Prupe.1G272900_v2.0.a1

Haloacid dehalogenase-like hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0007289
Physical Location & Seq
Reverse (-)
27927938 .. 27930302
2365 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G272900.1

Sequence Viewer

Length: 801 bp
ATGGAGGGTTGCTTGATGAGATGCTCTCATGGCAGCGCTCTATGGAAAACCCTCAAACCACTCCATCTGAAGCTTCCTAGGAACTCCATTCGTTGCTCTCCCATGCCCATACACTCTGTTAGAGGAGGAGGAAGAGGATCTGCTCTCAATCGCAAGGCCTATGGCGCATTGTTGTTAGACGCTGGAGGCACTCTGCTGCAGTTGGCAAAGCCAGTCGAAGAGGTTTACGCTTCAATTGGAACCCAATACGGAGTGACTGCAACTCCACCTGAAATCAAGCAAGGTTTCAAAAGAGCCTTTTCTGCTCCTTGGCCTCAAAATCTTCGTTACCAGGGAGATGGGAGACCATTTTGGAAACTTGTTGTTTCTGAAGCCACTGGTTGTGCTGATCTCGATTATTTCGAGCAAGTCTATGAGTACTATGCCAAAGGTGACTCATGGCGTCTTCCAGAGGGAGCTTATGAAACAATGGCCCTTCTCAAGGATGCTGGAGTTAAGCTGGCTGTTGTATCCAATTTTGACTCCCGCTTAAGGAAGCTACTCGAAGACCTTAATGTTTTACATCTGTTTGATGCTGTTATCATATCTTCAGAGGTTGGGTATGAAAAACCAGATGCAAACATATTTAAATCTGCTTTAGATCAAGTTCATGTGGATGCTGAAAAAGCTGTGCATGTTGGCGATGATAAAATCGCAGATAAAGTTGGGGCCAATGCCGTTGGAATTGATTGCTGGTTATGGGGCCTGGATGTAAAGACATTTGCCGACATTCGAAATCGCATTCTCATCTCAGAGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2

Protein Analysis

267

Amino Acids

29.34

Weight (kDa)

7.0

Isoelectric Point (pI)

