Rw0G006150

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00235
OG0003425
Physical Location & Seq
Reverse (-)
168660 .. 170080
1421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G006150.1

Sequence Viewer

Length: 1359 bp
ATGGCAGAAGTTGGAGAAAGCTCTGGTGCAAAGGCCTCTGGTGAAATCTGGGCCAAGCTTATCAGAGATCTTGTTCTGACTCAAGATAACCCTCAGAGTCAAGATATTGGTTCAGATGAGAGTTTAGTTTGCTCTCCTATTGACAAATGCGAGTGGTGCAGAATTACGAGGAACTCCGATCAGTCTTCTGCCACTATACAAAATAAGAGTTCAAATGCAATACTTGTTGATGGAGCTGCGATCCAATCTGAAGAGAGTACTGTTATCAGATCTGGAAGTGAAATTATTACAGGTCTTGAGAGAGAAGGGTGTTTAAGCTACAGATTCAATGTGTTGCTTGGTCCTGAAACTTGTCAGAAGATGCTGAAGATTAGTGTGGATGCTGAGCATGCAAAGTGCTGCATTTGTTTAAATATATGGCATGATGCTGTTACCATTGCTCCTTGCTTCCATAACTTCTGCAATGGGTGCTTCTCAGAGTGGTTAAGGAGATCACAGGAGAAACGTTCAAGTGTACTCTGTCCCCAATGTCGAGCAGTTGTGCAATTTGTTGGAAGAAACCACTTCCTGCATAACATTGCCGATGAAATACTGAATGCGGATTCTTCACTGAGACGCTCCAACGAAGACACTGCAGTTTTAGATTCCTATGCAACAGTACGATCAAATCTGGTAATAAAGTCGGGAAAGGTGCAAGATGAAGCTGATAGTGTAGAGCTTCCCTGCCCTCAATGCAGTAATCAATTTGGTGGGTTTCTCTGCAACCGAAGGACTACCCATCTGCAATGTCAAGCATGTGGAGGAATGATGCCTTCACGAACCAATACAAGTGTACCTCAACACTGTATAGGGTGTGATAGGTCATTTTGCGGTGCATATTGGCATGCTCAAGGCGTTGCACAAAGTGCTTCTCACCACATATGCAGTCGCGATACTTTCAAACCAATCACAGAACGCACCATCTCTAGAATTCCTACTTCAGCACATGAAAACAATCGCCATGAACAAGAAATCACTATGAGGTGTATTGCACAAATGGGAAGAACATTGCAGGATGTTATTGCAGAATGGATGGTAATGTTCAATAACAGAACAATTGATCAAACTAACAGGATGCCACTGAAGCATGCCGAGCTGATAACTTCTGGGACATATACATGCAGTGATTGTTATGAGAAACTGATCGCATTCCTCTTGTACTGGTTTCGGGTTTCAGTTTCTGCATCTCATCTTCCCCCAGATGCATCAAAAAGGGAAAATTGCTGGTATGGATATGCATGTCGAACACAGCACCATAATGAAGACCATGCTCGTAAAAGGAATCATGTATGCCGGCCGACTAGGGGTGCTAATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000209 GO:0000278 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016604 GO:0016605 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031647 GO:0031648 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0036094 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045732 GO:0045786 GO:0045862 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051726 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0097159 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1903047 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_2

Protein Analysis

452

Amino Acids

50.66

Weight (kDa)

6.97

Isoelectric Point (pI)

