Rw2G010480

Protein transport protein Sec24-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0009667
Physical Location & Seq
Forward (+)
10632293 .. 10637346
5054 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G010480.1

Sequence Viewer

Length: 963 bp
ATGGGTATTGGTTTAGAATATGAGTACTGGATTTCTTCAGTACCCATAGGAATTCGACGATGGGTACTAGACTTCAATTACTTTTGTTCTAAACCGCGATTTCTCGTTCTCTGTACCATCAATTTGGCTCTCCCTTGGCTCCAGTATGTAAAATCACTCCTTCTACTCCTTATCTTTTCTTTAAATATTTCTTTTGACAGAGTAAGTACACAGCCCTTGTTACAATTCTTGGCTCCAGTGGAAACTTGTAAGGACTGTATTGGATCAGCACTTGAAACAATTAGACCAACAACTTCATGGGAGAGAACAACAGCAGTAGGGCAAGGAGTAGATGGTGTTTTGATGGGTGGGCGAGGTTTTGAGGTGGCAATTGAAGCCCTTTTAAACTACCTTGGATCTGAATATGGAAGCACATTTGCACTAGGTAGAGTCTTTGCTTTTTTGTCTGGTCCTCTTGACTATGGAGCTGGGCAACTAGATACAAGACGGTACGATGAACAATATGCTAGTAGAGGAGAGGATGCGGACCGTGCATTGCTTCCTGAGCAGACACCTTTCTATAAAGATTTGGCTGTTGTCGCTGTTCAAGCAGGTGTTTGCGTAGACATCTTTGCTGTTACAAATGAGTATACAGATTTGGCGTCTCTGAAGTTTCTTAGTGTAGAAAGTGGAGGCTCATTGTACTTTTATTCAAATACTGATGATTCAACACTTCCACAGGACATGTACCGGATGCTAAGTCGACCATATGCTTTTGGCTGTGTACTGCGATTAAGGACATCATCTGAATTCAAAACTGGCCATTCTCATGGTCACTTCTTTCCAGATCCACAGTATGAGAATGTTCAACACATTATCTGTTGTGATTCTTACGCCACATATGCTTATGACTTTGAGTTTGAAGCTACCAAGGAATTTGCAAAGTATGTAGCTCATATTGCCGTCATGGTATCTTCCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

320

Amino Acids

35.96

Weight (kDa)

4.87

Isoelectric Point (pI)

