Prupe.6G059200_v2.0.a1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0003425
Physical Location & Seq
Reverse (-)
4108449 .. 4109856
1408 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G059200.1

Sequence Viewer

Length: 1389 bp
ATGGCGGAAGTTGGAGAAAGCTCAGGTGCAAAACCTTCTTGCGAAATCTGGGCCAAGCTTGTTCCATCAGACTCGAGATATCCAGATGTTGAGATTAGCTCAGACGAGATTGTAATTTGCTCAGAGATCTTGTTCTCTTCTACTGACAAATGGAAGTGGTGCAAAATAACAAGGAGCTCAGACCATTCTTCTGCCACCATACAAAATAAAAGTTCAAATGCAATATTCGTTGATGGGACTGTAATCCAAGCTGAAGACACTGTTGTTATCAGATGTGGAAGTGAAATTACTTCAGGTCCTGATAAGGAAGGGTACTTAAGCTACAGGTTCAATGTATTGCCTGGTCCTGAAACTTGTCAGAAGCAGTTAAAGATTTCTATGGATGTTGAGCATGCAAAATGCTGCATTTGTCTGAATATATGGCATGAAGTTGTTACTGTTTCTCCTTGCTTCCATAACTTCTGCAATGGATGCTTTTCAGAGTGGTTAAGGAGATCACAGGAGAAACGCTCAAGTGTACTCTGTCCCCAGTGTCGAGCCATTGTCCAATTTGTTGGAAGAAATCATTTCCTGCTTAACATTGCGGAGGAAATACTAAAAGCAGATTCTTCACTAAAACGCTCCGATGAAGAAGTGGCAATTTTAAATTCCTATGCAACAATACGATCAAATCTTGTCGTAAGCTCTGGAAAAAGCCTTCGTGGGAAAAGGGCTCGCTCAGATGTGAACGAGGCAGTTGATGATTCACCACTTCCATGCCCTCAATGTGGTACTGAATTTGGTGGGTTTCGCTGCAATCAAAACACTATTCATCTGCAATGTCAAGCATGCGGAGGAATGATGCCTTCGCGAACCAATATCAGTGTACCTCAACACTGTTCAGGATGTGATAAGGCATTTTGTGGTGCATATTGGAATGCACAAAGGGTTGGACATAGTGATTCTCATCGCATGTGCAGTCGGGAGACTTTTAAACCAATCTCAGAACACACCATCTCTAGAATTCCTACTTCGGCACACGAAAACAATCGACACGAACAAGTTATCACTGAGAAGTGTATTGCACAGTTGGGGAGAACATTGCAGGATGTTATAGCAGAATGGATTGCAAAGTTGAACAACAGAGAAATTGACCGGACAAGGATGCCGCTGAATCATGCTGAGATGATAACTTCTGGGACACCTACGTGCAACAATTGTTATGAGAAATTGATCTCATTCCTCTTGTACTGGTTTCGGATTTCGATTCCCAAAAATCATCTACCTCCGGAGGCATCAAACAGGGAAAATTGCTGGTACGGATATGCATGTCGAACGCAGCACCATAATGAAGAACATGCTCGCAAAAGAAATCATGTTTGCCGGCCAACTAAGGGTTCTAATATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000209 GO:0000278 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016604 GO:0016605 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031647 GO:0031648 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0036094 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045732 GO:0045786 GO:0045862 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051726 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0097159 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1903047 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_2

Protein Analysis

463

Amino Acids

52.06

Weight (kDa)

