MD00G1120800.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0003425
Physical Location & Seq
Reverse (-)
25902525 .. 25903445
921 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1120800.v1.1.491

Sequence Viewer

Length: 615 bp
ATGGCAGAAGTTGGAGAGAGCTCGGGTGCAAAACCCTGTGAAGAATTCTGGGCCAAGCTCGTTCCATCAGACTTGAGATATCCGGATGTTGAAATTATTTCATATGAGATTGTAGTTTGCTCAGAGATCTTTTTATATTGGCATGCGCAAGGGGTTTCACAAAGTGATTCTCACCATGTGTGCAGTCGCGAGACTTTTAAACCAATCTCAGAATGGACCATCTCTGGAATCCCTACTTCAACTCATGAAAACAATCGACATGAACAAGTGATCACTGAGAAGTGTACTGTACAGTTGGGGAGAACATCACAGGATGTTATTGCAGAATGGCTTGCAAAGTTGAACAACAGAGAAATTGATCGAACGAGGATGCCCCTGAATCATGCTGAGGCAATAACTTCTGGGACACCTACGTGCAATTACTGTTATGAGAAGCTGATCTCCTTCCTCTTGTACTGGTTTCGGATTTCGATTCCCAAATATCATCTACCCACAGAGGCATCAAACAGGGAAAATTGCTGGTATGGATATGCATGTCGGACACAGCACCATAATGAAGAACATGCTCGTAAAAGAAATCCTGTGTGCCGGCCAACTAGGGGTACTTATATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000209 GO:0000278 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016604 GO:0016605 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031647 GO:0031648 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0036094 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045732 GO:0045786 GO:0045862 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051726 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0097159 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1903047 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_2

Protein Analysis

205

Amino Acids

23.7

Weight (kDa)

6.36

Isoelectric Point (pI)