39.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec23_trunk PF04811 75 - 245 4.6e-20 Sec23/Sec24 trunk domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 581
Acc36I ACCTGC 1 cut(s) 581
AccI GTMKAC 3 cut(s) 603, 629, 742
AccII CGCG 1 cut(s) 97
AciI CCGC 2 cut(s) 95, 524
AclWI GGATC 3 cut(s) 271, 403, 821
AcoI YGGCCR 1 cut(s) 799
AcsI RAATTY 3 cut(s) 51, 788, 914
AcuI CTGAAG 2 cut(s) 21, 668
AcyI GRCGYC 1 cut(s) 641
AfaI GTAC 9 cut(s) 26, 42, 66, 115, 208, 491, 683, 728, 765
AflIII ACRYGT 1 cut(s) 723
AgsI TTSAA 9 cut(s) 76, 275, 374, 587, 693, 708, 793, 848, 902
AluBI AGCT 3 cut(s) 467, 905, 932
AluI AGCT 3 cut(s) 467, 905, 932
Alw26I GTCTC 1 cut(s) 648
AlwI GGATC 3 cut(s) 271, 403, 821
AoxI GGCC 1 cut(s) 799
ApoI RAATTY 3 cut(s) 51, 788, 914
ArsI GACNNNNNNTTYG 2 cut(s) 881, 913
AspS9I GGNCC 2 cut(s) 449, 526
AvaII GGWCC 2 cut(s) 449, 526
BalI TGGCCA 1 cut(s) 801
BccI CCATC 4 cut(s) 54, 125, 326, 337
BceAI ACGGC 1 cut(s) 926
BcoDI GTCTC 1 cut(s) 648
BfaI CTAG 4 cut(s) 68, 422, 476, 507
BfuAI ACCTGC 1 cut(s) 581
BmcAI AGTACT 1 cut(s) 26
Bme18I GGWCC 2 cut(s) 449, 526
BmgT120I GGNCC 2 cut(s) 449, 526
BmiI GGNNCC 2 cut(s) 140, 234
BmsI GCATC 2 cut(s) 511, 723
BpmI CTGGAG 2 cut(s) 125, 219
Bpu10I CCTNAGC 1 cut(s) 543
BsaHI GRCGYC 1 cut(s) 641
BsaJI CCNNGG 3 cut(s) 134, 391, 909
BsaWI WCCGGW 1 cut(s) 729
Bse1I ACTGG 4 cut(s) 32, 142, 236, 802
Bse3DI GCAATG 1 cut(s) 533
BseDI CCNNGG 3 cut(s) 134, 391, 909
BseGI GGATG 2 cut(s) 526, 738
BseMI GCAATG 1 cut(s) 533
BseMII CTCAG 1 cut(s) 534
BseNI ACTGG 4 cut(s) 32, 142, 236, 802
BseRI GAGGAG 1 cut(s) 528
BseYI CCCAGC 1 cut(s) 467
Bsh1236I CGCG 1 cut(s) 97
BshFI GGCC 1 cut(s) 801
BsiSI CCGG 1 cut(s) 730
BsmAI GTCTC 1 cut(s) 648
BsmBI CGTCTC 1 cut(s) 648
BsnI GGCC 1 cut(s) 801
Bsp143I GATC 3 cut(s) 263, 395, 826
BspACI CCGC 2 cut(s) 95, 524
BspANI GGCC 1 cut(s) 801
BspCNI CTCAG 1 cut(s) 535
BspFNI CGCG 1 cut(s) 97
BspLI GGNNCC 2 cut(s) 140, 234
BspMI ACCTGC 1 cut(s) 581
BspPI GGATC 3 cut(s) 271, 403, 821
BsrDI GCAATG 1 cut(s) 533
BsrI ACTGG 4 cut(s) 32, 142, 236, 802
BssECI CCNNGG 3 cut(s) 134, 391, 909
BssMI GATC 3 cut(s) 263, 395, 826
BssNAI GTATAC 1 cut(s) 630
BssNI GRCGYC 1 cut(s) 641
BssT1I CCWWGG 3 cut(s) 134, 391, 909
Bst1107I GTATAC 1 cut(s) 630
Bst4CI ACNGT 4 cut(s) 257, 489, 530, 834
BstACI GRCGYC 1 cut(s) 641
BstDEI CTNAG 3 cut(s) 543, 656, 737
