Rroxscaffold_1G00011750

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003425
Physical Location & Seq
Reverse (-)
14721881 .. 14724196
2316 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00011750.1

Sequence Viewer

Length: 1422 bp
ATGGCAGAAGTTGGAGAAAGCTCTGGTGCAAAGCCCTCTGGTGAAATCTGGGCCAAGCTTGTTCCTTCTGACTCGAGATTTCCAGATGTTGAGATCAGAGATCTTGTTCTGACTCAAGATACCCCTCTGAGTCAAGATATTGGTTCAGATGAGAGTTTAGTTTGCTCTCCTATTGACAAATGCGAGTGGTGCAGAATTACGAGGAACCCTGATCAGTCTTCTGCTACTATACAAAATAAGAGTTCAAATGCAATACTTGTTGATGGAGCTGTGATCCAATCTGAAGAGAGTACTGTTATCAGATCTGGAAGTGAAATTATTTCAGGTCCTGAGAGAGAAGGGTGTTTAAGCTACAGATTCAATGTGTTGCCTGGTCCTGAAACTTGTCAGAAGATGCTGAAGATTAGTGTGGATGCTGAGCATGCAAAGTGCTGCATTTGTTTAAATATATGGCATGATGCTGTTACCATTGCTCCTTGCTTCCATAACTTCTGCAATGGGTGCTTCTCAGAGTGGTTAAGGAGATCACAGGAGAAACGTTCAAGTGTACTCTGTCCCCAATGTCGAGCAGTTGTGCAATTTGTTGGAAGAAACCACTTCCTGCATAACATTGCCGATGAAATACTGAATGCGGATTCTTCACTGAGACGCTCCAACGAAGACACTGCAGTTTTAGATTCCTATGCAACAGTACGATCAAATCTGGTAATAAAGTCGGGAAAAGGGCTGCTTGGGAAAAGGGTTCGTTCAGCAGTGCAAGATGAAGCTGATAGTGTAGAGCTTCCATGCCCTCAATGCAGTAATCAATTTGGTGGGTTTCTCTGCAACCGGAGCACTACCCATCTGCAATGTCAAGCATGTGGAGGACTGATGCCTTCACGAACCAATACAAGTGTACCTCAACACTGTATAGGGTGTGATAGGTCATTTTGTGGTGCATATTGGCATGCTCAAGGTGTTGCACAAAGTGCTTCTCACCGCATATGCAGTCGTGATACTTTCAAACCAATCTCAGAACGCACCATCTCTAGAATTCCTACTTCAGCACATGAAAACAATCGACATGAACAAGAAATCACTATGAGGTGTATTGCACAAATGGGAAGAACATTGCAGGATGTTATTGCAGAATGGATGGCAATGTTCAATAACAGAACACTTGATCGAACTAACAGGATGCCACTGAAGCATGCCGAGCTGATAACTTCTGGGACATATACATGCAGTGATTGTTATGAGAAACTGATCTCATTCCTCTTGTACTGGTTTCGGGTTTCAGTTTCTGCATCTCATCTTCCCCAAGATGCATCAAAAAGGGAAAATTGCTGGTATGGATATGCGTGTCGAACACAGCACCATAATGAAGACCATGCTCGTAAAAGGAATCATGTATGCCGGCCGACTAGGGGTGCTAATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000209 GO:0000278 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016604 GO:0016605 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031647 GO:0031648 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0036094 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045732 GO:0045786 GO:0045862 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051726 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0097159 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1903047 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_2

Protein Analysis

473

Amino Acids

52.88

Weight (kDa)

7.46

Isoelectric Point (pI)

