsrBI CCGCTC 1 cut(s) 1080
BsrDI GCAATG 1 cut(s) 1406
BsrGI TGTACA 1 cut(s) 738
BsrI ACTGG 3 cut(s) 177, 640, 649
BssECI CCNNGG 2 cut(s) 451, 560
BssMI GATC 6 cut(s) 847, 868, 1209, 1243, 1431, 1437
BssT1I CCWWGG 1 cut(s) 560
Bst2UI CCWGG 1 cut(s) 722
Bst4CI ACNGT 9 cut(s) 215, 253, 260, 350, 452, 470, 524, 1047, 1467
Bst6I CTCTTC 1 cut(s) 62
BstAPI GCANNNNNTGC 2 cut(s) 286, 399
BstAUI TGTACA 1 cut(s) 738
BstC8I GCNNGC 1 cut(s) 945
BstDEI CTNAG 7 cut(s) 8, 195, 708, 937, 948, 980, 1445
BstDSI CCRYGG 1 cut(s) 451
BstENI CCTNNNNNAGG 1 cut(s) 224
BstF5I GGATG 5 cut(s) 280, 461, 676, 1154, 1461
BstFNI CGCG 2 cut(s) 33, 852
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 6 cut(s) 850, 871, 1212, 1246, 1434, 1440
BstMAI GTCTC 3 cut(s) 9, 542, 572
BstMBI GATC 6 cut(s) 847, 868, 1209, 1243, 1431, 1437
BstMWI GCNNNNNNNGC 2 cut(s) 286, 399
BstNI CCWGG 1 cut(s) 722
BstNSI RCATGY 1 cut(s) 1038
BstSCI CCNGG 2 cut(s) 720, 909
BstSFI CTRYAG 5 cut(s) 20, 391, 494, 927, 1253
BstUI CGCG 2 cut(s) 33, 852
BstV1I GCAGC 8 cut(s) 405, 480, 772, 913, 1015, 1018, 1245, 1267
BstV2I GAAGAC 2 cut(s) 749, 981
BstX2I RGATCY 1 cut(s) 1209
BstYI RGATCY 1 cut(s) 1209
Bsu36I CCTNAGG 1 cut(s) 195
BsuRI GGCC 4 cut(s) 219, 332, 442, 456
BtgI CCRYGG 1 cut(s) 451
BtsCI GGATG 5 cut(s) 280, 461, 676, 1154, 1461
BtsIMutI CAGTG 4 cut(s) 220, 265, 633, 1463
Cac8I GCNNGC 1 cut(s) 945
CaiI CAGNNNCTG 3 cut(s) 183, 399, 540
CciI TCATGA 1 cut(s) 967
CfoI GCGC 1 cut(s) 33
Cfr13I GGNCC 2 cut(s) 647, 1082
CseI GACGC 1 cut(s) 385
Csp6I GTAC 4 cut(s) 739, 886, 1197, 1404
CviAII CATG 4 cut(s) 832, 845, 968, 1035
CviQI GTAC 4 cut(s) 739, 886, 1197, 1404
DdeI CTNAG 7 cut(s) 8, 195, 708, 937, 948, 980, 1445
DpnI GATC 6 cut(s) 849, 870, 1211, 1245, 1433, 1439
DpnII GATC 6 cut(s) 847, 868, 1209, 1243, 1431, 1437
DraI TTTAAA 1 cut(s) 858
DrdI GACNNNNNNGTC 1 cut(s) 395
DseDI GACNNNNNNGTC 1 cut(s) 395
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Eco130I CCWWGG 1 cut(s) 560
Eco47I GGWCC 2 cut(s) 647, 1082
Eco57I CTGAAG 1 cut(s) 384
Eco81I CCTNAGG 1 cut(s) 195
EcoNI CCTNNNNNAGG 1 cut(s) 224
EcoRII CCWGG 1 cut(s) 720
EcoT14I CCWWGG 1 cut(s) 560
EcoT22I ATGCAT 1 cut(s) 846
ErhI CCWWGG 1 cut(s) 560
FaeI CATG 4 cut(s) 835, 848, 971, 1038
FalI AAGNNNNNCTT 2 cut(s) 1199, 1231
