MD05G1042500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007289
Physical Location & Seq
Forward (+)
7169301 .. 7170013
713 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1042500.v1.1.491

Sequence Viewer

Length: 417 bp
ATGCCACGGTCTCTAGAAGGTTGCCATGGAAGGTTGCTTGATGAGATGGTCTCAAGGCAGCGCTCTTCTAAAAGCCCTCAAACCTCTCGTTGCATGCTTCTTCTTCGCATGCGTCCTTTTTTGCTTTCTTCTCCGCATCGTGGCTTTTGCCCGTTGATTACTTCATTTTTCAGGCTCTATTTTTCTGGGTCCTTATCAGAATTTTGTGCCATTGTTTGTTCGCAGAGTGAGGCTCAATGGGGAGTAATGGCTAACAAGAATCTGGAGAAGTTGGTGTCCATTGACGCGCAGCTTCAGCTTCTGGTTCCAGTTAAGTTGATTGGGTTCTGGGTTGGGTGTTTTGCATTCTTTCTCTCTGTGAAGGGAGGCTGGATTTGTGAGTTTTCTGGGATGCCAATTAGTTCTGATCAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

139

Amino Acids

15.52

Weight (kDa)

8.99

Isoelectric Point (pI)

58.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 287
AciI CCGC 1 cut(s) 134
AcsI RAATTY 2 cut(s) 200, 411
AcuI CTGAAG 1 cut(s) 278
AfeI AGCGCT 1 cut(s) 62
AfiI CCNNNNNNNGG 1 cut(s) 140
AluBI AGCT 2 cut(s) 292, 298
AluI AGCT 2 cut(s) 292, 298
Alw26I GTCTC 2 cut(s) 15, 55
AlwNI CAGNNNCTG 1 cut(s) 301
Aor51HI AGCGCT 1 cut(s) 62
ApeKI GCWGC 2 cut(s) 58, 289
ApoI RAATTY 2 cut(s) 200, 411
AspLEI GCGC 2 cut(s) 63, 289
AspS9I GGNCC 1 cut(s) 189
AvaII GGWCC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 70, 301
BccI CCATC 1 cut(s) 40
BclI TGATCA 1 cut(s) 406
BcoDI GTCTC 2 cut(s) 15, 55
BfaI CTAG 1 cut(s) 14
BfoI RGCGCY 1 cut(s) 64
BisI GCNGC 2 cut(s) 59, 290
BlsI GCNGC 2 cut(s) 60, 291
Bme18I GGWCC 1 cut(s) 189
BmgT120I GGNCC 1 cut(s) 189
BmiI GGNNCC 2 cut(s) 190, 306
BmsI GCATC 2 cut(s) 145, 381
BplI GAGNNNNNCTC 4 cut(s) 35, 67, 217, 249
BpmI CTGGAG 1 cut(s) 284
BpuEI CTTGAG 1 cut(s) 37
BsaI GGTCTC 2 cut(s) 15, 55
BsaJI CCNNGG 2 cut(s) 5, 25
Bsc4I CCNNNNNNNGG 1 cut(s) 140
Bse1I ACTGG 1 cut(s) 308
BseDI CCNNGG 2 cut(s) 5, 25
BseGI GGATG 1 cut(s) 396
BseLI CCNNNNNNNGG 1 cut(s) 140
BseNI ACTGG 1 cut(s) 308
BseXI GCAGC 2 cut(s) 70, 301
Bsh1236I CGCG 1 cut(s) 287
BslI CCNNNNNNNGG 1 cut(s) 140
BsmAI GTCTC 2 cut(s) 15, 55
BsmI GAATGC 1 cut(s) 344
Bso31I GGTCTC 2 cut(s) 15, 55
Bsp143I GATC 1 cut(s) 406
Bsp19I CCATGG 1 cut(s) 25
BspACI CCGC 1 cut(s) 134
BspFNI CGCG 1 cut(s) 287
BspLI GGNNCC 2 cut(s) 190, 306
BspQI GCTCTTC 1 cut(s) 70
BspTNI GGTCTC 2 cut(s) 15, 55
BsrI ACTGG 1 cut(s) 308
BssECI CCNNGG 2 cut(s) 5, 25
BssMI GATC 1 cut(s) 406
BssT1I CCWWGG 1 cut(s) 25
Bst4CI ACNGT 1 cut(s) 9
Bst6I CTCTTC 1 cut(s) 70
BstC8I GCNNGC 2 cut(s) 95, 110
BstDSI CCRYGG 2 cut(s) 5, 25
BstF5I GGATG 1 cut(s) 396
BstFNI CGCG 1 cut(s) 287
BstH2I RGCGCY 1 cut(s) 64
BstHHI GCGC 2 cut(s) 63, 289
BstKTI GATC 1 cut(s) 409
BstMAI GTCTC 2 cut(s) 15, 55
BstMBI GATC 1 cut(s) 406
BstMWI GCNNNNNNNGC 1 cut(s) 295
BstNSI RCATGY 2 cut(s) 97, 112
BstUI CGCG 1 cut(s) 287
BstV1I GCAGC 2 cut(s) 70, 301
BtgI CCRYGG 2 cut(s) 5, 25
BtsCI GGATG 1 cut(s) 396
Cac8I GCNNGC 2 cut(s) 95, 110
CaiI CAGNNNCTG 1 cut(s) 301
CfoI GCGC 2 cut(s) 63, 289
Cfr13I GGNCC 1 cut(s) 189
CseI GACGC 2 cut(s) 101, 293
