Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0002327

Physical Location & Seq

Genomic Coordinates Forward (+)

16984899 .. 16989669

Gene Structure 4771 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG162500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2283 bp

ATGTGGAGCTCTTGTGCTCTGGTTGTTACACTACTTGCTTCTCTTCTTCTTCTTCTTCCTCACTTGGCAGCTCTGATCTCTGCTGATCAGGGCTCCACCACTAGTCAGTGGCAAACACTCAAAGGCAACCCGCCTTTGGTCATAGCACGCGGGGGTTTTTCAGGACTATTACCTGGTTCTAGTTCTGATGCATACAAGTTCACATTGTCCACTAGTGTACCAGATGTGTTATTTTGGTGCGATGTCCAACTAACAAAGGAAGGAGCTGGAATTTGCTTTCCAGATCTGCTACTGAATAACAATTCTAATGTTGATATCATCTATCCAAAGAAGGACAAGACTTACATAGTTAACGGAGTTCCTACTGGCGGATGGTTCACCATCGATTATACTCTTAAGGACCTTGCAAATGTTTCTATTACTCAGGGAGTATTTAGTCGAGCTAATAGGTTTGATGACAATTTGTACCCAATTCTCACTGTTGAAGATGTGGCTATGAATTTGACACCACCAGGCTTATGGTTGAATATTGAGAACAATATCTTCTTCTCACAACACAACTTGAGTATGAGAAACTTTGTGCCTTCTGTAATGAGAAAAGTGGTTGTGAATTATATCTCATCACCGGAGGTGGCTTTCCTGAACAGTATTAAAGGAGTTGTTAGTCCAAGAACTACTAAGTTGGTCTTTCGGTTTCTGGAACAAACTGAGATTGAGCCTTCAACCAATCAAACTTATGGATCTCTGTTAAAGAATCTCACATTCATCAAGACATTTGCCTCCGGAATTCTAGTTCCTAAAACTTACATATGGCCAGTCACTAATGACAATTATTTAGAACCACACACTTCACTTGTTTTGGATGCTCATGAAGCAGAACTAATGGTATTTGCGTCCGATTTGGTGAATGATGTGCCATTACCCTACAATTACAGTTATGATCCATTAGCTGAGTGCTTGTCATTCATAGACAATGGCAACTTCACTGTTGATGGTGTGCTGTCTGACTTTCCGATAACTTCATCAGCAGCCATAGATTGCTTTGCTCACCTAAGTCAAAATGCTCCAGTGCAAGACAAGCCTTTGATTATATCGAAAAATGGAGCAAGTGGAGACTACCCTGATTGTACTGATTTGGCATATATGAAAGCACTTGAAGATGGTGTGAATGTACTCGATTGTCCTGTCCAAATGTCCAAGGACGGGATACCATTTTGCTCCAGTTCTATAAACCTTATTCATAGCACAACAGTTGCTCAATCAAGTTTTCAGAACTTAACAATGGTTGTTCCAGAGATTCAGGCTGACAATGGAATATATACCTTTAGCCTGACATGGGAACAGATCAAGACATTACAACCAAGTATATCAAACCCCCAGCAGACTCTGTACAAGTTGTACAGGAATCCAAGGTTCAAAAGTGCTGGGACTTACAAGAGATTATCTGATTTTTTTACCTTGGCAAAGAACAGGAGCTCTGTTTCAGGTGTCATAATCAGCATAGAGTATGCAGCATATCTTGCAGAGAAGCAGGGATTAAGTGTAACAGATGCAGTCATTAATGAGTTGAACAAAGCTGGTTTGGACAATCAGACAGCCCTAGATGTCATGATTCAGTCCACTAATAGTTCTGTTCTAATGAAATTTAAGGAGGAAAGCAAGTGTAAACTTGTTTACAAGATCGAAGAAGTTATTGGTGATGCCCTTAATACAACTCTTCAGGACATCAAGAAGTTTGCCAATTCTGTGGTCATCAACAAGGCCTCTGTCCTTCCTGAAAATAGTGAATACATCACTGGAGCAACCAATGTTGTAACAAAGCTGCATGCATTTGATCTTCCTGTTTATGTCGAACTGTTCAGCAATGAGTATGTATCTCAAGCATGGGACTTCTTCTCTGATCCAACTGTTGAGATCAATTCATTTGTCTCCGGGGCCAATGTTGATGGTGTTATCACAGACTTCCCCAAAACAGCTGCTAGATACAAAAGAAACAGGTGTTTATTTTATAAAGAGAGACCTAGCTACATGAAGCCTGTTCCGCCAGGCGCGCTGTTAACAGTTGTTTCTCCTCGTCAATTGCCACCGGCTGAGGCTCCGAATCCAGTGCTGATAGAGAGTGATGTGATTGAACCACCTTTGCCTTCCGCCTTAGCTCCAGGGATCACCAATGGATCGACAGTAGCACCACCACCACCACCTCCAAACGGGCAGCCTAAGATTGCTGCATGCTTCTTTCTCTCAAATCTGGCTTTGCTTGTTGCCAACCTTTTACTGCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0003674 GO:0003824 GO:0005488 GO:0005509 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0005975 GO:0005976 GO:0006073 GO:0006950 GO:0006952 GO:0006968 GO:0007275 GO:0008081 GO:0008150 GO:0008152 GO:0008889 GO:0009505 GO:0009506 GO:0009653 GO:0009664 GO:0009791 GO:0009826 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010016 GO:0010026 GO:0010052 GO:0010053 GO:0010054 GO:0010103 GO:0010374 GO:0010383 GO:0010441 GO:0010442 GO:0016020 GO:0016043 GO:0016049 GO:0016787 GO:0016788 GO:0021700 GO:0022622 GO:0030054 GO:0030154 GO:0030243 GO:0030312 GO:0031224 GO:0031225 GO:0031226 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0042578 GO:0043167 GO:0043169 GO:0043170 GO:0044036 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044425 GO:0044459 GO:0044464 GO:0045229 GO:0046658 GO:0046872 GO:0048364 GO:0048367 GO:0048468 GO:0048469 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0050896 GO:0051273 GO:0052541 GO:0055044 GO:0060560 GO:0071554 GO:0071555 GO:0071669 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko00564 map00564

