Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0004583

Physical Location & Seq

Genomic Coordinates Forward (+)

53850237 .. 53859798

Gene Structure 9562 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G032070.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3915 bp

ATGTCGCCGGCGTTGCTAGAGTCCCGAGCTCCGGCGCACCACCCTAATCTGAACACCGGTCAGATTTCTCACCCTCCTTTCTCCTTGTTCAACTTTTCTGAATATAAATTTAAGATGAACCCATCGAATGCTAGTGATGTGTCCTCTGGATTTGGGAACTCTAGTCACGATTTTAGCTTCAATTCGTCGTCCCCTTCGCGCCAAGGCTCGGGTCTTGCGAGGCCGAGGCTTTTGAAGGTCAGGAAAGGCCTCATCAATCCGCAGTTATTGAGACCCTCGGCCGAAACCGGGGCTCCTCCTCCGGGATTTAATCCCTTTGGGAATTCCGTTTCTGGGGAATTCAGGAGTGATTCCGATTCGAATTTGAATCCGGGGATAGGAGTGGCTGACCAAATGAGCAATTTGAAAATAATTGGGAGTGGCAGTGAGTTTGGGGATGCCAAAGATGCTGCTGGTTTTCATTTTGATACAGGAACTAGGTTGAGTTCGAGTTCGACTTCAGCTGGGAGGTTGGATGGAGGTGGTTTTGTATTTGGAAGTGAGTTTTTCAAGAGTTCTGGTTTTGATTACGGCATTGCGTCGAAGCTTCCTGAGGATATGAGCAAGTTGAATATTGAGGGCCCTGGGAATGGTGGAGCTGTTGAGAAAGGTGAGGATCGGAGGTTTGATGGAGGTGCAAATACCTTAACCGGGTTTGGGCTGGGGAGTAATGACAATGTGGGTGGTTCATTGGGTAGAAACGTGGGATCGGAACTCCCCCACGAATTGGAGAAGAAGTTGAACATTAACGAGAATGTGCAGATGGGCGGCAGGACTGACACGCATTATGCCACTGGTGTGAATAAGTTTGTGTTTAATACTAGTAAGAAGGATGACTATTCCTCTGCTGGAAGCTCGGTGAATGTACTTCCAGATAAGATGAAGAATCTGAACATAGGTCTTAGTTTTGATGGAAGAAAAGAGAGTCTTCTGTTGAGAAAGATGGAGACTTTGGATATAGGAACTAAGGCTGGACACTCTACTCAATCAGATACAGGGACATCTTCCCGCCAAACATCTGTGAAGAACATGGAAACTGAGAGGGAGGATGAATTTAATTTTACAAGCAAACAAGATCATTTGAGCACATCTTCCGTAGAGTTTAAAACACCAAACACTAAAGCCAATTTGTTTTCGGGCATAAACAAGAAATTGGAATTCAATGCAAGGCGGGAACCAGCAAGGGCTAGGCCCGCTAGGGATACAAGAATGAAAAAAGCAAGTGGAAAATTAAGACGTCCTACTTCATCCCAGCTGGGGCTTGGGCATGATGTTGTTTCAAAAGGTGGTTCTCCAGTAAACGTTGAGGCTTTTGAATCCTATTCGCCAATGGACATTTCTCCTTATCAAGAAACATTGGCTGATCACCAATATTCGAAAGAAAATTCTGCATCTTCCGAGTCATTTAGTCTCGTCAACGATTATCCGGAAACCGATTCAGTTCCAAAAGTTACAAATAATTCTATTGATGAAGATCTTGCTATGGCAACAGAATGTCTGGATATAAATAAAGTTGATGCAGTGAGCAGACCTCCACAAGAGGAAGCTTTTGAGCACCATTTAGGTGGAAGTGTTAATGCTGATGAACCTGTTGAAGGATCTGTTTCTGGGGCTGAAACCGAAAGCTTTAAGTCTGCAACTGACGAGGTAGACTATATCAGTGACACCGCTAATTCAGCGGAGACTGAAGTTAGTTCAAGCCCAAATATGGAGAGACATGATACTGATGGCAGGATACACTTTGGCTTTCATGCAAGTTCATTGAATAGAAGTGGATTAAACTTCACGTTTGCTGCCTCTACTGCTGCTCAATGTCCATTATCACCATCAAAACGTTTGCACAAAAAGAAAAATATGGTAAAGGCTGGTCAAGATGCCAATAGTTTTGTTCCAAATGCAAAGGTTCCATATGGATCATCCTCTGCAGAGTTTTTGCCATATTCTGGAGCTCCAGTACGTTCAACTCCAGGACACTATCAGGAAATAAACCCTCCAATTTCAGTTATTTCACAATGCAATGAAGATAATTCTGGGGTGCAGAAGGAAAAGGAGATCAAACAAGAGGCTGTCTCTTTGTCTGCTGAAACAGCTGCAGCTCAGGAAGCATGTGAGAAGTGGCGACTAAGGGGTAACCAAGCATATTCTAATGGGGATCTATCTAAAGCCGAGGACTGCTATACCCAAGGGGTAAATGGTGTTTCTGAAAATGAGACATCTACAAGCTGTCTCAGGGCACTGATGCTGTGCTATAGCAACCGTGCAGCAACACGCATGTCTCTTGGAAGAATTCGAGATGCACTTGGAGATTGTATGATGGCTGCTGCAATAGATCCTAACTTCCTCAAAGTGCAAGTTAGAGCTGCCAACTGTTATCTTACCCTTGGGGAGGTTCAAGATGCTTCAAAACGTTTTAGTAGGTGCCTCCAATTGGCAAGTGATGTCTCTGTCGACCAAAAGATTGCAGTAGAAGCCTCTGATGGGTTACAAAAGGCGCAGAAAGTGTCTGAATGTCTGAATCTCTGTGCTGAGCTTATGCAAAGGAAGACATCTACCAATGCAGAGAGTGCTTTGGAACTTATTGCTGAGGCTTTGGCAATTAGCCCTAGCTCAGAAAAATTATTTGAAATGAAAGCAGAGGCTCTTTTCACGATGCGGAGGTATGATGAGGTGATTGAGCTTTGTGAGAAGACCCTTGGTTATGCTGAAAAGAACTCCCCTTTAGTAGATACCGCCATTTCTTTAGATGGATCTGAACTCTCAAAGTACTTGCACTTCAGACTTTGGCGGTGTCGTTTGACTTTTAAATCCTATTTCCATCTAGGAAAACTTGAGGAGGGCCTTGCTTCACTAGAGAAACAAGAGGAAAAGTTTTCTACCACGTATAGGAATTGGAGAAAAACTCTGGAATCATCTATTCCCCTTGTTCACATTGTTCGTGAGCTGTTATCTTATAAGGTTGCAGGAAATGAAGCATTTCAGGCTGGGAGGCATACTGAAGCTGTTGAACGTTATACTACTGCTTTGTCATGTAATGCGGAATCTCGTCCTTTCACAGCTGTTTGTTTTTGCAATCGTGCTGCTGCATACAAAGCGCTGGGTCAGATTACTGACGCTATTGCAGATTGCAGCCTAGCTATAGCTCTTGATGGAAGTTATCTAAAGGCTATTTCTAGGCGAGCTACATTATATGAGATGATCAGAGATTATGGACAAGCAGCTAAAGATCTTCATAGACTAGTATCTCTTCTCACTAAGCAGTTAGAGGAAAATATCAATCAATGTGGAACCTCTGATATATCCAATAGCTGCAAAAGTGATTTGAAACAAGCTCGTCTTCGTCTTTCAGAAATAGAAGAAGAAGGCAGAAAGGACATCCCCTTGGATATGTACATTATTCTTGGAATCAAACCATCTATTTCAGCATCAGAAATTAAGAAGGCCTATCGGAAAGCTGCACTCAGACATCATCCTGACAAGGCTGTACAATTCTTTGCTAGGAGTGAAACTGGTGATGATGGGCTTTGGAAGGAGATAGCAGAAGAAGCGCACAAGGATGCTGACAGGCTTTTCAAAATGATTGGGGAGGCATATGCAGTACTTTCAGACTCTACCAAGCGTGCACGGTATGATGCTGAAGAGGAGACTAGGAATGCGCAGAAGAAACGCACTGGAAGCAGTGCAGCTAGAATGCCTGCAGATGCTCAAAATTACCCATTTGAAAGAAGCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCCAATTTGGTTCTGGTTTCGAATTCGATCAACTAGGTCAAACGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001671 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006457 GO:0006458 GO:0008047 GO:0008150 GO:0009987 GO:0030234 GO:0031072 GO:0031974 GO:0031981 GO:0032781 GO:0043085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043462 GO:0044093 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0048583 GO:0050789 GO:0050790 GO:0050794 GO:0051084 GO:0051085 GO:0051336 GO:0051345 GO:0060589 GO:0060590 GO:0061077 GO:0065007 GO:0065009 GO:0070013 GO:0080134 GO:0080135 GO:0098772 GO:1900034