7.9

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 850
AccIII TCCGGA 1 cut(s) 1267
AciI CCGC 4 cut(s) 5, 584, 831, 1148
AcoI YGGCCR 1 cut(s) 1364
AcsI RAATTY 3 cut(s) 646, 776, 1002
AcuI CTGAAG 2 cut(s) 273, 276
AfaI GTAC 6 cut(s) 314, 519, 772, 867, 1229, 1298
AfiI CCNNNNNNNGG 2 cut(s) 767, 1373
AflII CTTAAG 1 cut(s) 316
AgsI TTSAA 3 cut(s) 216, 331, 1117
AjnI CCWGG 1 cut(s) 340
AjuI GAANNNNNNNTTGG 1 cut(s) 1360
AleI CACNNNNGTG 1 cut(s) 1186
AluBI AGCT 7 cut(s) 21, 58, 99, 177, 251, 321, 684
AluI AGCT 7 cut(s) 21, 58, 99, 177, 251, 321, 684
Alw21I GWGCWC 1 cut(s) 179
Alw26I GTCTC 1 cut(s) 959
AlwNI CAGNNNCTG 1 cut(s) 299
Ama87I CYCGRG 1 cut(s) 73
Aor13HI TCCGGA 1 cut(s) 1267
AoxI GGCC 2 cut(s) 51, 1364
ApeKI GCWGC 3 cut(s) 402, 792, 1318
ApoI RAATTY 3 cut(s) 646, 776, 1002
AspS9I GGNCC 3 cut(s) 51, 296, 344
AsuHPI GGTGA 1 cut(s) 738
AvaI CYCGRG 1 cut(s) 73
AvaII GGWCC 2 cut(s) 296, 344
BanII GRGCYC 2 cut(s) 179, 715
BarI GAAGNNNNNNTAC 2 cut(s) 271, 303
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 179
BbvI GCAGC 3 cut(s) 389, 779, 1330
BccI CCATC 3 cut(s) 73, 227, 1001
BciT130I CCWGG 1 cut(s) 342
BcoDI GTCTC 1 cut(s) 959
BfaI CTAG 1 cut(s) 999
BfmI CTRYAG 1 cut(s) 322
BfrI CTTAAG 1 cut(s) 316
BglII AGATCT 1 cut(s) 126
BisI GCNGC 4 cut(s) 403, 793, 1148, 1319
BlsI GCNGC 4 cut(s) 404, 794, 1149, 1320
Bme1390I CCNGG 1 cut(s) 342
Bme18I GGWCC 2 cut(s) 296, 344
BmeT110I CYCGRG 1 cut(s) 73
BmgT120I GGNCC 3 cut(s) 51, 296, 344
BmrFI CCNGG 1 cut(s) 342
BmrI ACTGGG 1 cut(s) 523
BmsI GCATC 4 cut(s) 461, 831, 1134, 1283
BmuI ACTGGG 1 cut(s) 523
BpiI GAAGAC 1 cut(s) 261
BplI GAGNNNNNCTC 4 cut(s) 83, 115, 494, 526
Bpu10I CCTNAGC 1 cut(s) 22
BpuEI CTTGAG 1 cut(s) 496
BsaAI YACGTR 1 cut(s) 1188
BsaBI GATNNNNATC 1 cut(s) 945
BsaWI WCCGGW 2 cut(s) 1134, 1267
BsaXI ACNNNNNCTCC 2 cut(s) 427, 457
Bsc4I CCNNNNNNNGG 2 cut(s) 767, 1373
Bse118I RCCGGY 1 cut(s) 1362
Bse1I ACTGG 2 cut(s) 529, 1235
Bse3DI GCAATG 4 cut(s) 472, 579, 824, 1079
Bse8I GATNNNNATC 1 cut(s) 945
BseAI TCCGGA 1 cut(s) 1267
BseBI CCWGG 1 cut(s) 342
BseGI GGATG 5 cut(s) 388, 476, 890, 1093, 1149
BseJI GATNNNNATC 1 cut(s) 945
BseLI CCNNNNNNNGG 2 cut(s) 767, 1373
BseMI GCAATG 4 cut(s) 472, 579, 824, 1079
BseMII CTCAG 8 cut(s) 36, 114, 135, 192, 732, 996, 1041, 1152
BseNI ACTGG 2 cut(s) 529, 1235
BseXI GCAGC 3 cut(s) 389, 779, 1330
BsgI GTGCAG 1 cut(s) 976
Bsh1236I CGCG 1 cut(s) 850
BshFI GGCC 2 cut(s) 53, 1366
BsiHKAI GWGCWC 1 cut(s) 179
BsiHKCI CYCGRG 1 cut(s) 73
