AT1G14310

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007289
Physical Location & Seq
Forward (+)
4884951 .. 4886793
1843 bp
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UTR
Exon/CDS
Intron
AT1G14310.1

Sequence Viewer

Length: 765 bp
ATGGAAGCTTGTTTGAGATGTTCACGAGGAAGCTATCTAATTGAAGCCATGAGACCACTCTCCAGCTCTCTCCGGCCTTCTTCTTCTGTCTCTTTTAGCACCGGCAAGCCAATCAAGAGAGCTTATGATGGATTGTTGCTTGATGCTGGAGGAACTCTTTTGCAATTGTCGAAACCTGTTCATGAAACCTACGCTTCCTTGGGCCAAAAATATGGTCTCAAGACAACTCCAGCTGAGATCAAGGAAGGTTTCAAGAGAGTTTTTTCTGCCCCTTGGCCTGAGAAGTTACGTTACCAGGGAGATGGAAGACCATTCTGGAAGCTTGTTGTTTCGGAAGCTACTGGTTGTTCTGATAATGACTACTTCGAAGACGTCTACCAATATTATGCGAATGGCGAGGCATGGCATCTCCCAGAAGGAGCTTATGAGACAATGTCACTACTGAAAGATGCTGGAGTTAAGATGGCTGTTGTGTCTAATTTCGATACCAGGCTAAGAAAGCTATTGAAGGATCTTAATGTCATAGATATGTTCGATGCTGTGATTGTATCCGCAGAAGTCGGATACGAGAAGCCTGATGAACGAATCTTCAAATCTGCGTTAGAACAAATCAGCGTAGATGTAAACAGAGCAGTGCATGTCGGAGATGATGAAGGAGCTGATAAAGGCGGAGCAAACGCCATCGGTATTGCTTGTTGGTTGTGGGGAGAAGATGTGCAGACATTTTCGGACATACAAAAGCGGATCTTGGTTTCAGAACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2

Protein Analysis

254

Amino Acids

28.18

Weight (kDa)

5.26

Isoelectric Point (pI)