28.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 526
AclWI GGATC 1 cut(s) 145
AcuI CTGAAG 3 cut(s) 89, 390, 573
AcyI GRCGYC 1 cut(s) 442
AfaI GTAC 1 cut(s) 419
AfeI AGCGCT 1 cut(s) 37
AfiI CCNNNNNNNGG 2 cut(s) 481, 531
AflII CTTAAG 1 cut(s) 529
AgsI TTSAA 2 cut(s) 234, 289
AjnI CCWGG 2 cut(s) 330, 744
AluBI AGCT 5 cut(s) 73, 458, 499, 538, 668
AluI AGCT 5 cut(s) 73, 458, 499, 538, 668
Alw26I GTCTC 1 cut(s) 337
AlwI GGATC 1 cut(s) 145
Aor51HI AGCGCT 1 cut(s) 37
AoxI GGCC 5 cut(s) 156, 311, 471, 708, 742
ApeKI GCWGC 2 cut(s) 33, 196
AspA2I CCTAGG 1 cut(s) 77
AspLEI GCGC 2 cut(s) 38, 167
AspS9I GGNCC 3 cut(s) 472, 708, 742
AsuHPI GGTGA 1 cut(s) 443
AsuII TTCGAA 1 cut(s) 772
AvrII CCTAGG 1 cut(s) 77
BbsI GAAGAC 2 cut(s) 437, 552
BbvI GCAGC 2 cut(s) 45, 183
BccI CCATC 2 cut(s) 72, 332
BceAI ACGGC 1 cut(s) 701
BciT130I CCWGG 2 cut(s) 332, 746
BciVI GTATCC 1 cut(s) 520
BcoDI GTCTC 1 cut(s) 337
BfaI CTAG 1 cut(s) 78
BfmI CTRYAG 1 cut(s) 197
BfoI RGCGCY 1 cut(s) 39
BfrI CTTAAG 1 cut(s) 529
BfuI GTATCC 1 cut(s) 520
BisI GCNGC 2 cut(s) 34, 197
BlnI CCTAGG 1 cut(s) 77
BlsI GCNGC 2 cut(s) 35, 198
BmcAI AGTACT 1 cut(s) 419
Bme1390I CCNGG 2 cut(s) 332, 746
BmgT120I GGNCC 3 cut(s) 472, 708, 742
BmiI GGNNCC 3 cut(s) 241, 709, 743
BmrFI CCNGG 2 cut(s) 332, 746
BmsI GCATC 5 cut(s) 11, 475, 562, 604, 646
BpiI GAAGAC 2 cut(s) 437, 552
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpmI CTGGAG 2 cut(s) 204, 510
Bpu14I TTCGAA 1 cut(s) 772
BpuEI CTTGAG 1 cut(s) 464
BsaHI GRCGYC 1 cut(s) 442
BsaI GGTCTC 1 cut(s) 337
BsaJI CCNNGG 3 cut(s) 77, 308, 331
BsaXI ACNNNNNCTCC 2 cut(s) 247, 277
Bsc4I CCNNNNNNNGG 2 cut(s) 481, 531
Bse1I ACTGG 2 cut(s) 212, 382
BseBI CCWGG 2 cut(s) 332, 746
BseDI CCNNGG 3 cut(s) 77, 308, 331
BseGI GGATG 3 cut(s) 490, 661, 754
BseLI CCNNNNNNNGG 2 cut(s) 481, 531
BseNI ACTGG 2 cut(s) 212, 382
BseRI GAGGAG 2 cut(s) 138, 141
BseXI GCAGC 2 cut(s) 45, 183
BshFI GGCC 5 cut(s) 158, 313, 473, 710, 744
BslI CCNNNNNNNGG 2 cut(s) 481, 531
BsmAI GTCTC 1 cut(s) 337
BsmI GAATGC 1 cut(s) 780
BsnI GGCC 5 cut(s) 158, 313, 473, 710, 744
Bso31I GGTCTC 1 cut(s) 337
Bsp119I TTCGAA 1 cut(s) 772
Bsp143I GATC 3 cut(s) 137, 388, 640
BspACI CCGC 1 cut(s) 526
BspANI GGCC 5 cut(s) 158, 313, 473, 710, 744
BspLI GGNNCC 3 cut(s) 241, 709, 743
BspMAI CTGCAG 1 cut(s) 201
BspPI GGATC 1 cut(s) 145
BspT104I TTCGAA 1 cut(s) 772
BspTI CTTAAG 1 cut(s) 529
BspTNI GGTCTC 1 cut(s) 337
BsrI ACTGG 2 cut(s) 212, 382
BssECI CCNNGG 3 cut(s) 77, 308, 331
BssMI GATC 3 cut(s) 137, 388, 640
BssNI GRCGYC 1 cut(s) 442
BssT1I CCWWGG 2 cut(s) 77, 308
Bst2UI CCWGG 2 cut(s) 332, 746
