Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0004706

Physical Location & Seq

Genomic Coordinates Reverse (-)

60647920 .. 60650338

Gene Structure 2419 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G036840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1098 bp

ATGGCATCTGAACCTGTCAATGTGAATGAATTCCAAGAATTGGCTAGGCAAGTACTTCCAAAAATGTACTATGATTACTATAGTGGGGGAGCTGAGGACCAATTCACGCTAAAAGAGAATGTTGAAGCATTTCGCAAAATCACGTTAAGGCCTAGAGTTCTTGTAGATGTTAGCAGAATCAATATGTCAACCACTATATTGGGTTACAAAGTCTCAGCACCTATAATGATTGCTCCTAGTGCTATGCATCAGTTAGCACACCCTGAAGGAGAGGTCGCTACTGCTAGAGCAGCAGCTGCATCCAATACCATAATGATTTTGTCCTACAATTCTACCTGCACGGTGGAGGAGGTTGCTTCGAGCTGCAATGCTATTCGGTTCTTTCAATTATATATTCACAAGAGACGAGATATATCGGCTCAGATGGTTCAAAGAGCTGAAAAAAATGGATACAAGGCTATTGTCCTGACTGTTGATGTTCCCAGGCTTGGTAGGAGAGAGGCAGACATAAAGAACAAAATGGTTGCTCCTCAGTTGAAGAACTTTGAAGGTCTAATATCAACTGCACTAGACTCTGATGAAGGATCCAACTTGGAAGCTGTTGCCAGAGCGACCTATGATGATTCTTTGTCCTGGGAGGACGTAATAGGATGGTTAAAATCTATTACAAACTTGCCAATTCTAGTCAAGGGTATACTCACTCATGAAGATGCAAGAAAGGCTGTGGAAGTTGGTGTTGCCGGGATTGTGGTCTCTAATCATGGAGCGCGACAACTAAACTACACTCCTGCCACTATTTCTGTCCTGGAAGAGGTCATTCAAGCTGTTGAAGGGAAAGTTCCTGTTCTTTTTGATGGAGGAATACGACGAGGAACTGATGTCTTCAAGGCATTAGCCCTCGGTGCACAAGCCGTCCTTGTTGGAAGGCCTGTTATCTATGGCCTTGCAGCAAATGGAGAAGACGGGGTGAGAAAGGTGATGAAGATGTTGAAGGATGAGTTAGAGCTCACTATGGCTCTCTCTGGCTGTCCTAGTGTTAAGGATATTACCAGGAGCCACGTGAGAACAGAATGTGATAGACTCCGCTCCATGCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0003973 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008891 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009853 GO:0009987 GO:0010109 GO:0010204 GO:0016032 GO:0016491 GO:0016614 GO:0016899 GO:0016999 GO:0017000 GO:0017144 GO:0019048 GO:0019222 GO:0023052 GO:0031323 GO:0031347 GO:0031349 GO:0035821 GO:0042579 GO:0042742 GO:0042743 GO:0043094 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044003 GO:0044237 GO:0044249 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045089 GO:0048518 GO:0048583 GO:0048584 GO:0050665 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051186 GO:0051188 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0055114 GO:0065007 GO:0072593 GO:0080134 GO:0098542 GO:1903409

KEGG Pathways

Metabolic & Signaling

ko00630 ko01100 ko01110 ko01120 ko01130 ko01200 ko04146 map00630 map01100 map01110 map01120 map01130 map01200 map04146

Pfam Domains

Protein Families

FMN_dh

Protein Analysis

365

Amino Acids

40.07

Weight (kDa)

7.02

Isoelectric Point (pI)