CviAII CATG 3 cut(s) 26, 94, 109
CviJI RGCY 8 cut(s) 75, 144, 175, 233, 251, 292, 298, 369
CviKI_1 RGCY 8 cut(s) 75, 144, 175, 233, 251, 292, 298, 369
DpnI GATC 1 cut(s) 408
DpnII GATC 1 cut(s) 406
Eam1104I CTCTTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 70
Eco130I CCWWGG 1 cut(s) 25
Eco31I GGTCTC 2 cut(s) 15, 55
Eco47I GGWCC 1 cut(s) 189
Eco47III AGCGCT 1 cut(s) 62
Eco57I CTGAAG 1 cut(s) 278
EcoO109I RGGNCCY 1 cut(s) 189
EcoT14I CCWWGG 1 cut(s) 25
ErhI CCWWGG 1 cut(s) 25
FaeI CATG 3 cut(s) 29, 97, 112
FaiI YATR 3 cut(s) 27, 95, 110
FatI CATG 3 cut(s) 25, 93, 108
FbaI TGATCA 1 cut(s) 406
Fnu4HI GCNGC 2 cut(s) 59, 290
FokI GGATG 1 cut(s) 403
Fsp4HI GCNGC 2 cut(s) 59, 290
FspBI CTAG 1 cut(s) 14
GlaI GCGC 2 cut(s) 62, 288
GluI GCNGC 2 cut(s) 59, 290
GsuI CTGGAG 1 cut(s) 284
HaeII RGCGCY 1 cut(s) 64
HgaI GACGC 2 cut(s) 101, 293
HhaI GCGC 2 cut(s) 63, 289
Hin1II CATG 3 cut(s) 29, 97, 112
Hin6I GCGC 2 cut(s) 61, 287
HinP1I GCGC 2 cut(s) 61, 287
HinfI GANTC 1 cut(s) 259
Hpy188I TCNGA 2 cut(s) 199, 406
Hpy188III TCNNGA 2 cut(s) 14, 263
HpyAV CCTTC 3 cut(s) 11, 24, 355
HpyCH4III ACNGT 1 cut(s) 9
HpyCH4V TGCA 2 cut(s) 93, 344
HpyF10VI GCNNNNNNNGC 1 cut(s) 295
Hsp92II CATG 3 cut(s) 29, 97, 112
HspAI GCGC 2 cut(s) 61, 287
Ksp22I TGATCA 1 cut(s) 406
Kzo9I GATC 1 cut(s) 406
LguI GCTCTTC 1 cut(s) 70
LpnPI CCDG 8 cut(s) 157, 171, 248, 287, 313, 321, 355, 372
Lsp1109I GCAGC 2 cut(s) 70, 301
LweI GCATC 2 cut(s) 145, 381
MaeI CTAG 1 cut(s) 14
MalI GATC 1 cut(s) 408
MboI GATC 1 cut(s) 406
MboII GAAGA 4 cut(s) 57, 92, 95, 120
MluCI AATT 3 cut(s) 200, 396, 411
MnlI CCTC 4 cut(s) 87, 94, 223, 359
MseI TTAA 2 cut(s) 312, 415
Mva1269I GAATGC 1 cut(s) 344
MvnI CGCG 1 cut(s) 287
MwoI GCNNNNNNNGC 1 cut(s) 295
NcoI CCATGG 1 cut(s) 25
NdeII GATC 1 cut(s) 406
NlaIII CATG 3 cut(s) 29, 97, 112
NlaIV GGNNCC 2 cut(s) 190, 306
NspI RCATGY 2 cut(s) 97, 112
PaeI GCATGC 2 cut(s) 97, 112
PciSI GCTCTTC 1 cut(s) 70
PctI GAATGC 1 cut(s) 344
PfeI GAWTC 1 cut(s) 259
PkrI GCNGC 2 cut(s) 60, 291
PpuMI RGGWCCY 1 cut(s) 189
Psp5II RGGWCCY 1 cut(s) 189
PspN4I GGNNCC 2 cut(s) 190, 306
PspPI GGNCC 1 cut(s) 189
PspPPI RGGWCCY 1 cut(s) 189
PstNI CAGNNNCTG 1 cut(s) 301
SapI GCTCTTC 1 cut(s) 70
SaqAI TTAA 2 cut(s) 312, 415
SatI GCNGC 2 cut(s) 59, 290
Sau3AI GATC 1 cut(s) 406
Sau96I GGNCC 1 cut(s) 189
SetI ASST 5 cut(s) 22, 35, 86, 294, 300
SfaNI GCATC 2 cut(s) 145, 381
SinI GGWCC 1 cut(s) 189
SmlI CTYRAG 1 cut(s) 52
SmoI CTYRAG 1 cut(s) 52
SphI GCATGC 2 cut(s) 97, 112
Sse9I AATT 3 cut(s) 200, 396, 411
SsiI CCGC 1 cut(s) 134
SspMI CTAG 1 cut(s) 14
StyI CCWWGG 1 cut(s) 25
TaaI ACNGT 1 cut(s) 9
TasI AATT 3 cut(s) 200, 396, 411
TfiI GAWTC 1 cut(s) 259
Tru1I TTAA 2 cut(s) 312, 415
Tru9I TTAA 2 cut(s) 312, 415
TseI GCWGC 2 cut(s) 58, 289
TspDTI ATGAA 1 cut(s) 153
VpaK11BI GGWCC 1 cut(s) 189
XapI RAATTY 2 cut(s) 200, 411
XbaI TCTAGA 1 cut(s) 13
XceI RCATGY 2 cut(s) 97, 112
XspI CTAG 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.