Bst6I CTCTTC 2 cut(s) 127, 213
BstACI GRCGYC 1 cut(s) 442
BstAFI CTTAAG 1 cut(s) 529
BstBI TTCGAA 1 cut(s) 772
BstC8I GCNNGC 1 cut(s) 501
BstDEI CTNAG 1 cut(s) 790
BstENI CCTNNNNNAGG 1 cut(s) 479
BstF5I GGATG 3 cut(s) 490, 661, 754
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 2 cut(s) 38, 167
BstKTI GATC 3 cut(s) 140, 391, 643
BstMAI GTCTC 1 cut(s) 337
BstMBI GATC 3 cut(s) 137, 388, 640
BstMWI GCNNNNNNNGC 4 cut(s) 30, 164, 302, 665
BstNI CCWGG 2 cut(s) 332, 746
BstNSI RCATGY 1 cut(s) 677
BstSCI CCNGG 2 cut(s) 330, 744
BstSFI CTRYAG 1 cut(s) 197
BstV1I GCAGC 2 cut(s) 45, 183
BstV2I GAAGAC 2 cut(s) 437, 552
BstX2I RGATCY 1 cut(s) 137
BstXI CCANNNNNNTGG 1 cut(s) 338
BstYI RGATCY 1 cut(s) 137
BsuI GTATCC 1 cut(s) 520
BsuRI GGCC 5 cut(s) 158, 313, 473, 710, 744
BtgZI GCGATG 1 cut(s) 696
BtsCI GGATG 3 cut(s) 490, 661, 754
BtsIMutI CAGTG 1 cut(s) 375
Cac8I GCNNGC 1 cut(s) 501
CfoI GCGC 2 cut(s) 38, 167
Cfr13I GGNCC 3 cut(s) 472, 708, 742
CseI GACGC 2 cut(s) 188, 431
Csp6I GTAC 1 cut(s) 418
CviAII CATG 5 cut(s) 29, 103, 438, 650, 674
CviQI GTAC 1 cut(s) 418
DdeI CTNAG 1 cut(s) 790
DpnI GATC 3 cut(s) 139, 390, 642
DpnII GATC 3 cut(s) 137, 388, 640
DraI TTTAAA 1 cut(s) 628
Eam1104I CTCTTC 2 cut(s) 127, 213
EarI CTCTTC 2 cut(s) 127, 213
Eco130I CCWWGG 2 cut(s) 77, 308
Eco147I AGGCCT 1 cut(s) 158
Eco31I GGTCTC 1 cut(s) 337
Eco47III AGCGCT 1 cut(s) 37
Eco57I CTGAAG 3 cut(s) 89, 390, 573
EcoNI CCTNNNNNAGG 1 cut(s) 479
EcoO109I RGGNCCY 1 cut(s) 742
EcoRII CCWGG 2 cut(s) 330, 744
EcoT14I CCWWGG 2 cut(s) 77, 308
ErhI CCWWGG 2 cut(s) 77, 308
FaeI CATG 5 cut(s) 32, 106, 441, 653, 677
FatI CATG 5 cut(s) 28, 102, 437, 649, 673
FauI CCCGC 1 cut(s) 533
Fnu4HI GCNGC 2 cut(s) 34, 197
FokI GGATG 3 cut(s) 497, 668, 761
Fsp4HI GCNGC 2 cut(s) 34, 197
FspBI CTAG 1 cut(s) 78
GlaI GCGC 2 cut(s) 37, 166
GluI GCNGC 2 cut(s) 34, 197
GsuI CTGGAG 2 cut(s) 204, 510
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 5 cut(s) 158, 313, 473, 710, 744
HgaI GACGC 2 cut(s) 188, 431
HhaI GCGC 2 cut(s) 38, 167
Hin1I GRCGYC 1 cut(s) 442
Hin1II CATG 5 cut(s) 32, 106, 441, 653, 677
Hin6I GCGC 2 cut(s) 36, 165
HinP1I GCGC 2 cut(s) 36, 165
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 3 cut(s) 434, 521, 794
HphI GGTGA 1 cut(s) 443
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 4 cut(s) 69, 370, 592, 793
Hpy188III TCNNGA 2 cut(s) 392, 449
Hpy8I GTNNAC 1 cut(s) 226
HpyAV CCTTC 1 cut(s) 485
HpyCH4V TGCA 4 cut(s) 199, 260, 617, 673
HpyF10VI GCNNNNNNNGC 4 cut(s) 30, 164, 302, 665
HpyF3I CTNAG 1 cut(s) 790
Hsp92I GRCGYC 1 cut(s) 442
Hsp92II CATG 5 cut(s) 32, 106, 441, 653, 677