53.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 143 - 189 3.5e-07 Ring finger domain
zf-C3HC4_2 PF13923 143 - 188 9.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 144 - 188 2.8e-06 Zinc finger, C3HC4 type (RING finger)
zf-CRD PF17979 263 - 426 8.1e-48 Cysteine rich domain with multizinc binding regions
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 632, 979
AclI AACGTT 1 cut(s) 538
AclWI GGATC 1 cut(s) 268
AcoI YGGCCR 1 cut(s) 1397
AcsI RAATTY 1 cut(s) 1032
AcuI CTGAAG 4 cut(s) 303, 419, 1026, 1205
AdeI CACNNNGTG 1 cut(s) 968
AfaI GTAC 5 cut(s) 292, 549, 693, 897, 1262
AfiI CCNNNNNNNGG 1 cut(s) 1406
AgsI TTSAA 5 cut(s) 246, 361, 543, 1003, 1147
AjnI CCWGG 1 cut(s) 370
AluBI AGCT 7 cut(s) 21, 58, 269, 351, 767, 781, 1198
AluI AGCT 7 cut(s) 21, 58, 269, 351, 767, 781, 1198
Alw21I GWGCWC 1 cut(s) 836
Alw26I GTCTC 1 cut(s) 640
AlwI GGATC 1 cut(s) 268
AlwNI CAGNNNCTG 2 cut(s) 329, 1283
Ama87I CYCGRG 1 cut(s) 73
AoxI GGCC 2 cut(s) 51, 1397
ApeKI GCWGC 2 cut(s) 432, 727
ApoI RAATTY 1 cut(s) 1032
AspS9I GGNCC 3 cut(s) 51, 326, 374
AsuHPI GGTGA 2 cut(s) 53, 968
AvaI CYCGRG 1 cut(s) 73
AvaII GGWCC 2 cut(s) 326, 374
BaeI ACNNNNGTAYC 2 cut(s) 879, 912
BbsI GAAGAC 3 cut(s) 210, 666, 1371
Bbv12I GWGCWC 1 cut(s) 836
BbvI GCAGC 2 cut(s) 419, 714
BccI CCATC 4 cut(s) 257, 849, 1031, 1129
BcgI CGANNNNNNTGC 2 cut(s) 647, 681
BciT130I CCWGG 1 cut(s) 372
BclI TGATCA 1 cut(s) 211
BcoDI GTCTC 1 cut(s) 640
BfaI CTAG 2 cut(s) 1029, 1404
BfmI CTRYAG 2 cut(s) 352, 666
BglII AGATCT 2 cut(s) 100, 302
BisI GCNGC 2 cut(s) 433, 728
BlpI GCTNAGC 1 cut(s) 417
BlsI GCNGC 2 cut(s) 434, 729
BmcAI AGTACT 1 cut(s) 292
Bme1390I CCNGG 1 cut(s) 372
Bme18I GGWCC 2 cut(s) 326, 374
BmeT110I CYCGRG 1 cut(s) 73
BmgT120I GGNCC 3 cut(s) 51, 326, 374
BmiI GGNNCC 1 cut(s) 206
BmrFI CCNGG 1 cut(s) 372
BmsI GCATC 8 cut(s) 384, 403, 448, 861, 1167, 1294, 1295, 1316
BpiI GAAGAC 3 cut(s) 210, 666, 1371
Bpu1102I GCTNAGC 1 cut(s) 417
BpuEI CTTGAG 2 cut(s) 99, 936
BsaWI WCCGGW 1 cut(s) 828
BsaXI ACNNNNNCTCC 2 cut(s) 457, 487
Bsc4I CCNNNNNNNGG 1 cut(s) 1406
Bse118I RCCGGY 1 cut(s) 1395
Bse1I ACTGG 1 cut(s) 1268
Bse3DI GCAATG 6 cut(s) 468, 502, 609, 854, 1109, 1146
BseBI CCWGG 1 cut(s) 372
BseGI GGATG 4 cut(s) 418, 1123, 1140, 1182
BseLI CCNNNNNNNGG 1 cut(s) 1406
BseMI GCAATG 6 cut(s) 468, 502, 609, 854, 1109, 1146
BseMII CTCAG 6 cut(s) 119, 321, 408, 522, 635, 1026
BseNI ACTGG 1 cut(s) 1268