Pfam Domains

Protein Families

GDPD

Protein Analysis

760

Amino Acids

83.31

Weight (kDa)

5.0

Isoelectric Point (pI)

40.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GDPD	PF03009	368 - 658	9.8e-26	Glycerophosphoryl diester phosphodiesterase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002327)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66970 AT1G66970 AT1G66970 AT4G26690
fragaria_vesca	FvH4_3g13670 FvH4_3g13670 FvH4_3g13670 FvH4_3g30290 FvH4_3g30290
malus_domestica	MD03G1190400.v1.1 MD11G1207100.v1.1
prunus_persica	Prupe.4G228400_v2.0.a1 Prupe.4G228400_v2.0.a1
pyrus_communis	pycom03g14330 pycom03g14370 pycom11g18010
rosa_chinensis	RchiOBHm_Chr5g0022921 RchiOBHm_Chr5g0056171
rosa_laevigata	RLG00000032682 RLG00000035057 RLG00000035060
rosa_multiflora	Rmu_sc0000680.1_g000027 Rmu_sc0001787.1_g000024 Rmu_sc0002675.1_g000001
rosa_roxburghii	Rroxscaffold_1G00024130 Rroxscaffold_1G00056420
rosa_rugosa	Rorug05G0075500 Rorug05G0297800 Rorug05G0297800
rosa_samantha	Rh5AG162000 Rh5AG368900 Rh5BG161200 Rh5BG379900 Rh5CG175800 Rh5CG402700 Rh5DG162500 Rh5DG392700 Rh5DG392900
rosa_wichuraiana	Rw0G004450 Rw0G012690 Rw5G014580 Rw5G034630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2010
AccII	CGCG	2 cut(s)	150, 2051
AccIII	TCCGGA	1 cut(s)	782
AciI	CCGC	5 cut(s)	131, 150, 369, 2042, 2148
AclWI	GGATC	5 cut(s)	748, 935, 1895, 2171, 2182
AcoI	YGGCCR	1 cut(s)	812
AcsI	RAATTY	4 cut(s)	270, 499, 786, 1643
AcuI	CTGAAG	1 cut(s)	1703
AfaI	GTAC	6 cut(s)	219, 467, 1129, 1173, 1391, 1400
AfiI	CCNNNNNNNGG	5 cut(s)	136, 368, 1203, 1336, 2207
AflII	CTTAAG	1 cut(s)	395
AgsI	TTSAA	7 cut(s)	485, 526, 723, 1157, 1417, 1570, 2132
AhlI	ACTAGT	2 cut(s)	101, 212
AjnI	CCWGG	4 cut(s)	172, 511, 2044, 2158
AjuI	GAANNNNNNNTTGG	2 cut(s)	1677, 1709
AloI	GAACNNNNNNTCC	2 cut(s)	777, 809
Alw21I	GWGCWC	3 cut(s)	11, 19, 1478
Alw26I	GTCTC	3 cut(s)	1107, 1933, 2011
AlwI	GGATC	5 cut(s)	748, 935, 1895, 2171, 2182
AlwNI	CAGNNNCTG	1 cut(s)	1387
Aor13HI	TCCGGA	1 cut(s)	782
AoxI	GGCC	3 cut(s)	812, 1761, 1935
ApeKI	GCWGC	7 cut(s)	68, 1028, 1511, 1822, 1976, 2212, 2225
ApoI	RAATTY	4 cut(s)	270, 499, 786, 1643
AseI	ATTAAT	1 cut(s)	1560
AspLEI	GCGC	2 cut(s)	2051, 2053