BsiSI CCGG 3 cut(s) 1135, 1268, 1363
BslFI GGGAC 3 cut(s) 250, 510, 1192
BslI CCNNNNNNNGG 2 cut(s) 767, 1373
BsmAI GTCTC 1 cut(s) 959
BsmFI GGGAC 3 cut(s) 250, 510, 1192
BsmI GAATGC 1 cut(s) 922
BsnI GGCC 2 cut(s) 53, 1366
BsoBI CYCGRG 1 cut(s) 73
Bsp1286I GDGCHC 2 cut(s) 179, 715
Bsp13I TCCGGA 1 cut(s) 1267
Bsp143I GATC 4 cut(s) 126, 494, 665, 1212
Bsp68I TCGCGA 1 cut(s) 850
BspACI CCGC 4 cut(s) 5, 584, 831, 1148
BspANI GGCC 2 cut(s) 53, 1366
BspCNI CTCAG 8 cut(s) 35, 113, 134, 191, 731, 995, 1042, 1153
BspEI TCCGGA 1 cut(s) 1267
BspFNI CGCG 1 cut(s) 850
BspTI CTTAAG 1 cut(s) 316
BsrDI GCAATG 4 cut(s) 472, 579, 824, 1079
BsrFI RCCGGY 1 cut(s) 1362
BsrI ACTGG 2 cut(s) 529, 1235
BssAI RCCGGY 1 cut(s) 1362
BssMI GATC 4 cut(s) 126, 494, 665, 1212
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 5 cut(s) 241, 262, 439, 878, 1068
Bst6I CTCTTC 1 cut(s) 142
BstAFI CTTAAG 1 cut(s) 316
BstAPI GCANNNNNTGC 1 cut(s) 471
BstBAI YACGTR 1 cut(s) 1188
BstC8I GCNNGC 5 cut(s) 393, 715, 829, 1342, 1364
BstDEI CTNAG 9 cut(s) 22, 100, 121, 178, 718, 982, 1050, 1161, 1371
BstF5I GGATG 5 cut(s) 388, 476, 890, 1093, 1149
BstFNI CGCG 1 cut(s) 850
BstKTI GATC 4 cut(s) 129, 497, 668, 1215
BstMAI GTCTC 1 cut(s) 959
BstMBI GATC 4 cut(s) 126, 494, 665, 1212
BstMWI GCNNNNNNNGC 1 cut(s) 471
BstNI CCWGG 1 cut(s) 342
BstNSI RCATGY 5 cut(s) 395, 831, 955, 1311, 1340
BstSCI CCNGG 1 cut(s) 340
BstSFI CTRYAG 1 cut(s) 322
BstUI CGCG 1 cut(s) 850
BstV1I GCAGC 3 cut(s) 389, 779, 1330
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 126
BstXI CCANNNNNNTGG 1 cut(s) 554
BstYI RGATCY 1 cut(s) 126
BsuRI GGCC 2 cut(s) 53, 1366
BtgZI GCGATG 1 cut(s) 932
BtsCI GGATG 5 cut(s) 388, 476, 890, 1093, 1149
BtsIMutI CAGTG 5 cut(s) 258, 536, 868, 874, 1047
BtuMI TCGCGA 1 cut(s) 850
Cac8I GCNNGC 5 cut(s) 393, 715, 829, 1342, 1364
CaiI CAGNNNCTG 1 cut(s) 299
Cfr10I RCCGGY 1 cut(s) 1362
Cfr13I GGNCC 3 cut(s) 51, 296, 344
Csp6I GTAC 6 cut(s) 313, 518, 771, 866, 1228, 1297
CviAII CATG 9 cut(s) 392, 425, 756, 828, 952, 1157, 1308, 1337, 1355
CviQI GTAC 6 cut(s) 313, 518, 771, 866, 1228, 1297
DdeI CTNAG 9 cut(s) 22, 100, 121, 178, 718, 982, 1050, 1161, 1371
DpnI GATC 4 cut(s) 128, 496, 667, 1214
DpnII GATC 4 cut(s) 126, 494, 665, 1212
DraI TTTAAA 2 cut(s) 645, 973
EaeI YGGCCR 1 cut(s) 1364
Eam1104I CTCTTC 1 cut(s) 142
EarI CTCTTC 1 cut(s) 142
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 177
Eco24I GRGCYC 2 cut(s) 179, 715
Eco32I GATATC 1 cut(s) 80
Eco47I GGWCC 2 cut(s) 296, 344