43.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FMN_dh	PF01070	15 - 355	2e-134	FMN-dependent dehydrogenase
NMO	PF03060	232 - 344	1.8e-07	Nitronate monooxygenase
IMPDH	PF00478	263 - 309	2.7e-06	IMP dehydrogenase / GMP reductase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004706)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G14130 AT3G14130 AT3G14130 AT3G14150 AT3G14150
fragaria_vesca	FvH4_6g35190 FvH4_6g35192
malus_domestica	MD09G1177800.v1.1 MD17G1148900.v1.1
prunus_persica	Prupe.3G037200_v2.0.a1 Prupe.3G037200_v2.0.a1 Prupe.3G037200_v2.0.a1
pyrus_communis	pycom09g09620 pycom17g14260
rosa_chinensis	RchiOBHm_Chr2g0146451 RchiOBHm_Chr2g0146471 RchiOBHm_Chr2g0146501
rosa_laevigata	RLG00000020202 RLG00000020203
rosa_multiflora	Rmu_co8466583.1_g000001 Rmu_sc0001986.1_g000011 Rmu_sc0024495.1_g000001
rosa_roxburghii	Rroxscaffold_2G00100070 Rroxscaffold_2G00100080
rosa_rugosa	Rorug02G0394600
rosa_samantha	Rh2DG472800 Rh2DG472900
rosa_wichuraiana	Rw2G036840 Rw2G036850 Rw2G036860 Rw2G036870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	344
AccB7I	CCANNNNNTGG	1 cut(s)	40
AccBSI	CCGCTC	1 cut(s)	1086
AccI	GTMKAC	1 cut(s)	694
AccII	CGCG	1 cut(s)	769
AciI	CCGC	1 cut(s)	1084
AclWI	GGATC	2 cut(s)	579, 592
AcsI	RAATTY	1 cut(s)	29
AcuI	CTGAAG	1 cut(s)	285
AcvI	CACGTG	1 cut(s)	1060
AfaI	GTAC	2 cut(s)	54, 68
AfiI	CCNNNNNNNGG	3 cut(s)	40, 488, 811
AgsI	TTSAA	9 cut(s)	125, 386, 431, 538, 548, 821, 830, 886, 991
AjnI	CCWGG	4 cut(s)	482, 632, 804, 1049
AluBI	AGCT	7 cut(s)	92, 296, 363, 437, 599, 824, 1006
AluI	AGCT	7 cut(s)	92, 296, 363, 437, 599, 824, 1006
Alw21I	GWGCWC	2 cut(s)	907, 1008
Alw26I	GTCTC	3 cut(s)	217, 397, 757
Alw44I	GTGCAC	1 cut(s)	903
AlwI	GGATC	2 cut(s)	579, 592
AlwNI	CAGNNNCTG	1 cut(s)	296
AoxI	GGCC	3 cut(s)	149, 926, 940
ApaLI	GTGCAC	1 cut(s)	903
ApeKI	GCWGC	5 cut(s)	290, 293, 296, 363, 947
ApoI	RAATTY	1 cut(s)	29
Asp700I	GAANNNNTTC	2 cut(s)	29, 129
AspLEI	GCGC	1 cut(s)	769
AspS9I	GGNCC	1 cut(s)	97
AsuC2I	CCSGG	1 cut(s)	742
AsuHPI	GGTGA	2 cut(s)	979, 988
AvaII	GGWCC	1 cut(s)	97
BaeGI	GKGCMC	1 cut(s)	907
BamHI	GGATCC	1 cut(s)	584
