Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007289

Physical Location & Seq

Genomic Coordinates Forward (+)

5199882 .. 5201773

Gene Structure 1892 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1074400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 792 bp

ATGGAAGGTTGCTTGATGAGATGGTCTCACGGCGGCGCTCTCCTGAAAGCCCTCAAACCTCTCCATGTGAACGTCCCTAAACACTCCATTCGCTGCTCTTCCATGTCCGTCCACTCCGGCGGGGGAGGATCGGCTGTCAGCCGCAGGGCCTACGACGCACTGCTGTTAGACGCCGGAGGCACTCTGCTTCAGCTGAAAAAACCAGTCGAAGAAGTTTACGCTTCCATCGGAACCAAATACGGTGTGACTGCAACTCCAGCTGAAATCAAGCAAGGTTTCAGAAGAGCTTTTTCTGCTACTTGGCCTCAGAAGCTTCGTTACCAGGGAGATGGGAGGCCGTTTTGGAAACTTGTTGTGTCTGAAGCCACTGGTTGTGCTAATCTCGATTACTTCGAACAAGTTTATGAGTACTATGCGAAAGGTGACTCATGGCGTCTTCCGGATGGAGCTTATGAAACAATGCTCCTTCTCAAAGATGCTGGAGTTAAGCTGGCTGTTGTATCGAATTTCGACTCCCGCTTGAGGAAGCTGCTTGAGGACCTTAATGTTTTGCATCTGTTTGATGCTGTTATCATATCTTCAGAAGTTGGGTATGAAAAACCAGATGCAGGCATATTCAAAGCTGCTTTAGATCAAGTTCATGTGGATGCTGGCAAAGCAGTACATGTTGGTGACGATAAAATTGCGGATAAGGATGGCGCCAATGCCATTGGAATCGATTGCTTGTTATGGGGTCTAGATGTGAAGACATTCTCTGAAATTCGAAACAGCATTCTCATTTCAGAGTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAD_2

Protein Analysis

264

Amino Acids

28.72

Weight (kDa)

6.66

Isoelectric Point (pI)

