MD03G1072300.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003425
Physical Location & Seq
Reverse (-)
5873737 .. 5876352
2616 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1072300.v1.1.491

Sequence Viewer

Length: 1389 bp
ATGACGGAAGTTGGAGAAATATCAGGTGCAAAACCCTGTGAAGAAATCTGGGCCAAGCTTGTTCCGTCAGACTCAAGATACCTGGATGTGGAGATTAGTTCGGATGAGATTGTAGTTTGTTCAGAGATCTTGTTCTCTTCTATTCACAAATGCAAGTGGTGCAAGATAACAAGGAATTCAGACCACTCTTCTGCCACAATACAAAATAAAAGGTCAAATGCAATAGTTGTTGACGGGAGTGTAATCCAAGCTGAAGACACTACTGTTATCAGATGTGGAAGTGAAATTGTTTCAGGTCTTGATAAGGAAGGGTATTTAAGCTACAGGTTCAACGTATTGCCTGGTCCTGAAACTTGTCAGAAGCAGTTAAAGATTTCTATGGATGCTGAGCATGCAAAATGCTGCATTTGTTTGAATATATGGCATGATGTTGTCACCGTTGCTCCTTGCTTCCATAATTTCTGCAATGGTTGCTTCTCGGAGTGGTTAAAAAGATCACAGGAGAAACGTTCAAGTGTACTCTGTCCCCAATGTCGAGCAGTTGTGCAGTTTGTTGGAAGGAATCACTTCCTGCTTAATGTTGCAGAGGAAATGCTAAAAGCAGATTCTTCATTAATACGCTCCAATGATGAAGTGGCAATTTTAGACTCTTATGCAACAATACTATCTAATCTTGTCATAAACTCTGGAAAACATCTACGAGGAAAAAGGGCTCGCTCCCAGGTGGATCAAGAAGCTGATAATGCGTCAAATCCCTGCCCTCAATGTGGTACTGAATATGGTGGGTTTCGCTGCAATCAAACCACCATCCATCTGCAATGTCAAGCATGCGGAGGAATGATGCCTTTGCAAACCAACTACAGTGTACCTCAACACTGTTTAGGATGTGATAAGGCATTCTGTGGTGCATATTGGCATGCGCAAGGGATTGCACAAAGTGATTCTCACCGCATGTGCAGTCGCGAGACTTTTAAACCTATCTCAGAACACACCATCTCTAGAATGCCTACTTCAGCACATGAAAACAATCGACATGAACAAGTTATCACTGAGAAGTGTATTGCACATTTGGGGAGAACATTGCAGGATGTTATTGCAGAATGGCTTGCAAAGTTGGACAACAGAGAAATTGATCGAACAAGGATGCCCCTGAATCATGGTGAGACGATAACTTCTGCAACACCTACATGCAATTACTGTTATGAGAAGCTGATATCATTCCTCTTGTACTGGTTTCGGGTTTCAATTCCCAAATATCATCTGCCCCCAGAAGCGTCAAACAGGGAAAATTGCTGGTATGGGTATGCATGTCGGACACAACACCATAATGATGAGCATGCTCGTAAAAGAAATCATGTATGCCGTCCAACTAGGGGTACTAATATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000209 GO:0000278 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016604 GO:0016605 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031647 GO:0031648 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0036094 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045732 GO:0045786 GO:0045862 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051726 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0097159 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1903047 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_2

Protein Analysis

463

Amino Acids

52.23

Weight (kDa)

7.23

Isoelectric Point (pI)