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DnaJ TPR_8

Protein Analysis

1304

Amino Acids

138.72

Weight (kDa)

5.92

Isoelectric Point (pI)

45.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DnaJ	PF00226	1141 - 1223	1.5e-15	DnaJ domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004583)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12430
fragaria_vesca	FvH4_3g28360 FvH4_3g28360 FvH4_3g28360 FvH4_3g28360
malus_domestica	MD03G1171100.v1.1 MD03G1171300.v1.1 MD11G1188500.v1.1 MD11G1188700.v1.1
prunus_persica	Prupe.4G255700_v2.0.a1 Prupe.4G255700_v2.0.a1 Prupe.4G255700_v2.0.a1 Prupe.4G255700_v2.0.a1
pyrus_communis	pycom03g12820 pycom03g12830 pycom03g12840 pycom03g12860 pycom11g16530 pycom15g27190
rosa_chinensis	RchiOBHm_Chr5g0053371
rosa_laevigata	RLG00000034766
rosa_multiflora	Rmu_sc0001966.1_g000003
rosa_roxburghii	Rroxscaffold_1G00029100
rosa_rugosa	Rorug05G0267200 Rorug05G0267300
rosa_samantha	Rh5AG339800 Rh5BG350200 Rh5CG376600 Rh5DG364200
rosa_wichuraiana	Rw5G032070 Rw5G032080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2991
AatII	GACGTC	1 cut(s)	1279
Acc16I	TGCGCA	1 cut(s)	3693
AccB1I	GGYRCC	1 cut(s)	2457
AccB7I	CCANNNNNTGG	2 cut(s)	766, 1982
AccI	GTMKAC	2 cut(s)	1689, 2487
AccII	CGCG	1 cut(s)	199
AccIII	TCCGGA	1 cut(s)	1465
AclI	AACGTT	4 cut(s)	1341, 1873, 2446, 3046
AclWI	GGATC	7 cut(s)	663, 754, 1645, 1960, 2199, 2363, 2794
AcoI	YGGCCR	1 cut(s)	279
AcsI	RAATTY	9 cut(s)	107, 322, 338, 361, 1091, 1196, 1423, 2325, 3890
AcuI	CTGAAG	5 cut(s)	483, 1746, 2797, 3054, 3693
AcyI	GRCGYC	1 cut(s)	1276
AfaI	GTAC	6 cut(s)	906, 1995, 2804, 3428, 3522, 3636
AfeI	AGCGCT	1 cut(s)	3132
AgeI	ACCGGT	1 cut(s)	56
AhlI	ACTAGT	2 cut(s)	860, 3274
AjnI	CCWGG	2 cut(s)	622, 2005
AjuI	GAANNNNNNNTTGG	2 cut(s)	1360, 1392
AleI	CACNNNNGTG	1 cut(s)	836
Alw21I	GWGCWC	5 cut(s)	31, 1127, 1596, 1990, 3661
Alw44I	GTGCAC	1 cut(s)	3657
AlwI	GGATC	7 cut(s)	663, 754, 1645, 1960, 2199, 2363, 2794
Ama87I	CYCGRG	2 cut(s)	24, 208
Aor13HI	TCCGGA	1 cut(s)	1465
Aor51HI	AGCGCT	1 cut(s)	3132
AoxI	GGCC	7 cut(s)	221, 247, 279, 619, 1229, 2875, 3477
ApaI	GGGCCC	1 cut(s)	623
ApaLI	GTGCAC	1 cut(s)	3657
ApoI	RAATTY	9 cut(s)	107, 322, 338, 361, 1091, 1196, 1423, 2325, 3890
AsiGI	ACCGGT	1 cut(s)	56
Asp700I	GAANNNNTTC	1 cut(s)	3012
AspLEI	GCGC	6 cut(s)	37, 201, 2533, 3133, 3586, 3694
AspS9I	GGNCC	4 cut(s)	619, 620, 1230, 2875
AsuC2I	CCSGG	4 cut(s)	289, 303, 372, 691