BstF5I GGATG 2 cut(s) 526, 738
BstFNI CGCG 1 cut(s) 97
BstKTI GATC 3 cut(s) 266, 398, 829
BstMAI GTCTC 1 cut(s) 648
BstMBI GATC 3 cut(s) 263, 395, 826
BstMWI GCNNNNNNNGC 7 cut(s) 374, 530, 544, 578, 587, 881, 938
BstNSI RCATGY 1 cut(s) 727
BstUI CGCG 1 cut(s) 97
BstX2I RGATCY 2 cut(s) 395, 826
BstXI CCANNNNNNTGG 2 cut(s) 124, 809
BstYI RGATCY 2 cut(s) 395, 826
BstZ17I GTATAC 1 cut(s) 630
BsuRI GGCC 1 cut(s) 801
BtsCI GGATG 2 cut(s) 526, 738
BtsIMutI CAGTG 1 cut(s) 243
BveI ACCTGC 1 cut(s) 581
Cfr13I GGNCC 2 cut(s) 449, 526
CpoI CGGWCCG 1 cut(s) 526
CseI GACGC 1 cut(s) 630
Csp6I GTAC 9 cut(s) 25, 41, 65, 114, 207, 490, 682, 727, 764
CspI CGGWCCG 1 cut(s) 526
CviAII CATG 4 cut(s) 297, 724, 809, 946
CviQI GTAC 9 cut(s) 25, 41, 65, 114, 207, 490, 682, 727, 764
DdeI CTNAG 3 cut(s) 543, 656, 737
DpnI GATC 3 cut(s) 265, 397, 828
DpnII GATC 3 cut(s) 263, 395, 826
DraI TTTAAA 2 cut(s) 183, 384
EaeI YGGCCR 1 cut(s) 799
Eco130I CCWWGG 3 cut(s) 134, 391, 909
Eco47I GGWCC 2 cut(s) 449, 526
Eco57I CTGAAG 2 cut(s) 21, 668
EcoRI GAATTC 2 cut(s) 51, 788
EcoT14I CCWWGG 3 cut(s) 134, 391, 909
ErhI CCWWGG 3 cut(s) 134, 391, 909
Esp3I CGTCTC 1 cut(s) 648
FaeI CATG 4 cut(s) 300, 727, 812, 949
FatI CATG 4 cut(s) 296, 723, 808, 945
FauNDI CATATG 2 cut(s) 748, 880
FblI GTMKAC 3 cut(s) 603, 629, 742
FokI GGATG 2 cut(s) 533, 745
FspBI CTAG 4 cut(s) 68, 422, 476, 507
GsaI CCCAGC 1 cut(s) 471
GsuI CTGGAG 2 cut(s) 125, 219
HaeIII GGCC 1 cut(s) 801
HapII CCGG 1 cut(s) 730
HgaI GACGC 1 cut(s) 630
Hin1I GRCGYC 1 cut(s) 641
Hin1II CATG 4 cut(s) 300, 727, 812, 949
HincII GTYRAC 1 cut(s) 743
HindII GTYRAC 1 cut(s) 743
HinfI GANTC 3 cut(s) 429, 704, 866
HpaII CCGG 1 cut(s) 730
Hpy166II GTNNAC 5 cut(s) 209, 604, 630, 743, 764
Hpy188I TCNGA 3 cut(s) 400, 648, 787
Hpy188III TCNNGA 3 cut(s) 455, 542, 824
Hpy8I GTNNAC 5 cut(s) 209, 604, 630, 743, 764
Hpy99I CGWCG 1 cut(s) 60
HpyAV CCTTC 1 cut(s) 170
HpyCH4III ACNGT 4 cut(s) 257, 489, 530, 834
HpyCH4V TGCA 3 cut(s) 419, 533, 920
HpyF10VI GCNNNNNNNGC 7 cut(s) 374, 530, 544, 578, 587, 881, 938
HpyF3I CTNAG 3 cut(s) 543, 656, 737
Hsp92I GRCGYC 1 cut(s) 641
Hsp92II CATG 4 cut(s) 300, 727, 812, 949
Kzo9I GATC 3 cut(s) 263, 395, 826
LmnI GCTCC 3 cut(s) 144, 238, 464
LweI GCATC 2 cut(s) 511, 723
MaeI CTAG 4 cut(s) 68, 422, 476, 507
MaeIII GTNAC 3 cut(s) 219, 616, 812
MalI GATC 3 cut(s) 265, 397, 828
MboI GATC 3 cut(s) 263, 395, 826