BseX3I CGGCCG 1 cut(s) 1397
BseXI GCAGC 2 cut(s) 419, 714
BsgI GTGCAG 1 cut(s) 211
Bsh1285I CGRYCG 1 cut(s) 1400
BshFI GGCC 2 cut(s) 53, 1399
BsiEI CGRYCG 1 cut(s) 1400
BsiHKAI GWGCWC 1 cut(s) 836
BsiHKCI CYCGRG 1 cut(s) 73
BsiSI CCGG 2 cut(s) 829, 1396
BslFI GGGAC 2 cut(s) 540, 1225
BslI CCNNNNNNNGG 1 cut(s) 1406
BsmAI GTCTC 1 cut(s) 640
BsmBI CGTCTC 1 cut(s) 640
BsmFI GGGAC 2 cut(s) 540, 1225
BsmI GAATGC 1 cut(s) 634
BsnI GGCC 2 cut(s) 53, 1399
BsoBI CYCGRG 1 cut(s) 73
Bsp1286I GDGCHC 1 cut(s) 836
Bsp143I GATC 9 cut(s) 93, 100, 211, 273, 302, 524, 695, 1162, 1245
Bsp1720I GCTNAGC 1 cut(s) 417
BspACI CCGC 2 cut(s) 632, 979
BspANI GGCC 2 cut(s) 53, 1399
BspCNI CTCAG 6 cut(s) 120, 322, 409, 521, 636, 1025
BspLI GGNNCC 1 cut(s) 206
BspMAI CTGCAG 1 cut(s) 670
BspPI GGATC 1 cut(s) 268
BsrDI GCAATG 6 cut(s) 468, 502, 609, 854, 1109, 1146
BsrFI RCCGGY 1 cut(s) 1395
BsrI ACTGG 1 cut(s) 1268
BssAI RCCGGY 1 cut(s) 1395
BssMI GATC 9 cut(s) 93, 100, 211, 273, 302, 524, 695, 1162, 1245
Bst2UI CCWGG 1 cut(s) 372
Bst4CI ACNGT 3 cut(s) 295, 691, 908
Bst6I CTCTTC 1 cut(s) 279
BstAPI GCANNNNNTGC 2 cut(s) 501, 968
BstC8I GCNNGC 4 cut(s) 423, 948, 1191, 1397
BstDEI CTNAG 6 cut(s) 128, 330, 417, 508, 644, 1012
BstF5I GGATG 4 cut(s) 418, 1123, 1140, 1182
BstKTI GATC 9 cut(s) 96, 103, 214, 276, 305, 527, 698, 1165, 1248
BstMAI GTCTC 1 cut(s) 640
BstMBI GATC 9 cut(s) 93, 100, 211, 273, 302, 524, 695, 1162, 1245
BstMCI CGRYCG 1 cut(s) 1400
BstMWI GCNNNNNNNGC 8 cut(s) 189, 422, 501, 795, 831, 968, 1186, 1195
BstNI CCWGG 1 cut(s) 372
BstNSI RCATGY 5 cut(s) 425, 861, 950, 1193, 1224
BstSCI CCNGG 1 cut(s) 370
BstSFI CTRYAG 2 cut(s) 352, 666
BstV1I GCAGC 2 cut(s) 419, 714
BstV2I GAAGAC 3 cut(s) 210, 666, 1371
BstX2I RGATCY 2 cut(s) 100, 302
BstYI RGATCY 2 cut(s) 100, 302
BstZI CGGCCG 1 cut(s) 1397
BsuRI GGCC 2 cut(s) 53, 1399
BtsCI GGATG 4 cut(s) 418, 1123, 1140, 1182
BtsI GCAGTG 3 cut(s) 663, 759, 1231
BtsIMutI CAGTG 6 cut(s) 641, 663, 759, 904, 1181, 1231
Cac8I GCNNGC 4 cut(s) 423, 948, 1191, 1397
CaiI CAGNNNCTG 2 cut(s) 329, 1283
Cfr10I RCCGGY 1 cut(s) 1395
Cfr13I GGNCC 3 cut(s) 51, 326, 374
CseI GACGC 1 cut(s) 657
Csp6I GTAC 5 cut(s) 291, 548, 692, 896, 1261
CviQI GTAC 5 cut(s) 291, 548, 692, 896, 1261
DdeI CTNAG 6 cut(s) 128, 330, 417, 508, 644, 1012
DpnI GATC 9 cut(s) 95, 102, 213, 275, 304, 526, 697, 1164, 1247
DpnII GATC 9 cut(s) 93, 100, 211, 273, 302, 524, 695, 1162, 1245