Eco53kI GAGCTC 1 cut(s) 177
Eco57I CTGAAG 2 cut(s) 273, 276
Eco88I CYCGRG 1 cut(s) 73
EcoICRI GAGCTC 1 cut(s) 177
EcoO109I RGGNCCY 1 cut(s) 296
EcoRI GAATTC 1 cut(s) 1002
EcoRII CCWGG 1 cut(s) 340
EcoRV GATATC 1 cut(s) 80
EcoT22I ATGCAT 1 cut(s) 1309
EcoT38I GRGCYC 2 cut(s) 179, 715
FaeI CATG 9 cut(s) 395, 428, 759, 831, 955, 1160, 1311, 1340, 1358
FaqI GGGAC 3 cut(s) 250, 510, 1192
FatI CATG 9 cut(s) 391, 424, 755, 827, 951, 1156, 1307, 1336, 1354
Fnu4HI GCNGC 4 cut(s) 403, 793, 1148, 1319
FokI GGATG 5 cut(s) 395, 483, 897, 1100, 1156
FriOI GRGCYC 2 cut(s) 179, 715
Fsp4HI GCNGC 4 cut(s) 403, 793, 1148, 1319
FspBI CTAG 1 cut(s) 999
GluI GCNGC 4 cut(s) 403, 793, 1148, 1319
HaeIII GGCC 2 cut(s) 53, 1366
HapII CCGG 3 cut(s) 1135, 1268, 1363
Hin1II CATG 9 cut(s) 395, 428, 759, 831, 955, 1160, 1311, 1340, 1358
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 6 cut(s) 71, 605, 743, 941, 1153, 1246
HpaII CCGG 3 cut(s) 1135, 1268, 1363
HphI GGTGA 1 cut(s) 738
Hpy166II GTNNAC 3 cut(s) 518, 727, 866
Hpy8I GTNNAC 3 cut(s) 518, 727, 866
HpyAV CCTTC 4 cut(s) 45, 302, 707, 855
HpyCH4III ACNGT 5 cut(s) 241, 262, 439, 878, 1068
HpyCH4IV ACGT 1 cut(s) 1187
HpyF10VI GCNNNNNNNGC 1 cut(s) 471
HpyF3I CTNAG 9 cut(s) 22, 100, 121, 178, 718, 982, 1050, 1161, 1371
HpySE526I ACGT 1 cut(s) 1187
Hsp92II CATG 9 cut(s) 395, 428, 759, 831, 955, 1160, 1311, 1340, 1358
Kpn2I TCCGGA 1 cut(s) 1267
KroI GCCGGC 1 cut(s) 1362
KroNI GCCGGC 1 cut(s) 1364
Kzo9I GATC 4 cut(s) 126, 494, 665, 1212
LmnI GCTCC 2 cut(s) 174, 626
Lsp1109I GCAGC 3 cut(s) 389, 779, 1330
LweI GCATC 4 cut(s) 461, 831, 1134, 1283
MaeI CTAG 1 cut(s) 999
MaeII ACGT 1 cut(s) 1187
MaeIII GTNAC 1 cut(s) 433
MalI GATC 4 cut(s) 128, 496, 667, 1214
MboI GATC 4 cut(s) 126, 494, 665, 1212
MboII GAAGA 7 cut(s) 129, 180, 266, 570, 600, 641, 1343
MfeI CAATTG 1 cut(s) 1195
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 2 cut(s) 179, 715
MlyI GAGTC 1 cut(s) 65
MmeI TCCRAC 2 cut(s) 535, 910
MnlI CCTC 8 cut(s) 580, 724, 771, 827, 879, 1232, 1264, 1275
Mph1103I ATGCAT 1 cut(s) 1309
MroI TCCGGA 1 cut(s) 1267
MroNI GCCGGC 1 cut(s) 1362
MseI TTAA 6 cut(s) 317, 368, 488, 576, 644, 972
MslI CAYNNNNRTG 3 cut(s) 754, 1186, 1326
MspA1I CMGCKG 1 cut(s) 1150
MspCI CTTAAG 1 cut(s) 316
MspI CCGG 3 cut(s) 1135, 1268, 1363
MspR9I CCNGG 1 cut(s) 342
MunI CAATTG 1 cut(s) 1195
Mva1269I GAATGC 1 cut(s) 922
MvaI CCWGG 1 cut(s) 342
MvnI CGCG 1 cut(s) 850
MwoI GCNNNNNNNGC 1 cut(s) 471
NaeI GCCGGC 1 cut(s) 1364
