Rh2DG546100

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003425
Physical Location & Seq
Reverse (-)
77495040 .. 77497548
2509 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG546100.1

Sequence Viewer

Length: 1425 bp
ATGGCAGAAATTGGAGAAAGCTCTGGTGCAAAGTCCTCTGGTGAAATCTGGGCCAAGCTTGTTCCTTCCGACTCGAGATTTCCGGATGTTGAGATCAGAGATCTTGTTCTGACTCAAGATACCCCTCAGAGTCAAGATATTGGTTCAGATGAGAGTTTAGTTTGCTCTCCCCTATTGACAAATGTGAGTGGTGCAGAATTACGAGGAACCCCCGATCAGTCTTCTGCCACTATACAAAATAAGAGTTCAAATGCAATACTTGTTGATGGAGCTGTGATCCAATCCGAAGAGAGTACTGTTATCAGATCTGGAAGTGAAATTATTACAGGTCTTGAGAGAGAAGGGTGTTTAAGCTACAGATTCAATGTGTTGCTTGGTCCTGAAACTTGTCAGAAGATGCTGAAGATTAGTGTGGATGCTGAGCATGCAAAGTGCTGCATTTGTTTAAATATATGGCATGATGCTGTTACCATTGCTCCTTGCTTCCATAACTTCTGCAATGGGTGCTTCTCAGAGTGGTTAAGGAGATCACAGGAGAAACGTTCAAGTGTACTCTGTCCCCACTGTCGAGCAGTTGTGCAATTTGTTGGAAGAAACCACTTCCTGCATAACATTGCCGATGAAATACTGAATGCGGATTCTTCACTGAGACGCTCCAACGAAGACACTGCAGTTTTAGATTCCTATGCAACAGTACGATCAAATCTGGTAATAAAGTCGGGAAAGGGGCTGCTTGGGAAAAGGGTTCGTTCAGCAGTGCAAGATGAAGCTGATAGTGTAGAGCTTCCCTGCCCTCAATGCAGTAATCAATTTGGTGGGTTTCTCTGCAACCGAAGCACTACCCATCTGCAATGTCAAGCATGTGGAGGAATGATGCCTTCACGAACCAATACAAGTGTACCTCAACACTGTATAGGGTGTGATAGGTCATTTTGCGGTGCATATTGGCATGCTCAAGGCGTTGCACAAAGTGCTTCTCACCACATATGCAGTCGCGATACTTTCAAACCAATCACAGAACGCACCATCTCTAGAATTCCTACTTCAGCACATGAAAACAATCGCCATGAACAAGAAATCACTATGAGGTGTATTGCACAAATGGGAAGAACATTGCAGGATGTTATTGCAGAATGGATGGTAATGTTCAATAACAGAACAATTGATCAAACTAACAGGATGCCACTGAAGCATGCCGAGCTGATAACTTCTGGGACATATACATGCAGTGATTGTTATGAGAAACTGATCGCATTCCTCTTGTACTGGTTTCGGGTTTCAGTTTCTGCATCTCATCTTCCCCCAGATGCATCAAAAAGGGAAAATTGCTGGTATGGATATGCATGTCGAACACAGCACCATAATGAAGACCATGCTCGTAAAAGGAATCATGTATGCCGGCCGACTAGGGGTGCTCATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000209 GO:0000278 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016604 GO:0016605 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031647 GO:0031648 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0036094 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045732 GO:0045786 GO:0045862 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051726 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0097159 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1903047 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_2

Protein Analysis

474

Amino Acids

52.74

Weight (kDa)

6.84

Isoelectric Point (pI)