33.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase	PF00702	51 - 238	2.6e-20	haloacid dehalogenase-like hydrolase
HAD_2	PF13419	128 - 242	3.1e-15	Haloacid dehalogenase-like hydrolase
Hydrolase_like	PF13242	199 - 243	2.3e-06	HAD-hyrolase-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007289)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14310
fragaria_vesca	FvH4_4g31490 FvH4_4g31490 FvH4_4g31490 FvH4_4g31490 FvH4_4g31490
malus_domestica	MD05G1042500.v1.1 MD13G1073200.v1.1 MD16G1074400.v1.1
prunus_persica	Prupe.1G272900_v2.0.a1
pyrus_communis	pycom13g06560 pycom16g06440 pycom17g12220
rosa_chinensis	RchiOBHm_Chr4g0439981
rosa_laevigata	RLG00000006218
rosa_multiflora	Rmu_sc0014336.1_g000001
rosa_roxburghii	Rroxscaffold_5G00380930
rosa_rugosa	Rorug04G0322800 Rorug04G0322900
rosa_samantha	Rh1BG026500 Rh4AG374000 Rh4BG386300 Rh4CG400800 Rh4DG380600
rosa_wichuraiana	Rw4G031990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	698
AccIII	TCCGGA	1 cut(s)	439
AciI	CCGC	5 cut(s)	33, 120, 142, 517, 686
AclWI	GGATC	1 cut(s)	136
AcsI	RAATTY	2 cut(s)	505, 759
AcuI	CTGAAG	3 cut(s)	173, 381, 564
AcyI	GRCGYC	3 cut(s)	171, 433, 699
AfaI	GTAC	2 cut(s)	410, 663
AfiI	CCNNNNNNNGG	2 cut(s)	522, 608
AflIII	ACRYGT	1 cut(s)	664
AgsI	TTSAA	1 cut(s)	619
AjnI	CCWGG	1 cut(s)	321
AluBI	AGCT	8 cut(s)	193, 260, 287, 313, 449, 490, 529, 623
AluI	AGCT	8 cut(s)	193, 260, 287, 313, 449, 490, 529, 623
Alw26I	GTCTC	1 cut(s)	30
AlwI	GGATC	1 cut(s)	136
Aor13HI	TCCGGA	1 cut(s)	439
AoxI	GGCC	3 cut(s)	147, 302, 335
ApeKI	GCWGC	3 cut(s)	93, 529, 623
ApoI	RAATTY	2 cut(s)	505, 759
Asp700I	GAANNNNTTC	1 cut(s)	749
AspLEI	GCGC	2 cut(s)	38, 701
AspS9I	GGNCC	2 cut(s)	147, 538
AsuHPI	GGTGA	2 cut(s)	434, 683
AsuII	TTCGAA	2 cut(s)	393, 763
AvaII	GGWCC	1 cut(s)	538
BanI	GGYRCC	1 cut(s)	698
BarI	GAAGNNNNNNTAC	2 cut(s)	302, 334
BbsI	GAAGAC	2 cut(s)	428, 752
BbvI	GCAGC	3 cut(s)	80, 516, 610
BccI	CCATC	5 cut(s)	15, 233, 323, 437, 689
BceAI	ACGGC	2 cut(s)	46, 322
BcgI	CGANNNNNNTGC	2 cut(s)	665, 699
BciT130I	CCWGG	1 cut(s)	323
BcoDI	GTCTC	1 cut(s)	30
BfaI	CTAG	1 cut(s)	737
BfoI	RGCGCY	2 cut(s)	39, 702
BisI	GCNGC	5 cut(s)	34, 94, 142, 530, 624
BlsI	GCNGC	5 cut(s)	35, 95, 143, 531, 625
BmcAI	AGTACT	1 cut(s)	410
Bme1390I	CCNGG	1 cut(s)	323
Bme18I	GGWCC	1 cut(s)	538
BmgT120I	GGNCC	2 cut(s)	147, 538
BmiI	GGNNCC	2 cut(s)	232, 700
BmrFI	CCNGG	1 cut(s)	323
BmsI	GCATC	5 cut(s)	466, 553, 562, 595, 637
BpiI	GAAGAC	2 cut(s)	428, 752
