AT1G47570

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003425
Physical Location & Seq
Forward (+)
17477579 .. 17483502
5924 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G47570.2

Sequence Viewer

Length: 1401 bp
ATGACGACGACGGCGGAAACTGGGCAATCCTCTGGTTCGAAACCCTCTGATGATGATGCATGGGCCAAACTGGTTCCTCTGGACACCAGATTCTCTGACATTGAGATTAGGTGCAATGATATGGTAATATGCTCTGAGATTAAACCGTCTTCCCTGGAAAAACATGAATGGTGCAGAATAACTAAGAATTTGGGTCAAAGTTCTGCCACTATACATAATAAGAGTTCGGATGCTATACTTGTTGACAAGGCTGTTGTCCCGAAAGATGGTGCTGTTGATATCATTAGTGGATCTGAAATTGTTCCTGGCCCTGAAGAACAAGGGTACTTGCAGTACAGATTTACAATAATGCCTGCTCCAGAATCCAGAACCCAGCTATTACAGATTTCCATAGATCCTGAACATGCAAAATGCAGTATCTGCTTAAACATATGGCATGATGTTGTCACAGCTGCACCTTGCCTTCATAATTTCTGTAATGGCTGTTTTTCAGAATGGATGCGGAGATCGGAGGAAAAACATAAACATGTGTTATGTCCACAGTGCAGAACAACAGTACAATATGTGGGAAAAAATCATTTTCTGAAGAACATACAAGAGGAAATATTAAAAGTGGATGCTGCACTGAGGCGACCAGCTGAAGATATTGCAGTGTTGGACTCATCTGCATCGATACAATCAAATCTTATCATTGGGAGCAAGAGAAAACGTCGATTGAATATGCCTGCGCCCACACATGAAGAAAGAGATAGCTTGCGTTTGCAATGCCCACAATGTGTTGCTAATATTGGTGGTTATCGGTGTGAACATCATGGTGCACATCTGCAATGCCACCTGTGTCAAGGAATGATGCCCTTTAGAGCCAATCTCCAAGTGCCGTTACACTGCAAGGGATGCGATAGGCCGTTTTGCGGTGCTTACTGGAGTTCCGAGAATGTTACACAAGGTGTATCTGGTCCAGTCTGCGTTCGCGAAACTTTTAGACCTATCTCGGAACGCACAATTACAAGGATTCCATTCATAACCCATGAAATGAATCGCCATGAACAAGATATAACGCAAAGATGCATTGCCCATATGGAGAAAACTGTGCCGGATGTTGTAGCAGAGTGGCTCAGGCTTTTCAACAATAGAGAAATAGACCGCTCGAGAATGCCTCTGAATCATGCTGAGACGATAACTGCATCAACACATGTTTGCAATGATTGTTATGATAAGCTGGTGGGATTCCTTTTATACTGGTTCCGGATAACACTGCCGAGAAATCATTTGCCAGCGGATGTAGCGGCTAGGGAAGATTGTTGGTATGGATACGCATGTAGGACACAACACCACAACGAAGATCATGCTCGTAAACGTAACCATGTATGTCGCCCTACCAGAGGGAACCATCACTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000209 GO:0000278 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016604 GO:0016605 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031647 GO:0031648 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0036094 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045732 GO:0045786 GO:0045862 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051726 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0097159 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1903047 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_2

Protein Analysis

466

Amino Acids

53.1

Weight (kDa)

7.54

Isoelectric Point (pI)