BanII	GRGCYC	1 cut(s)	1008
BbrPI	CACGTG	1 cut(s)	1060
BbsI	GAAGAC	2 cut(s)	874, 966
Bbv12I	GWGCWC	2 cut(s)	907, 1008
BbvCI	CCTCAGC	1 cut(s)	93
BbvI	GCAGC	5 cut(s)	283, 302, 305, 350, 959
BccI	CCATC	3 cut(s)	418, 645, 848
BceAI	ACGGC	1 cut(s)	896
BciT130I	CCWGG	4 cut(s)	484, 634, 806, 1051
BciVI	GTATCC	1 cut(s)	443
BcnI	CCSGG	1 cut(s)	742
BcoDI	GTCTC	3 cut(s)	217, 397, 757
BfaI	CTAG	7 cut(s)	45, 153, 237, 285, 569, 683, 1032
BfmI	CTRYAG	1 cut(s)	79
BfuAI	ACCTGC	1 cut(s)	344
BfuI	GTATCC	1 cut(s)	443
BisI	GCNGC	5 cut(s)	291, 294, 297, 364, 948
BlsI	GCNGC	5 cut(s)	292, 295, 298, 365, 949
BmcAI	AGTACT	1 cut(s)	54
Bme1390I	CCNGG	5 cut(s)	484, 634, 742, 806, 1051
Bme18I	GGWCC	1 cut(s)	97
BmgT120I	GGNCC	1 cut(s)	97
BmiI	GGNNCC	2 cut(s)	586, 1055
BmrFI	CCNGG	5 cut(s)	484, 634, 742, 806, 1051
BmsI	GCATC	4 cut(s)	14, 256, 308, 700
BpiI	GAAGAC	2 cut(s)	874, 966
Bpu10I	CCTNAGC	1 cut(s)	93
BpuMI	CCSGG	1 cut(s)	742
BsaAI	YACGTR	1 cut(s)	1060
BsaI	GGTCTC	1 cut(s)	757
BsaJI	CCNNGG	3 cut(s)	482, 633, 898
Bsc4I	CCNNNNNNNGG	3 cut(s)	40, 488, 811
Bse3DI	GCAATG	1 cut(s)	373
BseBI	CCWGG	4 cut(s)	484, 634, 806, 1051
BseDI	CCNNGG	3 cut(s)	482, 633, 898
BseGI	GGATG	3 cut(s)	299, 656, 1000
BseLI	CCNNNNNNNGG	3 cut(s)	40, 488, 811
BseMI	GCAATG	1 cut(s)	373
BseMII	CTCAG	4 cut(s)	84, 228, 434, 545
BseRI	GAGGAG	2 cut(s)	362, 519
BseSI	GKGCMC	1 cut(s)	907
BseXI	GCAGC	5 cut(s)	283, 302, 305, 350, 959
BsgI	GTGCAG	2 cut(s)	322, 549
Bsh1236I	CGCG	1 cut(s)	769
BshFI	GGCC	3 cut(s)	151, 928, 942
BsiHKAI	GWGCWC	2 cut(s)	907, 1008
BsiSI	CCGG	1 cut(s)	741
BslI	CCNNNNNNNGG	3 cut(s)	40, 488, 811
BsmAI	GTCTC	3 cut(s)	217, 397, 757
BsmBI	CGTCTC	1 cut(s)	397
BsnI	GGCC	3 cut(s)	151, 928, 942
Bso31I	GGTCTC	1 cut(s)	757
Bsp1286I	GDGCHC	2 cut(s)	907, 1008
Bsp143I	GATC	1 cut(s)	584
BspACI	CCGC	1 cut(s)	1084
BspANI	GGCC	3 cut(s)	151, 928, 942
BspCNI	CTCAG	4 cut(s)	85, 227, 433, 544
BspFNI	CGCG	1 cut(s)	769
BspHI	TCATGA	1 cut(s)	703
BspLI	GGNNCC	2 cut(s)	586, 1055
BspMI	ACCTGC	1 cut(s)	344
BspPI	GGATC	2 cut(s)	579, 592
BspTNI	GGTCTC	1 cut(s)	757
BsrBI	CCGCTC	1 cut(s)	1086