BplI	GAGNNNNNCTC	2 cut(s)	10, 42
BpmI	CTGGAG	2 cut(s)	240, 501
Bpu14I	TTCGAA	2 cut(s)	393, 763
BpuEI	CTTGAG	2 cut(s)	541, 554
Bsa29I	ATCGAT	1 cut(s)	717
BsaHI	GRCGYC	3 cut(s)	171, 433, 699
BsaI	GGTCTC	1 cut(s)	30
BsaJI	CCNNGG	1 cut(s)	322
BsaWI	WCCGGW	1 cut(s)	439
BsaXI	ACNNNNNCTCC	2 cut(s)	238, 268
Bsc4I	CCNNNNNNNGG	2 cut(s)	522, 608
Bse1I	ACTGG	2 cut(s)	203, 373
BseAI	TCCGGA	1 cut(s)	439
BseBI	CCWGG	1 cut(s)	323
BseCI	ATCGAT	1 cut(s)	717
BseDI	CCNNGG	1 cut(s)	322
BseGI	GGATG	3 cut(s)	448, 652, 700
BseLI	CCNNNNNNNGG	2 cut(s)	522, 608
BseMII	CTCAG	1 cut(s)	320
BseNI	ACTGG	2 cut(s)	203, 373
BseXI	GCAGC	3 cut(s)	80, 516, 610
BshFI	GGCC	3 cut(s)	149, 304, 337
BshNI	GGYRCC	1 cut(s)	698
BshVI	ATCGAT	1 cut(s)	717
BsiSI	CCGG	3 cut(s)	117, 174, 440
BslFI	GGGAC	1 cut(s)	59
BslI	CCNNNNNNNGG	2 cut(s)	522, 608
BsmAI	GTCTC	1 cut(s)	30
BsmFI	GGGAC	1 cut(s)	59
BsmI	GAATGC	1 cut(s)	771
BsnI	GGCC	3 cut(s)	149, 304, 337
Bso31I	GGTCTC	1 cut(s)	30
Bsp119I	TTCGAA	2 cut(s)	393, 763
Bsp13I	TCCGGA	1 cut(s)	439
Bsp143I	GATC	2 cut(s)	128, 631
BspACI	CCGC	5 cut(s)	33, 120, 142, 517, 686
BspANI	GGCC	3 cut(s)	149, 304, 337
BspCNI	CTCAG	1 cut(s)	319
BspDI	ATCGAT	1 cut(s)	717
BspEI	TCCGGA	1 cut(s)	439
BspLI	GGNNCC	2 cut(s)	232, 700
BspPI	GGATC	1 cut(s)	136
BspQI	GCTCTTC	2 cut(s)	103, 277
BspT104I	TTCGAA	2 cut(s)	393, 763
BspT107I	GGYRCC	1 cut(s)	698
BspTNI	GGTCTC	1 cut(s)	30
BsrI	ACTGG	2 cut(s)	203, 373
BssECI	CCNNGG	1 cut(s)	322
BssMI	GATC	2 cut(s)	128, 631
BssNI	GRCGYC	3 cut(s)	171, 433, 699
Bst2UI	CCWGG	1 cut(s)	323
Bst4CI	ACNGT	1 cut(s)	242
Bst6I	CTCTTC	2 cut(s)	103, 277
BstACI	GRCGYC	3 cut(s)	171, 433, 699
BstBI	TTCGAA	2 cut(s)	393, 763
BstC8I	GCNNGC	3 cut(s)	492, 610, 652
BstDEI	CTNAG	1 cut(s)	306
BstF5I	GGATG	3 cut(s)	448, 652, 700
BstH2I	RGCGCY	2 cut(s)	39, 702
BstHHI	GCGC	2 cut(s)	38, 701
BstKTI	GATC	2 cut(s)	131, 634
BstMAI	GTCTC	1 cut(s)	30
BstMBI	GATC	2 cut(s)	128, 631
BstMWI	GCNNNNNNNGC	5 cut(s)	155, 257, 293, 310, 656
BstNI	CCWGG	1 cut(s)	323
BstNSI	RCATGY	1 cut(s)	668
BstSCI	CCNGG	1 cut(s)	321
BstV1I	GCAGC	3 cut(s)	80, 516, 610
BstV2I	GAAGAC	2 cut(s)	428, 752
BstXI	CCANNNNNNTGG	1 cut(s)	329
Bsu15I	ATCGAT	1 cut(s)	717
BsuRI	GGCC	3 cut(s)	149, 304, 337
BsuTUI	ATCGAT	1 cut(s)	717
BtsCI	GGATG	3 cut(s)	448, 652, 700
BtsI	GCAGTG	1 cut(s)	158
BtsIMutI	CAGTG	2 cut(s)	158, 366
Cac8I	GCNNGC	3 cut(s)	492, 610, 652
CfoI	GCGC	2 cut(s)	38, 701
Cfr13I	GGNCC	2 cut(s)	147, 538
ClaI	ATCGAT	1 cut(s)	717
CseI	GACGC	3 cut(s)	164, 179, 422
