Rh5DG273000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0011623
Physical Location & Seq
Reverse (-)
33299099 .. 33299308
210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG273000.1

Sequence Viewer

Length: 210 bp
ATGGCGACGAGGTGGTTGGGCGCAGAACTCAAGTTAGACCATCGTGGTGGTTGGGAGCTGCTACCACGGGGTTTGTCGACGTCAGGCTCGGCTAGTCAGAGTCCCTCCGATCTAAGAGGGGACTGGGTGTGGCGGAGCAATTGCGCAGTCCTGGGTCGAACCAAGATCCGCTCCACGGAGGGCGGCGCCTGTGAAGGTGTCTGGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

69

Amino Acids

7.47

Weight (kDa)

8.88

Isoelectric Point (pI)

58.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 83
Acc16I TGCGCA 1 cut(s) 145
AccB1I GGYRCC 1 cut(s) 185
AccBSI CCGCTC 1 cut(s) 171
AccI GTMKAC 1 cut(s) 77
AciI CCGC 3 cut(s) 133, 169, 183
AclWI GGATC 1 cut(s) 160
AcyI GRCGYC 2 cut(s) 80, 186
AfiI CCNNNNNNNGG 1 cut(s) 175
AjnI CCWGG 1 cut(s) 150
AluBI AGCT 1 cut(s) 58
AluI AGCT 1 cut(s) 58
AlwI GGATC 1 cut(s) 160
ApeKI GCWGC 1 cut(s) 58
AspLEI GCGC 3 cut(s) 23, 146, 188
BanI GGYRCC 1 cut(s) 185
BbvI GCAGC 1 cut(s) 45
BccI CCATC 1 cut(s) 48
BciT130I CCWGG 1 cut(s) 152
BfaI CTAG 1 cut(s) 93
BfoI RGCGCY 1 cut(s) 189
BisI GCNGC 2 cut(s) 59, 184
BlsI GCNGC 2 cut(s) 60, 185
Bme1390I CCNGG 1 cut(s) 152
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 1 cut(s) 152
BmrI ACTGGG 1 cut(s) 133
BmuI ACTGGG 1 cut(s) 133
BpuEI CTTGAG 1 cut(s) 14
BsaHI GRCGYC 2 cut(s) 80, 186
BsaJI CCNNGG 3 cut(s) 65, 151, 174
Bsc4I CCNNNNNNNGG 1 cut(s) 175
Bse1I ACTGG 1 cut(s) 128
BseBI CCWGG 1 cut(s) 152
BseDI CCNNGG 3 cut(s) 65, 151, 174
BseLI CCNNNNNNNGG 1 cut(s) 175
BseNI ACTGG 1 cut(s) 128
BseXI GCAGC 1 cut(s) 45
BshNI GGYRCC 1 cut(s) 185
BslFI GGGAC 2 cut(s) 87, 134
BslI CCNNNNNNNGG 1 cut(s) 175
BsmFI GGGAC 2 cut(s) 87, 134
Bsp143I GATC 2 cut(s) 109, 165
BspACI CCGC 3 cut(s) 133, 169, 183
BspLI GGNNCC 1 cut(s) 187
BspPI GGATC 1 cut(s) 160
BspT107I GGYRCC 1 cut(s) 185
BsrBI CCGCTC 1 cut(s) 171
BsrI ACTGG 1 cut(s) 128
BssECI CCNNGG 3 cut(s) 65, 151, 174
BssMI GATC 2 cut(s) 109, 165
BssNI GRCGYC 2 cut(s) 80, 186
Bst2UI CCWGG 1 cut(s) 152
BstACI GRCGYC 2 cut(s) 80, 186
BstDEI CTNAG 1 cut(s) 113
BstDSI CCRYGG 2 cut(s) 65, 174
BstH2I RGCGCY 1 cut(s) 189
BstHHI GCGC 3 cut(s) 23, 146, 188
BstKTI GATC 2 cut(s) 112, 168
BstMBI GATC 2 cut(s) 109, 165
BstNI CCWGG 1 cut(s) 152
BstSCI CCNGG 1 cut(s) 150
BstV1I GCAGC 1 cut(s) 45
BstX2I RGATCY 1 cut(s) 165
BstXI CCANNNNNNTGG 1 cut(s) 47
BstYI RGATCY 1 cut(s) 165
BtgI CCRYGG 2 cut(s) 65, 174
CfoI GCGC 3 cut(s) 23, 146, 188
CspCI CAANNNNNGTGG 2 cut(s) 54, 89
CviJI RGCY 3 cut(s) 58, 87, 92