AspS9I	GGNCC	2 cut(s)	400, 1935
AsuC2I	CCSGG	1 cut(s)	1933
AsuHPI	GGTGA	6 cut(s)	370, 615, 916, 1040, 1709, 2158
AvaII	GGWCC	1 cut(s)	400
BaeI	ACNNNNGTAYC	2 cut(s)	201, 234
BalI	TGGCCA	1 cut(s)	814
BanII	GRGCYC	3 cut(s)	11, 95, 1478
Bbv12I	GWGCWC	3 cut(s)	11, 19, 1478
BbvCI	CCTCAGC	1 cut(s)	2091
BbvI	GCAGC	7 cut(s)	80, 1040, 1523, 1809, 1963, 2212, 2224
BccI	CCATC	5 cut(s)	366, 389, 986, 1154, 1940
BcgI	CGANNNNNNTGC	2 cut(s)	2089, 2123
BciT130I	CCWGG	4 cut(s)	174, 513, 2046, 2160
BciVI	GTATCC	1 cut(s)	1200
BclI	TGATCA	1 cut(s)	85
BcnI	CCSGG	1 cut(s)	1933
BcoDI	GTCTC	3 cut(s)	1107, 1933, 2011
BcuI	ACTAGT	2 cut(s)	101, 212
BfaI	CTAG	7 cut(s)	102, 180, 213, 791, 1601, 1980, 2022
BfrI	CTTAAG	1 cut(s)	395
BfuI	GTATCC	1 cut(s)	1200
BglII	AGATCT	1 cut(s)	283
BisI	GCNGC	7 cut(s)	69, 1029, 1512, 1823, 1977, 2213, 2226
BlsI	GCNGC	7 cut(s)	70, 1030, 1513, 1824, 1978, 2214, 2227
Bme1390I	CCNGG	5 cut(s)	174, 513, 1933, 2046, 2160
Bme18I	GGWCC	1 cut(s)	400
BmgT120I	GGNCC	2 cut(s)	400, 1935
BmiI	GGNNCC	3 cut(s)	94, 1936, 2097
BmrFI	CCNGG	5 cut(s)	174, 513, 1933, 2046, 2160
BmsI	GCATC	4 cut(s)	178, 853, 1540, 1690
BpmI	CTGGAG	4 cut(s)	1050, 1204, 1818, 2142
Bpu10I	CCTNAGC	2 cut(s)	2091, 2152
BpuEI	CTTGAG	2 cut(s)	583, 1863
BpuMI	CCSGG	1 cut(s)	1933
Bsa29I	ATCGAT	1 cut(s)	384
BsaI	GGTCTC	1 cut(s)	2011
BsaJI	CCNNGG	5 cut(s)	1197, 1409, 1458, 1932, 2159
BsaWI	WCCGGW	2 cut(s)	625, 782
BsaXI	ACNNNNNCTCC	1 cut(s)	28
Bsc4I	CCNNNNNNNGG	5 cut(s)	136, 368, 1203, 1336, 2207
Bse118I	RCCGGY	1 cut(s)	2086
Bse1I	ACTGG	6 cut(s)	370, 815, 1067, 1221, 1801, 2105
Bse3DI	GCAATG	1 cut(s)	1870
BseAI	TCCGGA	1 cut(s)	782
BseBI	CCWGG	4 cut(s)	174, 513, 2046, 2160
BseCI	ATCGAT	1 cut(s)	384
BseDI	CCNNGG	5 cut(s)	1197, 1409, 1458, 1932, 2159
BseGI	GGATG	2 cut(s)	377, 868
BseLI	CCNNNNNNNGG	5 cut(s)	136, 368, 1203, 1336, 2207
BseMI	GCAATG	1 cut(s)	1870
BseMII	CTCAG	4 cut(s)	437, 699, 942, 2082
BseNI	ACTGG	6 cut(s)	370, 815, 1067, 1221, 1801, 2105
BsePI	GCGCGC	1 cut(s)	2049
BseRI	GAGGAG	1 cut(s)	2061
BseXI	GCAGC	7 cut(s)	80, 1040, 1523, 1809, 1963, 2212, 2224
BseYI	CCCAGC	2 cut(s)	1377, 1424
Bsh1236I	CGCG	2 cut(s)	150, 2051
BshFI	GGCC	3 cut(s)	814, 1763, 1937
BshVI	ATCGAT	1 cut(s)	384