NdeII GATC 4 cut(s) 126, 494, 665, 1212
NgoMIV GCCGGC 1 cut(s) 1362
NlaIII CATG 9 cut(s) 395, 428, 759, 831, 955, 1160, 1311, 1340, 1358
NruI TCGCGA 1 cut(s) 850
NsiI ATGCAT 1 cut(s) 1309
NspI RCATGY 5 cut(s) 395, 831, 955, 1311, 1340
OliI CACNNNNGTG 1 cut(s) 1186
PaeI GCATGC 2 cut(s) 395, 831
PaeR7I CTCGAG 1 cut(s) 73
PctI GAATGC 1 cut(s) 922
PdiI GCCGGC 1 cut(s) 1364
PfeI GAWTC 5 cut(s) 605, 743, 941, 1153, 1246
PkrI GCNGC 4 cut(s) 404, 794, 1149, 1320
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
Ppu21I YACGTR 1 cut(s) 1188
PpuMI RGGWCCY 1 cut(s) 296
Psp124BI GAGCTC 1 cut(s) 179
Psp5II RGGWCCY 1 cut(s) 296
Psp6I CCWGG 1 cut(s) 340
PspGI CCWGG 1 cut(s) 340
PspPI GGNCC 3 cut(s) 51, 296, 344
PspPPI RGGWCCY 1 cut(s) 296
PstNI CAGNNNCTG 1 cut(s) 299
PsuI RGATCY 1 cut(s) 126
RruI TCGCGA 1 cut(s) 850
RsaI GTAC 6 cut(s) 314, 519, 772, 867, 1229, 1298
RsaNI GTAC 6 cut(s) 313, 518, 771, 866, 1228, 1297
RseI CAYNNNNRTG 3 cut(s) 754, 1186, 1326
SacI GAGCTC 1 cut(s) 179
SaqAI TTAA 6 cut(s) 317, 368, 488, 576, 644, 972
SatI GCNGC 4 cut(s) 403, 793, 1148, 1319
Sau3AI GATC 4 cut(s) 126, 494, 665, 1212
Sau96I GGNCC 3 cut(s) 51, 296, 344
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 1 cut(s) 342
SduI GDGCHC 2 cut(s) 179, 715
SfaNI GCATC 4 cut(s) 461, 831, 1134, 1283
SfcI CTRYAG 1 cut(s) 322
Sfr274I CTCGAG 1 cut(s) 73
SinI GGWCC 2 cut(s) 296, 344
SlaI CTCGAG 1 cut(s) 73
SmiMI CAYNNNNRTG 3 cut(s) 754, 1186, 1326
SmlI CTYRAG 3 cut(s) 73, 316, 511
SmoI CTYRAG 3 cut(s) 73, 316, 511
SphI GCATGC 2 cut(s) 395, 831
SsiI CCGC 4 cut(s) 5, 584, 831, 1148
SspI AATATT 1 cut(s) 225
SspMI CTAG 1 cut(s) 999
SstI GAGCTC 1 cut(s) 179
StyD4I CCNGG 1 cut(s) 340
TaaI ACNGT 5 cut(s) 241, 262, 439, 878, 1068
TaiI ACGT 1 cut(s) 1190
TaqI TCGA 5 cut(s) 74, 535, 1030, 1244, 1312
TatI WGTACW 2 cut(s) 517, 1227
TauI GCSGC 1 cut(s) 1150
TfiI GAWTC 5 cut(s) 605, 743, 941, 1153, 1246
Tru1I TTAA 6 cut(s) 317, 368, 488, 576, 644, 972
Tru9I TTAA 6 cut(s) 317, 368, 488, 576, 644, 972
TscAI CASTG 5 cut(s) 265, 536, 868, 881, 1054
TseI GCWGC 3 cut(s) 402, 792, 1318
TspDTI ATGAA 4 cut(s) 441, 642, 800, 1344
TspGWI ACGGA 1 cut(s) 1314
TspRI CASTG 5 cut(s) 265, 536, 868, 881, 1054
Vha464I CTTAAG 1 cut(s) 316
VpaK11BI GGWCC 2 cut(s) 296, 344
XapI RAATTY 3 cut(s) 646, 776, 1002
XbaI TCTAGA 1 cut(s) 998
XceI RCATGY 5 cut(s) 395, 831, 955, 1311, 1340
XhoI CTCGAG 1 cut(s) 73
XspI CTAG 1 cut(s) 999
Zsp2I ATGCAT 1 cut(s) 1309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.