RLG00000006218

Haloacid dehalogenase-like hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0007289
Physical Location & Seq
Reverse (-)
5317254 .. 5319096
1843 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006218

Sequence Viewer

Length: 783 bp
ATGGAGGGTTGCTTGATCAAATGCTCTCACGGCGGCAGCGCTCTCTTGAAAACCCTTAAACCTCTTCATCCCAAAACGCACATTCGCTTCTCTTCAACGCCAATTCGCTCCGGCGAAGCCCTCCGATCGCCTCGCAGAGCCTACGACGCATTGTTGTTGGACGCCGGAGGTACTCTCTTGCAGCTGGCCAACCCCGTCGAAGAGGTCTACGCCTCCATAGGAACCCAATACGGTGTGACTGCAACTCCAGCCGAAATCAAGCAGGGTTTCAGAAGAGCTTTCTCTGCTCCTTGGCCTCAAAAGCTCCGATACCAGGGAGATGGGAGGCCATTTTGGAAACTAGTTGTTTCAGAAGCCACTGGTTCTTCTGATCTTGATTATTTCGAACACGTCTATCAGTACTATGCTAGAGGTGATTCATGGCGTTTACCCGATGGAGCTTATGAAACAATGGTACTTCTCAAGGATGCTGGAGTTAAGCTTGCTGTTGTATCGAATTTTGACTCCCGCTTAAGGAAACTACTTGAGGAGCTGAATGTGTTGCATCTGTTTGATGCTGTTATCATATCTTCAGAGGTTGGTTCTGAAAAACCAGATGCAAACATATTTAAAGCTGCTTTAGATCAAACTCATGCGGATGCTGGCAAGGCAGTACATGTTGGTGATGATGAAAAAGCAGATAAGATTGGGGCCAATGCTGTTGGAATTGATTGCTGGTTATGGGGCAAGGAGGTGAAGACATTCTCCGACATTCGAAATAAAATTCTCATTTCAGAGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2

Protein Analysis

261

Amino Acids

28.65

Weight (kDa)

6.66

Isoelectric Point (pI)