56.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 124 - 170 1.8e-06 Ring finger domain
zf-C3HC4_2 PF13923 124 - 169 3.3e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 125 - 169 5.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-CRD PF17979 243 - 405 3.1e-47 Cysteine rich domain with multizinc binding regions
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1146
AccII CGCG 1 cut(s) 972
AccIII TCCGGA 1 cut(s) 1245
AciI CCGC 6 cut(s) 14, 502, 912, 1144, 1277, 1286
AclWI GGATC 2 cut(s) 298, 389
AcsI RAATTY 1 cut(s) 187
AcuI CTGAAG 3 cut(s) 333, 605, 660
AdeI CACNNNGTG 2 cut(s) 776, 947
AfaI GTAC 3 cut(s) 326, 335, 558
AfiI CCNNNNNNNGG 3 cut(s) 266, 911, 1382
AflIII ACRYGT 2 cut(s) 526, 1192
AgsI TTSAA 2 cut(s) 718, 1126
AjnI CCWGG 2 cut(s) 153, 304
AluBI AGCT 5 cut(s) 376, 452, 638, 753, 1219
AluI AGCT 5 cut(s) 376, 452, 638, 753, 1219
Alw21I GWGCWC 1 cut(s) 820
Alw26I GTCTC 1 cut(s) 1166
Alw44I GTGCAC 1 cut(s) 816
AlwI GGATC 2 cut(s) 298, 389
AlwNI CAGNNNCTG 1 cut(s) 420
Ama87I CYCGRG 1 cut(s) 1147
Aor13HI TCCGGA 1 cut(s) 1245
AoxI GGCC 3 cut(s) 63, 307, 902
ApaLI GTGCAC 1 cut(s) 816
ApeKI GCWGC 2 cut(s) 452, 620
ApoI RAATTY 1 cut(s) 187
Asp700I GAANNNNTTC 1 cut(s) 300
AspLEI GCGC 1 cut(s) 730
AspS9I GGNCC 3 cut(s) 63, 308, 956
AsuII TTCGAA 1 cut(s) 38
AvaI CYCGRG 1 cut(s) 1147
AvaII GGWCC 1 cut(s) 956
BaeGI GKGCMC 1 cut(s) 820
BaeI ACNNNNGTAYC 2 cut(s) 1303, 1336
BbsI GAAGAC 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 820
BbvI GCAGC 2 cut(s) 439, 607
BccI CCATC 2 cut(s) 260, 1398
BceAI ACGGC 3 cut(s) 27, 862, 889
BcgI CGANNNNNNTGC 2 cut(s) 1328, 1362
BciT130I CCWGG 2 cut(s) 155, 306
BciVI GTATCC 1 cut(s) 1304
BcoDI GTCTC 1 cut(s) 1166
BfaI CTAG 1 cut(s) 1290
BfuI GTATCC 1 cut(s) 1304
BisI GCNGC 3 cut(s) 453, 621, 1287
BlsI GCNGC 3 cut(s) 454, 622, 1288
Bme1390I CCNGG 2 cut(s) 155, 306
Bme18I GGWCC 1 cut(s) 956
BmeT110I CYCGRG 1 cut(s) 1147
BmgT120I GGNCC 3 cut(s) 63, 308, 956
BmiI GGNNCC 3 cut(s) 75, 1244, 1388
BmrFI CCNGG 2 cut(s) 155, 306
BmrI ACTGGG 1 cut(s) 30
BmsI GCATC 9 cut(s) 46, 220, 489, 607, 677, 840, 884, 1055, 1193
BmuI ACTGGG 1 cut(s) 30
BpiI GAAGAC 1 cut(s) 141
BplI GAGNNNNNCTC 4 cut(s) 852, 884, 1141, 1173
BpmI CTGGAG 2 cut(s) 342, 943
Bpu10I CCTNAGC 1 cut(s) 1115
Bpu14I TTCGAA 1 cut(s) 38
Bsa29I ATCGAT 1 cut(s) 671
BsaJI CCNNGG 1 cut(s) 153
BsaWI WCCGGW 1 cut(s) 1245
Bsc4I CCNNNNNNNGG 3 cut(s) 266, 911, 1382
Bse1I ACTGG 5 cut(s) 25, 75, 926, 959, 1244
Bse3DI GCAATG 5 cut(s) 121, 770, 833, 1068, 1207
BseAI TCCGGA 1 cut(s) 1245
BseBI CCWGG 2 cut(s) 155, 306
BseCI ATCGAT 1 cut(s) 671