44.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 133 - 179 4.6e-07 Ring finger domain
zf-C3HC4_2 PF13923 133 - 178 1e-05 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 134 - 178 6.6e-06 Zinc finger, C3HC4 type (RING finger)
zf-CRD PF17979 253 - 416 2.2e-49 Cysteine rich domain with multizinc binding regions
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 921
AccII CGCG 1 cut(s) 963
AciI CCGC 2 cut(s) 831, 949
AclI AACGTT 1 cut(s) 508
AclWI GGATC 1 cut(s) 735
AcsI RAATTY 1 cut(s) 175
AcuI CTGAAG 2 cut(s) 273, 996
AdeI CACNNNGTG 1 cut(s) 938
AfaI GTAC 5 cut(s) 519, 772, 867, 1229, 1378
AfiI CCNNNNNNNGG 3 cut(s) 88, 767, 1373
AgsI TTSAA 4 cut(s) 331, 415, 513, 1245
AjnI CCWGG 3 cut(s) 81, 340, 720
AluBI AGCT 5 cut(s) 58, 251, 321, 737, 1210
AluI AGCT 5 cut(s) 58, 251, 321, 737, 1210
Alw26I GTCTC 2 cut(s) 959, 1157
AlwI GGATC 1 cut(s) 735
AoxI GGCC 1 cut(s) 51
ApeKI GCWGC 2 cut(s) 402, 792
ApoI RAATTY 1 cut(s) 175
AseI ATTAAT 1 cut(s) 614
Asp700I GAANNNNTTC 1 cut(s) 566
AspLEI GCGC 1 cut(s) 922
AspS9I GGNCC 2 cut(s) 51, 344
AsuHPI GGTGA 3 cut(s) 427, 938, 1172
AvaII GGWCC 1 cut(s) 344
BanII GRGCYC 1 cut(s) 715
BbsI GAAGAC 1 cut(s) 261
BbvI GCAGC 2 cut(s) 389, 779
BccI CCATC 3 cut(s) 815, 819, 1001
BceAI ACGGC 1 cut(s) 1347
BciT130I CCWGG 3 cut(s) 83, 342, 722
BcoDI GTCTC 2 cut(s) 959, 1157
BfaI CTAG 2 cut(s) 999, 1371
BfmI CTRYAG 2 cut(s) 322, 859
BglII AGATCT 1 cut(s) 126
BisI GCNGC 2 cut(s) 403, 793
BlpI GCTNAGC 1 cut(s) 387
BlsI GCNGC 2 cut(s) 404, 794
Bme1390I CCNGG 3 cut(s) 83, 342, 722
Bme18I GGWCC 1 cut(s) 344
BmgT120I GGNCC 2 cut(s) 51, 344
BmrFI CCNGG 3 cut(s) 83, 342, 722
BmsI GCATC 3 cut(s) 373, 831, 1134
BpiI GAAGAC 1 cut(s) 261
Bpu1102I GCTNAGC 1 cut(s) 387
BpuEI CTTGAG 1 cut(s) 58
BsaJI CCNNGG 1 cut(s) 720
BsaXI ACNNNNNCTCC 2 cut(s) 427, 457
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 767, 1373
Bse1I ACTGG 1 cut(s) 1235
Bse3DI GCAATG 3 cut(s) 472, 824, 1079
BseBI CCWGG 3 cut(s) 83, 342, 722
BseDI CCNNGG 1 cut(s) 720
BseGI GGATG 7 cut(s) 91, 109, 388, 807, 890, 1093, 1149
BseLI CCNNNNNNNGG 3 cut(s) 88, 767, 1373
BseMI GCAATG 3 cut(s) 472, 824, 1079
BseMII CTCAG 3 cut(s) 378, 996, 1041
BseNI ACTGG 1 cut(s) 1235
BseXI GCAGC 2 cut(s) 389, 779