54.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 144 - 190 5e-07 Ring finger domain
zf-C3HC4_2 PF13923 144 - 189 3.8e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 145 - 189 6.2e-06 Zinc finger, C3HC4 type (RING finger)
zf-CRD PF17979 264 - 427 1.3e-47 Cysteine rich domain with multizinc binding regions
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 996
AccIII TCCGGA 1 cut(s) 82
AciI CCGC 2 cut(s) 635, 936
AclI AACGTT 1 cut(s) 541
AclWI GGATC 1 cut(s) 271
AcoI YGGCCR 1 cut(s) 1400
AcsI RAATTY 1 cut(s) 1035
AcuI CTGAAG 3 cut(s) 422, 1029, 1208
AdeI CACNNNGTG 1 cut(s) 971
AfaI GTAC 5 cut(s) 295, 552, 696, 900, 1265
AfiI CCNNNNNNNGG 1 cut(s) 1409
AgsI TTSAA 5 cut(s) 249, 364, 546, 1006, 1150
AluBI AGCT 7 cut(s) 21, 58, 272, 354, 770, 784, 1201
AluI AGCT 7 cut(s) 21, 58, 272, 354, 770, 784, 1201
Alw21I GWGCWC 1 cut(s) 1417
Alw26I GTCTC 1 cut(s) 643
AlwI GGATC 1 cut(s) 271
AlwNI CAGNNNCTG 1 cut(s) 1286
Ama87I CYCGRG 1 cut(s) 73
Aor13HI TCCGGA 1 cut(s) 82
AoxI GGCC 2 cut(s) 51, 1400
ApeKI GCWGC 2 cut(s) 435, 730
ApoI RAATTY 1 cut(s) 1035
AspS9I GGNCC 2 cut(s) 51, 377
AsuHPI GGTGA 2 cut(s) 53, 971
AvaI CYCGRG 1 cut(s) 73
AvaII GGWCC 1 cut(s) 377
BaeI ACNNNNGTAYC 2 cut(s) 882, 915
BbsI GAAGAC 3 cut(s) 213, 669, 1374
Bbv12I GWGCWC 1 cut(s) 1417
BbvI GCAGC 2 cut(s) 422, 717
BccI CCATC 4 cut(s) 260, 852, 1034, 1132
BcgI CGANNNNNNTGC 2 cut(s) 650, 684
BclI TGATCA 1 cut(s) 1165
BcoDI GTCTC 1 cut(s) 643
BfaI CTAG 2 cut(s) 1032, 1407
BfmI CTRYAG 2 cut(s) 355, 669
BglII AGATCT 2 cut(s) 100, 305
BisI GCNGC 2 cut(s) 436, 731
BlpI GCTNAGC 1 cut(s) 420
BlsI GCNGC 2 cut(s) 437, 732
BmcAI AGTACT 1 cut(s) 295
Bme18I GGWCC 1 cut(s) 377
BmeT110I CYCGRG 1 cut(s) 73
BmgT120I GGNCC 2 cut(s) 51, 377
BmiI GGNNCC 1 cut(s) 208
BmsI GCATC 8 cut(s) 387, 406, 451, 864, 1170, 1297, 1298, 1319
BpiI GAAGAC 3 cut(s) 213, 669, 1374
Bpu1102I GCTNAGC 1 cut(s) 420
BpuEI CTTGAG 3 cut(s) 99, 353, 939
BsaWI WCCGGW 1 cut(s) 82
BsaXI ACNNNNNCTCC 2 cut(s) 460, 490
Bsc4I CCNNNNNNNGG 1 cut(s) 1409
Bse118I RCCGGY 1 cut(s) 1398
Bse1I ACTGG 1 cut(s) 1271
Bse3DI GCAATG 5 cut(s) 471, 505, 612, 857, 1112
BseAI TCCGGA 1 cut(s) 82
BseGI GGATG 5 cut(s) 91, 421, 1126, 1143, 1185
BseLI CCNNNNNNNGG 1 cut(s) 1409
BseMI GCAATG 5 cut(s) 471, 505, 612, 857, 1112
BseMII CTCAG 4 cut(s) 140, 411, 525, 638
BseNI ACTGG 1 cut(s) 1271
BseX3I CGGCCG 1 cut(s) 1400
BseXI GCAGC 2 cut(s) 422, 717