Csp6I	GTAC	2 cut(s)	409, 662
CviAII	CATG	5 cut(s)	65, 103, 429, 641, 665
CviQI	GTAC	2 cut(s)	409, 662
DdeI	CTNAG	1 cut(s)	306
DinI	GGCGCC	1 cut(s)	700
DpnI	GATC	2 cut(s)	130, 633
DpnII	GATC	2 cut(s)	128, 631
Eam1104I	CTCTTC	2 cut(s)	103, 277
EarI	CTCTTC	2 cut(s)	103, 277
Eco31I	GGTCTC	1 cut(s)	30
Eco47I	GGWCC	1 cut(s)	538
Eco57I	CTGAAG	3 cut(s)	173, 381, 564
EcoO109I	RGGNCCY	2 cut(s)	147, 538
EcoRII	CCWGG	1 cut(s)	321
EgeI	GGCGCC	1 cut(s)	700
EheI	GGCGCC	1 cut(s)	700
FaeI	CATG	5 cut(s)	68, 106, 432, 644, 668
FaqI	GGGAC	1 cut(s)	59
FatI	CATG	5 cut(s)	64, 102, 428, 640, 664
FauI	CCCGC	2 cut(s)	113, 524
Fnu4HI	GCNGC	5 cut(s)	34, 94, 142, 530, 624
FokI	GGATG	3 cut(s)	455, 659, 707
Fsp4HI	GCNGC	5 cut(s)	34, 94, 142, 530, 624
FspBI	CTAG	1 cut(s)	737
GlaI	GCGC	2 cut(s)	37, 700
GluI	GCNGC	5 cut(s)	34, 94, 142, 530, 624
GsuI	CTGGAG	2 cut(s)	240, 501
HaeII	RGCGCY	2 cut(s)	39, 702
HaeIII	GGCC	3 cut(s)	149, 304, 337
HapII	CCGG	3 cut(s)	117, 174, 440
HgaI	GACGC	3 cut(s)	164, 179, 422
HhaI	GCGC	2 cut(s)	38, 701
Hin1I	GRCGYC	3 cut(s)	171, 433, 699
Hin1II	CATG	5 cut(s)	68, 106, 432, 644, 668
Hin6I	GCGC	2 cut(s)	36, 699
HinP1I	GCGC	2 cut(s)	36, 699
HindIII	AAGCTT	1 cut(s)	311
HinfI	GANTC	4 cut(s)	425, 512, 714, 785
HpaII	CCGG	3 cut(s)	117, 174, 440
HphI	GGTGA	2 cut(s)	434, 683
Hpy166II	GTNNAC	3 cut(s)	70, 112, 217
Hpy188I	TCNGA	7 cut(s)	230, 281, 309, 361, 583, 757, 784
Hpy188III	TCNNGA	4 cut(s)	43, 383, 440, 737
Hpy8I	GTNNAC	3 cut(s)	70, 112, 217
Hpy99I	CGWCG	1 cut(s)	158
HpyAV	CCTTC	1 cut(s)	476
HpyCH4III	ACNGT	1 cut(s)	242
HpyCH4IV	ACGT	1 cut(s)	72
HpyCH4V	TGCA	3 cut(s)	251, 553, 608
HpyF10VI	GCNNNNNNNGC	5 cut(s)	155, 257, 293, 310, 656
HpyF3I	CTNAG	1 cut(s)	306
HpySE526I	ACGT	1 cut(s)	72
Hsp92I	GRCGYC	3 cut(s)	171, 433, 699
Hsp92II	CATG	5 cut(s)	68, 106, 432, 644, 668
HspAI	GCGC	2 cut(s)	36, 699
KasI	GGCGCC	1 cut(s)	698
Kpn2I	TCCGGA	1 cut(s)	439
Kzo9I	GATC	2 cut(s)	128, 631
LguI	GCTCTTC	2 cut(s)	103, 277
LmnI	GCTCC	2 cut(s)	446, 468
Lsp1109I	GCAGC	3 cut(s)	80, 516, 610
LweI	GCATC	5 cut(s)	466, 553, 562, 595, 637
MaeI	CTAG	1 cut(s)	737
MaeII	ACGT	1 cut(s)	72
MaeIII	GTNAC	4 cut(s)	244, 317, 422, 671
MalI	GATC	2 cut(s)	130, 633
MboI	GATC	2 cut(s)	128, 631
MboII	GAAGA	6 cut(s)	90, 221, 294, 428, 570, 757
MluCI	AATT	3 cut(s)	505, 681, 759
Mly113I	GGCGCC	1 cut(s)	699
MlyI	GAGTC	2 cut(s)	419, 506
MnlI	CCTC	8 cut(s)	62, 69, 119, 170, 315, 327, 516, 529
MroI	TCCGGA	1 cut(s)	439
MroXI	GAANNNNTTC	1 cut(s)	749
MseI	TTAA	2 cut(s)	486, 543