BsrDI	GCAATG	1 cut(s)	373
BssECI	CCNNGG	3 cut(s)	482, 633, 898
BssMI	GATC	1 cut(s)	584
BssNAI	GTATAC	1 cut(s)	695
Bst1107I	GTATAC	1 cut(s)	695
Bst2UI	CCWGG	4 cut(s)	484, 634, 806, 1051
Bst4CI	ACNGT	2 cut(s)	343, 472
Bst6I	CTCTTC	1 cut(s)	804
BstAPI	GCANNNNNTGC	1 cut(s)	296
BstBAI	YACGTR	1 cut(s)	1060
BstDEI	CTNAG	4 cut(s)	93, 214, 420, 531
BstENI	CCTNNNNNAGG	1 cut(s)	809
BstF5I	GGATG	3 cut(s)	299, 656, 1000
BstFNI	CGCG	1 cut(s)	769
BstHHI	GCGC	1 cut(s)	769
BstKTI	GATC	1 cut(s)	587
BstMAI	GTCTC	3 cut(s)	217, 397, 757
BstMBI	GATC	1 cut(s)	584
BstMWI	GCNNNNNNNGC	5 cut(s)	239, 290, 296, 719, 902
BstNI	CCWGG	4 cut(s)	484, 634, 806, 1051
BstSCI	CCNGG	5 cut(s)	482, 632, 740, 804, 1049
BstSFI	CTRYAG	1 cut(s)	79
BstSLI	GKGCMC	1 cut(s)	907
BstUI	CGCG	1 cut(s)	769
BstV1I	GCAGC	5 cut(s)	283, 302, 305, 350, 959
BstV2I	GAAGAC	2 cut(s)	874, 966
BstX2I	RGATCY	1 cut(s)	584
BstXI	CCANNNNNNTGG	1 cut(s)	199
BstYI	RGATCY	1 cut(s)	584
BstZ17I	GTATAC	1 cut(s)	695
BsuI	GTATCC	1 cut(s)	443
BsuRI	GGCC	3 cut(s)	151, 928, 942
BtsCI	GGATG	3 cut(s)	299, 656, 1000
BveI	ACCTGC	1 cut(s)	344
CaiI	CAGNNNCTG	1 cut(s)	296
CciI	TCATGA	1 cut(s)	703
CfoI	GCGC	1 cut(s)	769
Cfr13I	GGNCC	1 cut(s)	97
Csp6I	GTAC	2 cut(s)	53, 67
CviAII	CATG	3 cut(s)	704, 761, 1090
CviQI	GTAC	2 cut(s)	53, 67
DdeI	CTNAG	4 cut(s)	93, 214, 420, 531
DpnI	GATC	1 cut(s)	586
DpnII	GATC	1 cut(s)	584
Eam1104I	CTCTTC	1 cut(s)	804
EarI	CTCTTC	1 cut(s)	804
Ecl136II	GAGCTC	1 cut(s)	1006
Eco147I	AGGCCT	2 cut(s)	151, 928
Eco24I	GRGCYC	1 cut(s)	1008
Eco31I	GGTCTC	1 cut(s)	757
Eco47I	GGWCC	1 cut(s)	97
Eco53kI	GAGCTC	1 cut(s)	1006
Eco57I	CTGAAG	1 cut(s)	285
Eco72I	CACGTG	1 cut(s)	1060
EcoICRI	GAGCTC	1 cut(s)	1006
EcoNI	CCTNNNNNAGG	1 cut(s)	809
EcoRI	GAATTC	1 cut(s)	29
EcoRII	CCWGG	4 cut(s)	482, 632, 804, 1049
EcoT22I	ATGCAT	1 cut(s)	249
EcoT38I	GRGCYC	1 cut(s)	1008
Esp3I	CGTCTC	1 cut(s)	397
FaeI	CATG	3 cut(s)	707, 764, 1093
FalI	AAGNNNNNCTT	2 cut(s)	900, 932
FatI	CATG	3 cut(s)	703, 760, 1089
FblI	GTMKAC	1 cut(s)	694
Fnu4HI	GCNGC	5 cut(s)	291, 294, 297, 364, 948
FokI	GGATG	3 cut(s)	286, 663, 1007
FriOI	GRGCYC	1 cut(s)	1008