BsgI GTGCAG 1 cut(s) 213
Bsh1236I CGCG 1 cut(s) 996
Bsh1285I CGRYCG 1 cut(s) 1403
BshFI GGCC 2 cut(s) 53, 1402
BsiEI CGRYCG 1 cut(s) 1403
BsiHKAI GWGCWC 1 cut(s) 1417
BsiHKCI CYCGRG 1 cut(s) 73
BsiSI CCGG 2 cut(s) 83, 1399
BslFI GGGAC 2 cut(s) 543, 1228
BslI CCNNNNNNNGG 1 cut(s) 1409
BsmAI GTCTC 1 cut(s) 643
BsmBI CGTCTC 1 cut(s) 643
BsmFI GGGAC 2 cut(s) 543, 1228
BsmI GAATGC 2 cut(s) 637, 1253
BsnI GGCC 2 cut(s) 53, 1402
BsoBI CYCGRG 1 cut(s) 73
Bsp1286I GDGCHC 1 cut(s) 1417
Bsp13I TCCGGA 1 cut(s) 82
Bsp143I GATC 9 cut(s) 93, 100, 214, 276, 305, 527, 698, 1165, 1248
Bsp1720I GCTNAGC 1 cut(s) 420
Bsp68I TCGCGA 1 cut(s) 996
BspACI CCGC 2 cut(s) 635, 936
BspANI GGCC 2 cut(s) 53, 1402
BspCNI CTCAG 4 cut(s) 139, 412, 524, 639
BspEI TCCGGA 1 cut(s) 82
BspFNI CGCG 1 cut(s) 996
BspLI GGNNCC 1 cut(s) 208
BspMAI CTGCAG 1 cut(s) 673
BspPI GGATC 1 cut(s) 271
BsrDI GCAATG 5 cut(s) 471, 505, 612, 857, 1112
BsrFI RCCGGY 1 cut(s) 1398
BsrI ACTGG 1 cut(s) 1271
BssAI RCCGGY 1 cut(s) 1398
BssMI GATC 9 cut(s) 93, 100, 214, 276, 305, 527, 698, 1165, 1248
Bst4CI ACNGT 4 cut(s) 298, 566, 694, 911
Bst6I CTCTTC 1 cut(s) 282
BstAPI GCANNNNNTGC 2 cut(s) 504, 971
BstC8I GCNNGC 4 cut(s) 426, 951, 1194, 1400
BstDEI CTNAG 4 cut(s) 126, 420, 511, 647
BstF5I GGATG 5 cut(s) 91, 421, 1126, 1143, 1185
BstFNI CGCG 1 cut(s) 996
BstKTI GATC 9 cut(s) 96, 103, 217, 279, 308, 530, 701, 1168, 1251
BstMAI GTCTC 1 cut(s) 643
BstMBI GATC 9 cut(s) 93, 100, 214, 276, 305, 527, 698, 1165, 1248
BstMCI CGRYCG 1 cut(s) 1403
BstMWI GCNNNNNNNGC 7 cut(s) 425, 504, 798, 834, 971, 1189, 1198
BstNSI RCATGY 6 cut(s) 428, 864, 953, 1196, 1227, 1347
BstSFI CTRYAG 2 cut(s) 355, 669
BstUI CGCG 1 cut(s) 996
BstV1I GCAGC 2 cut(s) 422, 717
BstV2I GAAGAC 3 cut(s) 213, 669, 1374
BstX2I RGATCY 2 cut(s) 100, 305
BstYI RGATCY 2 cut(s) 100, 305
BstZI CGGCCG 1 cut(s) 1400
BsuRI GGCC 2 cut(s) 53, 1402
BtsCI GGATG 5 cut(s) 91, 421, 1126, 1143, 1185
BtsI GCAGTG 3 cut(s) 666, 762, 1234
BtsIMutI CAGTG 7 cut(s) 562, 644, 666, 762, 907, 1184, 1234
BtuMI TCGCGA 1 cut(s) 996
Cac8I GCNNGC 4 cut(s) 426, 951, 1194, 1400
CaiI CAGNNNCTG 1 cut(s) 1286
Cfr10I RCCGGY 1 cut(s) 1398
Cfr13I GGNCC 2 cut(s) 51, 377
CseI GACGC 1 cut(s) 660
Csp6I GTAC 5 cut(s) 294, 551, 695, 899, 1264
CviQI GTAC 5 cut(s) 294, 551, 695, 899, 1264
DdeI CTNAG 4 cut(s) 126, 420, 511, 647
DpnI GATC 9 cut(s) 95, 102, 216, 278, 307, 529, 700, 1167, 1250
