Rh6BG063500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0009729
Physical Location & Seq
Reverse (-)
9884277 .. 9893679
9403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG063500.1

Sequence Viewer

Length: 663 bp
ATGAGTGGGACAGGGGACCTTGACAAGGATCCAGAGGGAATGACTTTAGGCCCTGTTAAGGAGCTCATGGAGGGCTCGGTCATGTGGACCAACCTCTCTGGGTTTTCAGAAACAGAGCAACTAGTTCTAGTTCTAAGCCGCAAACCTGTTCTCTCTCTCCAACCTAAGACCAAGCTCTCTCCTTCACTCCTTGTGGAAGATCCTTGGTCACCCAGAAGCGCGGCTGCTCAAGTTTTCGTTATGAATATTCTCAAACTTTTGTCCGGCTCTCTGTTTCTGGAGAAAGATGACCAAAAGTTTGATGATATTGGAGATGATTGTGGGAGTCTTGATCTGTTTCCATTTGGATTTCCCAGATCATTAACTGTGGATAGGATTCAGGTTCAAAGCACTTGTCCTGCTCTGTTCCTTCACATGATCGACATCGATGGAGAATTCAAACGATACCTATTCAATATCAATGGAATACTGAATTCTCAAAAGGATTGCGGTACTTCCCTGCCATGCCCTGCTGAAGTAACTATCCTAAGCAGCAGAGCCCTGCAGAGATTGAGATTGATGGAGAATTCAAACGATACCTGTTCTTTCCTGCCATGCCTTGCTCAAGTAACTGTCATAAACAGCAGAGAGATTCATTCAGAGCCCTGCAGAGATCGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

220

Amino Acids

24.42

Weight (kDa)

4.77

Isoelectric Point (pI)

