MD11G1078600.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003425
Physical Location & Seq
Reverse (-)
6709493 .. 6710505
1013 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1078600.v1.1.491

Sequence Viewer

Length: 960 bp
ATGGCAGAAGTTGGAGAGAGCTCGGGTGCAAAACCCTGTGAAGAATTCTGGGCCAAGCTCGTTCCATCAGACTCGAGATATCCGGATGTTGAGATTAGTTCACGTGAGATTGTAGTTTGCTCAGAGATCTTGTTCTCTTCTATTCGCAAATGCAAGTGGTGCAAGATTTCTATGGATGCCGAGCATGCAATATGCTGCAATTGCTTGAACATATGGCATGATGTAGTTACCGTTGCTCCTTGCTTCCATAATTTCTGTAATGGATGCTTCTCAGAGTGGTTAAAGAGATCACTGGAGAAACGTTCAAGTGTACTCTGTCCCCATTGTCGAGCAGTTGTACAGTTTGTTGGAAGAAATCACTTCTTGCTTAACGTTGTGGATGAAATACTACAAGCTGATTCTTCACTAAAACGCACCAATGATGAAGGCTCACTCCCAGGTGATGAAGAAGCTGATAATCCATCAAATCCATGCCCTCAATGTGGTACTGAATATGGTGGGTTCTGCTGCAATCAAACCACTATCCATCTGCAATGTCAAGCATGCGGAGGAATGATGCCTTTGCACACCAATAACAGTATACCTCAACACTGTTTAGGATGTGATAAGGAATTTTGCGGTACATATTGGCATGCGCAAGGGGTTTCACAAAGTGATTCTCACCGTGTGTGCAGGCGTGAGACTTTTAAACCAATCTCAGAATGGACCATCTCTGGAATCCCTACTTCAGCTCATGAAAACAATCGACATGAACAAGTTATCACTGAGAAGTGTATTGCACAGTTGGGGAGAACATTACAGAATGTTATTGCAGAATGGCTTGCAAAGTTGAACAACAGAGAAATTGATCGAACGAGGATGCCCCTGAATCCTGCTGAAACAATAACTTCTGGGACACCTACGTGCAATTACTGTTACGAGAAGCTAATCTCCTTCCTCTTGTACTGGTTTCGGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000209 GO:0000278 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016604 GO:0016605 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031647 GO:0031648 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0036094 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045732 GO:0045786 GO:0045862 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051726 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0097159 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1903047 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_2

Protein Analysis

320

Amino Acids

36.04

Weight (kDa)