DpnII GATC 9 cut(s) 93, 100, 214, 276, 305, 527, 698, 1165, 1248
DraI TTTAAA 1 cut(s) 447
DraIII CACNNNGTG 1 cut(s) 971
EaeI YGGCCR 1 cut(s) 1400
EagI CGGCCG 1 cut(s) 1400
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
EclXI CGGCCG 1 cut(s) 1400
Eco47I GGWCC 1 cut(s) 377
Eco52I CGGCCG 1 cut(s) 1400
Eco57I CTGAAG 3 cut(s) 422, 1029, 1208
Eco88I CYCGRG 1 cut(s) 73
EcoRI GAATTC 1 cut(s) 1035
EcoT22I ATGCAT 2 cut(s) 1312, 1345
Esp3I CGTCTC 1 cut(s) 643
FaqI GGGAC 2 cut(s) 543, 1228
FauNDI CATATG 1 cut(s) 986
FbaI TGATCA 1 cut(s) 1165
Fnu4HI GCNGC 2 cut(s) 436, 731
FokI GGATG 5 cut(s) 98, 428, 1133, 1150, 1192
Fsp4HI GCNGC 2 cut(s) 436, 731
FspBI CTAG 2 cut(s) 1032, 1407
GluI GCNGC 2 cut(s) 436, 731
HaeIII GGCC 2 cut(s) 53, 1402
HapII CCGG 2 cut(s) 83, 1399
HgaI GACGC 1 cut(s) 660
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 7 cut(s) 71, 112, 130, 360, 638, 680, 1387
HpaII CCGG 2 cut(s) 83, 1399
HphI GGTGA 2 cut(s) 53, 971
Hpy166II GTNNAC 2 cut(s) 551, 899
Hpy188I TCNGA 9 cut(s) 70, 98, 111, 129, 148, 286, 305, 393, 514
Hpy8I GTNNAC 2 cut(s) 551, 899
HpyAV CCTTC 3 cut(s) 75, 335, 888
HpyCH4III ACNGT 4 cut(s) 298, 566, 694, 911
HpyCH4IV ACGT 1 cut(s) 541
HpyF10VI GCNNNNNNNGC 7 cut(s) 425, 504, 798, 834, 971, 1189, 1198
HpyF3I CTNAG 4 cut(s) 126, 420, 511, 647
HpySE526I ACGT 1 cut(s) 541
Kpn2I TCCGGA 1 cut(s) 82
KroI GCCGGC 1 cut(s) 1398
KroNI GCCGGC 1 cut(s) 1400
Ksp22I TGATCA 1 cut(s) 1165
Kzo9I GATC 9 cut(s) 93, 100, 214, 276, 305, 527, 698, 1165, 1248
LmnI GCTCC 3 cut(s) 269, 481, 659
Lsp1109I GCAGC 2 cut(s) 422, 717
LweI GCATC 8 cut(s) 387, 406, 451, 864, 1170, 1297, 1298, 1319
MaeI CTAG 2 cut(s) 1032, 1407
MaeII ACGT 1 cut(s) 541
MaeIII GTNAC 1 cut(s) 466
MalI GATC 9 cut(s) 95, 102, 216, 278, 307, 529, 700, 1167, 1250
MboI GATC 9 cut(s) 93, 100, 214, 276, 305, 527, 698, 1165, 1248
MfeI CAATTG 1 cut(s) 1161
MflI RGATCY 2 cut(s) 100, 305
MhlI GDGCHC 1 cut(s) 1417
MluCI AATT 8 cut(s) 9, 197, 318, 581, 809, 1035, 1161, 1324
MlyI GAGTC 3 cut(s) 65, 106, 139
MmeI TCCRAC 3 cut(s) 93, 568, 681
MnlI CCTC 8 cut(s) 46, 135, 197, 804, 860, 912, 1080, 1268
Mph1103I ATGCAT 2 cut(s) 1312, 1345
MroI TCCGGA 1 cut(s) 82
MroNI GCCGGC 1 cut(s) 1398
MseI TTAA 3 cut(s) 350, 446, 521
MslI CAYNNNNRTG 2 cut(s) 1222, 1362
MspI CCGG 2 cut(s) 83, 1399
MunI CAATTG 1 cut(s) 1161
Mva1269I GAATGC 2 cut(s) 637, 1253
MvnI CGCG 1 cut(s) 996
