RchiOBHm_Chr4g0439981

Haloacid dehalogenase-like hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0007289
Physical Location & Seq
Forward (+)
62274203 .. 62276753
2551 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40801

Sequence Viewer

Length: 792 bp
ATGGGAGCGAGACCCCTTCGCTTTGGTAAAATGGAGGGTTGCTTGGTCAAATGCTCTCACGGCGGCAGCGCTCTTTTGAAAACCCTTAAACCTCTTCATCCCAAAACCCACATTCGCTGCTTCTCTGCCCTCCGATCGCCTCGCAGAGCCTACGACGCATTGTTGTTGGACGCCGGAGGTACTCTCTTGCAGCTGGCCAACCCCGTCGAAGAGGTCTACGCCTCCATAGGAACCCAATACGGTGTGACTGCAACTCCAGCTGAAATCAAGCAGGGTTTCAGAAGAGCCTTCTCTGCTCCTTGGCCTCAAAAGCTCCGATACCAGGGAGATGGGAGGCCATTTTGGAAACTAGTTGTTTCAGAAGCCACTGGTTGTTCTGATCTTGATTATTTCGAACACGTCTATCAGTACTATGCTAGAGGTGATTCATGGCGTTTACCCCATGGAGCTTATGAAACAATGGTACTTCTCAAGGATGCTGGAGTTAAGCTTGCTGTTGTATCGAATTTTGACTCCCGCTTAAGGAAACTACTCGAGGAGCTGAATGTGTTGCATCTGTTTGATGCTGTTATCATATCTTCAGAGGTTGGTTCTGAAAAACCAGATGCAAACATATTTAAAGCTGCGTTAGATCAAACTCATGCGGATGCTGGCAAGGCAGTACATGTCGGTGATGATGAAAATGCAGATAAGATTGGGGCCAATGCTGTTGGAATTGATTGCTGGTTATGGGGCAAGGAGGTGAAGACATTCTCCGACATTCGAAATAGAATTCTCATTTCAGAGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2

Protein Analysis

263

Amino Acids

29.08

Weight (kDa)