FaqI GGGAC 2 cut(s) 336, 1023
FatI CATG 4 cut(s) 831, 844, 967, 1034
FauNDI CATATG 1 cut(s) 601
Fnu4HI GCNGC 8 cut(s) 394, 494, 786, 927, 1004, 1032, 1256, 1259
FokI GGATG 4 cut(s) 267, 448, 683, 1161
Fsp4HI GCNGC 8 cut(s) 394, 494, 786, 927, 1004, 1032, 1256, 1259
FspBI CTAG 2 cut(s) 518, 800
GlaI GCGC 1 cut(s) 32
GluI GCNGC 8 cut(s) 394, 494, 786, 927, 1004, 1032, 1256, 1259
HaeIII GGCC 4 cut(s) 219, 332, 442, 456
HapII CCGG 2 cut(s) 425, 910
HgaI GACGC 1 cut(s) 385
HhaI GCGC 1 cut(s) 33
Hin1II CATG 4 cut(s) 835, 848, 971, 1038
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HindIII AAGCTT 1 cut(s) 1175
HinfI GANTC 6 cut(s) 172, 200, 619, 989, 1302, 1310
HpaII CCGG 2 cut(s) 425, 910
HphI GGTGA 4 cut(s) 61, 112, 440, 1441
Hpy166II GTNNAC 3 cut(s) 256, 508, 607
Hpy188I TCNGA 6 cut(s) 466, 536, 685, 1077, 1118, 1309
Hpy8I GTNNAC 3 cut(s) 256, 508, 607
HpyAV CCTTC 8 cut(s) 24, 46, 364, 389, 467, 620, 654, 1355
HpyCH4III ACNGT 9 cut(s) 215, 253, 260, 350, 452, 470, 524, 1047, 1467
HpyCH4IV ACGT 1 cut(s) 628
HpyF10VI GCNNNNNNNGC 2 cut(s) 286, 399
HpyF3I CTNAG 7 cut(s) 8, 195, 708, 937, 948, 980, 1445
HpySE526I ACGT 1 cut(s) 628
Hsp92II CATG 4 cut(s) 835, 848, 971, 1038
HspAI GCGC 1 cut(s) 31
Kpn2I TCCGGA 1 cut(s) 424
Kzo9I GATC 6 cut(s) 847, 868, 1209, 1243, 1431, 1437
LmnI GCTCC 1 cut(s) 724
Lsp1109I GCAGC 8 cut(s) 405, 480, 772, 913, 1015, 1018, 1245, 1267
LweI GCATC 3 cut(s) 289, 661, 831
MaeI CTAG 2 cut(s) 518, 800
MaeII ACGT 1 cut(s) 628
MaeIII GTNAC 4 cut(s) 344, 386, 629, 916
MalI GATC 6 cut(s) 849, 870, 1211, 1245, 1433, 1439
MbiI CCGCTC 1 cut(s) 1080
MboI GATC 6 cut(s) 847, 868, 1209, 1243, 1431, 1437
MboII GAAGA 7 cut(s) 70, 79, 377, 749, 788, 986, 1219
MfeI CAATTG 1 cut(s) 1088
MflI RGATCY 1 cut(s) 1209
MlyI GAGTC 2 cut(s) 998, 1296
MmeI TCCRAC 1 cut(s) 489
Mph1103I ATGCAT 1 cut(s) 846
MroI TCCGGA 1 cut(s) 424
MseI TTAA 7 cut(s) 138, 264, 303, 327, 746, 857, 1133
MspA1I CMGCKG 1 cut(s) 1258
MspI CCGG 2 cut(s) 425, 910
MspR9I CCNGG 2 cut(s) 722, 911
MunI CAATTG 1 cut(s) 1088
Mva1269I GAATGC 3 cut(s) 410, 530, 893
MvaI CCWGG 1 cut(s) 722
MvnI CGCG 2 cut(s) 33, 852
MwoI GCNNNNNNNGC 2 cut(s) 286, 399
NciI CCSGG 1 cut(s) 911
NdeI CATATG 1 cut(s) 601
NdeII GATC 6 cut(s) 847, 868, 1209, 1243, 1431, 1437
