MD13G1073200.v1.1

Haloacid dehalogenase-like hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007289
Physical Location & Seq
Forward (+)
5156375 .. 5158356
1982 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1073200.v1.1.491

Sequence Viewer

Length: 795 bp
ATGGAAGGTTGCTTGATGAGATGCTCTCACGGCGGCGCACTATTCAAAAACCTCAAACCCCTCCCTTTAAACCTCCCCAGAAACTCCATTCGCGGCTCTTCCATGTCCGTCTACTCCGGCGGAGGAGGAGGATCGGCTCTTAGTCTCAAGGTCTACGACGCATTGCTGTTAGACGCCGGAGGCACTCTGTTGCAGTTGGCGAAGCCAGTCGAAGATGTCTACGCTTCCATTGGAACCAAATACGGTGTGACTGCAACTCCAGCTGAAATCAAGCAGGGATTCAGAAGAGCACTTTCTGCTCCTTGGCCTCAGAAGCTGCGTTACCAGGGAGATGGGAGGCCATTTTGGAAACTTGTTGTGTCCGAAGCCACTGGTTGTTCTAATCTCGATTATTTCGAACAAGTTTATGAGTACTATGCTAAAGGCGACGCATGGCGTCTTCCAGATGGAGCTTATGAAACAATGCTTCTTCTCAAAGAGGCTGGAGTTAAGCTGGCTGTTGTATCCAATTTCGACTCCCGCTTACGGAAGCTACTTGAGGACCTTAATGTTTTGCATCTGTTTGATTCCGTTATCATATCTTCAGAAGTTGGGCATGAGAAACCAGATGCAGGCATATTCGAAGCCGCTTTAGATCAAGTACGTGTGGATGCTGGCAAAGCAGTACATGTTGGCGACGACAAAATTGCAGATAAGGATGGGGCCAATGCCATTGGAATTGATTGCTGGTTATGGGGCCTAGATGTGAAGGCATTCTCTGACATTCAAAATCGCATTCTCATTTCAGAGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2

Protein Analysis

265

Amino Acids

28.7

Weight (kDa)

5.64

Isoelectric Point (pI)