BsgI GTGCAG 2 cut(s) 566, 976
Bsh1236I CGCG 1 cut(s) 963
BshFI GGCC 1 cut(s) 53
BslFI GGGAC 1 cut(s) 510
BslI CCNNNNNNNGG 3 cut(s) 88, 767, 1373
BsmAI GTCTC 2 cut(s) 959, 1157
BsmBI CGTCTC 1 cut(s) 1157
BsmFI GGGAC 1 cut(s) 510
BsmI GAATGC 2 cut(s) 896, 1008
BsnI GGCC 1 cut(s) 53
Bsp1286I GDGCHC 1 cut(s) 715
Bsp143I GATC 4 cut(s) 126, 494, 727, 1132
Bsp1720I GCTNAGC 1 cut(s) 387
Bsp68I TCGCGA 1 cut(s) 963
BspACI CCGC 2 cut(s) 831, 949
BspANI GGCC 1 cut(s) 53
BspCNI CTCAG 3 cut(s) 379, 995, 1042
BspFNI CGCG 1 cut(s) 963
BspPI GGATC 1 cut(s) 735
BsrDI GCAATG 3 cut(s) 472, 824, 1079
BsrI ACTGG 1 cut(s) 1235
BssECI CCNNGG 1 cut(s) 720
BssMI GATC 4 cut(s) 126, 494, 727, 1132
Bst2UI CCWGG 3 cut(s) 83, 342, 722
Bst4CI ACNGT 5 cut(s) 265, 439, 863, 878, 1199
Bst6I CTCTTC 2 cut(s) 142, 193
BstAPI GCANNNNNTGC 2 cut(s) 159, 471
BstC8I GCNNGC 6 cut(s) 393, 715, 829, 918, 1107, 1338
BstDEI CTNAG 3 cut(s) 387, 982, 1050
BstF5I GGATG 7 cut(s) 91, 109, 388, 807, 890, 1093, 1149
BstFNI CGCG 1 cut(s) 963
BstHHI GCGC 1 cut(s) 922
BstKTI GATC 4 cut(s) 129, 497, 730, 1135
BstMAI GTCTC 2 cut(s) 959, 1157
BstMBI GATC 4 cut(s) 126, 494, 727, 1132
BstMWI GCNNNNNNNGC 4 cut(s) 159, 392, 471, 743
BstNI CCWGG 3 cut(s) 83, 342, 722
BstNSI RCATGY 7 cut(s) 395, 831, 920, 955, 1191, 1311, 1340
BstSCI CCNGG 3 cut(s) 81, 340, 720
BstSFI CTRYAG 2 cut(s) 322, 859
BstUI CGCG 1 cut(s) 963
BstV1I GCAGC 2 cut(s) 389, 779
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
BsuRI GGCC 1 cut(s) 53
BtsCI GGATG 7 cut(s) 91, 109, 388, 807, 890, 1093, 1149
BtsIMutI CAGTG 3 cut(s) 868, 874, 1047
BtuMI TCGCGA 1 cut(s) 963
Cac8I GCNNGC 6 cut(s) 393, 715, 829, 918, 1107, 1338
CfoI GCGC 1 cut(s) 922
Cfr13I GGNCC 2 cut(s) 51, 344
CseI GACGC 2 cut(s) 735, 1263
Csp6I GTAC 5 cut(s) 518, 771, 866, 1228, 1377
CviJI RGCY 8 cut(s) 53, 58, 251, 321, 713, 737, 1105, 1210
CviKI_1 RGCY 8 cut(s) 53, 58, 251, 321, 713, 737, 1105, 1210
CviQI GTAC 5 cut(s) 518, 771, 866, 1228, 1377
DdeI CTNAG 3 cut(s) 387, 982, 1050
DpnI GATC 4 cut(s) 128, 496, 729, 1134
DpnII GATC 4 cut(s) 126, 494, 727, 1132
DraI TTTAAA 1 cut(s) 973
DraIII CACNNNGTG 1 cut(s) 938
Eam1104I CTCTTC 2 cut(s) 142, 193
EarI CTCTTC 2 cut(s) 142, 193
Eco24I GRGCYC 1 cut(s) 715
