Rh5BG386400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0011623
Physical Location & Seq
Forward (+)
61702765 .. 61703079
315 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG386400.1

Sequence Viewer

Length: 315 bp
ATGGACCAGGTTGTTGGGACAGCACTGAGGTGGCTGGGCGAGGAACTCGTGTTCGCCCATCGTGGTGGCGGGGATCTGCTGGCATGGGGCTTATCGGGTTCAGGCTTGGCTAGTAACAGCCCATTCGATCTGAGAGGGAGTTGGGTATGGCGGAGCATTTCCATAATTCTGTTTCGAAACAGGATCAGGGCCATAGAGGGCAGCGTGGGTGAAAGTGTTTGGCGTCGTGGTGACGAGGGTGGGATCGACCTTCTCCGATGGATTTTGGGTGGGGATATCGGTGCGGCCCCTCGGAGCAGTGGTGTTTGGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

104

Amino Acids

11.23

Weight (kDa)

6.56

Isoelectric Point (pI)

58.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 69, 151, 284
AclWI GGATC 3 cut(s) 81, 191, 251
AcyI GRCGYC 1 cut(s) 223
AjnI CCWGG 1 cut(s) 6
AleI CACNNNNGTG 1 cut(s) 28
AloI GAACNNNNNNTCC 2 cut(s) 35, 67
AlwI GGATC 3 cut(s) 81, 191, 251
AoxI GGCC 2 cut(s) 189, 285
ApeKI GCWGC 1 cut(s) 201
AspS9I GGNCC 3 cut(s) 4, 189, 286
AsuHPI GGTGA 2 cut(s) 221, 242
AsuII TTCGAA 1 cut(s) 175
AvaII GGWCC 1 cut(s) 4
BauI CACGAG 1 cut(s) 47
BbvI GCAGC 1 cut(s) 213
BccI CCATC 2 cut(s) 66, 252
BciT130I CCWGG 1 cut(s) 8
BfaI CTAG 1 cut(s) 111
BisI GCNGC 2 cut(s) 202, 285
BlsI GCNGC 2 cut(s) 203, 286
Bme1390I CCNGG 1 cut(s) 8
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 3 cut(s) 4, 189, 286
BmiI GGNNCC 1 cut(s) 288
BmrFI CCNGG 1 cut(s) 8
Bpu14I TTCGAA 1 cut(s) 175
BsaHI GRCGYC 1 cut(s) 223
BsaJI CCNNGG 1 cut(s) 290
BsaXI ACNNNNNCTCC 1 cut(s) 286
BseBI CCWGG 1 cut(s) 8
BseDI CCNNGG 1 cut(s) 290
BseMII CTCAG 2 cut(s) 17, 122
BseXI GCAGC 1 cut(s) 213
BseYI CCCAGC 1 cut(s) 34
BshFI GGCC 2 cut(s) 191, 287
BslFI GGGAC 1 cut(s) 31
BsmFI GGGAC 1 cut(s) 31
BsnI GGCC 2 cut(s) 191, 287
Bsp119I TTCGAA 1 cut(s) 175
Bsp143I GATC 4 cut(s) 73, 127, 183, 243
BspACI CCGC 3 cut(s) 69, 151, 284
BspANI GGCC 2 cut(s) 191, 287
BspCNI CTCAG 2 cut(s) 18, 123
BspLI GGNNCC 1 cut(s) 288
BspPI GGATC 3 cut(s) 81, 191, 251
BspT104I TTCGAA 1 cut(s) 175
BssECI CCNNGG 1 cut(s) 290
BssMI GATC 4 cut(s) 73, 127, 183, 243
BssNI GRCGYC 1 cut(s) 223
BssSI CACGAG 1 cut(s) 47
Bst2BI CACGAG 1 cut(s) 47
Bst2UI CCWGG 1 cut(s) 8
BstACI GRCGYC 1 cut(s) 223
BstBI TTCGAA 1 cut(s) 175
BstC8I GCNNGC 1 cut(s) 81
BstDEI CTNAG 2 cut(s) 26, 131
BstKTI GATC 4 cut(s) 76, 130, 186, 246
BstMBI GATC 4 cut(s) 73, 127, 183, 243
BstNI CCWGG 1 cut(s) 8
BstSCI CCNGG 1 cut(s) 6
BstV1I GCAGC 1 cut(s) 213
BstX2I RGATCY 1 cut(s) 73
BstXI CCANNNNNNTGG 2 cut(s) 14, 65
BstYI RGATCY 1 cut(s) 73
BsuRI GGCC 2 cut(s) 191, 287
BtsI GCAGTG 1 cut(s) 304
BtsIMutI CAGTG 2 cut(s) 23, 304