37.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 42 - 229 2.1e-19 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 43 - 232 1.9e-15 Haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 375
AccI GTMKAC 1 cut(s) 375
AciI CCGC 3 cut(s) 552, 669, 742
AclWI GGATC 2 cut(s) 519, 752
AcyI GRCGYC 1 cut(s) 372
AgsI TTSAA 4 cut(s) 44, 253, 508, 592
AjnI CCWGG 2 cut(s) 294, 488
Alw26I GTCTC 4 cut(s) 46, 94, 221, 422
AlwI GGATC 2 cut(s) 519, 752
AoxI GGCC 3 cut(s) 74, 202, 275
ArsI GACNNNNNNTTYG 2 cut(s) 199, 231
AspS9I GGNCC 1 cut(s) 202
AsuII TTCGAA 1 cut(s) 366
BarI GAAGNNNNNNTAC 2 cut(s) 275, 307
BauI CACGAG 1 cut(s) 24
BbsI GAAGAC 2 cut(s) 313, 375
BccI CCATC 4 cut(s) 122, 296, 457, 689
BciT130I CCWGG 2 cut(s) 296, 490
BciVI GTATCC 2 cut(s) 557, 559
BcoDI GTCTC 4 cut(s) 46, 94, 221, 422
BfuI GTATCC 2 cut(s) 557, 559
Bme1390I CCNGG 2 cut(s) 296, 490
BmgT120I GGNCC 1 cut(s) 202
BmrFI CCNGG 2 cut(s) 296, 490
BmsI GCATC 4 cut(s) 133, 415, 439, 526
BpiI GAAGAC 2 cut(s) 313, 375
BpmI CTGGAG 4 cut(s) 46, 168, 213, 474
Bpu14I TTCGAA 1 cut(s) 366
BpuEI CTTGAG 1 cut(s) 203
BsaHI GRCGYC 1 cut(s) 372
BsaI GGTCTC 2 cut(s) 46, 221
BsaJI CCNNGG 3 cut(s) 198, 272, 295
BsaXI ACNNNNNCTCC 2 cut(s) 699, 729
Bse118I RCCGGY 1 cut(s) 101
Bse1I ACTGG 1 cut(s) 346
BseBI CCWGG 2 cut(s) 296, 490
BseDI CCNNGG 3 cut(s) 198, 272, 295
BseMII CTCAG 2 cut(s) 225, 270
BseNI ACTGG 1 cut(s) 346
BsgI GTGCAG 1 cut(s) 737
BshFI GGCC 3 cut(s) 76, 204, 277
BsiSI CCGG 2 cut(s) 73, 102
BsmAI GTCTC 4 cut(s) 46, 94, 221, 422
BsnI GGCC 3 cut(s) 76, 204, 277
Bso31I GGTCTC 2 cut(s) 46, 221
Bsp119I TTCGAA 1 cut(s) 366
Bsp143I GATC 3 cut(s) 237, 511, 744
BspACI CCGC 3 cut(s) 552, 669, 742
BspANI GGCC 3 cut(s) 76, 204, 277
BspCNI CTCAG 2 cut(s) 226, 271
BspHI TCATGA 1 cut(s) 181
BspPI GGATC 2 cut(s) 519, 752
BspT104I TTCGAA 1 cut(s) 366
BspTNI GGTCTC 2 cut(s) 46, 221
BsrFI RCCGGY 1 cut(s) 101
BsrI ACTGG 1 cut(s) 346
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 3 cut(s) 198, 272, 295
BssMI GATC 3 cut(s) 237, 511, 744
BssNI GRCGYC 1 cut(s) 372
BssSI CACGAG 1 cut(s) 24
BssT1I CCWWGG 2 cut(s) 198, 272
Bst2BI CACGAG 1 cut(s) 24
Bst2UI CCWGG 2 cut(s) 296, 490
BstACI GRCGYC 1 cut(s) 372
BstBI TTCGAA 1 cut(s) 366
BstC8I GCNNGC 1 cut(s) 107
BstDEI CTNAG 3 cut(s) 234, 279, 494
BstKTI GATC 3 cut(s) 240, 514, 747
BstMAI GTCTC 4 cut(s) 46, 94, 221, 422
BstMBI GATC 3 cut(s) 237, 511, 744
BstMWI GCNNNNNNNGC 1 cut(s) 499
BstNI CCWGG 2 cut(s) 296, 490
BstNSI RCATGY 1 cut(s) 641
BstSCI CCNGG 2 cut(s) 294, 488
BstV2I GAAGAC 2 cut(s) 313, 375
BstX2I RGATCY 2 cut(s) 511, 744
BstXI CCANNNNNNTGG 2 cut(s) 212, 302
BstYI RGATCY 2 cut(s) 511, 744
BsuI GTATCC 2 cut(s) 557, 559
BsuRI GGCC 3 cut(s) 76, 204, 277
BtsI GCAGTG 1 cut(s) 639
BtsIMutI CAGTG 1 cut(s) 639
Cac8I GCNNGC 1 cut(s) 107
CciI TCATGA 1 cut(s) 181
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 1 cut(s) 202
CviAII CATG 4 cut(s) 49, 182, 402, 638
DdeI CTNAG 3 cut(s) 234, 279, 494
DpnI GATC 3 cut(s) 239, 513, 746
DpnII GATC 3 cut(s) 237, 511, 744
