Rh4AG374000

Haloacid dehalogenase-like hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0007289
Physical Location & Seq
Forward (+)
68147596 .. 68148727
1132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG374000.1

Sequence Viewer

Length: 663 bp
ATGGGAGCGAGACCCCTTCGCTTTGGTAAAATGGAGGGTTGCTTGGTCAAATGCTCTCACGGCGGCAGCGCTCTTTTGAAAACCCTTAAACCTCTTCATCCCAAAACCCACATTCGCTGCTTCTCTGCCCTCCGATCGCCTCGCAGAGCCTACGACGCATTGTTGTTGGACGCCGGAGGTACTCTCTTGCAGCTGGCCAACCCCGTCGAAGAGGTCTACGCCTCCATAGGAACCCAATACGGTGTGACTGCAACTCCAGCTGAAATCAAGCAGGGTTTCAGAAGAGCCTTCTCTGCTCCTTGGCCTCAAAAGCTCCGATACCAGGGAGATGGGAGGCCATTTTGGAAACTAGTTGTTTCAGAAGCCACTGGTTGTTCTGATCTTGATTATTTCGAACACGTCTATCAGTACTATGCTAGAGGTGATTCATGGCGTTTACCCCATGGAGCTTATGAAACAATGGTACTTCTCAAGGATGCTGGAGTTAAGCTTGCTGTTGTATCGAATTTTGACTCCCGCTTAAGGAAACTACTCGAGGAGCTGAATGTGTTGCATCTGTTTGATGCTGTTATCATATCTTCAGAGGTTGGTTCTGAAAAACCAGATGCAAACATATTTAAAGCTGCGTTAGGTATGTATTGCAGCACCATGTTCAGCGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2

Protein Analysis

220

Amino Acids

24.39

Weight (kDa)

9.05

Isoelectric Point (pI)