28.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 52 - 239 2.3e-20 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 130 - 242 2.3e-15 Haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 111, 153, 219
AccII CGCG 1 cut(s) 93
AciI CCGC 5 cut(s) 33, 93, 120, 520, 627
AclWI GGATC 1 cut(s) 139
AcuI CTGAAG 1 cut(s) 567
AcyI GRCGYC 2 cut(s) 174, 436
AfaI GTAC 3 cut(s) 413, 642, 666
AfiI CCNNNNNNNGG 2 cut(s) 525, 611
AflIII ACRYGT 2 cut(s) 643, 667
AgsI TTSAA 2 cut(s) 46, 767
AjnI CCWGG 1 cut(s) 324
AluBI AGCT 5 cut(s) 263, 316, 452, 493, 532
AluI AGCT 5 cut(s) 263, 316, 452, 493, 532
Alw21I GWGCWC 1 cut(s) 292
Alw26I GTCTC 1 cut(s) 149
AlwI GGATC 1 cut(s) 139
AlwNI CAGNNNCTG 1 cut(s) 316
AoxI GGCC 4 cut(s) 305, 338, 702, 736
ApeKI GCWGC 1 cut(s) 316
Asp700I GAANNNNTTC 1 cut(s) 752
AspLEI GCGC 1 cut(s) 38
AspS9I GGNCC 3 cut(s) 541, 702, 736
AsuII TTCGAA 2 cut(s) 396, 621
AvaII GGWCC 1 cut(s) 541
BarI GAAGNNNNNNTAC 2 cut(s) 305, 337
BbsI GAAGAC 1 cut(s) 431
Bbv12I GWGCWC 1 cut(s) 292
BbvI GCAGC 1 cut(s) 303
BccI CCATC 3 cut(s) 326, 440, 692
BceAI ACGGC 1 cut(s) 46
BcgI CGANNNNNNTGC 2 cut(s) 668, 702
BciT130I CCWGG 1 cut(s) 326
BciVI GTATCC 1 cut(s) 514
BcoDI GTCTC 1 cut(s) 149
BfaI CTAG 1 cut(s) 740
BfuI GTATCC 1 cut(s) 514
BisI GCNGC 4 cut(s) 34, 94, 317, 627
BlsI GCNGC 4 cut(s) 35, 95, 318, 628
BmcAI AGTACT 1 cut(s) 413
Bme1390I CCNGG 1 cut(s) 326
Bme18I GGWCC 1 cut(s) 541
BmgT120I GGNCC 3 cut(s) 541, 702, 736
BmiI GGNNCC 3 cut(s) 235, 703, 737
BmrFI CCNGG 1 cut(s) 326
BmsI GCATC 4 cut(s) 11, 565, 598, 640
BpiI GAAGAC 1 cut(s) 431
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpmI CTGGAG 2 cut(s) 243, 504
Bpu14I TTCGAA 2 cut(s) 396, 621
BpuEI CTTGAG 2 cut(s) 131, 557
BsaAI YACGTR 1 cut(s) 644
BsaHI GRCGYC 2 cut(s) 174, 436
BsaJI CCNNGG 2 cut(s) 302, 325
BsaXI ACNNNNNCTCC 2 cut(s) 241, 271
Bsc4I CCNNNNNNNGG 2 cut(s) 525, 611
Bse1I ACTGG 2 cut(s) 206, 376
Bse3DI GCAATG 1 cut(s) 161
BseBI CCWGG 1 cut(s) 326
BseDI CCNNGG 2 cut(s) 302, 325
BseGI GGATG 2 cut(s) 655, 703
BseLI CCNNNNNNNGG 2 cut(s) 525, 611
BseMI GCAATG 1 cut(s) 161
BseMII CTCAG 1 cut(s) 323
BseNI ACTGG 2 cut(s) 206, 376
BseRI GAGGAG 2 cut(s) 138, 141
BseXI GCAGC 1 cut(s) 303
Bsh1236I CGCG 1 cut(s) 93
BshFI GGCC 4 cut(s) 307, 340, 704, 738
BsiHKAI GWGCWC 1 cut(s) 292
BsiSI CCGG 2 cut(s) 117, 177
BslI CCNNNNNNNGG 2 cut(s) 525, 611
BsmAI GTCTC 1 cut(s) 149
BsmI GAATGC 2 cut(s) 752, 774
BsnI GGCC 4 cut(s) 307, 340, 704, 738
Bsp119I TTCGAA 2 cut(s) 396, 621
Bsp1286I GDGCHC 1 cut(s) 292
Bsp143I GATC 2 cut(s) 131, 634
BspACI CCGC 5 cut(s) 33, 93, 120, 520, 627
BspANI GGCC 4 cut(s) 307, 340, 704, 738
BspCNI CTCAG 1 cut(s) 322
BspFNI CGCG 1 cut(s) 93
BspLI GGNNCC 3 cut(s) 235, 703, 737
BspPI GGATC 1 cut(s) 139
BspQI GCTCTTC 2 cut(s) 103, 280
BspT104I TTCGAA 2 cut(s) 396, 621
BsrDI GCAATG 1 cut(s) 161
BsrI ACTGG 2 cut(s) 206, 376
