Rh5BG318400
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010611
Physical Location & Seq
Forward (+)
42489285 .. 42490923
1639 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG318400.1

Sequence Viewer

Length: 372 bp
ATGGTTTTGGGACGTCGATTATGCTCTCCTAACCACACCGGAAAGGCCCAACAAGCCAACCAAAGTTGCTCTTCCGATTCGGCCGTAAATGATGAAAACCAGGCTCCACTTCCGGCAACCATTTACTACATTTCCTCTGAGAATCTCAAAGATTCCAAGATTCAGGACTGGATTCAAAGGATTGGACTCAACAGCAATTGCAACAGCTGCAAAAACTCATCATGCAATCCAAGCCACCATGTTATAAAAAGACTGTCCTTATGGACTAAGCAAGTAAGAGTTAGCTCTGGGAATGGGATGCCTCTTGGAGATGGCCATATCTGGAGAAAATATGGGCAGAAGGAAATTCTAGGAGCCAAATATCCCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

123

Amino Acids

13.68

Weight (kDa)

9.51

Isoelectric Point (pI)

57.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 104 - 123 9e-06 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 245
AatII GACGTC 1 cut(s) 16
AcoI YGGCCR 2 cut(s) 81, 313
AcsI RAATTY 1 cut(s) 345
AcyI GRCGYC 1 cut(s) 13
AgsI TTSAA 1 cut(s) 176
AjnI CCWGG 2 cut(s) 99, 365
AluBI AGCT 2 cut(s) 207, 285
AluI AGCT 2 cut(s) 207, 285
AoxI GGCC 3 cut(s) 45, 81, 313
ApeKI GCWGC 1 cut(s) 207
ApoI RAATTY 1 cut(s) 345
AspS9I GGNCC 1 cut(s) 46
BalI TGGCCA 1 cut(s) 315
BbvI GCAGC 1 cut(s) 194
BccI CCATC 1 cut(s) 305
BceAI ACGGC 1 cut(s) 68
BciT130I CCWGG 2 cut(s) 101, 367
BfaI CTAG 1 cut(s) 350
BisI GCNGC 1 cut(s) 208
BlsI GCNGC 1 cut(s) 209
Bme1390I CCNGG 2 cut(s) 101, 367
BmgT120I GGNCC 1 cut(s) 46
BmiI GGNNCC 2 cut(s) 105, 355
BmrFI CCNGG 2 cut(s) 101, 367
BmsI GCATC 1 cut(s) 288
BpmI CTGGAG 1 cut(s) 343
BsaHI GRCGYC 1 cut(s) 13
BsaJI CCNNGG 1 cut(s) 365
BsaWI WCCGGW 1 cut(s) 38
Bse1I ACTGG 1 cut(s) 173
BseBI CCWGG 2 cut(s) 101, 367
BseDI CCNNGG 1 cut(s) 365
BseGI GGATG 1 cut(s) 303
BseMII CTCAG 1 cut(s) 129
BseNI ACTGG 1 cut(s) 173
BseX3I CGGCCG 1 cut(s) 81
BseXI GCAGC 1 cut(s) 194
Bsh1285I CGRYCG 1 cut(s) 84
BshFI GGCC 3 cut(s) 47, 83, 315
BsiEI CGRYCG 1 cut(s) 84
BsiSI CCGG 2 cut(s) 39, 113
BslFI GGGAC 1 cut(s) 24
BsmFI GGGAC 1 cut(s) 24
BsnI GGCC 3 cut(s) 47, 83, 315
BspANI GGCC 3 cut(s) 47, 83, 315
BspCNI CTCAG 1 cut(s) 130
BspLI GGNNCC 2 cut(s) 105, 355
BspQI GCTCTTC 1 cut(s) 76
BsrI ACTGG 1 cut(s) 173
BssECI CCNNGG 1 cut(s) 365
BssNI GRCGYC 1 cut(s) 13
Bst2UI CCWGG 2 cut(s) 101, 367
Bst4CI ACNGT 1 cut(s) 255
Bst6I CTCTTC 1 cut(s) 76
BstACI GRCGYC 1 cut(s) 13
BstAPI GCANNNNNTGC 1 cut(s) 207
BstDEI CTNAG 2 cut(s) 138, 267
BstF5I GGATG 1 cut(s) 303
BstMCI CGRYCG 1 cut(s) 84
BstMWI GCNNNNNNNGC 3 cut(s) 53, 207, 231
BstNI CCWGG 2 cut(s) 101, 367
BstSCI CCNGG 2 cut(s) 99, 365
BstV1I GCAGC 1 cut(s) 194
BstZI CGGCCG 1 cut(s) 81
BsuRI GGCC 3 cut(s) 47, 83, 315
BtsCI GGATG 1 cut(s) 303
Cfr13I GGNCC 1 cut(s) 46
CviAII CATG 2 cut(s) 222, 239
CviJI RGCY 9 cut(s) 47, 56, 83, 104, 207, 234, 285, 315, 356