41.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 51 - 210 4.7e-14 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 130 - 210 3.6e-08 Haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 216
AciI CCGC 3 cut(s) 63, 517, 657
AcoI YGGCCR 1 cut(s) 195
AcsI RAATTY 1 cut(s) 505
AcuI CTGAAG 1 cut(s) 564
AcyI GRCGYC 1 cut(s) 171
AfaI GTAC 3 cut(s) 181, 410, 465
AfeI AGCGCT 1 cut(s) 70
AfiI CCNNNNNNNGG 2 cut(s) 322, 522
AflII CTTAAG 1 cut(s) 520
AflIII ACRYGT 1 cut(s) 397
AgsI TTSAA 1 cut(s) 79
AhlI ACTAGT 1 cut(s) 349
AjiI CACGTC 1 cut(s) 400
AjnI CCWGG 1 cut(s) 321
AluBI AGCT 7 cut(s) 193, 260, 313, 449, 490, 541, 623
AluI AGCT 7 cut(s) 193, 260, 313, 449, 490, 541, 623
Alw26I GTCTC 1 cut(s) 4
Ama87I CYCGRG 1 cut(s) 533
Aor51HI AGCGCT 1 cut(s) 70
AoxI GGCC 3 cut(s) 195, 302, 335
ApeKI GCWGC 5 cut(s) 66, 117, 190, 623, 642
ApoI RAATTY 1 cut(s) 505
AspLEI GCGC 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 434
AsuII TTCGAA 1 cut(s) 393
AvaI CYCGRG 1 cut(s) 533
BalI TGGCCA 1 cut(s) 197
BbvI GCAGC 5 cut(s) 78, 104, 202, 610, 654
BccI CCATC 1 cut(s) 323
BceAI ACGGC 1 cut(s) 76
BciT130I CCWGG 1 cut(s) 323
BcoDI GTCTC 1 cut(s) 4
BcuI ACTAGT 1 cut(s) 349
BfaI CTAG 2 cut(s) 350, 417
BfoI RGCGCY 1 cut(s) 72
BfrI CTTAAG 1 cut(s) 520
BisI GCNGC 6 cut(s) 64, 67, 118, 191, 624, 643
BlsI GCNGC 6 cut(s) 65, 68, 119, 192, 625, 644
BmcAI AGTACT 1 cut(s) 410
Bme1390I CCNGG 1 cut(s) 323
BmeT110I CYCGRG 1 cut(s) 533
BmgBI CACGTC 1 cut(s) 400
BmiI GGNNCC 1 cut(s) 232
BmrFI CCNGG 1 cut(s) 323
BmsI GCATC 4 cut(s) 466, 553, 562, 595
BplI GAGNNNNNCTC 2 cut(s) 168, 200
BpmI CTGGAG 2 cut(s) 240, 501
Bpu14I TTCGAA 1 cut(s) 393
BpuEI CTTGAG 1 cut(s) 455
BsaHI GRCGYC 1 cut(s) 171
BsaI GGTCTC 1 cut(s) 4
BsaJI CCNNGG 3 cut(s) 299, 322, 442
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bsc4I CCNNNNNNNGG 2 cut(s) 322, 522
Bse1I ACTGG 1 cut(s) 373
BseBI CCWGG 1 cut(s) 323
BseDI CCNNGG 3 cut(s) 299, 322, 442
BseGI GGATG 2 cut(s) 97, 481
BseLI CCNNNNNNNGG 2 cut(s) 322, 522
BseNI ACTGG 1 cut(s) 373
BseRI GAGGAG 1 cut(s) 551
BseXI GCAGC 5 cut(s) 78, 104, 202, 610, 654
Bsh1285I CGRYCG 1 cut(s) 137
BshFI GGCC 3 cut(s) 197, 304, 337
BsiEI CGRYCG 1 cut(s) 137
BsiHKCI CYCGRG 1 cut(s) 533
BsiSI CCGG 1 cut(s) 174
BslI CCNNNNNNNGG 2 cut(s) 322, 522
BsmAI GTCTC 1 cut(s) 4
BsnI GGCC 3 cut(s) 197, 304, 337
Bso31I GGTCTC 1 cut(s) 4
BsoBI CYCGRG 1 cut(s) 533
Bsp119I TTCGAA 1 cut(s) 393
Bsp143I GATC 2 cut(s) 134, 379
Bsp19I CCATGG 1 cut(s) 442
BspACI CCGC 3 cut(s) 63, 517, 657
BspANI GGCC 3 cut(s) 197, 304, 337
BspLI GGNNCC 1 cut(s) 232
BspQI GCTCTTC 1 cut(s) 277
BspT104I TTCGAA 1 cut(s) 393
BspTI CTTAAG 1 cut(s) 520
BspTNI GGTCTC 1 cut(s) 4
BsrI ACTGG 1 cut(s) 373
BssECI CCNNGG 3 cut(s) 299, 322, 442