38.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 48 - 235 1.9e-20 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 126 - 238 1.4e-15 Haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 207
AciI CCGC 3 cut(s) 33, 508, 635
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 2 cut(s) 496, 762
AcuI CTGAAG 1 cut(s) 555
AcyI GRCGYC 1 cut(s) 162
AfaI GTAC 4 cut(s) 172, 401, 456, 654
AfeI AGCGCT 1 cut(s) 40
AfiI CCNNNNNNNGG 2 cut(s) 313, 513
AflII CTTAAG 1 cut(s) 511
AflIII ACRYGT 2 cut(s) 388, 655
AgsI TTSAA 2 cut(s) 49, 96
AhlI ACTAGT 1 cut(s) 340
AjiI CACGTC 1 cut(s) 391
AjnI CCWGG 1 cut(s) 312
AluBI AGCT 7 cut(s) 184, 278, 304, 440, 481, 532, 614
AluI AGCT 7 cut(s) 184, 278, 304, 440, 481, 532, 614
Aor51HI AGCGCT 1 cut(s) 40
AoxI GGCC 4 cut(s) 186, 293, 326, 690
ApeKI GCWGC 3 cut(s) 36, 181, 614
ApoI RAATTY 2 cut(s) 496, 762
Asp700I GAANNNNTTC 1 cut(s) 740
AspLEI GCGC 1 cut(s) 41
AspS9I GGNCC 1 cut(s) 690
AsuHPI GGTGA 3 cut(s) 425, 674, 745
AsuII TTCGAA 2 cut(s) 384, 754
BalI TGGCCA 1 cut(s) 188
BbsI GAAGAC 1 cut(s) 743
BbvI GCAGC 3 cut(s) 48, 193, 601
BccI CCATC 2 cut(s) 314, 428
BceAI ACGGC 1 cut(s) 46
BciT130I CCWGG 1 cut(s) 314
BclI TGATCA 1 cut(s) 15
BcuI ACTAGT 1 cut(s) 340
BfaI CTAG 2 cut(s) 341, 408
BfoI RGCGCY 1 cut(s) 42
BfrI CTTAAG 1 cut(s) 511
BisI GCNGC 4 cut(s) 34, 37, 182, 615
BlsI GCNGC 4 cut(s) 35, 38, 183, 616
BmcAI AGTACT 1 cut(s) 401
Bme1390I CCNGG 1 cut(s) 314
BmgBI CACGTC 1 cut(s) 391
BmgT120I GGNCC 1 cut(s) 690
BmiI GGNNCC 2 cut(s) 223, 691
BmrFI CCNGG 1 cut(s) 314
BmsI GCATC 5 cut(s) 457, 544, 553, 586, 628
BpiI GAAGAC 1 cut(s) 743
BplI GAGNNNNNCTC 2 cut(s) 159, 191
BpmI CTGGAG 2 cut(s) 231, 492
Bpu14I TTCGAA 2 cut(s) 384, 754
BpuEI CTTGAG 2 cut(s) 446, 545
BsaHI GRCGYC 1 cut(s) 162
BsaJI CCNNGG 2 cut(s) 290, 313
BsaXI ACNNNNNCTCC 2 cut(s) 229, 259
Bsc4I CCNNNNNNNGG 2 cut(s) 313, 513
Bse1I ACTGG 1 cut(s) 364
BseBI CCWGG 1 cut(s) 314
BseDI CCNNGG 2 cut(s) 290, 313
BseGI GGATG 3 cut(s) 67, 472, 643
BseLI CCNNNNNNNGG 2 cut(s) 313, 513
BseNI ACTGG 1 cut(s) 364
BseRI GAGGAG 1 cut(s) 542
BseXI GCAGC 3 cut(s) 48, 193, 601
Bsh1285I CGRYCG 1 cut(s) 128
BshFI GGCC 4 cut(s) 188, 295, 328, 692
BsiEI CGRYCG 1 cut(s) 128
BsiSI CCGG 2 cut(s) 111, 165
BslI CCNNNNNNNGG 2 cut(s) 313, 513
BsnI GGCC 4 cut(s) 188, 295, 328, 692
Bsp119I TTCGAA 2 cut(s) 384, 754
Bsp143I GATC 4 cut(s) 15, 125, 370, 622
BspACI CCGC 3 cut(s) 33, 508, 635
BspANI GGCC 4 cut(s) 188, 295, 328, 692
BspLI GGNNCC 2 cut(s) 223, 691
BspQI GCTCTTC 1 cut(s) 268
BspT104I TTCGAA 2 cut(s) 384, 754
BspTI CTTAAG 1 cut(s) 511
BsrI ACTGG 1 cut(s) 364
BssECI CCNNGG 2 cut(s) 290, 313
BssMI GATC 4 cut(s) 15, 125, 370, 622
BssNI GRCGYC 1 cut(s) 162
BssT1I CCWWGG 1 cut(s) 290
Bst2UI CCWGG 1 cut(s) 314
Bst4CI ACNGT 1 cut(s) 233
Bst6I CTCTTC 4 cut(s) 69, 97, 195, 268