BssECI CCNNGG 2 cut(s) 302, 325
BssMI GATC 2 cut(s) 131, 634
BssNI GRCGYC 2 cut(s) 174, 436
BssT1I CCWWGG 1 cut(s) 302
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 1 cut(s) 245
Bst6I CTCTTC 2 cut(s) 103, 280
BstACI GRCGYC 2 cut(s) 174, 436
BstAPI GCANNNNNTGC 1 cut(s) 296
BstBAI YACGTR 1 cut(s) 644
BstBI TTCGAA 2 cut(s) 396, 621
BstC8I GCNNGC 3 cut(s) 495, 613, 655
BstDEI CTNAG 3 cut(s) 140, 309, 792
BstF5I GGATG 2 cut(s) 655, 703
BstFNI CGCG 1 cut(s) 93
BstHHI GCGC 1 cut(s) 38
BstKTI GATC 2 cut(s) 134, 637
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 2 cut(s) 131, 634
BstMWI GCNNNNNNNGC 5 cut(s) 30, 260, 296, 313, 659
BstNI CCWGG 1 cut(s) 326
BstNSI RCATGY 1 cut(s) 671
BstSCI CCNGG 1 cut(s) 324
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 1 cut(s) 303
BstV2I GAAGAC 1 cut(s) 431
BstXI CCANNNNNNTGG 1 cut(s) 332
BsuI GTATCC 1 cut(s) 514
BsuRI GGCC 4 cut(s) 307, 340, 704, 738
BtsCI GGATG 2 cut(s) 655, 703
BtsIMutI CAGTG 1 cut(s) 369
Cac8I GCNNGC 3 cut(s) 495, 613, 655
CaiI CAGNNNCTG 1 cut(s) 316
CfoI GCGC 1 cut(s) 38
Cfr13I GGNCC 3 cut(s) 541, 702, 736
CseI GACGC 4 cut(s) 167, 182, 425, 437
Csp6I GTAC 3 cut(s) 412, 641, 665
CviAII CATG 4 cut(s) 103, 432, 596, 668
CviQI GTAC 3 cut(s) 412, 641, 665
DdeI CTNAG 3 cut(s) 140, 309, 792
DpnI GATC 2 cut(s) 133, 636
DpnII GATC 2 cut(s) 131, 634
DraI TTTAAA 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 103, 280
EarI CTCTTC 2 cut(s) 103, 280
EciI GGCGGA 1 cut(s) 135
Eco130I CCWWGG 1 cut(s) 302
Eco47I GGWCC 1 cut(s) 541
Eco57I CTGAAG 1 cut(s) 567
EcoO109I RGGNCCY 2 cut(s) 541, 736
EcoRII CCWGG 1 cut(s) 324
EcoT14I CCWWGG 1 cut(s) 302
ErhI CCWWGG 1 cut(s) 302
FaeI CATG 4 cut(s) 106, 435, 599, 671
FatI CATG 4 cut(s) 102, 431, 595, 667
FauI CCCGC 1 cut(s) 527
FblI GTMKAC 3 cut(s) 111, 153, 219
Fnu4HI GCNGC 4 cut(s) 34, 94, 317, 627
FokI GGATG 2 cut(s) 662, 710
Fsp4HI GCNGC 4 cut(s) 34, 94, 317, 627
FspBI CTAG 1 cut(s) 740
GlaI GCGC 1 cut(s) 37
GluI GCNGC 4 cut(s) 34, 94, 317, 627
GsuI CTGGAG 2 cut(s) 243, 504
HaeIII GGCC 4 cut(s) 307, 340, 704, 738
HapII CCGG 2 cut(s) 117, 177
HgaI GACGC 4 cut(s) 167, 182, 425, 437
HhaI GCGC 1 cut(s) 38
Hin1I GRCGYC 2 cut(s) 174, 436
Hin1II CATG 4 cut(s) 106, 435, 599, 671
Hin6I GCGC 1 cut(s) 36
HinP1I GCGC 1 cut(s) 36
HinfI GANTC 4 cut(s) 279, 515, 566, 788
HpaII CCGG 2 cut(s) 117, 177
Hpy166II GTNNAC 3 cut(s) 112, 154, 220
Hpy188I TCNGA 6 cut(s) 284, 312, 364, 586, 760, 787
Hpy188III TCNNGA 2 cut(s) 386, 443
Hpy8I GTNNAC 3 cut(s) 112, 154, 220
Hpy99I CGWCG 3 cut(s) 161, 431, 680
HpyAV CCTTC 1 cut(s) 742
HpyCH4III ACNGT 1 cut(s) 245
HpyCH4IV ACGT 1 cut(s) 643
HpyCH4V TGCA 5 cut(s) 193, 254, 556, 611, 689
HpyF10VI GCNNNNNNNGC 5 cut(s) 30, 260, 296, 313, 659
HpyF3I CTNAG 3 cut(s) 140, 309, 792
HpySE526I ACGT 1 cut(s) 643
Hsp92I GRCGYC 2 cut(s) 174, 436
Hsp92II CATG 4 cut(s) 106, 435, 599, 671