AsuHPI	GGTGA	7 cut(s)	62, 662, 910, 1397, 1854, 2719, 3560
AsuII	TTCGAA	3 cut(s)	359, 1415, 3888
AvaI	CYCGRG	2 cut(s)	24, 208
AxyI	CCTNAGG	1 cut(s)	591
BaeGI	GKGCMC	3 cut(s)	623, 2275, 3661
BanI	GGYRCC	1 cut(s)	2457
BanII	GRGCYC	4 cut(s)	31, 295, 623, 1990
BarI	GAAGNNNNNNTAC	2 cut(s)	2795, 2827
BbsI	GAAGAC	4 cut(s)	959, 2588, 2732, 3365
Bbv12I	GWGCWC	5 cut(s)	31, 1127, 1596, 1990, 3661
BbvCI	CCTCAGC	1 cut(s)	2622
BceAI	ACGGC	1 cut(s)	586
BciT130I	CCWGG	2 cut(s)	624, 2007
BciVI	GTATCC	2 cut(s)	1234, 1767
BclI	TGATCA	2 cut(s)	1402, 3234
BcnI	CCSGG	4 cut(s)	289, 303, 372, 691
BcuI	ACTAGT	2 cut(s)	860, 3274
BfmI	CTRYAG	5 cut(s)	1962, 2130, 2287, 3174, 3732
BfoI	RGCGCY	1 cut(s)	3134
BfuI	GTATCC	2 cut(s)	1234, 1767
BglII	AGATCT	2 cut(s)	1513, 3262
BlpI	GCTNAGC	1 cut(s)	2565
BmcAI	AGTACT	2 cut(s)	2804, 3636
Bme1390I	CCNGG	6 cut(s)	289, 303, 372, 624, 691, 2007
BmeT110I	CYCGRG	2 cut(s)	24, 208
BmgT120I	GGNCC	4 cut(s)	619, 620, 1230, 2875
BmiI	GGNNCC	6 cut(s)	294, 621, 1215, 1944, 2459, 3325
BmrFI	CCNGG	6 cut(s)	289, 303, 372, 624, 691, 2007
BpiI	GAAGAC	4 cut(s)	959, 2588, 2732, 3365
BplI	GAGNNNNNCTC	6 cut(s)	1555, 1587, 2093, 2125, 2923, 2955
BpmI	CTGGAG	4 cut(s)	1317, 1974, 1989, 2004
Bpu10I	CCTNAGC	2 cut(s)	2136, 2622
Bpu1102I	GCTNAGC	1 cut(s)	2565
Bpu14I	TTCGAA	3 cut(s)	359, 1415, 3888
BpuEI	CTTGAG	1 cut(s)	2888
BpuMI	CCSGG	4 cut(s)	289, 303, 372, 691
BsaAI	YACGTR	1 cut(s)	2919
BsaBI	GATNNNNATC	1 cut(s)	1512
BsaHI	GRCGYC	1 cut(s)	1276
BsaI	GGTCTC	1 cut(s)	265
BsaWI	WCCGGW	2 cut(s)	56, 1465
BsaXI	ACNNNNNCTCC	8 cut(s)	277, 307, 409, 439, 761, 791, 1977, 2007
Bse118I	RCCGGY	2 cut(s)	7, 56
Bse1I	ACTGG	5 cut(s)	838, 1334, 1991, 3550, 3712
Bse21I	CCTNAGG	1 cut(s)	591
Bse3DI	GCAATG	2 cut(s)	573, 2062
Bse8I	GATNNNNATC	1 cut(s)	1512
BseAI	TCCGGA	1 cut(s)	1465
BseBI	CCWGG	2 cut(s)	624, 2007
BseGI	GGATG	9 cut(s)	442, 520, 877, 1093, 1286, 1955, 3411, 3505, 3598
BseJI	GATNNNNATC	1 cut(s)	1512
BseMI	GCAATG	2 cut(s)	573, 2062
BseMII	CTCAG	8 cut(s)	582, 1068, 2150, 2281, 2556, 2613, 2661, 3511
BseNI	ACTGG	5 cut(s)	838, 1334, 1991, 3550, 3712
BseRI	GAGGAG	4 cut(s)	285, 288, 2885, 3692
BseSI	GKGCMC	3 cut(s)	623, 2275, 3661
BseX3I	CGGCCG	1 cut(s)	279
BseYI	CCCAGC	6 cut(s)	503, 700, 1290, 1294, 3020, 3133
BsgI	GTGCAG	5 cut(s)	818, 2096, 2319, 3477, 3738
Bsh1236I	CGCG	1 cut(s)	199
Bsh1285I	CGRYCG	1 cut(s)	282
BshFI	GGCC	7 cut(s)	223, 249, 281, 621, 1231, 2877, 3479
BshNI	GGYRCC	1 cut(s)	2457