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 6 cut(s) 235, 504, 622, 899, 1102, 1285
BseLI CCNNNNNNNGG 3 cut(s) 266, 911, 1382
BseMI GCAATG 5 cut(s) 121, 770, 833, 1068, 1207
BseMII CTCAG 4 cut(s) 126, 617, 1129, 1161
BseNI ACTGG 5 cut(s) 25, 75, 926, 959, 1244
BseSI GKGCMC 1 cut(s) 820
BseXI GCAGC 2 cut(s) 439, 607
BseYI CCCAGC 1 cut(s) 372
BsgI GTGCAG 4 cut(s) 193, 438, 565, 606
Bsh1236I CGCG 1 cut(s) 972
BshFI GGCC 3 cut(s) 65, 309, 904
BshVI ATCGAT 1 cut(s) 671
BsiHKAI GWGCWC 1 cut(s) 820
BsiHKCI CYCGRG 1 cut(s) 1147
BsiSI CCGG 2 cut(s) 1094, 1246
BslFI GGGAC 1 cut(s) 242
BslI CCNNNNNNNGG 3 cut(s) 266, 911, 1382
BsmAI GTCTC 1 cut(s) 1166
BsmBI CGTCTC 1 cut(s) 1166
BsmFI GGGAC 1 cut(s) 242
BsmI GAATGC 1 cut(s) 1158
BsnI GGCC 3 cut(s) 65, 309, 904
BsoBI CYCGRG 1 cut(s) 1147
Bsp119I TTCGAA 1 cut(s) 38
Bsp1286I GDGCHC 1 cut(s) 820
Bsp13I TCCGGA 1 cut(s) 1245
Bsp143I GATC 4 cut(s) 290, 394, 506, 1342
Bsp68I TCGCGA 1 cut(s) 972
BspACI CCGC 6 cut(s) 14, 502, 912, 1144, 1277, 1286
BspANI GGCC 3 cut(s) 65, 309, 904
BspCNI CTCAG 4 cut(s) 127, 618, 1128, 1162
BspDI ATCGAT 1 cut(s) 671
BspEI TCCGGA 1 cut(s) 1245
BspFNI CGCG 1 cut(s) 972
BspLI GGNNCC 3 cut(s) 75, 1244, 1388
BspPI GGATC 2 cut(s) 298, 389
BspT104I TTCGAA 1 cut(s) 38
BsrBI CCGCTC 1 cut(s) 1146
BsrDI GCAATG 5 cut(s) 121, 770, 833, 1068, 1207
BsrI ACTGG 5 cut(s) 25, 75, 926, 959, 1244
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 4 cut(s) 290, 394, 506, 1342
Bst2UI CCWGG 2 cut(s) 155, 306
Bst4CI ACNGT 4 cut(s) 147, 543, 556, 1090
BstAPI GCANNNNNTGC 2 cut(s) 420, 894
BstBI TTCGAA 1 cut(s) 38
BstC8I GCNNGC 4 cut(s) 354, 726, 755, 1275
BstDEI CTNAG 5 cut(s) 135, 183, 626, 1115, 1170
BstENI CCTNNNNNAGG 1 cut(s) 1380
BstF5I GGATG 6 cut(s) 235, 504, 622, 899, 1102, 1285
BstFNI CGCG 1 cut(s) 972
BstHHI GCGC 1 cut(s) 730
BstKTI GATC 4 cut(s) 293, 397, 509, 1345
BstMAI GTCTC 1 cut(s) 1166
BstMBI GATC 4 cut(s) 290, 394, 506, 1342
BstMWI GCNNNNNNNGC 3 cut(s) 420, 894, 1283
BstNI CCWGG 2 cut(s) 155, 306
BstNSI RCATGY 4 cut(s) 407, 530, 1196, 1320
BstSCI CCNGG 2 cut(s) 153, 304
BstSLI GKGCMC 1 cut(s) 820
BstUI CGCG 1 cut(s) 972
BstV1I GCAGC 2 cut(s) 439, 607
BstV2I GAAGAC 1 cut(s) 141
BstX2I RGATCY 2 cut(s) 290, 394
BstYI RGATCY 2 cut(s) 290, 394
Bsu15I ATCGAT 1 cut(s) 671
BsuI GTATCC 1 cut(s) 1304
BsuRI GGCC 3 cut(s) 65, 309, 904
BsuTUI ATCGAT 1 cut(s) 671
BtsCI GGATG 6 cut(s) 235, 504, 622, 899, 1102, 1285
BtsI GCAGTG 3 cut(s) 657, 883, 1253
BtsIMutI CAGTG 5 cut(s) 548, 623, 657, 883, 1253
BtuMI TCGCGA 1 cut(s) 972
Cac8I GCNNGC 4 cut(s) 354, 726, 755, 1275
CaiI CAGNNNCTG 1 cut(s) 420