BsiHKAI	GWGCWC	3 cut(s)	11, 19, 1478
BsiSI	CCGG	4 cut(s)	626, 783, 1932, 2087
BslFI	GGGAC	2 cut(s)	1441, 1901
BslI	CCNNNNNNNGG	5 cut(s)	136, 368, 1203, 1336, 2207
BsmAI	GTCTC	3 cut(s)	1107, 1933, 2011
BsmFI	GGGAC	2 cut(s)	1441, 1901
BsnI	GGCC	3 cut(s)	814, 1763, 1937
Bso31I	GGTCTC	1 cut(s)	2011
Bsp1286I	GDGCHC	4 cut(s)	11, 19, 95, 1478
Bsp13I	TCCGGA	1 cut(s)	782
Bsp1407I	TGTACA	2 cut(s)	1389, 1398
BspACI	CCGC	5 cut(s)	131, 150, 369, 2042, 2148
BspANI	GGCC	3 cut(s)	814, 1763, 1937
BspCNI	CTCAG	4 cut(s)	436, 700, 943, 2083
BspDI	ATCGAT	1 cut(s)	384
BspEI	TCCGGA	1 cut(s)	782
BspFNI	CGCG	2 cut(s)	150, 2051
BspHI	TCATGA	2 cut(s)	868, 1608
BspLI	GGNNCC	3 cut(s)	94, 1936, 2097
BspPI	GGATC	5 cut(s)	748, 935, 1895, 2171, 2182
BspTI	CTTAAG	1 cut(s)	395
BspTNI	GGTCTC	1 cut(s)	2011
BsrDI	GCAATG	1 cut(s)	1870
BsrFI	RCCGGY	1 cut(s)	2086
BsrGI	TGTACA	2 cut(s)	1389, 1398
BsrI	ACTGG	6 cut(s)	370, 815, 1067, 1221, 1801, 2105
BssAI	RCCGGY	1 cut(s)	2086
BssECI	CCNNGG	5 cut(s)	1197, 1409, 1458, 1932, 2159
BssHII	GCGCGC	1 cut(s)	2049
BssT1I	CCWWGG	3 cut(s)	1197, 1409, 1458
Bst2UI	CCWGG	4 cut(s)	174, 513, 2046, 2160
Bst4CI	ACNGT	9 cut(s)	481, 647, 935, 988, 1252, 1857, 1909, 2062, 2182
Bst6I	CTCTTC	2 cut(s)	48, 1722
BstAFI	CTTAAG	1 cut(s)	395
BstAPI	GCANNNNNTGC	1 cut(s)	1520
BstAUI	TGTACA	2 cut(s)	1389, 1398
BstC8I	GCNNGC	4 cut(s)	148, 1827, 2051, 2230
BstDEI	CTNAG	8 cut(s)	423, 678, 708, 951, 1052, 2091, 2152, 2217
BstF5I	GGATG	2 cut(s)	377, 868
BstFNI	CGCG	2 cut(s)	150, 2051
BstHHI	GCGC	2 cut(s)	2051, 2053
BstMAI	GTCTC	3 cut(s)	1107, 1933, 2011
BstMWI	GCNNNNNNNGC	5 cut(s)	872, 1078, 1520, 2041, 2050
BstNI	CCWGG	4 cut(s)	174, 513, 2046, 2160
BstNSI	RCATGY	2 cut(s)	1829, 2232
BstSCI	CCNGG	5 cut(s)	172, 511, 1931, 2044, 2158
BstUI	CGCG	2 cut(s)	150, 2051
BstV1I	GCAGC	7 cut(s)	80, 1040, 1523, 1809, 1963, 2212, 2224
BstX2I	RGATCY	2 cut(s)	283, 740
BstXI	CCANNNNNNTGG	2 cut(s)	519, 1747
BstYI	RGATCY	2 cut(s)	283, 740
Bsu15I	ATCGAT	1 cut(s)	384
BsuI	GTATCC	1 cut(s)	1200
BsuRI	GGCC	3 cut(s)	814, 1763, 1937
BsuTUI	ATCGAT	1 cut(s)	384
BtgZI	GCGATG	1 cut(s)	255
BtsCI	GGATG	2 cut(s)	377, 868
BtsIMutI	CAGTG	6 cut(s)	113, 477, 984, 1074, 1794, 2112
Cac8I	GCNNGC	4 cut(s)	148, 1827, 2051, 2230