Eco32I GATATC 1 cut(s) 1215
Eco47I GGWCC 1 cut(s) 344
Eco57I CTGAAG 2 cut(s) 273, 996
EcoRI GAATTC 1 cut(s) 175
EcoRII CCWGG 3 cut(s) 81, 340, 720
EcoRV GATATC 1 cut(s) 1215
EcoT22I ATGCAT 1 cut(s) 1309
EcoT38I GRGCYC 1 cut(s) 715
Esp3I CGTCTC 1 cut(s) 1157
FaqI GGGAC 1 cut(s) 510
Fnu4HI GCNGC 2 cut(s) 403, 793
FokI GGATG 7 cut(s) 98, 116, 395, 794, 897, 1100, 1156
FriOI GRGCYC 1 cut(s) 715
Fsp4HI GCNGC 2 cut(s) 403, 793
FspBI CTAG 2 cut(s) 999, 1371
FspI TGCGCA 1 cut(s) 921
GlaI GCGC 1 cut(s) 921
GluI GCNGC 2 cut(s) 403, 793
HaeIII GGCC 1 cut(s) 53
HgaI GACGC 2 cut(s) 735, 1263
HhaI GCGC 1 cut(s) 922
Hin6I GCGC 1 cut(s) 920
HinP1I GCGC 1 cut(s) 920
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 6 cut(s) 71, 562, 605, 647, 941, 1153
HphI GGTGA 3 cut(s) 427, 938, 1172
Hpy166II GTNNAC 3 cut(s) 232, 518, 866
Hpy188I TCNGA 9 cut(s) 70, 103, 124, 181, 272, 360, 481, 985, 1314
Hpy188III TCNNGA 7 cut(s) 75, 299, 347, 687, 731, 962, 999
Hpy8I GTNNAC 3 cut(s) 232, 518, 866
HpyAV CCTTC 2 cut(s) 302, 552
HpyCH4III ACNGT 5 cut(s) 265, 439, 863, 878, 1199
HpyCH4IV ACGT 2 cut(s) 333, 508
HpyF10VI GCNNNNNNNGC 4 cut(s) 159, 392, 471, 743
HpyF3I CTNAG 3 cut(s) 387, 982, 1050
HpySE526I ACGT 2 cut(s) 333, 508
HspAI GCGC 1 cut(s) 920
Kzo9I GATC 4 cut(s) 126, 494, 727, 1132
LmnI GCTCC 3 cut(s) 448, 626, 722
Lsp1109I GCAGC 2 cut(s) 389, 779
LweI GCATC 3 cut(s) 373, 831, 1134
MaeI CTAG 2 cut(s) 999, 1371
MaeII ACGT 2 cut(s) 333, 508
MaeIII GTNAC 1 cut(s) 433
MalI GATC 4 cut(s) 128, 496, 729, 1134
MboI GATC 4 cut(s) 126, 494, 727, 1132
MboII GAAGA 5 cut(s) 53, 129, 180, 266, 600
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 1 cut(s) 715
MluCI AATT 8 cut(s) 175, 285, 457, 639, 1128, 1192, 1245, 1288
MlyI GAGTC 2 cut(s) 65, 641
MmeI TCCRAC 3 cut(s) 535, 1095, 1292
MnlI CCTC 6 cut(s) 580, 695, 771, 827, 879, 1232
Mph1103I ATGCAT 1 cut(s) 1309
MroXI GAANNNNTTC 1 cut(s) 566
MseI TTAA 6 cut(s) 317, 368, 488, 576, 614, 972
MslI CAYNNNNRTG 3 cut(s) 1186, 1326, 1329
MspR9I CCNGG 3 cut(s) 83, 342, 722
Mva1269I GAATGC 2 cut(s) 896, 1008
MvaI CCWGG 3 cut(s) 83, 342, 722
MvnI CGCG 1 cut(s) 963
MwoI GCNNNNNNNGC 4 cut(s) 159, 392, 471, 743
NdeII GATC 4 cut(s) 126, 494, 727, 1132
NmuCI GTSAC 1 cut(s) 433