5.95

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CRD PF17979 158 - 319 4.9e-47 Cysteine rich domain with multizinc binding regions
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 636
AccI GTMKAC 1 cut(s) 580
AccIII TCCGGA 1 cut(s) 82
AciI CCGC 2 cut(s) 546, 618
AclI AACGTT 2 cut(s) 301, 372
AcsI RAATTY 2 cut(s) 44, 611
AcuI CTGAAG 1 cut(s) 711
AcvI CACGTG 1 cut(s) 104
AdeI CACNNNGTG 2 cut(s) 653, 667
AfaI GTAC 5 cut(s) 312, 339, 487, 622, 944
AfiI CCNNNNNNNGG 1 cut(s) 482
AgsI TTSAA 3 cut(s) 208, 306, 832
AjnI CCWGG 1 cut(s) 436
AleI CACNNNNGTG 1 cut(s) 901
AluBI AGCT 6 cut(s) 21, 58, 395, 452, 731, 925
AluI AGCT 6 cut(s) 21, 58, 395, 452, 731, 925
Alw21I GWGCWC 1 cut(s) 23
Alw26I GTCTC 1 cut(s) 674
Ama87I CYCGRG 2 cut(s) 22, 73
Aor13HI TCCGGA 1 cut(s) 82
AoxI GGCC 1 cut(s) 51
ApeKI GCWGC 2 cut(s) 195, 507
ApoI RAATTY 2 cut(s) 44, 611
AspLEI GCGC 1 cut(s) 637
AspS9I GGNCC 2 cut(s) 51, 705
AsuHPI GGTGA 2 cut(s) 452, 653
AvaI CYCGRG 2 cut(s) 22, 73
AvaII GGWCC 1 cut(s) 705
BanII GRGCYC 1 cut(s) 23
BbrPI CACGTG 1 cut(s) 104
Bbv12I GWGCWC 1 cut(s) 23
BbvI GCAGC 2 cut(s) 182, 494
BccI CCATC 4 cut(s) 73, 469, 534, 716
BciT130I CCWGG 1 cut(s) 438
BcoDI GTCTC 1 cut(s) 674
BglII AGATCT 1 cut(s) 126
BisI GCNGC 2 cut(s) 196, 508
BlsI GCNGC 2 cut(s) 197, 509
Bme1390I CCNGG 1 cut(s) 438
Bme18I GGWCC 1 cut(s) 705
BmeT110I CYCGRG 2 cut(s) 22, 73
BmgT120I GGNCC 2 cut(s) 51, 705
BmrFI CCNGG 1 cut(s) 438
BmsI GCATC 4 cut(s) 166, 254, 546, 849
BpmI CTGGAG 1 cut(s) 314
BsaAI YACGTR 2 cut(s) 104, 903
BsaJI CCNNGG 1 cut(s) 436
BsaWI WCCGGW 1 cut(s) 82
BsaXI ACNNNNNCTCC 2 cut(s) 220, 250
Bsc4I CCNNNNNNNGG 1 cut(s) 482
Bse1I ACTGG 2 cut(s) 297, 950
Bse3DI GCAATG 1 cut(s) 539
BseAI TCCGGA 1 cut(s) 82
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 1 cut(s) 436
BseGI GGATG 6 cut(s) 91, 181, 269, 385, 605, 864
BseLI CCNNNNNNNGG 1 cut(s) 482
BseMI GCAATG 1 cut(s) 539
BseMII CTCAG 4 cut(s) 135, 285, 711, 756
BseNI ACTGG 2 cut(s) 297, 950
BseXI GCAGC 2 cut(s) 182, 494
BsgI GTGCAG 1 cut(s) 691
BshFI GGCC 1 cut(s) 53
BsiHKAI GWGCWC 1 cut(s) 23
BsiHKCI CYCGRG 2 cut(s) 22, 73
BsiSI CCGG 1 cut(s) 83
BslFI GGGAC 2 cut(s) 303, 907
BslI CCNNNNNNNGG 1 cut(s) 482
BsmAI GTCTC 1 cut(s) 674
BsmFI GGGAC 2 cut(s) 303, 907
BsnI GGCC 1 cut(s) 53
BsoBI CYCGRG 2 cut(s) 22, 73
Bsp1286I GDGCHC 1 cut(s) 23
Bsp13I TCCGGA 1 cut(s) 82
Bsp1407I TGTACA 1 cut(s) 337
Bsp143I GATC 3 cut(s) 126, 287, 847
BspACI CCGC 2 cut(s) 546, 618
BspANI GGCC 1 cut(s) 53
BspCNI CTCAG 4 cut(s) 134, 284, 710, 757
BspEI TCCGGA 1 cut(s) 82
BspHI TCATGA 1 cut(s) 733
BsrDI GCAATG 1 cut(s) 539
BsrGI TGTACA 1 cut(s) 337
BsrI ACTGG 2 cut(s) 297, 950
BssECI CCNNGG 1 cut(s) 436
BssMI GATC 3 cut(s) 126, 287, 847
BssNAI GTATAC 1 cut(s) 581
Bst1107I GTATAC 1 cut(s) 581
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 7 cut(s) 232, 342, 578, 593, 665, 783, 914
Bst6I CTCTTC 1 cut(s) 142
BstAPI GCANNNNNTGC 1 cut(s) 159