8.21

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 51 - 238 1e-20 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 130 - 241 3.8e-15 Haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 216
AciI CCGC 3 cut(s) 63, 517, 644
AcoI YGGCCR 1 cut(s) 195
AcsI RAATTY 2 cut(s) 505, 771
AcuI CTGAAG 1 cut(s) 564
AcyI GRCGYC 1 cut(s) 171
AfaI GTAC 4 cut(s) 181, 410, 465, 663
AfeI AGCGCT 1 cut(s) 70
AfiI CCNNNNNNNGG 2 cut(s) 322, 522
AflII CTTAAG 1 cut(s) 520
AflIII ACRYGT 2 cut(s) 397, 664
AgsI TTSAA 1 cut(s) 79
AhlI ACTAGT 1 cut(s) 349
AjiI CACGTC 1 cut(s) 400
AjnI CCWGG 1 cut(s) 321
AluBI AGCT 7 cut(s) 193, 260, 313, 449, 490, 541, 623
AluI AGCT 7 cut(s) 193, 260, 313, 449, 490, 541, 623
Alw26I GTCTC 1 cut(s) 4
Ama87I CYCGRG 1 cut(s) 533
Aor51HI AGCGCT 1 cut(s) 70
AoxI GGCC 4 cut(s) 195, 302, 335, 699
ApeKI GCWGC 4 cut(s) 66, 117, 190, 623
ApoI RAATTY 2 cut(s) 505, 771
Asp700I GAANNNNTTC 1 cut(s) 749
AspLEI GCGC 1 cut(s) 71
AspS9I GGNCC 1 cut(s) 699
AsuHPI GGTGA 3 cut(s) 434, 683, 754
AsuII TTCGAA 2 cut(s) 393, 763
AvaI CYCGRG 1 cut(s) 533
BalI TGGCCA 1 cut(s) 197
BbsI GAAGAC 1 cut(s) 752
BbvI GCAGC 4 cut(s) 78, 104, 202, 610
BccI CCATC 1 cut(s) 323
BceAI ACGGC 1 cut(s) 76
BciT130I CCWGG 1 cut(s) 323
BcoDI GTCTC 1 cut(s) 4
BcuI ACTAGT 1 cut(s) 349
BfaI CTAG 2 cut(s) 350, 417
BfoI RGCGCY 1 cut(s) 72
BfrI CTTAAG 1 cut(s) 520
BisI GCNGC 5 cut(s) 64, 67, 118, 191, 624
BlsI GCNGC 5 cut(s) 65, 68, 119, 192, 625
BmcAI AGTACT 1 cut(s) 410
Bme1390I CCNGG 1 cut(s) 323
BmeT110I CYCGRG 1 cut(s) 533
BmgBI CACGTC 1 cut(s) 400
BmgT120I GGNCC 1 cut(s) 699
BmiI GGNNCC 2 cut(s) 232, 700
BmrFI CCNGG 1 cut(s) 323
BmsI GCATC 5 cut(s) 466, 553, 562, 595, 637
BpiI GAAGAC 1 cut(s) 752
BplI GAGNNNNNCTC 2 cut(s) 168, 200
BpmI CTGGAG 2 cut(s) 240, 501
Bpu14I TTCGAA 2 cut(s) 393, 763
BpuEI CTTGAG 1 cut(s) 455
BsaHI GRCGYC 1 cut(s) 171
BsaI GGTCTC 1 cut(s) 4
BsaJI CCNNGG 3 cut(s) 299, 322, 442
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bsc4I CCNNNNNNNGG 2 cut(s) 322, 522
Bse1I ACTGG 1 cut(s) 373
BseBI CCWGG 1 cut(s) 323
BseDI CCNNGG 3 cut(s) 299, 322, 442
BseGI GGATG 3 cut(s) 97, 481, 652
BseLI CCNNNNNNNGG 2 cut(s) 322, 522
BseNI ACTGG 1 cut(s) 373
BseRI GAGGAG 1 cut(s) 551
BseXI GCAGC 4 cut(s) 78, 104, 202, 610
Bsh1285I CGRYCG 1 cut(s) 137
BshFI GGCC 4 cut(s) 197, 304, 337, 701
BsiEI CGRYCG 1 cut(s) 137
BsiHKCI CYCGRG 1 cut(s) 533
BsiSI CCGG 1 cut(s) 174
BslI CCNNNNNNNGG 2 cut(s) 322, 522
BsmAI GTCTC 1 cut(s) 4
BsnI GGCC 4 cut(s) 197, 304, 337, 701
Bso31I GGTCTC 1 cut(s) 4
BsoBI CYCGRG 1 cut(s) 533
Bsp119I TTCGAA 2 cut(s) 393, 763
Bsp143I GATC 3 cut(s) 134, 379, 631
Bsp19I CCATGG 1 cut(s) 442
BspACI CCGC 3 cut(s) 63, 517, 644
BspANI GGCC 4 cut(s) 197, 304, 337, 701
BspLI GGNNCC 2 cut(s) 232, 700
BspQI GCTCTTC 1 cut(s) 277
BspT104I TTCGAA 2 cut(s) 393, 763
BspTI CTTAAG 1 cut(s) 520
BspTNI GGTCTC 1 cut(s) 4
BsrI ACTGG 1 cut(s) 373
BssECI CCNNGG 3 cut(s) 299, 322, 442
BssMI GATC 3 cut(s) 134, 379, 631
BssNI GRCGYC 1 cut(s) 171