BssMI GATC 2 cut(s) 134, 379
BssNI GRCGYC 1 cut(s) 171
BssT1I CCWWGG 2 cut(s) 299, 442
Bst2UI CCWGG 1 cut(s) 323
Bst4CI ACNGT 1 cut(s) 242
Bst6I CTCTTC 3 cut(s) 99, 204, 277
BstACI GRCGYC 1 cut(s) 171
BstAFI CTTAAG 1 cut(s) 520
BstBI TTCGAA 1 cut(s) 393
BstC8I GCNNGC 2 cut(s) 195, 492
BstDSI CCRYGG 1 cut(s) 442
BstF5I GGATG 2 cut(s) 97, 481
BstH2I RGCGCY 1 cut(s) 72
BstHHI GCGC 1 cut(s) 71
BstKTI GATC 2 cut(s) 137, 382
BstMAI GTCTC 1 cut(s) 4
BstMBI GATC 2 cut(s) 134, 379
BstMCI CGRYCG 1 cut(s) 137
BstMWI GCNNNNNNNGC 5 cut(s) 60, 155, 257, 293, 310
BstNI CCWGG 1 cut(s) 323
BstSCI CCNGG 1 cut(s) 321
BstV1I GCAGC 5 cut(s) 78, 104, 202, 610, 654
BstXI CCANNNNNNTGG 1 cut(s) 329
BsuRI GGCC 3 cut(s) 197, 304, 337
BtgI CCRYGG 1 cut(s) 442
BtrI CACGTC 1 cut(s) 400
BtsCI GGATG 2 cut(s) 97, 481
BtsIMutI CAGTG 1 cut(s) 366
Cac8I GCNNGC 2 cut(s) 195, 492
CfoI GCGC 1 cut(s) 71
CseI GACGC 2 cut(s) 164, 179
Csp6I GTAC 3 cut(s) 180, 409, 464
CviAII CATG 3 cut(s) 429, 443, 649
CviQI GTAC 3 cut(s) 180, 409, 464
DpnI GATC 2 cut(s) 136, 381
DpnII GATC 2 cut(s) 134, 379
DraI TTTAAA 1 cut(s) 619
EaeI YGGCCR 1 cut(s) 195
Eam1104I CTCTTC 3 cut(s) 99, 204, 277
EarI CTCTTC 3 cut(s) 99, 204, 277
Eco130I CCWWGG 2 cut(s) 299, 442
Eco31I GGTCTC 1 cut(s) 4
Eco47III AGCGCT 1 cut(s) 70
Eco57I CTGAAG 1 cut(s) 564
Eco88I CYCGRG 1 cut(s) 533
EcoRII CCWGG 1 cut(s) 321
EcoT14I CCWWGG 2 cut(s) 299, 442
ErhI CCWWGG 2 cut(s) 299, 442
FaeI CATG 3 cut(s) 432, 446, 652
FaiI YATR 9 cut(s) 227, 414, 430, 444, 453, 575, 614, 635, 650
FatI CATG 3 cut(s) 428, 442, 648
FauI CCCGC 1 cut(s) 524
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 6 cut(s) 64, 67, 118, 191, 624, 643
FokI GGATG 2 cut(s) 84, 488
Fsp4HI GCNGC 6 cut(s) 64, 67, 118, 191, 624, 643
FspBI CTAG 2 cut(s) 350, 417
GlaI GCGC 1 cut(s) 70
GluI GCNGC 6 cut(s) 64, 67, 118, 191, 624, 643
GsuI CTGGAG 2 cut(s) 240, 501
HaeII RGCGCY 1 cut(s) 72
HaeIII GGCC 3 cut(s) 197, 304, 337
HapII CCGG 1 cut(s) 174
HgaI GACGC 2 cut(s) 164, 179
HhaI GCGC 1 cut(s) 71
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 3 cut(s) 432, 446, 652
Hin6I GCGC 1 cut(s) 69
HinP1I GCGC 1 cut(s) 69
HindIII AAGCTT 1 cut(s) 488
HinfI GANTC 2 cut(s) 425, 512
HpaII CCGG 1 cut(s) 174
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 2 cut(s) 217, 437
Hpy188I TCNGA 7 cut(s) 134, 281, 317, 361, 379, 583, 595
Hpy188III TCNNGA 1 cut(s) 383
Hpy8I GTNNAC 2 cut(s) 217, 437
Hpy99I CGWCG 2 cut(s) 158, 209
HpyAV CCTTC 2 cut(s) 26, 298
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4IV ACGT 1 cut(s) 399
HpyCH4V TGCA 5 cut(s) 190, 251, 553, 608, 642
HpyF10VI GCNNNNNNNGC 5 cut(s) 60, 155, 257, 293, 310
HpySE526I ACGT 1 cut(s) 399
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 3 cut(s) 432, 446, 652