MslI	CAYNNNNRTG	2 cut(s)	645, 669
MspA1I	CMGCKG	2 cut(s)	193, 260
MspI	CCGG	3 cut(s)	117, 174, 440
MspR9I	CCNGG	1 cut(s)	323
Mva1269I	GAATGC	1 cut(s)	771
MvaI	CCWGG	1 cut(s)	323
MwoI	GCNNNNNNNGC	5 cut(s)	155, 257, 293, 310, 656
NarI	GGCGCC	1 cut(s)	699
NdeII	GATC	2 cut(s)	128, 631
NlaIII	CATG	5 cut(s)	68, 106, 432, 644, 668
NlaIV	GGNNCC	2 cut(s)	232, 700
NmuCI	GTSAC	3 cut(s)	244, 422, 671
NspI	RCATGY	1 cut(s)	668
NspV	TTCGAA	2 cut(s)	393, 763
PciI	ACATGT	1 cut(s)	664
PciSI	GCTCTTC	2 cut(s)	103, 277
PcsI	WCGNNNNNNNCGW	1 cut(s)	390
PctI	GAATGC	1 cut(s)	771
PdmI	GAANNNNTTC	1 cut(s)	749
PfeI	GAWTC	1 cut(s)	714
PkrI	GCNGC	5 cut(s)	35, 95, 143, 531, 625
PleI	GAGTC	2 cut(s)	419, 506
PluTI	GGCGCC	1 cut(s)	702
PpsI	GAGTC	2 cut(s)	419, 506
PpuMI	RGGWCCY	1 cut(s)	538
PscI	ACATGT	1 cut(s)	664
Psp5II	RGGWCCY	1 cut(s)	538
Psp6I	CCWGG	1 cut(s)	321
PspGI	CCWGG	1 cut(s)	321
PspN4I	GGNNCC	2 cut(s)	232, 700
PspPI	GGNCC	2 cut(s)	147, 538
PspPPI	RGGWCCY	1 cut(s)	538
PvuII	CAGCTG	2 cut(s)	193, 260
RsaI	GTAC	2 cut(s)	410, 663
RsaNI	GTAC	2 cut(s)	409, 662
RseI	CAYNNNNRTG	2 cut(s)	645, 669
SapI	GCTCTTC	2 cut(s)	103, 277
SaqAI	TTAA	2 cut(s)	486, 543
SatI	GCNGC	5 cut(s)	34, 94, 142, 530, 624
Sau3AI	GATC	2 cut(s)	128, 631
Sau96I	GGNCC	2 cut(s)	147, 538
ScaI	AGTACT	1 cut(s)	410
SchI	GAGTC	2 cut(s)	419, 506
ScrFI	CCNGG	1 cut(s)	323
SfaNI	GCATC	5 cut(s)	466, 553, 562, 595, 637
SfoI	GGCGCC	1 cut(s)	700
SfuI	TTCGAA	2 cut(s)	393, 763
SinI	GGWCC	1 cut(s)	538
SmiMI	CAYNNNNRTG	2 cut(s)	645, 669
SmlI	CTYRAG	2 cut(s)	520, 533
SmoI	CTYRAG	2 cut(s)	520, 533
Sse9I	AATT	3 cut(s)	505, 681, 759
SsiI	CCGC	5 cut(s)	33, 120, 142, 517, 686
SspDI	GGCGCC	1 cut(s)	698
SspMI	CTAG	1 cut(s)	737
StyD4I	CCNGG	1 cut(s)	321
TaaI	ACNGT	1 cut(s)	242
TaiI	ACGT	1 cut(s)	75
TaqI	TCGA	7 cut(s)	207, 384, 393, 503, 510, 717, 763
TasI	AATT	3 cut(s)	505, 681, 759
TatI	WGTACW	2 cut(s)	408, 661
TauI	GCSGC	2 cut(s)	36, 144
TfiI	GAWTC	1 cut(s)	714
Tru1I	TTAA	2 cut(s)	486, 543
Tru9I	TTAA	2 cut(s)	486, 543
TscAI	CASTG	2 cut(s)	165, 373
TseFI	GTSAC	3 cut(s)	244, 422, 671
TseI	GCWGC	3 cut(s)	93, 529, 623
Tsp45I	GTSAC	3 cut(s)	244, 422, 671
TspDTI	ATGAA	3 cut(s)	468, 609, 629
TspGWI	ACGGA	1 cut(s)	97
TspRI	CASTG	2 cut(s)	165, 373
VpaK11BI	GGWCC	1 cut(s)	538
XapI	RAATTY	2 cut(s)	505, 759
XbaI	TCTAGA	1 cut(s)	736
XceI	RCATGY	1 cut(s)	668
XmnI	GAANNNNTTC	1 cut(s)	749
XspI	CTAG	1 cut(s)	737
ZrmI	AGTACT	1 cut(s)	410

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.