NruI TCGCGA 1 cut(s) 963
NsbI TGCGCA 1 cut(s) 921
NsiI ATGCAT 1 cut(s) 1309
NspI RCATGY 7 cut(s) 395, 831, 920, 955, 1191, 1311, 1340
PaeI GCATGC 4 cut(s) 395, 831, 920, 1340
PctI GAATGC 2 cut(s) 896, 1008
PdmI GAANNNNTTC 1 cut(s) 566
PfeI GAWTC 4 cut(s) 562, 605, 941, 1153
PkrI GCNGC 2 cut(s) 404, 794
PleI GAGTC 2 cut(s) 65, 641
PpsI GAGTC 2 cut(s) 65, 641
PshBI ATTAAT 1 cut(s) 614
Psp1406I AACGTT 1 cut(s) 508
Psp6I CCWGG 3 cut(s) 81, 340, 720
PspGI CCWGG 3 cut(s) 81, 340, 720
PspPI GGNCC 2 cut(s) 51, 344
PsuI RGATCY 1 cut(s) 126
RruI TCGCGA 1 cut(s) 963
RsaI GTAC 5 cut(s) 519, 772, 867, 1229, 1378
RsaNI GTAC 5 cut(s) 518, 771, 866, 1228, 1377
RseI CAYNNNNRTG 3 cut(s) 1186, 1326, 1329
SaqAI TTAA 6 cut(s) 317, 368, 488, 576, 614, 972
SatI GCNGC 2 cut(s) 403, 793
Sau3AI GATC 4 cut(s) 126, 494, 727, 1132
Sau96I GGNCC 2 cut(s) 51, 344
SchI GAGTC 2 cut(s) 65, 641
ScrFI CCNGG 3 cut(s) 83, 342, 722
SduI GDGCHC 1 cut(s) 715
SfaNI GCATC 3 cut(s) 373, 831, 1134
SfcI CTRYAG 2 cut(s) 322, 859
SinI GGWCC 1 cut(s) 344
SmiMI CAYNNNNRTG 3 cut(s) 1186, 1326, 1329
SmlI CTYRAG 1 cut(s) 73
SmoI CTYRAG 1 cut(s) 73
SphI GCATGC 4 cut(s) 395, 831, 920, 1340
Sse9I AATT 8 cut(s) 175, 285, 457, 639, 1128, 1192, 1245, 1288
SsiI CCGC 2 cut(s) 831, 949
SspMI CTAG 2 cut(s) 999, 1371
StyD4I CCNGG 3 cut(s) 81, 340, 720
TaaI ACNGT 5 cut(s) 265, 439, 863, 878, 1199
TaiI ACGT 2 cut(s) 336, 511
TaqI TCGA 3 cut(s) 535, 1030, 1135
TasI AATT 8 cut(s) 175, 285, 457, 639, 1128, 1192, 1245, 1288
TatI WGTACW 2 cut(s) 517, 1227
TfiI GAWTC 4 cut(s) 562, 605, 941, 1153
Tru1I TTAA 6 cut(s) 317, 368, 488, 576, 614, 972
Tru9I TTAA 6 cut(s) 317, 368, 488, 576, 614, 972
TscAI CASTG 3 cut(s) 868, 881, 1054
TseFI GTSAC 1 cut(s) 433
TseI GCWGC 2 cut(s) 402, 792
Tsp45I GTSAC 1 cut(s) 433
TspDTI ATGAA 4 cut(s) 600, 645, 1035, 1050
TspGWI ACGGA 2 cut(s) 20, 54
TspRI CASTG 3 cut(s) 868, 881, 1054
VpaK11BI GGWCC 1 cut(s) 344
VspI ATTAAT 1 cut(s) 614
XapI RAATTY 1 cut(s) 175
XbaI TCTAGA 1 cut(s) 998
XceI RCATGY 7 cut(s) 395, 831, 920, 955, 1191, 1311, 1340
XcmI CCANNNNNNNNNTGG 1 cut(s) 631
XmnI GAANNNNTTC 1 cut(s) 566
XspI CTAG 2 cut(s) 999, 1371
Zsp2I ATGCAT 1 cut(s) 1309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.