HspAI GCGC 2 cut(s) 36, 165
Kzo9I GATC 3 cut(s) 137, 388, 640
LmnI GCTCC 2 cut(s) 310, 455
Lsp1109I GCAGC 2 cut(s) 45, 183
LweI GCATC 5 cut(s) 11, 475, 562, 604, 646
MaeI CTAG 1 cut(s) 78
MaeIII GTNAC 3 cut(s) 253, 326, 431
MalI GATC 3 cut(s) 139, 390, 642
MboI GATC 3 cut(s) 137, 388, 640
MboII GAAGA 6 cut(s) 144, 230, 314, 437, 557, 579
MfeI CAATTG 1 cut(s) 234
MflI RGATCY 1 cut(s) 137
MluCI AATT 3 cut(s) 234, 514, 723
MlyI GAGTC 2 cut(s) 428, 515
MmeI TCCRAC 1 cut(s) 700
MseI TTAA 4 cut(s) 495, 530, 552, 627
MslI CAYNNNNRTG 1 cut(s) 654
MspCI CTTAAG 1 cut(s) 529
MspR9I CCNGG 2 cut(s) 332, 746
MunI CAATTG 1 cut(s) 234
Mva1269I GAATGC 1 cut(s) 780
MvaI CCWGG 2 cut(s) 332, 746
MwoI GCNNNNNNNGC 4 cut(s) 30, 164, 302, 665
NdeII GATC 3 cut(s) 137, 388, 640
NlaIII CATG 5 cut(s) 32, 106, 441, 653, 677
NlaIV GGNNCC 3 cut(s) 241, 709, 743
NmuCI GTSAC 2 cut(s) 253, 431
NspI RCATGY 1 cut(s) 677
NspV TTCGAA 1 cut(s) 772
PceI AGGCCT 1 cut(s) 158
PcsI WCGNNNNNNNCGW 1 cut(s) 399
PctI GAATGC 1 cut(s) 780
PkrI GCNGC 2 cut(s) 35, 198
PleI GAGTC 2 cut(s) 428, 515
PpsI GAGTC 2 cut(s) 428, 515
Psp6I CCWGG 2 cut(s) 330, 744
PspGI CCWGG 2 cut(s) 330, 744
PspN4I GGNNCC 3 cut(s) 241, 709, 743
PspPI GGNCC 3 cut(s) 472, 708, 742
PstI CTGCAG 1 cut(s) 201
PsuI RGATCY 1 cut(s) 137
RsaI GTAC 1 cut(s) 419
RsaNI GTAC 1 cut(s) 418
RseI CAYNNNNRTG 1 cut(s) 654
SaqAI TTAA 4 cut(s) 495, 530, 552, 627
SatI GCNGC 2 cut(s) 34, 197
Sau3AI GATC 3 cut(s) 137, 388, 640
Sau96I GGNCC 3 cut(s) 472, 708, 742
ScaI AGTACT 1 cut(s) 419
SchI GAGTC 2 cut(s) 428, 515
ScrFI CCNGG 2 cut(s) 332, 746
SfaNI GCATC 5 cut(s) 11, 475, 562, 604, 646
SfcI CTRYAG 1 cut(s) 197
SfuI TTCGAA 1 cut(s) 772
SmiI ATTTAAAT 1 cut(s) 628
SmiMI CAYNNNNRTG 1 cut(s) 654
SmlI CTYRAG 2 cut(s) 479, 529
SmoI CTYRAG 2 cut(s) 479, 529
Sse9I AATT 3 cut(s) 234, 514, 723
SseBI AGGCCT 1 cut(s) 158
SsiI CCGC 1 cut(s) 526
SspMI CTAG 1 cut(s) 78
StuI AGGCCT 1 cut(s) 158
StyD4I CCNGG 2 cut(s) 330, 744
StyI CCWWGG 2 cut(s) 77, 308
SwaI ATTTAAAT 1 cut(s) 628
TaqI TCGA 5 cut(s) 216, 393, 402, 543, 772
TasI AATT 3 cut(s) 234, 514, 723
TatI WGTACW 1 cut(s) 417
Tru1I TTAA 4 cut(s) 495, 530, 552, 627
Tru9I TTAA 4 cut(s) 495, 530, 552, 627
TscAI CASTG 1 cut(s) 382
TseFI GTSAC 2 cut(s) 253, 431
TseI GCWGC 2 cut(s) 33, 196
Tsp45I GTSAC 2 cut(s) 253, 431
TspDTI ATGAA 3 cut(s) 477, 618, 638
TspGWI ACGGA 1 cut(s) 264
TspRI CASTG 1 cut(s) 382
Vha464I CTTAAG 1 cut(s) 529
XagI CCTNNNNNAGG 1 cut(s) 479
XceI RCATGY 1 cut(s) 677
XmaJI CCTAGG 1 cut(s) 77
XspI CTAG 1 cut(s) 78
ZrmI AGTACT 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.