CviKI_1 RGCY 3 cut(s) 58, 87, 92
DdeI CTNAG 1 cut(s) 113
DinI GGCGCC 1 cut(s) 187
DpnI GATC 2 cut(s) 111, 167
DpnII GATC 2 cut(s) 109, 165
EciI GGCGGA 1 cut(s) 148
EcoRII CCWGG 1 cut(s) 150
EgeI GGCGCC 1 cut(s) 187
EheI GGCGCC 1 cut(s) 187
FaqI GGGAC 2 cut(s) 87, 134
FblI GTMKAC 1 cut(s) 77
Fnu4HI GCNGC 2 cut(s) 59, 184
Fsp4HI GCNGC 2 cut(s) 59, 184
FspBI CTAG 1 cut(s) 93
FspI TGCGCA 1 cut(s) 145
GlaI GCGC 3 cut(s) 22, 145, 187
GluI GCNGC 2 cut(s) 59, 184
HaeII RGCGCY 1 cut(s) 189
HhaI GCGC 3 cut(s) 23, 146, 188
Hin1I GRCGYC 2 cut(s) 80, 186
Hin6I GCGC 3 cut(s) 21, 144, 186
HinP1I GCGC 3 cut(s) 21, 144, 186
HincII GTYRAC 1 cut(s) 78
HindII GTYRAC 1 cut(s) 78
HinfI GANTC 1 cut(s) 100
Hpy166II GTNNAC 1 cut(s) 78
Hpy188I TCNGA 2 cut(s) 99, 109
Hpy8I GTNNAC 1 cut(s) 78
Hpy99I CGWCG 2 cut(s) 10, 82
HpyAV CCTTC 1 cut(s) 188
HpyCH4IV ACGT 1 cut(s) 80
HpyF3I CTNAG 1 cut(s) 113
HpySE526I ACGT 1 cut(s) 80
Hsp92I GRCGYC 2 cut(s) 80, 186
HspAI GCGC 3 cut(s) 21, 144, 186
KasI GGCGCC 1 cut(s) 185
Kzo9I GATC 2 cut(s) 109, 165
LmnI GCTCC 3 cut(s) 55, 135, 176
LpnPI CCDG 6 cut(s) 69, 109, 137, 164, 187, 202
Lsp1109I GCAGC 1 cut(s) 45
MaeI CTAG 1 cut(s) 93
MaeII ACGT 1 cut(s) 80
MalI GATC 2 cut(s) 111, 167
MbiI CCGCTC 1 cut(s) 171
MboI GATC 2 cut(s) 109, 165
MfeI CAATTG 1 cut(s) 139
MflI RGATCY 1 cut(s) 165
MluCI AATT 1 cut(s) 139
Mly113I GGCGCC 1 cut(s) 186
MlyI GAGTC 1 cut(s) 109
MnlI CCTC 4 cut(s) 3, 110, 115, 172
MslI CAYNNNNRTG 1 cut(s) 45
MspR9I CCNGG 1 cut(s) 152
MunI CAATTG 1 cut(s) 139
MvaI CCWGG 1 cut(s) 152
NarI GGCGCC 1 cut(s) 186
NdeII GATC 2 cut(s) 109, 165
NlaIV GGNNCC 1 cut(s) 187
NmeAIII GCCGAG 1 cut(s) 68
NsbI TGCGCA 1 cut(s) 145
PkrI GCNGC 2 cut(s) 60, 185
PleI GAGTC 1 cut(s) 108
PluTI GGCGCC 1 cut(s) 189
PpsI GAGTC 1 cut(s) 108
Psp6I CCWGG 1 cut(s) 150
PspGI CCWGG 1 cut(s) 150
PspN4I GGNNCC 1 cut(s) 187
PsuI RGATCY 1 cut(s) 165
RseI CAYNNNNRTG 1 cut(s) 45
SalI GTCGAC 1 cut(s) 76
SatI GCNGC 2 cut(s) 59, 184
Sau3AI GATC 2 cut(s) 109, 165
SchI GAGTC 1 cut(s) 109
ScrFI CCNGG 1 cut(s) 152
SetI ASST 4 cut(s) 14, 60, 83, 199
SfoI GGCGCC 1 cut(s) 187
SmiMI CAYNNNNRTG 1 cut(s) 45
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 1 cut(s) 139
SsiI CCGC 3 cut(s) 133, 169, 183
SspDI GGCGCC 1 cut(s) 185
SspMI CTAG 1 cut(s) 93
StyD4I CCNGG 1 cut(s) 150
TaiI ACGT 1 cut(s) 83
TaqI TCGA 2 cut(s) 77, 157
TasI AATT 1 cut(s) 139
TauI GCSGC 1 cut(s) 186
TseI GCWGC 1 cut(s) 58
TspGWI ACGGA 1 cut(s) 191
XmiI GTMKAC 1 cut(s) 77
XspI CTAG 1 cut(s) 93
ZraI GACGTC 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.