Cac8I GCNNGC 1 cut(s) 81
Cfr13I GGNCC 3 cut(s) 4, 189, 286
CseI GACGC 1 cut(s) 212
CsiI ACCWGGT 1 cut(s) 6
CviAII CATG 1 cut(s) 84
CviJI RGCY 7 cut(s) 34, 90, 105, 110, 120, 191, 287
CviKI_1 RGCY 7 cut(s) 34, 90, 105, 110, 120, 191, 287
DdeI CTNAG 2 cut(s) 26, 131
DpnI GATC 4 cut(s) 75, 129, 185, 245
DpnII GATC 4 cut(s) 73, 127, 183, 243
EciI GGCGGA 1 cut(s) 166
Eco32I GATATC 1 cut(s) 277
Eco47I GGWCC 1 cut(s) 4
EcoRII CCWGG 1 cut(s) 6
EcoRV GATATC 1 cut(s) 277
FaeI CATG 1 cut(s) 87
FaiI YATR 4 cut(s) 85, 148, 164, 194
FaqI GGGAC 1 cut(s) 31
FatI CATG 1 cut(s) 83
FauI CCCGC 1 cut(s) 62
Fnu4HI GCNGC 2 cut(s) 202, 285
Fsp4HI GCNGC 2 cut(s) 202, 285
FspBI CTAG 1 cut(s) 111
GluI GCNGC 2 cut(s) 202, 285
GsaI CCCAGC 1 cut(s) 38
HaeIII GGCC 2 cut(s) 191, 287
HgaI GACGC 1 cut(s) 212
Hin1I GRCGYC 1 cut(s) 223
Hin1II CATG 1 cut(s) 87
HphI GGTGA 2 cut(s) 221, 242
Hpy188I TCNGA 3 cut(s) 132, 257, 294
Hpy99I CGWCG 1 cut(s) 228
HpyAV CCTTC 1 cut(s) 260
HpyF3I CTNAG 2 cut(s) 26, 131
Hsp92I GRCGYC 1 cut(s) 223
Hsp92II CATG 1 cut(s) 87
Kzo9I GATC 4 cut(s) 73, 127, 183, 243
LmnI GCTCC 2 cut(s) 153, 294
LpnPI CCDG 6 cut(s) 20, 20, 65, 87, 166, 172
Lsp1109I GCAGC 1 cut(s) 213
MabI ACCWGGT 1 cut(s) 6
MaeI CTAG 1 cut(s) 111
MaeIII GTNAC 2 cut(s) 113, 230
MalI GATC 4 cut(s) 75, 129, 185, 245
MboI GATC 4 cut(s) 73, 127, 183, 243
MflI RGATCY 1 cut(s) 73
MluCI AATT 1 cut(s) 165
MnlI CCTC 6 cut(s) 21, 34, 128, 190, 229, 300
MslI CAYNNNNRTG 2 cut(s) 28, 63
MspR9I CCNGG 1 cut(s) 8
MvaI CCWGG 1 cut(s) 8
NdeII GATC 4 cut(s) 73, 127, 183, 243
NlaIII CATG 1 cut(s) 87
NlaIV GGNNCC 1 cut(s) 288
NmuCI GTSAC 1 cut(s) 230
NspV TTCGAA 1 cut(s) 175
OliI CACNNNNGTG 1 cut(s) 28
PkrI GCNGC 2 cut(s) 203, 286
Psp6I CCWGG 1 cut(s) 6
PspFI CCCAGC 1 cut(s) 34
PspGI CCWGG 1 cut(s) 6
PspN4I GGNNCC 1 cut(s) 288
PspPI GGNCC 3 cut(s) 4, 189, 286
PsuI RGATCY 1 cut(s) 73
RseI CAYNNNNRTG 2 cut(s) 28, 63
SatI GCNGC 2 cut(s) 202, 285
Sau3AI GATC 4 cut(s) 73, 127, 183, 243
Sau96I GGNCC 3 cut(s) 4, 189, 286
ScrFI CCNGG 1 cut(s) 8
SetI ASST 3 cut(s) 12, 32, 252
SexAI ACCWGGT 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 175
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 2 cut(s) 28, 63
Sse9I AATT 1 cut(s) 165
SsiI CCGC 3 cut(s) 69, 151, 284
SspMI CTAG 1 cut(s) 111
StyD4I CCNGG 1 cut(s) 6
TaqI TCGA 3 cut(s) 126, 175, 246
TasI AATT 1 cut(s) 165
TauI GCSGC 1 cut(s) 287
TscAI CASTG 2 cut(s) 30, 304
TseFI GTSAC 1 cut(s) 230
TseI GCWGC 1 cut(s) 201
Tsp45I GTSAC 1 cut(s) 230
TspRI CASTG 2 cut(s) 30, 304
VpaK11BI GGWCC 1 cut(s) 4
XspI CTAG 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.