53.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 132 - 178 3.3e-07 Ring finger domain
zf-C3HC4_2 PF13923 132 - 177 9.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 133 - 177 2.6e-06 Zinc finger, C3HC4 type (RING finger)
zf-CRD PF17979 242 - 405 1.4e-47 Cysteine rich domain with multizinc binding regions
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 930
AciI CCGC 2 cut(s) 599, 870
AclI AACGTT 1 cut(s) 505
AclWI GGATC 1 cut(s) 235
AcoI YGGCCR 1 cut(s) 1334
AcsI RAATTY 1 cut(s) 969
AcuI CTGAAG 4 cut(s) 270, 386, 963, 1142
AdeI CACNNNGTG 1 cut(s) 905
AfaI GTAC 5 cut(s) 259, 516, 660, 834, 1199
AfiI CCNNNNNNNGG 1 cut(s) 1343
AgsI TTSAA 5 cut(s) 213, 328, 510, 940, 1084
AluBI AGCT 7 cut(s) 21, 58, 236, 318, 704, 718, 1135
AluI AGCT 7 cut(s) 21, 58, 236, 318, 704, 718, 1135
Alw26I GTCTC 1 cut(s) 607
AlwI GGATC 1 cut(s) 235
AlwNI CAGNNNCTG 1 cut(s) 1220
AoxI GGCC 3 cut(s) 33, 51, 1334
ApeKI GCWGC 2 cut(s) 236, 399
ApoI RAATTY 1 cut(s) 969
AspS9I GGNCC 2 cut(s) 51, 341
AsuHPI GGTGA 2 cut(s) 53, 905
AvaII GGWCC 1 cut(s) 341
BaeI ACNNNNGTAYC 2 cut(s) 816, 849
BbsI GAAGAC 3 cut(s) 177, 633, 1308
BbvI GCAGC 2 cut(s) 223, 386
BccI CCATC 4 cut(s) 224, 786, 968, 1066
BcgI CGANNNNNNTGC 2 cut(s) 614, 648
BclI TGATCA 1 cut(s) 1099
BcoDI GTCTC 1 cut(s) 607
BfaI CTAG 2 cut(s) 966, 1341
BfmI CTRYAG 2 cut(s) 319, 633
BglII AGATCT 2 cut(s) 67, 269
BisI GCNGC 2 cut(s) 237, 400
BlpI GCTNAGC 1 cut(s) 384
BlsI GCNGC 2 cut(s) 238, 401
BmcAI AGTACT 1 cut(s) 259
Bme18I GGWCC 1 cut(s) 341
BmgT120I GGNCC 2 cut(s) 51, 341
BmsI GCATC 8 cut(s) 351, 370, 415, 798, 1104, 1231, 1232, 1253
BpiI GAAGAC 3 cut(s) 177, 633, 1308
Bpu1102I GCTNAGC 1 cut(s) 384
BpuEI CTTGAG 3 cut(s) 66, 317, 873
BsaXI ACNNNNNCTCC 2 cut(s) 424, 454
Bsc4I CCNNNNNNNGG 1 cut(s) 1343
Bse118I RCCGGY 1 cut(s) 1332
Bse1I ACTGG 1 cut(s) 1205
Bse3DI GCAATG 5 cut(s) 435, 469, 576, 791, 1046
BseGI GGATG 4 cut(s) 385, 1060, 1077, 1119
BseLI CCNNNNNNNGG 1 cut(s) 1343
BseMI GCAATG 5 cut(s) 435, 469, 576, 791, 1046
BseMII CTCAG 4 cut(s) 107, 375, 489, 602
BseNI ACTGG 1 cut(s) 1205
BseX3I CGGCCG 1 cut(s) 1334
BseXI GCAGC 2 cut(s) 223, 386
BsgI GTGCAG 1 cut(s) 178
Bsh1236I CGCG 1 cut(s) 930
Bsh1285I CGRYCG 1 cut(s) 1337