MwoI GCNNNNNNNGC 7 cut(s) 425, 504, 798, 834, 971, 1189, 1198
NaeI GCCGGC 1 cut(s) 1400
NdeI CATATG 1 cut(s) 986
NdeII GATC 9 cut(s) 93, 100, 214, 276, 305, 527, 698, 1165, 1248
NgoMIV GCCGGC 1 cut(s) 1398
NlaIV GGNNCC 1 cut(s) 208
NmeAIII GCCGAG 1 cut(s) 1222
NruI TCGCGA 1 cut(s) 996
NsiI ATGCAT 2 cut(s) 1312, 1345
NspI RCATGY 6 cut(s) 428, 864, 953, 1196, 1227, 1347
PaeI GCATGC 3 cut(s) 428, 953, 1196
PaeR7I CTCGAG 1 cut(s) 73
PctI GAATGC 2 cut(s) 637, 1253
PdiI GCCGGC 1 cut(s) 1400
PfeI GAWTC 4 cut(s) 360, 638, 680, 1387
PkrI GCNGC 2 cut(s) 437, 732
PleI GAGTC 3 cut(s) 65, 106, 138
PpsI GAGTC 3 cut(s) 65, 106, 138
Psp1406I AACGTT 1 cut(s) 541
PspN4I GGNNCC 1 cut(s) 208
PspPI GGNCC 2 cut(s) 51, 377
PstI CTGCAG 1 cut(s) 673
PstNI CAGNNNCTG 1 cut(s) 1286
PsuI RGATCY 2 cut(s) 100, 305
RruI TCGCGA 1 cut(s) 996
RsaI GTAC 5 cut(s) 295, 552, 696, 900, 1265
RsaNI GTAC 5 cut(s) 294, 551, 695, 899, 1264
RseI CAYNNNNRTG 2 cut(s) 1222, 1362
SaqAI TTAA 3 cut(s) 350, 446, 521
SatI GCNGC 2 cut(s) 436, 731
Sau3AI GATC 9 cut(s) 93, 100, 214, 276, 305, 527, 698, 1165, 1248
Sau96I GGNCC 2 cut(s) 51, 377
ScaI AGTACT 1 cut(s) 295
SchI GAGTC 3 cut(s) 65, 106, 139
SduI GDGCHC 1 cut(s) 1417
SfaNI GCATC 8 cut(s) 387, 406, 451, 864, 1170, 1297, 1298, 1319
SfcI CTRYAG 2 cut(s) 355, 669
Sfr274I CTCGAG 1 cut(s) 73
SinI GGWCC 1 cut(s) 377
SlaI CTCGAG 1 cut(s) 73
SmiMI CAYNNNNRTG 2 cut(s) 1222, 1362
SmlI CTYRAG 4 cut(s) 73, 114, 332, 954
SmoI CTYRAG 4 cut(s) 73, 114, 332, 954
SphI GCATGC 3 cut(s) 428, 953, 1196
Sse9I AATT 8 cut(s) 9, 197, 318, 581, 809, 1035, 1161, 1324
SsiI CCGC 2 cut(s) 635, 936
SspMI CTAG 2 cut(s) 1032, 1407
TaaI ACNGT 4 cut(s) 298, 566, 694, 911
TaiI ACGT 1 cut(s) 544
TaqI TCGA 3 cut(s) 74, 568, 1348
TasI AATT 8 cut(s) 9, 197, 318, 581, 809, 1035, 1161, 1324
TatI WGTACW 3 cut(s) 293, 550, 1263
TfiI GAWTC 4 cut(s) 360, 638, 680, 1387
Tru1I TTAA 3 cut(s) 350, 446, 521
Tru9I TTAA 3 cut(s) 350, 446, 521
TscAI CASTG 7 cut(s) 569, 651, 673, 762, 914, 1191, 1234
TseI GCWGC 2 cut(s) 435, 730
TspDTI ATGAA 5 cut(s) 636, 780, 1068, 1083, 1380
TspRI CASTG 7 cut(s) 569, 651, 673, 762, 914, 1191, 1234
VpaK11BI GGWCC 1 cut(s) 377
XapI RAATTY 1 cut(s) 1035
XbaI TCTAGA 1 cut(s) 1031
XceI RCATGY 6 cut(s) 428, 864, 953, 1196, 1227, 1347
XhoI CTCGAG 1 cut(s) 73
XspI CTAG 2 cut(s) 1032, 1407
ZrmI AGTACT 1 cut(s) 295
Zsp2I ATGCAT 2 cut(s) 1312, 1345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.