40.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CRD PF17979 38 - 158 4.2e-27 Cysteine rich domain with multizinc binding regions
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 147
AccII CGCG 1 cut(s) 189
AccIII TCCGGA 1 cut(s) 82
AcoI YGGCCR 1 cut(s) 590
AcsI RAATTY 1 cut(s) 44
AdeI CACNNNGTG 2 cut(s) 164, 178
AfaI GTAC 4 cut(s) 286, 291, 455, 604
AfiI CCNNNNNNNGG 1 cut(s) 599
AgsI TTSAA 3 cut(s) 92, 240, 343
AleI CACNNNNGTG 1 cut(s) 412
AluBI AGCT 3 cut(s) 21, 58, 436
AluI AGCT 3 cut(s) 21, 58, 436
Alw21I GWGCWC 1 cut(s) 23
Alw26I GTCTC 1 cut(s) 185
Ama87I CYCGRG 1 cut(s) 22
Aor13HI TCCGGA 1 cut(s) 82
AoxI GGCC 2 cut(s) 51, 590
ApoI RAATTY 1 cut(s) 44
AspLEI GCGC 1 cut(s) 148
AspS9I GGNCC 2 cut(s) 51, 216
AsuHPI GGTGA 1 cut(s) 164
AvaI CYCGRG 1 cut(s) 22
AvaII GGWCC 1 cut(s) 216
BanII GRGCYC 1 cut(s) 23
Bbv12I GWGCWC 1 cut(s) 23
BbvCI CCTCAGC 1 cut(s) 387
BccI CCATC 2 cut(s) 73, 227
BclI TGATCA 1 cut(s) 270
BcoDI GTCTC 1 cut(s) 185
BfaI CTAG 1 cut(s) 597
BglII AGATCT 1 cut(s) 126
Bme18I GGWCC 1 cut(s) 216
BmeT110I CYCGRG 1 cut(s) 22
BmgT120I GGNCC 2 cut(s) 51, 216
BmsI GCATC 2 cut(s) 360, 509
Bpu10I CCTNAGC 1 cut(s) 387
BpuEI CTTGAG 1 cut(s) 94
BsaAI YACGTR 1 cut(s) 414
BsaWI WCCGGW 1 cut(s) 82
Bsc4I CCNNNNNNNGG 1 cut(s) 599
Bse118I RCCGGY 1 cut(s) 588
Bse1I ACTGG 1 cut(s) 461
BseAI TCCGGA 1 cut(s) 82
BseGI GGATG 3 cut(s) 91, 319, 375
BseLI CCNNNNNNNGG 1 cut(s) 599
BseMII CTCAG 4 cut(s) 135, 222, 267, 378
BseNI ACTGG 1 cut(s) 461
BsgI GTGCAG 1 cut(s) 202
Bsh1236I CGCG 1 cut(s) 189
BshFI GGCC 2 cut(s) 53, 592
BsiHKAI GWGCWC 1 cut(s) 23
BsiHKCI CYCGRG 1 cut(s) 22
BsiSI CCGG 2 cut(s) 83, 589
BslFI GGGAC 1 cut(s) 418
BslI CCNNNNNNNGG 1 cut(s) 599
BsmAI GTCTC 1 cut(s) 185
BsmFI GGGAC 1 cut(s) 418
BsnI GGCC 2 cut(s) 53, 592
BsoBI CYCGRG 1 cut(s) 22
Bsp1286I GDGCHC 1 cut(s) 23
Bsp13I TCCGGA 1 cut(s) 82
Bsp1407I TGTACA 1 cut(s) 289
Bsp143I GATC 4 cut(s) 126, 270, 358, 438
Bsp68I TCGCGA 1 cut(s) 189
BspANI GGCC 2 cut(s) 53, 592
BspCNI CTCAG 4 cut(s) 134, 221, 268, 379
BspEI TCCGGA 1 cut(s) 82
BspFNI CGCG 1 cut(s) 189
BspHI TCATGA 1 cut(s) 244
BsrFI RCCGGY 1 cut(s) 588
BsrGI TGTACA 1 cut(s) 289
BsrI ACTGG 1 cut(s) 461
BssAI RCCGGY 1 cut(s) 588
BssMI GATC 4 cut(s) 126, 270, 358, 438
Bst4CI ACNGT 3 cut(s) 289, 294, 425
BstAUI TGTACA 1 cut(s) 289
BstBAI YACGTR 1 cut(s) 414
BstC8I GCNNGC 3 cut(s) 144, 333, 590
BstDEI CTNAG 4 cut(s) 121, 208, 276, 387
BstF5I GGATG 3 cut(s) 91, 319, 375
BstFNI CGCG 1 cut(s) 189
BstHHI GCGC 1 cut(s) 148
BstKTI GATC 4 cut(s) 129, 273, 361, 441
BstMAI GTCTC 1 cut(s) 185
BstMBI GATC 4 cut(s) 126, 270, 358, 438
BstNSI RCATGY 3 cut(s) 146, 537, 566
BstUI CGCG 1 cut(s) 189
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
BsuRI GGCC 2 cut(s) 53, 592
BtsCI GGATG 3 cut(s) 91, 319, 375
BtsIMutI CAGTG 1 cut(s) 273
BtuMI TCGCGA 1 cut(s) 189
Cac8I GCNNGC 3 cut(s) 144, 333, 590
CciI TCATGA 1 cut(s) 244
CfoI GCGC 1 cut(s) 148
Cfr10I RCCGGY 1 cut(s) 588
Cfr13I GGNCC 2 cut(s) 51, 216
Csp6I GTAC 4 cut(s) 285, 290, 454, 603
CviAII CATG 7 cut(s) 143, 176, 245, 260, 383, 534, 563
CviJI RGCY 6 cut(s) 21, 53, 58, 331, 436, 592
CviKI_1 RGCY 6 cut(s) 21, 53, 58, 331, 436, 592
CviQI GTAC 4 cut(s) 285, 290, 454, 603
DdeI CTNAG 4 cut(s) 121, 208, 276, 387
DpnI GATC 4 cut(s) 128, 272, 360, 440
DpnII GATC 4 cut(s) 126, 270, 358, 438
DraI TTTAAA 1 cut(s) 199
DraIII CACNNNGTG 2 cut(s) 164, 178
EaeI YGGCCR 1 cut(s) 590
Ecl136II GAGCTC 1 cut(s) 21
Eco24I GRGCYC 1 cut(s) 23
Eco32I GATATC 1 cut(s) 80
Eco47I GGWCC 1 cut(s) 216
Eco53kI GAGCTC 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 21