BshFI GGCC 3 cut(s) 35, 53, 1336
BsiEI CGRYCG 1 cut(s) 1337
BsiSI CCGG 1 cut(s) 1333
BslFI GGGAC 2 cut(s) 507, 1162
BslI CCNNNNNNNGG 1 cut(s) 1343
BsmAI GTCTC 1 cut(s) 607
BsmBI CGTCTC 1 cut(s) 607
BsmFI GGGAC 2 cut(s) 507, 1162
BsmI GAATGC 2 cut(s) 601, 1187
BsnI GGCC 3 cut(s) 35, 53, 1336
Bsp143I GATC 8 cut(s) 67, 178, 240, 269, 491, 662, 1099, 1182
Bsp1720I GCTNAGC 1 cut(s) 384
Bsp68I TCGCGA 1 cut(s) 930
BspACI CCGC 2 cut(s) 599, 870
BspANI GGCC 3 cut(s) 35, 53, 1336
BspCNI CTCAG 4 cut(s) 106, 376, 488, 603
BspFNI CGCG 1 cut(s) 930
BspMAI CTGCAG 1 cut(s) 637
BspPI GGATC 1 cut(s) 235
BsrDI GCAATG 5 cut(s) 435, 469, 576, 791, 1046
BsrFI RCCGGY 1 cut(s) 1332
BsrI ACTGG 1 cut(s) 1205
BssAI RCCGGY 1 cut(s) 1332
BssMI GATC 8 cut(s) 67, 178, 240, 269, 491, 662, 1099, 1182
Bst4CI ACNGT 3 cut(s) 262, 658, 845
Bst6I CTCTTC 1 cut(s) 246
BstAPI GCANNNNNTGC 2 cut(s) 468, 905
BstC8I GCNNGC 4 cut(s) 390, 885, 1128, 1334
BstDEI CTNAG 4 cut(s) 93, 384, 475, 611
BstF5I GGATG 4 cut(s) 385, 1060, 1077, 1119
BstFNI CGCG 1 cut(s) 930
BstKTI GATC 8 cut(s) 70, 181, 243, 272, 494, 665, 1102, 1185
BstMAI GTCTC 1 cut(s) 607
BstMBI GATC 8 cut(s) 67, 178, 240, 269, 491, 662, 1099, 1182
BstMCI CGRYCG 1 cut(s) 1337
BstMWI GCNNNNNNNGC 7 cut(s) 156, 389, 468, 732, 905, 1123, 1132
BstNSI RCATGY 6 cut(s) 392, 798, 887, 1130, 1161, 1281
BstSFI CTRYAG 2 cut(s) 319, 633
BstUI CGCG 1 cut(s) 930
BstV1I GCAGC 2 cut(s) 223, 386
BstV2I GAAGAC 3 cut(s) 177, 633, 1308
BstX2I RGATCY 2 cut(s) 67, 269
BstYI RGATCY 2 cut(s) 67, 269
BstZI CGGCCG 1 cut(s) 1334
BsuRI GGCC 3 cut(s) 35, 53, 1336
BtsCI GGATG 4 cut(s) 385, 1060, 1077, 1119
BtsI GCAGTG 2 cut(s) 630, 1168
BtsIMutI CAGTG 5 cut(s) 608, 630, 841, 1118, 1168
BtuMI TCGCGA 1 cut(s) 930
Cac8I GCNNGC 4 cut(s) 390, 885, 1128, 1334
CaiI CAGNNNCTG 1 cut(s) 1220
Cfr10I RCCGGY 1 cut(s) 1332
Cfr13I GGNCC 2 cut(s) 51, 341
CseI GACGC 1 cut(s) 624
Csp6I GTAC 5 cut(s) 258, 515, 659, 833, 1198
CviQI GTAC 5 cut(s) 258, 515, 659, 833, 1198
DdeI CTNAG 4 cut(s) 93, 384, 475, 611
DpnI GATC 8 cut(s) 69, 180, 242, 271, 493, 664, 1101, 1184
DpnII GATC 8 cut(s) 67, 178, 240, 269, 491, 662, 1099, 1182
DraI TTTAAA 1 cut(s) 411
DraIII CACNNNGTG 1 cut(s) 905
EaeI YGGCCR 1 cut(s) 1334