CviKI_1 RGCY 9 cut(s) 47, 56, 83, 104, 207, 234, 285, 315, 356
DdeI CTNAG 2 cut(s) 138, 267
EaeI YGGCCR 2 cut(s) 81, 313
EagI CGGCCG 1 cut(s) 81
Eam1104I CTCTTC 1 cut(s) 76
EarI CTCTTC 1 cut(s) 76
EclXI CGGCCG 1 cut(s) 81
Eco52I CGGCCG 1 cut(s) 81
EcoRII CCWGG 2 cut(s) 99, 365
FaeI CATG 2 cut(s) 225, 242
FaiI YATR 7 cut(s) 22, 223, 240, 245, 262, 318, 333
FalI AAGNNNNNCTT 2 cut(s) 55, 87
FaqI GGGAC 1 cut(s) 24
FatI CATG 2 cut(s) 221, 238
Fnu4HI GCNGC 1 cut(s) 208
FokI GGATG 1 cut(s) 310
Fsp4HI GCNGC 1 cut(s) 208
FspBI CTAG 1 cut(s) 350
GluI GCNGC 1 cut(s) 208
GsuI CTGGAG 1 cut(s) 343
HaeIII GGCC 3 cut(s) 47, 83, 315
HapII CCGG 2 cut(s) 39, 113
Hin1I GRCGYC 1 cut(s) 13
Hin1II CATG 2 cut(s) 225, 242
HinfI GANTC 6 cut(s) 77, 142, 152, 160, 172, 186
HpaII CCGG 2 cut(s) 39, 113
Hpy188I TCNGA 2 cut(s) 76, 139
Hpy188III TCNNGA 2 cut(s) 164, 322
Hpy99I CGWCG 1 cut(s) 18
HpyAV CCTTC 1 cut(s) 334
HpyCH4III ACNGT 1 cut(s) 255
HpyCH4IV ACGT 1 cut(s) 13
HpyCH4V TGCA 3 cut(s) 201, 210, 225
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 207, 231
HpyF3I CTNAG 2 cut(s) 138, 267
HpySE526I ACGT 1 cut(s) 13
Hsp92I GRCGYC 1 cut(s) 13
Hsp92II CATG 2 cut(s) 225, 242
LguI GCTCTTC 1 cut(s) 76
LmnI GCTCC 2 cut(s) 109, 353
LpnPI CCDG 9 cut(s) 52, 86, 113, 126, 149, 154, 273, 307, 352
Lsp1109I GCAGC 1 cut(s) 194
LweI GCATC 1 cut(s) 288
MaeI CTAG 1 cut(s) 350
MaeII ACGT 1 cut(s) 13
MboII GAAGA 1 cut(s) 63
MfeI CAATTG 1 cut(s) 196
MlsI TGGCCA 1 cut(s) 315
MluCI AATT 2 cut(s) 196, 345
MluNI TGGCCA 1 cut(s) 315
MlyI GAGTC 1 cut(s) 180
MnlI CCTC 2 cut(s) 145, 312
Mox20I TGGCCA 1 cut(s) 315
MscI TGGCCA 1 cut(s) 315
Msp20I TGGCCA 1 cut(s) 315
MspA1I CMGCKG 1 cut(s) 207
MspI CCGG 2 cut(s) 39, 113
MspR9I CCNGG 2 cut(s) 101, 367
MunI CAATTG 1 cut(s) 196
MvaI CCWGG 2 cut(s) 101, 367
MwoI GCNNNNNNNGC 3 cut(s) 53, 207, 231
NlaIII CATG 2 cut(s) 225, 242
NlaIV GGNNCC 2 cut(s) 105, 355
PciSI GCTCTTC 1 cut(s) 76
PfeI GAWTC 5 cut(s) 77, 142, 152, 160, 172
PkrI GCNGC 1 cut(s) 209
PleI GAGTC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 180
PsiI TTATAA 1 cut(s) 245
Psp6I CCWGG 2 cut(s) 99, 365
PspGI CCWGG 2 cut(s) 99, 365
PspN4I GGNNCC 2 cut(s) 105, 355
PspPI GGNCC 1 cut(s) 46
PvuII CAGCTG 1 cut(s) 207
SapI GCTCTTC 1 cut(s) 76
SatI GCNGC 1 cut(s) 208
Sau96I GGNCC 1 cut(s) 46
SchI GAGTC 1 cut(s) 180
ScrFI CCNGG 2 cut(s) 101, 367
SetI ASST 4 cut(s) 16, 209, 287, 371
SfaNI GCATC 1 cut(s) 288
Sse9I AATT 2 cut(s) 196, 345
SspMI CTAG 1 cut(s) 350
StyD4I CCNGG 2 cut(s) 99, 365
TaaI ACNGT 1 cut(s) 255
TaiI ACGT 1 cut(s) 16
TaqI TCGA 1 cut(s) 16
TasI AATT 2 cut(s) 196, 345
TfiI GAWTC 5 cut(s) 77, 142, 152, 160, 172
TseI GCWGC 1 cut(s) 207
TspDTI ATGAA 1 cut(s) 108
XapI RAATTY 1 cut(s) 345
XspI CTAG 1 cut(s) 350
ZraI GACGTC 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.