EcoRI GAATTC 1 cut(s) 44
EcoRV GATATC 1 cut(s) 80
EcoT22I ATGCAT 1 cut(s) 535
EcoT38I GRGCYC 1 cut(s) 23
FaeI CATG 7 cut(s) 146, 179, 248, 263, 386, 537, 566
FaqI GGGAC 1 cut(s) 418
FatI CATG 7 cut(s) 142, 175, 244, 259, 382, 533, 562
FauNDI CATATG 1 cut(s) 103
FbaI TGATCA 1 cut(s) 270
FokI GGATG 3 cut(s) 98, 326, 382
FriOI GRGCYC 1 cut(s) 23
FspBI CTAG 1 cut(s) 597
FspI TGCGCA 1 cut(s) 147
GlaI GCGC 1 cut(s) 147
HaeIII GGCC 2 cut(s) 53, 592
HapII CCGG 2 cut(s) 83, 589
HhaI GCGC 1 cut(s) 148
Hin1II CATG 7 cut(s) 146, 179, 248, 263, 386, 537, 566
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HinfI GANTC 4 cut(s) 167, 228, 379, 472
HpaII CCGG 2 cut(s) 83, 589
HphI GGTGA 1 cut(s) 164
Hpy166II GTNNAC 1 cut(s) 285
Hpy188I TCNGA 5 cut(s) 70, 124, 211, 465, 540
Hpy188III TCNNGA 4 cut(s) 83, 188, 225, 245
Hpy8I GTNNAC 1 cut(s) 285
HpyAV CCTTC 1 cut(s) 454
HpyCH4III ACNGT 3 cut(s) 289, 294, 425
HpyCH4IV ACGT 1 cut(s) 413
HpyCH4V TGCA 6 cut(s) 29, 183, 323, 335, 417, 533
HpyF3I CTNAG 4 cut(s) 121, 208, 276, 387
HpySE526I ACGT 1 cut(s) 413
Hsp92II CATG 7 cut(s) 146, 179, 248, 263, 386, 537, 566
HspAI GCGC 1 cut(s) 146
Kpn2I TCCGGA 1 cut(s) 82
KroI GCCGGC 1 cut(s) 588
KroNI GCCGGC 1 cut(s) 590
Ksp22I TGATCA 1 cut(s) 270
Kzo9I GATC 4 cut(s) 126, 270, 358, 438
LweI GCATC 2 cut(s) 360, 509
MaeI CTAG 1 cut(s) 597
MaeII ACGT 1 cut(s) 413
MalI GATC 4 cut(s) 128, 272, 360, 440
MboI GATC 4 cut(s) 126, 270, 358, 438
MboII GAAGA 2 cut(s) 53, 569
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 1 cut(s) 23
MluCI AATT 5 cut(s) 44, 93, 354, 418, 514
MmeI TCCRAC 1 cut(s) 518
MnlI CCTC 4 cut(s) 360, 382, 458, 490
Mph1103I ATGCAT 1 cut(s) 535
MroI TCCGGA 1 cut(s) 82
MroNI GCCGGC 1 cut(s) 588
MseI TTAA 1 cut(s) 198
MslI CAYNNNNRTG 2 cut(s) 412, 552
MspI CCGG 2 cut(s) 83, 589
MvnI CGCG 1 cut(s) 189
NaeI GCCGGC 1 cut(s) 590
NdeI CATATG 1 cut(s) 103
NdeII GATC 4 cut(s) 126, 270, 358, 438
NgoMIV GCCGGC 1 cut(s) 588
NlaIII CATG 7 cut(s) 146, 179, 248, 263, 386, 537, 566
NruI TCGCGA 1 cut(s) 189
NsbI TGCGCA 1 cut(s) 147
NsiI ATGCAT 1 cut(s) 535
NspI RCATGY 3 cut(s) 146, 537, 566
OliI CACNNNNGTG 1 cut(s) 412
PaeI GCATGC 1 cut(s) 146
PagI TCATGA 1 cut(s) 244
PdiI GCCGGC 1 cut(s) 590
PfeI GAWTC 4 cut(s) 167, 228, 379, 472
Ppu21I YACGTR 1 cut(s) 414
Psp124BI GAGCTC 1 cut(s) 23
PspPI GGNCC 2 cut(s) 51, 216
PsuI RGATCY 1 cut(s) 126
RruI TCGCGA 1 cut(s) 189
RsaI GTAC 4 cut(s) 286, 291, 455, 604
RsaNI GTAC 4 cut(s) 285, 290, 454, 603
RseI CAYNNNNRTG 2 cut(s) 412, 552
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 1 cut(s) 198
Sau3AI GATC 4 cut(s) 126, 270, 358, 438
Sau96I GGNCC 2 cut(s) 51, 216
SduI GDGCHC 1 cut(s) 23
SetI ASST 5 cut(s) 23, 60, 412, 416, 438
SfaNI GCATC 2 cut(s) 360, 509
SinI GGWCC 1 cut(s) 216
SmiMI CAYNNNNRTG 2 cut(s) 412, 552
SmlI CTYRAG 1 cut(s) 73
SmoI CTYRAG 1 cut(s) 73
SphI GCATGC 1 cut(s) 146
Sse9I AATT 5 cut(s) 44, 93, 354, 418, 514
SspMI CTAG 1 cut(s) 597
SstI GAGCTC 1 cut(s) 23
TaaI ACNGT 3 cut(s) 289, 294, 425
TaiI ACGT 1 cut(s) 416
TaqI TCGA 3 cut(s) 256, 361, 470
TasI AATT 5 cut(s) 44, 93, 354, 418, 514
TatI WGTACW 3 cut(s) 284, 289, 453
TfiI GAWTC 4 cut(s) 167, 228, 379, 472
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TscAI CASTG 1 cut(s) 280
TspDTI ATGAA 4 cut(s) 90, 261, 276, 570
TspRI CASTG 1 cut(s) 280
VpaK11BI GGWCC 1 cut(s) 216
XapI RAATTY 1 cut(s) 44
XceI RCATGY 3 cut(s) 146, 537, 566
XcmI CCANNNNNNNNNTGG 1 cut(s) 210
XspI CTAG 1 cut(s) 597
Zsp2I ATGCAT 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.