BshTI	ACCGGT	1 cut(s)	56
BsiEI	CGRYCG	1 cut(s)	282
BsiHKAI	GWGCWC	5 cut(s)	31, 1127, 1596, 1990, 3661
BsiHKCI	CYCGRG	2 cut(s)	24, 208
BsiSI	CCGG	8 cut(s)	8, 32, 57, 288, 302, 371, 690, 1466
BslFI	GGGAC	3 cut(s)	7, 175, 1051
BsmFI	GGGAC	3 cut(s)	7, 175, 1051
BsmI	GAATGC	3 cut(s)	133, 3694, 3732
BsnI	GGCC	7 cut(s)	223, 249, 281, 621, 1231, 2877, 3479
Bso31I	GGTCTC	1 cut(s)	265
BsoBI	CYCGRG	2 cut(s)	24, 208
Bsp119I	TTCGAA	3 cut(s)	359, 1415, 3888
Bsp120I	GGGCCC	1 cut(s)	619
Bsp1286I	GDGCHC	8 cut(s)	31, 295, 623, 1127, 1596, 1990, 2275, 3661
Bsp13I	TCCGGA	1 cut(s)	1465
Bsp1407I	TGTACA	2 cut(s)	3426, 3520
Bsp1720I	GCTNAGC	1 cut(s)	2565
BspANI	GGCC	7 cut(s)	223, 249, 281, 621, 1231, 2877, 3479
BspCNI	CTCAG	8 cut(s)	583, 1069, 2149, 2280, 2557, 2614, 2660, 3510
BspEI	TCCGGA	1 cut(s)	1465
BspFNI	CGCG	1 cut(s)	199
BspLI	GGNNCC	6 cut(s)	294, 621, 1215, 1944, 2459, 3325
BspMAI	CTGCAG	3 cut(s)	1966, 2134, 3736
BspPI	GGATC	7 cut(s)	663, 754, 1645, 1960, 2199, 2363, 2794
BspT104I	TTCGAA	3 cut(s)	359, 1415, 3888
BspT107I	GGYRCC	1 cut(s)	2457
BspTNI	GGTCTC	1 cut(s)	265
BsrDI	GCAATG	2 cut(s)	573, 2062
BsrFI	RCCGGY	2 cut(s)	7, 56
BsrGI	TGTACA	2 cut(s)	3426, 3520
BsrI	ACTGG	5 cut(s)	838, 1334, 1991, 3550, 3712
BssAI	RCCGGY	2 cut(s)	7, 56
BssNI	GRCGYC	1 cut(s)	1276
BssT1I	CCWWGG	5 cut(s)	202, 2221, 2419, 2731, 3417
Bst2UI	CCWGG	2 cut(s)	624, 2007
Bst4CI	ACNGT	3 cut(s)	2297, 2408, 3663
Bst6I	CTCTTC	2 cut(s)	3288, 3669
BstACI	GRCGYC	1 cut(s)	1276
BstAPI	GCANNNNNTGC	1 cut(s)	2603
BstAUI	TGTACA	2 cut(s)	3426, 3520
BstBAI	YACGTR	1 cut(s)	2919
BstBI	TTCGAA	3 cut(s)	359, 1415, 3888
BstC8I	GCNNGC	5 cut(s)	9, 1233, 3216, 3657, 3732
BstEII	GGTNACC	1 cut(s)	2168
BstENI	CCTNNNNNAGG	2 cut(s)	1632, 2423
BstF5I	GGATG	9 cut(s)	442, 520, 877, 1093, 1286, 1955, 3411, 3505, 3598
BstFNI	CGCG	1 cut(s)	199
BstH2I	RGCGCY	1 cut(s)	3134
BstHHI	GCGC	6 cut(s)	37, 201, 2533, 3133, 3586, 3694
BstMCI	CGRYCG	1 cut(s)	282
BstNI	CCWGG	2 cut(s)	624, 2007
BstNSI	RCATGY	2 cut(s)	2148, 2314
BstPI	GGTNACC	1 cut(s)	2168
BstSCI	CCNGG	6 cut(s)	287, 301, 370, 622, 689, 2005
BstSFI	CTRYAG	5 cut(s)	1962, 2130, 2287, 3174, 3732
BstSLI	GKGCMC	3 cut(s)	623, 2275, 3661
BstUI	CGCG	1 cut(s)	199
BstV2I	GAAGAC	4 cut(s)	959, 2588, 2732, 3365
BstX2I	RGATCY	6 cut(s)	1513, 1637, 2191, 2368, 2786, 3262
BstXI	CCANNNNNNTGG	1 cut(s)	1604
BstYI	RGATCY	6 cut(s)	1513, 1637, 2191, 2368, 2786, 3262
BstZI	CGGCCG	1 cut(s)	279