CfoI GCGC 1 cut(s) 730
Cfr13I GGNCC 3 cut(s) 63, 308, 956
ClaI ATCGAT 1 cut(s) 671
Csp6I GTAC 3 cut(s) 325, 334, 557
CviQI GTAC 3 cut(s) 325, 334, 557
DdeI CTNAG 5 cut(s) 135, 183, 626, 1115, 1170
DpnI GATC 4 cut(s) 292, 396, 508, 1344
DpnII GATC 4 cut(s) 290, 394, 506, 1342
DraIII CACNNNGTG 2 cut(s) 776, 947
EciI GGCGGA 1 cut(s) 29
Eco32I GATATC 1 cut(s) 280
Eco47I GGWCC 1 cut(s) 956
Eco57I CTGAAG 3 cut(s) 333, 605, 660
Eco88I CYCGRG 1 cut(s) 1147
EcoNI CCTNNNNNAGG 1 cut(s) 1380
EcoRII CCWGG 2 cut(s) 153, 304
EcoRV GATATC 1 cut(s) 280
EcoT22I ATGCAT 2 cut(s) 61, 1070
Esp3I CGTCTC 1 cut(s) 1166
FaqI GGGAC 1 cut(s) 242
FauNDI CATATG 2 cut(s) 431, 1077
Fnu4HI GCNGC 3 cut(s) 453, 621, 1287
FokI GGATG 6 cut(s) 242, 511, 629, 906, 1109, 1292
Fsp4HI GCNGC 3 cut(s) 453, 621, 1287
FspBI CTAG 1 cut(s) 1290
GlaI GCGC 1 cut(s) 729
GluI GCNGC 3 cut(s) 453, 621, 1287
GsaI CCCAGC 1 cut(s) 376
GsuI CTGGAG 2 cut(s) 342, 943
HaeIII GGCC 3 cut(s) 65, 309, 904
HapII CCGG 2 cut(s) 1094, 1246
HhaI GCGC 1 cut(s) 730
Hin6I GCGC 1 cut(s) 728
HinP1I GCGC 1 cut(s) 728
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HinfI GANTC 7 cut(s) 90, 362, 659, 1012, 1036, 1162, 1227
HpaII CCGG 2 cut(s) 1094, 1246
Hpy166II GTNNAC 5 cut(s) 244, 539, 806, 818, 1355
Hpy188III TCNNGA 8 cut(s) 80, 259, 359, 366, 398, 971, 1149, 1246
Hpy8I GTNNAC 5 cut(s) 244, 539, 806, 818, 1355
Hpy99I CGWCG 3 cut(s) 10, 13, 714
HpyAV CCTTC 1 cut(s) 473
HpyCH4III ACNGT 4 cut(s) 147, 543, 556, 1090
HpyCH4IV ACGT 2 cut(s) 709, 1357
HpyF10VI GCNNNNNNNGC 3 cut(s) 420, 894, 1283
HpyF3I CTNAG 5 cut(s) 135, 183, 626, 1115, 1170
HpySE526I ACGT 2 cut(s) 709, 1357
HspAI GCGC 1 cut(s) 728
Kpn2I TCCGGA 1 cut(s) 1245
Kzo9I GATC 4 cut(s) 290, 394, 506, 1342
LmnI GCTCC 2 cut(s) 361, 696
Lsp1109I GCAGC 2 cut(s) 439, 607
LweI GCATC 9 cut(s) 46, 220, 489, 607, 677, 840, 884, 1055, 1193
MaeI CTAG 1 cut(s) 1290
MaeII ACGT 2 cut(s) 709, 1357
MaeIII GTNAC 4 cut(s) 445, 879, 937, 1358
MalI GATC 4 cut(s) 292, 396, 508, 1344
MbiI CCGCTC 1 cut(s) 1146
MboI GATC 4 cut(s) 290, 394, 506, 1342
MboII GAAGA 7 cut(s) 141, 326, 598, 653, 752, 1307, 1352
MflI RGATCY 2 cut(s) 290, 394
MhlI GDGCHC 1 cut(s) 820
MluCI AATT 4 cut(s) 187, 297, 469, 1002
MlyI GAGTC 1 cut(s) 653
MmeI TCCRAC 1 cut(s) 636
MnlI CCTC 8 cut(s) 40, 55, 87, 505, 592, 621, 1167, 1376
Mph1103I ATGCAT 2 cut(s) 61, 1070
MroI TCCGGA 1 cut(s) 1245
MroXI GAANNNNTTC 1 cut(s) 300
MseI TTAA 3 cut(s) 141, 425, 608
MslI CAYNNNNRTG 2 cut(s) 525, 813
MspA1I CMGCKG 3 cut(s) 452, 638, 1277
MspI CCGG 2 cut(s) 1094, 1246
MspR9I CCNGG 2 cut(s) 155, 306