Fsp4HI	GCNGC	5 cut(s)	291, 294, 297, 364, 948
FspBI	CTAG	7 cut(s)	45, 153, 237, 285, 569, 683, 1032
GlaI	GCGC	1 cut(s)	768
GluI	GCNGC	5 cut(s)	291, 294, 297, 364, 948
HaeIII	GGCC	3 cut(s)	151, 928, 942
HapII	CCGG	1 cut(s)	741
HhaI	GCGC	1 cut(s)	769
Hin1II	CATG	3 cut(s)	707, 764, 1093
Hin6I	GCGC	1 cut(s)	767
HinP1I	GCGC	1 cut(s)	767
HincII	GTYRAC	1 cut(s)	189
HindII	GTYRAC	1 cut(s)	189
HinfI	GANTC	4 cut(s)	177, 572, 623, 1080
HpaII	CCGG	1 cut(s)	741
HphI	GGTGA	2 cut(s)	979, 988
Hpy166II	GTNNAC	3 cut(s)	189, 695, 905
Hpy188I	TCNGA	3 cut(s)	10, 423, 577
Hpy188III	TCNNGA	2 cut(s)	466, 704
Hpy8I	GTNNAC	3 cut(s)	189, 695, 905
Hpy99I	CGWCG	1 cut(s)	870
HpyAV	CCTTC	6 cut(s)	260, 542, 575, 824, 918, 985
HpyCH4III	ACNGT	2 cut(s)	343, 472
HpyCH4IV	ACGT	3 cut(s)	143, 642, 1059
HpyCH4V	TGCA	8 cut(s)	247, 299, 339, 366, 566, 713, 905, 947
HpyF10VI	GCNNNNNNNGC	5 cut(s)	239, 290, 296, 719, 902
HpyF3I	CTNAG	4 cut(s)	93, 214, 420, 531
HpySE526I	ACGT	3 cut(s)	143, 642, 1059
Hsp92II	CATG	3 cut(s)	707, 764, 1093
HspAI	GCGC	1 cut(s)	767
Kzo9I	GATC	1 cut(s)	584
LmnI	GCTCC	6 cut(s)	89, 238, 532, 764, 1053, 1091
Lsp1109I	GCAGC	5 cut(s)	283, 302, 305, 350, 959
LweI	GCATC	4 cut(s)	14, 256, 308, 700
MaeI	CTAG	7 cut(s)	45, 153, 237, 285, 569, 683, 1032
MaeII	ACGT	3 cut(s)	143, 642, 1059
MaeIII	GTNAC	1 cut(s)	203
MalI	GATC	1 cut(s)	586
MbiI	CCGCTC	1 cut(s)	1086
MboI	GATC	1 cut(s)	584
MboII	GAAGA	6 cut(s)	550, 719, 821, 874, 971, 994
MflI	RGATCY	1 cut(s)	584
MhlI	GDGCHC	2 cut(s)	907, 1008
MluCI	AATT	6 cut(s)	29, 38, 101, 328, 386, 678
MlyI	GAGTC	2 cut(s)	566, 1074
MmeI	TCCRAC	2 cut(s)	612, 901
Mph1103I	ATGCAT	1 cut(s)	249
MroXI	GAANNNNTTC	2 cut(s)	29, 129
MseI	TTAA	4 cut(s)	146, 656, 1038, 1096
MslI	CAYNNNNRTG	1 cut(s)	708
MspA1I	CMGCKG	1 cut(s)	296
MspI	CCGG	1 cut(s)	741
MspR9I	CCNGG	5 cut(s)	484, 634, 742, 806, 1051
MvaI	CCWGG	4 cut(s)	484, 634, 806, 1051
MvnI	CGCG	1 cut(s)	769
MwoI	GCNNNNNNNGC	5 cut(s)	239, 290, 296, 719, 902
NciI	CCSGG	1 cut(s)	742
NdeII	GATC	1 cut(s)	584
NlaIII	CATG	3 cut(s)	707, 764, 1093
NlaIV	GGNNCC	2 cut(s)	586, 1055
NsiI	ATGCAT	1 cut(s)	249
PagI	TCATGA	1 cut(s)	703