MboII GAAGA 2 cut(s) 27, 945
MfeI CAATTG 1 cut(s) 369
MflI RGATCY 2 cut(s) 395, 826
MlsI TGGCCA 1 cut(s) 801
MluCI AATT 8 cut(s) 51, 76, 121, 224, 279, 369, 788, 914
MluNI TGGCCA 1 cut(s) 801
MlyI GAGTC 1 cut(s) 438
MnlI CCTC 6 cut(s) 347, 355, 462, 506, 511, 665
Mox20I TGGCCA 1 cut(s) 801
MscI TGGCCA 1 cut(s) 801
MseI TTAA 3 cut(s) 182, 383, 773
MslI CAYNNNNRTG 1 cut(s) 807
Msp20I TGGCCA 1 cut(s) 801
MspI CCGG 1 cut(s) 730
MunI CAATTG 1 cut(s) 369
MvnI CGCG 1 cut(s) 97
MwoI GCNNNNNNNGC 7 cut(s) 374, 530, 544, 578, 587, 881, 938
NdeI CATATG 2 cut(s) 748, 880
NdeII GATC 3 cut(s) 263, 395, 826
NlaIII CATG 4 cut(s) 300, 727, 812, 949
NlaIV GGNNCC 2 cut(s) 140, 234
NmuCI GTSAC 1 cut(s) 812
NspI RCATGY 1 cut(s) 727
PaqCI CACCTGC 1 cut(s) 581
PciI ACATGT 1 cut(s) 723
PfeI GAWTC 2 cut(s) 704, 866
PleI GAGTC 1 cut(s) 437
PpsI GAGTC 1 cut(s) 437
PscI ACATGT 1 cut(s) 723
PspFI CCCAGC 1 cut(s) 467
PspN4I GGNNCC 2 cut(s) 140, 234
PspPI GGNCC 2 cut(s) 449, 526
PsuI RGATCY 2 cut(s) 395, 826
RsaI GTAC 9 cut(s) 26, 42, 66, 115, 208, 491, 683, 728, 765
RsaNI GTAC 9 cut(s) 25, 41, 65, 114, 207, 490, 682, 727, 764
RseI CAYNNNNRTG 1 cut(s) 807
Rsr2I CGGWCCG 1 cut(s) 526
RsrII CGGWCCG 1 cut(s) 526
SalI GTCGAC 1 cut(s) 741
SaqAI TTAA 3 cut(s) 182, 383, 773
Sau3AI GATC 3 cut(s) 263, 395, 826
Sau96I GGNCC 2 cut(s) 449, 526
ScaI AGTACT 1 cut(s) 26
SchI GAGTC 1 cut(s) 438
SetI ASST 9 cut(s) 358, 366, 393, 427, 469, 556, 595, 907, 934
SfaNI GCATC 2 cut(s) 511, 723
SinI GGWCC 2 cut(s) 449, 526
SmiMI CAYNNNNRTG 1 cut(s) 807
Sse9I AATT 8 cut(s) 51, 76, 121, 224, 279, 369, 788, 914
SsiI CCGC 2 cut(s) 95, 524
SspI AATATT 1 cut(s) 187
SspMI CTAG 4 cut(s) 68, 422, 476, 507
StyI CCWWGG 3 cut(s) 134, 391, 909
TaaI ACNGT 4 cut(s) 257, 489, 530, 834
TaqI TCGA 2 cut(s) 55, 742
TasI AATT 8 cut(s) 51, 76, 121, 224, 279, 369, 788, 914
TatI WGTACW 4 cut(s) 24, 206, 681, 763
TfiI GAWTC 2 cut(s) 704, 866
Tru1I TTAA 3 cut(s) 182, 383, 773
Tru9I TTAA 3 cut(s) 182, 383, 773
TscAI CASTG 1 cut(s) 243
TseFI GTSAC 1 cut(s) 812
Tsp45I GTSAC 1 cut(s) 812
TspDTI ATGAA 2 cut(s) 285, 510
TspRI CASTG 1 cut(s) 243
VpaK11BI GGWCC 2 cut(s) 449, 526
XapI RAATTY 3 cut(s) 51, 788, 914
XceI RCATGY 1 cut(s) 727
XcmI CCANNNNNNNNNTGG 1 cut(s) 294
XmiI GTMKAC 3 cut(s) 603, 629, 742
XspI CTAG 4 cut(s) 68, 422, 476, 507
ZrmI AGTACT 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.