BstAUI TGTACA 1 cut(s) 337
BstBAI YACGTR 2 cut(s) 104, 903
BstC8I GCNNGC 5 cut(s) 186, 544, 633, 674, 822
BstDEI CTNAG 4 cut(s) 121, 271, 697, 765
BstF5I GGATG 6 cut(s) 91, 181, 269, 385, 605, 864
BstHHI GCGC 1 cut(s) 637
BstKTI GATC 3 cut(s) 129, 290, 850
BstMAI GTCTC 1 cut(s) 674
BstMBI GATC 3 cut(s) 126, 287, 847
BstMWI GCNNNNNNNGC 3 cut(s) 159, 185, 201
BstNI CCWGG 1 cut(s) 438
BstNSI RCATGY 3 cut(s) 188, 546, 635
BstSCI CCNGG 1 cut(s) 436
BstV1I GCAGC 2 cut(s) 182, 494
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
BstZ17I GTATAC 1 cut(s) 581
BsuRI GGCC 1 cut(s) 53
BtsCI GGATG 6 cut(s) 91, 181, 269, 385, 605, 864
BtsIMutI CAGTG 3 cut(s) 290, 589, 762
Cac8I GCNNGC 5 cut(s) 186, 544, 633, 674, 822
CciI TCATGA 1 cut(s) 733
CfoI GCGC 1 cut(s) 637
Cfr13I GGNCC 2 cut(s) 51, 705
Csp6I GTAC 5 cut(s) 311, 338, 486, 621, 943
CviAII CATG 7 cut(s) 185, 218, 471, 543, 632, 734, 749
CviJI RGCY 9 cut(s) 21, 53, 58, 395, 429, 452, 731, 820, 925
CviKI_1 RGCY 9 cut(s) 21, 53, 58, 395, 429, 452, 731, 820, 925
CviQI GTAC 5 cut(s) 311, 338, 486, 621, 943
DdeI CTNAG 4 cut(s) 121, 271, 697, 765
DpnI GATC 3 cut(s) 128, 289, 849
DpnII GATC 3 cut(s) 126, 287, 847
DraI TTTAAA 1 cut(s) 688
DraIII CACNNNGTG 2 cut(s) 653, 667
Eam1104I CTCTTC 1 cut(s) 142
EarI CTCTTC 1 cut(s) 142
Ecl136II GAGCTC 1 cut(s) 21
Eco24I GRGCYC 1 cut(s) 23
Eco32I GATATC 1 cut(s) 80
Eco47I GGWCC 1 cut(s) 705
Eco53kI GAGCTC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 711
Eco72I CACGTG 1 cut(s) 104
Eco88I CYCGRG 2 cut(s) 22, 73
EcoICRI GAGCTC 1 cut(s) 21
EcoRI GAATTC 1 cut(s) 44
EcoRII CCWGG 1 cut(s) 436
EcoRV GATATC 1 cut(s) 80
EcoT38I GRGCYC 1 cut(s) 23
FaeI CATG 7 cut(s) 188, 221, 474, 546, 635, 737, 752
FaqI GGGAC 2 cut(s) 303, 907
FatI CATG 7 cut(s) 184, 217, 470, 542, 631, 733, 748
FauNDI CATATG 1 cut(s) 212
FblI GTMKAC 1 cut(s) 580
Fnu4HI GCNGC 2 cut(s) 196, 508
FokI GGATG 6 cut(s) 98, 188, 276, 392, 612, 871
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 2 cut(s) 196, 508
FspI TGCGCA 1 cut(s) 636
GlaI GCGC 1 cut(s) 636
GluI GCNGC 2 cut(s) 196, 508
GsuI CTGGAG 1 cut(s) 314
HaeIII GGCC 1 cut(s) 53
HapII CCGG 1 cut(s) 83
HhaI GCGC 1 cut(s) 637
Hin1II CATG 7 cut(s) 188, 221, 474, 546, 635, 737, 752
Hin6I GCGC 1 cut(s) 635
HinP1I GCGC 1 cut(s) 635
HinfI GANTC 5 cut(s) 71, 398, 656, 717, 868
HpaII CCGG 1 cut(s) 83
HphI GGTGA 2 cut(s) 452, 653
Hpy166II GTNNAC 3 cut(s) 101, 311, 581
Hpy188I TCNGA 5 cut(s) 70, 124, 274, 700, 954
Hpy188III TCNNGA 4 cut(s) 75, 83, 714, 734
Hpy8I GTNNAC 3 cut(s) 101, 311, 581
HpyAV CCTTC 2 cut(s) 419, 943
HpyCH4III ACNGT 7 cut(s) 232, 342, 578, 593, 665, 783, 914
HpyCH4IV ACGT 4 cut(s) 103, 301, 372, 902
HpyF10VI GCNNNNNNNGC 3 cut(s) 159, 185, 201
HpyF3I CTNAG 4 cut(s) 121, 271, 697, 765
HpySE526I ACGT 4 cut(s) 103, 301, 372, 902
Hsp92II CATG 7 cut(s) 188, 221, 474, 546, 635, 737, 752
HspAI GCGC 1 cut(s) 635
Kpn2I TCCGGA 1 cut(s) 82