45.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 221
AciI CCGC 3 cut(s) 139, 221, 489
AclWI GGATC 3 cut(s) 23, 36, 194
AcsI RAATTY 3 cut(s) 434, 472, 565
AcuI CTGAAG 1 cut(s) 534
AfaI GTAC 1 cut(s) 493
AfiI CCNNNNNNNGG 2 cut(s) 25, 58
AgsI TTSAA 4 cut(s) 386, 439, 454, 570
AhlI ACTAGT 1 cut(s) 121
AluBI AGCT 2 cut(s) 64, 175
AluI AGCT 2 cut(s) 64, 175
Alw21I GWGCWC 1 cut(s) 66
AlwI GGATC 3 cut(s) 23, 36, 194
AoxI GGCC 1 cut(s) 49
ApeKI GCWGC 2 cut(s) 224, 531
ApoI RAATTY 3 cut(s) 434, 472, 565
ArsI GACNNNNNNTTYG 4 cut(s) 281, 313, 379, 411
AspLEI GCGC 1 cut(s) 221
AspS9I GGNCC 3 cut(s) 16, 50, 87
AsuHPI GGTGA 1 cut(s) 201
AvaII GGWCC 2 cut(s) 16, 87
BamHI GGATCC 1 cut(s) 28
BanII GRGCYC 4 cut(s) 66, 77, 541, 645
Bbv12I GWGCWC 1 cut(s) 66
BbvI GCAGC 2 cut(s) 211, 543
BccI CCATC 2 cut(s) 422, 553
BcuI ACTAGT 1 cut(s) 121
BfaI CTAG 2 cut(s) 122, 128
BfmI CTRYAG 2 cut(s) 542, 646
BisI GCNGC 4 cut(s) 139, 222, 225, 532
BlsI GCNGC 4 cut(s) 140, 223, 226, 533
Bme18I GGWCC 2 cut(s) 16, 87
BmgT120I GGNCC 3 cut(s) 16, 50, 87
BmiI GGNNCC 2 cut(s) 17, 30
BpmI CTGGAG 1 cut(s) 299
Bpu10I CCTNAGC 1 cut(s) 527
BpuEI CTTGAG 2 cut(s) 213, 588
Bsa29I ATCGAT 1 cut(s) 426
BsaBI GATNNNNATC 1 cut(s) 422
BsaJI CCNNGG 1 cut(s) 203
Bsc4I CCNNNNNNNGG 2 cut(s) 25, 58
Bse8I GATNNNNATC 1 cut(s) 422
BseCI ATCGAT 1 cut(s) 426
BseDI CCNNGG 1 cut(s) 203
BseJI GATNNNNATC 1 cut(s) 422
BseLI CCNNNNNNNGG 2 cut(s) 25, 58
BseXI GCAGC 2 cut(s) 211, 543
Bsh1236I CGCG 1 cut(s) 221
BshFI GGCC 1 cut(s) 51
BshVI ATCGAT 1 cut(s) 426
BsiHKAI GWGCWC 1 cut(s) 66
BsiSI CCGG 1 cut(s) 264
BslFI GGGAC 2 cut(s) 22, 29
BslI CCNNNNNNNGG 2 cut(s) 25, 58
BsmFI GGGAC 2 cut(s) 22, 29
BsnI GGCC 1 cut(s) 51
Bsp1286I GDGCHC 4 cut(s) 66, 77, 541, 645
Bsp143I GATC 6 cut(s) 28, 199, 331, 356, 417, 652
BspACI CCGC 3 cut(s) 139, 221, 489
BspANI GGCC 1 cut(s) 51
BspDI ATCGAT 1 cut(s) 426
BspFNI CGCG 1 cut(s) 221
BspLI GGNNCC 2 cut(s) 17, 30
BspMAI CTGCAG 2 cut(s) 546, 650
BspPI GGATC 3 cut(s) 23, 36, 194
BssECI CCNNGG 1 cut(s) 203
BssMI GATC 6 cut(s) 28, 199, 331, 356, 417, 652
BssT1I CCWWGG 1 cut(s) 203
Bst4CI ACNGT 2 cut(s) 367, 613
BstDEI CTNAG 3 cut(s) 134, 165, 527
BstEII GGTNACC 1 cut(s) 207
BstENI CCTNNNNNAGG 1 cut(s) 23
BstFNI CGCG 1 cut(s) 221
BstHHI GCGC 1 cut(s) 221
BstKTI GATC 6 cut(s) 31, 202, 334, 359, 420, 655
BstMBI GATC 6 cut(s) 28, 199, 331, 356, 417, 652
BstPI GGTNACC 1 cut(s) 207
BstSFI CTRYAG 2 cut(s) 542, 646
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 2 cut(s) 211, 543
BstX2I RGATCY 2 cut(s) 28, 199
BstYI RGATCY 2 cut(s) 28, 199
Bsu15I ATCGAT 1 cut(s) 426
BsuRI GGCC 1 cut(s) 51
BsuTUI ATCGAT 1 cut(s) 426
CfoI GCGC 1 cut(s) 221
Cfr13I GGNCC 3 cut(s) 16, 50, 87
ClaI ATCGAT 1 cut(s) 426
Csp6I GTAC 1 cut(s) 492
CviAII CATG 5 cut(s) 67, 82, 415, 504, 594
CviJI RGCY 9 cut(s) 51, 64, 75, 138, 175, 224, 267, 539, 643
CviKI_1 RGCY 9 cut(s) 51, 64, 75, 138, 175, 224, 267, 539, 643
CviQI GTAC 1 cut(s) 492
DdeI CTNAG 3 cut(s) 134, 165, 527
DpnI GATC 6 cut(s) 30, 201, 333, 358, 419, 654
DpnII GATC 6 cut(s) 28, 199, 331, 356, 417, 652
Ecl136II GAGCTC 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 203
Eco24I GRGCYC 4 cut(s) 66, 77, 541, 645
Eco47I GGWCC 2 cut(s) 16, 87
Eco53kI GAGCTC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 534
Eco91I GGTNACC 1 cut(s) 207
EcoICRI GAGCTC 1 cut(s) 64
EcoNI CCTNNNNNAGG 1 cut(s) 23
EcoO109I RGGNCCY 2 cut(s) 16, 50