EciI GGCGGA 1 cut(s) 684
Eco130I CCWWGG 2 cut(s) 198, 272
Eco31I GGTCTC 2 cut(s) 46, 221
EcoRII CCWGG 2 cut(s) 294, 488
EcoT14I CCWWGG 2 cut(s) 198, 272
ErhI CCWWGG 2 cut(s) 198, 272
FaeI CATG 4 cut(s) 52, 185, 405, 641
FalI AAGNNNNNCTT 2 cut(s) 731, 763
FatI CATG 4 cut(s) 48, 181, 401, 637
FblI GTMKAC 1 cut(s) 375
GsuI CTGGAG 4 cut(s) 46, 168, 213, 474
HaeIII GGCC 3 cut(s) 76, 204, 277
HapII CCGG 2 cut(s) 73, 102
Hin1I GRCGYC 1 cut(s) 372
Hin1II CATG 4 cut(s) 52, 185, 405, 641
HindIII AAGCTT 2 cut(s) 6, 320
HinfI GANTC 1 cut(s) 585
HpaII CCGG 2 cut(s) 73, 102
Hpy166II GTNNAC 3 cut(s) 23, 376, 625
Hpy188I TCNGA 6 cut(s) 334, 352, 563, 644, 730, 757
Hpy188III TCNNGA 6 cut(s) 24, 115, 182, 220, 253, 316
Hpy8I GTNNAC 3 cut(s) 23, 376, 625
HpyAV CCTTC 5 cut(s) 87, 239, 410, 502, 647
HpyCH4IV ACGT 2 cut(s) 289, 372
HpyCH4V TGCA 3 cut(s) 163, 637, 718
HpyF10VI GCNNNNNNNGC 1 cut(s) 499
HpyF3I CTNAG 3 cut(s) 234, 279, 494
HpySE526I ACGT 2 cut(s) 289, 372
Hsp92I GRCGYC 1 cut(s) 372
Hsp92II CATG 4 cut(s) 52, 185, 405, 641
Kzo9I GATC 3 cut(s) 237, 511, 744
LmnI GCTCC 3 cut(s) 419, 656, 671
LweI GCATC 4 cut(s) 133, 415, 439, 526
MaeII ACGT 2 cut(s) 289, 372
MaeIII GTNAC 3 cut(s) 285, 290, 435
MalI GATC 3 cut(s) 239, 513, 746
MboI GATC 3 cut(s) 237, 511, 744
MboII GAAGA 6 cut(s) 72, 75, 318, 380, 580, 722
MfeI CAATTG 1 cut(s) 164
MflI RGATCY 2 cut(s) 511, 744
MluCI AATT 3 cut(s) 39, 164, 478
MmeI TCCRAC 2 cut(s) 541, 622
MnlI CCTC 3 cut(s) 20, 143, 391
MseI TTAA 2 cut(s) 459, 516
MslI CAYNNNNRTG 1 cut(s) 527
MspA1I CMGCKG 1 cut(s) 233
MspI CCGG 2 cut(s) 73, 102
MspR9I CCNGG 2 cut(s) 296, 490
MunI CAATTG 1 cut(s) 164
MvaI CCWGG 2 cut(s) 296, 490
MwoI GCNNNNNNNGC 1 cut(s) 499
NdeII GATC 3 cut(s) 237, 511, 744
NlaIII CATG 4 cut(s) 52, 185, 405, 641
NmuCI GTSAC 1 cut(s) 435
NspI RCATGY 1 cut(s) 641
NspV TTCGAA 1 cut(s) 366
PagI TCATGA 1 cut(s) 181
PfeI GAWTC 1 cut(s) 585
PflFI GACNNNGTC 1 cut(s) 433
Psp6I CCWGG 2 cut(s) 294, 488
PspGI CCWGG 2 cut(s) 294, 488
PspPI GGNCC 1 cut(s) 202
PsuI RGATCY 2 cut(s) 511, 744
PsyI GACNNNGTC 1 cut(s) 433
PvuII CAGCTG 1 cut(s) 233
RseI CAYNNNNRTG 1 cut(s) 527
SaqAI TTAA 2 cut(s) 459, 516
Sau3AI GATC 3 cut(s) 237, 511, 744
Sau96I GGNCC 1 cut(s) 202
ScrFI CCNGG 2 cut(s) 296, 490
SfaNI GCATC 4 cut(s) 133, 415, 439, 526
SfuI TTCGAA 1 cut(s) 366
SmiMI CAYNNNNRTG 1 cut(s) 527
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
Sse9I AATT 3 cut(s) 39, 164, 478
SsiI CCGC 3 cut(s) 552, 669, 742
SspI AATATT 1 cut(s) 383
StyD4I CCNGG 2 cut(s) 294, 488
StyI CCWWGG 2 cut(s) 198, 272
TaiI ACGT 2 cut(s) 292, 375
TaqI TCGA 4 cut(s) 170, 366, 483, 534
TasI AATT 3 cut(s) 39, 164, 478
TfiI GAWTC 1 cut(s) 585
Tru1I TTAA 2 cut(s) 459, 516
Tru9I TTAA 2 cut(s) 459, 516
TscAI CASTG 1 cut(s) 639
TseFI GTSAC 1 cut(s) 435
Tsp45I GTSAC 1 cut(s) 435
TspDTI ATGAA 4 cut(s) 170, 198, 594, 666
TspRI CASTG 1 cut(s) 639
Tth111I GACNNNGTC 1 cut(s) 433
XceI RCATGY 1 cut(s) 641
XmiI GTMKAC 1 cut(s) 375
ZraI GACGTC 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.