PceI	AGGCCT	2 cut(s)	151, 928
PdmI	GAANNNNTTC	2 cut(s)	29, 129
PfeI	GAWTC	2 cut(s)	177, 623
PflMI	CCANNNNNTGG	1 cut(s)	40
PfoI	TCCNGGA	1 cut(s)	804
PkrI	GCNGC	5 cut(s)	292, 295, 298, 365, 949
PleI	GAGTC	2 cut(s)	566, 1074
PmaCI	CACGTG	1 cut(s)	1060
PmlI	CACGTG	1 cut(s)	1060
PpsI	GAGTC	2 cut(s)	566, 1074
Ppu21I	YACGTR	1 cut(s)	1060
Psp124BI	GAGCTC	1 cut(s)	1008
Psp6I	CCWGG	4 cut(s)	482, 632, 804, 1049
PspCI	CACGTG	1 cut(s)	1060
PspGI	CCWGG	4 cut(s)	482, 632, 804, 1049
PspN4I	GGNNCC	2 cut(s)	586, 1055
PspPI	GGNCC	1 cut(s)	97
PstNI	CAGNNNCTG	1 cut(s)	296
PsuI	RGATCY	1 cut(s)	584
PvuII	CAGCTG	1 cut(s)	296
RsaI	GTAC	2 cut(s)	54, 68
RsaNI	GTAC	2 cut(s)	53, 67
RseI	CAYNNNNRTG	1 cut(s)	708
SacI	GAGCTC	1 cut(s)	1008
SaqAI	TTAA	4 cut(s)	146, 656, 1038, 1096
SatI	GCNGC	5 cut(s)	291, 294, 297, 364, 948
Sau3AI	GATC	1 cut(s)	584
Sau96I	GGNCC	1 cut(s)	97
ScaI	AGTACT	1 cut(s)	54
SchI	GAGTC	2 cut(s)	566, 1074
ScrFI	CCNGG	5 cut(s)	484, 634, 742, 806, 1051
SduI	GDGCHC	2 cut(s)	907, 1008
SfaNI	GCATC	4 cut(s)	14, 256, 308, 700
SfcI	CTRYAG	1 cut(s)	79
SinI	GGWCC	1 cut(s)	97
SmiMI	CAYNNNNRTG	1 cut(s)	708
Sse9I	AATT	6 cut(s)	29, 38, 101, 328, 386, 678
SseBI	AGGCCT	2 cut(s)	151, 928
SsiI	CCGC	1 cut(s)	1084
SspMI	CTAG	7 cut(s)	45, 153, 237, 285, 569, 683, 1032
SstI	GAGCTC	1 cut(s)	1008
StuI	AGGCCT	2 cut(s)	151, 928
StyD4I	CCNGG	5 cut(s)	482, 632, 740, 804, 1049
TaaI	ACNGT	2 cut(s)	343, 472
TaiI	ACGT	3 cut(s)	146, 645, 1062
TaqI	TCGA	1 cut(s)	359
TasI	AATT	6 cut(s)	29, 38, 101, 328, 386, 678
TatI	WGTACW	2 cut(s)	52, 66
TfiI	GAWTC	2 cut(s)	177, 623
Tru1I	TTAA	4 cut(s)	146, 656, 1038, 1096
Tru9I	TTAA	4 cut(s)	146, 656, 1038, 1096
TseI	GCWGC	5 cut(s)	290, 293, 296, 363, 947
TspDTI	ATGAA	4 cut(s)	42, 594, 720, 995
Van91I	CCANNNNNTGG	1 cut(s)	40
VneI	GTGCAC	1 cut(s)	903
VpaK11BI	GGWCC	1 cut(s)	97
XagI	CCTNNNNNAGG	1 cut(s)	809
XapI	RAATTY	1 cut(s)	29
XmiI	GTMKAC	1 cut(s)	694
XmnI	GAANNNNTTC	2 cut(s)	29, 129
XspI	CTAG	7 cut(s)	45, 153, 237, 285, 569, 683, 1032
ZrmI	AGTACT	1 cut(s)	54
Zsp2I	ATGCAT	1 cut(s)	249

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.