DraI TTTAAA 1 cut(s) 444
DraIII CACNNNGTG 1 cut(s) 968
EaeI YGGCCR 1 cut(s) 1397
EagI CGGCCG 1 cut(s) 1397
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
EclXI CGGCCG 1 cut(s) 1397
Eco47I GGWCC 2 cut(s) 326, 374
Eco52I CGGCCG 1 cut(s) 1397
Eco57I CTGAAG 4 cut(s) 303, 419, 1026, 1205
Eco88I CYCGRG 1 cut(s) 73
EcoO109I RGGNCCY 1 cut(s) 326
EcoRI GAATTC 1 cut(s) 1032
EcoRII CCWGG 1 cut(s) 370
EcoT22I ATGCAT 1 cut(s) 1309
Esp3I CGTCTC 1 cut(s) 640
FalI AAGNNNNNCTT 2 cut(s) 714, 746
FaqI GGGAC 2 cut(s) 540, 1225
FauNDI CATATG 1 cut(s) 983
FbaI TGATCA 1 cut(s) 211
Fnu4HI GCNGC 2 cut(s) 433, 728
FokI GGATG 4 cut(s) 425, 1130, 1147, 1189
Fsp4HI GCNGC 2 cut(s) 433, 728
FspBI CTAG 2 cut(s) 1029, 1404
GluI GCNGC 2 cut(s) 433, 728
HaeIII GGCC 2 cut(s) 53, 1399
HapII CCGG 2 cut(s) 829, 1396
HgaI GACGC 1 cut(s) 657
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 7 cut(s) 71, 112, 130, 357, 635, 677, 1384
HpaII CCGG 2 cut(s) 829, 1396
HphI GGTGA 2 cut(s) 53, 968
Hpy166II GTNNAC 2 cut(s) 548, 896
Hpy8I GTNNAC 2 cut(s) 548, 896
HpyAV CCTTC 3 cut(s) 75, 332, 885
HpyCH4III ACNGT 3 cut(s) 295, 691, 908
HpyCH4IV ACGT 1 cut(s) 538
HpyF10VI GCNNNNNNNGC 8 cut(s) 189, 422, 501, 795, 831, 968, 1186, 1195
HpyF3I CTNAG 6 cut(s) 128, 330, 417, 508, 644, 1012
HpySE526I ACGT 1 cut(s) 538
KroI GCCGGC 1 cut(s) 1395
KroNI GCCGGC 1 cut(s) 1397
Ksp22I TGATCA 1 cut(s) 211
Kzo9I GATC 9 cut(s) 93, 100, 211, 273, 302, 524, 695, 1162, 1245
LmnI GCTCC 4 cut(s) 266, 478, 656, 831
Lsp1109I GCAGC 2 cut(s) 419, 714
LweI GCATC 8 cut(s) 384, 403, 448, 861, 1167, 1294, 1295, 1316
MaeI CTAG 2 cut(s) 1029, 1404
MaeII ACGT 1 cut(s) 538
MaeIII GTNAC 1 cut(s) 463
MalI GATC 9 cut(s) 95, 102, 213, 275, 304, 526, 697, 1164, 1247
MboI GATC 9 cut(s) 93, 100, 211, 273, 302, 524, 695, 1162, 1245
MflI RGATCY 2 cut(s) 100, 302
MhlI GDGCHC 1 cut(s) 836
MluCI AATT 6 cut(s) 195, 315, 578, 806, 1032, 1321
MlyI GAGTC 3 cut(s) 65, 106, 139
MmeI TCCRAC 2 cut(s) 565, 678
MnlI CCTC 8 cut(s) 46, 135, 195, 801, 857, 909, 1077, 1265
Mph1103I ATGCAT 1 cut(s) 1309
MroNI GCCGGC 1 cut(s) 1395
MseI TTAA 3 cut(s) 347, 443, 518
MslI CAYNNNNRTG 2 cut(s) 1219, 1359
MspI CCGG 2 cut(s) 829, 1396
MspR9I CCNGG 1 cut(s) 372
Mva1269I GAATGC 1 cut(s) 634
MvaI CCWGG 1 cut(s) 372
MwoI GCNNNNNNNGC 8 cut(s) 189, 422, 501, 795, 831, 968, 1186, 1195
NaeI GCCGGC 1 cut(s) 1397
NdeI CATATG 1 cut(s) 983