Bsu36I	CCTNAGG	1 cut(s)	591
BsuI	GTATCC	2 cut(s)	1234, 1767
BsuRI	GGCC	7 cut(s)	223, 249, 281, 621, 1231, 2877, 3479
BtsCI	GGATG	9 cut(s)	442, 520, 877, 1093, 1286, 1955, 3411, 3505, 3598
BtsI	GCAGTG	3 cut(s)	430, 1566, 3721
BtsIMutI	CAGTG	7 cut(s)	430, 831, 1566, 1705, 2273, 3705, 3721
Cac8I	GCNNGC	5 cut(s)	9, 1233, 3216, 3657, 3732
CfoI	GCGC	6 cut(s)	37, 201, 2533, 3133, 3586, 3694
Cfr10I	RCCGGY	2 cut(s)	7, 56
Cfr13I	GGNCC	4 cut(s)	619, 620, 1230, 2875
CseI	GACGC	2 cut(s)	567, 3158
Csp6I	GTAC	6 cut(s)	905, 1994, 2803, 3427, 3521, 3635
CspAI	ACCGGT	1 cut(s)	56
CspCI	CAANNNNNGTGG	4 cut(s)	703, 738, 3301, 3336
CviAII	CATG	7 cut(s)	1069, 1307, 1757, 1790, 2145, 2311, 3066
CviQI	GTAC	6 cut(s)	905, 1994, 2803, 3427, 3521, 3635
DraI	TTTAAA	2 cut(s)	1144, 2842
EaeI	YGGCCR	1 cut(s)	279
EagI	CGGCCG	1 cut(s)	279
Eam1104I	CTCTTC	2 cut(s)	3288, 3669
EarI	CTCTTC	2 cut(s)	3288, 3669
Ecl136II	GAGCTC	2 cut(s)	29, 1988
EclXI	CGGCCG	1 cut(s)	279
Eco130I	CCWWGG	5 cut(s)	202, 2221, 2419, 2731, 3417
Eco147I	AGGCCT	2 cut(s)	249, 3479
Eco24I	GRGCYC	4 cut(s)	31, 295, 623, 1990
Eco31I	GGTCTC	1 cut(s)	265
Eco47III	AGCGCT	1 cut(s)	3132
Eco52I	CGGCCG	1 cut(s)	279
Eco53kI	GAGCTC	2 cut(s)	29, 1988
Eco57I	CTGAAG	5 cut(s)	483, 1746, 2797, 3054, 3693
Eco81I	CCTNAGG	1 cut(s)	591
Eco88I	CYCGRG	2 cut(s)	24, 208
Eco91I	GGTNACC	1 cut(s)	2168
EcoICRI	GAGCTC	2 cut(s)	29, 1988
EcoNI	CCTNNNNNAGG	2 cut(s)	1632, 2423
EcoO109I	RGGNCCY	3 cut(s)	619, 620, 2875
EcoO65I	GGTNACC	1 cut(s)	2168
EcoRI	GAATTC	5 cut(s)	322, 338, 1196, 2325, 3890
EcoRII	CCWGG	2 cut(s)	622, 2005
EcoT14I	CCWWGG	5 cut(s)	202, 2221, 2419, 2731, 3417
EcoT38I	GRGCYC	4 cut(s)	31, 295, 623, 1990
ErhI	CCWWGG	5 cut(s)	202, 2221, 2419, 2731, 3417
FaeI	CATG	7 cut(s)	1072, 1310, 1760, 1793, 2148, 2314, 3069
FalI	AAGNNNNNCTT	4 cut(s)	951, 983, 3357, 3389
FaqI	GGGAC	3 cut(s)	7, 175, 1051
FatI	CATG	7 cut(s)	1068, 1306, 1756, 1789, 2144, 2310, 3065
FauI	CCCGC	3 cut(s)	1055, 1203, 1240
FauNDI	CATATG	2 cut(s)	1948, 3628
FbaI	TGATCA	2 cut(s)	1402, 3234
FblI	GTMKAC	2 cut(s)	1689, 2487
FokI	GGATG	9 cut(s)	449, 527, 884, 1100, 1273, 1942, 3398, 3492, 3605
FriOI	GRGCYC	4 cut(s)	31, 295, 623, 1990
FspI	TGCGCA	1 cut(s)	3693
GlaI	GCGC	6 cut(s)	36, 200, 2532, 3132, 3585, 3693
GsaI	CCCAGC	6 cut(s)	507, 704, 1294, 1298, 3024, 3137
GsuI	CTGGAG	4 cut(s)	1317, 1974, 1989, 2004
HaeII	RGCGCY	1 cut(s)	3134
HaeIII	GGCC	7 cut(s)	223, 249, 281, 621, 1231, 2877, 3479