CaiI	CAGNNNCTG	1 cut(s)	1387
CciI	TCATGA	2 cut(s)	868, 1608
CfoI	GCGC	2 cut(s)	2051, 2053
Cfr10I	RCCGGY	1 cut(s)	2086
Cfr13I	GGNCC	2 cut(s)	400, 1935
ClaI	ATCGAT	1 cut(s)	384
CseI	GACGC	1 cut(s)	882
CsiI	ACCWGGT	1 cut(s)	172
Csp6I	GTAC	6 cut(s)	218, 466, 1128, 1172, 1390, 1399
CviAII	CATG	7 cut(s)	869, 1335, 1609, 1826, 1884, 2029, 2229
CviQI	GTAC	6 cut(s)	218, 466, 1128, 1172, 1390, 1399
DdeI	CTNAG	8 cut(s)	423, 678, 708, 951, 1052, 2091, 2152, 2217
EaeI	YGGCCR	1 cut(s)	812
Eam1104I	CTCTTC	2 cut(s)	48, 1722
EarI	CTCTTC	2 cut(s)	48, 1722
EciI	GGCGGA	3 cut(s)	384, 2031, 2137
Ecl136II	GAGCTC	2 cut(s)	9, 1476
Eco130I	CCWWGG	3 cut(s)	1197, 1409, 1458
Eco147I	AGGCCT	1 cut(s)	1763
Eco24I	GRGCYC	3 cut(s)	11, 95, 1478
Eco31I	GGTCTC	1 cut(s)	2011
Eco32I	GATATC	1 cut(s)	316
Eco47I	GGWCC	1 cut(s)	400
Eco53kI	GAGCTC	2 cut(s)	9, 1476
Eco57I	CTGAAG	1 cut(s)	1703
EcoICRI	GAGCTC	2 cut(s)	9, 1476
EcoO109I	RGGNCCY	1 cut(s)	400
EcoRI	GAATTC	1 cut(s)	786
EcoRII	CCWGG	4 cut(s)	172, 511, 2044, 2158
EcoRV	GATATC	1 cut(s)	316
EcoT14I	CCWWGG	3 cut(s)	1197, 1409, 1458
EcoT22I	ATGCAT	2 cut(s)	193, 1831
EcoT38I	GRGCYC	3 cut(s)	11, 95, 1478
ErhI	CCWWGG	3 cut(s)	1197, 1409, 1458
FaeI	CATG	7 cut(s)	872, 1338, 1612, 1829, 1887, 2032, 2232
FalI	AAGNNNNNCTT	2 cut(s)	671, 703
FaqI	GGGAC	2 cut(s)	1441, 1901
FatI	CATG	7 cut(s)	868, 1334, 1608, 1825, 1883, 2028, 2228
FauI	CCCGC	2 cut(s)	138, 143
FauNDI	CATATG	1 cut(s)	809
FbaI	TGATCA	1 cut(s)	85
Fnu4HI	GCNGC	7 cut(s)	69, 1029, 1512, 1823, 1977, 2213, 2226
FokI	GGATG	2 cut(s)	384, 875
FriOI	GRGCYC	3 cut(s)	11, 95, 1478
Fsp4HI	GCNGC	7 cut(s)	69, 1029, 1512, 1823, 1977, 2213, 2226
FspBI	CTAG	7 cut(s)	102, 180, 213, 791, 1601, 1980, 2022
GlaI	GCGC	2 cut(s)	2050, 2052
GluI	GCNGC	7 cut(s)	69, 1029, 1512, 1823, 1977, 2213, 2226
GsaI	CCCAGC	2 cut(s)	1381, 1428
GsuI	CTGGAG	4 cut(s)	1050, 1204, 1818, 2142
HaeIII	GGCC	3 cut(s)	814, 1763, 1937
HapII	CCGG	4 cut(s)	626, 783, 1932, 2087
HgaI	GACGC	1 cut(s)	882
HhaI	GCGC	2 cut(s)	2051, 2053
Hin1II	CATG	7 cut(s)	872, 1338, 1612, 1829, 1887, 2032, 2232
Hin6I	GCGC	2 cut(s)	2049, 2051
HinP1I	GCGC	2 cut(s)	2049, 2051
HincII	GTYRAC	2 cut(s)	352, 2058
HindII	GTYRAC	2 cut(s)	352, 2058