NlaIII CATG 4 cut(s) 835, 848, 971, 1038
NlaIV GGNNCC 2 cut(s) 429, 1084
NmuCI GTSAC 3 cut(s) 344, 386, 916
NsiI ATGCAT 1 cut(s) 846
NspI RCATGY 1 cut(s) 1038
PagI TCATGA 1 cut(s) 967
PctI GAATGC 3 cut(s) 410, 530, 893
PfeI GAWTC 4 cut(s) 172, 200, 619, 1310
PflMI CCANNNNNTGG 2 cut(s) 339, 819
PkrI GCNGC 8 cut(s) 395, 495, 787, 928, 1005, 1033, 1257, 1260
PleI GAGTC 2 cut(s) 997, 1296
PpsI GAGTC 2 cut(s) 997, 1296
PshBI ATTAAT 2 cut(s) 746, 1133
Psp1406I AACGTT 1 cut(s) 628
Psp6I CCWGG 1 cut(s) 720
PspGI CCWGG 1 cut(s) 720
PspN4I GGNNCC 2 cut(s) 429, 1084
PspPI GGNCC 2 cut(s) 647, 1082
PstI CTGCAG 4 cut(s) 395, 498, 931, 1257
PstNI CAGNNNCTG 3 cut(s) 183, 399, 540
PsuI RGATCY 1 cut(s) 1209
PvuII CAGCTG 1 cut(s) 1258
RsaI GTAC 4 cut(s) 740, 887, 1198, 1405
RsaNI GTAC 4 cut(s) 739, 886, 1197, 1404
SaqAI TTAA 7 cut(s) 138, 264, 303, 327, 746, 857, 1133
SatI GCNGC 8 cut(s) 394, 494, 786, 927, 1004, 1032, 1256, 1259
Sau3AI GATC 6 cut(s) 847, 868, 1209, 1243, 1431, 1437
Sau96I GGNCC 2 cut(s) 647, 1082
SchI GAGTC 2 cut(s) 998, 1296
ScrFI CCNGG 2 cut(s) 722, 911
SfaNI GCATC 3 cut(s) 289, 661, 831
SfcI CTRYAG 5 cut(s) 20, 391, 494, 927, 1253
SinI GGWCC 2 cut(s) 647, 1082
SsiI CCGC 3 cut(s) 511, 1080, 1427
SspI AATATT 1 cut(s) 701
SspMI CTAG 2 cut(s) 518, 800
StyD4I CCNGG 2 cut(s) 720, 909
StyI CCWWGG 1 cut(s) 560
TaaI ACNGT 9 cut(s) 215, 253, 260, 350, 452, 470, 524, 1047, 1467
TaiI ACGT 1 cut(s) 631
TaqI TCGA 2 cut(s) 319, 1300
TatI WGTACW 3 cut(s) 738, 885, 1403
TfiI GAWTC 4 cut(s) 172, 200, 619, 1310
Tru1I TTAA 7 cut(s) 138, 264, 303, 327, 746, 857, 1133
Tru9I TTAA 7 cut(s) 138, 264, 303, 327, 746, 857, 1133
TscAI CASTG 4 cut(s) 220, 265, 640, 1470
TseFI GTSAC 3 cut(s) 344, 386, 916
TseI GCWGC 8 cut(s) 393, 493, 785, 926, 1003, 1031, 1255, 1258
Tsp45I GTSAC 3 cut(s) 344, 386, 916
TspDTI ATGAA 5 cut(s) 261, 432, 693, 1068, 1284
TspGWI ACGGA 1 cut(s) 300
TspRI CASTG 4 cut(s) 220, 265, 640, 1470
Van91I CCANNNNNTGG 2 cut(s) 339, 819
VpaK11BI GGWCC 2 cut(s) 647, 1082
VspI ATTAAT 2 cut(s) 746, 1133
XagI CCTNNNNNAGG 1 cut(s) 224
XapI RAATTY 4 cut(s) 419, 880, 1101, 1112
XbaI TCTAGA 2 cut(s) 517, 799
XceI RCATGY 1 cut(s) 1038
XspI CTAG 2 cut(s) 518, 800
Zsp2I ATGCAT 1 cut(s) 846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.