BstACI GRCGYC 1 cut(s) 162
BstAFI CTTAAG 1 cut(s) 511
BstBI TTCGAA 2 cut(s) 384, 754
BstC8I GCNNGC 3 cut(s) 186, 483, 643
BstF5I GGATG 3 cut(s) 67, 472, 643
BstH2I RGCGCY 1 cut(s) 42
BstHHI GCGC 1 cut(s) 41
BstKTI GATC 4 cut(s) 18, 128, 373, 625
BstMBI GATC 4 cut(s) 15, 125, 370, 622
BstMCI CGRYCG 1 cut(s) 128
BstMWI GCNNNNNNNGC 6 cut(s) 30, 146, 248, 284, 301, 647
BstNI CCWGG 1 cut(s) 314
BstNSI RCATGY 1 cut(s) 659
BstSCI CCNGG 1 cut(s) 312
BstV1I GCAGC 3 cut(s) 48, 193, 601
BstV2I GAAGAC 1 cut(s) 743
BstXI CCANNNNNNTGG 1 cut(s) 320
BsuRI GGCC 4 cut(s) 188, 295, 328, 692
BtrI CACGTC 1 cut(s) 391
BtsCI GGATG 3 cut(s) 67, 472, 643
BtsIMutI CAGTG 1 cut(s) 357
Cac8I GCNNGC 3 cut(s) 186, 483, 643
CfoI GCGC 1 cut(s) 41
Cfr13I GGNCC 1 cut(s) 690
CseI GACGC 2 cut(s) 155, 170
Csp6I GTAC 4 cut(s) 171, 400, 455, 653
CviAII CATG 3 cut(s) 420, 632, 656
CviQI GTAC 4 cut(s) 171, 400, 455, 653
DpnI GATC 4 cut(s) 17, 127, 372, 624
DpnII GATC 4 cut(s) 15, 125, 370, 622
DraI TTTAAA 1 cut(s) 610
EaeI YGGCCR 1 cut(s) 186
Eam1104I CTCTTC 4 cut(s) 69, 97, 195, 268
EarI CTCTTC 4 cut(s) 69, 97, 195, 268
Eco130I CCWWGG 1 cut(s) 290
Eco47III AGCGCT 1 cut(s) 40
Eco57I CTGAAG 1 cut(s) 555
EcoRII CCWGG 1 cut(s) 312
EcoT14I CCWWGG 1 cut(s) 290
ErhI CCWWGG 1 cut(s) 290
FaeI CATG 3 cut(s) 423, 635, 659
FatI CATG 3 cut(s) 419, 631, 655
FauI CCCGC 1 cut(s) 515
FbaI TGATCA 1 cut(s) 15
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 4 cut(s) 34, 37, 182, 615
FokI GGATG 3 cut(s) 54, 479, 650
Fsp4HI GCNGC 4 cut(s) 34, 37, 182, 615
FspBI CTAG 2 cut(s) 341, 408
GlaI GCGC 1 cut(s) 40
GluI GCNGC 4 cut(s) 34, 37, 182, 615
GsuI CTGGAG 2 cut(s) 231, 492
HaeII RGCGCY 1 cut(s) 42
HaeIII GGCC 4 cut(s) 188, 295, 328, 692
HapII CCGG 2 cut(s) 111, 165
HgaI GACGC 2 cut(s) 155, 170
HhaI GCGC 1 cut(s) 41
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 3 cut(s) 423, 635, 659
Hin6I GCGC 1 cut(s) 39
HinP1I GCGC 1 cut(s) 39
HindIII AAGCTT 1 cut(s) 479
HinfI GANTC 3 cut(s) 416, 503, 776
HpaII CCGG 2 cut(s) 111, 165
HphI GGTGA 3 cut(s) 425, 674, 745
Hpy166II GTNNAC 2 cut(s) 208, 428
Hpy188I TCNGA 9 cut(s) 125, 272, 308, 352, 370, 574, 586, 748, 775
Hpy188III TCNNGA 2 cut(s) 46, 374
Hpy8I GTNNAC 2 cut(s) 208, 428
Hpy99I CGWCG 2 cut(s) 149, 200
HpyCH4III ACNGT 1 cut(s) 233
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 4 cut(s) 181, 242, 544, 599
HpyF10VI GCNNNNNNNGC 6 cut(s) 30, 146, 248, 284, 301, 647
HpySE526I ACGT 1 cut(s) 390
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 3 cut(s) 423, 635, 659
HspAI GCGC 1 cut(s) 39
Ksp22I TGATCA 1 cut(s) 15
Kzo9I GATC 4 cut(s) 15, 125, 370, 622
LguI GCTCTTC 1 cut(s) 268
LmnI GCTCC 5 cut(s) 113, 292, 309, 437, 529
Lsp1109I GCAGC 3 cut(s) 48, 193, 601
LweI GCATC 5 cut(s) 457, 544, 553, 586, 628