HinfI	GANTC	6 cut(s)	754, 1297, 1384, 1405, 1612, 2101
HpaI	GTTAAC	2 cut(s)	352, 2058
HpaII	CCGG	4 cut(s)	626, 783, 1932, 2087
HphI	GGTGA	6 cut(s)	370, 615, 916, 1040, 1709, 2158
Hpy166II	GTNNAC	9 cut(s)	201, 210, 218, 352, 378, 1620, 1667, 1675, 2058
Hpy8I	GTNNAC	9 cut(s)	201, 210, 218, 352, 378, 1620, 1667, 1675, 2058
HpyAV	CCTTC	6 cut(s)	254, 325, 594, 729, 1781, 2154
HpyCH4III	ACNGT	9 cut(s)	481, 647, 935, 988, 1252, 1857, 1909, 2062, 2182
HpyCH4V	TGCA	9 cut(s)	191, 407, 1072, 1511, 1523, 1553, 1825, 1829, 2228
HpyF10VI	GCNNNNNNNGC	5 cut(s)	872, 1078, 1520, 2041, 2050
HpyF3I	CTNAG	8 cut(s)	423, 678, 708, 951, 1052, 2091, 2152, 2217
Hsp92II	CATG	7 cut(s)	872, 1338, 1612, 1829, 1887, 2032, 2232
HspAI	GCGC	2 cut(s)	2049, 2051
Kpn2I	TCCGGA	1 cut(s)	782
Ksp22I	TGATCA	1 cut(s)	85
KspAI	GTTAAC	2 cut(s)	352, 2058
Lsp1109I	GCAGC	7 cut(s)	80, 1040, 1523, 1809, 1963, 2212, 2224
LweI	GCATC	4 cut(s)	178, 853, 1540, 1690
MabI	ACCWGGT	1 cut(s)	172
MaeI	CTAG	7 cut(s)	102, 180, 213, 791, 1601, 1980, 2022
MaeIII	GTNAC	4 cut(s)	25, 817, 1543, 1813
MfeI	CAATTG	1 cut(s)	2078
MflI	RGATCY	2 cut(s)	283, 740
MhlI	GDGCHC	4 cut(s)	11, 19, 95, 1478
MlsI	TGGCCA	1 cut(s)	814
MluNI	TGGCCA	1 cut(s)	814
MlyI	GAGTC	1 cut(s)	1378
MmeI	TCCRAC	2 cut(s)	271, 1928
MnlI	CCTC	8 cut(s)	69, 622, 790, 1645, 1774, 2082, 2086, 2211
Mox20I	TGGCCA	1 cut(s)	814
Mph1103I	ATGCAT	2 cut(s)	193, 1831
MroI	TCCGGA	1 cut(s)	782
MscI	TGGCCA	1 cut(s)	814
Msp20I	TGGCCA	1 cut(s)	814
MspA1I	CMGCKG	1 cut(s)	1976
MspCI	CTTAAG	1 cut(s)	395
MspI	CCGG	4 cut(s)	626, 783, 1932, 2087
MspR9I	CCNGG	5 cut(s)	174, 513, 1933, 2046, 2160
MunI	CAATTG	1 cut(s)	2078
MvaI	CCWGG	4 cut(s)	174, 513, 2046, 2160
MvnI	CGCG	2 cut(s)	150, 2051
MwoI	GCNNNNNNNGC	5 cut(s)	872, 1078, 1520, 2041, 2050
NciI	CCSGG	1 cut(s)	1933
NdeI	CATATG	1 cut(s)	809
NlaIII	CATG	7 cut(s)	872, 1338, 1612, 1829, 1887, 2032, 2232
NlaIV	GGNNCC	3 cut(s)	94, 1936, 2097
NmuCI	GTSAC	1 cut(s)	817
NsiI	ATGCAT	2 cut(s)	193, 1831
NspI	RCATGY	2 cut(s)	1829, 2232
PaeI	GCATGC	2 cut(s)	1829, 2232
PagI	TCATGA	2 cut(s)	868, 1608
PauI	GCGCGC	1 cut(s)	2049
PceI	AGGCCT	1 cut(s)	1763
PfeI	GAWTC	5 cut(s)	754, 1297, 1405, 1612, 2101
PkrI	GCNGC	7 cut(s)	70, 1030, 1513, 1824, 1978, 2214, 2227