NdeII GATC 9 cut(s) 93, 100, 211, 273, 302, 524, 695, 1162, 1245
NgoMIV GCCGGC 1 cut(s) 1395
NlaIV GGNNCC 1 cut(s) 206
NmeAIII GCCGAG 1 cut(s) 1219
NsiI ATGCAT 1 cut(s) 1309
NspI RCATGY 5 cut(s) 425, 861, 950, 1193, 1224
PaeI GCATGC 3 cut(s) 425, 950, 1193
PaeR7I CTCGAG 1 cut(s) 73
PctI GAATGC 1 cut(s) 634
PdiI GCCGGC 1 cut(s) 1397
PfeI GAWTC 4 cut(s) 357, 635, 677, 1384
PkrI GCNGC 2 cut(s) 434, 729
PleI GAGTC 3 cut(s) 65, 106, 138
PpsI GAGTC 3 cut(s) 65, 106, 138
PpuMI RGGWCCY 1 cut(s) 326
Psp1406I AACGTT 1 cut(s) 538
Psp5II RGGWCCY 1 cut(s) 326
Psp6I CCWGG 1 cut(s) 370
PspGI CCWGG 1 cut(s) 370
PspN4I GGNNCC 1 cut(s) 206
PspPI GGNCC 3 cut(s) 51, 326, 374
PspPPI RGGWCCY 1 cut(s) 326
PstI CTGCAG 1 cut(s) 670
PstNI CAGNNNCTG 2 cut(s) 329, 1283
PsuI RGATCY 2 cut(s) 100, 302
RsaI GTAC 5 cut(s) 292, 549, 693, 897, 1262
RsaNI GTAC 5 cut(s) 291, 548, 692, 896, 1261
RseI CAYNNNNRTG 2 cut(s) 1219, 1359
SaqAI TTAA 3 cut(s) 347, 443, 518
SatI GCNGC 2 cut(s) 433, 728
Sau3AI GATC 9 cut(s) 93, 100, 211, 273, 302, 524, 695, 1162, 1245
Sau96I GGNCC 3 cut(s) 51, 326, 374
ScaI AGTACT 1 cut(s) 292
SchI GAGTC 3 cut(s) 65, 106, 139
ScrFI CCNGG 1 cut(s) 372
SduI GDGCHC 1 cut(s) 836
SfaNI GCATC 8 cut(s) 384, 403, 448, 861, 1167, 1294, 1295, 1316
SfcI CTRYAG 2 cut(s) 352, 666
Sfr274I CTCGAG 1 cut(s) 73
SinI GGWCC 2 cut(s) 326, 374
SlaI CTCGAG 1 cut(s) 73
SmiMI CAYNNNNRTG 2 cut(s) 1219, 1359
SmlI CTYRAG 3 cut(s) 73, 114, 951
SmoI CTYRAG 3 cut(s) 73, 114, 951
SphI GCATGC 3 cut(s) 425, 950, 1193
Sse9I AATT 6 cut(s) 195, 315, 578, 806, 1032, 1321
SsiI CCGC 2 cut(s) 632, 979
SspMI CTAG 2 cut(s) 1029, 1404
StyD4I CCNGG 1 cut(s) 370
TaaI ACNGT 3 cut(s) 295, 691, 908
TaiI ACGT 1 cut(s) 541
TaqI TCGA 5 cut(s) 74, 565, 1060, 1165, 1345
TasI AATT 6 cut(s) 195, 315, 578, 806, 1032, 1321
TatI WGTACW 3 cut(s) 290, 547, 1260
TfiI GAWTC 4 cut(s) 357, 635, 677, 1384
Tru1I TTAA 3 cut(s) 347, 443, 518
Tru9I TTAA 3 cut(s) 347, 443, 518
TscAI CASTG 6 cut(s) 648, 670, 759, 911, 1188, 1231
TseI GCWGC 2 cut(s) 432, 727
TspDTI ATGAA 5 cut(s) 633, 777, 1065, 1080, 1377
TspRI CASTG 6 cut(s) 648, 670, 759, 911, 1188, 1231
VpaK11BI GGWCC 2 cut(s) 326, 374
XapI RAATTY 1 cut(s) 1032
XbaI TCTAGA 1 cut(s) 1028
XceI RCATGY 5 cut(s) 425, 861, 950, 1193, 1224
XhoI CTCGAG 1 cut(s) 73
XspI CTAG 2 cut(s) 1029, 1404
ZrmI AGTACT 1 cut(s) 292
Zsp2I ATGCAT 1 cut(s) 1309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.