BssT1I CCWWGG 2 cut(s) 299, 442
Bst2UI CCWGG 1 cut(s) 323
Bst4CI ACNGT 1 cut(s) 242
Bst6I CTCTTC 3 cut(s) 99, 204, 277
BstACI GRCGYC 1 cut(s) 171
BstAFI CTTAAG 1 cut(s) 520
BstBI TTCGAA 2 cut(s) 393, 763
BstC8I GCNNGC 3 cut(s) 195, 492, 652
BstDSI CCRYGG 1 cut(s) 442
BstF5I GGATG 3 cut(s) 97, 481, 652
BstH2I RGCGCY 1 cut(s) 72
BstHHI GCGC 1 cut(s) 71
BstKTI GATC 3 cut(s) 137, 382, 634
BstMAI GTCTC 1 cut(s) 4
BstMBI GATC 3 cut(s) 134, 379, 631
BstMCI CGRYCG 1 cut(s) 137
BstMWI GCNNNNNNNGC 6 cut(s) 60, 155, 257, 293, 310, 656
BstNI CCWGG 1 cut(s) 323
BstNSI RCATGY 1 cut(s) 668
BstSCI CCNGG 1 cut(s) 321
BstV1I GCAGC 4 cut(s) 78, 104, 202, 610
BstV2I GAAGAC 1 cut(s) 752
BstXI CCANNNNNNTGG 1 cut(s) 329
BsuRI GGCC 4 cut(s) 197, 304, 337, 701
BtgI CCRYGG 1 cut(s) 442
BtrI CACGTC 1 cut(s) 400
BtsCI GGATG 3 cut(s) 97, 481, 652
BtsIMutI CAGTG 1 cut(s) 366
Cac8I GCNNGC 3 cut(s) 195, 492, 652
CfoI GCGC 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 699
CseI GACGC 2 cut(s) 164, 179
Csp6I GTAC 4 cut(s) 180, 409, 464, 662
CviAII CATG 4 cut(s) 429, 443, 641, 665
CviQI GTAC 4 cut(s) 180, 409, 464, 662
DpnI GATC 3 cut(s) 136, 381, 633
DpnII GATC 3 cut(s) 134, 379, 631
DraI TTTAAA 1 cut(s) 619
EaeI YGGCCR 1 cut(s) 195
Eam1104I CTCTTC 3 cut(s) 99, 204, 277
EarI CTCTTC 3 cut(s) 99, 204, 277
Eco130I CCWWGG 2 cut(s) 299, 442
Eco31I GGTCTC 1 cut(s) 4
Eco47III AGCGCT 1 cut(s) 70
Eco57I CTGAAG 1 cut(s) 564
Eco88I CYCGRG 1 cut(s) 533
EcoRI GAATTC 1 cut(s) 771
EcoRII CCWGG 1 cut(s) 321
EcoT14I CCWWGG 2 cut(s) 299, 442
ErhI CCWWGG 2 cut(s) 299, 442
FaeI CATG 4 cut(s) 432, 446, 644, 668
FatI CATG 4 cut(s) 428, 442, 640, 664
FauI CCCGC 1 cut(s) 524
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 5 cut(s) 64, 67, 118, 191, 624
FokI GGATG 3 cut(s) 84, 488, 659
Fsp4HI GCNGC 5 cut(s) 64, 67, 118, 191, 624
FspBI CTAG 2 cut(s) 350, 417
GlaI GCGC 1 cut(s) 70
GluI GCNGC 5 cut(s) 64, 67, 118, 191, 624
GsuI CTGGAG 2 cut(s) 240, 501
HaeII RGCGCY 1 cut(s) 72
HaeIII GGCC 4 cut(s) 197, 304, 337, 701
HapII CCGG 1 cut(s) 174
HgaI GACGC 2 cut(s) 164, 179
HhaI GCGC 1 cut(s) 71
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 4 cut(s) 432, 446, 644, 668
Hin6I GCGC 1 cut(s) 69
HinP1I GCGC 1 cut(s) 69
HindIII AAGCTT 1 cut(s) 488
HinfI GANTC 3 cut(s) 425, 512, 785
HpaII CCGG 1 cut(s) 174
HphI GGTGA 3 cut(s) 434, 683, 754
Hpy166II GTNNAC 2 cut(s) 217, 437
Hpy188I TCNGA 9 cut(s) 134, 281, 317, 361, 379, 583, 595, 757, 784
Hpy188III TCNNGA 1 cut(s) 383
Hpy8I GTNNAC 2 cut(s) 217, 437
Hpy99I CGWCG 2 cut(s) 158, 209
HpyAV CCTTC 2 cut(s) 26, 298
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4IV ACGT 1 cut(s) 399
HpyCH4V TGCA 5 cut(s) 190, 251, 553, 608, 686
HpyF10VI GCNNNNNNNGC 6 cut(s) 60, 155, 257, 293, 310, 656
HpySE526I ACGT 1 cut(s) 399
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 4 cut(s) 432, 446, 644, 668
HspAI GCGC 1 cut(s) 69
Kzo9I GATC 3 cut(s) 134, 379, 631
LguI GCTCTTC 1 cut(s) 277
LmnI GCTCC 5 cut(s) 5, 301, 318, 446, 538