HapII	CCGG	8 cut(s)	8, 32, 57, 288, 302, 371, 690, 1466
HgaI	GACGC	2 cut(s)	567, 3158
HhaI	GCGC	6 cut(s)	37, 201, 2533, 3133, 3586, 3694
Hin1I	GRCGYC	1 cut(s)	1276
Hin1II	CATG	7 cut(s)	1072, 1310, 1760, 1793, 2148, 2314, 3069
Hin6I	GCGC	6 cut(s)	35, 199, 2531, 3131, 3584, 3692
HinP1I	GCGC	6 cut(s)	35, 199, 2531, 3131, 3584, 3692
HincII	GTYRAC	2 cut(s)	1456, 2488
HindII	GTYRAC	2 cut(s)	1456, 2488
HindIII	AAGCTT	3 cut(s)	584, 1584, 1663
HpaII	CCGG	8 cut(s)	8, 32, 57, 288, 302, 371, 690, 1466
HphI	GGTGA	7 cut(s)	62, 662, 910, 1397, 1854, 2719, 3560
Hpy166II	GTNNAC	6 cut(s)	1339, 1456, 1690, 2488, 2965, 3659
Hpy8I	GTNNAC	6 cut(s)	1339, 1456, 1690, 2488, 2965, 3659
Hpy99I	CGWCG	2 cut(s)	190, 583
HpyAV	CCTTC	8 cut(s)	204, 229, 862, 1628, 2074, 3392, 3469, 3559
HpyCH4III	ACNGT	3 cut(s)	2297, 2408, 3663
Hsp92I	GRCGYC	1 cut(s)	1276
Hsp92II	CATG	7 cut(s)	1072, 1310, 1760, 1793, 2148, 2314, 3069
HspAI	GCGC	6 cut(s)	35, 199, 2531, 3131, 3584, 3692
Kpn2I	TCCGGA	1 cut(s)	1465
KroI	GCCGGC	1 cut(s)	7
KroNI	GCCGGC	1 cut(s)	9
Ksp22I	TGATCA	2 cut(s)	1402, 3234
LmnI	GCTCC	5 cut(s)	34, 298, 635, 1985, 1993
MaeIII	GTNAC	5 cut(s)	164, 1489, 1700, 2168, 2520
MfeI	CAATTG	1 cut(s)	2465
MflI	RGATCY	6 cut(s)	1513, 1637, 2191, 2368, 2786, 3262
MhlI	GDGCHC	8 cut(s)	31, 295, 623, 1127, 1596, 1990, 2275, 3661
MlyI	GAGTC	4 cut(s)	29, 973, 1448, 3638
MmeI	TCCRAC	1 cut(s)	492
MreI	CGCCGGCG	1 cut(s)	7
MroI	TCCGGA	1 cut(s)	1465
MroNI	GCCGGC	1 cut(s)	7
MroXI	GAANNNNTTC	1 cut(s)	3012
MslI	CAYNNNNRTG	3 cut(s)	836, 1602, 3591
MspA1I	CMGCKG	5 cut(s)	503, 1294, 1718, 2129, 3095
MspI	CCGG	8 cut(s)	8, 32, 57, 288, 302, 371, 690, 1466
MspR9I	CCNGG	6 cut(s)	289, 303, 372, 624, 691, 2007
MunI	CAATTG	1 cut(s)	2465
Mva1269I	GAATGC	3 cut(s)	133, 3694, 3732
MvaI	CCWGG	2 cut(s)	624, 2007
MvnI	CGCG	1 cut(s)	199
NaeI	GCCGGC	1 cut(s)	9
NciI	CCSGG	4 cut(s)	289, 303, 372, 691
NdeI	CATATG	2 cut(s)	1948, 3628
NgoMIV	GCCGGC	1 cut(s)	7
NlaIII	CATG	7 cut(s)	1072, 1310, 1760, 1793, 2148, 2314, 3069
NlaIV	GGNNCC	6 cut(s)	294, 621, 1215, 1944, 2459, 3325
NmeAIII	GCCGAG	3 cut(s)	249, 257, 2230
NmuCI	GTSAC	2 cut(s)	164, 1700
NsbI	TGCGCA	1 cut(s)	3693
NspI	RCATGY	2 cut(s)	2148, 2314
NspV	TTCGAA	3 cut(s)	359, 1415, 3888
OliI	CACNNNNGTG	1 cut(s)	836
PasI	CCCWGGG	1 cut(s)	623
PceI	AGGCCT	2 cut(s)	249, 3479
PcsI	WCGNNNNNNNCGW	1 cut(s)	215
PctI	GAATGC	3 cut(s)	133, 3694, 3732
PdiI	GCCGGC	1 cut(s)	9
PdmI	GAANNNNTTC	1 cut(s)	3012