PleI	GAGTC	1 cut(s)	1378
PpsI	GAGTC	1 cut(s)	1378
PpuMI	RGGWCCY	1 cut(s)	400
PshBI	ATTAAT	1 cut(s)	1560
PsiI	TTATAA	1 cut(s)	2010
Psp124BI	GAGCTC	2 cut(s)	11, 1478
Psp5II	RGGWCCY	1 cut(s)	400
Psp6I	CCWGG	4 cut(s)	172, 511, 2044, 2158
PspFI	CCCAGC	2 cut(s)	1377, 1424
PspGI	CCWGG	4 cut(s)	172, 511, 2044, 2158
PspN4I	GGNNCC	3 cut(s)	94, 1936, 2097
PspPI	GGNCC	2 cut(s)	400, 1935
PspPPI	RGGWCCY	1 cut(s)	400
PstNI	CAGNNNCTG	1 cut(s)	1387
PsuI	RGATCY	2 cut(s)	283, 740
PteI	GCGCGC	1 cut(s)	2049
PvuII	CAGCTG	1 cut(s)	1976
RsaI	GTAC	6 cut(s)	219, 467, 1129, 1173, 1391, 1400
RsaNI	GTAC	6 cut(s)	218, 466, 1128, 1172, 1390, 1399
SacI	GAGCTC	2 cut(s)	11, 1478
SatI	GCNGC	7 cut(s)	69, 1029, 1512, 1823, 1977, 2213, 2226
Sau96I	GGNCC	2 cut(s)	400, 1935
SchI	GAGTC	1 cut(s)	1378
ScrFI	CCNGG	5 cut(s)	174, 513, 1933, 2046, 2160
SduI	GDGCHC	4 cut(s)	11, 19, 95, 1478
SexAI	ACCWGGT	1 cut(s)	172
SfaNI	GCATC	4 cut(s)	178, 853, 1540, 1690
SinI	GGWCC	1 cut(s)	400
SmlI	CTYRAG	3 cut(s)	395, 562, 1878
SmoI	CTYRAG	3 cut(s)	395, 562, 1878
SpeI	ACTAGT	2 cut(s)	101, 212
SphI	GCATGC	2 cut(s)	1829, 2232
SseBI	AGGCCT	1 cut(s)	1763
SsiI	CCGC	5 cut(s)	131, 150, 369, 2042, 2148
SspI	AATATT	1 cut(s)	529
SspMI	CTAG	7 cut(s)	102, 180, 213, 791, 1601, 1980, 2022
SstI	GAGCTC	2 cut(s)	11, 1478
StuI	AGGCCT	1 cut(s)	1763
StyD4I	CCNGG	5 cut(s)	172, 511, 1931, 2044, 2158
StyI	CCWWGG	3 cut(s)	1197, 1409, 1458
TaaI	ACNGT	9 cut(s)	481, 647, 935, 988, 1252, 1857, 1909, 2062, 2182
TaqI	TCGA	7 cut(s)	384, 439, 1094, 1176, 1683, 1851, 2177
TatI	WGTACW	4 cut(s)	1127, 1171, 1389, 1398
TfiI	GAWTC	5 cut(s)	754, 1297, 1405, 1612, 2101
TscAI	CASTG	6 cut(s)	113, 484, 991, 1074, 1801, 2112
TseFI	GTSAC	1 cut(s)	817
TseI	GCWGC	7 cut(s)	68, 1028, 1511, 1822, 1976, 2212, 2225
Tsp45I	GTSAC	1 cut(s)	817
TspGWI	ACGGA	1 cut(s)	369
TspRI	CASTG	6 cut(s)	113, 484, 991, 1074, 1801, 2112
Vha464I	CTTAAG	1 cut(s)	395
VpaK11BI	GGWCC	1 cut(s)	400
VspI	ATTAAT	1 cut(s)	1560
XapI	RAATTY	4 cut(s)	270, 499, 786, 1643
XceI	RCATGY	2 cut(s)	1829, 2232
XcmI	CCANNNNNNNNNTGG	1 cut(s)	516
XspI	CTAG	7 cut(s)	102, 180, 213, 791, 1601, 1980, 2022
Zsp2I	ATGCAT	2 cut(s)	193, 1831

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.