EagI CGGCCG 1 cut(s) 1334
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
EclXI CGGCCG 1 cut(s) 1334
Eco147I AGGCCT 1 cut(s) 35
Eco47I GGWCC 1 cut(s) 341
Eco52I CGGCCG 1 cut(s) 1334
Eco57I CTGAAG 4 cut(s) 270, 386, 963, 1142
EcoRI GAATTC 1 cut(s) 969
EcoT22I ATGCAT 2 cut(s) 1246, 1279
Esp3I CGTCTC 1 cut(s) 607
FaqI GGGAC 2 cut(s) 507, 1162
FauNDI CATATG 1 cut(s) 920
FbaI TGATCA 1 cut(s) 1099
Fnu4HI GCNGC 2 cut(s) 237, 400
FokI GGATG 4 cut(s) 392, 1067, 1084, 1126
Fsp4HI GCNGC 2 cut(s) 237, 400
FspBI CTAG 2 cut(s) 966, 1341
GluI GCNGC 2 cut(s) 237, 400
HaeIII GGCC 3 cut(s) 35, 53, 1336
HapII CCGG 1 cut(s) 1333
HgaI GACGC 1 cut(s) 624
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 6 cut(s) 79, 97, 324, 602, 644, 1321
HpaII CCGG 1 cut(s) 1333
HphI GGTGA 2 cut(s) 53, 905
Hpy166II GTNNAC 2 cut(s) 515, 833
Hpy188I TCNGA 9 cut(s) 65, 78, 96, 115, 178, 250, 269, 357, 478
Hpy188III TCNNGA 9 cut(s) 83, 101, 273, 296, 344, 684, 816, 929, 966
Hpy8I GTNNAC 2 cut(s) 515, 833
HpyAV CCTTC 3 cut(s) 299, 762, 822
HpyCH4III ACNGT 3 cut(s) 262, 658, 845
HpyCH4IV ACGT 1 cut(s) 505
HpyF10VI GCNNNNNNNGC 7 cut(s) 156, 389, 468, 732, 905, 1123, 1132
HpyF3I CTNAG 4 cut(s) 93, 384, 475, 611
HpySE526I ACGT 1 cut(s) 505
KroI GCCGGC 1 cut(s) 1332
KroNI GCCGGC 1 cut(s) 1334
Ksp22I TGATCA 1 cut(s) 1099
Kzo9I GATC 8 cut(s) 67, 178, 240, 269, 491, 662, 1099, 1182
LmnI GCTCC 3 cut(s) 233, 445, 623
Lsp1109I GCAGC 2 cut(s) 223, 386
LweI GCATC 8 cut(s) 351, 370, 415, 798, 1104, 1231, 1232, 1253
MaeI CTAG 2 cut(s) 966, 1341
MaeII ACGT 1 cut(s) 505
MaeIII GTNAC 1 cut(s) 430
MalI GATC 8 cut(s) 69, 180, 242, 271, 493, 664, 1101, 1184
MboI GATC 8 cut(s) 67, 178, 240, 269, 491, 662, 1099, 1182
MfeI CAATTG 1 cut(s) 1095
MflI RGATCY 2 cut(s) 67, 269
MluCI AATT 7 cut(s) 162, 282, 545, 743, 969, 1095, 1258
MlyI GAGTC 2 cut(s) 73, 106
MmeI TCCRAC 2 cut(s) 532, 645
MnlI CCTC 8 cut(s) 46, 102, 162, 738, 794, 846, 1014, 1202
Mph1103I ATGCAT 2 cut(s) 1246, 1279
MroNI GCCGGC 1 cut(s) 1332
MseI TTAA 3 cut(s) 314, 410, 485
MslI CAYNNNNRTG 2 cut(s) 1156, 1296
MspI CCGG 1 cut(s) 1333
MunI CAATTG 1 cut(s) 1095
Mva1269I GAATGC 2 cut(s) 601, 1187
MvnI CGCG 1 cut(s) 930
MwoI GCNNNNNNNGC 7 cut(s) 156, 389, 468, 732, 905, 1123, 1132