PflMI	CCANNNNNTGG	2 cut(s)	766, 1982
PfoI	TCCNGGA	2 cut(s)	301, 2005
PinAI	ACCGGT	1 cut(s)	56
PleI	GAGTC	4 cut(s)	28, 972, 1447, 3638
PpsI	GAGTC	4 cut(s)	28, 972, 1447, 3638
Ppu21I	YACGTR	1 cut(s)	2919
PsiI	TTATAA	1 cut(s)	2991
Psp124BI	GAGCTC	2 cut(s)	31, 1990
Psp1406I	AACGTT	4 cut(s)	1341, 1873, 2446, 3046
Psp6I	CCWGG	2 cut(s)	622, 2005
PspEI	GGTNACC	1 cut(s)	2168
PspFI	CCCAGC	6 cut(s)	503, 700, 1290, 1294, 3020, 3133
PspGI	CCWGG	2 cut(s)	622, 2005
PspN4I	GGNNCC	6 cut(s)	294, 621, 1215, 1944, 2459, 3325
PspOMI	GGGCCC	1 cut(s)	619
PspPI	GGNCC	4 cut(s)	619, 620, 1230, 2875
PstI	CTGCAG	3 cut(s)	1966, 2134, 3736
PsuI	RGATCY	6 cut(s)	1513, 1637, 2191, 2368, 2786, 3262
PvuII	CAGCTG	4 cut(s)	503, 1294, 2129, 3095
RsaI	GTAC	6 cut(s)	906, 1995, 2804, 3428, 3522, 3636
RsaNI	GTAC	6 cut(s)	905, 1994, 2803, 3427, 3521, 3635
RseI	CAYNNNNRTG	3 cut(s)	836, 1602, 3591
SacI	GAGCTC	2 cut(s)	31, 1990
SalI	GTCGAC	1 cut(s)	2486
Sau96I	GGNCC	4 cut(s)	619, 620, 1230, 2875
ScaI	AGTACT	2 cut(s)	2804, 3636
SchI	GAGTC	4 cut(s)	29, 973, 1448, 3638
ScrFI	CCNGG	6 cut(s)	289, 303, 372, 624, 691, 2007
SduI	GDGCHC	8 cut(s)	31, 295, 623, 1127, 1596, 1990, 2275, 3661
SfcI	CTRYAG	5 cut(s)	1962, 2130, 2287, 3174, 3732
SfuI	TTCGAA	3 cut(s)	359, 1415, 3888
SgrAI	CRCCGGYG	1 cut(s)	7
SmiMI	CAYNNNNRTG	3 cut(s)	836, 1602, 3591
SmlI	CTYRAG	1 cut(s)	2867
SmoI	CTYRAG	1 cut(s)	2867
SpeI	ACTAGT	2 cut(s)	860, 3274
SseBI	AGGCCT	2 cut(s)	249, 3479
SspI	AATATT	2 cut(s)	613, 1412
SstI	GAGCTC	2 cut(s)	31, 1990
StuI	AGGCCT	2 cut(s)	249, 3479
StyD4I	CCNGG	6 cut(s)	287, 301, 370, 622, 689, 2005
StyI	CCWWGG	5 cut(s)	202, 2221, 2419, 2731, 3417
TaaI	ACNGT	3 cut(s)	2297, 2408, 3663
TatI	WGTACW	5 cut(s)	904, 2802, 3426, 3520, 3634
TauI	GCSGC	1 cut(s)	810
TscAI	CASTG	7 cut(s)	430, 838, 1566, 1705, 2280, 3712, 3721
TseFI	GTSAC	2 cut(s)	164, 1700
Tsp45I	GTSAC	2 cut(s)	164, 1700
TspGWI	ACGGA	2 cut(s)	316, 1123
TspRI	CASTG	7 cut(s)	430, 838, 1566, 1705, 2280, 3712, 3721
Van91I	CCANNNNNTGG	2 cut(s)	766, 1982
VneI	GTGCAC	1 cut(s)	3657
XagI	CCTNNNNNAGG	2 cut(s)	1632, 2423
XapI	RAATTY	9 cut(s)	107, 322, 338, 361, 1091, 1196, 1423, 2325, 3890
XceI	RCATGY	2 cut(s)	2148, 2314
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1298, 2228, 3878
XmiI	GTMKAC	2 cut(s)	1689, 2487
XmnI	GAANNNNTTC	1 cut(s)	3012
ZraI	GACGTC	1 cut(s)	1277
ZrmI	AGTACT	2 cut(s)	2804, 3636

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.