Mva1269I GAATGC 1 cut(s) 1158
MvaI CCWGG 2 cut(s) 155, 306
MvnI CGCG 1 cut(s) 972
MwoI GCNNNNNNNGC 3 cut(s) 420, 894, 1283
NdeI CATATG 2 cut(s) 431, 1077
NdeII GATC 4 cut(s) 290, 394, 506, 1342
NlaIV GGNNCC 3 cut(s) 75, 1244, 1388
NmeAIII GCCGAG 1 cut(s) 1284
NmuCI GTSAC 1 cut(s) 445
NruI TCGCGA 1 cut(s) 972
NsiI ATGCAT 2 cut(s) 61, 1070
NspI RCATGY 4 cut(s) 407, 530, 1196, 1320
NspV TTCGAA 1 cut(s) 38
PaeR7I CTCGAG 1 cut(s) 1147
PciI ACATGT 2 cut(s) 526, 1192
PctI GAATGC 1 cut(s) 1158
PdmI GAANNNNTTC 1 cut(s) 300
PfeI GAWTC 6 cut(s) 90, 362, 1012, 1036, 1162, 1227
PkrI GCNGC 3 cut(s) 454, 622, 1288
PleI GAGTC 1 cut(s) 653
PpsI GAGTC 1 cut(s) 653
PscI ACATGT 2 cut(s) 526, 1192
Psp6I CCWGG 2 cut(s) 153, 304
PspFI CCCAGC 1 cut(s) 372
PspGI CCWGG 2 cut(s) 153, 304
PspN4I GGNNCC 3 cut(s) 75, 1244, 1388
PspPI GGNCC 3 cut(s) 63, 308, 956
PstNI CAGNNNCTG 1 cut(s) 420
PsuI RGATCY 2 cut(s) 290, 394
PvuII CAGCTG 2 cut(s) 452, 638
RruI TCGCGA 1 cut(s) 972
RsaI GTAC 3 cut(s) 326, 335, 558
RsaNI GTAC 3 cut(s) 325, 334, 557
RseI CAYNNNNRTG 2 cut(s) 525, 813
SaqAI TTAA 3 cut(s) 141, 425, 608
SatI GCNGC 3 cut(s) 453, 621, 1287
Sau3AI GATC 4 cut(s) 290, 394, 506, 1342
Sau96I GGNCC 3 cut(s) 63, 308, 956
SchI GAGTC 1 cut(s) 653
ScrFI CCNGG 2 cut(s) 155, 306
SduI GDGCHC 1 cut(s) 820
SfaNI GCATC 9 cut(s) 46, 220, 489, 607, 677, 840, 884, 1055, 1193
Sfr274I CTCGAG 1 cut(s) 1147
SfuI TTCGAA 1 cut(s) 38
SinI GGWCC 1 cut(s) 956
SlaI CTCGAG 1 cut(s) 1147
SmiMI CAYNNNNRTG 2 cut(s) 525, 813
SmlI CTYRAG 1 cut(s) 1147
SmoI CTYRAG 1 cut(s) 1147
Sse9I AATT 4 cut(s) 187, 297, 469, 1002
SsiI CCGC 6 cut(s) 14, 502, 912, 1144, 1277, 1286
SspI AATATT 2 cut(s) 606, 787
SspMI CTAG 1 cut(s) 1290
StyD4I CCNGG 2 cut(s) 153, 304
TaaI ACNGT 4 cut(s) 147, 543, 556, 1090
TaiI ACGT 2 cut(s) 712, 1360
TaqI TCGA 4 cut(s) 38, 671, 712, 1148
TasI AATT 4 cut(s) 187, 297, 469, 1002
TatI WGTACW 2 cut(s) 333, 556
TauI GCSGC 1 cut(s) 1289
TfiI GAWTC 6 cut(s) 90, 362, 1012, 1036, 1162, 1227
Tru1I TTAA 3 cut(s) 141, 425, 608
Tru9I TTAA 3 cut(s) 141, 425, 608
TscAI CASTG 5 cut(s) 548, 630, 657, 890, 1260
TseFI GTSAC 1 cut(s) 445
TseI GCWGC 2 cut(s) 452, 620
Tsp45I GTSAC 1 cut(s) 445
TspDTI ATGAA 7 cut(s) 180, 455, 753, 1009, 1044, 1049, 1059
TspRI CASTG 5 cut(s) 548, 630, 657, 890, 1260
VneI GTGCAC 1 cut(s) 816
VpaK11BI GGWCC 1 cut(s) 956
XagI CCTNNNNNAGG 1 cut(s) 1380
XapI RAATTY 1 cut(s) 187
XceI RCATGY 4 cut(s) 407, 530, 1196, 1320
XhoI CTCGAG 1 cut(s) 1147
XmnI GAANNNNTTC 1 cut(s) 300
XspI CTAG 1 cut(s) 1290
Zsp2I ATGCAT 2 cut(s) 61, 1070
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.