Kzo9I GATC 3 cut(s) 126, 287, 847
LmnI GCTCC 1 cut(s) 241
Lsp1109I GCAGC 2 cut(s) 182, 494
LweI GCATC 4 cut(s) 166, 254, 546, 849
MaeII ACGT 4 cut(s) 103, 301, 372, 902
MaeIII GTNAC 2 cut(s) 226, 914
MalI GATC 3 cut(s) 128, 289, 849
MboI GATC 3 cut(s) 126, 287, 847
MboII GAAGA 5 cut(s) 53, 129, 363, 393, 458
MfeI CAATTG 1 cut(s) 199
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 1 cut(s) 23
MluCI AATT 6 cut(s) 44, 199, 250, 611, 843, 907
MlyI GAGTC 1 cut(s) 65
MmeI TCCRAC 1 cut(s) 328
MnlI CCTC 5 cut(s) 486, 542, 594, 849, 947
MroI TCCGGA 1 cut(s) 82
MseI TTAA 3 cut(s) 281, 369, 687
MslI CAYNNNNRTG 1 cut(s) 901
MspI CCGG 1 cut(s) 83
MspR9I CCNGG 1 cut(s) 438
MunI CAATTG 1 cut(s) 199
MvaI CCWGG 1 cut(s) 438
MwoI GCNNNNNNNGC 3 cut(s) 159, 185, 201
NdeI CATATG 1 cut(s) 212
NdeII GATC 3 cut(s) 126, 287, 847
NlaIII CATG 7 cut(s) 188, 221, 474, 546, 635, 737, 752
NmeAIII GCCGAG 1 cut(s) 205
NsbI TGCGCA 1 cut(s) 636
NspI RCATGY 3 cut(s) 188, 546, 635
OliI CACNNNNGTG 1 cut(s) 901
PaeI GCATGC 3 cut(s) 188, 546, 635
PaeR7I CTCGAG 1 cut(s) 73
PagI TCATGA 1 cut(s) 733
PfeI GAWTC 4 cut(s) 398, 656, 717, 868
PkrI GCNGC 2 cut(s) 197, 509
PleI GAGTC 1 cut(s) 65
PmaCI CACGTG 1 cut(s) 104
PmlI CACGTG 1 cut(s) 104
PpsI GAGTC 1 cut(s) 65
Ppu21I YACGTR 2 cut(s) 104, 903
Psp124BI GAGCTC 1 cut(s) 23
Psp1406I AACGTT 2 cut(s) 301, 372
Psp6I CCWGG 1 cut(s) 436
PspCI CACGTG 1 cut(s) 104
PspGI CCWGG 1 cut(s) 436
PspPI GGNCC 2 cut(s) 51, 705
PsuI RGATCY 1 cut(s) 126
RsaI GTAC 5 cut(s) 312, 339, 487, 622, 944
RsaNI GTAC 5 cut(s) 311, 338, 486, 621, 943
RseI CAYNNNNRTG 1 cut(s) 901
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 3 cut(s) 281, 369, 687
SatI GCNGC 2 cut(s) 196, 508
Sau3AI GATC 3 cut(s) 126, 287, 847
Sau96I GGNCC 2 cut(s) 51, 705
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 1 cut(s) 438
SduI GDGCHC 1 cut(s) 23
SfaNI GCATC 4 cut(s) 166, 254, 546, 849
Sfr274I CTCGAG 1 cut(s) 73
SinI GGWCC 1 cut(s) 705
SlaI CTCGAG 1 cut(s) 73
SmiMI CAYNNNNRTG 1 cut(s) 901
SmlI CTYRAG 1 cut(s) 73
SmoI CTYRAG 1 cut(s) 73
SphI GCATGC 3 cut(s) 188, 546, 635
Sse9I AATT 6 cut(s) 44, 199, 250, 611, 843, 907
SsiI CCGC 2 cut(s) 546, 618
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 436
TaaI ACNGT 7 cut(s) 232, 342, 578, 593, 665, 783, 914
TaiI ACGT 4 cut(s) 106, 304, 375, 905
TaqI TCGA 4 cut(s) 74, 328, 745, 850
TasI AATT 6 cut(s) 44, 199, 250, 611, 843, 907
TatI WGTACW 3 cut(s) 310, 337, 942
TfiI GAWTC 4 cut(s) 398, 656, 717, 868
Tru1I TTAA 3 cut(s) 281, 369, 687
Tru9I TTAA 3 cut(s) 281, 369, 687
TscAI CASTG 3 cut(s) 297, 596, 769
TseI GCWGC 2 cut(s) 195, 507
TspDTI ATGAA 5 cut(s) 396, 438, 459, 750, 765
TspRI CASTG 3 cut(s) 297, 596, 769
VpaK11BI GGWCC 1 cut(s) 705
XapI RAATTY 2 cut(s) 44, 611
XceI RCATGY 3 cut(s) 188, 546, 635
XcmI CCANNNNNNNNNTGG 1 cut(s) 699
XhoI CTCGAG 1 cut(s) 73
XmiI GTMKAC 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.