EcoO65I GGTNACC 1 cut(s) 207
EcoRI GAATTC 3 cut(s) 434, 472, 565
EcoT14I CCWWGG 1 cut(s) 203
EcoT38I GRGCYC 4 cut(s) 66, 77, 541, 645
ErhI CCWWGG 1 cut(s) 203
FaeI CATG 5 cut(s) 70, 85, 418, 507, 597
FaiI YATR 7 cut(s) 68, 83, 242, 416, 505, 595, 617
FaqI GGGAC 2 cut(s) 22, 29
FatI CATG 5 cut(s) 66, 81, 414, 503, 593
Fnu4HI GCNGC 4 cut(s) 139, 222, 225, 532
FriOI GRGCYC 4 cut(s) 66, 77, 541, 645
Fsp4HI GCNGC 4 cut(s) 139, 222, 225, 532
FspBI CTAG 2 cut(s) 122, 128
GlaI GCGC 1 cut(s) 220
GluI GCNGC 4 cut(s) 139, 222, 225, 532
GsuI CTGGAG 1 cut(s) 299
HaeIII GGCC 1 cut(s) 51
HapII CCGG 1 cut(s) 264
HhaI GCGC 1 cut(s) 221
Hin1II CATG 5 cut(s) 70, 85, 418, 507, 597
Hin6I GCGC 1 cut(s) 219
HinP1I GCGC 1 cut(s) 219
HinfI GANTC 3 cut(s) 325, 376, 631
HpaII CCGG 1 cut(s) 264
HphI GGTGA 1 cut(s) 201
Hpy166II GTNNAC 1 cut(s) 87
Hpy188I TCNGA 2 cut(s) 109, 640
Hpy188III TCNNGA 4 cut(s) 32, 278, 329, 656
Hpy8I GTNNAC 1 cut(s) 87
HpyAV CCTTC 2 cut(s) 192, 419
HpyCH4III ACNGT 2 cut(s) 367, 613
HpyCH4V TGCA 2 cut(s) 544, 648
HpyF3I CTNAG 3 cut(s) 134, 165, 527
Hsp92II CATG 5 cut(s) 70, 85, 418, 507, 597
HspAI GCGC 1 cut(s) 219
Kzo9I GATC 6 cut(s) 28, 199, 331, 356, 417, 652
LmnI GCTCC 1 cut(s) 61
Lsp1109I GCAGC 2 cut(s) 211, 543
MaeI CTAG 2 cut(s) 122, 128
MaeIII GTNAC 3 cut(s) 207, 517, 607
MalI GATC 6 cut(s) 30, 201, 333, 358, 419, 654
MboI GATC 6 cut(s) 28, 199, 331, 356, 417, 652
MboII GAAGA 1 cut(s) 209
MflI RGATCY 2 cut(s) 28, 199
MhlI GDGCHC 4 cut(s) 66, 77, 541, 645
MluCI AATT 3 cut(s) 434, 472, 565
MlyI GAGTC 1 cut(s) 334
MmeI TCCRAC 1 cut(s) 184
MnlI CCTC 3 cut(s) 28, 64, 104
MseI TTAA 2 cut(s) 57, 362
MspI CCGG 1 cut(s) 264
MvnI CGCG 1 cut(s) 221
NdeII GATC 6 cut(s) 28, 199, 331, 356, 417, 652
NlaIII CATG 5 cut(s) 70, 85, 418, 507, 597
NlaIV GGNNCC 2 cut(s) 17, 30
NmuCI GTSAC 1 cut(s) 207
PfeI GAWTC 2 cut(s) 376, 631
PkrI GCNGC 4 cut(s) 140, 223, 226, 533
PleI GAGTC 1 cut(s) 333
PpsI GAGTC 1 cut(s) 333
PpuMI RGGWCCY 1 cut(s) 16
Psp124BI GAGCTC 1 cut(s) 66
Psp5II RGGWCCY 1 cut(s) 16
PspEI GGTNACC 1 cut(s) 207
PspN4I GGNNCC 2 cut(s) 17, 30
PspPI GGNCC 3 cut(s) 16, 50, 87
PspPPI RGGWCCY 1 cut(s) 16
PstI CTGCAG 2 cut(s) 546, 650
PsuI RGATCY 2 cut(s) 28, 199
RsaI GTAC 1 cut(s) 493
RsaNI GTAC 1 cut(s) 492
SacI GAGCTC 1 cut(s) 66
SaqAI TTAA 2 cut(s) 57, 362
SatI GCNGC 4 cut(s) 139, 222, 225, 532
Sau3AI GATC 6 cut(s) 28, 199, 331, 356, 417, 652
Sau96I GGNCC 3 cut(s) 16, 50, 87
SchI GAGTC 1 cut(s) 334
SduI GDGCHC 4 cut(s) 66, 77, 541, 645
SetI ASST 9 cut(s) 21, 66, 96, 148, 166, 177, 384, 450, 581
SfcI CTRYAG 2 cut(s) 542, 646
SinI GGWCC 2 cut(s) 16, 87
SmlI CTYRAG 2 cut(s) 228, 603
SmoI CTYRAG 2 cut(s) 228, 603
SpeI ACTAGT 1 cut(s) 121
Sse9I AATT 3 cut(s) 434, 472, 565
SsiI CCGC 3 cut(s) 139, 221, 489
SspI AATATT 1 cut(s) 247
SspMI CTAG 2 cut(s) 122, 128
SstI GAGCTC 1 cut(s) 66
StyI CCWWGG 1 cut(s) 203
TaaI ACNGT 2 cut(s) 367, 613
TaqI TCGA 3 cut(s) 420, 426, 655
TaqII GACCGA 1 cut(s) 67
TasI AATT 3 cut(s) 434, 472, 565
TauI GCSGC 2 cut(s) 141, 224
TfiI GAWTC 2 cut(s) 376, 631
Tru1I TTAA 2 cut(s) 57, 362
Tru9I TTAA 2 cut(s) 57, 362
TseFI GTSAC 1 cut(s) 207
TseI GCWGC 2 cut(s) 224, 531
Tsp45I GTSAC 1 cut(s) 207
TspDTI ATGAA 2 cut(s) 257, 623
VpaK11BI GGWCC 2 cut(s) 16, 87
XagI CCTNNNNNAGG 1 cut(s) 23
XapI RAATTY 3 cut(s) 434, 472, 565
XspI CTAG 2 cut(s) 122, 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.