HspAI GCGC 1 cut(s) 36
Kzo9I GATC 2 cut(s) 131, 634
LguI GCTCTTC 2 cut(s) 103, 280
LmnI GCTCC 2 cut(s) 304, 449
Lsp1109I GCAGC 1 cut(s) 303
LweI GCATC 4 cut(s) 11, 565, 598, 640
MaeI CTAG 1 cut(s) 740
MaeII ACGT 1 cut(s) 643
MaeIII GTNAC 2 cut(s) 247, 320
MalI GATC 2 cut(s) 133, 636
MboI GATC 2 cut(s) 131, 634
MboII GAAGA 6 cut(s) 90, 224, 297, 431, 461, 573
MhlI GDGCHC 1 cut(s) 292
MluCI AATT 3 cut(s) 508, 684, 717
MlyI GAGTC 1 cut(s) 509
MroXI GAANNNNTTC 1 cut(s) 752
MseI TTAA 3 cut(s) 68, 489, 546
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 2 cut(s) 117, 177
MspR9I CCNGG 1 cut(s) 326
Mva1269I GAATGC 2 cut(s) 752, 774
MvaI CCWGG 1 cut(s) 326
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 5 cut(s) 30, 260, 296, 313, 659
NdeII GATC 2 cut(s) 131, 634
NlaIII CATG 4 cut(s) 106, 435, 599, 671
NlaIV GGNNCC 3 cut(s) 235, 703, 737
NmuCI GTSAC 1 cut(s) 247
NspI RCATGY 1 cut(s) 671
NspV TTCGAA 2 cut(s) 396, 621
PciI ACATGT 1 cut(s) 667
PciSI GCTCTTC 2 cut(s) 103, 280
PcsI WCGNNNNNNNCGW 1 cut(s) 393
PctI GAATGC 2 cut(s) 752, 774
PdmI GAANNNNTTC 1 cut(s) 752
PfeI GAWTC 2 cut(s) 279, 566
PkrI GCNGC 4 cut(s) 35, 95, 318, 628
PleI GAGTC 1 cut(s) 509
PpsI GAGTC 1 cut(s) 509
Ppu21I YACGTR 1 cut(s) 644
PpuMI RGGWCCY 1 cut(s) 541
PscI ACATGT 1 cut(s) 667
Psp5II RGGWCCY 1 cut(s) 541
Psp6I CCWGG 1 cut(s) 324
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 3 cut(s) 235, 703, 737
PspPI GGNCC 3 cut(s) 541, 702, 736
PspPPI RGGWCCY 1 cut(s) 541
PstNI CAGNNNCTG 1 cut(s) 316
PvuII CAGCTG 1 cut(s) 263
RsaI GTAC 3 cut(s) 413, 642, 666
RsaNI GTAC 3 cut(s) 412, 641, 665
SapI GCTCTTC 2 cut(s) 103, 280
SaqAI TTAA 3 cut(s) 68, 489, 546
SatI GCNGC 4 cut(s) 34, 94, 317, 627
Sau3AI GATC 2 cut(s) 131, 634
Sau96I GGNCC 3 cut(s) 541, 702, 736
ScaI AGTACT 1 cut(s) 413
SchI GAGTC 1 cut(s) 509
ScrFI CCNGG 1 cut(s) 326
SduI GDGCHC 1 cut(s) 292
SfaNI GCATC 4 cut(s) 11, 565, 598, 640
SfuI TTCGAA 2 cut(s) 396, 621
SinI GGWCC 1 cut(s) 541
SmlI CTYRAG 2 cut(s) 146, 536
SmoI CTYRAG 2 cut(s) 146, 536
Sse9I AATT 3 cut(s) 508, 684, 717
SsiI CCGC 5 cut(s) 33, 93, 120, 520, 627
SspMI CTAG 1 cut(s) 740
StyD4I CCNGG 1 cut(s) 324
StyI CCWWGG 1 cut(s) 302
TaaI ACNGT 1 cut(s) 245
TaiI ACGT 1 cut(s) 646
TaqI TCGA 5 cut(s) 210, 387, 396, 513, 621
TasI AATT 3 cut(s) 508, 684, 717
TatI WGTACW 2 cut(s) 411, 664
TauI GCSGC 3 cut(s) 36, 96, 629
TfiI GAWTC 2 cut(s) 279, 566
Tru1I TTAA 3 cut(s) 68, 489, 546
Tru9I TTAA 3 cut(s) 68, 489, 546
TscAI CASTG 1 cut(s) 376
TseFI GTSAC 1 cut(s) 247
TseI GCWGC 1 cut(s) 316
Tsp45I GTSAC 1 cut(s) 247
TspDTI ATGAA 1 cut(s) 471
TspGWI ACGGA 3 cut(s) 97, 541, 559
TspRI CASTG 1 cut(s) 376
VpaK11BI GGWCC 1 cut(s) 541
XceI RCATGY 1 cut(s) 671
XmiI GTMKAC 3 cut(s) 111, 153, 219
XmnI GAANNNNTTC 1 cut(s) 752
XspI CTAG 1 cut(s) 740
ZrmI AGTACT 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.