HspAI GCGC 1 cut(s) 69
Kzo9I GATC 2 cut(s) 134, 379
LguI GCTCTTC 1 cut(s) 277
LmnI GCTCC 5 cut(s) 5, 301, 318, 446, 538
LpnPI CCDG 9 cut(s) 179, 187, 257, 270, 308, 335, 354, 465, 615
Lsp1109I GCAGC 5 cut(s) 78, 104, 202, 610, 654
LweI GCATC 4 cut(s) 466, 553, 562, 595
MaeI CTAG 2 cut(s) 350, 417
MaeII ACGT 1 cut(s) 399
MaeIII GTNAC 1 cut(s) 244
MalI GATC 2 cut(s) 136, 381
MboI GATC 2 cut(s) 134, 379
MboII GAAGA 4 cut(s) 86, 221, 294, 570
MlsI TGGCCA 1 cut(s) 197
MluCI AATT 1 cut(s) 505
MluNI TGGCCA 1 cut(s) 197
MlyI GAGTC 1 cut(s) 506
MmeI TCCRAC 1 cut(s) 147
Mox20I TGGCCA 1 cut(s) 197
MscI TGGCCA 1 cut(s) 197
MseI TTAA 4 cut(s) 87, 486, 521, 618
Msp20I TGGCCA 1 cut(s) 197
MspA1I CMGCKG 3 cut(s) 193, 260, 657
MspCI CTTAAG 1 cut(s) 520
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 1 cut(s) 323
MvaI CCWGG 1 cut(s) 323
MwoI GCNNNNNNNGC 5 cut(s) 60, 155, 257, 293, 310
NcoI CCATGG 1 cut(s) 442
NdeII GATC 2 cut(s) 134, 379
NlaIII CATG 3 cut(s) 432, 446, 652
NlaIV GGNNCC 1 cut(s) 232
NmuCI GTSAC 1 cut(s) 244
NspV TTCGAA 1 cut(s) 393
PaeR7I CTCGAG 1 cut(s) 533
PciSI GCTCTTC 1 cut(s) 277
PfeI GAWTC 1 cut(s) 425
PkrI GCNGC 6 cut(s) 65, 68, 119, 192, 625, 644
Ple19I CGATCG 1 cut(s) 137
PleI GAGTC 1 cut(s) 506
PpsI GAGTC 1 cut(s) 506
Psp6I CCWGG 1 cut(s) 321
PspGI CCWGG 1 cut(s) 321
PspN4I GGNNCC 1 cut(s) 232
PspXI VCTCGAGB 1 cut(s) 533
PvuI CGATCG 1 cut(s) 137
PvuII CAGCTG 2 cut(s) 193, 260
RsaI GTAC 3 cut(s) 181, 410, 465
RsaNI GTAC 3 cut(s) 180, 409, 464
SapI GCTCTTC 1 cut(s) 277
SaqAI TTAA 4 cut(s) 87, 486, 521, 618
SatI GCNGC 6 cut(s) 64, 67, 118, 191, 624, 643
Sau3AI GATC 2 cut(s) 134, 379
ScaI AGTACT 1 cut(s) 410
SchI GAGTC 1 cut(s) 506
ScrFI CCNGG 1 cut(s) 323
SfaNI GCATC 4 cut(s) 466, 553, 562, 595
Sfr274I CTCGAG 1 cut(s) 533
SfuI TTCGAA 1 cut(s) 393
SlaI CTCGAG 1 cut(s) 533
SmlI CTYRAG 3 cut(s) 470, 520, 533
SmoI CTYRAG 3 cut(s) 470, 520, 533
SpeI ACTAGT 1 cut(s) 349
Sse9I AATT 1 cut(s) 505
SsiI CCGC 3 cut(s) 63, 517, 657
SspMI CTAG 2 cut(s) 350, 417
StyD4I CCNGG 1 cut(s) 321
StyI CCWWGG 2 cut(s) 299, 442
TaaI ACNGT 1 cut(s) 242
TaiI ACGT 1 cut(s) 402
TaqI TCGA 4 cut(s) 207, 393, 503, 534
TasI AATT 1 cut(s) 505
TatI WGTACW 1 cut(s) 408
TauI GCSGC 1 cut(s) 66
TfiI GAWTC 1 cut(s) 425
Tru1I TTAA 4 cut(s) 87, 486, 521, 618
Tru9I TTAA 4 cut(s) 87, 486, 521, 618
TscAI CASTG 1 cut(s) 373
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 5 cut(s) 66, 117, 190, 623, 642
Tsp45I GTSAC 1 cut(s) 244
TspDTI ATGAA 3 cut(s) 86, 417, 468
TspRI CASTG 1 cut(s) 373
Vha464I CTTAAG 1 cut(s) 520
XapI RAATTY 1 cut(s) 505
XhoI CTCGAG 1 cut(s) 533
XmiI GTMKAC 1 cut(s) 216
XspI CTAG 2 cut(s) 350, 417
ZrmI AGTACT 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.