NaeI GCCGGC 1 cut(s) 1334
NdeI CATATG 1 cut(s) 920
NdeII GATC 8 cut(s) 67, 178, 240, 269, 491, 662, 1099, 1182
NgoMIV GCCGGC 1 cut(s) 1332
NmeAIII GCCGAG 1 cut(s) 1156
NruI TCGCGA 1 cut(s) 930
NsiI ATGCAT 2 cut(s) 1246, 1279
NspI RCATGY 6 cut(s) 392, 798, 887, 1130, 1161, 1281
PaeI GCATGC 3 cut(s) 392, 887, 1130
PceI AGGCCT 1 cut(s) 35
PctI GAATGC 2 cut(s) 601, 1187
PdiI GCCGGC 1 cut(s) 1334
PfeI GAWTC 4 cut(s) 324, 602, 644, 1321
PkrI GCNGC 2 cut(s) 238, 401
PleI GAGTC 2 cut(s) 73, 105
PpsI GAGTC 2 cut(s) 73, 105
Psp1406I AACGTT 1 cut(s) 505
PspPI GGNCC 2 cut(s) 51, 341
PstI CTGCAG 1 cut(s) 637
PstNI CAGNNNCTG 1 cut(s) 1220
PsuI RGATCY 2 cut(s) 67, 269
RruI TCGCGA 1 cut(s) 930
RsaI GTAC 5 cut(s) 259, 516, 660, 834, 1199
RsaNI GTAC 5 cut(s) 258, 515, 659, 833, 1198
RseI CAYNNNNRTG 2 cut(s) 1156, 1296
SaqAI TTAA 3 cut(s) 314, 410, 485
SatI GCNGC 2 cut(s) 237, 400
Sau3AI GATC 8 cut(s) 67, 178, 240, 269, 491, 662, 1099, 1182
Sau96I GGNCC 2 cut(s) 51, 341
ScaI AGTACT 1 cut(s) 259
SchI GAGTC 2 cut(s) 73, 106
SfaNI GCATC 8 cut(s) 351, 370, 415, 798, 1104, 1231, 1232, 1253
SfcI CTRYAG 2 cut(s) 319, 633
SinI GGWCC 1 cut(s) 341
SmiMI CAYNNNNRTG 2 cut(s) 1156, 1296
SmlI CTYRAG 3 cut(s) 81, 296, 888
SmoI CTYRAG 3 cut(s) 81, 296, 888
SphI GCATGC 3 cut(s) 392, 887, 1130
Sse9I AATT 7 cut(s) 162, 282, 545, 743, 969, 1095, 1258
SseBI AGGCCT 1 cut(s) 35
SsiI CCGC 2 cut(s) 599, 870
SspMI CTAG 2 cut(s) 966, 1341
StuI AGGCCT 1 cut(s) 35
TaaI ACNGT 3 cut(s) 262, 658, 845
TaiI ACGT 1 cut(s) 508
TaqI TCGA 2 cut(s) 532, 1282
TasI AATT 7 cut(s) 162, 282, 545, 743, 969, 1095, 1258
TatI WGTACW 3 cut(s) 257, 514, 1197
TfiI GAWTC 4 cut(s) 324, 602, 644, 1321
Tru1I TTAA 3 cut(s) 314, 410, 485
Tru9I TTAA 3 cut(s) 314, 410, 485
TscAI CASTG 5 cut(s) 615, 637, 848, 1125, 1168
TseI GCWGC 2 cut(s) 236, 399
TspDTI ATGAA 5 cut(s) 600, 714, 1002, 1017, 1314
TspRI CASTG 5 cut(s) 615, 637, 848, 1125, 1168
VpaK11BI GGWCC 1 cut(s) 341
XapI RAATTY 1 cut(s) 969
XbaI TCTAGA 1 cut(s) 965
XceI RCATGY 6 cut(s) 392, 798, 887, 1130, 1161, 1281
XspI CTAG 2 cut(s) 966, 1341
ZrmI AGTACT 1 cut(s) 259
Zsp2I ATGCAT 2 cut(s) 1246, 1279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.