MaeI CTAG 2 cut(s) 341, 408
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 1 cut(s) 235
MalI GATC 4 cut(s) 17, 127, 372, 624
MboI GATC 4 cut(s) 15, 125, 370, 622
MboII GAAGA 7 cut(s) 56, 84, 212, 285, 357, 561, 748
MlsI TGGCCA 1 cut(s) 188
MluCI AATT 4 cut(s) 102, 496, 705, 762
MluNI TGGCCA 1 cut(s) 188
MlyI GAGTC 1 cut(s) 497
MmeI TCCRAC 3 cut(s) 138, 682, 771
Mox20I TGGCCA 1 cut(s) 188
MroXI GAANNNNTTC 1 cut(s) 740
MscI TGGCCA 1 cut(s) 188
MseI TTAA 4 cut(s) 57, 477, 512, 609
MslI CAYNNNNRTG 2 cut(s) 636, 660
Msp20I TGGCCA 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 184
MspCI CTTAAG 1 cut(s) 511
MspI CCGG 2 cut(s) 111, 165
MspR9I CCNGG 1 cut(s) 314
MvaI CCWGG 1 cut(s) 314
MwoI GCNNNNNNNGC 6 cut(s) 30, 146, 248, 284, 301, 647
NdeII GATC 4 cut(s) 15, 125, 370, 622
NlaIII CATG 3 cut(s) 423, 635, 659
NlaIV GGNNCC 2 cut(s) 223, 691
NmuCI GTSAC 1 cut(s) 235
NspI RCATGY 1 cut(s) 659
NspV TTCGAA 2 cut(s) 384, 754
PciI ACATGT 1 cut(s) 655
PciSI GCTCTTC 1 cut(s) 268
PdmI GAANNNNTTC 1 cut(s) 740
PfeI GAWTC 1 cut(s) 416
PkrI GCNGC 4 cut(s) 35, 38, 183, 616
Ple19I CGATCG 1 cut(s) 128
PleI GAGTC 1 cut(s) 497
PpsI GAGTC 1 cut(s) 497
PscI ACATGT 1 cut(s) 655
Psp6I CCWGG 1 cut(s) 312
PspGI CCWGG 1 cut(s) 312
PspN4I GGNNCC 2 cut(s) 223, 691
PspPI GGNCC 1 cut(s) 690
PvuI CGATCG 1 cut(s) 128
PvuII CAGCTG 1 cut(s) 184
RsaI GTAC 4 cut(s) 172, 401, 456, 654
RsaNI GTAC 4 cut(s) 171, 400, 455, 653
RseI CAYNNNNRTG 2 cut(s) 636, 660
SapI GCTCTTC 1 cut(s) 268
SaqAI TTAA 4 cut(s) 57, 477, 512, 609
SatI GCNGC 4 cut(s) 34, 37, 182, 615
Sau3AI GATC 4 cut(s) 15, 125, 370, 622
Sau96I GGNCC 1 cut(s) 690
ScaI AGTACT 1 cut(s) 401
SchI GAGTC 1 cut(s) 497
ScrFI CCNGG 1 cut(s) 314
SfaNI GCATC 5 cut(s) 457, 544, 553, 586, 628
SfuI TTCGAA 2 cut(s) 384, 754
SmiMI CAYNNNNRTG 2 cut(s) 636, 660
SmlI CTYRAG 3 cut(s) 461, 511, 524
SmoI CTYRAG 3 cut(s) 461, 511, 524
SpeI ACTAGT 1 cut(s) 340
Sse9I AATT 4 cut(s) 102, 496, 705, 762
SsiI CCGC 3 cut(s) 33, 508, 635
SspMI CTAG 2 cut(s) 341, 408
StyD4I CCNGG 1 cut(s) 312
StyI CCWWGG 1 cut(s) 290
TaaI ACNGT 1 cut(s) 233
TaiI ACGT 1 cut(s) 393
TaqI TCGA 4 cut(s) 198, 384, 494, 754
TasI AATT 4 cut(s) 102, 496, 705, 762
TatI WGTACW 2 cut(s) 399, 652
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 1 cut(s) 416
Tru1I TTAA 4 cut(s) 57, 477, 512, 609
Tru9I TTAA 4 cut(s) 57, 477, 512, 609
TscAI CASTG 1 cut(s) 364
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 3 cut(s) 36, 181, 614
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 4 cut(s) 56, 408, 459, 684
TspRI CASTG 1 cut(s) 364
Vha464I CTTAAG 1 cut(s) 511
XapI RAATTY 2 cut(s) 496, 762
XceI RCATGY 1 cut(s) 659
XmiI GTMKAC 1 cut(s) 207
XmnI GAANNNNTTC 1 cut(s) 740
XspI CTAG 2 cut(s) 341, 408
ZrmI AGTACT 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.