Lsp1109I GCAGC 4 cut(s) 78, 104, 202, 610
LweI GCATC 5 cut(s) 466, 553, 562, 595, 637
MaeI CTAG 2 cut(s) 350, 417
MaeII ACGT 1 cut(s) 399
MaeIII GTNAC 1 cut(s) 244
MalI GATC 3 cut(s) 136, 381, 633
MboI GATC 3 cut(s) 134, 379, 631
MboII GAAGA 5 cut(s) 86, 221, 294, 570, 757
MlsI TGGCCA 1 cut(s) 197
MluCI AATT 3 cut(s) 505, 714, 771
MluNI TGGCCA 1 cut(s) 197
MlyI GAGTC 1 cut(s) 506
MmeI TCCRAC 3 cut(s) 147, 691, 780
Mox20I TGGCCA 1 cut(s) 197
MroXI GAANNNNTTC 1 cut(s) 749
MscI TGGCCA 1 cut(s) 197
MseI TTAA 4 cut(s) 87, 486, 521, 618
MslI CAYNNNNRTG 2 cut(s) 645, 669
Msp20I TGGCCA 1 cut(s) 197
MspA1I CMGCKG 2 cut(s) 193, 260
MspCI CTTAAG 1 cut(s) 520
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 1 cut(s) 323
MvaI CCWGG 1 cut(s) 323
MwoI GCNNNNNNNGC 6 cut(s) 60, 155, 257, 293, 310, 656
NcoI CCATGG 1 cut(s) 442
NdeII GATC 3 cut(s) 134, 379, 631
NlaIII CATG 4 cut(s) 432, 446, 644, 668
NlaIV GGNNCC 2 cut(s) 232, 700
NmuCI GTSAC 1 cut(s) 244
NspI RCATGY 1 cut(s) 668
NspV TTCGAA 2 cut(s) 393, 763
PaeR7I CTCGAG 1 cut(s) 533
PciI ACATGT 1 cut(s) 664
PciSI GCTCTTC 1 cut(s) 277
PdmI GAANNNNTTC 1 cut(s) 749
PfeI GAWTC 1 cut(s) 425
PkrI GCNGC 5 cut(s) 65, 68, 119, 192, 625
Ple19I CGATCG 1 cut(s) 137
PleI GAGTC 1 cut(s) 506
PpsI GAGTC 1 cut(s) 506
PscI ACATGT 1 cut(s) 664
Psp6I CCWGG 1 cut(s) 321
PspGI CCWGG 1 cut(s) 321
PspN4I GGNNCC 2 cut(s) 232, 700
PspPI GGNCC 1 cut(s) 699
PspXI VCTCGAGB 1 cut(s) 533
PvuI CGATCG 1 cut(s) 137
PvuII CAGCTG 2 cut(s) 193, 260
RsaI GTAC 4 cut(s) 181, 410, 465, 663
RsaNI GTAC 4 cut(s) 180, 409, 464, 662
RseI CAYNNNNRTG 2 cut(s) 645, 669
SapI GCTCTTC 1 cut(s) 277
SaqAI TTAA 4 cut(s) 87, 486, 521, 618
SatI GCNGC 5 cut(s) 64, 67, 118, 191, 624
Sau3AI GATC 3 cut(s) 134, 379, 631
Sau96I GGNCC 1 cut(s) 699
ScaI AGTACT 1 cut(s) 410
SchI GAGTC 1 cut(s) 506
ScrFI CCNGG 1 cut(s) 323
SfaNI GCATC 5 cut(s) 466, 553, 562, 595, 637
Sfr274I CTCGAG 1 cut(s) 533
SfuI TTCGAA 2 cut(s) 393, 763
SlaI CTCGAG 1 cut(s) 533
SmiMI CAYNNNNRTG 2 cut(s) 645, 669
SmlI CTYRAG 3 cut(s) 470, 520, 533
SmoI CTYRAG 3 cut(s) 470, 520, 533
SpeI ACTAGT 1 cut(s) 349
Sse9I AATT 3 cut(s) 505, 714, 771
SsiI CCGC 3 cut(s) 63, 517, 644
SspMI CTAG 2 cut(s) 350, 417
StyD4I CCNGG 1 cut(s) 321
StyI CCWWGG 2 cut(s) 299, 442
TaaI ACNGT 1 cut(s) 242
TaiI ACGT 1 cut(s) 402
TaqI TCGA 5 cut(s) 207, 393, 503, 534, 763
TasI AATT 3 cut(s) 505, 714, 771
TatI WGTACW 2 cut(s) 408, 661
TauI GCSGC 1 cut(s) 66
TfiI GAWTC 1 cut(s) 425
Tru1I TTAA 4 cut(s) 87, 486, 521, 618
Tru9I TTAA 4 cut(s) 87, 486, 521, 618
TscAI CASTG 1 cut(s) 373
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 4 cut(s) 66, 117, 190, 623
Tsp45I GTSAC 1 cut(s) 244
TspDTI ATGAA 4 cut(s) 86, 417, 468, 693
TspRI CASTG 1 cut(s) 373
Vha464I CTTAAG 1 cut(s) 520
XapI RAATTY 2 cut(s) 505, 771
XceI RCATGY 1 cut(s) 668
XhoI CTCGAG 1 cut(s) 533
XmiI GTMKAC 1 cut(s) 216
XmnI GAANNNNTTC 1 cut(s) 749
XspI CTAG 2 cut(s) 350, 417
ZrmI AGTACT 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.